WO2019245070A1 - Device and method for proliferating stem cell, and system and method for continuously proliferating, extracting, and separating stem cell - Google Patents

Device and method for proliferating stem cell, and system and method for continuously proliferating, extracting, and separating stem cell Download PDF

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Publication number
WO2019245070A1
WO2019245070A1 PCT/KR2018/006931 KR2018006931W WO2019245070A1 WO 2019245070 A1 WO2019245070 A1 WO 2019245070A1 KR 2018006931 W KR2018006931 W KR 2018006931W WO 2019245070 A1 WO2019245070 A1 WO 2019245070A1
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Prior art keywords
stem cell
proliferation
stem cells
tube
growth
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PCT/KR2018/006931
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French (fr)
Korean (ko)
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김성욱
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주식회사 디오스템스
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Priority to PCT/KR2018/006931 priority Critical patent/WO2019245070A1/en
Publication of WO2019245070A1 publication Critical patent/WO2019245070A1/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M1/00—Apparatus for enzymology or microbiology
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M1/00—Apparatus for enzymology or microbiology
    • C12M1/12—Apparatus for enzymology or microbiology with sterilisation, filtration or dialysis means
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M1/00—Apparatus for enzymology or microbiology
    • C12M1/34—Measuring or testing with condition measuring or sensing means, e.g. colony counters
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M1/00—Apparatus for enzymology or microbiology
    • C12M1/42—Apparatus for the treatment of microorganisms or enzymes with electrical or wave energy, e.g. magnetism, sonic waves
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M3/00—Tissue, human, animal or plant cell, or virus culture apparatus
    • C12M3/04—Tissue, human, animal or plant cell, or virus culture apparatus with means providing thin layers

Definitions

  • the present invention relates to stem cell proliferative phase and proliferation method, stem cell continuous proliferation, extraction and separation system and method.
  • Stem cells are capable of differentiating into various body tissue cells such as bone, nerve, muscle, cartilage, etc., depending on the method of obtaining, embryonated embryonic stem cells, somatic cell cloned embryonic stem cells, inverted induced stem cells, and adults Stem cells and the like.
  • the adult stem cells (Adult Stem Cell), which is extracted from the fat, bone marrow, umbilical cord blood, etc. of the adult, in the case of autologous transplantation is unlikely to generate an immune rejection reaction, there is an advantage that the stable transplantation. In the case of such adult stem cells, it is extracted from the fat of the adult and then used to proliferate it.
  • Republic of Korea Patent No. 1507621 discloses a multiplier according to the prior art.
  • live cells grow on the surface of the pipe 1 by the growth medium contained in the flexible pipe 1, and the clamps / gates 3, 4, and 5 are applied.
  • the pipe 1 is divided into an upstream region of the unused culture medium 7, a downstream region of the culture medium 15 used, and a growth chamber region 10 between the upstream region and the downstream region in accordance with opening and closing by the opening and closing process.
  • continuous growth of live cells is achieved while the pipe 1 is continuously moved, ie, continuously moved to the upstream region-the growth chamber region 10-downstream region.
  • the present invention is to solve the problems caused by the prior art as described above, an object of the present invention, the stem cell proliferative phase and proliferation configured to enable the extraction and proliferation of continuous stem cells and separation for the proliferated cells It is to provide a method, a stem cell continuous proliferation, extraction and separation system and method.
  • Another object of the present invention is to provide a stem cell multiplier and proliferation method, a stem cell continuous proliferation, extraction and separation system and method configured to prevent the contamination of stem cells.
  • Still another object of the present invention is to provide a stem cell multiplier and proliferation method, a stem cell continuous proliferation, extraction and separation system and method, which are configured to more simply extract, proliferate, and separate continuous stem cells.
  • Still another object of the present invention is to provide a stem cell multiplier and a proliferation method, a stem cell continuous proliferation, an extraction and separation system and a method configured to ensure durability.
  • Still another object of the present invention is to provide a stem cell multiplier and a method of proliferation, a stem cell continuous proliferation, an extraction and separation system and a method which are more compactly configured.
  • One aspect of the stem cell multiplier for achieving the above object is a spiral tube portion formed in a spiral shape, the proliferation of stem cells and stem cells extracted from the outside extending from one end of the spiral portion
  • a proliferation tube including a first extension tube part receiving the proliferation medium for supplying the second extension tube part extending from the other end of the spiral part and supplying stem cells proliferated to the outside;
  • a medium supply unit supplying a growth medium to the growth tube;
  • a first opening / closing unit for selectively communicating / separating the first extension tube part with the first supply tube for supplying the stem cells extracted into the growth tube;
  • a second opening / closing unit for selectively communicating / separating the second extension tube part with a second supply tube for supplying stem cells proliferated in the growth tube;
  • a rotation drive unit for rotating the propagation tube.
  • the proliferation tube one end is connected to one side of the spiral tube portion or one side of the first extension tube portion adjacent to the first extension tube portion
  • the second extension Further comprising a circulation pipe portion having the other end connected to the other side of the spiral tube portion adjacent to the tube portion or one side of the second extension tube portion, the first and second extension tube portion and the first by the first and second opening and closing unit
  • a circulation pump for pumping the stem cells and the growth medium from one end of the spiral tube to the other end of the spiral tube so that the stem cells and the growth medium circulate the spiral tube in a state in which the first and second supply pipes are separated.
  • At least the spiral tube portion is disposed to be inclined at an angle of more than 0 ° and less than 45 ° with respect to the horizontal plane.
  • the growth tube is further disposed therein, further comprises a growth vessel that rotates with the growth tube by the rotation drive unit.
  • One aspect of the stem cell multiplier according to an embodiment of the present invention further comprises a temperature control unit for controlling the temperature for the proliferation of stem cells in the growth tube.
  • One aspect of the stem cell multiplier according to an embodiment of the present invention further comprises an air supply unit for supplying air to the growth tube.
  • the proliferation medium for proliferation of the extracted stem cells and stem cells is supplied to the growth tube Supplying the extracted stem cells and the growth medium; And stem cell proliferation step in which stem cells proliferate inside the growth tube; It includes.
  • the first extension tube portion and the first supply tube is communicated by the first opening and closing unit, the second The stem cells and the proliferation medium extracted through the first extension pipe part and the first supply pipe in a state where the second extension pipe part and the second supply pipe are separated by the opening / closing unit are supplied to the growth pipe.
  • the rotation drive unit, the first and second extension tube portion and the first by the first and second opening and closing unit in the stem cell proliferation step, the rotation drive unit, the first and second extension tube portion and the first by the first and second opening and closing unit
  • the propagation tube is rotated while the first and second supply tubes are separated.
  • a circulation pump pumps the stem cells and the proliferation medium to circulate the proliferation tube.
  • the temperature control unit in the stem cell proliferation step, the temperature control unit, the temperature for the proliferation of stem cells in the growth tube, the air supply unit, Supply air into the growth tube.
  • the stem cell extractor for extracting stem cells from the sample;
  • a stem cell multiplier according to any one of claims 1 to 6 for receiving and proliferating stem cells extracted from the stem cell extractor;
  • a centrifugal separator for receiving the stem cells proliferated from the stem cell multiplier to separate by component; It includes.
  • the stem cell extractor the first supply pipe for supplying the stem cell proliferation to the stem cell multiplier in communication with the first extension tube portion;
  • the centrifuge includes a second supply pipe that receives stem cells proliferated in communication with the second extension tube part from the stem cell multiplier.
  • stem cell extraction, proliferation and separation system in the state in which the first extension tube and the first supply tube in communication with the first opening and closing unit, extracted by the stem cell extractor And a first supply pump for pumping the stem cells and the growth medium supplied by the growth medium supply unit to the growth tube.
  • the stem cell multiplier in the state in which the second extension tube portion and the second supply tube is in communication by the second opening and closing unit, it is propagated by the stem cell multiplier It further comprises a second feed pump for pumping stem cells into the centrifuge.
  • the method for continuously proliferating, extracting and separating stem cells using the stem cell continuous proliferation, extraction and separation system of claim 12 Stem cells, the stem cell extraction step is extracted by the stem cell extractor; Extracting stem cells and a growth medium supplying a proliferation medium for proliferation of stem cells and stem cells extracted in the stem cell extraction step to the growth tube; A stem cell proliferation step of proliferating the stem cells supplied to the growth tube in the extracting stem cells and the growth medium supplying step in the growth tube; A proliferating stem cell supplying step of supplying the stem cells proliferated in the stem cell proliferation step to the centrifuge; And a stem cell separation step of separating the stem cells supplied in the proliferating stem cell supply step by component by the centrifuge. It includes.
  • the first extension tube and the first supply tube is communicated by the first opening and closing unit
  • Propagation medium from is supplied to the growth vessel.
  • the rotation drive unit, the first and second opening and closing unit by the first and second opening and closing unit in the stem cell proliferation step, the rotation drive unit, the first and second opening and closing unit by the first and second opening and closing unit
  • the propagation tube is rotated while the extension tube portion and the first and second supply tubes are separated.
  • the circulation pump, the first and second extension tube by the first and second opening and closing unit In the state where the first and second supply pipes are separated, stem cells and proliferation medium are pumped to circulate the proliferation pipes.
  • the stem cell extraction step may be performed regardless of the completion of the stem cell proliferation step. Is initiated.
  • the extracting stem cells and proliferation medium supplying step are independent of completion of the stem cell separation step. Is disclosed.
  • the stem cell continuous proliferation, extraction and separation system and method according to an embodiment of the present invention can be expected the following effects.
  • stem cells can be more quickly extracted, expanded, and separated by components.
  • the stem cells extracted from the stem cell extractor is directly supplied to the stem cell proliferator through the tube, the stem cells proliferated in the stem cell proliferator are directly supplied to the centrifuge through the tube. Therefore, according to the embodiment of the present invention, it is possible to prevent contamination during the movement of stem cells between the stem cell extractor, stem cell multiplier and centrifuge.
  • the extraction, proliferation and separation of the stem cells are continuously performed. Therefore, according to the embodiment of the present invention, it is possible to extract, proliferate, and separate components by successive stem cells in a simpler configuration.
  • stem cells are proliferated by the rotation of the proliferative tube through which the stem cells are proliferated and the circulation of the stem cells and the proliferation medium in the proliferating tube. Therefore, according to the embodiment of the present invention, it is possible to use a propagation tube having a predetermined rigidity, thereby ensuring the durability of the system.
  • the overall size and volume of the stem cell proliferative phase can be reduced. Therefore, according to the embodiment of the present invention, by adjusting only the temperature of the relatively small size and volume of the space, it is possible to more easily control the environment for the proliferation of stem cells.
  • FIG. 1 is a schematic view showing a stem cell continuous proliferation, extraction and separation system according to a first embodiment of the present invention.
  • Figure 2 is a flow chart showing the stem cell continuous proliferation, extraction and separation method according to a first embodiment of the present invention.
  • Figure 3 is a schematic diagram showing a stem cell continuous proliferation, extraction and separation system according to a second embodiment of the present invention.
  • FIG. 1 is a schematic view showing a stem cell continuous proliferation, extraction and separation system according to a first embodiment of the present invention.
  • the stem cell continuous proliferation, extraction and separation system 1 according to the present embodiment, stem cell extractor 100, stem cell multiplier 200, centrifuge 300 and the first and second Feed pumps 410 and 420.
  • the proliferation, extraction and separation of the stem cells are carried out continuously.
  • the stem cell extractor 100 extracts stem cells from a sample.
  • the stem cell extractor 100 may extract stem cells, that is, adult stem cells from adult fat, bone marrow, umbilical cord blood, and the like.
  • the stem cell extractor 100 may extract stem cells by various kinds of known extraction methods.
  • the stem cell extractor 100 includes a first supply pipe 110 for supplying the extracted stem cells to the stem cell multiplier 200.
  • the stem cell multiplier 200 receives and proliferates stem cells extracted from the stem cell extractor 100.
  • the growth tube 210 is a place where stem cells proliferate.
  • the growth tube 210 includes a spiral tube portion 211, the first and second extension tube portion 213, 215 and the circulation tube portion 217.
  • the spiral pipe portion 211 is formed in a spiral shape as a whole. Substantially the stem cells supplied into the growth tube 210 will be proliferated by the growth medium in a state implanted in the inner surface of the spiral tube portion 211.
  • the first extension tube portion 213 extends from one end of the spiral tube portion 211 positioned relatively upward to provide a growth medium for proliferation of stem cells and stem cells extracted from the stem cell extractor 100. To be supplied.
  • the second extension tube portion 215 extends from the other end of the spiral tube portion 211 positioned relatively downward to the stem cell multiplier 200 and substantially the stem cells proliferated in the growth tube 210. Supply to the centrifuge (300).
  • the circulation pipe 217, the stem cells and proliferation medium in particular, after the stem cells are implanted on the surface of the spiral pipe portion 211 mainly for the growth medium to circulate the spiral pipe portion 211 will be.
  • Substantially inside the circulation pipe 217 flows stem cells and proliferation medium, which are pushed to one end from the other end of the spiral pipe 211.
  • one end of the circulation pipe part 217 is connected to one side of the spiral pipe part 211 adjacent to the first extension pipe part 213 or to one side of the first extension pipe part 213, and the circulation pipe part 217 The other end of) is connected to the other end of the spiral tube portion 211 adjacent to the second extension tube portion 215 or to one side of the second extension tube portion 215.
  • stem cells may also be implanted into the inner surface of the circulation tube 217.
  • the spiral pipe portion 211 is disposed to be inclined at an angle of more than 0 ° and 45 ° or less with respect to the horizontal plane. This is to ensure efficient circulation of stem cells and stem cells and proliferation medium.
  • the spiral tube portion 211 is disposed at an angle of more than 45 ° with respect to the horizontal plane, the stem cells flow in the spiral tube portion 211 at an excessively high speed so that the stem cells flow. The idea of not being made enough.
  • the stem cells and the proliferation medium flow in the spiral tube portion 211 at a relatively excessively slow speed, thereby causing the stem Cells may be locally implanted on the inner surface of the spiral tube portion 211 or proliferation medium may not flow at a sufficient speed.
  • the stem cell and the proliferation medium are forcibly flowed from one end of the spiral tube portion 211 positioned relatively upward from the other end of the spiral tube portion 211 positioned relatively downward, and substantially the spiral tube portion 211
  • the flow of stem cells and proliferation medium in the inside is relatively slow due to its own weight. Therefore, in this embodiment, by limiting the inclination angle to the horizontal plane of the spiral tube portion 211 to more than 0 ° and less than 45 °, the implantation of stem cells and circulation of the growth medium is made to a sufficient level for the proliferation of stem cells To lose.
  • a coating layer may be provided on the inner surface of the growth tube 210 in order to induce the implantation of stem cells and to prevent toxic generation of stem cells in the proliferation process.
  • hyaluronic acid or hydroxy-apatite containing a nitroso-glutathione (GSNO), nitroso-cysteine (cysNO), or hydroxy-apatite (hydrogel) to prevent stem cell implantation and toxicity generation.
  • Hydrogel and parasitic polysaccharides SPS-CF: marine algalsulphated polysaccharides of Capsosiphon Fulvescens ) may be coated on the inner surface of the growth tube 210.
  • the growth vessel 220 may be formed in a hollow cylindrical shape of which the upper surface is approximately open. The growth vessel 220 is rotated in a state where the growth vessel 210 is disposed therein, thereby substantially rotating together with the growth vessel 210.
  • the growth medium supply unit 230 supplies a growth medium to the growth tube 210.
  • the growth medium supply unit 230 may be connected to, for example, the first supply pipe 110. Therefore, the growth medium supplied by the growth medium supply unit 230 may be supplied to the growth tube 210 together with the stem cells extracted from the stem cell extractor 100.
  • any one of GSH (Glutathione), GSNO (nitroso-glutathione), and CysNO (nitroso-cysteine) may be included in the growth medium supplied to the growth tube 210 to prevent the oxidation of stem cells.
  • the first opening and closing unit 241 selectively communicates / separates the first extension pipe part 213 and the first supply pipe 110.
  • the second opening and closing unit 242 selectively communicates / separates the second extension pipe part 215 and the second supply pipe 310 to be described later. Substantially, the supply of the extracted stem cells and the proliferation medium through the first extension tube 213 and the first supply tube 110 by the first opening and closing unit 241 is selectively made. And the second opening and closing unit 242 is selectively made of the supply of proliferated stem cells through the second extension tube 215 and the second supply tube 310.
  • the first opening and closing unit 241 includes a pair of first valves 241V for opening and closing the ends of the first extension pipe part 213 and the first supply pipe 110, respectively
  • the second The opening and closing unit 242 includes a pair of second valves 242V for opening and closing ends of the second extension pipe part 215 and the second supply pipe 310, respectively. Accordingly, the first extension pipe part 213 and the first supply pipe 110 communicate / separate according to the opening and closing of the first valve 241V, and the second extension pipe part (2) according to the opening and closing of the second valve 242V. 215 and the second supply pipe 310 will be in communication / separation.
  • the first extension tube portion 213 and the first supply tube 110 is in communication by the first opening and closing unit 241,
  • the second extension pipe part 215 and the second supply pipe 310 will be separated by the second opening and closing unit 242.
  • the first extension tube part 213 and the first supply tube 110 will be separated by the first opening / closing unit 241. .
  • the rotation drive unit 250 rotates the growth tube 210. Substantially in this embodiment, the rotation drive unit 250 rotates the growth vessel 220 in which the growth tube 210 is installed therein. Of course, in the state in which the growth vessel 220 is fixed, the rotation drive unit 250 may rotate the growth tube 210. However, the rotation of the propagation pipe 210 by the rotation driving unit 250 is performed by the first and second opening and closing units 241 and 242 to the first and second extension pipe parts 213 and 215. ) And the first and second supply pipes 110 and 310 in a separated state.
  • the circulation pump 260 pumps the stem cells and the proliferation medium to circulate the spiral tube portion 211 and the circulation tube portion 217. As described above, the circulation pump 260 substantially flows the stem cells and the growth medium from one end of the spiral tube portion 211 to one end.
  • the pumping of the stem cells and the proliferation medium by the circulation pump 260 is performed by the first and second opening and closing units 241 and 242 and the first and second extension pipe parts 213 and 215. In a state in which the first and second supply pipes 110 and 310 are separated, it will be made.
  • the temperature control unit 270 adjusts the temperature for the proliferation of stem cells in the growth tube 210.
  • the temperature control unit 270 may be installed at one side of the growth vessel 220 to adjust the internal temperature of the growth vessel 220 to maintain a predetermined temperature.
  • the air supply unit 280 supplies air to the growth tube 210.
  • the air supply unit 280 is connected to one side of the growth tube 210, for example, one side of the spiral tube portion 211, and supplies air to the interior of the growth tube 210.
  • the air supply unit 280 supplies air to the growth pipe 210 in which the concentration of carbon dioxide is increased relative to the atmosphere.
  • the air supplied to the growth pipe 210 by the air supply unit 280 may be supplied in a state in which foreign matters and the like are removed by the filter 281.
  • the centrifuge 300 receives the stem cells proliferated from the stem cell multiplier 200 and separates by component.
  • the centrifuge 300 various kinds of well-known centrifuges may be used.
  • the centrifuge 300 includes a second supply pipe 310.
  • the second supply pipe 310 is in communication with the second extension tube 215 receives the stem cells proliferated from the stem cell multiplier 200.
  • the first supply pump 410 pumps the stem cells extracted by the stem cell extractor 100 and the growth medium supplied by the growth medium supply unit 230 to the growth tube 210.
  • the second supply pump 420 pumps the stem cells proliferated by the stem cell multiplier 200 to the centrifuge 300. Substantially, the pumping of the stem cells and the proliferation medium extracted by the first supply pump 410 and the pumping of the stem cells proliferated by the second supply pump 420 may be performed by the first extension pipe part 213 and, respectively. In a state in which the first supply pipe 110 is in communication, or in a state in which the second extension pipe part 215 and the second supply pipe 310 are in communication, it will be made.
  • Figure 2 is a flow chart showing a method of continuous proliferation, extraction and separation of stem cells according to the first embodiment of the present invention.
  • stem cells are extracted from the sample by the stem cell extractor (100).
  • the extraction of stem cells by the stem cell extractor 100 may use a well-known technique, and thus a detailed description thereof will be omitted.
  • the proliferation medium for proliferation of the stem cells and stem cells extracted in the stem cell extraction step (S100) is a stem cell multiplier 200, substantially proliferating Supplied to the tube 210. More specifically, the stem cells extracted from the stem cell extractor 100 and the growth medium supplied by the growth medium supply unit 230 may be pumped to the growth tube 210 by the first supply pump 410. will be. Supply of the extracted stem cells and proliferation medium, the first extension tube portion 213 and the first supply tube 110 is communicated by the first opening and closing unit 241, the second extension tube portion 215 and the second supply tube ( The 310 is separated by the second opening and closing unit 242, it will be made through the first extension pipe portion 213 and the first supply pipe 110.
  • the stem cells supplied to the growth tube 210 in the extracting stem cells and growth medium supply step (S200) is proliferated inside the growth tube 210.
  • the stem cell proliferation step (S300) rotation of the growth tube 210 and circulation of the stem cells and the growth medium in the growth tube 210, substantially the spiral tube portion 211 is made.
  • the first and second extension tube portion 213, 215 and the first and second supply tube 110, 310 is the first and second In the state separated by the opening / closing unit 241 or 242, the rotation driving unit 250 rotates the growth tube 210.
  • the first and second extension pipe parts 213 and 215 and the first and second supply pipes 110 and 310 are connected to the first and second opening and closing units 241.
  • the circulation pump 260 pumps the stem cells and the growth medium to circulate the growth pipe 210.
  • the stem cell growth step (S300), the temperature control by the temperature control unit 270 and the air supply by the air supply unit 280 is also made. That is, in the stem cell growth step (S300), the temperature control unit 270, the temperature for the growth of stem cells in the growth tube 210, the air supply unit 280, the growth Air is supplied to the inside of the pipe (210).
  • the proliferating stem cell supplying step (S400) and the stem cell separation step (S500) supply of the proliferated stem cells to the centrifuge 300, and separation of the components of the stem cells in the centrifuge 300 Is done.
  • the proliferating stem cell supplying step (S400) the first extension pipe part 213 and the first supply pipe 110 are separated by a first opening and closing unit 241, the second extension pipe part 215 and the second.
  • the stem cells proliferated through the second extension pipe part 215 and the second supply pipe 310 are separated from the stem cell multiplier 200. It will be supplied to the centrifuge 300.
  • the stem cell separation step (S500) in the state in which the second extension tube part 215 and the second supply tube 310 are separated by the second opening and closing unit 242, by the centrifuge 300 Stem cells will be separated by component.
  • the stem cell extraction by the stem cell extractor 100, the proliferation of stem cells by the stem cell multiplier 200 and the separation of the stem cells by component centrifuge 300 It is done continuously.
  • the stem cell extraction step (S100) is initiated regardless of the completion of the stem cell growth step (S300).
  • the proliferating stem cell supply step (S400) is completed, regardless of the completion of the stem cell separation step (S500), the extracted stem cells and proliferation medium supply step (S200) is started. Therefore, in this embodiment, continuous stem cells can be proliferated, extracted, and separated by components.
  • FIG. 3 is a schematic view showing a stem cell continuous proliferation, extraction and separation system according to a second embodiment of the present invention.
  • reference numerals of FIG. 1 are used, and detailed description thereof will be omitted.
  • the stem cell continuous proliferation, extraction and separation system 2 further includes a tilting unit 290.
  • the tilting unit 290 tilts the growth tube 210, substantially the growth tube 210 and the growth vessel 220 at a predetermined tilting angle.
  • the 'tilting angle' will be set in a range in which the spiral tube portion 211 can be inclined at an angle of more than 0 ° and 45 ° or less with respect to the horizontal plane.

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Abstract

The present invention provides a device and a method for proliferating a stem cell, and a system and a method for continuously proliferating, extracting, and separating a stem cell. A stem cell proliferation device according to an embodiment of the present invention comprises: a proliferation pipe in which a stem cell is proliferated, and which comprises a helical pipe part formed in a helical shape, a first extended pipe part extending from one end of the helical pipe part so as to receive a stem cell extracted from the outside and a proliferation medium for the proliferation of the stem cell, and a second extended pipe part extending from the other end of the helical pipe part so as to supply the proliferated stem cell to the outside; a medium supply unit for supplying the proliferation medium to the proliferation pipe; a first opening/closing unit for selectively connecting/separating the first extended pipe part to/from a first supply pipe for supplying the extracted stem cell to the proliferation pipe; a second opening/closing unit for selectively connecting/separating the second extended pipe part to/from a second supply pipe for supplying the stem cell proliferated in the proliferation pipe; and a rotation-driving unit for rotating the proliferation pipe, wherein, when the extracted stem cell and the proliferation medium are supplied to the helical pipe part, the proliferation pipe is rotated by the rotation-driving unit in the state in which the first extended pipe part and the second extended pipe part are separated from the first supply pipe and the second supply pipe by the first opening/closing unit and the second opening/closing unit, respectively.

Description

[규칙 제26조에 의한 보정 30.08.2018]　줄기세포 증식기 및 증식 방법, 줄기세포 연속 증식, 추출 및 분리 시스템 및 방법[Revision 30.08.2018 by Rule 26] Stem Cell Proliferation and Proliferation Methods, Stem Cell Continuous Proliferation, Extraction and Separation Systems and Methods
본 발명은 줄기세포 증식기 및 증식 방법, 줄기세포 연속 증식, 추출 및 분리 시스템 및 방법에 관한 것이다.The present invention relates to stem cell proliferative phase and proliferation method, stem cell continuous proliferation, extraction and separation system and method.
줄기세포(Stem Cell)란, 뼈, 신경, 근육, 연골 등과 같은 다양한 신체 조직세포로의 분화가 가능한 것으로, 획득 방식에 따라서 수정란 배아줄기세포, 체세포 복제 배아줄기세포, 역분화유도 줄기세포, 성체줄기세포 등으로 구분될 수 있다.Stem cells are capable of differentiating into various body tissue cells such as bone, nerve, muscle, cartilage, etc., depending on the method of obtaining, embryonated embryonic stem cells, somatic cell cloned embryonic stem cells, inverted induced stem cells, and adults Stem cells and the like.
이중 성체줄기세포(Adult Stem Cell)의 경우에는, 성체의 지방, 골수, 제대혈 등으로부터 추출되는 것으로, 자가 이식의 경우에는 면역 거부 반응의 발생 가능성이 낮으므로, 안정적인 이식이 가능한 장점이 있다. 이와 같은 성체줄기세포의 경우에는, 성체의 지방 등으로부터 추출한 후 이를 증식하여 사용한다.In the case of the adult stem cells (Adult Stem Cell), which is extracted from the fat, bone marrow, umbilical cord blood, etc. of the adult, in the case of autologous transplantation is unlikely to generate an immune rejection reaction, there is an advantage that the stable transplantation. In the case of such adult stem cells, it is extracted from the fat of the adult and then used to proliferate it.
대한민국 등록특허공보 제1507621호(이하에서는, '선행특허문헌'이라고 칭함)에는 종래 기술에 의한 증식기가 개시되어 있다. 선행특허문헌을 참조하면, 종래에는, 가요성 재질의 배관(1)이 함유한 증식 배지에 의하여 배관(1)의 표면에서 생 세포가 생장하고, 클램프/게이트(3)(4)(5)에 의한 개폐에 따라서 배관(1)이 미사용 배양 배지(7)의 상류 영역, 사용된 배양 배지(15)의 하류 영역 및 상류 영역과 하류 영역 사이의 생장실 영역(10)으로 분할된다. 그리고 종래에는, 배관(1)이 이동, 즉 상류 영역-생장실 영역(10)-하류 영역으로 계속적으로 이동하면서 생 세포의 연속적인 생육이 이루어진다.Republic of Korea Patent No. 1507621 (hereinafter referred to as "prior patent document") discloses a multiplier according to the prior art. Referring to the prior patent document, conventionally, live cells grow on the surface of the pipe 1 by the growth medium contained in the flexible pipe 1, and the clamps / gates 3, 4, and 5 are applied. The pipe 1 is divided into an upstream region of the unused culture medium 7, a downstream region of the culture medium 15 used, and a growth chamber region 10 between the upstream region and the downstream region in accordance with opening and closing by the opening and closing process. And conventionally, continuous growth of live cells is achieved while the pipe 1 is continuously moved, ie, continuously moved to the upstream region-the growth chamber region 10-downstream region.
그러나 종래 기술에 의한 증식기에 의하면 다음과 같은 문제점이 발생된다.However, according to the multiplier according to the prior art, the following problems occur.
먼저, 종래에는, 별도로 추출된 세포를 증식기, 실질적으로 배관(1)에 공급하여야 한다. 따라서 종래에는, 실질적으로 세포의 추출 및 증식이 연속적으로 이루어지지 못하게 되는 단점이 발생한다. 나아가 종래에는, 추출된 세포를 배관(1)의 내부로 공급하는 과정에서 오염될 우려가 있다.First, conventionally, separately extracted cells should be supplied to the multiplier, substantially the pipe 1. Therefore, in the related art, there is a disadvantage in that the extraction and proliferation of the cells are substantially impossible. Furthermore, in the prior art, there is a fear of contamination in the process of supplying the extracted cells to the inside of the pipe (1).
또한 종래에는, 실질적으로 소정의 길이의 배관(1)을 사용한 세포의 증식이 완료되면, 새로운 배관(1)을 추가하여 공급하여야 한다. 따라서 종래에는, 계속적으로 배관(1)을 공급하여야 하고, 이를 위한 구성이 추가되어야 하는 불편이 따르게 된다.In addition, conventionally, when the proliferation of cells using the pipe 1 having a predetermined length is completed, a new pipe 1 must be added and supplied. Therefore, in the related art, it is necessary to continuously supply the pipe 1, and there is a inconvenience that a configuration for this needs to be added.
그리고 종래에는, 배관(1)의 영역이 클램프/게이트(3)(4)(5)에 의하여 개폐되어 각 영역이 분할된다. 따라서 종래에는, 클램프/게이트(3)(4)(5)에 의한 영역을 분할을 위하여 배관(1)이 가요성 재질로 성형되어야 하므로, 클램프/게이트(3)(4)(5)에 의한 개폐과정에서의 배관(1)의 손상이 우려될 수도 있다.And conventionally, the area | region of the piping 1 is opened and closed by clamp / gate (3) (4) (5), and each area | region is divided | segmented. Therefore, in the related art, since the pipe 1 has to be molded of a flexible material in order to divide the area by the clamps / gates 3, 4, 5, the clamps / gates 3, 4, 5 The pipe 1 may be damaged during the opening and closing process.
뿐만 아니라, 종래에는, 세포 증식을 위해서 관(1)이 상류 영역-생장실 영역(10)-하류 영역으로 순차적으로 구획되어야 하므로, 소정의 길이의 공간이 확보되어야 한다. 따라서 종래에는, 세포 증식기의 설치, 즉 세포 증식을 위한 공간이 불필요하게 소모되는 단점이 있다. In addition, conventionally, since the tube 1 must be sequentially partitioned into an upstream region-growth region 10-downstream region for cell proliferation, a predetermined length of space must be secured. Therefore, in the related art, there is a disadvantage that the space for installing the cell multiplier, that is, the cell proliferation is unnecessarily consumed.
본 발명은 상술한 바와 같은 종래 기술에 의한 문제점을 해결하기 위한 것으로, 본 발명의 목적은, 연속적인 줄기세포의 추출과 증식 및 증식된 세포에 대한 분리가 이루어질 수 있도록 구성되는 줄기세포 증식기 및 증식 방법, 줄기세포 연속 증식, 추출 및 분리 시스템 및 방법을 제공하는 것이다.The present invention is to solve the problems caused by the prior art as described above, an object of the present invention, the stem cell proliferative phase and proliferation configured to enable the extraction and proliferation of continuous stem cells and separation for the proliferated cells It is to provide a method, a stem cell continuous proliferation, extraction and separation system and method.
본 발명의 다른 목적은, 줄기세포의 오염을 방지할 수 있도록 구성되는 줄기세포 증식기 및 증식 방법, 줄기세포 연속 증식, 추출 및 분리 시스템 및 방법을 제공하는 것이다.Another object of the present invention is to provide a stem cell multiplier and proliferation method, a stem cell continuous proliferation, extraction and separation system and method configured to prevent the contamination of stem cells.
본 발명의 또 다른 목적은, 보다 간단하게 연속적인 줄기세포의 추출, 증식 및 분리가 가능하도록 구성되는 줄기세포 증식기 및 증식 방법, 줄기세포 연속 증식, 추출 및 분리 시스템 및 방법을 제공하는 것이다.Still another object of the present invention is to provide a stem cell multiplier and proliferation method, a stem cell continuous proliferation, extraction and separation system and method, which are configured to more simply extract, proliferate, and separate continuous stem cells.
본 발명의 또 다른 목적은, 내구성의 확보가 가능하도록 구성되는 줄기세포 증식기 및 증식 방법, 줄기세포 연속 증식, 추출 및 분리 시스템 및 방법을 제공하는 것이다.Still another object of the present invention is to provide a stem cell multiplier and a proliferation method, a stem cell continuous proliferation, an extraction and separation system and a method configured to ensure durability.
본 발명의 또 다른 목적은, 보다 컴팩트하게 구성되는 줄기세포 증식기 및 증식 방법, 줄기세포 연속 증식, 추출 및 분리 시스템 및 방법을 제공하는 것이다.Still another object of the present invention is to provide a stem cell multiplier and a method of proliferation, a stem cell continuous proliferation, an extraction and separation system and a method which are more compactly configured.
상술한 바와 같은 목적을 달성하기 위한 본 발명의 실시예에 의한 줄기세포 증식기의 일 양태는, 나선 형상으로 형성되는 나선관부, 상기 나선부의 일단에서 연장되어 외부로부터 추출된 줄기세포 및 줄기세포의 증식을 위한 증식 배지를 공급받는 제1연장관부 및 상기 나선부의 타단에서 연장되어 외부로 증식된 줄기세포를 공급하는 제2연장관부를 포함하고, 줄기세포의 증식이 이루어지는 증식관; 상기 증식관에 증식 배지를 공급하는 배지 공급 유닛; 상기 제1연장관부를 상기 증식관으로 추출된 줄기세포를 공급하기 위한 제1공급관과 선택적으로 연통/분리시키는 제1개폐 유닛; 상기 제2연장관부를 상기 증식관에서 증식된 줄기세포를 공급하기 위한 제2공급관과 선택적으로 연통/분리시키는 제2개폐 유닛; 및 상기 증식관을 회전시키는 회전 구동 유닛; 을 포함하고, 상기 나선관부에 추출된 줄기세포 및 증식 배지가 공급되면, 상기 제1 및 제2개폐 유닛에 의하여 상기 제1 및 제2연장관부와 상기 제1 및 제2공급관이 분리된 상태에서, 상기 회전 구동 유닛에 의하여 상기 증식관이 회전한다.One aspect of the stem cell multiplier according to an embodiment of the present invention for achieving the above object is a spiral tube portion formed in a spiral shape, the proliferation of stem cells and stem cells extracted from the outside extending from one end of the spiral portion A proliferation tube including a first extension tube part receiving the proliferation medium for supplying the second extension tube part extending from the other end of the spiral part and supplying stem cells proliferated to the outside; A medium supply unit supplying a growth medium to the growth tube; A first opening / closing unit for selectively communicating / separating the first extension tube part with the first supply tube for supplying the stem cells extracted into the growth tube; A second opening / closing unit for selectively communicating / separating the second extension tube part with a second supply tube for supplying stem cells proliferated in the growth tube; And a rotation drive unit for rotating the propagation tube. When the stem cells and the proliferation medium extracted from the spiral tube part is supplied, the first and second extension tube portion and the first and second supply tube in the state separated by the first and second opening and closing unit The growth tube is rotated by the rotation driving unit.
본 발명의 실시예에 의한 줄기세포 증식기의 일 양태에서, 상기 증식관은, 상기 제1연장관부에 인접하는 상기 나선관부의 일측 또는 상기 제1연장관부의 일측에 일단이 연결되고, 상기 제2연장관부에 인접하는 상기 나선관부의 다른 일측 또는 상기 제2연장관부의 일측에 타단이 연결되는 순환관부를 더 포함하고, 상기 제1 및 제2개폐 유닛에 의하여 상기 제1 및 제2연장관부와 상기 제1 및 제2공급관이 분리된 상태에서, 줄기세포 및 증식 배지가 상기 나선관부를 순환하도록 상기 나선관의 타단에서 일단으로 줄기세포 및 증식 배지를 압송하는 순환 펌프를 더 포함한다.In one aspect of the stem cell multiplier according to an embodiment of the present invention, the proliferation tube, one end is connected to one side of the spiral tube portion or one side of the first extension tube portion adjacent to the first extension tube portion, the second extension Further comprising a circulation pipe portion having the other end connected to the other side of the spiral tube portion adjacent to the tube portion or one side of the second extension tube portion, the first and second extension tube portion and the first by the first and second opening and closing unit And a circulation pump for pumping the stem cells and the growth medium from one end of the spiral tube to the other end of the spiral tube so that the stem cells and the growth medium circulate the spiral tube in a state in which the first and second supply pipes are separated.
본 발명의 실시예에 의한 줄기세포 증식기의 일 양태에서, 적어도 상기 나선관부는, 수평면에 대하여 0° 초과 및 45°이하의 각도로 경사지게 배치된다.In one aspect of the stem cell multiplier according to an embodiment of the present invention, at least the spiral tube portion is disposed to be inclined at an angle of more than 0 ° and less than 45 ° with respect to the horizontal plane.
본 발명의 실시예에 의한 줄기세포 증식기의 일 양태는, 상기 증식관이 그 내부에 배치되고, 상기 회전 구동 유닛에 의하여 상기 증식관과 함께 회전하는 증식 용기를 더 포함한다. One aspect of the stem cell multiplier according to an embodiment of the present invention, the growth tube is further disposed therein, further comprises a growth vessel that rotates with the growth tube by the rotation drive unit.
본 발명의 실시예에 의한 줄기세포 증식기의 일 양태는, 상기 증식관에서의 줄기세포의 증식을 위한 온도를 조절하는 온도 조절 유닛을 더 포함한다.One aspect of the stem cell multiplier according to an embodiment of the present invention further comprises a temperature control unit for controlling the temperature for the proliferation of stem cells in the growth tube.
본 발명의 실시예에 의한 줄기세포 증식기의 일 양태는, 상기 증식관으로 공기를 공급하는 공기 공급 유닛을 더 포함한다.One aspect of the stem cell multiplier according to an embodiment of the present invention further comprises an air supply unit for supplying air to the growth tube.
본 발명의 실시예에 의한 줄기세포 증식 방법의 일 양태는, 제 1 항의 줄기세포 증식기를 사용한 줄기세포 증식 방법에 있어서: 추출된 줄기세포 및 줄기세포의 증식을 위한 증식 배지가 상기 증식관에 공급되는 추출 줄기세포 및 증식 배지 공급 단계; 및 줄기세포가 상기 증식관의 내부에서 증식되는 줄기세포 증식 단계; 를 포함한다.One embodiment of the stem cell proliferation method according to an embodiment of the present invention, in the stem cell proliferation method using the stem cell proliferator of claim 1: The proliferation medium for proliferation of the extracted stem cells and stem cells is supplied to the growth tube Supplying the extracted stem cells and the growth medium; And stem cell proliferation step in which stem cells proliferate inside the growth tube; It includes.
본 발명의 실시예에 의한 줄기세포 증식 방법의 일 양태에서, 상기 추출 줄기세포 및 증식 배지 공급 단계에서, 상기 제1개폐 유닛에 의하여 상기 제1연장관부 및 제1공급관이 연통되고, 상기 제2개폐 유닛에 의하여 상기 제2연장관부 및 제2공급관이 분리된 상태에서 상기 제1연장관부 및 제1공급관을 통하여 추출된 줄기세포 및 증식 배지가 상기 증식관으로 공급된다.In one aspect of the stem cell proliferation method according to an embodiment of the present invention, in the extracting stem cells and proliferation medium supplying step, the first extension tube portion and the first supply tube is communicated by the first opening and closing unit, the second The stem cells and the proliferation medium extracted through the first extension pipe part and the first supply pipe in a state where the second extension pipe part and the second supply pipe are separated by the opening / closing unit are supplied to the growth pipe.
본 발명의 실시예에 의한 줄기세포 증식 방법의 일 양태에서, 상기 줄기세포 증식 단계에서, 상기 회전 구동 유닛이, 상기 제1 및 제2개폐 유닛에 의하여 상기 제1 및 제2연장관부와 상기 제1 및 제2공급관이 분리된 상태에서, 상기 증식관을 회전시킨다.In one aspect of the stem cell proliferation method according to an embodiment of the present invention, in the stem cell proliferation step, the rotation drive unit, the first and second extension tube portion and the first by the first and second opening and closing unit The propagation tube is rotated while the first and second supply tubes are separated.
본 발명의 실시예에 의한 줄기세포 증식 방법의 일 양태에서, 상기 줄기세포 증식 단계에서, 순환 펌프가, 줄기세포 및 증식 배지가 상기 증식관을 순환하도록 압송한다.In one aspect of the stem cell proliferation method according to an embodiment of the present invention, in the stem cell proliferation step, a circulation pump pumps the stem cells and the proliferation medium to circulate the proliferation tube.
본 발명의 실시예에 의한 줄기세포 증식 방법의 일 양태에서, 상기 줄기세포 증식 단계에서, 온도 조절 유닛이, 상기 증식관에서의 줄기세포의 증식을 위한 온도를 조절하고, 공기 공급 유닛이, 상기 증식관의 내부로 공기를 공급한다.In one aspect of the stem cell proliferation method according to an embodiment of the present invention, in the stem cell proliferation step, the temperature control unit, the temperature for the proliferation of stem cells in the growth tube, the air supply unit, Supply air into the growth tube.
본 발명의 실시예에 의한 줄기세포 연속 증식, 추출 및 분리 시스템의 일 양태는, 시료로부터 줄기세포를 추출하는 줄기세포 추출기; 상기 줄기세포 추출기로부터 추출된 줄기세포를 공급받아서 증식하는 제 1 항 내지 제 6 항 중 어느 한 항의 줄기세포 증식기; 및 상기 줄기세포 증식기로부터 증식된 줄기세포를 공급받아서 성분별로 분리하는 원심 분리기; 를 포함한다.One embodiment of the stem cell continuous proliferation, extraction and separation system according to an embodiment of the present invention, the stem cell extractor for extracting stem cells from the sample; A stem cell multiplier according to any one of claims 1 to 6 for receiving and proliferating stem cells extracted from the stem cell extractor; And a centrifugal separator for receiving the stem cells proliferated from the stem cell multiplier to separate by component; It includes.
본 발명의 실시예에 의한 줄기세포 추출, 증식 및 분리 시스템의 일 양태에서, 상기 줄기세포 추출기는, 상기 제1연장관부와 연통되어 추출된 줄기세포를 상기 줄기세포 증식기에 공급하는 제1공급관을 포함하고, 상기 원심분리기는, 상기 제2연장관부와 연통되어 증식된 줄기세포를 상기 줄기세포 증식기로부터 공급받는 제2공급관을 포함한다. In one aspect of the stem cell extraction, proliferation and separation system according to an embodiment of the present invention, the stem cell extractor, the first supply pipe for supplying the stem cell proliferation to the stem cell multiplier in communication with the first extension tube portion; The centrifuge includes a second supply pipe that receives stem cells proliferated in communication with the second extension tube part from the stem cell multiplier.
본 발명의 실시예에 의한 줄기세포 추출, 증식 및 분리 시스템의 일 양태에서, 상기 제1개폐 유닛에 의하여 상기 제1연장관부 및 제1공급관이 연통된 상태에서, 상기 줄기세포 추출기에 의하여 추출된 줄기세포 및 상기 증식 배지 공급 유닛에 의하여 공급되는 증식 배지를 상기 증식관으로 압송하는 제1공급 펌프를 더 포함한다.In one aspect of the stem cell extraction, proliferation and separation system according to an embodiment of the present invention, in the state in which the first extension tube and the first supply tube in communication with the first opening and closing unit, extracted by the stem cell extractor And a first supply pump for pumping the stem cells and the growth medium supplied by the growth medium supply unit to the growth tube.
본 발명의 실시예에 의한 줄기세포 추출, 증식 및 분리 시스템의 일 양태에서, 상기 제2개폐 유닛에 의하여 상기 제2연장관부 및 제2공급관이 연통된 상태에서, 상기 줄기세포 증식기에 의하여 증식된 줄기세포를 상기 원심 분리기로 압송하는 제2공급 펌프를 더 포함한다.In one aspect of the stem cell extraction, proliferation and separation system according to an embodiment of the present invention, in the state in which the second extension tube portion and the second supply tube is in communication by the second opening and closing unit, it is propagated by the stem cell multiplier It further comprises a second feed pump for pumping stem cells into the centrifuge.
본 발명의 실시예에 의한 줄기세포 연속 증식, 추출 및 분리 방법의 일 양태는, 제 12 항의 줄기세포 연속 증식, 추출 및 분리 시스템을 사용하여 줄기세포를 연속 증식, 추출 및 분리하는 방법에 있어서: 줄기세포가, 상기 줄기세포 추출기에 의하여 추출되는 줄기세포 추출 단계; 상기 줄기세포 추출 단계에서 추출된 줄기세포 및 줄기세포의 증식을 위한 증식 배지가 상기 증식관에 공급되는 추출 줄기세포 및 증식 배지 공급 단계; 상기 추출 줄기세포 및 증식 배지 공급 단계에서 상기 증식관에 공급된 줄기세포가 상기 증식관의 내부에서 증식되는 줄기세포 증식 단계; 상기 줄기세포 증식 단계에서 증식된 줄기세포가 상기 원심 분리기에 공급되는 증식 줄기세포 공급 단계; 및 상기 증식 줄기세포 공급 단계에서 공급된 줄기세포가 상기 원심 분리기에 의하여 성분별로 분리되는 줄기세포 분리 단계; 를 포함한다.In one aspect of the method for continuously proliferating, extracting and separating stem cells according to an embodiment of the present invention, the method for continuously proliferating, extracting and separating stem cells using the stem cell continuous proliferation, extraction and separation system of claim 12: Stem cells, the stem cell extraction step is extracted by the stem cell extractor; Extracting stem cells and a growth medium supplying a proliferation medium for proliferation of stem cells and stem cells extracted in the stem cell extraction step to the growth tube; A stem cell proliferation step of proliferating the stem cells supplied to the growth tube in the extracting stem cells and the growth medium supplying step in the growth tube; A proliferating stem cell supplying step of supplying the stem cells proliferated in the stem cell proliferation step to the centrifuge; And a stem cell separation step of separating the stem cells supplied in the proliferating stem cell supply step by component by the centrifuge. It includes.
본 발명의 실시예에 의한 줄기세포 연속 증식, 추출 및 분리 방법의 일 양태에서, 상기 추출 줄기세포 및 증식 배지 공급 단계에서, 상기 제1개폐 유닛에 의하여 상기 제1연장관부 및 제1공급관이 연통되고, 상기 제2개폐 유닛에 의하여 상기 제2연장관부 및 제2공급관이 분리된 상태에서, 상기 제1연장관부 및 제1공급관을 통하여 상기 줄기세포 추출기에서 추출된 줄기세포 및 상기 증식 배지 공급 유닛으로부터의 증식 배지가 상기 증식관으로 공급된다.In one aspect of the stem cell continuous growth, extraction and separation method according to an embodiment of the present invention, in the step of supplying the extracted stem cells and growth medium, the first extension tube and the first supply tube is communicated by the first opening and closing unit The stem cells and the proliferation medium supply unit extracted from the stem cell extractor through the first extension pipe part and the first supply pipe in a state in which the second extension pipe part and the second supply pipe are separated by the second opening and closing unit. Propagation medium from is supplied to the growth vessel.
본 발명의 실시예에 의한 줄기세포 연속 증식, 추출 및 분리 방법의 일 양태에서, 상기 줄기세포 증식 단계에서, 상기 회전 구동 유닛이, 상기 제1 및 제2개폐 유닛에 의하여 상기 제1 및 제2연장관부와 상기 제1 및 제2공급관이 분리된 상태에서, 상기 증식관을 회전시킨다. In one aspect of the stem cell continuous growth, extraction and separation method according to an embodiment of the present invention, in the stem cell proliferation step, the rotation drive unit, the first and second opening and closing unit by the first and second opening and closing unit The propagation tube is rotated while the extension tube portion and the first and second supply tubes are separated.
본 발명의 실시예에 의한 줄기세포 연속 증식, 추출 및 분리 방법의 일 양태에서, 상기 줄기세포 증식 단계에서, 순환 펌프가, 상기 제1 및 제2개폐 유닛에 의하여 상기 제1 및 제2연장관부와 상기 제1 및 제2공급관이 분리된 상태에서, 줄기세포 및 증식 배지가 상기 증식관을 순환하도록 압송한다.In one aspect of the stem cell continuous proliferation, extraction and separation method according to an embodiment of the present invention, in the stem cell proliferation step, the circulation pump, the first and second extension tube by the first and second opening and closing unit In the state where the first and second supply pipes are separated, stem cells and proliferation medium are pumped to circulate the proliferation pipes.
본 발명의 실시예에 의한 줄기세포 연속 증식, 추출 및 분리 방법의 일 양태에서, 상기 추출 줄기세포 및 증식 배지 공급 단계가 완료되면, 상기 줄기세포 증식 단계의 완료와 무관하게 상기 줄기세포 추출 단계가 개시된다.In one aspect of the method for continuously proliferating, extracting and separating stem cells according to an embodiment of the present invention, when the extracting stem cells and the proliferation medium supply step are completed, the stem cell extraction step may be performed regardless of the completion of the stem cell proliferation step. Is initiated.
본 발명의 실시예에 의한 줄기세포 연속 증식, 추출 및 분리 방법의 일 양태에서, 상기 증식 줄기세포 공급 단계가 완료되면, 상기 줄기세포 분리 단계의 완료와 무관하게 상기 추출 줄기세포 및 증식 배지 공급 단계가 개시된다.In one aspect of the method for continuously proliferating, extracting and separating stem cells according to an embodiment of the present invention, when the proliferating stem cell supplying step is completed, the extracting stem cells and proliferation medium supplying step are independent of completion of the stem cell separation step. Is disclosed.
본 발명의 실시예에 의한 줄기세포 증식기 및 증식 방법, 줄기세포 연속 증식, 추출 및 분리 시스템 및 방법에 의하면 다음과 같은 효과를 기대할 수 있다.According to the stem cell proliferative phase and the proliferation method, the stem cell continuous proliferation, extraction and separation system and method according to an embodiment of the present invention can be expected the following effects.
먼저 본 발명의 실시예에서는, 줄기세포의 추출, 증식 및 성분별 분리가 연속적으로 이루어진다. 따라서 본 발명의 실시예 의하면, 보다 신속하게 줄기세포의 추출, 증식 및 성분별 분리가 이루어질 수 있다.First, in the embodiment of the present invention, the extraction, proliferation and separation of the components of the stem cells are made continuously. Therefore, according to an embodiment of the present invention, stem cells can be more quickly extracted, expanded, and separated by components.
또한 본 발명의 실시예에서는, 줄기세포 추출기에서 추출된 줄기세포가 관을 통하여 줄기세포 증식기로 직접 공급되고, 줄기세포 증식기에서 증식된 줄기세포가 관을 통하여 원심 분리기로 직접 공급된다. 따라서 본 발명의 실시예에 의하면, 줄기세포 추출기, 줄기세포 증식기 및 원심 분리기 사이에서 줄기세포의 이동 과정에서의 오염을 방지할 수 있다.In addition, in the embodiment of the present invention, the stem cells extracted from the stem cell extractor is directly supplied to the stem cell proliferator through the tube, the stem cells proliferated in the stem cell proliferator are directly supplied to the centrifuge through the tube. Therefore, according to the embodiment of the present invention, it is possible to prevent contamination during the movement of stem cells between the stem cell extractor, stem cell multiplier and centrifuge.
그리고 본 발명의 실시예에서는, 줄기세포 추출기와 줄기세포 증식기 사이 및 줄기세포 증식기와 원심 분리기 사이의 관을 선택적으로 연통/분리함으로써, 줄기세포의 추출, 증식 및 성분별 분리가 연속적으로 이루어진다. 따라서 본 발명의 실시예에 의하면, 보다 간단한 구성으로 연속적인 줄기세포의 추출, 증식 및 성분별 분리가 이루어질 수 있다.In an embodiment of the present invention, by selectively communicating / separating the tube between the stem cell extractor and the stem cell multiplier and between the stem cell multiplier and the centrifuge, the extraction, proliferation and separation of the stem cells are continuously performed. Therefore, according to the embodiment of the present invention, it is possible to extract, proliferate, and separate components by successive stem cells in a simpler configuration.
나아가 본 발명의 실시예에서는, 줄기세포의 증식이 이루어지는 증식관의 회전 및 증식관 내부에서의 줄기세포 및 증식 배지의 순환에 의하여 줄기세포의 증식이 이루어진다. 따라서 본 발명의 실시예에 의하면, 소정의 강성을 갖는 증식관의 사용 가능하므로, 시스템의 내구성을 확보할 수 있게 된다.Furthermore, in the embodiment of the present invention, stem cells are proliferated by the rotation of the proliferative tube through which the stem cells are proliferated and the circulation of the stem cells and the proliferation medium in the proliferating tube. Therefore, according to the embodiment of the present invention, it is possible to use a propagation tube having a predetermined rigidity, thereby ensuring the durability of the system.
또한 본 발명의 실시예에서는, 증식관의 일부가 나선 형상으로 형성됨으로써, 줄기세포 증식기의 전체적으로 크기 및 체적이 감소될 수 있다. 따라서 본 발명의 실시예에 의하면, 상대적으로 작은 크기 및 체적의 공간에 대한 온도 등의 조절만 이루어짐으로써, 보다 용이하게 줄기세포의 증식을 위한 환경 조절이 가능해진다.In addition, in the embodiment of the present invention, by forming a portion of the growth tube in a spiral shape, the overall size and volume of the stem cell proliferative phase can be reduced. Therefore, according to the embodiment of the present invention, by adjusting only the temperature of the relatively small size and volume of the space, it is possible to more easily control the environment for the proliferation of stem cells.
도 1은 본 발명의 제1실시예에 의한 줄기세포 연속 증식, 추출 및 분리 시스템을 개략적으로 보인 구성도.1 is a schematic view showing a stem cell continuous proliferation, extraction and separation system according to a first embodiment of the present invention.
도 2는 본 발명의 제1실시예에 의한 줄기세포 연속 증식, 추출 및 분리 방법을 보인 플로우 챠트.Figure 2 is a flow chart showing the stem cell continuous proliferation, extraction and separation method according to a first embodiment of the present invention.
도 3은 본 발명의 제2실시예에 의한 줄기세포 연속 증식, 추출 및 분리 시스템을 개략적으로 보인 구성도.Figure 3 is a schematic diagram showing a stem cell continuous proliferation, extraction and separation system according to a second embodiment of the present invention.
이하에서는 본 발명의 제1실시예에 의한 줄기세포 연속 증식, 추출 및 분리 시스템을 첨부된 도면을 참조하여 보다 상세하게 설명한다.Hereinafter, the stem cell continuous proliferation, extraction and separation system according to the first embodiment of the present invention will be described in more detail with reference to the accompanying drawings.
도 1은 본 발명의 제1실시예에 의한 줄기세포 연속 증식, 추출 및 분리 시스템을 개략적으로 보인 구성도이다.1 is a schematic view showing a stem cell continuous proliferation, extraction and separation system according to a first embodiment of the present invention.
도 1을 참조하면, 본 실시예에 의한 줄기세포 연속 증식, 추출 및 분리 시스템(1)은, 줄기세포 추출기(100), 줄기세포 증식기(200), 원심 분리기(300) 및 제1 및 제2공급 펌프(410)(420)를 포함한다. 본 실시예에 의한 줄기세포 연속 증식, 추출 및 분리 시스템(1)에서는, 특히, 줄기세포의 증식, 추출 및 성분별 분리가 연속적으로 이루어진다.Referring to Figure 1, the stem cell continuous proliferation, extraction and separation system 1 according to the present embodiment, stem cell extractor 100, stem cell multiplier 200, centrifuge 300 and the first and second Feed pumps 410 and 420. In the stem cell continuous proliferation, extraction and separation system 1 according to the present embodiment, in particular, the proliferation, extraction and separation of the stem cells are carried out continuously.
보다 상세하게는, 상기 줄기세포 추출기(100)는, 시료로부터 줄기세포를 추출한다. 예를 들면, 상기 줄기세포 추출기(100)는, 성체의 지방, 골수, 제대혈 등으로부터 줄기세포, 즉 성체줄기세포를 추출할 수 있다. 상기 줄기세포 추출기(100)는, 공지된 다양한 종류의 추출 방법으로 줄기세포를 추출할 수 있다. 상기 줄기세포 추출기(100)는, 추출된 줄기세포를 상기 줄기세포 증식기(200)에 공급하는 제1공급관(110)을 포함한다.In more detail, the stem cell extractor 100 extracts stem cells from a sample. For example, the stem cell extractor 100 may extract stem cells, that is, adult stem cells from adult fat, bone marrow, umbilical cord blood, and the like. The stem cell extractor 100 may extract stem cells by various kinds of known extraction methods. The stem cell extractor 100 includes a first supply pipe 110 for supplying the extracted stem cells to the stem cell multiplier 200.
그리고 상기 줄기세포 증식기(200)는, 상기 줄기세포 추출기(100)로부터 추출된 줄기세포를 공급받아서 증식한다. 상기 줄기세포 증식기(200)는, 증식관(210), 증식 용기(220), 배지 공급 유닛(230), 제1 및 제2개폐 유닛(241)(242), 회전 구동 유닛(250), 순환 펌프(260), 온도 조절 유닛(270) 및 공기 공급 유닛(280)을 포함한다.The stem cell multiplier 200 receives and proliferates stem cells extracted from the stem cell extractor 100. The stem cell multiplier 200, the growth tube 210, growth vessel 220, medium supply unit 230, the first and second opening and closing unit 241, 242, rotation drive unit 250, circulation A pump 260, a temperature control unit 270 and an air supply unit 280.
보다 상세하게는, 상기 증식관(210)은, 줄기세포의 증식이 이루어지는 곳이다. 본 실시예에서는, 상기 증식관(210)이 나선관부(211), 제1 및 제2연장관부(213)(215) 및 순환관부(217)를 포함한다. More specifically, the growth tube 210 is a place where stem cells proliferate. In the present embodiment, the growth tube 210 includes a spiral tube portion 211, the first and second extension tube portion 213, 215 and the circulation tube portion 217.
상기 나선관부(211)는, 전체적으로 나선형상으로 형성된다. 실질적으로 상기 증식관(210)의 내부로 공급되는 줄기세포는, 상기 나선관부(211)의 내면에 착상된 상태로 증식 배지에 의하여 증식될 것이다.The spiral pipe portion 211 is formed in a spiral shape as a whole. Substantially the stem cells supplied into the growth tube 210 will be proliferated by the growth medium in a state implanted in the inner surface of the spiral tube portion 211.
그리고 상기 제1연장관부(213)는, 상대적으로 상방에 위치되는 상기 나선관부(211)의 일단에서 연장되어 상기 줄기세포 추출기(100)에서 추출된 줄기세포 및 줄기세포의 증식을 위한 증식 배지를 공급받는다. 상기 제2연장관부(215)는, 상대적으로 하방에 위치되는 상기 나선관부(211)의 타단에서 연장되어 상기 줄기세포 증식기(200), 실질적으로 상기 증식관(210)에서 증식된 줄기세포를 상기 원심 분리기(300)에 공급한다. The first extension tube portion 213 extends from one end of the spiral tube portion 211 positioned relatively upward to provide a growth medium for proliferation of stem cells and stem cells extracted from the stem cell extractor 100. To be supplied. The second extension tube portion 215 extends from the other end of the spiral tube portion 211 positioned relatively downward to the stem cell multiplier 200 and substantially the stem cells proliferated in the growth tube 210. Supply to the centrifuge (300).
한편 상기 순환관부(217)는, 줄기세포 및 증식 배지, 특히, 줄기세포가 상기 나선관부(211)의 표면에 착상된 이후에는 주로 증식 배지가 상기 나선관부(211)를 순환할 수 있도록 하기 위한 것이다. 실질적으로 상기 순환관부(217)의 내부에는 상기 나선관부(211)의 타단에서 일단으로 압송되는 줄기세포 및 증식 배지가 유동된다. 이를 위하여 상기 순환관부(217)의 일단은 상기 제1연장관부(213)에 인접하는 상기 나선관부(211)의 일측 또는 상기 제1연장관부(213)의 일측에 연결되고, 상기 순환관부(217)의 타단은 상기 제2연장관부(215)에 인접하는 상기 나선관부(211)의 다른 일측 또는 상기 제2연장관부(215)의 일측에 타단이 연결된다. 물론, 상기 순환관부(217)의 내면에도 줄기세포가 착상될 수 있을 것이다.On the other hand, the circulation pipe 217, the stem cells and proliferation medium, in particular, after the stem cells are implanted on the surface of the spiral pipe portion 211 mainly for the growth medium to circulate the spiral pipe portion 211 will be. Substantially inside the circulation pipe 217 flows stem cells and proliferation medium, which are pushed to one end from the other end of the spiral pipe 211. To this end, one end of the circulation pipe part 217 is connected to one side of the spiral pipe part 211 adjacent to the first extension pipe part 213 or to one side of the first extension pipe part 213, and the circulation pipe part 217 The other end of) is connected to the other end of the spiral tube portion 211 adjacent to the second extension tube portion 215 or to one side of the second extension tube portion 215. Of course, stem cells may also be implanted into the inner surface of the circulation tube 217.
특히, 본 실시예에서는, 상기 나선관부(211)가, 수평면에 대하여 0° 초과 및 45°이하의 각도로 경사지게 배치된다. 이는, 줄기세포의 착상과 줄기세포 및 증식 배지의 순환이 효율적으로 이루어지도록 하기 위함이다. 보다 상세하게는, 상기 나선관부(211)가 수평면에 대하여 45°초과의 각도로 배치되는 경우에는, 상기 나선관부(211)의 내부에서 줄기세포가 상대적으로 과도하게 빠른 속도로 유동함으로써, 줄기세포의 착상이 충분하게 이루어질 수 없게된다. 반대로 상기 나선관부(211)가 수평면에 대하여 0°, 즉 수평면에 평행하게 배치되는 경우에는, 상기 나선관부(211)의 내부에서 줄기세포 및 증식 배지가 상대적으로 과도하게 느린 속도로 유동됨으로써, 줄기세포가 상기 나선관부(211)의 내면에 국부적으로 착상되거나 증식 배지가 충분한 속도로 유동되지 못하게 될 수 있다. 물론, 후술할 바와 같이, 상기 순환 펌프(260)의 압송에 의하여 상기 나선관부(211) 및 순환관부(217)에서의 줄기세포 및 증식 배지의 순환이 이루어지지만, 상기 순환 펌프(260)는, 상대적으로 하방에 위치되는 상기 나선관부(211)의 타단에서 상대적으로 상방에 위치되는 상기 나선관부(211)의 일단으로 줄기세포 및 증식 배지를 강제적으로 유동시키는 것이고, 실질적으로 상기 나선관부(211)의 내부에서의 줄기세포 및 증식 배지의 유동은 자중에 의하여 상대적으로 저속으로 이루어진다. 따라서 본 실시예에서는, 상기 나선관부(211)의 수평면에 대한 경사 각도를 0° 초과 및 45°이하로 한정함으로써, 줄기세포의 착상 및 증식 배지의 순환이 줄기세포의 증식을 위한 충분한 수준으로 이루어지도록 한다.In particular, in the present embodiment, the spiral pipe portion 211 is disposed to be inclined at an angle of more than 0 ° and 45 ° or less with respect to the horizontal plane. This is to ensure efficient circulation of stem cells and stem cells and proliferation medium. In more detail, when the spiral tube portion 211 is disposed at an angle of more than 45 ° with respect to the horizontal plane, the stem cells flow in the spiral tube portion 211 at an excessively high speed so that the stem cells flow. The idea of not being made enough. On the contrary, when the spiral tube portion 211 is disposed at 0 ° with respect to the horizontal plane, that is, parallel to the horizontal plane, the stem cells and the proliferation medium flow in the spiral tube portion 211 at a relatively excessively slow speed, thereby causing the stem Cells may be locally implanted on the inner surface of the spiral tube portion 211 or proliferation medium may not flow at a sufficient speed. Of course, as will be described later, the circulation of the stem cells and the growth medium in the spiral pipe portion 211 and the circulation pipe portion 217 by the pressure of the circulation pump 260, but the circulation pump 260, The stem cell and the proliferation medium are forcibly flowed from one end of the spiral tube portion 211 positioned relatively upward from the other end of the spiral tube portion 211 positioned relatively downward, and substantially the spiral tube portion 211 The flow of stem cells and proliferation medium in the inside is relatively slow due to its own weight. Therefore, in this embodiment, by limiting the inclination angle to the horizontal plane of the spiral tube portion 211 to more than 0 ° and less than 45 °, the implantation of stem cells and circulation of the growth medium is made to a sufficient level for the proliferation of stem cells To lose.
또한 본 실시예에서는, 줄기세포의 착상을 유도하고 증식 과정에서 줄기세포의 독성 생성을 방지하기 위하여 상기 증식관(210)의 내면에 코팅층(미도시)이 구비될 수 있다. 예를 들면, 줄기세포의 착상 유도 및 독성 생성 방지를 위하여, GSNO(nitroso-glutathione), CysNO(nitroso-cysteine)을 함유하는 히아루론산(hyaluronic acid) 또는 하이드록시-아파타이트(hydroxy-apatite), 하이드로젤(hydrogel) 및 매생이다당류(해조류 유래SPS-CF: marine algalsulphated polysaccharides of Capsosiphon Fulvescens)가 상기 증식관(210)의 내면에 코팅될 수 있다. In addition, in the present embodiment, a coating layer (not shown) may be provided on the inner surface of the growth tube 210 in order to induce the implantation of stem cells and to prevent toxic generation of stem cells in the proliferation process. For example, hyaluronic acid or hydroxy-apatite containing a nitroso-glutathione (GSNO), nitroso-cysteine (cysNO), or hydroxy-apatite (hydrogel) to prevent stem cell implantation and toxicity generation. Hydrogel and parasitic polysaccharides (SPS-CF: marine algalsulphated polysaccharides of Capsosiphon Fulvescens ) may be coated on the inner surface of the growth tube 210.
한편 상기 증식 용기(220)는, 대략 상면이 개방되는 중공의 원통 형상으로 형성될 수 있다. 그리고 상기 증식 용기(220)는, 그 내부에 상기 증식관(210)이 배치된 상태에서, 회전함으로써, 실질적으로 상기 증식관(210)과 함께 회전하게 된다.On the other hand, the growth vessel 220 may be formed in a hollow cylindrical shape of which the upper surface is approximately open. The growth vessel 220 is rotated in a state where the growth vessel 210 is disposed therein, thereby substantially rotating together with the growth vessel 210.
그리고 상기 증식 배지 공급 유닛(230)은, 상기 증식관(210)에 증식 배지를 공급한다. 상기 증식 배지 공급 유닛(230)은, 예를 들면, 상기 제1공급관(110)에 연결될 수 있다. 따라서 상기 증식 배지 공급 유닛(230)에 의하여 공급되는 증식 배지는, 상기 줄기세포 추출기(100)에서 추출된 줄기세포와 함께 상기 증식관(210)에 공급될 수 있다. 이때 줄기세포의 산화를 방지하기 위하여 GSH(Glutathione), GSNO(nitroso-glutathione) 및 CysNO(nitroso-cysteine) 중 어느 하나가 상기 증식관(210)에 공급되는 증식 배지에 포함될 수 있다.The growth medium supply unit 230 supplies a growth medium to the growth tube 210. The growth medium supply unit 230 may be connected to, for example, the first supply pipe 110. Therefore, the growth medium supplied by the growth medium supply unit 230 may be supplied to the growth tube 210 together with the stem cells extracted from the stem cell extractor 100. In this case, any one of GSH (Glutathione), GSNO (nitroso-glutathione), and CysNO (nitroso-cysteine) may be included in the growth medium supplied to the growth tube 210 to prevent the oxidation of stem cells.
상기 제1개폐 유닛(241)은, 상기 제1연장관부(213) 및 제1공급관(110)을 선택적으로 연통/분리시킨다. 그리고 상기 제2개폐 유닛(242)은, 상기 제2연장관부(215) 및 후술할 제2공급관(310)을 선택적으로 연통/분리시킨다. 실질적으로 상기 제1개폐 유닛(241)에 의하여 상기 제1연장관부(213) 및 제1공급관(110)을 통한 추출된 줄기세포 및 증식 배지의 공급이 선택적으로 이루어진다. 그리고 상기 제2개폐 유닛(242)에 의하여 상기 제2연장관부(215) 및 제2공급관(310)을 통한 증식된 줄기세포의 공급이 선택적으로 이루어진다. 본 실시예에서는, 상기 제1개폐 유닛(241)이 상기 제1연장관부(213) 및 제1공급관(110)의 단부를 각각 개폐하는 한쌍의 제1밸브(241V)를 포함하고, 상기 제2개폐 유닛(242)이 상기 제2연장관부(215) 및 제2공급관(310)의 단부를 각각 개폐하는 한쌍의 제2밸브(242V)를 포함한다. 따라서 상기 제1밸브(241V)의 개폐에 따라서 상기 제1연장관부(213) 및 제1공급관(110)이 연통/분리되고, 상기 제2밸브(242V)의 개폐에 따라서 상기 제2연장관부(215) 및 제2공급관(310)이 연통/분리될 것이다. 상기 나선관부(211)에 추출된 줄기세포 및 증식 배지가 공급되는 과정에서는, 상기 제1개폐 유닛(241)에 의하여 상기 제1연장관부(213) 및 제1공급관(110)은 연통되고, 상기 제2개폐 유닛(242)에 의하여 상기 제2연장관부(215) 및 제2공급관(310)은 분리될 것이다. 그리고 상기 나선관부(211)에 추출된 줄기세포 및 증식 배지가 공급이 완료되면, 상기 제1개폐 유닛(241)에 의하여 상기 제1연장관부(213) 및 제1공급관(110)은 분리될 것이다.The first opening and closing unit 241 selectively communicates / separates the first extension pipe part 213 and the first supply pipe 110. In addition, the second opening and closing unit 242 selectively communicates / separates the second extension pipe part 215 and the second supply pipe 310 to be described later. Substantially, the supply of the extracted stem cells and the proliferation medium through the first extension tube 213 and the first supply tube 110 by the first opening and closing unit 241 is selectively made. And the second opening and closing unit 242 is selectively made of the supply of proliferated stem cells through the second extension tube 215 and the second supply tube 310. In the present embodiment, the first opening and closing unit 241 includes a pair of first valves 241V for opening and closing the ends of the first extension pipe part 213 and the first supply pipe 110, respectively, and the second The opening and closing unit 242 includes a pair of second valves 242V for opening and closing ends of the second extension pipe part 215 and the second supply pipe 310, respectively. Accordingly, the first extension pipe part 213 and the first supply pipe 110 communicate / separate according to the opening and closing of the first valve 241V, and the second extension pipe part (2) according to the opening and closing of the second valve 242V. 215 and the second supply pipe 310 will be in communication / separation. In the process of supplying the stem cells and the proliferation medium extracted to the spiral tube portion 211, the first extension tube portion 213 and the first supply tube 110 is in communication by the first opening and closing unit 241, The second extension pipe part 215 and the second supply pipe 310 will be separated by the second opening and closing unit 242. When the stem cells and the proliferation medium extracted to the spiral tube part 211 are supplied, the first extension tube part 213 and the first supply tube 110 will be separated by the first opening / closing unit 241. .
한편 상기 회전 구동 유닛(250)은, 상기 증식관(210)을 회전시킨다. 실질적으로 본 실시예에서는, 상기 회전 구동 유닛(250)이 상기 증식관(210)이 그 내부에 설치되는 상기 증식 용기(220)를 회전시킨다. 물론, 상기 증식 용기(220)가 고정된 상태에서, 상기 회전 구동 유닛(250)이 상기 증식관(210)을 회전시켜도 무방하다. 다만, 상기 회전 구동 유닛(250)에 의한 상기 증식관(210)의 회전은, 상기 제1 및 제2개폐 유닛(241)(242)에 의하여 상기 제1 및 제2연장관부(213)(215)와 상기 제1 및 제2공급관(110)(310)이 분리된 상태에서, 이루어질 것이다.On the other hand, the rotation drive unit 250 rotates the growth tube 210. Substantially in this embodiment, the rotation drive unit 250 rotates the growth vessel 220 in which the growth tube 210 is installed therein. Of course, in the state in which the growth vessel 220 is fixed, the rotation drive unit 250 may rotate the growth tube 210. However, the rotation of the propagation pipe 210 by the rotation driving unit 250 is performed by the first and second opening and closing units 241 and 242 to the first and second extension pipe parts 213 and 215. ) And the first and second supply pipes 110 and 310 in a separated state.
그리고 상기 순환 펌프(260)는, 줄기세포 및 증식 배지가 상기 나선관부(211) 및 상기 순환관부(217)를 순환하도록 압송한다. 상술한 바와 같이, 실질적으로 상기 순환 펌프(260)는, 상기 나선관부(211)의 타단에서 일단으로 줄기세포와 증식 배지를 유동시킨다. 상기 순환 펌프(260)에 의한 상기 줄기세포 및 증식 배지의 압송은, 상기 제1 및 제2개폐 유닛(241)(242)에 의하여 상기 제1 및 제2연장관부(213)(215)와 상기 제1 및 제2공급관(110)(310)이 분리된 상태에서, 이루어질 것이다. The circulation pump 260 pumps the stem cells and the proliferation medium to circulate the spiral tube portion 211 and the circulation tube portion 217. As described above, the circulation pump 260 substantially flows the stem cells and the growth medium from one end of the spiral tube portion 211 to one end. The pumping of the stem cells and the proliferation medium by the circulation pump 260 is performed by the first and second opening and closing units 241 and 242 and the first and second extension pipe parts 213 and 215. In a state in which the first and second supply pipes 110 and 310 are separated, it will be made.
또한 상기 온도 조절 유닛(270)은, 상기 증식관(210)에서의 줄기세포의 증식을 위한 온도를 조절한다. 예를 들면, 상기 온도 조절 유닛(270)은, 상기 증식 용기(220)의 일측에 설치되어 상기 증식 용기(220)의 내부 온도를 기설정된 온도를 유지하도록 조절할 수 있다.In addition, the temperature control unit 270, adjusts the temperature for the proliferation of stem cells in the growth tube 210. For example, the temperature control unit 270 may be installed at one side of the growth vessel 220 to adjust the internal temperature of the growth vessel 220 to maintain a predetermined temperature.
상기 공기 공급 유닛(280)은 상기 증식관(210)으로 공기를 공급한다. 상기 공기 공급 유닛(280)은, 상기 증식관(210)의 일측, 예를 들면, 상기 나선관부(211)의 일측에 연결되어 상기 증식관(210)의 내부로 공기를 공급한다. 이때 상기 공기 공급 유닛(280)은, 대기에 비하여 상대적으로 이산화탄소의 농도가 증가된 공기를 상기 증식관(210)으로 공급한다. 또한 상기 공기 공급 유닛(280)에 의하여 상기 증식관(210)으로 공급되는 공기는, 필터(281)에 의하여 이물질 등이 제거된 상태로 공급될 수 있다.The air supply unit 280 supplies air to the growth tube 210. The air supply unit 280 is connected to one side of the growth tube 210, for example, one side of the spiral tube portion 211, and supplies air to the interior of the growth tube 210. In this case, the air supply unit 280 supplies air to the growth pipe 210 in which the concentration of carbon dioxide is increased relative to the atmosphere. In addition, the air supplied to the growth pipe 210 by the air supply unit 280 may be supplied in a state in which foreign matters and the like are removed by the filter 281.
한편 상기 원심 분리기(300)는, 상기 줄기세포 증식기(200)로부터 증식된 줄기세포를 공급받아서 성분별로 분리한다. 상기 원심 분리기(300)로는, 주지된 다양한 종류의 원심 분리기가 사용될 수 있다. 그리고 상기 원심 분리기(300)는, 제2공급관(310)을 포함한다. 상기 제2공급관(310)은, 상기 제2연장관부(215)와 연통되어 상기 줄기세포 증식기(200)로부터 증식된 줄기세포를 공급받는다.On the other hand, the centrifuge 300 receives the stem cells proliferated from the stem cell multiplier 200 and separates by component. As the centrifuge 300, various kinds of well-known centrifuges may be used. The centrifuge 300 includes a second supply pipe 310. The second supply pipe 310 is in communication with the second extension tube 215 receives the stem cells proliferated from the stem cell multiplier 200.
상기 제1공급 펌프(410)는, 상기 줄기세포 추출기(100)에 의하여 추출된 줄기세포 및 상기 증식 배지 공급 유닛(230)에 의하여 공급되는 증식 배지를 상기 증식관(210)으로 압송하고, 상기 제2공급 펌프(420)는, 상기 줄기세포 증식기(200)에 의하여 증식된 줄기세포를 상기 원심 분리기(300)로 압송한다. 실질적으로 상기 제1공급 펌프(410)에 의한 추출된 줄기세포 및 증식 배지의 압송과 상기 제2공급 펌프(420)에 의한 증식된 줄기세포의 압송은, 각각 상기 제1연장관부(213) 및 제1공급관(110)이 연통된 상태 또는 상기 제2연장관부(215) 및 제2공급관(310)이 연통된 상태에서, 이루어질 것이다.The first supply pump 410 pumps the stem cells extracted by the stem cell extractor 100 and the growth medium supplied by the growth medium supply unit 230 to the growth tube 210. The second supply pump 420 pumps the stem cells proliferated by the stem cell multiplier 200 to the centrifuge 300. Substantially, the pumping of the stem cells and the proliferation medium extracted by the first supply pump 410 and the pumping of the stem cells proliferated by the second supply pump 420 may be performed by the first extension pipe part 213 and, respectively. In a state in which the first supply pipe 110 is in communication, or in a state in which the second extension pipe part 215 and the second supply pipe 310 are in communication, it will be made.
이하에서는 본 발명의 실시예에 의한 줄기세포 연속 증식, 추출 및 분리 방법을 첨부된 도면을 참조하여 보다 상세하게 설명한다.Hereinafter, with reference to the accompanying drawings, the stem cell continuous proliferation, extraction and separation method according to an embodiment of the present invention will be described in more detail.
도 2는 본 발명의 제1실시예에 의한 줄기세포 연속 증식, 추출 및 분리 방법을 보인 플로우 챠트이다.Figure 2 is a flow chart showing a method of continuous proliferation, extraction and separation of stem cells according to the first embodiment of the present invention.
도 2를 참조하면, 본 실시예에 의한 줄기세포 연속 증식, 추출 및 분리 방법은, 줄기세포 추출 단계(S100), 추출 줄기세포 및 증식 배지 공급 단계(S200), 줄기세포 증식 단계(S300), 증식 줄기세포 공급 단계(S400) 및 줄기세포 분리 단계(S500)를 포함한다.Referring to Figure 2, the stem cell continuous proliferation, extraction and separation method according to the present embodiment, stem cell extraction step (S100), extracted stem cells and proliferation medium supply step (S200), stem cell proliferation step (S300), Proliferating stem cell supply step (S400) and stem cell separation step (S500).
보다 상세하게는, 상기 줄기세포 추출 단계(S100)에서는 줄기세포가, 줄기세포 추출기(100)에 의하여 시료로부터 추출된다. 상술한 바와 같이, 상기 줄기세포 추출기(100)에 의한 줄기세포의 추출은 주지의 기술을 사용할 수 있으므로, 이에 대한 상세한 설명은 생략하기로 한다.More specifically, in the stem cell extraction step (S100), stem cells are extracted from the sample by the stem cell extractor (100). As described above, the extraction of stem cells by the stem cell extractor 100 may use a well-known technique, and thus a detailed description thereof will be omitted.
다음으로, 상기 추출 줄기세포 및 증식 배지 공급 단계(S200)에서는, 상기 줄기세포 추출 단계(S100)에서 추출된 줄기세포 및 줄기세포의 증식을 위한 증식 배지가 줄기세포 증식기(200), 실질적으로 증식관(210)에 공급된다. 보다 상세하게는, 상기 줄기세포 추출기(100)에서 추출된 줄기세포 및 증식 배지 공급 유닛(230)에 의하여 공급되는 증식 배지가 제1공급 펌프(410)에 의하여 상기 증식관(210)으로 압송될 것이다. 추출된 줄기세포 및 증식 배지의 공급은, 제1연장관부(213) 및 제1공급관(110)은 제1개폐 유닛(241)에 의하여 연통되고, 제2연장관부(215) 및 제2공급관(310)은 제2개폐 유닛(242)에 의하여 분리된 상태에서, 상기 제1연장관부(213) 및 제1공급관(110)을 통하여 이루어질 것이다.Next, in the extracting stem cells and proliferation medium supply step (S200), the proliferation medium for proliferation of the stem cells and stem cells extracted in the stem cell extraction step (S100) is a stem cell multiplier 200, substantially proliferating Supplied to the tube 210. More specifically, the stem cells extracted from the stem cell extractor 100 and the growth medium supplied by the growth medium supply unit 230 may be pumped to the growth tube 210 by the first supply pump 410. will be. Supply of the extracted stem cells and proliferation medium, the first extension tube portion 213 and the first supply tube 110 is communicated by the first opening and closing unit 241, the second extension tube portion 215 and the second supply tube ( The 310 is separated by the second opening and closing unit 242, it will be made through the first extension pipe portion 213 and the first supply pipe 110.
그리고 상기 줄기세포 증식 단계(S300)에서는, 상기 추출 줄기세포 및 증식 배지 공급 단계(S200)에서 상기 증식관(210)에 공급된 줄기세포가 상기 증식관(210)의 내부에서 증식된다. 본 실시예에서는, 상기 줄기세포 증식 단계(S300)에서, 상기 증식관(210)의 회전 및 상기 증식관(210), 실질적으로 나선관부(211)에서의 줄기세포 및 증식 배지의 순환이 이루어진다. And in the stem cell growth step (S300), the stem cells supplied to the growth tube 210 in the extracting stem cells and growth medium supply step (S200) is proliferated inside the growth tube 210. In this embodiment, in the stem cell proliferation step (S300), rotation of the growth tube 210 and circulation of the stem cells and the growth medium in the growth tube 210, substantially the spiral tube portion 211 is made.
보다 상세하게는, 상기 줄기세포 증식 단계(S300)에서는, 상기 제1 및 제2연장관부(213)(215)와 상기 제1 및 제2공급관(110)(310)이 상기 제1 및 제2개폐 유닛(241)(242)에 의하여 분리된 상태에서, 상기 회전 구동 유닛(250)이 상기 증식관(210)을 회전시킨다. 그리고 상기 줄기세포 증식 단계(S300)에서는, 상기 제1 및 제2연장관부(213)(215)와 상기 제1 및 제2공급관(110)(310)이 상기 제1 및 제2개폐 유닛(241)(242)에 의하여 분리된 상태에서, 순환 펌프(260)가, 줄기세포 및 증식 배지가 상기 증식관(210)을 순환하도록 압송한다.More specifically, in the stem cell proliferation step (S300), the first and second extension tube portion 213, 215 and the first and second supply tube 110, 310 is the first and second In the state separated by the opening / closing unit 241 or 242, the rotation driving unit 250 rotates the growth tube 210. In the stem cell proliferation step (S300), the first and second extension pipe parts 213 and 215 and the first and second supply pipes 110 and 310 are connected to the first and second opening and closing units 241. In a state separated by 242), the circulation pump 260 pumps the stem cells and the growth medium to circulate the growth pipe 210.
또한 상기 줄기세포 증식 단계(S300)에서는, 온도 조절 유닛(270)에 의한 온도 조절 및 공기 공급 유닛(280)에 의한 공기의 공급도 이루어진다. 즉 상기 줄기세포 증식 단계(S300)에서는, 상기 온도 조절 유닛(270)이, 상기 증식관(210)에서의 줄기세포의 증식을 위한 온도를 조절하고, 상기 공기 공급 유닛(280)이, 상기 증식관(210)의 내부로 공기를 공급한다.In addition, the stem cell growth step (S300), the temperature control by the temperature control unit 270 and the air supply by the air supply unit 280 is also made. That is, in the stem cell growth step (S300), the temperature control unit 270, the temperature for the growth of stem cells in the growth tube 210, the air supply unit 280, the growth Air is supplied to the inside of the pipe (210).
마지막으로, 상기 증식 줄기세포 공급 단계(S400) 및 줄기세포 분리 단계(S500)에서는, 증식된 줄기세포의 원심 분리기(300)로의 공급과, 상기 원심 분리기(300)에서의 줄기세포의 성분별 분리가 이루어진다. 상기 증식 줄기세포 공급 단계(S400)에서는, 상기 제1연장관부(213) 및 제1공급관(110)은 제1개폐 유닛(241)에 의하여 분리되고, 상기 제2연장관부(215) 및 제2공급관(310)은 상기 제2개폐 유닛(242)에 의하여 분리된 상태에서, 상기 제2연장관부(215) 및 제2공급관(310)을 통하여 증식된 줄기세포가 상기 줄기세포 증식기(200)로부터 상기 원심 분리기(300)로 공급될 것이다. 또한 상기 줄기세포 분리 단계(S500)에서는, 상기 제2연장관부(215) 및 제2공급관(310)이 상기 제2개폐 유닛(242)에 의하여 분리된 상태에서, 상기 원심 분리기(300)에 의하여 줄기세포가 성분별로 분리될 것이다.Finally, in the proliferating stem cell supplying step (S400) and the stem cell separation step (S500), supply of the proliferated stem cells to the centrifuge 300, and separation of the components of the stem cells in the centrifuge 300 Is done. In the proliferating stem cell supplying step (S400), the first extension pipe part 213 and the first supply pipe 110 are separated by a first opening and closing unit 241, the second extension pipe part 215 and the second. In the state in which the supply pipe 310 is separated by the second opening and closing unit 242, the stem cells proliferated through the second extension pipe part 215 and the second supply pipe 310 are separated from the stem cell multiplier 200. It will be supplied to the centrifuge 300. In addition, in the stem cell separation step (S500), in the state in which the second extension tube part 215 and the second supply tube 310 are separated by the second opening and closing unit 242, by the centrifuge 300 Stem cells will be separated by component.
특히, 본 실시예에서는, 상기 줄기세포 추출기(100)에 의한 줄기세포의 추출, 상기 줄기세포 증식기(200)에 의한 줄기세포의 증식 및 상기 원심 분리기(300)에 의한 줄기세포의 성분별 분리가 연속적으로 이루어진다. 이를 위하여 상기 추출 줄기세포 및 증식 배지 공급 단계(S200)가 완료되면, 상기 줄기세포 증식 단계(S300)의 완료와 무관하게 상기 줄기세포 추출 단계(S100)가 개시된다. 또한 상기 증식 줄기세포 공급 단계(S400)가 완료되면, 상기 줄기세포 분리 단계(S500)의 완료와 무관하게 상기 추출 줄기세포 및 증식 배지 공급 단계(S200)가 개시된다. 따라서 본 실시예에서는, 연속적인 줄기세포의 증식, 추출 및 성분별 분리가 이루어질 수 있다.In particular, in the present embodiment, the stem cell extraction by the stem cell extractor 100, the proliferation of stem cells by the stem cell multiplier 200 and the separation of the stem cells by component centrifuge 300 It is done continuously. To this end, when the extraction of the stem cells and the growth medium supply step (S200) is completed, the stem cell extraction step (S100) is initiated regardless of the completion of the stem cell growth step (S300). In addition, when the proliferating stem cell supply step (S400) is completed, regardless of the completion of the stem cell separation step (S500), the extracted stem cells and proliferation medium supply step (S200) is started. Therefore, in this embodiment, continuous stem cells can be proliferated, extracted, and separated by components.
본 발명의 권리범위는 위에서 설명된 실시예에 한정되지 않고 특허청구범위에 기재된 바에 의해 정의되며, 본 발명의 기술분야에서 통상의 지식을 가진 자가 특허청구범위에 기재된 권리범위 내에서 다양한 변형과 개작을 할 수 있다는 것은 자명하다.The scope of the present invention is not limited to the embodiments described above, but is defined by the claims, and various modifications and adaptations within the scope of the claims are made by those skilled in the art. It is self evident.
이하에서는 본 발명의 제2실시예에 의한 줄기세포 연속 증식, 추출 및 분리 시스템을 첨부된 도면을 참조하여 보다 상세하게 설명한다.Hereinafter, with reference to the accompanying drawings, the stem cell continuous proliferation, extraction and separation system according to a second embodiment of the present invention will be described in more detail.
도 3은 본 발명의 제2실시예에 의한 줄기세포 연속 증식, 추출 및 분리 시스템을 개략적으로 보인 구성도이다. 본 실시예의 구성 요소 중 상술한 본 발명의 제1실시예의 구성 요소와 동일한 구성 요소에 대해서는, 도 1의 도면 부호를 원용하고, 이에 대한 상세한 설명을 생략하기로 한다.Figure 3 is a schematic view showing a stem cell continuous proliferation, extraction and separation system according to a second embodiment of the present invention. For the same components as those of the first embodiment of the present invention described above among the components of the present embodiment, reference numerals of FIG. 1 are used, and detailed description thereof will be omitted.
도 3을 참조하면, 본 실시예에 의한 줄기세포 연속 증식, 추출 및 분리 시스템(2)은, 틸팅 유닛(290)을 더 포함한다. 상기 틸팅 유닛(290)은, 증식관(210), 실질적으로 상기 증식관(210)및 증식 용기(220)를 기설정된 틸팅 각도로 틸팅한다. 다만, 본 실시예에서도, '틸팅 각도'는, 나선관부(211)가, 수평면에 대하여 0° 초과 및 45°이하의 각도로 경사질 수 있는 범위에서 설정될 것이다.Referring to FIG. 3, the stem cell continuous proliferation, extraction and separation system 2 according to the present embodiment further includes a tilting unit 290. The tilting unit 290 tilts the growth tube 210, substantially the growth tube 210 and the growth vessel 220 at a predetermined tilting angle. However, in the present embodiment, the 'tilting angle' will be set in a range in which the spiral tube portion 211 can be inclined at an angle of more than 0 ° and 45 ° or less with respect to the horizontal plane.

Claims (21)

  1. 나선 형상으로 형성되는 나선관부(211), 상기 나선관부(211)의 일단에서 연장되어 외부로부터 추출된 줄기세포 및 줄기세포의 증식을 위한 증식 배지를 공급받는 제1연장관부(213) 및 상기 나선관부(211)의 타단에서 연장되어 외부로 증식된 줄기세포를 공급하는 제2연장관부(215)를 포함하고, 줄기세포의 증식이 이루어지는 증식관(210); The spiral tube portion 211 formed in a spiral shape, the first extension tube portion 213 and the spiral extending from one end of the spiral tube portion 211 and receiving a growth medium for proliferation of stem cells and stem cells extracted from the outside A proliferation tube 210 including a second extension tube portion 215 extending from the other end of the tube portion 211 to supply stem cells proliferated to the outside, and proliferating the stem cells;
    상기 증식관(210)에 증식 배지를 공급하는 증식 배지 공급 유닛(230); A growth medium supply unit 230 for supplying a growth medium to the growth pipe 210;
    상기 제1연장관부(213)를 상기 증식관(210)으로 추출된 줄기세포를 공급하기 위한 제1공급관(110)과 선택적으로 연통/분리시키는 제1개폐 유닛(241); A first opening / closing unit 241 for selectively communicating / separating the first extension tube part 213 with the first supply tube 110 for supplying the stem cells extracted into the growth tube 210;
    상기 제2연장관부(215)를 상기 증식관(210)에서 증식된 줄기세포를 공급하기 위한 제2공급관(310)과 선택적으로 연통/분리시키는 제2개폐 유닛(242); 및 A second opening and closing unit 242 for selectively communicating / separating the second extension tube part 215 with a second supply tube 310 for supplying stem cells proliferated in the growth tube 210; And
    상기 증식관(210)을 회전시키는 회전 구동 유닛(250); 을 포함하고, A rotation drive unit 250 for rotating the growth tube 210; Including,
    상기 나선관부(211)에 추출된 줄기세포 및 증식 배지가 공급되면, 상기 제1 및 제2개폐 유닛(241)(242)에 의하여 상기 제1 및 제2연장관부(213)(215)와 상기 제1 및 제2공급관(110)(310)이 분리된 상태에서, 상기 회전 구동 유닛(250)에 의하여 상기 증식관(210)이 회전하는 줄기세포 증식기.When the stem cells and the proliferation medium extracted to the spiral tube part 211 are supplied, the first and second extension tube parts 213 and 215 and the first and second opening and closing units 241 and 242 are supplied. Stem cell multiplier in which the growth pipe 210 is rotated by the rotation drive unit 250 in a state in which the first and second supply pipes (110) (310) are separated.
  2. 제 1 항에 있어서, The method of claim 1,
    상기 증식관(210)은, 상기 제1연장관부(213)에 인접하는 상기 나선관부(211)의 일측 또는 상기 제1연장관부(213)의 일측에 일단이 연결되고, 상기 제2연장관부(215)에 인접하는 상기 나선관부(211)의 다른 일측 또는 상기 제2연장관부(215)의 일측에 타단이 연결되는 순환관부(217)를 더 포함하고, The growth tube 210 is connected to one side of the spiral tube portion 211 adjacent to the first extension tube portion 213 or one side of the first extension tube portion 213, and the second extension tube portion ( Further comprising a circulation pipe portion 217 that the other end is connected to the other side of the spiral tube portion 211 adjacent to the 215 or one side of the second extension tube portion 215,
    상기 제1 및 제2개폐 유닛(241)(242)에 의하여 상기 제1 및 제2연장관부(213)(215)와 상기 제1 및 제2공급관(110)(310)이 분리된 상태에서, 줄기세포 및 증식 배지가 상기 나선관부(211)를 순환하도록 상기 나선관부(211)의 타단에서 일단으로 줄기세포 및 증식 배지를 압송하는 순환 펌프(260)를 더 포함하는 줄기세포 증식기.In a state where the first and second extension pipe parts 213 and 215 and the first and second supply pipes 110 and 310 are separated by the first and second opening and closing units 241 and 242, And a circulation pump (260) for pumping stem cells and proliferation medium from one end of the helix tube portion (211) to one end so that the stem cells and the growth medium circulate through the helix tube portion (211).
  3. 제 2 항에 있어서, The method of claim 2,
    적어도 상기 나선관부(211)는, 수평면에 대하여 0° 초과 및 45°이하의 각도로 경사지게 배치되는 줄기세포 증식기.At least the spiral tube portion 211, the stem cell multiplier is disposed inclined at an angle of more than 0 ° and less than 45 ° with respect to the horizontal plane.
  4. 제 1 항에 있어서, The method of claim 1,
    상기 증식관(210)이 그 내부에 배치되고, 상기 회전 구동 유닛(250)에 의하여 상기 증식관(210)과 함께 회전하는 증식 용기(220)를 더 포함하는 줄기세포 증식기.The propagation tube 210 is disposed therein, further comprising a growth vessel 220 which rotates together with the growth tube 210 by the rotation drive unit 250 further stem cell proliferator.
  5. 제 1 항에 있어서, The method of claim 1,
    상기 증식관(210)에서의 줄기세포의 증식을 위한 온도를 조절하는 온도 조절 유닛(270)을 더 포함하는 줄기세포 증식기.Stem cell multiplier further comprises a temperature control unit for adjusting the temperature for the proliferation of stem cells in the growth tube 210.
  6. 제 1 항에 있어서, The method of claim 1,
    상기 증식관(210)으로 공기를 공급하는 공기 공급 유닛(280)을 더 포함하는 줄기세포 증식기.Stem cell multiplier further comprises an air supply unit (280) for supplying air to the growth pipe (210).
  7. 제 1 항의 줄기세포 증식기를 사용한 줄기세포 증식 방법에 있어서: In the stem cell proliferation method using the stem cell multiplier of claim 1:
    추출된 줄기세포 및 줄기세포의 증식을 위한 증식 배지가 상기 증식관(210)에 공급되는 추출 줄기세포 및 증식 배지 공급 단계(S200); 및 A step of supplying the extracted stem cells and the propagation medium, wherein the proliferation medium for proliferation of the extracted stem cells and stem cells is supplied to the growth tube 210; And
    줄기세포가 상기 증식관(210)의 내부에서 증식되는 줄기세포 증식 단계(S300); 를 포함하는 줄기세포 증식 방법.Stem cell proliferation step of proliferating inside the growth tube 210, the stem cell (S300); Stem cell proliferation method comprising a.
  8. 제 7 항에 있어서, The method of claim 7, wherein
    상기 추출 줄기세포 및 증식 배지 공급 단계(S200)에서, In the extracting stem cells and proliferation medium supply step (S200),
    상기 제1개폐 유닛(241)에 의하여 상기 제1연장관부(213) 및 제1공급관(110)이 연통되고, 상기 제2개폐 유닛(242)에 의하여 상기 제2연장관부(215) 및 제2공급관(310)이 분리된 상태에서 상기 제1연장관부(213) 및 제1공급관(110)을 통하여 추출된 줄기세포 및 증식 배지가 상기 증식관(210)으로 공급되는 줄기세포 증식 방법.The first extension pipe part 213 and the first supply pipe 110 communicate with each other by the first opening / closing unit 241, and the second extension pipe part 215 and the second opening by the second opening / closing unit 242. Stem cell proliferation method in which the stem cells and the proliferation medium extracted through the first extension tube portion 213 and the first supply tube 110 in a state in which the supply tube 310 is separated are supplied to the growth tube 210.
  9. 제 7 항에 있어서, The method of claim 7, wherein
    상기 줄기세포 증식 단계(S300)에서, In the stem cell proliferation step (S300),
    상기 회전 구동 유닛(250)이, 상기 제1 및 제2개폐 유닛(241)(242)에 의하여 상기 제1 및 제2연장관부(213)(215)와 상기 제1 및 제2공급관(110)(310)이 분리된 상태에서, 상기 증식관(210)을 회전시키는 줄기세포 증식 방법.The first and second extension pipe parts 213 and 215 and the first and second supply pipes 110 are rotated by the rotation driving unit 250 by the first and second opening and closing units 241 and 242. Stem cell proliferation method for rotating the growth tube 210 in the state 310 is separated.
  10. 제 7 항에 있어서, The method of claim 7, wherein
    상기 줄기세포 증식 단계(S300)에서, In the stem cell proliferation step (S300),
    순환 펌프(260)가, 줄기세포 및 증식 배지가 상기 증식관(210)을 순환하도록 압송하는 줄기세포 증식 방법.Stem cell proliferation method, the circulation pump 260, the stem cells and proliferation medium is pumped to circulate the growth pipe (210).
  11. 제 7 항에 있어서, The method of claim 7, wherein
    상기 줄기세포 증식 단계(S300)에서, In the stem cell proliferation step (S300),
    온도 조절 유닛(270)이, 상기 증식관(210)에서의 줄기세포의 증식을 위한 온도를 조절하고, Temperature control unit 270, to adjust the temperature for the proliferation of stem cells in the growth tube 210,
    공기 공급 유닛(280)이, 상기 증식관(210)의 내부로 공기를 공급하는 줄기세포 증식 방법.Stem cell proliferation method that the air supply unit 280, supplying air into the growth tube 210.
  12. 시료로부터 줄기세포를 추출하는 줄기세포 추출기(100); A stem cell extractor 100 for extracting stem cells from a sample;
    상기 줄기세포 추출기(100)로부터 추출된 줄기세포를 공급받아서 증식하는 제 1 항 내지 제 6 항 중 어느 한 항의 줄기세포 증식기(200); 및 A stem cell multiplier according to any one of claims 1 to 6 for receiving and proliferating the stem cells extracted from the stem cell extractor 100; And
    상기 줄기세포 증식기(200)로부터 증식된 줄기세포를 공급받아서 성분별로 분리하는 원심 분리기(300); 를 포함하는 줄기세포 연속 증식, 추출 및 분리 시스템.A centrifugal separator 300 for receiving the stem cells proliferated from the stem cell multiplier 200 and separating the stem cells by components; Stem cell continuous proliferation, extraction and separation system comprising a.
  13. 제 12 항에 있어서, The method of claim 12,
    상기 줄기세포 추출기(100)는, 상기 제1연장관부(213)와 연통되어 추출된 줄기세포를 상기 줄기세포 증식기(200)에 공급하는 제1공급관(110)을 포함하고, The stem cell extractor 100 includes a first supply pipe 110 for supplying the stem cells extracted in communication with the first extension tube part 213 to the stem cell multiplier 200,
    상기 원심 분리기(300)는, 상기 제2연장관부(215)와 연통되어 증식된 줄기세포를 상기 줄기세포 증식기(200)로부터 공급받는 제2공급관(310)을 포함하는 줄기세포 연속 증식, 추출 및 분리 시스템.The centrifuge 300, the stem cell continuous proliferation, extraction and the stem cell comprising a second supply pipe 310 receives the stem cells proliferated in communication with the second extension tube portion 215 from the stem cell multiplier 200 Separation system.
  14. 제 13 항에 있어서, The method of claim 13,
    상기 제1개폐 유닛(241)에 의하여 상기 제1연장관부(213) 및 제1공급관(110)이 연통된 상태에서, 상기 줄기세포 추출기(100)에 의하여 추출된 줄기세포 및 상기 증식 배지 공급 유닛(230)에 의하여 공급되는 증식 배지를 상기 증식관(210)으로 압송하는 제1공급 펌프(410)를 더 포함하는 줄기세포 연속 증식, 추출 및 분리 시스템.Stem cells and the proliferation medium supply unit extracted by the stem cell extractor 100 in a state where the first extension pipe part 213 and the first supply pipe 110 communicate with each other by the first opening and closing unit 241. Stem cell continuous proliferation, extraction and separation system further comprises a first supply pump 410 for pumping the growth medium supplied by 230 to the growth tube 210.
  15. 제 13 항에 있어서, The method of claim 13,
    상기 제2개폐 유닛(242)에 의하여 상기 제2연장관부(215) 및 제2공급관(310)이 연통된 상태에서, 상기 줄기세포 증식기(200)에 의하여 증식된 줄기세포를 상기 원심 분리기(300)로 압송하는 제2공급 펌프(420)를 더 포함하는 줄기세포 연속 증식, 추출 및 분리 시스템.In the state in which the second extension pipe part 215 and the second supply pipe 310 are in communication with each other by the second opening and closing unit 242, the stem cells proliferated by the stem cell multiplier 200 are centrifugal separator 300. Stem cell continuous proliferation, extraction and separation system further comprises a second feed pump (420) for pumping.
  16. 제 12 항의 줄기세포 연속 증식, 추출 및 분리 시스템을 사용하여 줄기세포를 연속 증식, 추출 및 분리하는 방법에 있어서: 13. A method of continuously proliferating, extracting and isolating stem cells using the stem cell continuous proliferation, extraction and isolation system of claim 12:
    줄기세포가, 상기 줄기세포 추출기(100)에 의하여 추출되는 줄기세포 추출 단계(S100); Stem cells, the stem cell extraction step is extracted by the stem cell extractor (100) (S100);
    상기 줄기세포 추출 단계(S100)에서 추출된 줄기세포 및 줄기세포의 증식을 위한 증식 배지가 상기 증식관(210)에 공급되는 추출 줄기세포 및 증식 배지 공급 단계(S200); Extracting stem cells and proliferation medium supplied with the proliferation medium for proliferation of the stem cells and the stem cells extracted in the stem cell extraction step (S100) to the proliferation tube 210 (S200);
    상기 추출 줄기세포 및 증식 배지 공급 단계(S200)에서 상기 증식관(210)에 공급된 줄기세포가 상기 증식관(210)의 내부에서 증식되는 줄기세포 증식 단계(S300);A stem cell proliferation step of proliferating the stem cells supplied to the proliferation tube 210 in the extracting stem cells and the proliferation medium supplying step (S200) inside the proliferation tube 210 (S300);
    상기 줄기세포 증식 단계(S300)에서 증식된 줄기세포가 상기 원심 분리기(300)에 공급되는 증식 줄기세포 공급 단계(S400); 및 Proliferating stem cell supply step (S400) is the stem cells proliferated in the stem cell proliferation step (S300) is supplied to the centrifuge (300); And
    상기 증식 줄기세포 공급 단계(S400)에서 공급된 줄기세포가 상기 원심 분리기(300)에 의하여 성분별로 분리되는 줄기세포 분리 단계(S500); 를 포함하는 줄기세포 연속 증식, 추출 및 분리 방법.A stem cell separation step of separating the stem cells supplied by the proliferating stem cell supplying step (S400) for each component by the centrifuge 300 (S500); Stem cell continuous proliferation, extraction and separation method comprising a.
  17. 제 16 항에 있어서, The method of claim 16,
    상기 추출 줄기세포 및 증식 배지 공급 단계(S200)에서, In the extracting stem cells and proliferation medium supply step (S200),
    상기 제1개폐 유닛(241)에 의하여 상기 제1연장관부(213) 및 제1공급관(110)이 연통되고, 상기 제2개폐 유닛(242)에 의하여 상기 제2연장관부(215) 및 제2공급관(310)이 분리된 상태에서, 상기 제1연장관부(213) 및 제1공급관(110)을 통하여 상기 줄기세포 추출기(100)에서 추출된 줄기세포 및 상기 증식 배지 공급 유닛(230)으로부터의 증식 배지가 상기 증식관(210)으로 공급되는 줄기세포 연속 증식, 추출 및 분리 방법.The first extension pipe part 213 and the first supply pipe 110 communicate with each other by the first opening / closing unit 241, and the second extension pipe part 215 and the second opening by the second opening / closing unit 242. In a state in which the supply pipe 310 is separated, the stem cells extracted from the stem cell extractor 100 through the first extension pipe part 213 and the first supply pipe 110 and from the growth medium supply unit 230. Stem cell continuous proliferation, extraction and separation method, the growth medium is supplied to the growth tube (210).
  18. 제 16 항에 있어서, The method of claim 16,
    상기 줄기세포 증식 단계(S300)에서, In the stem cell proliferation step (S300),
    상기 회전 구동 유닛(250)이, 상기 제1 및 제2개폐 유닛(241)(242)에 의하여 상기 제1 및 제2연장관부(213)(215)와 상기 제1 및 제2공급관(110)(310)이 분리된 상태에서, 상기 증식관(210)을 회전시키는 줄기세포 연속 증식, 추출 및 분리 방법.The first and second extension pipe parts 213 and 215 and the first and second supply pipes 110 are rotated by the rotation driving unit 250 by the first and second opening and closing units 241 and 242. Stem cell continuous proliferation, extraction and separation method for rotating the growth tube 210 in a separated state (310).
  19. 제 16 항에 있어서, The method of claim 16,
    상기 줄기세포 증식 단계(S300)에서, In the stem cell proliferation step (S300),
    순환 펌프(260)가, 상기 제1 및 제2개폐 유닛(241)(242)에 의하여 상기 제1 및 제2연장관부(213)(215)와 상기 제1 및 제2공급관(110)(310)이 분리된 상태에서, 줄기세포 및 증식 배지가 상기 증식관(210)을 순환하도록 압송하는 줄기세포 연속 증식, 추출 및 분리 방법.The circulation pump 260 is connected to the first and second extension pipe parts 213 and 215 and the first and second supply pipes 110 and 310 by the first and second opening and closing units 241 and 242. Stem cell continuous proliferation, extraction and separation method in which the stem cells and proliferation medium are transported to circulate the growth tube 210 in the isolated state.
  20. 제 16 항에 있어서, The method of claim 16,
    상기 추출 줄기세포 및 증식 배지 공급 단계(S200)가 완료되면, 상기 줄기세포 증식 단계(S300)의 완료와 무관하게 상기 줄기세포 추출 단계(S100)가 개시되는 연속 증식, 추출 및 분리 방법.When the extraction of the stem cell and growth medium supply step (S200) is completed, regardless of the completion of the stem cell growth step (S300), the stem cell extraction step (S100) is a continuous proliferation, extraction and separation method.
  21. 제 16 항에 있어서, The method of claim 16,
    상기 증식 줄기세포 공급 단계(S400)가 완료되면, 상기 줄기세포 분리 단계(S500)의 완료와 무관하게 상기 추출 줄기세포 및 증식 배지 공급 단계(S200)가 개시되는 연속 증식, 추출 및 분리 방법.When the proliferating stem cell supply step (S400) is completed, regardless of the completion of the stem cell separation step (S500), the extraction of the stem cells and proliferation medium supply step (S200) is a continuous proliferation, extraction and separation method.
PCT/KR2018/006931 2018-06-20 2018-06-20 Device and method for proliferating stem cell, and system and method for continuously proliferating, extracting, and separating stem cell WO2019245070A1 (en)

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