WO2020111367A1 - Oenanthe javanica juice having increased intestinal uptake and preparation method therefor - Google Patents

Oenanthe javanica juice having increased intestinal uptake and preparation method therefor Download PDF

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WO2020111367A1
WO2020111367A1 PCT/KR2018/015841 KR2018015841W WO2020111367A1 WO 2020111367 A1 WO2020111367 A1 WO 2020111367A1 KR 2018015841 W KR2018015841 W KR 2018015841W WO 2020111367 A1 WO2020111367 A1 WO 2020111367A1
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juice
buttercup
apple
lemon
low temperature
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PCT/KR2018/015841
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French (fr)
Korean (ko)
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함대식
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농업회사법인 (주)배려
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    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L2/00Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
    • A23L2/02Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation containing fruit or vegetable juices
    • A23L2/04Extraction of juices
    • A23L2/06Extraction of juices from citrus fruits
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L3/00Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs
    • A23L3/32Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs by treatment with electric currents without heating effect
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A23L33/105Plant extracts, their artificial duplicates or their derivatives

Definitions

  • the present invention relates to a buttercup juice having a high intestinal absorption rate and a method for manufacturing the same, and more specifically, a living yeast and an enzyme decompose cellulose through low temperature aging, and a natural apple sugar combines with a buttercup molecule to increase the intestinal absorption rate. And a method of manufacturing the same.
  • Buttercup is a perennial plant with a mountain type, and is a native plant in Korea that grows with long stems. It is a leafy stem vegetable that grows mainly in spring and autumn in the fields or streams, and is characterized by its unique aroma and soft texture. Buttercup is an alkaline food that prevents and purifies the acidification of blood, and has a variety of nutrients, so it has an excellent detoxification effect and has an excellent effect of discharging heavy metals and various toxins from the body.
  • buttercup is rich in vitamins and minerals, so it is a good ingredient to neutralize the constitution that has changed to acid due to a high-fat diet.
  • it since it contains a lot of potassium, it helps to release harmful components such as heavy metals and sodium in the body, and isorhamnetin and persicarin, essential oil components of buttercup, suppress inflammation and decompose alcohol to relieve hangover It also has a good effect.
  • it is necessary to pay attention to the consumption of buttercups because it stimulates the digestive system such as the stomach with strong scent.
  • KR10-2016-0104131 relates to a method for manufacturing buttercup essence and buttercup drinks, The sugar is mixed with fermentation, fermented and aged to make a essence, and using it, a method for preparing a buttercup beverage
  • KR10-1245191 is a method for preparing a buttercup fermentation liquid, a method of natural fermentation by mixing buttercup and brown sugar or oligosaccharides, etc. This is disclosed.
  • the present inventors completed the present invention by developing a buttercup juice that mixes fruit juice after low temperature aging, improves the strong aroma of buttercup, and increases digestion and absorption rate in the intestine through low temperature aging.
  • An object of the present invention is to provide a buttercup juice drink with increased digestive absorption rate in the intestine through low temperature aging.
  • Another object of the present invention is to provide a method for preparing a buttercup juice, in which the living yeast and enzyme decompose cellulose through low temperature aging, and natural apple sugar is combined with a buttercup molecule to increase intestinal absorption.
  • the present invention is a first step of washing the buttercup after juice
  • a third step of mixing apple juice and lemon juice in a low-temperature aged buttercup juice is a third step of mixing apple juice and lemon juice in a low-temperature aged buttercup juice.
  • buttercup juice beverage with improved digestive absorption rate in the intestine, including a low temperature aging liquid of a buttercup juice, apple juice, and lemon juice.
  • the present invention can reduce the aroma peculiar to the buttercup through a process of aging the buttercup and aging it at a low temperature when manufacturing the buttercup juice, and can make a buttercup juice that can be easily consumed by utilizing a soft texture.
  • live yeast and enzymes decompose cellulose, and natural apple sugar can be combined with buttercup molecules to improve the absorption rate in the intestine, and natural fruit sugar is used to produce juice with increased absorption of good nutrients of buttercup. can do.
  • 1 is a buttercup manufactured according to the present invention.
  • the present invention is a first step of washing the buttercup after juice
  • the buttercup is washed and then juiced (first step).
  • the first step can be juiced using a juicer.
  • the second step is preferably aged at a low temperature in a storage room of 0 to 15°C, and more preferably aged at a low temperature of 2 to 10°C.
  • the low-temperature aging is good to proceed for 4 to 6 days
  • the temperature is too low at 0°C or lower, so that the buttercup juice is frozen, and aging is impossible.
  • a sour odor is added to the peculiar scent, which is not good for drinking.
  • apple juice and lemon juice are mixed with the low-temperature aged buttercup juice (the third step).
  • the low temperature aged buttercup juice, apple juice and lemon juice in a ratio of 10 to 30 parts by weight, 68 to 88 parts by weight, and 1 to 3 parts by weight.
  • the apple juice and lemon juice can improve the peculiar flavor of buttercup, and when the blending ratio of apple juice and lemon juice is low, the strong aroma of buttercup is felt so that the drinkers may feel rejection, and the blending ratio of apple juice and lemon juice is high. In this case, the taste of apples, lemons, etc. is felt more than that of buttercups, and the effectiveness of buttercup juice may be lost.
  • natural apple sugar helps to increase the absorption rate in the intestine by combining with the effect molecule of buttercup.
  • the apple juice and the lemon juice are preferably washed after washing the apple and lemon, respectively.
  • a juicer may be used for the juice process.
  • the sterilization is preferably a non-heating low-temperature sterilization of 40 °C or less using a pulsed electric device (Pulsed Electric Field, PEF).
  • PEF Pulsed Electric Field
  • the buttercup drink has a green buttercup color and is easy to package in a liquid form in a glass container.
  • the buttercup juice beverage can improve the intestinal absorption rate through a low temperature aging process.
  • the mixture thus prepared was completed by non-heating low-temperature sterilization using a pulsed electric field (PEF).
  • PEF pulsed electric field
  • the buttercup juice was prepared in the same manner as in ⁇ Example 1>, except that the low temperature aging process was performed for 3 days in a storage at 15°C.
  • the buttercup juice was prepared in the same manner as in ⁇ Example 1>, except that it was aged for 5 days at room temperature (20-25°C).
  • the sensory test agents target 20 adult men and 20 adult women (consisting of 5 men and women in their 20s, 5 in their 30s, 5 in their 40s, and 5 in their 50s). After establishing stable criteria for characterization and taste, sensory testing was conducted. The sensory test items were evaluated on a total of 10 points based on a total of 4 items: taste, aroma, texture, and overall preference.
  • the scoring criteria are as follows.
  • the buttercups produced by ⁇ Examples 1 to 3> had a lot of odor removed and had a savory scent, and the apple juice and lemon juice were properly formulated to improve the flavor, and the low temperature was appropriate during the manufacturing process of the buttercup juice. By maintaining the temperature, it was possible to prepare a buttercup juice preserved as it is without killing the active ingredient in the buttercup.
  • the corresponding juice per each experimental group was consumed one cup of 250 ml per day for one week, and it was carried out by preventing another fruit or fruit juice from being consumed from the 3rd day before the experiment.
  • a total of three items, stool condition, comfort, and stomach comfort level were evaluated on a 10-point scale during the one-week experiment, and the color of the stool was visually observed.
  • the scoring criteria are as follows.
  • Example 1 5.1 Dark green stools 5.0 4.9 Comparative Example 2 5.4 Light green stools 5.2 5.1 Comparative Example 3 6.3 Light green stools 6.0 5.9 Comparative Example 4 6.5 Light green stools 5.8 5.7 Comparative Example 5 6.6 Light green stools 5.7 5.6

Abstract

The present invention relates to Oenanthe javanica juice having good intestinal uptake. More specifically, the present invention can prepare Oenanthe javanica juice in which cellulose is degraded by living yeasts and enzymes through low-temperature aging; Oenanthe javanica molecules are associated with natural apple sugar to increase in intestinal uptake; and the characteristic strong odor of Oenanthe javanica is alleviated by mixing with apple juice and lemon juice.

Description

장내 흡수율을 높인 미나리주스 및 이의 제조방법Buttercup juice with increased intestinal absorption rate and its manufacturing method
본 발명은 장내흡수율을 높인 미나리주스 및 이의 제조방법에 관한 것으로서, 보다 상세하게는 저온 숙성을 통해 살아있는 효모, 효소가 셀룰로오스를 분해하며, 천연 사과 당이 미나리 분자와 결합하여 장내흡수율을 높인 미나리주스 및 이를 제조하는 방법에 관한 것이다.The present invention relates to a buttercup juice having a high intestinal absorption rate and a method for manufacturing the same, and more specifically, a living yeast and an enzyme decompose cellulose through low temperature aging, and a natural apple sugar combines with a buttercup molecule to increase the intestinal absorption rate. And a method of manufacturing the same.
미나리는 산형과의 여러해살이풀로 긴 줄기가 뻗어 번식하는 우리나라 자생식물이다. 주로 봄, 가을에 들판이나 개울가에 자라는 잎줄기채소로 특유의 향긋함과 부드러운 식감이 특징인 재료이다. 미나리는 알카리성 음식으로 혈액의 산성화를 막고 정화하며, 다양한 영양소가 풍부해 해독작용이 뛰어나 체내 중금속이나 각종 독소를 배출하는 탁월한 효과를 가지고 있다. Buttercup is a perennial plant with a mountain type, and is a native plant in Korea that grows with long stems. It is a leafy stem vegetable that grows mainly in spring and autumn in the fields or streams, and is characterized by its unique aroma and soft texture. Buttercup is an alkaline food that prevents and purifies the acidification of blood, and has a variety of nutrients, so it has an excellent detoxification effect and has an excellent effect of discharging heavy metals and various toxins from the body.
구체적으로 미나리는 비타민과 무기질이 풍부하게 함유되어 있어 고지방 식단으로 인해 산성으로 변한 체질을 중화하는데 좋은 식재료이다. 또한, 칼륨이 많이 함유되어 있어 체내의 중금속과 나트륨 등 해로운 성분 배출에 도움이 되며, 미나리의 정유 성분인 이소람네틴(isorhamnetin)과 페르시카린(persicarin)은 염증을 억제하고 알코올을 분해해 숙취 해소에도 좋은 효과를 가지고 있다. 그러나 미나리는 강한 향으로 위 등 소화계를 자극하므로 섭취에 유의할 필요가 있다.Specifically, buttercup is rich in vitamins and minerals, so it is a good ingredient to neutralize the constitution that has changed to acid due to a high-fat diet. In addition, since it contains a lot of potassium, it helps to release harmful components such as heavy metals and sodium in the body, and isorhamnetin and persicarin, essential oil components of buttercup, suppress inflammation and decompose alcohol to relieve hangover It also has a good effect. However, it is necessary to pay attention to the consumption of buttercups because it stimulates the digestive system such as the stomach with strong scent.
따라서 소화계에 자극이 적으며 미나리의 강한 향을 완화시키고 장내 흡수율을 향상시키기 위해 다양한 가공법이 연구되고 있는데, 예를 들어, KR10-2016-0104131에는 미나리 진액 및 미나리 음료의 제조방법에 대한 것으로, 미나리에 설탕을 혼합해 발효 후 숙성시켜 진액을 만들고, 이를 이용하여 미나리 음료를 제조하는 방법, KR10-1245191에는 미나리발효액의 제조방법에 대한 것으로, 미나리와 흑설탕 또는 올리고당을 혼합해 자연 발효시키는 제조방법 등이 개시되어 있다.Therefore, there is little irritation to the digestive system, and various processing methods have been studied to relieve the strong smell of buttercups and improve the absorption rate in the intestine.For example, KR10-2016-0104131 relates to a method for manufacturing buttercup essence and buttercup drinks, The sugar is mixed with fermentation, fermented and aged to make a essence, and using it, a method for preparing a buttercup beverage, KR10-1245191 is a method for preparing a buttercup fermentation liquid, a method of natural fermentation by mixing buttercup and brown sugar or oligosaccharides, etc. This is disclosed.
그러나 위와 같은 선행문헌에 개시된 제조방법으로 제조된 미나리 음료는 설탕 또는 올리고당 등을 포함시킨 후 발효, 숙성시켜 제조하는 방법만이 제시되어 있을 뿐, 미나리주스의 유효 성분이 장내 소화흡수율을 높이는 방법에 대한 것은 연구된 바 없다.However, only the method of manufacturing a buttercup drink prepared by the manufacturing method disclosed in the prior literature as above by including fermentation and aging after including sugar or oligosaccharide is presented, and the active ingredient of the buttercup juice is used to increase the digestive absorption rate in the intestine. Nothing has been studied.
이에 본 발명자들은 저온숙성 후 과일즙을 혼합하여, 미나리의 강한 향을 개선하고 저온숙성을 통해 장내 소화 흡수율을 높이는 미나리주스를 개발함으로써, 본 발명을 완성하였다. Therefore, the present inventors completed the present invention by developing a buttercup juice that mixes fruit juice after low temperature aging, improves the strong aroma of buttercup, and increases digestion and absorption rate in the intestine through low temperature aging.
본 발명의 목적은 저온 숙성을 통해 장내 소화흡수율을 높인 미나리주스 음료를 제공하는 것이다.An object of the present invention is to provide a buttercup juice drink with increased digestive absorption rate in the intestine through low temperature aging.
본 발명의 또 다른 목적은 저온 숙성을 통해 살아있는 효모, 효소가 셀룰로오스를 분해하며, 천연 사과 당이 미나리 분자와 결합하여 장내흡수율을 높인 미나리주스를 제조하는 방법을 제공하는 것이다.Another object of the present invention is to provide a method for preparing a buttercup juice, in which the living yeast and enzyme decompose cellulose through low temperature aging, and natural apple sugar is combined with a buttercup molecule to increase intestinal absorption.
상기 목적을 달성하기 위하여, In order to achieve the above object,
본 발명은 미나리를 세척한 후 착즙하는 제1 단계;The present invention is a first step of washing the buttercup after juice;
착즙액을 0 내지 15℃에서 3일 내지 6일간 저온 숙성하는 제2 단계; 및A second step of aging the juice solution at 0 to 15° C. for 3 to 6 days at low temperature; And
저온 숙성 미나리즙에 사과즙, 레몬즙을 혼합하는 제3 단계;를 포함하는 미나리주스의 제조방법을 제공한다.A third step of mixing apple juice and lemon juice in a low-temperature aged buttercup juice.
또한, 미나리 착즙 저온 숙성액, 사과즙 및 레몬즙을 포함하는 장내 소화흡수율이 향상된 미나리주스 음료를 제공한다.In addition, it provides a buttercup juice beverage with improved digestive absorption rate in the intestine, including a low temperature aging liquid of a buttercup juice, apple juice, and lemon juice.
본 발명은 미나리주스 제조 시, 미나리를 착즙한 뒤 저온에서 숙성하는 과정을 통해 미나리 특유의 향을 줄일 수 있고, 부드러운 식감을 살려 음용이 용이하게 할 수 있는 미나리주스를 제조할 수 있다.The present invention can reduce the aroma peculiar to the buttercup through a process of aging the buttercup and aging it at a low temperature when manufacturing the buttercup juice, and can make a buttercup juice that can be easily consumed by utilizing a soft texture.
또한, 저온 숙성을 통해 살아있는 효모, 효소가 셀룰로오스를 분해하며, 천연 사과 당이 미나리 분자와 결합하여 장내흡수율을 향상시킬 수 있으며, 천연 과일 당을 이용하여 미나리의 좋은 영양소의 흡수율을 높인 주스를 제조할 수 있다.In addition, through low-temperature aging, live yeast and enzymes decompose cellulose, and natural apple sugar can be combined with buttercup molecules to improve the absorption rate in the intestine, and natural fruit sugar is used to produce juice with increased absorption of good nutrients of buttercup. can do.
도 1은 본 발명에 따라 제조된 미나리주스이다.1 is a buttercup manufactured according to the present invention.
이하, 본 발명을 상세하게 설명한다. 본 발명을 설명함에 있어서 관련된 공지 구성 또는 기능에 대한 상세한 설명은 생략할 수 있다.Hereinafter, the present invention will be described in detail. In describing the present invention, detailed descriptions of related well-known structures or functions may be omitted.
본 명세서 및 특허청구범위에 사용된 용어나 단어는 통상적이거나 사전적 의미로 한정되어 해석되지 아니하며, 본 발명의 기술적 사항에 부합하는 의미와 개념으로 해석되어야 한다.The terms or words used in the present specification and claims are not limited to ordinary or dictionary meanings and should not be interpreted, but should be interpreted as meanings and concepts consistent with the technical matters of the present invention.
본 명세서에 기재된 실시예와 도면에 도시된 구성은 본 발명의 바람직한 실시예이며, 본 발명의 기술적 사상을 모두 대변하는 것이 아니므로, 본 출원 시점에서 이들을 대체할 수 있는 다양한 균등물과 변형예들이 있을 수 있다.The embodiments shown in the specification and the drawings shown in the specification are preferred embodiments of the present invention, and do not represent all of the technical spirit of the present invention, and various equivalents and modifications that can replace them at the time of this application It can be.
이하, 본 발명의 미나리주스 제조방법에 대하여 상세히 설명하도록 한다.Hereinafter, a method for manufacturing a buttercup juice of the present invention will be described in detail.
본 발명은 미나리를 세척한 후 착즙하는 제1 단계;The present invention is a first step of washing the buttercup after juice;
착즙액을 0 내지 15℃에서 4일 내지 6일간 저온 숙성하는 제2 단계; 및A second step of aging the juice solution at 0 to 15° C. for 4 to 6 days at low temperature; And
저온 숙성 미나리즙에 사과즙, 레몬즙을 혼합하는 제3 단계;를 포함하는 미나리주스의 제조방법을 포함한다.And a third step of mixing apple juice and lemon juice in a low temperature aged buttercup juice.
먼저, 미나리를 세척한 후 착즙한다(제 1단계).First, the buttercup is washed and then juiced (first step).
상기 제 1단계는 착즙기를 이용해 착즙할 수 있다.The first step can be juiced using a juicer.
다음으로 착즙액을 저온 숙성한다(제2 단계).Next, the juice is aged at low temperature (second step).
상기 제2 단계는 0 내지 15 ℃의 저장고에서 저온 숙성하는 것이 바람직하며, 2내지 10℃의 저장고에서 저온 숙성하는 것이 더욱 바람직하다. The second step is preferably aged at a low temperature in a storage room of 0 to 15°C, and more preferably aged at a low temperature of 2 to 10°C.
또한, 상기 저온 숙성은 4일 내지 6일 간 진행되는 것이 좋다In addition, the low-temperature aging is good to proceed for 4 to 6 days
이 때, 0 ℃ 미만에서는 온도가 너무 낮아 미나리 착즙액이 얼어버려 숙성이 불가능하며, 15 ℃ 초과의 온도에서는 미나리 특유의 향에 시큼한 냄새가 더해져 음용하기에 좋지 않다.At this time, the temperature is too low at 0°C or lower, so that the buttercup juice is frozen, and aging is impossible. At a temperature above 15°C, a sour odor is added to the peculiar scent, which is not good for drinking.
아울러, 0 내지 10 ℃에서 숙성하였을 때 한 달이 넘어도 물성이 변하지 않으며, 부드러운 식감을 살려 맛 또한 감미로워지게 된다.In addition, when aged at 0 to 10 °C, even after a month, the physical properties do not change, and the taste is also sweetened by utilizing a soft texture.
또한, 4일 미만 숙성 시에는 미나리 특유의 향이 강하게 남아있어 음용하기 적합하지 않으며, 7일 이상 숙성 후 음료로 제조하였을 때는 색과 맛이 변하기 쉽다. In addition, when ripening for less than 4 days, the peculiar flavor of buttercup remains strong, making it unsuitable for drinking. When aging for more than 7 days, the color and taste are likely to change.
아울러, 4 내지 6 일간 숙성하였을 때 소화흡수율이 증가하게 된다.In addition, the digestion and absorption rate increases when aged for 4-6 days.
다음으로 저온 숙성 미나리즙에 사과즙 및 레몬즙을 혼합한다(제3 단계).Next, apple juice and lemon juice are mixed with the low-temperature aged buttercup juice (the third step).
상기 제3 단계에서 저온 숙성 미나리즙과 사과즙 및 레몬즙을 10 내지 30 중량부, 68 내지 88 중량부, 1 내지 3 중량부의 비율로 혼합하는 것이 바람직하다.In the third step, it is preferable to mix the low temperature aged buttercup juice, apple juice and lemon juice in a ratio of 10 to 30 parts by weight, 68 to 88 parts by weight, and 1 to 3 parts by weight.
상기 사과즙 및 레몬즙은 미나리 특유의 향을 개선할 수 있으며, 사과즙 및 레몬즙의 배합비율이 낮을 경우 미나리의 강한 향이 많이 느껴져 음용자들이 거부감을 느낄 수 있으며, 사과즙 및 레몬즙의 배합비율이 높을 경우 미나리보다 사과, 레몬 등의 맛이 많이 느껴지며, 미나리주스의 효능을 잃을 수 있다. 아울러 천연 사과 당은 미나리의 영향분자와 결합해 장내흡수율을 높이는데 도움을 준다.The apple juice and lemon juice can improve the peculiar flavor of buttercup, and when the blending ratio of apple juice and lemon juice is low, the strong aroma of buttercup is felt so that the drinkers may feel rejection, and the blending ratio of apple juice and lemon juice is high. In this case, the taste of apples, lemons, etc. is felt more than that of buttercups, and the effectiveness of buttercup juice may be lost. In addition, natural apple sugar helps to increase the absorption rate in the intestine by combining with the effect molecule of buttercup.
상기 제3 단계에서 사과즙 및 레몬즙은 사과 및 레몬을 각각 세척한 후 착즙하는 것이 바람직하다.In the third step, the apple juice and the lemon juice are preferably washed after washing the apple and lemon, respectively.
이때, 착즙 과정은 착즙기를 이용할 수 있다.At this time, a juicer may be used for the juice process.
상기 제3 단계를 통해 제조된 혼합액에 멸균 및 포장하는 단계를 더 포함할 수 있다.It may further include a step of sterilization and packaging in the mixed solution prepared through the third step.
상기 멸균은 펄스전기장치(Pulsed Electric Field, PEF)를 이용하여 40℃ 이하의 비가열 저온 멸균하는 것이 바람직하다. The sterilization is preferably a non-heating low-temperature sterilization of 40 ℃ or less using a pulsed electric device (Pulsed Electric Field, PEF).
이 때, 미나리의 유효성분은 40℃부터 사멸하기 시작하여 60℃가 되면 완전히 사멸되어버리므로, 40℃이하에서 저온 살균하는 것이 바람직하다.At this time, since the effective component of the buttercup starts to die at 40°C and is completely killed when it reaches 60°C, it is preferable to pasteurize at 40°C or less.
상기 미나리주스 음료는 미나리 색인 초록색을 띄며, 액체형태로 유리용기에 포장하기 용이하다.The buttercup drink has a green buttercup color and is easy to package in a liquid form in a glass container.
아울러, 상기 미나리주스 음료는 저온 숙성 과정을 통해 장내흡수율을 향상시킬 수 있는 것을 확인할 수 있었다.In addition, it was confirmed that the buttercup juice beverage can improve the intestinal absorption rate through a low temperature aging process.
이하, 실시예를 통하여 본 발명을 보다 상세히 설명하기로 한다. 이들 실시예는 오로지 본 발명을 보다 구체적으로 설명하기 위한 것으로, 본 발명의 요지에 따라 본 발명의 범위가 이들 실시예에 의해 제한되지 않는다는 것은 당 업계에서 통상의 지식을 가진 자에게 있어서 자명한 것이다.Hereinafter, the present invention will be described in more detail through examples. These examples are only intended to illustrate the present invention in more detail, and it is apparent to those skilled in the art that the scope of the present invention is not limited by these examples according to the gist of the present invention. .
<실시예 1> 미나리주스 제조 1<Example 1> Preparation of buttercup 1
미나리 1 Kg을 깨끗한 물에 세척한 뒤 착즙기를 이용하여 착즙하여 미나리 착즙액을 얻었다. 이후 5 ℃의 저장고에서 5일 동안 저온 숙성 과정을 거친 후, 사과즙 및 레몬즙을 첨가해 잘 섞어 혼합물을 제조하였다. 이때, 저온 숙성 미나리즙 20 중량%, 사과즙 78 중량% 및 레몬즙 2 중량% 비율로 혼합하였다.1 Kg of buttercup was washed with clean water and then juiced using a juicer to obtain a buttercup juice. Thereafter, after a low temperature aging process for 5 days in a storage at 5° C., apple juice and lemon juice were added to mix well to prepare a mixture. At this time, low-temperature ripening buttercup juice was mixed at a ratio of 20% by weight, apple juice by 78% by weight, and lemon juice by 2% by weight.
이렇게 제조한 혼합물을 펄스전기장치(Pulsed Electric Field, PEF)를 이용하여 비가열 저온 멸균하여 완성하였다.The mixture thus prepared was completed by non-heating low-temperature sterilization using a pulsed electric field (PEF).
<실시예 2> 미나리주스 제조 2<Example 2> Preparation of buttercup juice 2
미나리 착즙액을 15℃의 저장고에서 3일 동안 저온 숙성 과정을 거친 것을 제외하고는 상기 <실시예 1>과 동일하게 제조하였다.The buttercup juice was prepared in the same manner as in <Example 1>, except that the low temperature aging process was performed for 3 days in a storage at 15°C.
<실시예 3> 미나리주스 제조 3<Example 3> Preparation of buttercup juice 3
미나리 착즙액 20 중량%, 사과즙 77 중량% 및 레몬즙 3 중량% 혼합한 것을 제외하고는 상기 <실시예 1>과 동일하게 제조하였다.It was prepared in the same manner as in <Example 1> except that 20 wt% of the buttercup juice solution, 77 wt% of apple juice, and 3 wt% of lemon juice were mixed.
<비교예 1> 저온 숙성 과정이 없는 제조<Comparative Example 1> Preparation without low temperature aging process
저온 숙성 과정을 거치지 않은 것을 제외하고는 상기 <실시예 1>과 동일하게 제조하였다.It was prepared in the same manner as in <Example 1> except that the low temperature aging process was not performed.
<비교예 2> 숙성 조건이 다른 제조<Comparative Example 2> Preparation with different aging conditions
미나리 착즙액을 실온(20 ~ 25℃)에서 5일 동안 숙성 과정을 거친 것을 제외하고는 상기 <실시예 1>과 동일하게 제조하였다.The buttercup juice was prepared in the same manner as in <Example 1>, except that it was aged for 5 days at room temperature (20-25°C).
<비교예 3> 배합 조건이 다른 제조<Comparative Example 3> Preparation with different compounding conditions
미나리 착즙액 40 중량%, 사과즙 58 중량% 및 레몬즙 2 중량% 혼합한 것을 사용한 것을 제외하고는 상기 <실시예 1>과 동일하게 제조하였다.It was prepared in the same manner as in <Example 1>, except that 40% by weight of a buttercup juice solution, 58% by weight of apple juice, and 2% by weight of lemon juice were mixed.
<비교예 4> 배합 조건이 다른 제조<Comparative Example 4> Preparation with different compounding conditions
미나리 착즙액 5 중량%, 사과즙 88 중량% 및 레몬즙 7 중량% 혼합한 것을 제외하고는 상기 <실시예 1>과 동일하게 제조하였다.It was prepared in the same manner as in <Example 1> except that 5% by weight of the buttercup juice solution, 88% by weight of apple juice, and 7% by weight of lemon juice were mixed.
<비교예 5> 멸균 조건이 다른 제조<Comparative Example 5> Preparation with different sterilization conditions
고온멸균기를 사용하여 70 ~ 80℃로 멸균한 것을 제외하고는 상기 <실시예 1>과 동일하게 제조하였다. It was prepared in the same manner as in <Example 1>, except that it was sterilized at 70 to 80°C using a high-temperature sterilizer.
<실험예 1> 관능검사<Experimental Example 1> Sensory test
상기 실시예 1 내지 3, 및 비교예 1 내지 5에서 각각 제조한 미나리 주스의 맛, 향 및 전체적인 기호도를 알아보기 위한 관능검사를 실시하였다. Sensory tests were performed to examine the taste, aroma, and overall preference of the buttercup juices prepared in Examples 1 to 3 and Comparative Examples 1 to 5, respectively.
관능검사 요원은 성인 남성 20명과 성인 여성 20명을 대상(남여 각각 20대 5명, 30대 5명, 40대 5명, 50대 5명으로 구성)으로 교육과정을 통하여 평가할 특성에 대한 식별력과 특성, 맛에 대한 안정된 판단기준을 확립한 후 관능검사에 임하였다. 관능검사 항목은 맛, 향, 식감 및 전체적인 기호도의 총 4가지를 10점 만점 기준으로 평가하였다. The sensory test agents target 20 adult men and 20 adult women (consisting of 5 men and women in their 20s, 5 in their 30s, 5 in their 40s, and 5 in their 50s). After establishing stable criteria for characterization and taste, sensory testing was conducted. The sensory test items were evaluated on a total of 10 points based on a total of 4 items: taste, aroma, texture, and overall preference.
채점 기준은 다음과 같다.The scoring criteria are as follows.
아주 좋다 : 10점Very good: 10 points
좋다 : 8점Good: 8 points
보통이다 : 6점Normal: 6 points
나쁘다 : 4점Bad: 4 points
아주 나쁘다 : 2점Very bad: 2 points
그 결과를 표 1에 나타내었다.Table 1 shows the results.
구분division flavor incense 식감Texture 기호도Preference
실시예 1Example 1 9.39.3 9.49.4 9.09.0 9.29.2
실시예 2Example 2 9.19.1 9.29.2 8.98.9 9.19.1
실시예 3Example 3 9.29.2 9.49.4 8.68.6 9.19.1
비교예 1Comparative Example 1 5.25.2 4.84.8 5.95.9 5.35.3
비교예 2Comparative Example 2 5.35.3 4.64.6 6.06.0 4.84.8
비교예 3Comparative Example 3 5.25.2 4.24.2 6.26.2 4.54.5
비교예 4Comparative Example 4 5.05.0 4.34.3 6.26.2 4.74.7
비교에 55 to comparison 5.35.3 4.64.6 6.36.3 5.55.5
맛을 평가해본 결과, <실시예 1 내지 3>에 의해 제조된 미나리 주스는 냄새가 많이 제거되고 구수한 향이 나며, 사과즙 및 레몬즙을 적절히 배합되어 풍미가 향상되었고, 미나리주스의 제조과정 동안 적절한 저온 온도를 유지하여 미나리 내 유효성분을 사멸시키지 않고 그대로 보존한 미나리 주스를 제조할 수 있었다.As a result of evaluating the taste, the buttercups produced by <Examples 1 to 3> had a lot of odor removed and had a savory scent, and the apple juice and lemon juice were properly formulated to improve the flavor, and the low temperature was appropriate during the manufacturing process of the buttercup juice. By maintaining the temperature, it was possible to prepare a buttercup juice preserved as it is without killing the active ingredient in the buttercup.
반면, <비교예 1 내지 2>와 같이 숙성 조건을 변경했을 때는 소화가 잘되지 않아 기호도가 다소 낮은 것으로 나타났다. 또한, <비교예 3 내지 4>과 같이 배합 조건을 달리한 경우에는 미나리 특유의 강한 향이 많이 느껴지거나 사과 및 레몬의 과일의 맛이 강하게 느껴졌으며, <비교예 5>에 의해 제조된 미나리 주스는 고온에서 멸균시켜 유효성분이 사멸하고 식감도 좋지 않았다. On the other hand, when the aging conditions were changed as in <Comparative Examples 1 to 2>, the digestibility was poor and the preference was rather low. In addition, when the mixing conditions were different as shown in <Comparative Examples 3 to 4>, a strong smell peculiar to buttercup was felt or the fruit taste of apple and lemon was strongly felt, and the buttercup juice prepared by <Comparative Example 5> was Sterilized at high temperature, the active ingredient was killed and the texture was not good.
<실험예 2> 소화흡수율 확인<Experimental Example 2> Check digestion absorption rate
상기 실시예 1 내지 3, 및 비교예 1 내지 5에서 각각 제조한 미나리 주스의 소화흡수 효능을 알아보기 위한 임상실험을 실시하였다.Clinical experiments were conducted to determine the digestive and absorption efficacy of buttercup juices prepared in Examples 1 to 3 and Comparative Examples 1 to 5, respectively.
유의차를 줄이기 위하여 유사한 성별 및 나이 구성을 위해, 각 실험군 당 30대 성인 남성 15명과 성인 여성 15명을 실험대상으로 하였다.To reduce the significant difference, 15 male and 30 adult females in their 30s were tested for similar sex and age composition.
각 실험군 당 해당 주스를 1주일 동안 하루에 250 ml 한 컵씩 섭취하였고, 실험 전 3일부터 종료 때까지 또 다른 과일이나 과일 주스를 섭취하지 못하도록 하여 실시하였다. 소화흡수 평가는 1주일 실험동안 변의 상태, 쾌변 및 위의 편안함 정도의 총 3가지를 10점 만점 기준으로 평가하였고, 변의 색깔은 육안으로 관찰하였다.The corresponding juice per each experimental group was consumed one cup of 250 ml per day for one week, and it was carried out by preventing another fruit or fruit juice from being consumed from the 3rd day before the experiment. For digestion and absorption evaluation, a total of three items, stool condition, comfort, and stomach comfort level, were evaluated on a 10-point scale during the one-week experiment, and the color of the stool was visually observed.
채점 기준은 다음과 같다.The scoring criteria are as follows.
아주 좋다 : 10점Very good: 10 points
좋다 : 8점Good: 8 points
보통이다 : 6점Normal: 6 points
나쁘다 : 4점Bad: 4 points
아주 나쁘다 : 2점Very bad: 2 points
그 결과를 표 2에 나타내었다.Table 2 shows the results.
구분division 변의 상태Stool 변의 색Side color 쾌변Pleasure 위의 편안함Comfort above
실시예 1Example 1 8.48.4 일반 변 색과 큰 차이가 없음No significant difference from normal discoloration 8.48.4 8.78.7
실시예 2Example 2 8.28.2 일반 변 색과 큰 차이가 없음No significant difference from normal discoloration 8.38.3 8.38.3
실시예 3Example 3 8.18.1 일반 변 색과 큰 차이가 없음No significant difference from normal discoloration 8.28.2 8.48.4
비교예 1Comparative Example 1 5.15.1 짙은 초록색 변Dark green stools 5.05.0 4.94.9
비교예 2Comparative Example 2 5.45.4 연한 초록색 변Light green stools 5.25.2 5.15.1
비교예3Comparative Example 3 6.36.3 연한 초록색 변Light green stools 6.06.0 5.95.9
비교예 4Comparative Example 4 6.56.5 연한 초록색 변Light green stools 5.85.8 5.75.7
비교예 5Comparative Example 5 6.66.6 연한 초록색 변Light green stools 5.75.7 5.65.6
임상실험 결과, <실시예 1 내지 3>에 의해 제조된 미나리 주스를 음용한 경우, 대부분의 피험자들 변의 상태, 쾌변 정도, 위의 편안함이 모두 8.0의 좋음 이상을 나타냈으며, 변의 색 또한 일반 변 색과 큰 차이가 없는 것으로 나타났다.As a result of clinical trials, when the buttercup juice prepared by <Examples 1 to 3> was ingested, most of the subject's stool state, degree of comfort, and stomach comfort all showed good or better of 8.0, and the color of the stool was also a general stool. There was no significant difference from the color.
반면, <비교예 1 내지 2>에 의해 제조된 미나리 주스를 음용한 경우, 피험자들이 소화불량을 호소하였으며 변의 상태와 쾌변정도, 위의 편안함 모두 좋지 않은 것으로 나타났고, 특히 저온 숙성 과정을 거치지 않은 <비교예 1>의 미나리 주스를 음용한 피험자들의 경우, 변의 색이 짙은 초록색을 많이 띄고 있는 것으로 보아, 장내에 흡수되지 못하고 변을 통해 다량 배출된 것을 알 수 있었다.On the other hand, when drinking the buttercup juice prepared by <Comparative Examples 1 to 2>, the subjects complained of indigestion, and the condition of the stool, the degree of comfort, and the comfort of the stomach were found to be poor, especially in the low temperature aging process. In the case of the subjects who consumed the buttercup juice of <Comparative Example 1>, it was found that the color of the stool had a lot of dark green color, and it was found that it was not absorbed in the intestine and was discharged in large amounts through the stool.
또한, 미나리와 사과, 레몬 등의 배합비율을 달리한 <비교예 3 내지 4>의 미나리 주스를 음용한 경우, 맛과 식감이 좋지 않아 변의 상태, 쾌변 정도 및 위의 편안함이 보통 수준을 나타내고 있으며, 멸균조건을 달리한 <비교예 5> 의 미나리 주스를 음용한 경우에도 변의 상태와 쾌변 정도, 위의 편안함이 모두 보통 수준인 것으로 나타났으며 <비교예 3 내지 5> 피험자들의 변의 색깔은 연한 초록색을 띄는 것을 확인하였다.In addition, when drinking the buttercup juices of <Comparative Examples 3 to 4> with different mixing ratios of buttercups, apples, and lemons, the taste and texture are not good, and the condition of the stool, the degree of comfort, and the comfort of the stomach indicate normal levels. , Even in the case of drinking the buttercup juice of <Comparative Example 5> with different sterilization conditions, the stool condition, the degree of comfort, and the above comfort were all at the normal level. <Comparative Examples 3 to 5> The color of the stools of the subjects was light. It was confirmed to have a green color.

Claims (6)

  1. 미나리를 세척한 후 착즙하는 제1 단계;After washing the buttercup, the first step of juice;
    착즙액을 0 내지 15℃에서 4일 내지 6일간 저온 숙성하는 제2 단계; 및A second step of aging the juice solution at 0 to 15° C. for 4 to 6 days at low temperature; And
    저온 숙성 미나리즙에 사과즙, 레몬즙을 혼합하는 제3 단계;를 포함하는 미나리주스의 제조방법.A third step of mixing apple juice and lemon juice in a low-temperature aged buttercup juice.
  2. 청구항 1에 있어서,The method according to claim 1,
    상기 제3 단계에서 미나리즙, 사과즙 및 레몬즙은 10 내지 30 중량부, 68 내지 88 중량부, 1 내지 3 중량부로 혼합하는 것을 특징으로 하는 미나리주스의 제조방법.In the third step, the buttercup juice, apple juice and lemon juice are mixed with 10 to 30 parts by weight, 68 to 88 parts by weight, and 1 to 3 parts by weight.
  3. 청구항 1에 있어서, The method according to claim 1,
    상기 제3 단계의 사과즙 및 레몬즙은 사과 또는 레몬을 세척한 후 착즙한 것을 포함하는 미나리주스의 제조방법.The third step of the apple juice and lemon juice is a method of producing a buttercup juice, including the apple or lemon after washing.
  4. 청구항 1에 있어서,The method according to claim 1,
    상기 제3 단계를 통해 제조된 혼합액에 멸균 및 포장하는 단계를 더 포함하는 것을 특징으로 하는 미나리주스의 제조방법.Method of manufacturing a buttercup, characterized in that it further comprises the step of sterilizing and packaging in the mixed solution prepared through the third step.
  5. 청구항 4에 있어서,The method according to claim 4,
    상기 멸균은 펄스전기장치(Pulsed Electric Field, PEF)에서 비가열 저온 멸균하는 것을 특징으로 하는 미나리주스의 제조방법.The sterilization method of manufacturing a buttercup, characterized in that the non-heating low-temperature sterilization in a pulsed electric device (Pulsed Electric Field, PEF).
  6. 미나리 착즙 저온 숙성액, 사과즙 및 레몬즙을 포함하는 장내 소화흡수율이 향상된 미나리주스 음료.A buttercup juice beverage with improved digestive absorption rate in the intestine, including a low temperature aging liquid of apple juice, apple juice, and lemon juice.
PCT/KR2018/015841 2018-11-30 2018-12-13 Oenanthe javanica juice having increased intestinal uptake and preparation method therefor WO2020111367A1 (en)

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* Cited by examiner, † Cited by third party
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