WO2020171652A2 - Système de détection de particules sous-marines en temps réel au moyen de bulles - Google Patents

Système de détection de particules sous-marines en temps réel au moyen de bulles Download PDF

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Publication number
WO2020171652A2
WO2020171652A2 PCT/KR2020/002548 KR2020002548W WO2020171652A2 WO 2020171652 A2 WO2020171652 A2 WO 2020171652A2 KR 2020002548 W KR2020002548 W KR 2020002548W WO 2020171652 A2 WO2020171652 A2 WO 2020171652A2
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bubble
real
detection system
time
image
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PCT/KR2020/002548
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English (en)
Korean (ko)
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WO2020171652A3 (fr
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정영수
김지윤
이승현
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숙명여자대학교산학협력단
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Publication of WO2020171652A2 publication Critical patent/WO2020171652A2/fr
Publication of WO2020171652A3 publication Critical patent/WO2020171652A3/fr

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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F23/00—Mixing according to the phases to be mixed, e.g. dispersing or emulsifying
    • B01F23/20—Mixing gases with liquids
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F23/00—Mixing according to the phases to be mixed, e.g. dispersing or emulsifying
    • B01F23/20—Mixing gases with liquids
    • B01F23/23—Mixing gases with liquids by introducing gases into liquid media, e.g. for producing aerated liquids
    • B01F23/231—Mixing gases with liquids by introducing gases into liquid media, e.g. for producing aerated liquids by bubbling
    • B01F23/23105—Arrangement or manipulation of the gas bubbling devices
    • B01F23/2312—Diffusers
    • B01F23/23121—Diffusers having injection means, e.g. nozzles with circumferential outlet
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/18—Water
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
    • G01N2021/1765—Method using an image detector and processing of image signal
    • G01N2021/177—Detector of the video camera type
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2201/00—Features of devices classified in G01N21/00
    • G01N2201/06—Illumination; Optics
    • G01N2201/068—Optics, miscellaneous

Definitions

  • the present invention relates to a real-time underwater particle detection system using bubbles.
  • biochemical oxygen demand (BOD) or chemical oxygen demand (COD) has disadvantages such as long analysis time and low reproducibility.
  • BOD biochemical oxygen demand
  • COD chemical oxygen demand
  • the current water quality level cannot be reflected in real time because the water collected at the site is moved to another place when measuring water quality.
  • An object of the present invention is to provide a real-time underwater particle detection system using a bubble capable of qualitative and quantitative analysis since particles inside a bubble can be directly visualized.
  • the present invention is to provide a real-time underwater particle detection system capable of realizing accurate reproducibility by measuring water quality in real time without being subject to temporal and spatial constraints.
  • the present invention provides a bubble generating device for generating a bubble, a light emitting unit for irradiating light to the generated bubble, and collecting the light scattered from the bubble to obtain an image of the bubble. It provides a real-time underwater particle detection system including an image sensor unit.
  • the bubble generating device may generate a bubble having a water film on a liquid surface or a water film in a frame.
  • a bubble bursting device for bursting the bubble, a droplet collecting device for collecting droplets generated when the bubble bursts, and acquiring an image or an electromagnetic signal of the droplets collected by the droplet collecting device It may further include a sensor unit including a droplet sensor.
  • the bubble generating device includes a tube in which the bubble is generated at one end, and an air injection unit that supplies air to the tube to generate the bubble at one end of the tube. can do.
  • a solution containing water and a surfactant may be accommodated inside the tube.
  • a control unit may further include a control unit that detects at least one of the presence or absence of bacteria and concentration in the liquid using the image of the bubble obtained from the image sensor unit.
  • the sensor unit may further include a bubble burst control device configured to rupture the generated bubbles by operating the bubble bursting device according to a preset time or user input.
  • the droplet collecting device includes a sampling mechanism or a sampling plate on which the collected droplets are seated, and the sampling mechanism or the sampling plate may be detachable.
  • the droplet sensor includes a droplet image sensor that acquires a digital image or a fluorescence image of the droplet collected from the droplet collecting device, and a droplet electromagnetic signal sensor that acquires an electromagnetic signal applied to the droplet. can do.
  • the U-shaped tube has a first end in which the bubble is not formed and a second end in which the bubble is formed, but the heights of the first and second ends may be different.
  • the light emitting unit is a light source, a plurality of lenses spaced apart from the light source, and a pinhole disposed between any two lenses selected from among the plurality of lenses, and located on the optical path of the light source. It may include a pinhole plate with a pin hole.
  • the light source may be visible light.
  • the plurality of lenses include first to fourth lenses sequentially spaced apart from the light source, and the first and second lenses are plano convex lenses, but are disposed so that the convex surfaces face each other.
  • the pinhole plate may be positioned between the second and third lenses.
  • the third lens may be disposed to irradiate light parallel to the height of the bubble generated in the tube.
  • the image sensor unit includes: a first camera for photographing an image of a bubble generated in the tube, a fourth lens for condensing light scattered from the generated bubble, and a fourth lens passing through the fourth lens. It may include a second camera to acquire an image.
  • control unit measures the number of black spots and/or white spots extracted from the image of the bubble obtained by the image sensor unit, and exists in the bubble.
  • concentration of the particles may be calculated, but the concentration of the particles may be calculated using information about the time when the bubble was generated.
  • control unit stores spot data including a unique surface energy value for each type of the particle, and the black spot extracted from the image of the bubble obtained by the image sensor unit ) And/or the size, thickness, and period of the light pattern formed around each of the white spots may be applied to the spot data to calculate the particle type data.
  • the control unit measures the survival time of the bubble by using the image of the bubble acquired by the image sensor unit, and stores the time set by the user in a memory to determine the survival time of the bubble.
  • the bubble bursting device is operated to burst the bubble, or the bubble bursting device is operated according to a user's input to burst the bubble, and the thickness of the bubble film can be calculated based on the bursting rate of the bubble. have.
  • the present invention provides a bubble generator for generating a bubble, a transmitter for irradiating an electromagnetic signal to the generated bubble, a receiver for acquiring the electromagnetic signal transmitted through the bubble, and an electromagnetic signal transmitted through the receiver.
  • a real-time underwater particle detection system including a control unit for detecting at least one of the presence or absence and concentration of particles in the liquid.
  • the real-time underwater particle detection system using bubbles enables real-time quantitative analysis of particles through visualization by generating bubbles, and can analyze the type of particles in real time through the surface characteristics and simulation of the particles, and thus detection cost This is low, measurement time is short, and the system can be miniaturized and modularized.
  • the real-time underwater particle detection system using a bubble according to the present invention can predict the concentration of particles in water and the concentration of particles contained in an aerosol generated when the bubble bursts.
  • it can be used to determine the type of particle through statistical analysis or predicting characteristics using image analysis or computer simulation based on the surface characteristics of particles.
  • the present invention can perform the same analysis by creating a thin water film instead of a bubble shape using a ring-like frame, and in this case, a tube and an air injection unit are not required, so that the overall system can be miniaturized.
  • 1 is a diagram showing a step-by-step method of determining the amount and type of bacteria in water in the related art.
  • FIG. 2 is a diagram schematically showing the configuration of a real-time underwater particle detection system according to an embodiment of the present invention.
  • FIG. 3 is a block diagram of a real-time underwater particle detection system according to an embodiment of the present invention.
  • FIG. 4 is a view showing a tube according to an embodiment of the present invention.
  • FIG. 5 is a view showing an air injection unit according to an embodiment of the present invention.
  • FIG. 6 is a view showing a light emitting unit according to an embodiment of the present invention.
  • FIG. 7 is a view showing an image sensor unit according to an embodiment of the present invention.
  • FIG. 8 is a view for explaining the principle of the real-time underwater particle detection system according to an embodiment of the present invention detects bacteria existing in the water.
  • FIG. 9 is a diagram showing in detail a sensor unit collecting droplets generated when a bubble bursts according to an embodiment of the present invention.
  • FIG. 10 is a diagram schematically showing a method of popping a bubble according to an embodiment of the present invention.
  • FIG. 11 is a diagram showing a curve formed by a surface of a bubble generated according to an embodiment of the present invention and a surface of a particle trapped therein.
  • FIG. 12 is a diagram showing an image of bacteria in a bubble.
  • FIG. 13 is a diagram illustrating an image of bacteria in a water film by forming a thin water film containing fine particles by a bubble generating device using a ring-structured frame according to an embodiment of the present invention.
  • FIG. 2 is a diagram schematically showing the configuration of a real-time underwater particle detection system according to an embodiment of the present invention
  • FIG. 3 is a configuration diagram of a real-time underwater particle detection system according to an embodiment of the present invention.
  • the real-time underwater particle detection system 100 includes a bubble generating device that generates bubbles, and an outgoing light that irradiates light to the generated bubbles.
  • a bubble generating device that generates bubbles
  • an outgoing light that irradiates light to the generated bubbles.
  • FIGS. 2 and 3 since the components shown in FIGS. 2 and 3 are not essential, a real-time underwater particle detection system having more or fewer components than that can be implemented.
  • particles refer to fine particles, and are not a term that distinguishes between living and inanimate objects, and means small particles ranging in size from several hundred nanometers to several hundred micrometers.
  • it may be expressed as a contaminant including bacteria, viruses, fungi, yeast, red blood cells, white blood cells, cells, algae, spores, microplastics, etc.
  • the bubble generating device is a device for creating a thin water film, for example, a device that generates a bubble with a thin water film on a liquid surface, or a thin water film in the frame using a polygonal or ring-shaped frame. .
  • a description will be made on the basis of a bubble, which is a representative example of a thin water film, but it is not intended to limit the scope of the present invention to a bubble. That is, when a thin liquid film is generated according to an embodiment of the present invention, it is important to know the thickness information of the water film at the time when the particles are detected, which is determined by the speed at which the water film is artificially burst, or Alternatively, it can be determined by analyzing the wavelength of light passing through the water film.
  • the bubble generating device can generate bubbles on the surface of the liquid by injecting gas into a container containing a liquid to generate bubbles.
  • the bubble generating device may include a container 10 for accommodating a liquid and an air injection unit 20 for injecting gas in the liquid.
  • the bubble generating device may generate a thin film of water in the frame by immersing the ring-shaped frame in the container 10 and then removing it, as shown in FIG. 13.
  • the description will be made based on bubbles for easy description of the present invention, but the scope of the present invention is not limited thereto.
  • the container 10 used by the bubble generating device to inject gas to generate bubbles on the liquid surface is not particularly limited in shape or size, but according to a preferred embodiment, shown in FIGS. 3 to 5 As such, it may be a tubular, but may be a U-shaped or J-shaped tube bent so that both ends of the tubular face the same direction.
  • the container 10 containing the liquid therein is provided with the first end so that the gas can be injected into the liquid in the same direction as the second end 12 in which the bubble is formed so that bubbles can be easily generated on the water surface at one end. 2 It is preferable that the first end 11 is formed in the same direction as the end 12, and more preferably the container 10 so that the height of the water surface where bubbles are generated can be easily adjusted using the pressure inside the tube. ) Is preferably a J-shaped.
  • the tube is a preferred embodiment of the container, and the terms tube and container are used interchangeably.
  • the diameter of one end of the container 10 is not particularly limited, but it is preferably formed to be thin so as to correspond to the diameter of the formable bubble.
  • the tube diameter is preferably 1.2 to 2.5 times, preferably 1.5 to 2.5 times the diameter of the generated bubble.
  • the curved portion of the bubble film formed on the water surface was about 2.35 times the average size of 6.38 mm.
  • FIG. 4 is a view showing a tube according to an embodiment of the present invention.
  • the U-shaped tube 13 according to a preferred embodiment of the container 10 has a first end 11 in which bubbles are not formed and a second end 12 in which bubbles are formed.
  • the U-shaped tube 13 is preferably J-shaped so that the first and second ends 11 and 12 have different heights based on the bent portions, as described above.
  • the tube 10 accommodates a liquid therein, but the liquid may be a solution containing water and a surfactant so as to easily form bubbles. At this time, by controlling the concentration of the surfactant contained in the solution, it is possible to control the life time of the bubble.
  • the type of surfactant is not particularly limited, but according to an embodiment, 1 selected from the group consisting of sodium dodecyl sulfate (SDS), dodecanol, and cetrimonium bromide (CTAB). It can be more than a species.
  • the concentration of the surfactant may be a critical micelle concentration (CMC) or higher.
  • FIG. 5 is a view showing an air injection unit according to an embodiment of the present invention.
  • the air injection unit 20 is for supplying outside air or gas in the gas container through a hose (or a nozzle), and according to an embodiment of the present invention, as shown in FIG. 5, air is supplied to supply gas.
  • the discharged hose may include an air pump 21 connected to one end of the U-shaped tube 13.
  • the air pump 21 is driven by a control command generated by the control unit 22 and supplies air to the U-shaped tube 13 filled with a solution in which water and surfactant are dissolved through the hose 25a.
  • bubbles can be generated at the second end 12 of the tube.
  • the control unit 22 continuously generates bubbles while bubbles are formed on the surface of the liquid, but to prevent collisions between bubbles. The formation time of the bubble can be controlled.
  • the size of the bubble formed at the second end 12 may be controlled according to the diameter and/or position of the hose (or nozzle).
  • the syringe 24 can inject the liquid contained therein into the tube 10 through the hose 25b.
  • the syringe ( 24) accommodates distilled water therein, and distilled water may be injected into the U-shaped tube 13.
  • a fluid supplied to the U-shaped tube 13 through a syringe 24 to a hose branched from a point on the hose 25b connected between the syringe 24 and the tube 10 It may include a buffer tank (buffer tank, 23) for accommodating the same type of fluid contained in the syringe (24) to keep the pressure constant.
  • the hose 25b connected to the buffer tank 23 and the syringe 24 is connected to the first end 11 in which no bubbles are generated in the U-shaped tube 13, and is connected to the other end of the U-shaped tube 13. It is possible to maintain a constant height of the bubble. That is, due to the evaporation generated when the bubble bursts, it can be kept constant without changing the height of the regenerated bubble when regenerating the bubble.
  • 3-way valves are provided to allow the fluid in the tube 10 to flow into the syringe 24 and/or It is possible to prevent backflow to the buffer tank 23.
  • the light emitting unit 30 is for irradiating light toward a bubble formed at one end of the tube 10, and may include a light source 31 that emits light.
  • FIG. 6 is a view showing a light emitting unit according to an embodiment of the present invention.
  • the light emitting unit 30 includes a light source 31, a plurality of lenses 32 to 34 spaced apart from the light source 31, and any two lenses selected from among the plurality of lenses. It is disposed between the (33, 34), it may include a pinhole plate 35 having a pin hole (pin hole) located on the light path emitted from the light source 31.
  • the plurality of lenses 32 to 34 may be three, but the number is not particularly limited, as long as the number of the lenses 32 to 34 is clearly acquired, and the type of each lens 32 to 34 is also Not limited.
  • the wavelength range of light emitted by the light source 31 may be visible light of 380 nm to 780 nm, and preferably the optimal wavelength range of light emitted by the light source 31 in order for the image sensor unit 40 to acquire a high-resolution image Is preferably 450nm to 640nm.
  • the type of the plurality of lenses 32 to 34 spaced apart from and arranged in front of the light source 31 is not particularly limited as long as it is for making the optical system described below.
  • the first and second lenses 32 and 33 are planar convex lenses to remove chromatic aberration, but the two convex lenses may be disposed so that the convex surfaces face each other, and the third lens 34 uses the light source 31. It may be a planar concave lens with a flat incident surface facing and a concave exit surface.
  • the light emitting unit 30 may further include a pinhole plate 35 disposed between the second and third lenses 33 and 34 to adjust the focus so that a clear image is formed on the camera, in which case the pinhole A pin hole formed through the plate 35 may be disposed to be positioned on an optical path passing through at least one lens.
  • the light that has passed through the first and second lenses 32 and 33 may pass through the pinhole 35 and then be inverted at the same time as a focal point, and the light that has passed through the pinhole 35 is a third lens ( After entering the plane of 34), the image sensor unit 40 is projected onto the subject bubble by being projected on the concave surface, and focusing through the fourth lens 42 located in front of the image sensor unit 40, the image sensor unit 40 is You can acquire the image of the bubble.
  • the third lens 34 is positioned to correspond to the height of the bubble generated at one end of the tube 10, so that the light that has passed through the third lens 34 can be irradiated at a height parallel to the bubble. It is desirable to do.
  • the real-time underwater particle detection system 100 may include an image sensor unit 40 for acquiring an image of the generated bubble.
  • FIG. 7 is a view showing an image sensor unit according to an embodiment of the present invention.
  • the image sensor unit 40 is a first camera that is spaced apart from the U-shaped tube 13 to obtain an image of the bubble generated in the tube 10. (41), a fourth lens (42) that condenses light scattered from the generated bubble, and a second camera (43) that transmits through the fourth lens (42) to obtain an image of the bubble, which is a subject. It may include.
  • the first camera 41 is not particularly limited, but may be a microscope camera, and the control unit 22 uses the image of the bubble 14 acquired through the first camera 41. The generation and disappearance of the bubble 14 can be checked and recognized. According to an embodiment, when the control unit 22 recognizes that the bubble 14 has burst and disappears, the air pump 21 may be driven and controlled to generate another bubble.
  • the second camera 43 is also not particularly limited, but may be a high speed camera, and the second camera 43 is a camera capable of photographing from the generation of bubbles to the process of exploding and extinguishing. Bacterial movement can be acquired. Likewise, the control unit 22 may check and recognize the generation and disappearance of the bubble 14 using the image of the bubble 14 acquired through the second camera 43.
  • Reference numeral 36 which is not described in FIG. 3, is a schematic diagram of a bacteria image.
  • FIG. 8 is a diagram for explaining a principle in which a real-time underwater particle detection system according to an embodiment of the present invention detects bacteria existing in water.
  • Light that is not refracted by bacteria is collected at one focal point through the first to fourth lenses 32 to 34 and 42, and the second camera 43 acquires a normal image of a bubble, but light refracted by bacteria Since silver cannot be collected in one focus, a black pattern is generated on the image acquired by the second camera 43, and bacteria existing in the water can be detected using the image of the bubble in real time.
  • control unit 60 may detect the presence or absence of bacteria or the concentration of bacteria by using the image obtained by the image sensor unit 40.
  • the control unit 60 may store data on the number of bacteria in bubbles according to the concentration of bacteria in water, and a black spot extracted from the image obtained by the image sensor unit 40 and / Or, by measuring the number of white spots, the concentration of bacteria present in the bubble can be calculated.
  • a spot is a light that is refracted by underwater bacteria captured by a bubble, and the concentration of bacteria can be calculated based on the number of spots.
  • the control unit 60 It is better to calculate the concentration of bacteria using this generated time information.
  • the time information is information that can predict the film thickness of the bubble (because the film thickness of the bubble depends on the survival time of the bubble), and using this, the control unit 60 can predict the actual size of the spot. .
  • the concentration of particles present on the bubble can be calculated using the actual size information and the number of spots measured in this way.
  • the size of the spot may be influenced by the curve formed on the surface 142 of the bubble by the captured bacteria 141 (see FIG. 11). Since the size of the particles that can be trapped in the bubble film depends on the film thickness (h) and contact angle ( ⁇ ) within a certain range (see FIG. 11), the control unit 60 uses the image sensor unit 40 to To calculate the concentration of bacteria by tracking the change in the curve formed by the bacteria 141 trapped on the surface of and measuring the number of spots when the film thickness (h) of the bubble is 0.5 ⁇ m to 100 ⁇ m. good.
  • the film thickness (h) of the bubble gradually decreases after the bubble is created, so the thickness (h) after a predetermined period of time (or a specific lifetime of the bubble) has elapsed. This is because the maximum number of spots can be observed when is the optimum thickness for capturing particles.
  • the film thickness h of the bubble has a thickness from 0.5 ⁇ m to 100 ⁇ m, the optimum particle average size observed in a film of this specific thickness is 5 times the film thickness.
  • control unit 60 may store spot data including a unique surface energy value for each type of underwater particle.
  • a light pattern may be formed around each of the black spots and/or white spots extracted from the image of the bubble obtained by the image sensor unit 40, and the size and thickness of the light pattern And a period (for example, an intensity period) to the spot data, it is possible to calculate the type of underwater particles.
  • a curve may be formed on the surface 142 of the bubble by trapped particles (or bacteria 141), and the curve formed at this time is the size of the trapped particles, the contact angle, and the thickness of the bubble film.
  • the contact angle ⁇ is according to the intrinsic surface energy value, and since it differs according to the type of underwater particles (or bacteria), the control unit 60 provides information on the curve or the light pattern formed around the spot.
  • the type of particle (or bacteria) may be calculated using information (the light pattern varies depending on the contact angle).
  • control unit 60 uses a bubble image, and as shown in FIG. 12, FIG. 12(a) is Bacillus, FIGS. 12(b) and (c) are E. coli, and FIG. 12(d) ) Can distinguish between Pseudomonas bacteria.
  • control unit 60 measures the survival time of the bubble 14 using the sensor unit 50 and/or the image sensor unit 40 that monitors the bubble, and stores the time set by the user in the memory.
  • the survival time of the bubble 14 coincides with the bubble burst control device 52 to which they are connected, the bubble 14 bursts, or the bubble burst control device 52 is operated according to the user's input to quickly bubble the bubble. You can burst (14) to generate droplets.
  • the control unit 60 After bursting the bubbles 14 as described above to generate droplets, the control unit 60 acquires digital images, fluorescence images, and electromagnetic signals of the droplets collected by the liquid crystal collecting device 53 to determine the size and number of droplets. , The content can be analyzed, and the analysis result is compared with the image analysis result of the bubble, thereby verifying the image analysis result of the bubble.
  • the droplet collecting apparatuses 53 and 54 are for collecting droplets scattered by bursting of the bubbles, and may include a sampling mechanism or a sampling plate for seating droplets in order to analyze the collected droplets.
  • the sampling device or the sampling plate included in the droplet collection devices 53 and 54 can be manually or automatically detached and mounted for replacement or cleaning.
  • FIG. 9 is a diagram showing in detail a sensor unit including a droplet collecting device for collecting droplets generated when a bubble bursts according to an embodiment of the present invention.
  • the sensor unit 50 includes a bubble bursting device 51 for bursting bubbles, and a droplet collecting device 53 for collecting droplets generated when the bubble bursts. And, it may include a droplet sensor (54, 55) for acquiring an image or an electromagnetic signal of the droplet from the droplet collecting device 53.
  • droplets generated from bubbles ruptured by the bubble bursting device 51 that bursts the bubbles 14 may be collected (or collected) by the droplet collecting device 53.
  • the droplet collecting device 53 may include a sampling mechanism or a sampling plate, and the sampling mechanism may be, for example, a conductive or non-conductive substrate including glass.
  • the control unit 60 may calculate the presence or absence of bacteria or concentration based on the image of the droplets collected by the droplet collecting device 53. That is, since the droplets collected in the droplet collecting device 53 contain bacteria (or contaminants) trapped in the bubble, the control unit 60 displays an image of the droplets collected in the droplet collecting device 53. Use to calculate the presence or absence of bacteria (or contaminants), or to calculate the concentration using the number.
  • the image acquired by the droplet image sensor 54 may be a general digital camera image, a fluorescence image, or a high magnification image such as a microscope.
  • control unit 60 may obtain an electromagnetic signal applied to the droplets collected by the droplet collecting device 53 by using the droplet electromagnetic field signal sensor 55, and the presence or absence of bacteria using the electromagnetic signal Concentration can be calculated.
  • the control unit 60 is based on the electromagnetic wave material constant according to the species of the bacteria. Presence or concentration can be calculated. Since the terahertz wave transmits well through various materials and is harmless to the human body and food unlike X-rays, the electromagnetic signal is preferably a terahertz wave.
  • the control unit 60 measures the size of the droplets, the number of droplets, and microorganisms or contaminants contained in the droplets by using an image or an electromagnetic signal of the droplets collected in the droplet collecting device 53.
  • the concentration and type of can be analyzed.
  • the light emitting unit 30 emitting light may include a transmitter (not shown) that generates an electromagnetic signal (eg, a terahertz wave) and irradiates it toward the bubble,
  • the sensor unit 50 specifically the droplet electromagnetic field signal sensor 55, obtains the electromagnetic signal transmitted through the bubble, so that the control unit 60 directly controls the presence, number, or concentration of particles contained in the bubble.
  • the type of particle can be determined.
  • FIG. 10 is a diagram schematically showing a method of popping a bubble according to an embodiment of the present invention.
  • the sensor unit 50 may include a bubble bursting device 51 that bursts bubbles, and the bubble bursting device 51 is driven by a control command from the bubble bursting control device 52.
  • the bubble bursting control device 52 may rupture the bubble by driving and controlling the bubble bursting device 51 by a control command of the control unit 60.
  • control unit 60 and/or the sensor unit 50 is an image obtained by the first camera 41 for the interval between the generation time of the bubble and the extinguishing time due to rupture, that is, a life time. It can be calculated using, and when the survival time reaches the survival time of the preset bubble, a control command is transmitted to the bubble bursting device 51 that moves the thin needle, and the needle contacts the bubble to burst and extinguish the bubble. I can.
  • control unit 60 forcibly ruptures the bubble according to the survival time of the bubble, and measures the rupture rate of the bubble, that is, the rate at which the hole increases, so that the thickness of the bubble film is inversely proportional to the square of the rupture rate of the bubble,
  • the thickness of the bubble film can be calculated using the bursting rate of the bubble.
  • the control unit 60 may calculate the size of the particles trapped in the bubble, as described above, using the calculated thickness of the bubble film.
  • FIG. 13 is a diagram illustrating an image of bacteria in the water film by forming a thin water film containing fine particles in the bubble generating apparatus according to an embodiment of the present invention using a ring-structured frame.
  • the real-time underwater particle detection system can image fine particles contained in water in the same manner by forming a thin water film instead of bubbles.
  • FIG. 13(a) is a schematic diagram of a method of generating a water film using a ring-shaped frame instead of a bubble
  • FIG. 13(b) is an image of a liquid film containing fine particles.
  • FIG. 13(b) when particles having a particle size of 2.0 ⁇ m were used, black spots were observed in the area indicated by a square.
  • a spot image photographed with different particle sizes and concentrations is shown in FIG. 13(c). As a result, it was found that the size and number of spots differed according to the size and concentration of the particles.

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  • Food Science & Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Investigating Or Analysing Materials By Optical Means (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

La présente invention concerne un système de détection de particules sous-marines en temps réel au moyen de bulles. Le système de détection de particules sous-marines en temps réel au moyen de bulles selon la présente invention peut analyser quantitativement des bactéries en temps réel par l'intermédiaire d'une visualisation par génération de bulles et peut analyser les propriétés de surface et les types de bactéries en temps réel par simulation. Ce système de détection est ainsi avantageux en ce qu'il présente un faible coût et un temps réduit de détection et en ce qu'il permet une miniaturisation et une modularisation du système. De plus, la quantité de bactéries mesurées par le système de détection de particules sous-marines en temps réel au moyen de bulles selon la présente invention peut être utilisée comme information pour prédire des concentrations de bactéries dans l'eau et dans des aérosols générés lors de l'éclatement des bulles. Ce système peut en outre être utilisé pour déterminer des types de bactéries par analyse d'image à l'aide de propriétés de surface de bactéries, prédiction de trait simulé par ordinateur et analyse statistique.
PCT/KR2020/002548 2019-02-21 2020-02-21 Système de détection de particules sous-marines en temps réel au moyen de bulles WO2020171652A2 (fr)

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EP4053534A1 (fr) * 2021-03-02 2022-09-07 Damann, Volker Procédé et embarcation permettant de localiser des zones d'un plan d'eau comportant une concentration augmentée de particules

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CN114818519B (zh) * 2022-06-30 2022-10-11 湖南工商大学 预测发泡材料气泡破裂的方法、系统及计算机可读介质

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KR20060025792A (ko) * 2004-09-17 2006-03-22 한국표준과학연구원 세균 수 검출이 가능한 수질 측정 시스템 및 이를 위한디바이스 형성 방법
KR100923461B1 (ko) * 2007-10-31 2009-10-27 전자부품연구원 마이크로 플레이트 시료분석 장치
KR101792787B1 (ko) * 2016-04-27 2017-11-02 한국기계연구원 미세기포 분석 시스템 및 방법
KR101957693B1 (ko) * 2016-08-22 2019-03-14 청주대학교 산학협력단 미세기포를 이용한 수중 계면활성제 농도 측정 장치 및 방법
JP6888289B2 (ja) * 2016-12-12 2021-06-16 株式会社リコー 液滴形成装置、液滴形成方法、及び分注装置

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP4053534A1 (fr) * 2021-03-02 2022-09-07 Damann, Volker Procédé et embarcation permettant de localiser des zones d'un plan d'eau comportant une concentration augmentée de particules
WO2022184778A1 (fr) * 2021-03-02 2022-09-09 Damann, Volker Procédé et véhicule nautique pour la localisation de régions d'une masse d'eau qui présentent une concentration de particules augmentée

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KR102279585B1 (ko) 2021-07-21
KR102279585B9 (ko) 2022-12-27
WO2020171652A3 (fr) 2020-10-15

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