WO2020177442A1 - 一种不结球白菜种子引发剂及其应用方法 - Google Patents

一种不结球白菜种子引发剂及其应用方法 Download PDF

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WO2020177442A1
WO2020177442A1 PCT/CN2019/125941 CN2019125941W WO2020177442A1 WO 2020177442 A1 WO2020177442 A1 WO 2020177442A1 CN 2019125941 W CN2019125941 W CN 2019125941W WO 2020177442 A1 WO2020177442 A1 WO 2020177442A1
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seeds
germination
ascorbic acid
seed
heading
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French (fr)
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侯喜林
颜晓艺
刘高峰
张昌伟
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南京农业大学
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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01C—PLANTING; SOWING; FERTILISING
    • A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
    • A01N43/04—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
    • A01N43/06—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom five-membered rings
    • A01N43/08—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom five-membered rings with oxygen as the ring hetero atom
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
    • A01N43/04—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
    • A01N43/06—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom five-membered rings
    • A01N43/12—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom five-membered rings condensed with a carbocyclic ring
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N45/00—Biocides, pest repellants or attractants, or plant growth regulators, containing compounds having three or more carbocyclic rings condensed among themselves, at least one ring not being a six-membered ring
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N59/00—Biocides, pest repellants or attractants, or plant growth regulators containing elements or inorganic compounds
    • A01N59/06—Aluminium; Calcium; Magnesium; Compounds thereof

Definitions

  • the invention relates to the technical field of seed initiation, in particular to a non-heading cabbage seed initiator and an application method thereof.
  • Seed priming is a pre-sowing seed treatment technology that allows seeds to slowly absorb water under controlled conditions to prepare physiologically in advance for germination. It is an effective way to improve seed vigor. Seed priming technology has been successfully applied to vegetable crops, agricultural crops, economic crops, ornamental plants, forest trees and other plant species, including eggplant, pepper, tomato, cucumber, cantaloupe, melon, watermelon, cabbage, carrot, Celery, spinach, green onions, onions and other vegetables; barley, wheat, corn, rice and sorghum and other food crops; and snow lotus, verbena, petunia and pansy and other ornamental plants. A good germination effect was obtained through the priming treatment, the neat rate and resistance of the seedlings were improved, and a good foundation was laid for its later growth and development.
  • Non-heading Chinese cabbage (Brassica campestris ssp.chinensis Makino) belongs to the Brassica genus Brassica subspecies of the Brassicaceae family. It is commonly known as green cabbage, pakchoi, rape, etc. It is an important vegetable crop native to my country. Non-heading cabbage has a fresh and tender texture and rich nutrition. Non-heading cabbage is mostly cultivated by direct seeding. The seed particles are small, and there are actual production problems such as large seeding rate, low seedling rate, and poor seedling quality. Therefore, improving seed vigor and seedling quality has an impact on the yield and quality of non-heading cabbage. Has a direct effect.
  • the initiators used in the research of non-heading cabbage seeds are mostly potassium nitrate, sodium chloride, calcium chloride and polyethylene glycol 6000.
  • Ascorbic acid is an important substance in the plant's non-enzymatic defense system against membrane lipid peroxidation. It has a strong reducing ability and can protect against membrane lipid peroxidation, scavenging free radicals, and maintaining the integrity of cell membranes.
  • exogenous ascorbic acid priming can significantly improve the germination of stressed rapeseed and soybean seeds, and can significantly improve the germination of old maca seeds. Its effect on the treatment of non-heading Chinese cabbage seeds has not been reported yet.
  • the purpose of the present invention is to provide a non-heading Chinese cabbage seed initiator and an application method thereof, so as to solve the problems raised in the background art.
  • An initiator for non-heading Chinese cabbage seeds includes ascorbic acid, calcium chloride, gibberellin, polyethylene glycol, buffer, and bactericide.
  • the concentration of the ascorbic acid is 20-200 mg/L.
  • the concentration of the ascorbic acid is 50 mg/L.
  • the concentration of the ascorbic acid is 80 mg/L.
  • the buffer is one or more of glucose and fumaric acid.
  • the bactericide is tetramethylthiuram disulfide.
  • the non-heading Chinese cabbage seed initiator and 500 non-heading Chinese cabbage seeds of various concentrations are placed in a triangular flask, and the initiation treatment is carried out under dark conditions at 20°C for 24 hours.
  • the non-heading cabbage seed initiator of the present invention controls the water absorption of seed cells through the initiation effect of ascorbic acid, which is beneficial to improve the germination ability of seeds and promote the germination of non-heading cabbage seeds.
  • the non-heading cabbage seed initiator of the present invention has the functions of preventing membrane lipid peroxidation, scavenging free radicals, and maintaining the integrity of cell membranes, which is more conducive to seedling growth.
  • the invention is easy to operate.
  • the non-heading cabbage seed initiator of the present invention has the advantages of natural environmental protection, easy degradation, non-toxic and harmless.
  • An initiator for non-heading Chinese cabbage seeds includes ascorbic acid, calcium chloride, gibberellin, polyethylene glycol, buffer, and bactericide.
  • the concentration of ascorbic acid is 20 mg/L
  • the buffer is glucose
  • the bactericide is tetramethylthiuram disulfide.
  • the application method of the non-heading cabbage seed initiator is to select plump, neat and consistent seeds, place them in a 150ml Erlenmeyer flask, put 500 seeds in each Erlenmeyer flask, and initiate treatment at 20°C under dark conditions for 24 hours.
  • An initiator for non-heading Chinese cabbage seeds includes ascorbic acid, calcium chloride, gibberellin, polyethylene glycol, buffer, and bactericide.
  • the concentration of the ascorbic acid is 50 mg/L
  • the buffer is fumaric acid
  • the bactericide is tetramethylthiuram disulfide.
  • the application method of the non-heading cabbage seed initiator is to select plump, neat and consistent seeds, place them in a 150ml Erlenmeyer flask, put 500 seeds in each Erlenmeyer flask, and initiate treatment at 20°C under dark conditions for 24 hours.
  • An initiator for non-heading Chinese cabbage seeds includes ascorbic acid, calcium chloride, gibberellin, polyethylene glycol, buffer, and bactericide.
  • the concentration of the ascorbic acid is 80 mg/L
  • the buffer is glucose
  • the bactericide is tetramethylthiuram disulfide.
  • the application method of the non-heading cabbage seed initiator is to select plump, neat and consistent seeds, place them in a 150ml Erlenmeyer flask, put 500 seeds in each Erlenmeyer flask, and initiate treatment at 20°C under dark conditions for 24 hours.
  • An initiator for non-heading Chinese cabbage seeds includes ascorbic acid, calcium chloride, gibberellin, polyethylene glycol, buffer, and bactericide.
  • the concentration of the ascorbic acid is 100 mg/L
  • the buffer is fumaric acid
  • the bactericide is tetramethylthiuram disulfide.
  • the application method of the non-heading cabbage seed initiator is to select plump, neat and consistent seeds, place them in a 150ml Erlenmeyer flask, put 500 seeds in each Erlenmeyer flask, and initiate treatment at 20°C under dark conditions for 24 hours.
  • An initiator for non-heading Chinese cabbage seeds includes ascorbic acid, calcium chloride, gibberellin, polyethylene glycol, buffer, and bactericide.
  • the concentration of the ascorbic acid is 150 mg/L
  • the buffer is a combination of glucose and fumaric acid
  • the ratio of glucose to fumaric acid is 1:1
  • the bactericide is tetramethylthiuram disulfide.
  • the application method of the non-heading cabbage seed initiator is to select plump, neat and consistent seeds, place them in a 150ml Erlenmeyer flask, put 500 seeds in each Erlenmeyer flask, and initiate treatment at 20°C under dark conditions for 24 hours.
  • An initiator for non-heading Chinese cabbage seeds includes ascorbic acid, calcium chloride, gibberellin, polyethylene glycol, buffer, and bactericide.
  • the concentration of the ascorbic acid is 200 mg/L
  • the buffer is glucose
  • the bactericide is tetramethylthiuram disulfide.
  • the application method of the non-heading cabbage seed initiator is to select plump, neat and consistent seeds, place them in a 150ml Erlenmeyer flask, put 500 seeds in each Erlenmeyer flask, and initiate treatment at 20°C under dark conditions for 24 hours.
  • the non-heading Chinese cabbage seed initiator of this comparative example includes: distilled water, calcium chloride, gibberellin, polyethylene glycol, buffer, and fungicide.
  • the buffering agent is glucose
  • the bactericide is tetramethylthiuram disulfide.
  • the application method of the non-heading cabbage seed initiator is to select plump, neat and consistent seeds, place them in a 150ml Erlenmeyer flask, put 500 seeds in each Erlenmeyer flask, and initiate treatment at 20°C under dark conditions for 24 hours.
  • test materials used in this experiment are the seeds of non-heading Chinese cabbage variety ‘Qinglan’ (harvested in 2013 and 2017) and ‘shulu’ (harvested in 2013 and 2017).
  • the seeds were washed with deionized water for 2 minutes, dried at 25°C, and then placed in a 9cm petri dish with two layers of filter paper and filled with water to reach saturation In the middle, the distance between seeds is placed 1-2 times the size of the seeds. Put 100 seeds in each petri dish, repeat 3 times with different varieties, different years and different concentrations. Place the germination test in an incubator at 22°C, 16h/17°C, and 8h temperature cycle under dark conditions.
  • the four germination indexes of seed germination vigor, germination rate, germination index and vigor index were measured.
  • the calculation method of each indicator is as follows:
  • Seed germination vigor (%) the number of germinated seeds at the initial stage (the 5th day)/the number of tested seeds*100%;
  • Germination index (GI) ⁇ Gt/Dt
  • V Vigor Index
  • Gt is the number of germinated seeds on different germination days (counting 7 days)
  • Dt is the corresponding germination days
  • S is the total length of the radicle and hypocotyl at the corresponding germination days.
  • Example 2 can significantly improve the germination potential, germination index and vitality index of seeds.
  • the germination potential, germination index and vitality index of the'green basket' seeds in 2013 increased by 4.16%, 3.72, 127.56, respectively, 2017
  • the seeds of the young'green basket' increased by 9.17%, 9.36 and 20.35 respectively.
  • the example treatment did not have a significant effect on its germination potential, but it achieved a certain range improvement compared with the comparative example.
  • Table 3 show that, except for the decrease in the vigor index of the seeds of Examples 4 and 5, the other examples all have different degrees of improving the germination vigor, germination rate, germination index and vigor index of the'Shulu' seeds.
  • the germination rates of Examples 2-5 all reached 100%.
  • the germination vigor and germination rate of Examples 2 and 3 were significantly improved, and the effect of Example 3 to significantly enhance the germination characteristics was the best.
  • the germination vigor, germination rate, germination index and vitality index of seeds were increased by 4.44 respectively. %, 0.83%, 3.73, 112.01.
  • Example 2 has the best effect on significantly improving the germination vigor, germination rate, germination index and vigor index of seeds, which are respectively increased by 8.61%, 5.83%, 5.06, 22.37 compared to the control.
  • the present invention provides a non-heading cabbage seed initiator, which improves the germination potential, germination rate, germination index and vigor index of non-heading cabbage seeds, and significantly enhances the seeds vitality.
  • the two optimal concentrations of ascorbic acid in the non-heading cabbage seed initiator are 50 and 80 mg/L respectively, which can significantly improve the germination ability of seeds.

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Abstract

本发明涉及种子引发技术领域，具体是一种不结球白菜种子引发剂，包括：抗坏血酸、氯化钙、赤霉素、聚乙二醇、缓冲剂、杀菌剂，所述抗坏血酸的浓度为20-200mg/L，所述不结球白菜种子引发剂中的抗坏血酸的两个最佳浓度分别为50和80mg/L，所述缓冲剂为葡萄糖、延胡索酸中的一种或多种，所述杀菌剂为四甲基秋兰姆二硫化物。本发明还公开了所述不结球白菜种子引发剂的应用方法。本发明不结球白菜种子引发剂通过抗坏血酸渗透引发作用，控制种子细胞吸水，有利于提高种子发芽力，促进不结球白菜种子萌发，具有防御膜脂过氧化及清除自由基、维持细胞膜的完整性的功能，更有利于幼苗生长。

Description

一种不结球白菜种子引发剂及其应用方法 技术领域
本发明涉及种子引发技术领域，具体是一种不结球白菜种子引发剂及其应用方法。
背景技术
种子引发(Seed priming)是在控制条件下使种子缓慢吸水，从而为萌发提前进行生理准备的一种播前种子处理技术，是提高种子活力的一种有效途径。种子引发技术已在蔬菜作物、农作物、经济作物、观赏植物、林木等多种植物种类上进行了成功应用，包括如茄子、辣椒、番茄、黄瓜、香瓜、甜瓜、西瓜、结球甘蓝、胡萝卜、芹菜、菠菜、大葱、洋葱等各类蔬菜；大麦、小麦、玉米、水稻和高粱等多种粮食作物；以及雪叶莲、马鞭草属、矮牵牛属和三色堇等多种观赏植物。通过引发处理获得了良好的发芽效果，提高了幼苗的整齐率和抗性，为其后期生长发育奠定良好基础。
不结球白菜(Brassica campestris ssp.chinensis Makino)属十字花科芸薹属芸薹种不结球白菜亚种，俗称青菜、小白菜、油菜等，是原产于我国的重要蔬菜作物。不结球白菜质地鲜嫩，营养丰富，深受人们喜爱，在我国蔬菜消费中占有重要地位。不结球白菜多采用直播方式栽培，种子颗粒小，存在播种量大、出苗率较低、幼苗素质差等实际生产问题，因此，提高种子活力及幼苗素质对不结球白菜的产量和质量都有直接影响作用。研究不结球白菜种子引发所用引发剂多采用硝酸钾、氯化钠、氯化钙及聚乙二醇6000等。
抗坏血酸(L-Ascorbic acid)是植物对膜脂过氧化非酶促防御系统中的一种重要物质，有较强的还原能力，具有防御膜脂过氧化及清除自由基、维持细胞膜的完整性的功能。已有研究表明，外源抗坏血酸引发可显著改善受胁迫的油菜、大豆种子的发芽状况，能显著提高玛咖陈种子的发芽力。其对于不结球白菜种子的处理效果尚未见报道。
发明内容
本发明的目的在于提供一种不结球白菜种子引发剂及其应用方法，以解决上述背景技术中提出的问题。
为实现上述目的，本发明提供如下技术方案：
一种不结球白菜种子引发剂，包括：抗坏血酸、氯化钙、赤霉素、聚乙二醇、缓冲剂、杀菌剂。
作为本发明进一步的方案：所述抗坏血酸的浓度为20-200mg/L。
作为本发明再进一步的方案：所述抗坏血酸的浓度为50mg/L。
作为本发明再进一步的方案：所述抗坏血酸的浓度为80mg/L。
作为本发明再进一步的方案：所述缓冲剂为葡萄糖、延胡索酸中的一种或多种。
作为本发明再进一步的方案：所述杀菌剂为四甲基秋兰姆二硫化物。
所述不结球白菜种子引发剂的应用方法，将各浓度的不结球白菜种子引发剂及500粒不结球白菜种子置于三角瓶中，在20℃黑暗条件下引发处理24h。
与现有技术相比，本发明的有益效果是：
1.本发明不结球白菜种子引发剂通过抗坏血酸渗透引发作用，控制种子细胞吸水，有利于提高种子发芽力，促进不结球白菜种子萌发。
2.本发明不结球白菜种子引发剂具有防御膜脂过氧化及清除自由基、维持细胞膜的完整性的功能，更有利于幼苗生长。
3.本发明易于操作。
4.本发明不结球白菜种子引发剂具有天然环保、易降解、无毒无害的优点。
具体实施方式
下面结合具体实施方式对本专利的技术方案作进一步详细地说明。
实施例1
一种不结球白菜种子引发剂，包括：抗坏血酸、氯化钙、赤霉素、聚乙二醇、缓冲剂、杀菌剂。
其中，所述抗坏血酸的浓度为20mg/L，所述缓冲剂为葡萄糖，所述杀菌剂为四甲基秋兰姆二硫化物。
所述不结球白菜种子引发剂的应用方法，挑选饱满、整齐一致的种子，置于150ml的三角瓶中，每个三角瓶中放入500粒种子，在20℃黑暗条件下引发处理24h。
实施例2
一种不结球白菜种子引发剂，包括：抗坏血酸、氯化钙、赤霉素、聚乙二醇、缓冲剂、杀菌剂。
其中，所述抗坏血酸的浓度为50mg/L，所述缓冲剂为延胡索酸，所述杀菌剂为四甲基秋兰姆二硫化物。
所述不结球白菜种子引发剂的应用方法，挑选饱满、整齐一致的种子，置于150ml的三角瓶中，每个三角瓶中放入500粒种子，在20℃黑暗条件下引发处理24h。
实施例3
一种不结球白菜种子引发剂，包括：抗坏血酸、氯化钙、赤霉素、聚乙二醇、缓冲剂、杀菌剂。
其中，所述抗坏血酸的浓度为80mg/L，所述缓冲剂为葡萄糖，所述杀菌剂为四甲基秋兰姆二硫化物。
所述不结球白菜种子引发剂的应用方法，挑选饱满、整齐一致的种子，置于150ml的三角瓶中，每个三角瓶中放入500粒种子，在20℃黑暗条件下引发处理24h。
实施例4
一种不结球白菜种子引发剂，包括：抗坏血酸、氯化钙、赤霉素、聚乙二醇、缓冲剂、杀菌剂。
其中，所述抗坏血酸的浓度为100mg/L，所述缓冲剂为延胡索酸，所述杀菌剂为四甲基秋兰姆二硫化物。
所述不结球白菜种子引发剂的应用方法，挑选饱满、整齐一致的种子，置于150ml的三角瓶中，每个三角瓶中放入500粒种子，在20℃黑暗条件下引发处理24h。
实施例5
一种不结球白菜种子引发剂，包括：抗坏血酸、氯化钙、赤霉素、聚乙二醇、缓冲剂、杀菌剂。
其中，所述抗坏血酸的浓度为150mg/L，所述缓冲剂为葡萄糖和延胡索酸的组合物， 且葡萄糖和延胡索酸的比例为1:1，所述杀菌剂为四甲基秋兰姆二硫化物。
所述不结球白菜种子引发剂的应用方法，挑选饱满、整齐一致的种子，置于150ml的三角瓶中，每个三角瓶中放入500粒种子，在20℃黑暗条件下引发处理24h。
实施例6
一种不结球白菜种子引发剂，包括：抗坏血酸、氯化钙、赤霉素、聚乙二醇、缓冲剂、杀菌剂。
其中，所述抗坏血酸的浓度为200mg/L，所述缓冲剂为葡萄糖，所述杀菌剂为四甲基秋兰姆二硫化物。
所述不结球白菜种子引发剂的应用方法，挑选饱满、整齐一致的种子，置于150ml的三角瓶中，每个三角瓶中放入500粒种子，在20℃黑暗条件下引发处理24h。
对比例
本对比例的不结球白菜种子引发剂包括：蒸馏水、氯化钙、赤霉素、聚乙二醇、缓冲剂、杀菌剂。
其中，所述缓冲剂为葡萄糖，所述杀菌剂为四甲基秋兰姆二硫化物。
所述不结球白菜种子引发剂的应用方法，挑选饱满、整齐一致的种子，置于150ml的三角瓶中，每个三角瓶中放入500粒种子，在20℃黑暗条件下引发处理24h。
1、种子发芽试验：
本试验中使用的供试材料为不结球白菜品种‘青篮’(2013年采收及2017年采收)、‘暑绿’(2013年采收及2017年采收)种子。
按照实施例1-6及对比例的方法处理后，将种子用去离子水洗2min，25℃条件下将种子晾干，然后将种子置于带有两层滤纸，注入清水达到饱和的9cm培养皿中，种子间距离按种子大小的1-2倍摆放。每个培养皿放入100粒种子，不同品种、不同年份、不同浓度3次重复。放在22℃，16h/17℃，8h温度周期的黑暗条件下的培养箱内进行发芽试验。
2、发芽指标测定
测定种子的发芽势、发芽率、发芽指数和活力指数4项发芽指标。各指标计算方法如 下:
种子发芽势(％)＝初期(第5天)发芽种子数/供试种子数*100％；
种子发芽率(％)＝终期(第7天)发芽种子数/供试种子数*100％；
发芽指数(GI)＝∑Gt/Dt；
活力指数(VI)＝∑Gt/Dt*S；
其中Gt为不同发芽天(计数7d)的发芽种子数，Dt为相应发芽天数，S为相应发芽天数时的胚根及胚轴的总长度。
3、结果及分析
将按照实施例1-6及对比例的方法处理后的不结球白菜‘青篮’种子，按照1中提及的方法进行萌发试验，并将实验结果记录如表1、表2所示。
表1不同处理方法对不结球白菜‘青篮’种子(2013年采收)发芽的影响
Figure PCTCN2019125941-appb-000001
注：同列数据中不同字母表示处理间在0.05水平有显著性差异；下同。
表2不同处理方法对不结球白菜‘青篮’种子(2017年采收)发芽的影响
Figure PCTCN2019125941-appb-000002
Figure PCTCN2019125941-appb-000003
由表1、表2可知，在实施例处理下，‘青篮’种子的发芽势、发芽率、发芽指数和活力指数的变化大致呈先增高后降低的趋势。实施例2可显著提高种子的发芽势、发芽指数和活力指数，与对比例相比，2013年‘青篮’种子的发芽势、发芽指数和活力指数分别提高了4.16％、3.72、127.56，2017年‘青篮’种子则分别提高了9.17％、9.36、20.35。实施例处理对其发芽势未产生显著影响，但相较对比例获得了一定范围的提高。
将按照实施例1-6及对比例的方法处理后的不结球白菜‘暑绿’种子，按照1中提及的方法进行萌发试验，并将实验结果记录如表3、表4所示。
表3不同处理方法对不结球白菜‘暑绿’种子(2013年采收)发芽的影响
Figure PCTCN2019125941-appb-000004
表4不同处理方法对不结球白菜‘暑绿’种子(2017年采收)发芽的影响
Figure PCTCN2019125941-appb-000005
Figure PCTCN2019125941-appb-000006
表3结果显示，除实施例4、5种子的活力指数有所降低以外，其他各实施例均对‘暑绿’种子的发芽势、发芽率、发芽指数和活力指数具有不同程度的提高作用。实施例2-5的发芽率均达到了100％。相比对比例，实施例2、3的发芽势和发芽率均获得显著提高，且实施例3显著增强发芽特性的效果最佳，种子的发芽势、发芽率、发芽指数、活力指数分别提高4.44％、0.83％、3.73、112.01。
由表4可知，各实施例的发芽势、发芽率、发芽指数及实施例1、2的活力指数均获得了一定的提高。实施例2显著提高种子的发芽势、发芽率、发芽指数和活力指数的效果最佳，相比对比例分别提高8.61％、5.83％、5.06、22.37。
综上所述，本发明提供了一种不结球白菜种子引发剂，该不结球白菜种子引发剂提高了不结球白菜种子的发芽势、发芽率、发芽指数和活力指数，显著增强种子活力。该不结球白菜种子引发剂中的抗坏血酸的两个最佳浓度分别为50和80mg/L，可以显著提高种子的发芽力。
上面对本专利的较佳实施方式作了详细说明，但是本专利并不限于上述实施方式，在本领域的普通技术人员所具备的知识范围内，还可以在不脱离本专利宗旨的前提下做出各种变化。

Claims (7)

  1. 一种不结球白菜种子引发剂，其特征在于，包括：抗坏血酸、氯化钙、赤霉素、聚乙二醇、缓冲剂、杀菌剂。
  2. 根据权利要求1所述的不结球白菜种子引发剂，其特征在于，所述抗坏血酸的浓度为20-200mg/L。
  3. 根据权利要求2所述的不结球白菜种子引发剂，其特征在于，所述抗坏血酸的浓度为50mg/L。
  4. 根据权利要求2所述的不结球白菜种子引发剂，其特征在于，所述抗坏血酸的浓度为80mg/L。
  5. 根据权利要求1所述的不结球白菜种子引发剂，其特征在于，所述缓冲剂为葡萄糖、延胡索酸中的一种或多种。
  6. 根据权利要求1所述的不结球白菜种子引发剂，其特征在于，所述杀菌剂为四甲基秋兰姆二硫化物。
  7. 一种如权利要求1-6任一所述的不结球白菜种子引发剂的应用方法，其特征在于，将各浓度的不结球白菜种子引发剂及500粒不结球白菜种子置于三角瓶中，在20℃黑暗条件下引发处理24h。
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