WO2020173511A3 - 一种佐剂生物活性的检测方法 - Google Patents

一种佐剂生物活性的检测方法 Download PDF

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Publication number
WO2020173511A3
WO2020173511A3 PCT/CN2020/087534 CN2020087534W WO2020173511A3 WO 2020173511 A3 WO2020173511 A3 WO 2020173511A3 CN 2020087534 W CN2020087534 W CN 2020087534W WO 2020173511 A3 WO2020173511 A3 WO 2020173511A3
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WO
WIPO (PCT)
Prior art keywords
adjuvant
biological activity
measuring
present
quality
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PCT/CN2020/087534
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English (en)
French (fr)
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WO2020173511A2 (zh
Inventor
赵干
何忠淮
俞庆龄
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艾棣维欣(苏州)生物制药有限公司
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Publication of WO2020173511A2 publication Critical patent/WO2020173511A2/zh
Publication of WO2020173511A3 publication Critical patent/WO2020173511A3/zh

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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6863—Cytokines, i.e. immune system proteins modifying a biological response such as cell growth proliferation or differentiation, e.g. TNF, CNF, GM-CSF, lymphotoxin, MIF or their receptors

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Engineering & Computer Science (AREA)
  • Cell Biology (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)

Abstract

一种佐剂生物活性的检测方法，包括以下步骤：将佐剂或佐剂/共刺激物混合物作用于细胞模型；检测细胞模型分泌的蛋白因子量，用以表征佐剂的生物学活性。该检测方法及相应的检测试剂能精确鉴别不同佐剂的生物学活性，从而简化了佐剂的评价手段，该检测方法易操作、实用性强、应用面广，可应用于含佐剂疫苗质量的快速评价中。
PCT/CN2020/087534 2019-02-28 2020-04-28 一种佐剂生物活性的检测方法 WO2020173511A2 (zh)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
CN201910153732.7A CN109752560A (zh) 2019-02-28 2019-02-28 一种佐剂生物活性的检测方法
CN201910153732.7 2019-02-28
CN202010115594.6 2020-02-25
CN202010115594.6A CN111122878B (zh) 2019-02-28 2020-02-25 一种佐剂生物活性的检测方法

Publications (2)

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WO2020173511A2 WO2020173511A2 (zh) 2020-09-03
WO2020173511A3 true WO2020173511A3 (zh) 2020-10-22

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CN (2) CN109752560A (zh)
WO (1) WO2020173511A2 (zh)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109752560A (zh) * 2019-02-28 2019-05-14 艾棣维欣(苏州)生物制药有限公司 一种佐剂生物活性的检测方法
CN110456042B (zh) * 2019-08-09 2022-07-05 臻悦生物科技江苏有限公司 血清学生物标志物在制备试剂盒和/或芯片中的应用、试剂盒或芯片及其制备方法

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20120258125A1 (en) * 2011-04-06 2012-10-11 Lancell, L.L.C. Method to identify a novel class of immunologic adjuvants
CN103412085A (zh) * 2013-08-27 2013-11-27 成都生物制品研究所有限责任公司 一种氨水制备的氢氧化铝佐剂吸附能力的检测方法
CN106456745A (zh) * 2014-02-11 2017-02-22 北京艾棣维欣生物技术有限公司 具有白细胞介素‑17作为佐剂的疫苗
JP2018068177A (ja) * 2016-10-27 2018-05-10 日清食品ホールディングス株式会社 アジュバント作用の評価法
CN109752560A (zh) * 2019-02-28 2019-05-14 艾棣维欣(苏州)生物制药有限公司 一种佐剂生物活性的检测方法

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Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20120258125A1 (en) * 2011-04-06 2012-10-11 Lancell, L.L.C. Method to identify a novel class of immunologic adjuvants
CN103412085A (zh) * 2013-08-27 2013-11-27 成都生物制品研究所有限责任公司 一种氨水制备的氢氧化铝佐剂吸附能力的检测方法
CN106456745A (zh) * 2014-02-11 2017-02-22 北京艾棣维欣生物技术有限公司 具有白细胞介素‑17作为佐剂的疫苗
JP2018068177A (ja) * 2016-10-27 2018-05-10 日清食品ホールディングス株式会社 アジュバント作用の評価法
CN109752560A (zh) * 2019-02-28 2019-05-14 艾棣维欣(苏州)生物制药有限公司 一种佐剂生物活性的检测方法

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* Cited by examiner, † Cited by third party
Title
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WANG, HUI: "Immunoregulation of the Polysaccharides from the Roots of Actinidia Eriantha on RAW264.7 Cell and Its Mechanisms", WANFANG DATA, 22 May 2014 (2014-05-22), DOI: 20200626102054X *
WANG, HUI: "Immunoregulation of the Polysaccharides from the Roots of Actinidia Eriantha on RAW264.7 Cell and Its Mechanisms", WANFANG DATA, 22 May 2014 (2014-05-22), DOI: 20200626102102Y *

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WO2020173511A2 (zh) 2020-09-03
CN111122878A (zh) 2020-05-08
CN109752560A (zh) 2019-05-14
CN111122878B (zh) 2020-11-20

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