WO2020213930A1 - Dispositif de collecte d'analyte, et procédé de collecte d'analyte ainsi que système d'inspection d'analyte l'utilisant - Google Patents

Dispositif de collecte d'analyte, et procédé de collecte d'analyte ainsi que système d'inspection d'analyte l'utilisant Download PDF

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Publication number
WO2020213930A1
WO2020213930A1 PCT/KR2020/005045 KR2020005045W WO2020213930A1 WO 2020213930 A1 WO2020213930 A1 WO 2020213930A1 KR 2020005045 W KR2020005045 W KR 2020005045W WO 2020213930 A1 WO2020213930 A1 WO 2020213930A1
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WIPO (PCT)
Prior art keywords
compartment
inner space
analyte
case
anallight
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PCT/KR2020/005045
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English (en)
Korean (ko)
Inventor
고건
이수봉
정연철
Original Assignee
(주)로고스바이오시스템스
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Application filed by (주)로고스바이오시스템스 filed Critical (주)로고스바이오시스템스
Priority to JP2021561652A priority Critical patent/JP7301416B2/ja
Priority to BR112021020767A priority patent/BR112021020767A2/pt
Priority to CA3137151A priority patent/CA3137151C/fr
Priority to AU2020258915A priority patent/AU2020258915B2/en
Priority to EP20791672.7A priority patent/EP3940386A4/fr
Priority to US17/440,106 priority patent/US20220187330A1/en
Priority to CN202080028476.XA priority patent/CN113711051A/zh
Publication of WO2020213930A1 publication Critical patent/WO2020213930A1/fr

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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/0098—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor involving analyte bound to insoluble magnetic carrier, e.g. using magnetic separation
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C12N15/1013—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by using magnetic beads
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/10—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0647—Handling flowable solids, e.g. microscopic beads, cells, particles
    • B01L2200/0668—Trapping microscopic beads
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
    • B01L2300/087—Multiple sequential chambers
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0475—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure
    • B01L2400/0478—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure pistons
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N2035/00178—Special arrangements of analysers
    • G01N2035/00306—Housings, cabinets, control panels (details)
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N2035/00465—Separating and mixing arrangements
    • G01N2035/00564—Handling or washing solid phase elements, e.g. beads

Definitions

  • the present invention relates to an analyte collection device, an analyte collection method using the same, and an analyte inspection system.
  • a sample collected from a human body or an animal's body is purified in a laboratory and a predetermined test is performed.
  • pretreatment and purification are performed on the sample through a chemical or physical method using a predetermined device, and the purified sample is finally collected as an analyte to perform a predetermined test.
  • an analyte collection device and method, and an analyte test system include a nucleic acid purification device, a method, and a purified nucleic acid test system.
  • Nucleic acid purification is an essential technology widely used in genetic engineering and molecular biology, and research as a pretreatment step for techniques such as Southern blot, Northern blot, and polymerase chain reaction (PCR). It is a very important technology for medical, industrial, and medical purposes.
  • the purification of such nucleic acids is traditionally performed through chemical and physical methods using ultrasound, heat, proteinase, alcohols, special reagents, etc., and the researcher performs the nucleic acid purification process using a pipette.
  • a method of purifying nucleic acids more conveniently using magnetic particles has recently been widely introduced. However, these methods have to be performed in a laboratory, and require a lot of time and labor, and are therefore limited in general use.
  • the nucleic acid purification process includes steps such as lysis of biological materials such as cells, binding of nucleic acid-magnetic particles, washing, and nucleic acid elution, and reagents suitable for the purpose of each step. And processing.
  • the analyte that has undergone such a purification process may be quantitatively collected and subjected to a predetermined necessary test.
  • the purified nucleic acid is transferred to a transparent amplification and detection tube, and amplification is performed using real-time PCR or similar techniques, and the presence of pathogenic nucleic acids is optically determined using a fluorescent label. Detection, and diagnosis of the disease is possible through this.
  • an analyte collection device for quantitatively collecting samples by purifying samples in this way should minimize the manpower required for the purification process, and It must be filled with a predetermined solution for, and must be small in size to ensure mobility.
  • POCT point-of-care testing
  • an analyte collection device for quantitatively collecting samples by purifying samples in this way should minimize the manpower required for the purification process, and It must be filled with a predetermined solution for, and must be small in size to ensure mobility.
  • POCT point-of-care testing
  • Embodiments of the present invention are for the purpose of purification or pre-treatment of samples using magnetic particles, the structure is simple, the cost is low, and the analysis light collection is implemented in a small size and enables efficient sample processing through an automated process. It is intended to provide an apparatus, an analyte collection method and an analyte inspection system using the same.
  • a case including an opening and an inner space; And at least one partition wall partitioning the inner space into a plurality of inner spaces, and including a piston inserted into the inner space through the opening of the case and provided to be reciprocally moved.
  • the inner space is formed to communicate with the outside at an end opposite to the side to which the piston is inserted, the discharge port through which the sample injected into the inner space is discharged from the case;
  • a blowback portion provided at an end opposite to the side into which the piston is inserted, and including a flow hole formed to communicate with the inner space at both ends thereof, may be provided.
  • the inner space is filled with a solution containing a magnetic material, and the sample injected into the inner space is subjected to a predetermined treatment by the solution filled in the inner space and discharged through the outlet as an analyte,
  • An analyte collection device may be provided.
  • At least one of the partition walls includes four partition walls, and the plurality of internal spaces includes a first compartment, a second compartment, a third compartment, and a fourth compartment sequentially partitioned by the four partition walls,
  • the magnetic material is disposed further away from the opening of the case from the first compartment to the fourth compartment, and the biomaterial contained in the sample is dissolved in the first compartment, so that at least a part of the analyte in the biomaterial is the magnetic material.
  • a solution for binding to is filled, and a solution for cleaning at least a portion of the analyte bound to the magnetic material is filled in the second compartment, and the third compartment is combined with the magnetic material.
  • a solution for allowing at least a portion of the analyte to be eluted from the magnetic material is filled, and the fourth compartment is formed adjacent to the third compartment and formed to contact the inner end of the case. Can be.
  • the solution filled in the first compartment contains at least one of a lysis/binding buffer and isopropyl alcohol (2-Propanol), and the solution filled in the second compartment is a washing buffer. ), and the solution to be filled in the third compartment may be provided with an analyte collection device including an elution buffer.
  • the piston includes a central pillar, at least one of the partition walls is composed of a plurality, the plurality of the partition walls are disposed to be spaced apart from each other, and formed to extend radially from the circumferential surface of the central pillar, the inner space
  • the analyte collection device may be provided, which is divided into a plurality of compartments by the partition walls, and in which at least some of the partitioned plurality of compartments are filled with different solutions.
  • the piston is attached to at least one of both surfaces of the partition wall provided perpendicular to the insertion direction of the piston, and the circumferential surface is provided closer to the inner wall surface surrounding the inner space of the case than the circumferential surface of the partition wall.
  • a sealing member provided to surround the circumferential surface of the partition wall and contacting the inner wall surface of the case, and an analyte collection device may be provided.
  • the case further includes a sample injection unit including an injection hole formed in the case to communicate the inner space and the outside of the case so that a sample may be injected, and the sample injection unit includes the injection hole.
  • An analyte collection device may be provided that further includes a stopper selectively covering the injection hole to seal the injection hole.
  • a cohesive groove is concave formed from the inner wall surface forming the inner space of the case, and a solution containing a magnetic material is filled in the inner space, and when a magnetic force is applied from the outside toward the coagulation groove, the magnetic material is An analyte collection device that can be aggregated in the agglomeration groove may be provided.
  • the analyte collected by the analyte collection device may include at least one or more of nucleic acids, proteins, vesicles, lipids, carbohydrates, cells, and substances that can be separated therefrom. .
  • a case including an opening and an inner space, and at least one partition wall partitioning the inner space into a plurality of inner spaces are included, and the inner space is provided in the inner space through the opening of the case.
  • An analyte collection device including a piston inserted and provided to be reciprocally moved; And a holder separably gripping the analyte collection device.
  • a plunger capable of translating the piston in the inner space by pushing or pulling the head of the piston; And a controller, wherein the plunger is controlled by the controller, and an analyte inspection system may be provided.
  • a solution containing a magnetic material is filled in the inner space, and a predetermined treatment is performed by the solution filled in the inner space for a sample to be introduced into the case, and the predetermined treatment includes a plurality of steps.
  • an analyte inspection system may be provided in which the plurality of steps are sequentially performed as the controller controls the driving of the plunger.
  • the case is formed so that the inner space communicates with the outside at an end opposite to the side where the piston is inserted, and the sample that has undergone a predetermined treatment by being introduced into the inner space can be discharged from the case as an analyte.
  • Outlet a blowback part provided at an end opposite to the side into which the piston is inserted, and including a flow hole formed at both ends to communicate with the inner space, and the controller pushes the piston toward the blowback part.
  • the analyte inspection system may be provided in which the analyte is discharged through the outlet by a blowback phenomenon.
  • a cohesive groove is concavely formed from an inner wall surface forming the inner space, and a magnetic force is selectively applied toward the cohesive groove so that the magnetic material can be coagulated in the cohesive groove, by the controller.
  • An analyte inspection system may be provided, further comprising a controlled agglomeration device.
  • an analyte inspection system may be provided.
  • the analyte collected by the analyte collection device may include at least one or more of nucleic acids, proteins, vesicles, lipids, carbohydrates, cells, and substances that can be separated therefrom. .
  • the analyte collection device comprises: a case including an opening and an inner space; And a piston including at least one partition wall dividing the inner space into a plurality of inner spaces, and provided to be reciprocally moved by being inserted into the inner space of the case, and inserting a sample into the inner space; And a step of sequentially proceeding a predetermined process including a plurality of steps while the piston moves in the inner space to collect the sample as an analyte, providing an analyte collection method using an analyte collection device Can be.
  • the case may include a discharge port formed so that the inner space communicates with the outside at an end opposite to the side where the piston is inserted, and through which the sample accommodated in the inner space can be discharged; And a blowback part provided at an end opposite to the side into which the piston is inserted, and including a flow hole formed at both ends to communicate with the inner space, and the piston moves within the inner space to process the predetermined
  • an analyte collection method using an analyte collection device may be provided.
  • a cohesive groove is concave formed from an inner wall surface forming the inner space of the case, and a magnetic material is contained in the solution filled in the inner space, and a magnetic force is applied to the coagulation groove to cause the magnetic material to be aggregated.
  • An analyte collection method using an analyte collection device may be provided, further comprising the step of agglomerating in the groove.
  • At least one of the partition walls includes four partition walls
  • the plurality of internal spaces includes a first compartment, a second compartment, a third compartment, and a fourth compartment sequentially partitioned by the four partition walls
  • the first compartment is formed closest to the opening of the case among the four compartments
  • the second compartment is formed adjacent to the first compartment with one of the compartments therebetween
  • the third compartment is It is formed adjacent to the second compartment with one of the partition walls therebetween
  • the fourth compartment is formed adjacent to the third compartment with one of the partition walls therebetween so as to contact the inner end of the case.
  • the step of sequentially performing the predetermined treatment includes dissolving the biological material contained in the sample by the solution filled in the first compartment, Combining at least a portion of the analyte with the magnetic material; Applying a magnetic force to the agglomeration groove to agglomerate the magnetic material combined with at least a portion of the analyte in the agglomeration groove, and then retreating the piston to place the second compartment above the agglomeration groove; Releasing the magnetic force applied to the cohesive groove, applying a magnetic force to the inner space to release the agglomerated state of the magnetic material aggregated in the cohesive groove, and allowing the magnetic material to float into the second compartment; Cleaning at least a portion of the analyte bound to the magnetic material by a solution filled in the second compartment; Applying a magnetic force to the agglomeration groove, after the magnetic material combined with at least a portion of the analyte is agglomerated in the
  • the solution filled in the first compartment contains at least one of a lysis/binding buffer and isopropyl alcohol (2-Propanol), and the solution filled in the second compartment is a washing buffer. ), and the solution filled in the third compartment includes an elution buffer, and an analyte collection method using an analyte collection device may be provided.
  • a lysis/binding buffer and isopropyl alcohol (2-Propanol) is a washing buffer.
  • the solution filled in the third compartment includes an elution buffer, and an analyte collection method using an analyte collection device may be provided.
  • the analyte collected by the analyte collection device includes at least one of nucleic acids, proteins, vesicles, lipids, carbohydrates, cells, and substances that can be separated from them.
  • a method can be provided.
  • the usability is high, and the user or a third party 2 It has the effect of preventing secondary infection.
  • FIG. 1 is a perspective view showing an analyte collection device according to an embodiment of the present invention.
  • FIG. 2 is an II-II cross-sectional view of FIG. 1.
  • FIG. 3 is a conceptual diagram illustrating an analyte inspection system including the analyte collection device of FIG. 1.
  • FIG. 4 is a flow chart illustrating a process of purifying and collecting an analyte using the analyte collection device of FIG. 1.
  • FIG. 5 is a longitudinal cross-sectional view showing an analyte collection device according to another embodiment of the present invention.
  • FIG. 6 is a conceptual diagram showing an analyte inspection system according to another embodiment of the present invention.
  • FIG. 7 is a conceptual diagram showing an analyte inspection system according to another embodiment of the present invention.
  • FIG. 8 and 9 are charts showing experimental results of polymerase chain reaction (PCR) of analytes collected using the analyte collection device of FIG. 1.
  • PCR polymerase chain reaction
  • the analyte collection device 10 may largely include a case 100 and a piston 200.
  • the case 100 and the piston 200 may be formed of, for example, any one of plastic, rubber, ceramic, inorganic compound, and metal, or a combination thereof.
  • the case 100 and the piston 200 for example, blow molding (Blow molding), compression molding (Compression molding), extrusion molding (Extrusion molding), injection molding (Injection molding), laminating (Laminating), Reaction injection molding, Matrix molding, Rotational molding, Spin casting, Transfer molding, Thermoforming, 3D printing, etc. It can be manufactured through the process of.
  • the case 100 and the piston 200 may be mass-produced by an already equipped automated facility, and for example, may be produced for single use.
  • the case 100 and the piston 200 may be separately manufactured and assembled to be provided.
  • the case 100 has a space 102 formed therein so that a sample can be injected, and the piston 200 may be inserted into the inner space 102 to be mutually assembled.
  • the inner space 102 of the case 100 may be provided in a shape with one side open, and is formed in a shape corresponding to the piston 200, so that the piston 200 reciprocates while being inserted into the inner space 102 It may be provided to enable movement.
  • the inner space 102 of the case 100 may be divided into a plurality of compartments by the partition wall 230 of the piston 200 to be described later.
  • the inner space 102 of the case 100 may be divided into four compartments 102a, 102b, 102c, and 102d, but the spirit of the present invention is not limited thereto.
  • the sample injected into the inner space 102 may be made of a liquid, solid, or mixture thereof including some or all of cells, viruses, tissues, exosomes, proteins, nucleic acids, antigens, and antibodies. It may be a sample taken from the human body.
  • the sample injected into the internal space 102 is a sample collected from the human body, for example, the intracellular nucleic acid present in the sample may be purified using the analyte collection device 10.
  • the inner space 102 may be filled with a solution containing a magnetic material, and different solutions may be filled in the plurality of compartments.
  • the first compartment 102a is a piston among the four compartments 102a, 102b, 102c, and 102d. It is formed closest to the opening of the case 100 formed so that the 200 can be inserted, and the biomaterial contained in the sample is dissolved in the first compartment 102a, so that at least a part of the analyte in the biomaterial is a magnetic material. It may be filled with a solution to allow it to bind with.
  • the analyte collected through the analyte collection device 10 is a sample of nucleic acids, proteins, vesicles (Exosome, etc.), lipids, carbohydrates, cells (blood cells, immune cells, tumor cells, pathogenic microorganisms, etc.).
  • the biomaterial contained in itself or a material that can be separated from the biological material by a physical and/or chemical method may be included.
  • the intracellular nucleic acid present in the sample is purified using the analyte collection device 10
  • the analyte collected through the analyte collection device 10 may contain purified nucleic acid. have.
  • the solution filled in the first compartment 102a may include, for example, at least one of a Lysis/binding Buffer and an isopropyl alcohol (2-Propanol), and more specifically, magnetic Magnetic nano/micro particles, salts (ex. Tris-HCl), chelating agent (ex. Ethylenediaminetetraacetic acid (EDTA)), surfactant/detergent (ex. Sodium dodecyl sulfate (SDS) ), Triton X-100), reducing agent (reductant; ex. Dithiothreitol (DTT)), chaotropic agent (ex. Guanidine thiocyanate), enzyme (ex. Proteinase K), alcohol (ex. 2) -Propanol) and purified water (Distilled water) may be provided as a solution containing some or all of.
  • a Lysis/binding Buffer and an isopropyl alcohol (2-Propanol) and more specifically, magnetic Magnetic nano/micro particles, salt
  • the second compartment 102b is formed adjacent to the first compartment 102a with one of the plurality of partitions 230 interposed therebetween, and in the second compartment 102b, an analyte of an analyte bonded to a magnetic material A solution that allows at least some of the cleaning to proceed may be filled.
  • the solution filled in the second compartment 102b may include, for example, a washing buffer, and more specifically, Diethyl pyrocarbonate (DEPC), Sodium citrate tribasic dehydrate, and alcohol (ex. Ethanol, 2) -propanol) and purified water (Distilled water) may be provided as a solution containing some or all of.
  • DEPC Diethyl pyrocarbonate
  • Sodium citrate tribasic dehydrate e.g., 2, 2, -propanol
  • purified water disilled water
  • the third compartment 102c is formed adjacent to the second compartment 102b with one of the plurality of partitions 230 interposed therebetween, and in the third compartment 102c, an analyte of an analyte bonded to a magnetic material May be filled with a solution such that at least a portion of it elutes from the magnetic material,
  • the solution filled in the third compartment 102c may include, for example, an elution buffer, and more specifically, salts (ex. Tris-HCl), a chelating agent (ex. Ethylenediaminetetraacetic acid (EDTA)), diethyl pyrocarbonate (DEPC), and distilled water may be provided as a solution containing some or all of them.
  • salts ex. Tris-HCl
  • EDTA Ethylenediaminetetraacetic acid
  • DEPC diethyl pyrocarbonate
  • distilled water distilled water
  • the fourth compartment 102d is formed to be adjacent to the third compartment 102c with one of the plurality of partitions 230 interposed therebetween, and is formed to be in contact with the inner end positioned opposite the opening of the case 100. I can.
  • the fourth compartment 102d may be provided in a state filled with gas such as air.
  • the case 100 may include a sample injection unit 110, a cohesive groove 120, a blowback unit 130, an outlet 140, a mounting unit 150, and a wing unit 160.
  • the sample injection unit 110 may include an injection hole 112 formed in the case 100 to communicate the inner space 102 and the outside of the case 100 so that a sample may be injected, and the injection hole A stopper 114 may be further included to selectively cover 112 to seal the injection hole 112.
  • the injection hole 112 may be provided in a shape that has a wide upper surface and narrows downward, and may be formed in a funnel shape as an example, but the idea of the present invention will be limitedly interpreted by the shape of the injection hole 112 no.
  • the injection hole 112 may be concavely formed with respect to the upper surface of the case 100 so that the inner space 102 and the lower end communicate with each other.
  • this is only an example, and the injection hole 112 may be formed on a side surface or a bottom surface other than the upper surface of the case 100.
  • each of the compartments 102a, 102b, 102c, and 102d may be sequentially communicated with the lower end.
  • the sample is injected through the injection hole 112, it can be injected while being in communication with the first compartment 102a, and the first step in the purification process will proceed immediately after the sample is injected into the first compartment 102a. I can.
  • the stopper 114 may be made of a material having elasticity such as rubber, for example, and the lower portion is formed to correspond to the shape of the injection hole 112 so that the injection hole 112 is formed when the lower end is inserted into the injection hole 112. It can be provided so that it can be completely covered and sealed. When not in use, such a stopper 114 can seal the injection hole 112 to prevent foreign substances from penetrating into the inner space 102, and the stopper 114 is in order to inject a sample into the injection hole 112 Separated from the injection hole 112, the injection hole 112 may be opened. After the sample is injected through the injection hole 112, the stopper 114 may be coupled again to seal the injection hole 112. Accordingly, it is possible to prevent external foreign substances from penetrating into the inner space 102 before treatment as well as during a treatment process.
  • the cohesive groove 120 is concave formed from the inner wall surface forming the inner space 102 of the case 100, and when a magnetic force is applied from the outside toward the cohesive groove 120, the magnetic material filled in the inner space 102 is It may be agglomerated in the agglomeration groove 120. At this time, since the dissolution and bonding action of the sample occurs in the first compartment 102a, when the magnetic material and other biological material are combined, the magnetic material and the material bonded thereto may be aggregated in the agglomeration groove 120.
  • the cohesive groove 120 may be formed on the inner wall surface of the case 100 so as not to interfere with the injection hole 112, and in this embodiment, a case formed on the bottom surface of the inner space 102 of the case 100 Although shown as an example, the spirit of the present invention is not limited thereto.
  • the cohesive groove 120 may be formed on the side or upper surface of the inner space 102.
  • the cohesive groove 120 may be formed as a hemispherical groove, but due to the shape of the cohesive groove 120, the idea of the present invention is not limitedly interpreted, and in some cases, other shapes such as a funnel shape, a hexahedral shape, etc. It may be formed as.
  • the cohesive groove 120 may be formed on the same line as the injection hole 112.
  • at least the first compartment 102a may be formed at a position within a range in which the cohesive groove 120 and the injection hole 112 can communicate simultaneously. Accordingly, it is possible for the sample injected through the injection hole 112 to be directly accommodated and aggregated into the aggregation groove 120 without further movement of the piston 200.
  • this is only an example, and even if the cohesive groove 120 is formed at a position that cannot communicate with the injection hole 112 and any one of the compartments 102a, 102b, 102c, 102d at the same time, the piston 200 Aggregation of the sample may be made possible by additional movement.
  • the blowback part 130 is provided at an end opposite to the side into which the piston 200 is inserted, and includes a flow hole 132 formed so that both ends communicate with the inner space. Further, the blowback part 130 may be formed on the upper surface of the case 100, but the spirit of the present invention is not limited thereto, and may be formed on the side or the bottom surface of the case 100.
  • gas such as air existing in the fourth compartment 102d is blown back through the flow hole 132. Accordingly, the purified analyte existing in the third compartment 102c may be discharged through the outlet 140 and collected into a collection container (X, see FIG. 3 ).
  • the flow hole 132 includes a flow hole inlet 1322, a bridge 1324 and a flow hole outlet 1326. Both the flow hole inlet 1322 and the flow hole outlet 1326 are formed so that one end communicates with the inner space 102, and each other end is connected through a bridge 1324, so that the flow hole 132 as a whole is' It can be formed as a U'-shaped channel. In this case, the flow hole inlet 1322 may be formed closer to the end opposite to the opening of the inner space 102 than the flow hole outlet 1326.
  • gas such as air in the fourth compartment 102d is introduced into the flow hole inlet 1322 by pressure, and the bridge 1324 And after passing through the flow hole outlet 1326, it may flow into the third compartment 102c adjacent to the fourth compartment 102d. Due to the pressure of the introduced gas, the analyte accommodated in the third compartment 102c may be pushed through the outlet 140 and discharged from the case 100. The analyte discharged in this way may be collected into the inside of the collection container X coupled to the container coupling protrusion 142 to be described later.
  • the user can finely adjust the amount of gas blown back through the flow hole 132 by adjusting the degree to which the piston 200 is pressurized.
  • the amount of the analyte discharged and collected through the discharge port 140 can be finely adjusted.
  • the analyte collecting device 10 according to the present embodiment It can be particularly useful when performing tests where quantitative collection is very important.
  • the flow hole 132 of the blowback part 130 may be formed in an open shape so that the upper surface of the bridge 1324 communicates with the outside. Accordingly, a cover 134 for selectively sealing the opening surface of the bridge 1324 may be additionally provided.
  • the cover 134 may be made of a material having elasticity such as rubber, for example, and the lower end is formed to correspond to the shape of the bridge 1324 so that when the lower end of the cover 134 is inserted into the bridge 1324, the bridge It may be provided to be able to seal while completely covering the opening of 1324.
  • Such a cover 134 can seal the flow hole 132 to prevent external foreign matters from penetrating into the inner space 102, and thereby, the external foreign matters not only before treatment, but also during the treatment process. 102) can be prevented.
  • the flow hole 132 of the blowback part 130 is formed in an open shape to communicate with the outside, and the opening of the flow hole 132 is sealed by the cover 134 as an example.
  • the idea of the present invention is not limited thereto.
  • the flow hole 132 may be formed in a shape in which the bridge 1324 is not opened and communicates only with the inner space 102 without itself communicating with the outside.
  • the discharge port 140 is formed to communicate with the outside at the opposite end of the opening into which the piston 200 is inserted, and formed so that a sample that has undergone a predetermined treatment in the inner space 102 can be discharged from the case 100 as an analyte. do.
  • the discharge port 140 is formed through one side of the case 100, and may be formed at a position opposite to the blowback part 130 as shown in FIG. 2. However, this is only an example, and the discharge port 140 may be formed at a position not facing the blowback part 130.
  • the outlet 140 is formed on the bottom surface of the inner space 102 is illustrated as an example, but the spirit of the present invention is not limited thereto, and is formed on the side or upper surface of the inner space 102 It is also possible.
  • the analyte when the analyte is discharged through the discharge port 140, the force due to gravity is not received, but the analyte can be collected by the pressure caused by the blowback phenomenon.
  • a container coupling protrusion 142 may be formed at a portion of the case 100 where the outlet 140 is formed.
  • the container coupling protrusion 142 may be formed to protrude from the outer surface of the case 100, and may be formed in a shape extending the outlet 140 to the outside of the case 100.
  • the container coupling protrusion 142 may be formed in a shape that can be fastened with the collection container X, and the container coupling protrusion 142 and the collection container X may be fastened to each other in a manner such as mold fitting or screwing.
  • the collection container (X) may be made of a soft plastic material, but the spirit of the present invention is not limited thereto.
  • the mounting part 150 may be provided on the bottom of the case 100 and held by a holder 20 to be described later so that the analyte collection device 10 may be mounted.
  • wing portions 160 may be formed on both sides of the mounting portion 150.
  • the wing portion 160 may be formed in a shape protruding over the entire side width of the case 100, and when the mounting portion 150 is gripped by the holder 20, it supports both sides of the holder 20 By doing so, the case 100 can be stably gripped and fixed to the holder 20 due to interference between the holder 20 and the wing unit 160.
  • the side surface of the wing unit 160 may be attached to a label (not shown) or may be used as a space for taking a predetermined writing. This makes it possible to systematically manage the analyte collection device 10.
  • the piston 200 is provided to be inserted into the inner space 102 through an opening formed in the case 100, and is provided to be reciprocated within the inner space 102.
  • the piston 200 includes at least one partition wall 230 that partitions the inner space 102.
  • the piston 200 may further include a central pillar 210, a piston head 220, a flange 240, and a sealing member 250.
  • the central pillar 210 may be provided in a cylindrical shape, for example, and is provided to connect the piston head 220 and the partition wall 230.
  • the plurality of partition walls 230 may be connected, and the thickness of the portion connecting the piston head 220 and the partition wall 230 and the portion connecting the plurality of partition walls 230 may be formed differently.
  • the thickness of the portion connecting the plurality of partition walls 230 may be set to be thinner than the thickness of the portion connecting the piston head 220 and the partition wall 230, and accordingly, the central pillar 210
  • the space of each compartment 102a, 102b, 102c, 102d may be occupied only in a limited way.
  • the central pillar 210 is provided with the same thickness as a whole, or connects the plurality of partition walls 230 to the thickness of the portion connecting the piston head 220 and the partition wall 230. It is also possible to set the thickness of the part to be thicker.
  • the piston head 220 is connected to the end of the central column 210 and may be provided to be selectively clamped by a clamp 510 of the plunger 50 to be described later.
  • the piston head 220 may be provided in a disk shape having a larger radius than the central pillar 210, and may be provided in a flange shape with respect to the central pillar 210.
  • the partition wall 230 is radially extended from the circumferential surface of the central pillar 210 so that a plurality of the partition walls 230 are disposed to be spaced apart from each other.
  • the partition wall 230 is provided in four to divide the inner space 102 into a total of four compartments 102a, 102b, 102c, and 102d, but this is not limited to the spirit of the present invention, and if necessary It can be provided in any number other than four.
  • an analyte collection device 10' according to another embodiment of the present invention is provided.
  • the number of the partition walls 230 is two, and accordingly, the number of the compartments 102a and 102b may also be provided in two.
  • the analyte collection device 10' having such a configuration can only be processed by a single solution in the inner space 102, and a plurality of devices are required for a processing process that requires a plurality of steps. It has the advantage of being able to use it easily when only a single treatment is needed, the size of the product is miniaturized, the unit cost is lowered.
  • the idea of the present invention is not limited by the number of partitions and compartments, and in some cases, three or more partitions and compartments may be formed.
  • the flange 240 is attached to at least one of both sides of the partition wall 230, and the circumferential surface forms the inner space 102 of the case 100 than the circumferential surface of the partition wall 230. It is provided closer to the inner wall surface.
  • the radius of the flange 240 may be provided larger than the radius of the partition wall 230. Due to the shape of the flange 240, when two flanges 240 are attached to both sides of the partition wall 230, respectively, a space is formed along the circumference of the partition wall 230 between the two flanges 240. I can.
  • the sealing member 250 may be provided in the space thus formed.
  • the sealing member 250 is provided to surround the circumferential surface of the partition wall 230 and is in contact with the inner wall surface of the case 100.
  • the sealing member 250 may be an O-ring made of a material such as rubber.
  • the gap between the partition wall 230 and the inner wall surface of the case 100 may be sealed by the sealing member 250, and the material contained in each of the compartments 102a, 102b, 102c, and 102d is prevented from leaking from the corresponding compartment. Can be.
  • the sealing member 250 is not separated from the circumferential surface of the partition wall 230 A state disposed between the flanges 240 may be maintained.
  • a process for collecting a sample as an analyte through processing such as purification by the analyte collection device 10 having the configuration as described above, and a process in which a predetermined inspection of the collected analyte is performed can be performed manually. In addition, it is also possible to be performed automatically by the analyte inspection system 1. Compared to manual operation, when the analyte inspection system 1 is used, more precise control is possible, so that the analyte collection and inspection can be performed more systematically.
  • an analyte inspection system 1 according to an embodiment of the present invention will be described.
  • the analyte inspection system 1 processes not only a predetermined inspection of the analyte collected through the analyte collection device 10, but also the purification of the analyte. It can be configured to be able to perform and collection together. However, in some cases, the analyte inspection system 1 may be configured so that only processing and collection such as purification of the analyte can be performed.
  • Such an analyte inspection system 1 may include a holder 20, a flocculation device 30, a flocculation release device 40, a plunger 50, and a controller 60.
  • the holder 20 is provided to removably grip the analyte collection device 10.
  • the upper surface of the holder 20 may have a shape that can be fastened with the mounting portion 150 of the analyte collection device 10, and the holder 20 has a width that can be inserted into the space between the wing portions 160 on both sides. It can have a shape. Accordingly, a process for collecting the analyte by the analyte collection device 10 may be performed in a state in which the analyte collection device 10 is mounted by the holder 20.
  • the agglomeration device 30 selectively applies a magnetic force toward the agglomeration groove 120 of the analyte collection device 10 so that the magnetic material in the solution accommodated in the inner space 102 and the material bound thereto are aggregated in the agglomeration groove 120 It can be provided so that it can be.
  • the agglomeration device 30 may be controlled by a controller 60 to be described later, and may be provided as a member such as an electromagnet capable of generating magnetic force by receiving power. Further, the agglomeration device 30 may be provided in the holder 20, and may be disposed at a position close to the agglomeration groove 120 when the analyte collection device 10 is mounted on the holder 20.
  • the deagglomeration device 40 may be provided to selectively apply a magnetic force to the inner space 102.
  • the deagglomeration device 40 may be controlled by the controller 60 and may be provided as a member such as an electromagnet capable of generating magnetic force by receiving power.
  • the deagglomeration device 40 may be disposed adjacent to the side or upper surface of the case 100 and may be driven to apply magnetic force to the inner space 102 of the case 100 by receiving power from the outside. .
  • the controller 60 controls the deagglomeration device 40 in a state in which the magnetic material is agglomerated in the agglomeration groove 120, so that the magnetism aggregated in the agglomeration groove 120 by applying a magnetic force to the inner space 102
  • the agglomerated state of the material and the material bound thereto is released, and the magnetic material and the material bound thereto may be floated back into the inner space 102.
  • the plunger 50 is provided to push or pull the piston head 220 to move the piston 200 in translation within the inner space 102.
  • the plunger 50 may include a clamp 510 capable of selectively gripping the piston head 220.
  • the clamp 510 may have a shape corresponding to the shape of the piston head 220 and is provided to selectively grip the piston head 220.
  • the controller 60 may be provided to control each element of the analyte inspection system 1. Specifically, the controller 60 may be provided to control at least one or more of the holder 20, the agglomeration device 30, the agglomeration release device 40, and the plunger 50.
  • the controller 60 may be made of, for example, a small built-in computer, and may include a program, a memory, a data processing unit made of a CPU, and the like. Such a program may include an algorithm for controlling at least one of the holder 20, the agglomeration device 30, the agglomeration disengagement device 40, and the plunger 50.
  • a program may be stored in a memory such as a computer storage medium, such as a flexible disk, a compact disk, a hard disk, or a magneto-optical disk (MO), and installed in the controller 60.
  • a predetermined process performed in the analyte collection device 10 by the analyte inspection system 1 includes a plurality of steps, and as the controller 60 drives and controls the plunger 50, such A plurality of steps may be performed sequentially.
  • analyte collection device 10 is described as an example in which a sample for polymerase chain reaction (PCR) is included as a purified nucleic acid, but this limits the scope of the present invention.
  • PCR polymerase chain reaction
  • the analyte collection device and the analyte inspection system according to embodiments of the present invention may be used for collecting other types of analytes.
  • a sample may be injected into the inner space 102 of the analyte collection device 10 (FIG. 4(a)).
  • the sample injected into the inner space 102 may be made of a liquid, solid, or mixture thereof containing some or all of cells, viruses, tissues, exosomes, proteins, nucleic acids, antigens, and antibodies, For example, it may be a sample taken from a human body.
  • a predetermined process including a plurality of steps may be sequentially performed.
  • a step in which a predetermined process is sequentially performed will be exemplarily described as follows.
  • the biological material contained in the sample is dissolved by the solution filled in the first compartment 102a, so that the nucleic acid in the biological material may be combined with the magnetic material (FIG. 4(b)).
  • the controller 60 controls the agglomeration device 30 to apply a magnetic force to the agglomeration groove 120, and a magnetic material combined with a nucleic acid may be aggregated in the agglomeration groove 120 by magnetic force (Fig. 4(c)). ).
  • the controller 60 may retract the piston 200 so that the second compartment 102b is disposed above the aggregation groove 120 (Fig. 4(d)).
  • the controller 60 stops driving the coagulation device 30 to release the magnetic force applied to the coagulation groove 120, and drives the coagulation release device 40 to Magnetic force can be applied to 102 (Fig. 4(e)). Accordingly, the aggregation state of the magnetic material combined with the nucleic acid aggregated in the aggregation groove 120 is released, and the magnetic material combined with the nucleic acid may be suspended into the inner space of the case. Thereafter, the nucleic acid bound to the magnetic material may be washed by the solution filled in the second compartment 102b.
  • the controller 60 drives the agglomeration device 30 again to apply a magnetic force to the agglomeration groove 120 to transfer the magnetic material bound to the nucleic acid to the agglomeration groove 120 ) Can be aggregated (Fig. 4(f)). Thereafter, the controller 60 drives the plunger 50 to retract the piston 200 so that the third compartment 102c may be disposed above the cohesive groove 120 (FIG. 4(g)).
  • the controller 60 stops driving the coagulation device 30 to release the magnetic force applied to the coagulation groove 120, and drives the coagulation release device 40 to Magnetic force can be applied to 102 (Fig. 4(h)). Accordingly, the agglomeration state of the magnetic material combined with the nucleic acid aggregated in the agglomeration groove 120 is released, and the magnetic material combined with the nucleic acid may be suspended into the inner space of the case 100. Thereafter, a step of eluting the nucleic acid bound to the magnetic material from the magnetic material by the solution filled in the third compartment 102c may be performed.
  • the controller 60 drives the agglomeration device 30 again to apply a magnetic force to the agglomeration groove 120 to transfer the magnetic material separated from the nucleic acid into the agglomeration groove ( 120) can be aggregated (Fig. 4(i)). Thereafter, the controller 60 pushes and moves the piston 200 from the inner space 102 toward the end of the inner space 102, thereby moving the third compartment 102c containing the eluted nucleic acid into the blowback part 130 ) And pressurized toward the blowback unit 130 may be discharged as an analyte through the discharge port 140 formed in the case 100 (FIG. 4(j)).
  • the container coupling protrusion 142 may be in a state in which the collection container X is fastened, and the analyte discharged through the outlet 140 is collected in the collection container X, and predetermined polymerase chain reaction (PCR), etc. Can be used in the inspection procedure of.
  • PCR polymerase chain reaction
  • the analyte inspection system 1 may be configured to control a single system from one controller 60, as shown in FIG. 3, but as shown in FIG. 6, control a plurality of systems It is also possible to be configured to do.
  • FIG. 5 when only two compartments are provided, such as the analyte collection device 10', and one compartment is filled with a solution for a single treatment, it must proceed through a plurality of steps.
  • a plurality of analyte collection devices 10 may be required. In this way, when it is necessary to operate a plurality of the analyte collection devices 10 in one process, the analyte inspection system 1'as shown in FIG. 6 may be used.
  • the analyte inspection system 1' is provided with a plurality of analyte collection devices 10, and a holder 20, an agglomeration device ( 30), a deagglomeration device 40 and a plunger 50 are provided, and a transfer line 70 connecting each of the analyte collecting devices 10 may be additionally provided.
  • the transfer line 70 may be provided to connect the outlet 140 of one of the analyte collection devices 10 and the injection hole 112 of the other adjacent analyte collection device 10. Accordingly, the analyte processed by the analyte collection device 10 at the front end is injected into the analyte collection device 10 at the rear end through the transfer line 70 and subsequent processing may be performed.
  • a member such as a pump or valve for controlling the transfer of fluid may be additionally provided in the transfer line 70.
  • the transfer line 70 may be detachably fastened to the analyte collection device 10, whereby an operation such as fastening to the analyte collection device 10 or changing its arrangement is possible as necessary.
  • this is only an example, and the transfer line 70 is omitted, and the analyte discharged from the analyte collection device 10 at the front end may be manually transferred to the analyte collection device 10 at the rear end. Do.
  • the controller 60 may include a plunger module 610, a coagulation release module 620 and a coagulation module 630.
  • the plunger module 610 may be connected to each plunger 50 and may be provided to independently control the plungers 50.
  • the deagglomeration module 620 may be provided to be connected to each deagglomeration device 40 to independently control the deagglomeration devices 40.
  • the agglomeration module 630 may be provided to be connected to each agglomeration device 30 to independently control each agglomeration device (30).
  • the plunger module 610, the deagglomeration module 620, and the agglomeration module 630 may be provided as a chipset module provided in the controller 60 or as independent algorithms stored in one chipset.
  • the configuration of the controller 60 of the analyte inspection system can be variously modified.
  • a configuration as shown in FIG. 7 is also possible.
  • an analyte inspection system 1'' according to another embodiment of the present invention will be described with reference to FIG. 7.
  • the analyte inspection system 1 ′′ is mainly described, and the same content is described above.
  • One analyte inspection system (1') is used.
  • the controller 60 of the analyte inspection system 1 ′′ includes an analyte collection device 10, a holder 20, an agglomeration device 30, a deagglomeration device 40, and a plunger 50.
  • Each module may have a configuration corresponding to each system in which each of the) is included one by one.
  • the controller ( 60) may include a first module 602, a second module 604 and a third module 606 that independently control each of the systems. Accordingly, there is an advantage that it is possible to easily change the number of each sub-system included in the entire system by removing some of the modules in the controller 60 or adding a new module.
  • an analyte collection including a first compartment 102a, a second compartment 102b, a third compartment 102c, and a fourth compartment 102d
  • An apparatus was prepared, and the experiment was conducted by setting the volumes of the first to third compartments 102a, 102b, and 102c to 750, 750, and 550 ⁇ L, respectively.
  • the Influenza A H1N1 (Human, strain: KUMC-76) cDNA was transferred and the polymerase chain reaction (PCR) of the transfer result was performed using the analyte collection device according to the embodiments of the present invention. And the results were measured.
  • the equipment used for the PCR was ThermoFisher Scientific (Applied Biosystems) QuantStudioTM 3, and the reagent was the same manufacturer's PowerUpTM SYBRTM Green Master Mix. Primer information used is as follows.
  • Inf.A_F GACCRATCCTGTCACCTCTGAC (22 mer, 10 ⁇ M)
  • Inf.A_R AGGGCATTYTGGACAAAKCGTCTA (24 mer, 10 ⁇ M)
  • the PCR reaction volume was 20 ⁇ L, including 10 ⁇ L of PowerUpTM SYBRTM Green Master Mix, 1 ⁇ L of the aforementioned primers, and 8 ⁇ L of samples for each experiment.
  • the PCR procedure consisted of a total of three stages. First, as a hold stage, 50 °C/2 min., 95 °C/10 min. After progressing under conditions, the Cycling Stage is 95 °C/15 sec., 60 °C/60 sec. 40 cycles were carried out. Finally, the Melt Curve Stage is 95 °C/15 sec., 60 °C/1 min. After that, the result was measured by increasing 0.15 °C in 1 second.
  • Influenza A H1N1 Human, strain: KUMC-76
  • cDNA corresponding to the sample was prepared by Qiagen's QuantiNovaTM Reverse Transcription Kit.
  • a solution required for washing was pre-injected into the second compartment 102b, and 750 ⁇ L of Washing Buffer2 from Dynabeads® SILANE viral NA kit (ThermoFisher Scientific) was injected.
  • a solution required for nucleic acid elution was pre-injected into the third compartment 102c, and the cartridges shown in FIGS. 8 and 9, E.B.
  • 150 ⁇ L of Elution Buffer and 400 ⁇ L of DW of Dynabeads® SILANE viral NA kit (ThermoFisher Scientific) were injected, and 550 ⁇ L of DW was injected in the cartridge and H2O experiment shown in FIGS. 8 and 9.
  • the same sample was passed through the same solutions as the solution injected into the first compartment 102a and the second compartment 102b, and at the end, 150 ⁇ L of Elution Buffer and 400 ⁇ L of DW were passed through the injected solution.
  • the experimenter performed manually using a pipette (pipetting in FIGS. 8 and 9, EB).
  • 8 ⁇ L of analyte stock solution, 1/10 dilution, and 1/100 dilution were injected into individual PCR reaction vessels to perform PCR reaction.
  • Influenza A H1N1 cDNA injected in each experiment is 5.5 ⁇ L, and the volume of the third compartment 102c is 550 ⁇ L, so when 100% of the analyte is transferred, it is equivalent to 1/100 of the standard dilution.
  • the measured threshold cycle (Ct) values were all similar to the Ct values of the 1/100 dilution of the control and standard of this experiment, and it was confirmed that the dilutions also showed the same tendency. . Since the Ct value is a value that disproves the initial cDNA before amplification, it was determined that the analyte collection through the analyte collection apparatus, system and method according to the embodiments of the present invention is effective.
  • the structure of the device and system is simple, so the cost is low, and it can be implemented in a small size and Through the process, efficient sample processing is possible, and there is an effect that a constant analyte yield can be achieved for each round regardless of the user's skill level.

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Abstract

La présente invention concerne un dispositif de collecte d'analyte, et un procédé de collecte d'analyte ainsi qu'un système d'inspection d'analyte l'utilisant. Spécifiquement, un mode de réalisation de la présente invention peut fournir un dispositif de collecte d'analyte, et un procédé de collecte d'analyse ainsi qu'un système d'inspection d'analyte l'utilisant, ledit dispositif de collecte d'analyte comprenant : un boîtier comprenant une ouverture et un espace interne; et un piston qui comprend au moins une paroi de séparation pour diviser l'espace interne en de multiples espaces internes et est destiné à être inséré dans l'espace interne à travers l'ouverture du boîtier afin de pouvoir effectuer un mouvement de va-et-vient à l'intérieur de celui-ci.
PCT/KR2020/005045 2019-04-16 2020-04-14 Dispositif de collecte d'analyte, et procédé de collecte d'analyte ainsi que système d'inspection d'analyte l'utilisant WO2020213930A1 (fr)

Priority Applications (7)

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JP2021561652A JP7301416B2 (ja) 2019-04-16 2020-04-14 アナライト収集装置、それを用いたアナライト収集方法及びアナライト検査システム
BR112021020767A BR112021020767A2 (pt) 2019-04-16 2020-04-14 Dispositivo de coleta de analito, e método de coleta de analito e sistema de inspeção de analito usando o mesmo
CA3137151A CA3137151C (fr) 2019-04-16 2020-04-14 Dispositif de collecte d'analyte, et procede de collecte d'analyte ainsi que systeme d'inspection d'analyte l'utilisant
AU2020258915A AU2020258915B2 (en) 2019-04-16 2020-04-14 Analyte collecting device, and analyte collecting method and analyte inspection system using same
EP20791672.7A EP3940386A4 (fr) 2019-04-16 2020-04-14 Dispositif de collecte d'analyte, et procédé de collecte d'analyte ainsi que système d'inspection d'analyte l'utilisant
US17/440,106 US20220187330A1 (en) 2019-04-16 2020-04-14 Analyte collecting device, and analyte collecting method and analyte inspection system using same
CN202080028476.XA CN113711051A (zh) 2019-04-16 2020-04-14 分析物收集装置、利用其的分析物收集方法及分析物检查系统

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CN116953274A (zh) * 2022-04-19 2023-10-27 宋世平 一种按序加样装置及自动加样系统
CN117309496B (zh) * 2023-10-28 2024-03-01 河海大学 一种河道泥沙采样监测设备

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AU2020258915B2 (en) 2023-09-28
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KR20200121681A (ko) 2020-10-26
CN113711051A (zh) 2021-11-26
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