WO2019245078A1

WO2019245078A1 - Stem cell proliferation device

Info

Publication number: WO2019245078A1
Application number: PCT/KR2018/006962
Authority: WO
Inventors: 김성욱
Original assignee: 주식회사 디오스템스
Priority date: 2018-06-20
Filing date: 2018-06-20
Publication date: 2019-12-26

Abstract

The present invention relates to a stem cell proliferation device. A stem cell proliferation device according to an embodiment of the present invention comprises: a proliferation chamber shielded from the outside; a proliferation unit positioned in the proliferation chamber and configured to proliferate a stem cell while the stem cell and a proliferation medium are circulated; a supply unit for supplying the stem cell or the proliferation medium to the proliferation unit; an extraction unit for extracting the stem cell or the proliferation medium from the proliferation unit; a first gas supply unit for supplying a first gas into the proliferation unit; a second gas supply unit for supplying a second gas into the proliferation unit; and a pressure adjustment unit for adjusting the inner pressure of the proliferation unit, which is increased by the first gas and the second gas supplied into the proliferation unit.

Description

[Revision 11.09.2018 by Rule 26] Stem Cell Proliferation

The present invention relates to a stem cell proliferator.

Stem cells are capable of differentiating into various body tissue cells such as bone, nerve, muscle, cartilage, etc., depending on the method of obtaining, embryonated embryonic stem cells, somatic cell cloned embryonic stem cells, inverted induced stem cells, and adults Stem cells and the like.

In the case of the adult stem cells (Adult Stem Cell), which is extracted from the fat, bone marrow, umbilical cord blood, etc. of the adult, in the case of autologous transplantation is unlikely to generate an immune rejection reaction, there is an advantage that the stable transplantation. In the case of such adult stem cells, it is extracted from the fat of the adult and then used to proliferate it.

In general, for the proliferation of stem cells, the supply of carbon dioxide for the control of oxygen and pH for the respiration of the cell is required. Republic of Korea Patent Publication No. 2012-0063154 (name: stem cell culture device, hereinafter referred to as 'prior art document 1') and Republic of Korea Patent No. 1507621 (name: continuous with a moving container for producing culture bacteria in a continuous manner The culture apparatus, hereinafter referred to as "prior patent document 2", discloses a growth apparatus according to the prior art.

Referring to the prior art document 1, conventionally, oxygen and carbon dioxide are supplied into the culture chamber 100 by the gas adjusting means 200. Then, referring to the prior patent document 2, conventionally, live cells grow on the surface of the pipe 1 by the growth medium contained in the flexible pipe 1, and the clamps / gates 3 (4) ( In accordance with the opening and closing by 5), the pipe 1 is divided into an upstream region of the unused culture medium 7, a downstream region of the used culture medium 15, and a growth chamber region 10 between the upstream region and the downstream region. And conventionally, continuous growth of live cells is achieved while the pipe 1 is continuously moved, ie, continuously moved to the upstream region-the growth chamber region 10-downstream region.

However, according to the multiplier according to the prior art, the following problems occur.

First, simply supply oxygen and carbon dioxide to the interior of the culture chamber 100 according to the ratio of oxygen and carbon dioxide inside the culture chamber 100, and control the internal pressure of the culture chamber 100 according to the supply or discharge of oxygen and carbon dioxide. The technique to do is not disclosed. Therefore, in the related art, the ratio of oxygen and carbon dioxide in the culture chamber 100 may be maintained, but there is a concern that the internal pressure of the culture chamber 100 may be excessively increased in the process of maintaining it.

Moreover, conventionally, the area | region of the piping 1 is opened and closed by clamp / gate 3, 4, 5, and each area | region is divided. Therefore, in the related art, since the pipe 1 has to be molded of a flexible material in order to divide the area by the clamps / gates 3, 4, 5, the clamps / gates 3, 4, 5 The pipe 1 may be damaged during the opening and closing process.

In addition, conventionally, since the tube 1 must be sequentially partitioned into an upstream region-growth region 10-downstream region for cell proliferation, a predetermined length of space must be secured. Therefore, in the related art, there is a disadvantage that the space for installing the cell multiplier, that is, the cell proliferation is unnecessarily consumed.

The present invention is to solve the problems caused by the prior art as described above, an object of the present invention, to provide a stem cell multiplier configured to enable the control of the internal pressure according to the supply of oxygen and carbon dioxide.

Another object of the present invention is to provide a stem cell proliferator configured to ensure durability.

Still another object of the present invention is to provide a stem cell proliferator which is more compactly configured.

One aspect of the stem cell multiplier according to an embodiment of the present invention for achieving the above object is a growth chamber shielded from the outside; A proliferation unit positioned inside the proliferation chamber and configured to proliferate stem cells while circulating stem cells and proliferation medium; A supply unit for supplying a stem cell or a growth medium to the growth unit; An extraction unit for extracting stem cells or growth medium from the growth unit; A first gas supply unit supplying a first gas into the propagation unit; A second gas supply unit supplying a second gas into the propagation unit; And a pressure regulating unit for adjusting an internal pressure of the propagation unit increased by the first and second gases supplied into the propagation unit. It includes.

In one aspect of an embodiment of the present invention, the proliferation unit includes a proliferation tube through which stem cells are proliferated by receiving stem cells and proliferation medium; And a circulation pump for pumping the inside of the growth tube so that the stem cells and the growth medium circulate. It includes.

In one aspect of an embodiment of the present invention, the proliferation unit is for circulating stem cells and proliferation medium or supplying stem cells or proliferation medium to the proliferation tract or extracting stem cells or proliferation medium from the proliferation tract And an on / off valve for selectively opening and closing the growth tube.

In one aspect of an embodiment of the present invention, the growth tube, the spiral tube portion formed in a spiral shape; And an extension pipe part connected to both ends of the spiral pipe part to form a closed loop. It includes.

In one aspect of an embodiment of the present invention, the first gas supply unit comprises: a first gas supply fan that sucks outside air of the propagation chamber including a first gas; A first gas supply pipe through which air sucked by the first gas supply fan flows and is delivered to the propagation unit; And a first gas supply valve for selectively opening and closing the first gas supply pipe to deliver air to the propagation unit. It includes.

In an aspect of an embodiment of the present invention, the second gas supply unit includes: a second gas storage tank in which a second gas having a relatively high pressure is stored relative to an internal pressure of the propagation unit; A second gas supply pipe through which the second gas stored in the second gas storage tank flows and is delivered to the propagation unit; And a second gas supply valve for selectively opening and closing the second gas supply pipe to deliver a second gas to the propagation unit. It includes.

In one aspect of an embodiment of the present invention, the pressure regulating unit is connected to the growth unit, the pressure to flow the gas comprising a first gas and a second gas circulating the growth unit together with stem cells and growth medium Regulating chamber; A pressure sensor for sensing an internal pressure of the pressure regulation chamber; And a pressure regulating valve for discharging the gas flowed into the pressure regulating chamber to the outside according to the internal pressure of the pressure regulating chamber sensed by the pressure sensor. It includes.

In an aspect of an embodiment of the present invention, the pressure regulating unit further includes a diesel passage through which gas discharged to the outside of the pressure regulating chamber passes.

In one aspect of an embodiment of the present invention, the supply unit, the supply pipe is connected to the growth unit, the stem cell or the growth medium to be supplied to the growth unit therein; And a supply valve for selectively opening and closing the supply pipe to supply a stem cell or a growth medium to the growth unit. It includes.

In one aspect of the embodiment of the present invention, the supply unit further comprises a supply port to which the supply means for supplying the stem cells or the growth medium to the growth unit through the supply pipe.

In one aspect of an embodiment of the present invention, the extraction unit is connected to the propagation unit, the inside of the extraction tube flows stem cells or proliferation medium is extracted from the growth unit; And an extraction valve for selectively opening and closing the extraction tube to extract stem cells or growth medium from the growth unit. It includes.

In one aspect of an embodiment of the present invention, the extraction unit further comprises an extraction port connected to the extraction means for extracting stem cells or growth medium from the growth unit through the extraction tube.

One aspect of an embodiment of the present invention further includes a temperature maintaining unit for maintaining the internal temperature of the propagation chamber at a predetermined reference temperature.

In one aspect of an embodiment of the present invention, the temperature maintaining unit comprises: a circulation fan circulating internal air in the propagation chamber; And a cooling / heating unit for cooling or heating the internal air circulating in the growth chamber by the circulation fan such that the internal temperature of the growth chamber maintains a reference temperature. It includes.

According to the stem cell multiplier according to an embodiment of the present invention can be expected the following effects.

First, in the embodiment of the present invention, when the internal pressure of the propagation unit is adjusted by the gas supplied to the propagation unit in which the stem cells are propagated, the gas inside the propagation unit is discharged by the pressure regulating unit, thereby the internal of the propagation unit. The pressure is regulated. Therefore, according to the embodiment of the present invention,

In the embodiment of the present invention, stem cells are proliferated by circulation of stem cells and proliferation medium in the proliferation unit where stem cells are proliferated. Therefore, according to the embodiment of the present invention, it is possible to use a propagation tube having a predetermined rigidity, thereby ensuring the durability of the system.

In addition, in the embodiment of the present invention, by forming a portion of the growth tube in a spiral shape, the overall size and volume of the stem cell proliferative phase can be reduced. Therefore, according to the embodiment of the present invention, by adjusting only the temperature of the relatively small size and volume of the space, it is possible to more easily control the environment for the proliferation of stem cells.

1 is a plan view showing a stem cell proliferator according to an embodiment of the present invention.

2 to 6 is a state diagram showing the operation of the stem cell multiplier according to an embodiment of the present invention.

Figure 7 is a cross-sectional view showing a filter assembly constituting a stem cell multiplier according to an embodiment of the present invention.

Hereinafter, with reference to the accompanying drawings, the stem cell proliferator according to an embodiment of the present invention will be described in more detail.

Referring to FIG. 1, the stem cell growth apparatus 1 according to the present embodiment includes a growth chamber 100, a growth unit 200, a supply unit 300, an extraction unit 400, and a first gas supply unit 500. ), A second gas supply unit 600 and a pressure regulating unit 700. In the present embodiment, the stem cell multiplier 1 further includes a temperature maintaining unit 800.

More specifically, the proliferation chamber 100 defines a space in which the proliferation of stem cells can be substantially performed. The growth chamber 100 is shielded from the outside to maintain a predetermined condition for the proliferation of stem cells.

The proliferation unit 200 is located inside the proliferation chamber 100 and is where the stem cells proliferate while the stem cells and the proliferation medium circulate. In this embodiment, the propagation unit 200 includes a propagation pipe 210, a circulation pump 220, and an opening / closing valve 230. The growth tube 210 is where the stem cells are proliferated by receiving the stem cells and the growth medium. Substantially, stem cells implanted on the inner surface of the growth tube 210 will be propagated by the growth medium. The growth tube 210 includes a spiral tube portion 211 and an extension tube portion 212. The spiral tube portion 211 is formed in a spiral shape, the extension tube portion 212, both ends of the spiral tube portion 211 is connected to both ends to form a closed loop (close-loop). The circulation pump 220 pumps the stem cells and the growth medium to circulate the inside of the growth tube 210. The opening / closing valve 230 is for circulating stem cells and growth medium or for supplying stem cells or growth medium to the growth tube 210 or for extracting stem cells or growth medium from the growth tube 210. The growth tube 210 is selectively opened and closed.

On the other hand, the supply unit 300 supplies the stem cells or growth medium to the growth unit 200. In this embodiment, the supply unit 300 includes a supply pipe 310, a supply valve 320, and a supply port 330. The supply pipe 310 is connected to the growth unit 200, substantially the growth tube 210, the stem cells or the growth medium supplied to the growth tube 210 is flowed therein. The supply valve 320 selectively opens and closes the supply pipe 310 to supply the stem cells or the growth medium to the growth unit 200, substantially the growth tube 210. In addition, the supply port 330 is connected to the supply means (not shown) for supplying a stem cell or a growth medium to the growth unit 200 through the supply pipe 310.

Next, the extraction unit 400 extracts a stem cell or a growth medium from the growth unit 200. Substantially, the extraction unit 400 is for extracting the stem cells proliferated in the proliferation unit 200 and the proliferation medium used for proliferation of the stem cells to the outside. In the present embodiment, the extraction unit 400 includes an extraction tube 410, the extraction valve 420 and the extraction port 430. The extraction tube 410 is connected to the growth unit 200, substantially, the growth tube 210, the stem cells or the growth medium extracted from the growth unit 200 therein flows. The extraction valve 420 selectively opens and closes the extraction tube 410 in order to extract stem cells or growth medium from the growth unit 200 and substantially the growth tube 210. The extraction port 430 is where the extraction means (not shown) for extracting stem cells or growth medium from the growth unit 200 through the extraction tube 410 is connected.

The first and second

gas supply units

500 and 600 supply the first and second gases to the interior of the propagation unit 200, respectively. Here, the "first and second gas" is a gas used for culturing stem cells or necessary for controlling the culture conditions. Hereinafter, the description will be given on the assumption that oxygen and carbon dioxide are the first and second gases.

In more detail, the first gas supply unit 500 includes a first gas supply fan 510, a first gas supply pipe 520, and a first gas supply valve 530. The first gas supply unit 500 sucks the outside air of the growth chamber 100 containing oxygen. Although not shown, for this purpose, one side of the growth chamber 100 may be provided with a configuration for sucking outside air into the growth chamber 100 by the first gas supply fan 510. In the first gas supply pipe 520, air sucked by the first gas supply fan 510 flows therein and is delivered to the propagation unit 200. The first gas supply valve 530 selectively opens and closes the first gas supply pipe 520 to deliver air containing oxygen to the propagation unit 200. Of course, the second gas supply unit 600 may supply the stored or generated first gas, that is, oxygen, to the propagation unit 200 instead of the outside air of the propagation chamber 100. In this case, the first gas supply fan 510 may discharge oxygen stored in an oxygen storage tank (not shown) or oxygen generated from an oxygen generator (not shown) to the propagation unit 200.

In addition, the second gas supply unit 600 includes a second gas storage tank 610, a second gas supply pipe 620, and a second gas supply valve 630. The second gas storage tank 610 stores carbon dioxide having a relatively high pressure as compared with an internal pressure of the propagation unit 200. In the second gas supply pipe 620, carbon dioxide stored in the second gas storage tank 610 flows therein and is delivered to the propagation unit 200. The second gas supply valve 630 selectively opens and closes the second gas supply pipe 620 to deliver carbon dioxide to the propagation unit 200. As another example, the second gas supply unit 600 may supply carbon dioxide generated by a carbon dioxide generator (not shown) to the propagation unit 200, not carbon dioxide stored in the second gas storage tank 610. .

Next, the pressure regulating unit 700 adjusts the internal pressure of the propagation unit 200 increased by oxygen and carbon dioxide supplied into the propagation unit 200. In other words, since the growth unit 200, that is, the growth pipe 210 forms a closed loop shielded from the outside, in this embodiment, oxygen and carbon dioxide in the interior of the growth pipe 210 It is to prevent the problem caused by the internal pressure of the growth pipe 210 is increased by the supply by the pressure control by the pressure control unit 700.

In the present embodiment, the pressure regulating unit 700 includes a pressure regulating chamber 710, a pressure sensor (not shown), and a pressure regulating valve 720. The pressure control chamber 710 includes oxygen and carbon dioxide connected to the growth unit 200, substantially the growth tube 210, and circulating the growth unit 200 with stem cells and growth medium. Gas to flow. For example, one side of the extension pipe portion 212 may be connected to the pressure control chamber 710. The pressure sensor detects an internal pressure of the pressure regulation chamber 710. The pressure regulating valve 720 discharges the gas flowed into the pressure regulating chamber 710 to the outside according to the internal pressure of the pressure regulating chamber 710 sensed by the pressure sensor. For example, when the internal pressure of the pressure regulating chamber 710 reaches a preset reference pressure, the pressure regulating valve 720 supplies gas flowed into the pressure regulating chamber 710 to the pressure regulating chamber. It may be discharged to the outside of the 710, preferably to the outside of the growth chamber 100. In this case, the reference pressure for determining the opening and closing operation of the pressure regulating valve 720 may be set in consideration of factors such as the shape, size, length and material of the growth tube 210.

In addition, in the present embodiment, the pressure control unit 700 further includes a diesel chamber 730. The gas passage chamber 730 is a place where gas discharged to the outside of the pressure control chamber 710 passes. Substantially, when the pressure regulating valve 720 is opened, gas flowed into the pressure regulating chamber 710 is discharged through the gas passage chamber 730, and stem cells and the growth medium are passed through the gas passage chamber. It can be captured inside 730.

On the other hand, the temperature maintaining unit 800 serves to maintain the internal temperature of the growth chamber 100 to a predetermined reference temperature. In the present embodiment, the temperature maintaining unit 800 includes a circulation fan 810 and a cooling / heating unit 820. The circulation fan 810 circulates internal air of the growth chamber 100. The cooling / heating unit 820 cools or cools the internal air circulating inside the growth chamber 100 by the circulation fan 810 such that the internal temperature of the growth chamber 100 maintains a reference temperature. It serves to heat. For example, the cooling / heating unit 820 may be provided on an edge surface of the growth chamber 100, and the circulation fan 810 may be in contact with the cooling / heating unit 820. Inner air of the chamber 100 may circulate inside the growth chamber 100.

Hereinafter will be described in more detail with reference to the accompanying drawings, the action of the stem cell proliferator according to an embodiment of the present invention.

First, referring to FIG. 2, in order to supply the stem cells or the growth medium to the growth unit 200, that is, the growth tube 210 by the supply unit 300, the supply valve 320 is opened and the opening / closing valve ( 230 and extraction valve 420 are shielded. Therefore, the stem cells or the growth medium supplied from the supply means (not shown) connected to the supply port 330 is not discharged to the outside through the extraction unit 400, it is present inside the growth tube 210.

Next, referring to FIG. 3, when the supply of the stem cells and the growth medium to the growth unit 200 is completed, the open / close valve 230 is opened, and the supply valve 320 and the extraction valve 420 are closed. Is shielded. In this state, the stem cells and the growth medium supplied to the growth tube 210 by the circulation pump 220 is transported to circulate the growth tube 210, the stem cells are proliferated.

4, when the proliferation of stem cells in the propagation unit 200 is completed, the extraction valve 420 is opened, and the open / close valve 230 and the supply valve 320 are shielded. Therefore, by the extraction means (not shown) connected to the extraction port 430, it is possible to extract the stem cells and the growth medium used for the proliferation of the stem cells proliferated from the growth unit 200.

Meanwhile, referring to FIG. 5, the first and second

gas supply units

500 and 600 may include first and second gases required for proliferation of stem cells in the growth unit 200, for example, Feed oxygen and carbon dioxide. That is, in the state in which the first gas supply valve 530 is opened, external air, substantially oxygen, of the growth chamber 100 is sucked by the first gas supply fan 510 and the first gas supply pipe is sucked. It is supplied to the propagation unit 200 through 520. In addition, while the second gas supply valve 630 is open, the carbon dioxide stored in the second gas storage tank 610 is supplied to the propagation unit 200 through the second gas supply pipe 620.

However, when oxygen and carbon dioxide are supplied to the propagation unit 200 by the first and second

gas supply units

500 and 600, the pressure of the propagation unit 200 will be increased. Referring to FIG. 6, in the present embodiment, when the internal pressure of the propagation unit 200 reaches a preset reference pressure, the gas flowing through the propagation unit 200 by the pressure regulating unit 700 propagates the propagation unit. By discharging to the outside of the unit 200, the internal pressure of the propagation unit 200 is adjusted.

More specifically, when the pressure inside the propagation unit 200 sensed by the pressure sensor, substantially the internal pressure of the pressure control chamber 710 reaches a reference pressure, the pressure control valve 720 is opened. Therefore, the gas including the oxygen and carbon dioxide flowing from the propagation unit 200 to the pressure control chamber 710 is discharged to the outside of the pressure control chamber 710, so that the pressure control chamber 710, substantially the The internal pressure of the propagation unit 200 may be maintained below the reference pressure. In particular, in the present embodiment, by the gas discharged to the outside of the pressure control chamber 710 via the gas passage chamber 730, it can be discharged together in the process of the gas discharged to the outside of the pressure control chamber 710. Stem cells or proliferation medium may be collected in the gas passage chamber 730.

The scope of the present invention is not limited to the embodiments described above, but is defined by the claims, and various modifications and adaptations within the scope of the claims are made by those skilled in the art. It is self evident.

Hereinafter, with reference to the accompanying drawings, a filter assembly constituting a stem cell multiplier according to an embodiment of the present invention will be described in more detail.

7 is a cross-sectional view showing a filter assembly constituting a stem cell proliferator according to an embodiment of the present invention.

Referring to FIG. 7, the filter assembly 900 according to the present embodiment may filter foreign substances from stem cells supplied to the stem cell multiplier 1 or stem cells cultured and extracted in the stem cell multiplier 1. Do it. In the present embodiment, the filter assembly 900 includes a first filter unit 910, a second filter unit 920, and a container 930.

In more detail, the first filter unit 910 filters foreign matter from stem cells. The first filter unit 910 includes a first filter frame 911 and a second filter member 925. The first filter frame 911 may be formed in a cylindrical shape in which an upper surface and a bottom surface are opened, respectively, and at least one opening may be formed in an edge surface thereof. In addition, the upper edge of the first filter frame 911 is provided with a first stopping bracket 912 supported on the upper end of the second filter frame 921 to be described later, the first filter frame 911 On one side, a first gripping rib 913 for gripping an operator may be provided. Substantially, the first stopping bracket 912 and the first gripping rib 913 may include a second filter frame 921 with the first filter frame 911 seated on the second filter frame 921. Outside of). The first filter member 915 is fixed to the first filter frame 911, and a plurality of first filter holes are formed with a predetermined first diameter. The first filter member 915 may be fixed to the bottom of the first filter frame 911 and the opening.

The second filter unit 920 filters the foreign matter from the stem cells passing through the first filter unit 910. The second filter unit 920 includes a second filter frame 921 and a second filter member 925. The second filter frame 921 may be formed in a cylindrical shape in which an upper surface and a bottom surface are opened, and at least one opening may be formed in an edge surface thereof. In addition, a second stopping bracket 922 supported at the upper end of the container 930 is provided at an upper edge of the second filter frame 921, and at one side of the second filter frame 921, an operator is provided. A second gripping rib 923 may be provided for gripping. Substantially, the second stopping bracket 922 and the second gripping rib 923 may be disposed outside the container 930 in a state in which the second filter frame 921 is seated in the container 930. Is located. The second filter member 925 is fixed to the second filter frame 921, and a plurality of second filter holes are formed with a predetermined second diameter. The second filter member 925 may be fixed to the bottom surface of the second filter frame 921 and the opening.

The container 930 is disposed on the stem cell multiplier 1, substantially, the supply port 330 or the extraction port 430 in a state where the first and

second filter units

910 and 920 are installed. do. Substantially, the container 930 delivers the stem cells in which foreign matter is filtered by the first and

second filter units

910 and 920 to the stem cell multiplier 1 or the first and second filter units. Stem cells filtered by the (910) 920 will serve to extract from the stem cell multiplier (1). To this end, the container 930 may have a cylindrical shape having an upper surface and a lower surface open, and a third gripping rib 933 may be provided at an upper end of the container 930.

In the present embodiment, the diameter of the second filter hole is set to a value equal to or less than the diameter of the first filter hole. Preferably, the diameter of the first filter hole may be set to 100 μm or less, and the diameter of the second filter hole may be set to 50 to 100 μm or less. Therefore, foreign matters may be sequentially filtered by the first filter unit 910 and the second filter unit 920.

In addition, in the present embodiment, the first and

second filter units

910 and 920 are sequentially seated in the container 930. To this end, in a state in which the first filter frame 911 is seated inside the second filter frame 921, the second filter frame 921 is seated inside the container 930.

Therefore, in the present embodiment, foreign matters, for example, fat, etc., may be removed from the stem cells supplied to the growth unit 200 or the stem cells extracted from the growth unit 200 by the filter assembly 900. Can be. In addition, in the present embodiment, sequential filtration of foreign matter by the second filter unit 920 that filters foreign matter having a relatively smaller size than the first filter unit 910 and the first filter unit 910. By this, it is possible to filter foreign matter from the stem cells more efficiently. In the present embodiment, since the first and

second filter units

910 and 920 may be sequentially removed from the container 930, easy use and cleaning of the filter assembly 900 may be expected. .

Furthermore, in the present embodiment, the first and second stopping

brackets

912 and 922 are formed in different sizes, and the first to

third grip ribs

913, 923 and 933 are formed in different sizes. do. For example, the first stopping bracket 912 is formed to be relatively larger than the second stopping bracket 922, and the first gripping rib 913 is larger than the second gripping rib 923. The second grip rib 923 may be formed relatively large, and the second grip rib 923 may be formed relatively larger than the third grip rib 933. Accordingly, the first and

second filter units

910 and 920 are seated in the container 930, that is, the first filter frame 911 is seated inside the second filter frame 921. In the state where the second filter frame 921 is seated inside the container 930, the first and second filter frames 911 and 921 are removed from the container 930 and the second filter. Removal of the first filter frame 911 from the frame 921 may be easier.

Claims

A propagation chamber 100 shielded from the outside;

A proliferation unit 200 positioned inside the proliferation chamber 100 and configured to proliferate stem cells while circulating stem cells and proliferation medium;

Supply unit 300 for supplying stem cells or growth medium to the growth unit 200;

An extraction unit 400 for extracting stem cells or growth media from the growth unit 200;

A first gas supply unit (500) for supplying a first gas into the propagation unit (200);

A second gas supply unit (600) for supplying a second gas into the propagation unit (200); And

A pressure regulating unit (700) for adjusting an internal pressure of the propagation unit (200) increased by the first and second gases supplied into the propagation unit (200); Stem cell proliferator comprising a.
The method of claim 1,

The propagation unit 200,

A proliferation tube 210 in which stem cells are proliferated by receiving stem cells and proliferation medium; And

A circulation pump 220 pumping the inside of the growth tube 210 to circulate stem cells and growth media; Stem cell proliferator comprising a.
The method of claim 2,

The proliferation unit 200 is configured to circulate stem cells and proliferation medium or to supply stem cells or proliferation medium to the proliferation tube 210 or to extract stem cells or proliferation medium from the proliferation tube 210. Stem cell multiplier further comprises an opening and closing valve 230 for selectively opening and closing the growth pipe (210).
The method of claim 2,

The growth tube 210,

A spiral tube portion 211 formed in a spiral shape; And

An extension pipe part 212 connected at both ends of the spiral pipe part 211 to form a closed loop; Stem cell proliferator comprising a.
The method of claim 1,

The first gas supply unit 500,

A first gas supply fan 510 for sucking outside air of the growth chamber 100 including a first gas;

A first gas supply pipe 520 through which air sucked by the first gas supply fan 510 flows and is delivered to the propagation unit 200; And

A first gas supply valve 530 that selectively opens and closes the first gas supply pipe 520 to deliver air to the propagation unit 200; Stem cell proliferator comprising a.
The method of claim 1,

The second gas supply unit 600,

A second gas storage tank 610 in which a second gas having a relatively high pressure is stored compared with an internal pressure of the propagation unit 200;

A second gas supply pipe 620 in which a second gas stored in the second gas storage tank 610 flows therein and is delivered to the propagation unit 200; And

A second gas supply valve 630 for selectively opening and closing the second gas supply pipe 620 to deliver a second gas to the propagation unit 200; Stem cell proliferator comprising a.
The method of claim 1,

The pressure control unit 700,

A pressure regulation chamber 710 connected to the propagation unit 200 and flowing a gas including first and second gases circulating with the stem cell and the propagation medium and circulating the propagation unit 200;

A pressure sensor detecting an internal pressure of the pressure regulation chamber 710; And

A pressure regulating valve 720 for discharging the gas flowed into the pressure regulating chamber 710 to the outside according to the internal pressure of the pressure regulating chamber 710 sensed by the pressure sensor; Stem cell proliferator comprising a.
The method of claim 7, wherein

The pressure control unit 700, the stem cell multiplier further comprises a gas passage chamber 730 through the gas discharged to the outside of the pressure control chamber (710).
The method of claim 1,

The supply unit 300,

A supply pipe 310 connected to the propagation unit 200 and in which a stem cell or a growth medium supplied to the propagation unit 200 flows; And

A supply valve 320 for selectively opening and closing the supply pipe 310 to supply a stem cell or a growth medium to the propagation unit 200; Stem cell proliferator comprising a.
The method of claim 9,

The supply unit 300, the stem cell multiplier further comprises a supply port 330 is connected to the supply means for supplying stem cells or growth medium to the growth unit 200 through the supply pipe 310.
The method of claim 1,

The extraction unit 400,

An extraction tube 410 connected to the propagation unit 200 and having a stem cell or a growth medium extracted from the propagation unit 200 therein; And

An extraction valve 420 for selectively opening and closing the extraction tube 410 to extract stem cells or growth medium from the growth unit 200; Stem cell proliferator comprising a.
The method of claim 11,

The extraction unit 400, the stem cell multiplier further comprises an extracting port 430 is connected to the extraction means for extracting stem cells or growth medium from the growth unit 200 through the extraction tube (410).
The method according to any one of claims 1 to 12,

Stem cell multiplier further comprises a temperature maintaining unit for maintaining the internal temperature of the growth chamber 100 to a predetermined reference temperature.
The method of claim 1,

The temperature maintaining unit 800,

A circulation fan 810 for circulating internal air in the propagation chamber 100; And

A cooling / heating unit 820 for cooling or heating internal air circulating in the growth chamber 100 by the circulation fan 810 such that the internal temperature of the growth chamber 100 maintains a reference temperature; Stem cell proliferator comprising a.