KR20230165406A - Body holder and tail fixing device for tail caudal vein administration in laboratory mouse - Google Patents

Abstract

The present invention relates to a body holder and a tail correction device for administering the tail caudal vein of a laboratory rat, which assists blood collection from the tail after fixing the laboratory rat. After placing the experimental animal on the upper part of the seating plate 100, the tail portion can be easily fixed separately for blood vessel sampling or drug injection of the caudal vein or caudal artery. In particular, not only can experimental animals be fixed very quickly and accurately, but also the tail can be easily fixed in a bent state of about 90 degrees, which will improve the repeatability and accuracy of the experimenter's experiment. .

Description

Body holder and tail fixing device for tail caudal vein administration in laboratory mouse}

The present invention relates to a body holder and tail correction device for administering the tail caudal vein of a laboratory rat, which assists blood collection from the tail after fixing the laboratory rat. The present invention relates to a body holder and a tail correction device for administration to the tail caudal vein of a laboratory rat that can be used to selectively insert a needle into an artery and collect blood.

In general, intravenous injection has many advantages over other routes. Because the medicinal solution passes through the heart within seconds and reaches the necessary tissues of the body, the medicinal effect appears quickly and the response is more certain. It is used to supply moisture, salt, and other necessary electrolytes in cases where sufficient moisture cannot be administered orally, to supply blood after bleeding, to inject drugs such as antidotes into the blood, or to expect rapid drug efficacy.

Due to these characteristics, newly developed drugs or contrast agents are injected into experimental animals. In the field of new drug development and basic research, laboratory animals are used for various purposes, and as the research purposes and subjects vary, various types of calibration devices for application to experiments with laboratory animal models are being proposed.

Among the above experimental animals, laboratory rats are used relatively frequently, and at this time, intravenous injection is essential, and the rat is usually held with one hand and injected into the tail with the other hand.

According to Korean Registered Utility Model No. 20-0329108, laboratory rats have thin and weak blood vessels, and due to the fluctuation of the tail, technical skill is required and it is difficult for one person to inject. Therefore, in the past, an auxiliary device was designed to secure the torso of an experimental rat inside a container, but its use was limited because the torso was placed inside the container so that only the tail vein was secured rather than fixing the experimental rat conveniently and stably. .

In addition, when drugs are injected not only into veins but also into arteries in the tail, it is difficult to identify and find arterial blood vessels, making it difficult to obtain precise experimental data.

Korean Patent Publication No. 10-1725639 (Auxiliary device for caudal vein injection and blood collection of laboratory rats, registered on April 4, 2017)

The present invention was devised to solve the above problems. The present invention was developed to quickly and stably fix the body of the experimental rat and remove only the tail separately to limit the movement of the tail, thereby enabling precise injection into the caudal vein of the experimental rat. The purpose is to provide a body holder and tail correction device for.

The present invention includes a seating plate (100) on which a laboratory animal with a tail is placed; A fixing part 200 coupled to the seating plate 100 to restrain the experimental animal placed on the seating plate 100; A position control unit 300 coupled to one side of the seating plate 100 to support the seating plate 100; and at least one guide plate 400 coupled to the upper surface of the position control unit 300 and allowing the experimenter to determine and restrain the bending direction of the tail; Includes.

The seating plate 100 of the present invention may be rotatably coupled to the rotation axis 110 coupled to the position control unit 300.

The seating plate 100 of the present invention further includes a rotation lever 120 provided with a tail insertion hole 121 into which the tail of the experimental animal is inserted and fixed, and the tail is moved by rotation of the rotation lever 120. A predetermined angle may also be rotated.

The position control unit 300 of the present invention may be hinged with a support arm 600 fixed to one side of the position control unit 300 so that it can rotate by a predetermined angle relative to the ground.

The guide plate 400 of the present invention is a first guide plate 410 fixed to one upper surface of the position control unit 300 and a first guide groove 440 where the tail is disposed. It may include a second guide plate (420) spaced apart from the plate 410 by a predetermined distance and fixed to the upper surface of the other side of the position control unit (300).

In order to provide a second guide groove 450 for changing the direction of the tail, the first or second guide plate 410, 420 of the present invention is provided on the lower side of the first or second guide plate 410, 420. It may include a third guide plate 430 that is spaced apart from the 420 by a predetermined distance and is fixed to the upper surface of the other side of the position control unit 300.

The fixing part 200 of the present invention includes a jacket 210 to be worn on the body of the experimental animal and a boa system 220 for bringing the jacket 210 into close contact with the seating plate 100, or the fixing part 200 is coupled to the seating plate 100 and may be a mold 230 for pressing the body of the experimental animal.

The present invention has the advantage of restraining the body movement of the test subject and minimizing the movement of the tail, so that when injecting a drug into the tail, the injection can be accurately performed into the blood vessel, and the angle and height of the test subject and the tail can be adjusted. .

In addition, the present invention can easily find and inject veins or arteries in the tail due to the configuration of the specimen seating plate and guide plate that can be rotated at a certain angle.

Figure 1 is a conceptual diagram showing a caudal vein blood collection system including a laboratory animal calibration device according to the present invention.
Figure 2 is a conceptual diagram showing an embodiment related to a guide plate among the laboratory animal correction devices according to the present invention.
Figure 3 is a conceptual diagram showing another embodiment related to the guide plate among the laboratory animal correction devices according to the present invention.
Figure 4 is a plan conceptual diagram showing another embodiment related to the guide plate among the laboratory animal correction devices according to the present invention.
Figure 5 is a conceptual diagram related to the rotation lever among the laboratory animal correction devices according to the present invention.
Figure 6 is a conceptual diagram of a heating device for dilating mouse tail blood vessels according to the present invention.

Since the present invention can be modified in various ways and have various embodiments, the preferred embodiments will be described in detail. It is obvious to those skilled in the art that the present invention can be embodied in other specific forms without departing from the spirit and essential features of the present invention.

Unless otherwise defined, all technical and chemical terms used in this specification have the same meaning as commonly understood by an expert skilled in the technical field to which the present invention pertains. In general, the nomenclature used herein and the experimental methods described below are well known and commonly used in the art.

1 is an overall conceptual diagram of a caudal vein blood collection or administration system. The laboratory animal calibration device according to the present invention is shown to collect blood from the caudal vein of a laboratory animal, particularly a laboratory rat, fixed to the laboratory animal calibration device according to the present invention. Near-infrared rays are irradiated to the upper side of the caudal vein of an experimental animal fixed to a laboratory animal correction device, and the location of the caudal vein is accurately determined using an optical microscope. It can be enlarged using a digital camera connected to an optical microscope, and the experimenter can check it with the naked eye through a digital viewer.

Using a near-infrared irradiation device, thermal action and vascular imaging are possible, and using an optical microscope, the caudal vein of a mouse can be magnified 10 to 100 times, and the position of the injection needle can be confirmed using a digital viewer (LCD screen) while injecting specific drugs into the caudal vein. It can be administered or blood collected.

Below, the laboratory animal calibration device will be described in detail. This will be described in detail with reference to FIGS. 1 to 3.

The present invention includes a seating plate 100, a fixing unit 200, a position control unit 300, a guide plate 400, and a heating unit 500.

A support arm 600 and a base 700 to support and fix them may be further included.

Referring to FIG. 1, the base 700 is placed on the ground or an experimental table and is used for support. A support arm 600 for fixing the position control unit 300, which will be described later, is fixed to the upper part of the base 700. The support arm 600 may be fixed at an angle inclined at a predetermined angle based on the base 700 in consideration of user usability. The slope can be arbitrarily designed considering the convenience of the experimenter.

Referring to Figures 1 to 3, a position control unit 300, etc. is provided on the upper part of the support arm 600.

[Position control unit (300)]

Referring to FIG. 1, the position control unit 300 serves as a base on which an experimental animal placed on a seating plate 100, which will be described later, is fixed. The position control unit 300 may be hinged (not shown) with the support arm 600 described above and tilted at a predetermined angle. In other words, the purpose is to determine the position of the caudal vein or make the experiment easier by adjusting the inclination of the position control unit 300 according to the experimenter's inclination. Since various known techniques may be applied in relation to hinge coupling, specific details are avoided.

[Guide Plate (400)]

1 to 4, the guide plate 400 of the present invention will be described in detail.

The guide plate 400 is disposed on the upper surface of the position control unit 300 described above. As shown, a plurality of holes or protrusions 310 may be arranged regularly or irregularly on the upper surface of the position control unit 300. The guide plate 400 may also have a protrusion or hole 470 disposed at a corresponding position. Alternatively, a magnet may be provided inside all or one of the guide plate 400 and the position control unit 300 so that they can be coupled with a magnet or the like. The above-described holes or protrusions 310 and 470 not only facilitate the coupling between the guide plate 400 and the position control unit 300, but also serve as a guide for the experimenter to determine position for various purposes such as experiments.

Referring to Figures 1 to 3, the guide plate 400 includes first to third guide plates (410, 420, and 430). The guide plate 400, which is made up of at least three plates, can be coupled to the upper surface of the position control unit 300 to naturally form the first and second guide grooves 440 and 450. The tail of an experimental animal is inserted and fixed inside the first and second guide grooves 440 and 450.

In particular, referring to FIG. 3, the first guide plate 410 may be coupled and fixed to one half-split surface of the position control unit 300.

Among the other half-split surfaces, the upper split surface is fixed to the second guide plate 420, and the lower split surface is fixed to the third guide plate 430 in conjunction with the position control unit 300. The first and second guide grooves 440 and 450 as described above may be formed between them (see FIGS. 1 and 2). Alternatively, referring to FIGS. 3 and 4, the second guide groove 450 may be cut so that the ends of the second and third guide plates 420 and 430 have a 'base (ㄱ)' shape. The second guide groove 450 is naturally formed by combining the second and third guide plates 420 and 430. The tail of the experimental animal can be seated and fixed in the second guide groove 450.

Meanwhile, as shown in Figures 2 and 4, the experimenter can arbitrarily change the positions of the first to third guide plates (410, 420, and 430) for the convenience of the experiment. In particular, the second and third guide plates 420 and 430 may be manufactured symmetrically. For this reason, it is desirable to ensure that the second and third guide plates 420 and 430 can be fastened to the position control unit 300 in the same shape or perform the same function even if the second and third guide plates 420 and 430 are turned over or moved to the left and right.

Referring to Figures 3 and 4, a pair of guide rollers 460 can be placed in the area where the first guide groove 440 bends to the second guide groove 450 so that the tail of the experimental animal can be firmly fixed. . The guide roller 460 may be composed of a core 461 centered on a shaft 462 and a louver 461 surrounding the core 461. The louver 461 is a part where the tail of the experimental animal comes into contact, and may be made of a non-slip material, such as rubber.

As shown in Figures 1 to 4, it is preferable to fix the tail in a state bent at approximately 90 degrees in order to improve the accuracy of experiments such as accurate caudal vein check and blood collection using the above-described configuration.

Additionally, to improve the accuracy of the experiment, it is desirable to raise the temperature of the tail portion, so it is desirable for the position control unit 300, guide plates 400, 410, 420, 430, etc. to be heated. For this purpose, it is preferable that a heating unit 500 is provided between the position control unit 300 and the guide plates 400, 410, 420, and 430. Alternatively, the heating unit 500 may be provided on the inner side of the position control unit 300. In other words, since the heating unit 500 has the purpose of raising the temperature of the tail portion of the experimental animal, any location for increasing the temperature may be used.

[Seat plate (100)]

Referring to Figures 1 to 3, the seating plate 100 is rotatably fixed to one side of the position control unit 300. As shown in FIG. 3, the rotation axis 110 is fixed to one side of the position control unit 300. The experimental animal is rotated by rotating the seating plate 100 on which the body of the experimental animal is fixed. The position of the caudal vein or caudal artery can be accurately changed according to the rotation of the experimental animal.

Referring to Figure 5, a rotation lever 120 is provided on one side of the seating plate 100. The rotation lever 120 can be rotated independently or is preferably configured to be rotated integrally with the seating plate 100. It is preferable that the rotation axis 110 is provided at approximately the center of the rotation lever 110, and the tail insertion hole 121 is provided at the upper center side. The tail insertion hole 121 may be formed in a circular or semicircular shape. When only the rotation lever 120 is rotated independently, it is preferable to form a semicircular shape rather than a circular shape. That is, since only the tail can be twisted (rotated) when the rotary lever 120 is rotated independently, it may be possible to control the position of the caudal vein or caudal artery.

Meanwhile, the position control unit 300 is provided with a stopper unit 310 to control the degree of rotation of the rotation lever 120.

[Fixed part (200)]

As shown in FIGS. 1 and 2, the fixing part 200 may be composed of a jacket 210 and a bore system 220, or may be composed of a mold 230.

As shown in Figures 1 and 2, after putting the jacket 210 on the experimental animal, the experimental animal can be restrained to the seating plate 100 using the boa system 220. Here, the BOA system 220 is a fastening type mainly used in shoes, etc., where the laces are tightened according to the rotation of the fixing dial using a fixing dial and a string, and detailed description will be omitted. Alternatively, a string, etc. may be fastened to the jacket 210, and a portion of the string may be restrained to the seating plate 100, thereby restraining the experimental animal while wearing the jacket 210.

Meanwhile, as shown in FIG. 3, after the experimental animal is placed on the seating plate 100, the mold 230 may be fastened to the seating plate 100 while pressing the back of the experimental animal. The mold 230 may be made of a frame formed from a single mold, and it is preferable that the mold 230 and the fixing part 200 be easily fastened and fixed using a magnet or the like.

Due to the above detailed description, after placing the experimental animal on the upper part of the seating plate 100, the tail portion can be easily fixed separately for blood vessel sampling of the caudal vein or caudal artery or drug injection. In particular, not only can experimental animals be fixed very quickly and accurately, but also the tail can be easily fixed in a bent state of about 90 degrees, which will improve the repeatability and accuracy of the experimenter's experiment.

Meanwhile, the above detailed description should not be construed as restrictive in all respects and should be considered illustrative. The scope of the present invention should be determined by reasonable interpretation of the appended claims, and all changes within the equivalent scope of the present invention are included in the scope of the present invention.

100: Seating plate 200: Fixing part
300: Position control unit 400: Guide plate
500: Heating part 600: Support arm
700: Base

Claims (1)

A seating plate (100) on which an experimental animal with a tail is placed;
A fixing part 200 coupled to the seating plate 100 to restrain the experimental animal placed on the seating plate 100;
A position control unit 300 coupled to one side of the seating plate 100 to support the seating plate 100; and
The first guide plate 410 is coupled to the upper surface of the position control unit 300 and is fixed to the upper surface of one side of the position control unit 300, and the first guide groove 440 in which the tail is disposed is provided. It includes a second guide plate 420 that is spaced apart from the first guide plate 410 by a predetermined distance and is fixed to the upper surface of the other side of the position control unit 300, and at least one for restraining and determining the bending direction of the tail by the experimenter. guide plate 400;
A body holder and tail correction device for administration to the tail caudal vein of a laboratory rat, comprising:

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