WO2020157789A1 - Dispositif d'analyse - Google Patents

Dispositif d'analyse Download PDF

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Publication number
WO2020157789A1
WO2020157789A1 PCT/JP2019/002654 JP2019002654W WO2020157789A1 WO 2020157789 A1 WO2020157789 A1 WO 2020157789A1 JP 2019002654 W JP2019002654 W JP 2019002654W WO 2020157789 A1 WO2020157789 A1 WO 2020157789A1
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WO
WIPO (PCT)
Prior art keywords
liquid
flow path
path member
measurement
unit
Prior art date
Application number
PCT/JP2019/002654
Other languages
English (en)
Japanese (ja)
Inventor
佃　康郎
崇英 平松
俊郎 木村
Original Assignee
株式会社島津製作所
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Application filed by 株式会社島津製作所 filed Critical 株式会社島津製作所
Priority to PCT/JP2019/002654 priority Critical patent/WO2020157789A1/fr
Publication of WO2020157789A1 publication Critical patent/WO2020157789A1/fr

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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/10—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices

Definitions

  • the present disclosure relates to an analysis device.
  • Patent Document 1 Japanese Patent No. 4645739
  • Patent Document 2 Japanese Patent No. 4853518
  • the measurement device It is also possible to install the measurement device with the liquid sample held in the pipette and perform the measurement without dropping the liquid sample collected with a pipette etc. on the sample stage, but the liquid sample is placed on the optical path of the measurement device. It is difficult to accurately arrange the parts of. Furthermore, when a small amount of liquid sample of 1 ⁇ L or less is collected, the liquid sample is retained near the tip of the pipette. Since the liquid sample held near the tip of the pipette comes into direct contact with the external space open to the outside air, the solvent of the sample is likely to volatilize. It becomes difficult to analyze the liquid sample.
  • the present disclosure has been made in view of the above problems, and an object of the present disclosure is to provide an analyzer that can accurately analyze a trace amount of liquid that is even less than 1 ⁇ L.
  • the analysis device of the present disclosure includes a liquid collecting tool configured to collect a very small amount of liquid, and a device main body part having an installation part for detachably installing the liquid collecting tool.
  • the apparatus main body section internally includes a measurement unit for analyzing the liquid collected by the liquid collecting tool.
  • the liquid collecting tool has one end and the other end, and is provided so as to communicate with a flow path member in which a flow path through which the liquid can flow is formed and from the one end side of the flow path member to the flow path.
  • the device body is provided with an insertion hole for inserting the flow path member into the device body. With the liquid collecting tool installed in the installation section, the other end of the flow path member is inserted into the apparatus main body section through the insertion hole.
  • the device body further includes a moving mechanism that moves the measurement unit along the flow path member inserted inside the device body.
  • a small amount of liquid is sucked into the flow path member that constitutes a part of the liquid collecting tool, and the air is formed between the air layers formed on both end sides of the flow path. It can hold a very small amount of liquid. This makes it possible to hold a small amount of liquid at a position apart from the other end of the flow path that is in direct contact with the outside air, and suppress evaporation of the liquid (especially highly volatile solvent). As a result, it is possible to suppress fluctuations in the volume and concentration of the liquid held in the flow path member.
  • the position where the small amount of liquid is held may vary within the flow path.
  • the liquid sampling tool is installed in the installation part in a state where the other end of the flow path member is inserted into the apparatus main body part in which the measurement unit is provided, the liquid is held at a position displaced from the target position. Things can happen.
  • the liquid sample can be measured by the measurement unit in a state in which the fluctuation of the volume and the concentration of the liquid is suppressed, and as a result, a minute amount of the liquid sample can be accurately measured.
  • the measurement unit the irradiation unit for irradiating the measurement light toward the flow path member inserted into the inside of the apparatus main body
  • the measurement light is irradiated
  • One of the flow path member which forms a passage for at least the measurement light and which receives the light from the flow path member, and inserts the measurement light emitted from the irradiation unit into the inside of the apparatus main body. It is preferable to include an optical system for guiding to a partial region.
  • the light receiving section can be composed of a photodiode or an image sensor.
  • the moving mechanism includes the irradiation unit so that the liquid held in the flow path member inserted inside the device main body is irradiated with the measurement light. It is preferable that the measurement unit is moved along the flow path member while being irradiated with the measurement light. In this case, it is preferable that the measurement unit measures the optical characteristic distribution including the optical characteristics of the liquid in the range of the flow path member irradiated with the measurement light while the measurement unit is moving.
  • the analysis device of the present disclosure preferably specifies the position of the liquid based on the optical characteristic distribution.
  • the above-mentioned optical characteristic distribution includes the optical characteristic obtained from the portion of the flow path member where the liquid is not held and the optical characteristic obtained from the portion where the liquid is held.
  • the position (range) where the liquid exists can be specified from the difference in each optical characteristic.
  • the analysis device of the present disclosure may include a storage unit that stores the signal obtained by the light receiving unit.
  • the signal obtained by the light receiving unit is stored in the storage unit at a position where the measurement light is not applied to the liquid.
  • the recording unit records the signal about the optical characteristic distribution measured at the position where the liquid is not held and the liquid is not irradiated with the measurement. It Therefore, it is possible to prevent the measurement from being completed while the optical characteristics of the liquid are being measured, and to reliably measure the optical characteristics of the liquid.
  • the analyzer of the present disclosure may further include a data processing unit that calculates the concentration of the specific component contained in the liquid based on the optical characteristic distribution.
  • the concentration of the characteristic component contained in the liquid can be calculated from the optical characteristic distribution including the measured optical characteristic of the liquid.
  • the measurement unit is arranged on the optical path of the measurement light from the irradiation unit toward the flow path member, and selectively transmits the light in the first wavelength band. It may include a wavelength selection element.
  • the wavelength of the measurement light with which the liquid is irradiated can be set to a desired wavelength band effective for measurement.
  • the measurement unit is arranged on the optical path of the light traveling from the flow path member to the light receiving unit, and emits light in the second wavelength band different from the first wavelength band. It may include a second wavelength selection element that selectively passes through.
  • the wavelength of the light received by the light receiving unit can be set to the second wavelength band different from the first wavelength band included in the measurement light with which the liquid is irradiated. Accordingly, it is possible to receive light having a desired wavelength band in which measurement light is not mixed, and it is possible to improve measurement accuracy.
  • the optical system may include a diaphragm portion provided with an opening that defines an irradiation region of the measurement light with which the flow path member is irradiated.
  • the opening width of the opening along the flow path is preferably shorter than the length of the liquid held in the flow path member along the flow path.
  • the device body may be provided with a guide that extends in the insertion direction of the flow path member and guides the insertion of the flow path member.
  • the flow path member can be guided to the measurement position on the optical path of the measurement light.
  • the guide is configured such that the flow path member can be inserted therein, and the first cylinder is arranged side by side in the insertion direction so that the cylinder axis is parallel to the insertion direction. It may include a tubular portion and a second tubular portion. In this case, a gap is formed between the first tubular portion and the second tubular portion in the insertion direction, and the measurement light is emitted to the flow path member through the gap. Areas may be defined.
  • the number of parts can be reduced by configuring as described above and providing the guide for guiding the insertion of the flow path member with a throttle function.
  • the irradiation unit may be a light emitting diode (LED: Light Emitting Diode).
  • LED Light Emitting Diode
  • the liquid may include a liquid sample containing nucleic acid and an additive for fluorescently labeling the nucleic acid.
  • the operation of mixing the sample and the reagent on the analyzer side can be omitted by collecting and measuring the premixed mixed solution.
  • an analysis device capable of performing accurate analysis regardless of the position where a trace amount of liquid held in a flow path member exists.
  • FIG. 3 is a perspective view showing the appearance of the analyzer according to the first embodiment.
  • 1 is a schematic configuration diagram showing a configuration of an analysis device according to a first embodiment.
  • FIG. 3 is a schematic diagram showing a measuring unit and a moving mechanism included in the analyzer according to the first embodiment.
  • FIG. 3 is a schematic plan view showing the measurement unit according to the first embodiment.
  • FIG. 5 is a schematic side view of the measurement unit viewed from the direction of arrow V shown in FIG. 4.
  • FIG. 6 is a diagram showing movement of measurement light when moving and measuring the measurement unit according to the first embodiment.
  • FIG. 3 is a diagram showing an optical characteristic distribution measured by the measuring unit according to the first embodiment.
  • FIG. 9 is a diagram showing a measurement unit and a moving mechanism in the analysis device according to the second embodiment. It is a schematic sectional drawing which shows the guide shown by the measurement unit shown in FIG.
  • FIG. 1 is a perspective view showing the external appearance of the analyzer according to the first embodiment.
  • FIG. 2 is a schematic configuration diagram showing the configuration of the analyzer according to the first embodiment. An analyzer 1 according to the first embodiment will be described with reference to FIGS. 1 and 2.
  • the analyzer 1 is, for example, an apparatus that analyzes the quantification and/or concentration of nucleic acids such as RNA and DNA involved in protein synthesis.
  • the analysis device 1 includes a liquid sampling tool 10, a device body portion 20, and a data processing device 30.
  • the liquid collecting tool 10 is configured to collect a small amount of liquid L of about 1 nL to 100 nL.
  • the liquid L collected by the liquid collecting tool 10 is, for example, a mixed liquid in which a sample containing a nucleic acid and a fluorescent reagent for fluorescently labeling the nucleic acid are mixed. By collecting and measuring the premixed mixed solution, the operation of mixing the sample and the reagent on the analyzer 1 side can be omitted.
  • Fluorescent reagents are appropriately selected according to the sample to be analyzed.
  • the fluorescent reagent one containing a fluorescent dye such as PicoGreen (registered trademark) and SYBR Green (registered trademark) can be used.
  • the liquid collecting tool 10 includes a main body portion 11, a flow channel member 12, a flow channel member mounting portion 13, and a suction mechanism 14.
  • the main body portion 11 accommodates therein the suction mechanism 14, the flow path member attachment portion 13, and a part of the flow path member 12.
  • the flow path member 12 has one end 12a and the other end 12b.
  • the flow path member 12 is formed with a flow path 12c through which the liquid L can flow.
  • the flow path 12c is formed from one end 12a of the flow path member 12 to the other end 12b.
  • the flow path member 12 is provided, for example, in a linear shape.
  • the flow path member 12 is composed of a light-transmissive tubular member.
  • the inner diameter (flow passage diameter) of the tubular member is 2.0 mm or less, for example, about 0.2 mm.
  • the one end 12 a of the flow path member 12 is attached to the flow path member attachment portion 13 inside the main body 11.
  • the flow channel member mounting portion 13 connects the flow channel member 12 and the suction mechanism 14 so that they can communicate with each other.
  • the flow path member mounting portion 13 is provided with a through hole 13a that connects the flow path 12c of the flow path member 12 and the suction mechanism 14 to each other.
  • the suction mechanism 14 is provided so as to communicate with the flow path 12c from the one end 12a side of the flow path member 12.
  • the suction mechanism 14 is a mechanism for sucking the liquid L into the flow path 12c from the other end 12b side of the flow path member 12.
  • the suction mechanism 14 sucks the liquid L so that the liquid L is held in the flow path 12c.
  • the suction mechanism 14 has an actuator provided with a piezoelectric element and a diaphragm driven by the piezoelectric element.
  • the suction mechanism 14 can suck the liquid L so that the liquid L is held in the flow path 12c by increasing or decreasing the pressure in the flow path 12c by the actuator.
  • a piezoelectric element for example, can be used as the piezoelectric element.
  • the liquid collecting tool 10 collects the liquid L so that the liquid L is held between the air layers formed on both ends of the flow path 12c. As a result, it is possible to prevent the liquid L from being retained near the other end 12b of the flow path member 12 that has an opening surface that is in contact with the outside air outside the flow path member 12. As a result, it is possible to prevent the liquid L from evaporating from the opening surface to the outside air, and it is possible to suppress the variation in the concentration of the liquid L held in the flow path member 12.
  • the liquid L is prevented from being held at the one end 12a of the flow path member 12. Therefore, as will be described later, when the channel member 12 is inserted from the insertion hole 22 into the inside of the apparatus main body 20 during measurement, the length of the channel member 12 to be inserted can be shortened.
  • the liquid collecting tool 10 may collect two kinds of liquids of a sample and a fluorescent reagent in order, and mix the collected two kinds of liquids in the channel.
  • the liquid collecting tool 10 retains the sample and the fluorescent reagent in the flow channel 12c by the suction mechanism 14 and repeatedly pressurizes and depressurizes the flow channel 12c, whereby the sample is collected. And it is preferable to mix the above fluorescent reagents.
  • the mixed liquid can be prepared by controlling the operation of the suction mechanism 14.
  • the operation of the suction mechanism 14 is controlled by, for example, the suction mechanism control unit 23 provided inside the apparatus main body unit 20.
  • the suction mechanism 14 is connected to the suction mechanism control unit 23 by a wire 24.
  • the suction mechanism control unit 23 may be provided in the main body 11 of the liquid sampling tool 10, and in this case, the wiring 24 and the power feeding unit may also be provided in the main body 11.
  • the device body 20 includes a measurement unit 40 and a moving mechanism 50 inside.
  • the measuring unit 40 is a unit for measuring the liquid L collected by the liquid collecting tool 10.
  • the moving mechanism 50 is a mechanism for moving the measurement unit along the flow path member 12 inserted in the apparatus main body 20. The configurations of the measuring unit 40 and the moving mechanism 50 will be described later with reference to FIGS. 3 to 5.
  • the apparatus body 20 is provided with an installation section 21 for detachably installing the liquid collection tool 10. As shown by the broken line in FIG. 1, the liquid collecting tool 10 is detached from the apparatus main body 20 when collecting the liquid L. The liquid collecting tool 10 is installed in the apparatus main body 20 when measuring the collected liquid L.
  • the device body 20 is provided with an insertion hole 22 for inserting the flow path member 12 of the liquid collecting tool 10 into the device body 20.
  • the other end 12 b side of the flow path member 12 is inserted into the insertion hole 22 and the main body part 11 is fixed to the installation part 21.
  • the other end 12b side of the flow path member 12 is inserted into the inside of the apparatus main body section 20.
  • the liquid L held in the flow path member 12 is arranged at the measurement position in the measurement unit 40.
  • the data processing device 30 is equipped with a predetermined control program for executing various types of control and processing, and is connected to the device body 20.
  • the data processing device 30 includes a display unit 31, an operation unit 32, a control unit 33, and a storage unit 36.
  • the display unit 31 displays information for measurement, measurement results, and the like.
  • the operation unit 32 is for setting various parameters related to measurement and instructing various processes.
  • the control unit 33 includes a measurement control unit 34 and a data processing unit 35.
  • the measurement control unit 34 controls the operation of the measurement unit 40 and the operation of the moving mechanism 50.
  • the data processing unit 35 executes various kinds of arithmetic processing for analyzing the liquid L based on the signal received from the measurement unit 40.
  • the storage unit 36 stores the signal received from the measurement unit 40, the processing result of the data processing unit 35, and the like.
  • the storage unit 36 stores the signal received from the measurement unit, the execution result of the data processing unit 35, and the like.
  • the execution result and the like stored in the storage unit 36 may be configured to be retrieved from the data processing device 30 in order to perform processing and data management in an external computer.
  • FIG. 3 is a schematic diagram showing a measurement unit and a moving mechanism included in the analyzer according to the first embodiment.
  • the moving mechanism 50 according to the first embodiment will be described with reference to FIG.
  • the moving mechanism 50 includes a base portion 51, a moving body 52, and a guide rail 53.
  • the base portion 51 holds the measurement unit 40.
  • the base portion 51 is provided so as not to interfere with the flow path member 12 inserted in the apparatus body portion 20.
  • the base portion 51 is provided with a through hole, and the flow path member 12 is inserted into the apparatus main body portion 20 so as to penetrate the through hole.
  • the base portion 51 is fixed to the moving body 52.
  • the moving body 52 is provided so as to be movable along the guide rail 53, as shown by an arrow DR1.
  • the moving body 52 is moved by a drive source such as a motor.
  • the guide rail 53 extends in a direction parallel to the inserted flow path member 12. That is, the guide rail 53 extends along the insertion direction of the flow path member 12.
  • the insertion direction of the flow path member 12 is a direction perpendicular to the opening surface of the insertion hole 22, and is, for example, the vertical direction.
  • the base portion 51 fixed to the moving body 52 also moves along the guide rail 53.
  • the measurement unit 40 held by the base portion 51 moves along the inserted flow path member 12.
  • FIG. 4 is a schematic plan view showing the measurement unit according to the first embodiment.
  • FIG. 5 is a schematic side view of the measurement unit as seen from the direction of arrow V shown in FIG.
  • the measurement unit 40 will be described with reference to FIGS. 4 and 5.
  • the measurement unit 40 includes an irradiation unit 41, a light receiving unit 42, an optical system 43, a first wavelength selection element 44, a second wavelength selection element 45, and a diaphragm unit 46.
  • the irradiation unit 41 irradiates the measurement light MB toward the liquid L held in the flow path member 12.
  • the irradiation unit 41 emits light including a wavelength band that excites the fluorescent dye contained in the liquid L (mixed liquid) to be analyzed.
  • the irradiation unit 41 emits blue visible light having a main wavelength in the vicinity of 470 nm, for example.
  • an LED can be used as the irradiation unit 41.
  • the measurement light is turned on for a predetermined time before the measurement is started to reach the heat parallel.
  • the measurement light MB emitted from the irradiation unit 41 travels toward the first wavelength selection element 44.
  • the first wavelength selection element 44 is arranged on the optical path of the measurement light MB from the irradiation unit 41 toward the flow path member 12.
  • the first wavelength selection element 44 selectively passes light in the first wavelength band.
  • the first wavelength selection element is, for example, a bandpass filter.
  • the first wavelength band is a wavelength band that excites the fluorescent dye contained in the liquid L (mixed liquid) to be analyzed.
  • the measurement light MB that has passed through the first wavelength selection element 44 is guided by the optical system 43.
  • the optical system 43 forms at least a passage for the measurement light MB and guides the measurement light MB emitted from the irradiation unit 41 to the flow path member 12.
  • the optical system 43 includes, for example, a condenser lens such as a ball lens, and guides the measurement light MB passing through the first wavelength selection element 44 to the flow path member 12 while condensing the measurement light MB.
  • the measurement light MB condensed by the optical system 43 has its optical range limited by the diaphragm 46 before reaching the flow path member 12.
  • the throttle portion 46 is arranged near the flow path member 12 inserted inside the apparatus main body portion 20.
  • the narrowed portion 46 has, for example, a plate shape.
  • the diaphragm 46 has an opening 46a that defines an irradiation region of the measurement light MB with which the flow path member is irradiated.
  • the liquid L held in the flow path member 12 extends along the flow path 12c, and the opening width L2 of the opening 46a along the flow path 12c is the flow of the liquid L held in the flow path member 12. It is shorter than the length L1 along the path 12c.
  • the length of the liquid L along the flow path 12c when a small amount of the liquid L is held in the flow path 12c is approximately 2 mm to 3 mm, depending on the flow path diameter of the flow path member 12.
  • the measurement light MB can be applied only to the region on the optical path where the liquid L exists. It will be possible.
  • the measurement light MB has the first wavelength band that excites the fluorescent dye contained in the liquid L. Therefore, when the liquid L held in the flow path member 12 is irradiated with the measurement light MB, fluorescence is emitted from the fluorescent dye excited by the measurement light MB.
  • the light receiving section 42 is arranged at a position rotated about 90 degrees with respect to the irradiation section 41 around the central axis of the flow path member 12.
  • the light receiving unit 42 receives the light from the flow path member 12 irradiated with the measurement light MB.
  • the second wavelength selection element 45 is arranged on the optical path of the light traveling from the flow path member to the light reception section 42, and the light reception section 42 receives the light that has passed through the second wavelength selection element 45.
  • the second wavelength selection element 45 selectively passes light in the second wavelength band different from the first wavelength band.
  • the second wavelength selection element 45 is, for example, a bandpass filter.
  • the second wavelength band is a wavelength range of the fluorescence emitted from the fluorescent dye. Therefore, the measurement light MB is blocked by the second wavelength selection element 45. Accordingly, the light receiving unit 42 can receive light having a desired wavelength band in which the measurement light MB is not mixed, and the measurement accuracy can be improved.
  • the trace amount of the liquid L in the flow channel member 12 is retained between the air layers formed on both end sides of the flow channel 12c, and thus the trace amount retained in the flow channel 12c.
  • the position of the liquid L may vary. In this case, when the liquid sampling tool 10 is installed in the installation section 21 with the other end 12b of the flow path member 12 inserted in the apparatus main body section 20 in which the measurement unit 40 is provided, it is displaced from the target position. It is possible that the liquid is held at the position, that is, the liquid L is not held on the optical path of the measurement light MB.
  • the moving mechanism 50 that moves the measurement unit 40 is provided, and the measurement unit is moved along the flow path member 12 that is inserted into the inside of the apparatus main body portion 20. It is possible to reliably measure the liquid L held in.
  • FIG. 6 is a diagram showing the movement of the measurement light MB when the measurement unit according to the first embodiment is moved to perform measurement. The movement of the measurement light MB when the measurement unit 40 is moved to perform measurement will be described with reference to FIG.
  • the moving mechanism 50 irradiates the measurement light MB from the irradiation unit 41 so that the liquid L held in the flow path member 12 inserted inside the apparatus main body 20 is irradiated with the measurement light MB. 40 is moved along the flow path member 12.
  • the measurement light MB moves along the flow path member 12 as shown by the arrow AR1 in FIG. 6, and includes both a portion where the liquid L is not held and a portion where the liquid L is held. Is irradiated.
  • the measurement unit 40 measures the optical characteristic distribution including the optical characteristic of the liquid L in the range of the flow path member 12 irradiated with the measurement light MB during movement.
  • the optical characteristic for example, light intensity is measured.
  • FIG. 7 is a diagram showing an optical characteristic distribution measured by the measurement unit according to the first embodiment.
  • the optical characteristic distribution measured by the measurement unit according to the first embodiment will be described with reference to FIG. 7.
  • the liquid L held in the flow path member 12 is irradiated with the measurement light MB, the liquid L and a part of the measurement light MB whose optical path is changed by the flow path member, and the liquid L The emitted fluorescence goes toward the light receiving unit 42.
  • the measurement light MB is irradiated to the portion of the flow path member 12 in which the liquid L is not held, part of the measurement light MB whose optical path has been changed by the flow path member 12 is directed to the light receiving unit 42.
  • the light traveling toward the light receiving unit 42 passes through the second wavelength selection element 45, so that the light blocked by the measurement light MB is introduced into the light receiving unit 42.
  • the optical characteristics obtained from that portion become large.
  • the optical characteristic obtained from that portion is small and the optical characteristic distribution is flat.
  • the measured optical characteristic distribution is transmitted to the storage unit 36 as a signal obtained by the light receiving unit, and is stored in association with the moving distance of the moving mechanism 50.
  • the optical characteristics of the liquid held in the movable range of the moving mechanism 50 can be measured.
  • a signal regarding the measured optical characteristic distribution is transmitted from the light receiving unit 42 to the data processing unit 35.
  • the data processing unit 35 calculates the concentration of the specific component (nucleic acid) contained in the liquid L from the above optical characteristic distribution and the optical characteristic obtained by measuring the liquid having a known specific concentration. Further, the data processing unit 35 specifies the position of the liquid L based on the value Em at which the optical characteristic has a peak and the moving distance. In this case, the measurement unit 40 may be moved so that the specified position of the liquid L is irradiated with the measurement light MB, and the measurement may be performed again. As a result, the measurement light MB is surely applied to the liquid L, and the measurement at the position where the light from the liquid L is guided to the light receiving unit 42 becomes possible. Furthermore, it is possible to perform evaluation by the peak value Em of the light intensity, or evaluation by the product of the light intensity and the moving distance, and by using the calculated optical characteristic value per volume L of the liquid, it is possible to perform analysis with higher accuracy. Become.
  • the variation rate of the optical characteristics of the light received from the flow path member 12 in the portion where the liquid L is not held is calculated, and the variation rate is multiplied by the optical characteristics of the liquid. You may. In this case, by measuring the optical characteristics of a portion (particularly the side near the one end 12a of the flow path member 12) where the liquid L does not adhere even when sucked into the flow path member 12, the optical characteristics are measured. It is possible to correct the fluctuation factors of the optical characteristics that occur.
  • the suction mechanism 14 is driven by using the liquid collecting tool 10 including the flow channel member 12 in which the flow channel 12c is formed as described above and the suction mechanism 14. It is possible to hold a small amount of the liquid L between the air layers formed on both ends of the flow path 12c while sucking the small amount of the liquid L into the flow path member 12. This makes it possible to hold a small amount of the liquid L at a position apart from the other end of the flow path 12c that is in direct contact with the outside air opened to the external space, and prevent the liquid L from volatilizing. As a result, fluctuations in the concentration of the liquid L held in the flow path member 12 can be suppressed.
  • the liquid sampling tool 10 is installed in a state where the other end of the flow path member 12 is inserted into the apparatus main body 20 in which the measuring unit 40 is provided. Even when the liquid L is held at a position deviated from the target position when the measurement unit 40 is installed at 21, the measurement unit 40 is moved along the flow path member 12 inserted inside the apparatus main body 20. Thereby, the liquid L held in the flow path member 12 can be measured reliably. As a result, the optical characteristics of the liquid L can be measured while suppressing changes in the volume and concentration of the liquid L, and as a result, a very small amount of the liquid L can be accurately measured.
  • FIG. 8 is a diagram showing a measuring unit and a moving mechanism in the analyzer according to the second embodiment. Note that FIG. 8 illustrates the measurement unit 40A and the moving mechanism 50A when the irradiation unit 41 is viewed from the front side, and for convenience, the first wavelength selection element and the optical system included in the measurement unit 40A are omitted. There is.
  • the analyzer according to the second embodiment will be described with reference to FIG.
  • the analyzer according to the second embodiment is different from the analyzer according to the first embodiment in the configuration of the moving mechanism 50A and the configuration of the measurement unit 40A, and other configurations are the same. , Is almost the same.
  • the measurement unit 40A differs from the measurement unit 40 according to the first embodiment in that a guide 47 is provided.
  • the guide 47 extends in the insertion direction of the flow path member 12 and guides the insertion of the flow path member 12.
  • the guide 47 has a first tubular portion 471 and a second tubular portion 472.
  • the moving mechanism 50A is different from the moving mechanism 50 according to the first embodiment in the configuration of the base portion 51A. Other configurations are almost the same.
  • the base portion 51A includes a first plate portion 511 and a second plate portion 512 that are arranged apart from each other in the insertion direction (vertical direction) of the flow path member 12.
  • the first plate portion 511 and the second plate portion 512 are integrally fixed so as not to affect the measurement by the measurement unit 40A.
  • the first plate portion 511 and the second plate portion 512 are provided with a through hole 511a and a through hole 512a.
  • the guide 47 is held by the through hole 511a and the through hole 512a.
  • the first tubular portion 471 is inserted and held in the through hole 511a
  • the second tubular portion 472 is inserted and held in the through hole 512a.
  • FIG. 9 is a schematic sectional view showing a guide included in the measuring unit shown in FIG.
  • the above-described first tubular portion 471 and second tubular portion 472 included in the guide 47 will be described with reference to FIG. 9.
  • the first tubular portion 471 and the second tubular portion 472 are arranged side by side in the insertion direction so that the tubular axes are parallel to the insertion direction of the flow path member 12.
  • the first tubular portion 471 is arranged such that the flow path member 12 is inserted before the second tubular portion 472.
  • the first tubular portion 471 has a guiding portion 471a for guiding the other end 12b of the flow path member 12 into the guide path 471b.
  • the guide portion 471a is formed so that the inner diameter becomes smaller toward the guide path 471b.
  • the guide path 471b is linearly formed along the insertion direction.
  • the second tubular portion 472 has a guide passage 472b facing the guide passage 471b of the first tubular portion 471.
  • the other end 12b of the flow path member 12 guided into the guiding portion 471a is further inserted to enter the guide path 472b through the guide path 471b.
  • the diameter of the guide passage 472b is larger than the diameter of the guide passage 471a, thereby preventing the other end 12b of the flow path member 12 from interfering with each other when entering the second tubular portion 472.
  • a gap GP is formed between the first tubular portion 471 and the second tubular portion 472 in the insertion direction.
  • the size of the gap GP in the insertion direction is shorter than the length of the liquid L held in the flow path member 12 along the flow path 12c.
  • the gap corresponds to the opening 46a of the first embodiment, and the gap defines the irradiation area with which the flow path member is irradiated. Further, the light from the flow path member 12 irradiated with the measurement light MB travels from the gap GP to the light receiving section 42.
  • the analysis device according to the second embodiment can obtain substantially the same effect as that of the first embodiment.
  • the provision of the guide 47 facilitates disposing the flow path member 12 at the measurement position on the optical path of the measurement light MB.
  • the number of parts can be reduced by forming the narrowed portion with the guide 47.
  • the guide 47 is configured by two tubular portions that are separated from each other has been described as an example, but the present invention is not limited to this, and the guide 47 is configured by one tubular portion. It may be configured. In this case, it is preferable that the peripheral wall of the tubular portion be provided with an opening that defines the irradiation area of the measurement light MB and a window that allows the light from the irradiated flow path member to pass through.
  • the present invention is not limited to this.
  • the light receiving section may be arranged on the optical path through which the measurement light MB passes through the sample. Also in this case, the light receiving section can receive the light from the liquid L (the measurement light MB transmitted through the liquid L), and measures the transmittance of the liquid L and the absorbance of the liquid L at the wavelength of the measurement light MB ( Absorbance analysis).

Abstract

L'invention concerne un dispositif d'analyse (1) comprenant un outil de collecte de liquide (10) et une partie corps de dispositif (20) possédant une partie montage permettant de monter amovible l'outil de collecte de liquide (10). La partie corps de dispositif (20) comprend une unité de mesure (40) sur l'intérieur de la partie corps de dispositif (20). L'outil de collecte de liquide (10) comprend un élément de trajet d'écoulement (12) possédant un trajet d'écoulement (12c) formé à l'intérieur de ce dernier et un mécanisme d'aspiration (23) permettant d'aspirer un liquide dans le trajet d'écoulement (12c). Le mécanisme d'aspiration (23) aspire le liquide de telle sorte que ce dernier soit maintenu entre des couches d'air formées aux deux extrémités du trajet d'écoulement (12c). Lorsque l'outil de collecte de liquide (10) est monté dans la partie montage, l'élément de trajet d'écoulement (12) est inséré à l'intérieur de la partie corps de dispositif (20) depuis un trou d'insertion. La partie corps de dispositif (20) comprend en outre un mécanisme de déplacement (50) permettant de déplacer l'unité de mesure (40) le long de l'élément de trajet d'écoulement (12) inséré à l'intérieur de la partie corps de dispositif (20).
PCT/JP2019/002654 2019-01-28 2019-01-28 Dispositif d'analyse WO2020157789A1 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
PCT/JP2019/002654 WO2020157789A1 (fr) 2019-01-28 2019-01-28 Dispositif d'analyse

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PCT/JP2019/002654 WO2020157789A1 (fr) 2019-01-28 2019-01-28 Dispositif d'analyse

Publications (1)

Publication Number Publication Date
WO2020157789A1 true WO2020157789A1 (fr) 2020-08-06

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WO (1) WO2020157789A1 (fr)

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS62144073A (ja) * 1985-12-18 1987-06-27 Hitachi Ltd 分注装置
JP2000235037A (ja) * 1999-02-16 2000-08-29 Hitachi Ltd 試料分析装置
US20050169816A1 (en) * 2003-12-15 2005-08-04 Kirshner Brian M. Automated oligomer synthesis
WO2005106433A1 (fr) * 2004-04-30 2005-11-10 Precision System Science Co., Ltd. Lecteur d’informations optiques
JP2006349638A (ja) * 2005-06-20 2006-12-28 Fujifilm Holdings Corp 微量液体の均一化方法及び装置
JP2016524124A (ja) * 2013-03-15 2016-08-12 ハイコア バイオメディカル インコーポレイテッド サンプルの発光及び蛍光測定を行うための装置及び関連する方法

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS62144073A (ja) * 1985-12-18 1987-06-27 Hitachi Ltd 分注装置
JP2000235037A (ja) * 1999-02-16 2000-08-29 Hitachi Ltd 試料分析装置
US20050169816A1 (en) * 2003-12-15 2005-08-04 Kirshner Brian M. Automated oligomer synthesis
WO2005106433A1 (fr) * 2004-04-30 2005-11-10 Precision System Science Co., Ltd. Lecteur d’informations optiques
JP2006349638A (ja) * 2005-06-20 2006-12-28 Fujifilm Holdings Corp 微量液体の均一化方法及び装置
JP2016524124A (ja) * 2013-03-15 2016-08-12 ハイコア バイオメディカル インコーポレイテッド サンプルの発光及び蛍光測定を行うための装置及び関連する方法

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