WO2019237409A1 - 凝胶制备用自动化装置及使用方法 - Google Patents

凝胶制备用自动化装置及使用方法 Download PDF

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Publication number
WO2019237409A1
WO2019237409A1 PCT/CN2018/092086 CN2018092086W WO2019237409A1 WO 2019237409 A1 WO2019237409 A1 WO 2019237409A1 CN 2018092086 W CN2018092086 W CN 2018092086W WO 2019237409 A1 WO2019237409 A1 WO 2019237409A1
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WIPO (PCT)
Prior art keywords
gel
bottle
mold
automatic
plate
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PCT/CN2018/092086
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English (en)
French (fr)
Inventor
霍景瑞
王蕾
吴楠
张晶晶
刘颖
杨晓晖
段峰
邢鹏
田毅
刘英富
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刘英富
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Application filed by 刘英富 filed Critical 刘英富
Priority to KR1020207036992A priority Critical patent/KR20210003291A/ko
Publication of WO2019237409A1 publication Critical patent/WO2019237409A1/zh

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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44704—Details; Accessories
    • G01N27/44747—Composition of gel or of carrier mixture
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01K—MEASURING TEMPERATURE; MEASURING QUANTITY OF HEAT; THERMALLY-SENSITIVE ELEMENTS NOT OTHERWISE PROVIDED FOR
    • G01K1/00—Details of thermometers not specially adapted for particular types of thermometer
    • G01K1/14—Supports; Fastening devices; Arrangements for mounting thermometers in particular locations

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  • the invention belongs to the field of gel production, and relates to an automatic device for gel preparation and a use method.
  • Agarose nucleic acid gel electrophoresis is a common technique for separating, identifying, and purifying nucleic acid molecules. It is an indispensable and important analytical method in the research of nucleic acids. It is widely used in basic theoretical research, agricultural science, medical health, industrial production, national defense research, and forensic medicine. And commodity inspection and other fields, play an important role in the teaching and research of biochemistry and molecular biology.
  • Agarose nucleic acid gel electrophoresis can migrate negatively charged nucleic acid molecules to the positive electrode during electrophoresis.
  • agarose gel needs to be used as the electrophoresis support medium to play the role of molecular sieve, so that the migration of nucleic acid molecules of different sizes and conformations There are large differences in the rates, so as to achieve the purpose of separation, so the quality of the agarose gel determines the success of the entire gel electrophoresis experiment, which is the basis of nucleic acid research.
  • the basic procedures of traditional agarose gel production mainly include: selecting the mold, configuring the glue solution, the sol, adding the nucleic acid stain, pouring the gel, and leaving it to solidify.
  • the principle and method of the production are simple, it still exists in the operation process.
  • Some problems need to be overcome and resolved: (1) The nucleic acid stain added during the operation is toxic, and the long-term exposure of the nucleic acid dye to the experimenter has a high risk of carcinogenesis, which seriously affects the health of the experimenter and pollutes the environment; (2) Due to the amount of gel powder added in the actual process, the amount of liquid, and the temperature control, there are artificial subjective factors. The lack of standardization also causes experimental electrophoresis images to appear different and affect data analysis. (3) The entire production process It is manual operation, which is time-consuming, labor-intensive, and human error often affects the overall progress of the experiment.
  • the problem to be solved by the present invention is to provide an automatic device for gel preparation, which avoids contact between humans and reagents, improves the safety system, and simultaneously realizes automatic control, saves manpower, reduces labor intensity, saves gel preparation time, and improves Work efficiency, easy to use and reliable.
  • an automatic device for preparing a gel includes a gel bottle, and an upper end of the gel bottle is provided with a liquid inlet pipe corresponding to an inlet of the gel bottle and A dye gun, the gel bottle can be moved up, down, left and right, the gel bottle can be rotated along a horizontal axis perpendicular to and intersecting with its own axis, and a heating plate and a gel mold are provided at the lower end of the gel bottle.
  • a plurality of heat sinks are provided at the middle and lower portions of the outer ring of the gel bottle, and a temperature sensor is provided on the outer ring of the gel bottle, and the temperature sensor is electrically connected to the control center.
  • the gel bottle includes an upper inlet section and a lower fusion section, the inlet section is a trumpet opening upward, and the liquid inlet tube and the outlet end of the dye gun are provided on the inlet section.
  • the fusion section has a conical shape with a gradually increasing diameter, and the lower end of the fusion section is provided with rounded corners.
  • the middle and upper part of the gel bottle is fixed on a clamping frame
  • the clamping frame is fixed on the output shaft end of a rotating motor
  • the rotating motor is provided on a moving frame and is up and down relative to the moving frame Slide connection.
  • a lifting motor is provided on a side of the moving frame remote from the rotating motor, and the lifting motor drives the rotating motor to slide up and down with the moving frame through a wire screw mechanism.
  • the lower end of the lifting frame The connection plate is slidably connected to the support column left and right, and one side of the support column is provided with a moving motor, and the moving motor drives the connection plate to move through a wire-female screw structure.
  • the gel mold has a trough-shaped structure, and a plurality of partition plates are provided inside the gel mold to divide the interior of the gel mold into a plurality of independent spaces, one end of an upper end surface of each partition plate.
  • Each is provided with a diversion port, and the order of setting the multiple diversion ports is the order that the gel passes through each independent space in turn.
  • a semiconductor cooling plate is arranged at the lower end of the gel mold, and the semiconductor cooling plate and the control center Electrically connected, a radio frequency identification module is provided on the side of the gel mold, and the radio frequency identification module is electrically connected to the control center.
  • a fixing plate is provided at the lower end of the gel mold, and a pushing member is provided on one side of the fixing plate, and the output shaft of the pushing member is fixedly connected to the fixing plate.
  • a positioning column is provided on the side, the gel mold is arranged in the positioning column symmetrically arranged, and a guide column is provided on both sides of the fixed plate, and the axis of the guide column is arranged in parallel with the axis of the pushing member.
  • the dye gun is an injection needle structure
  • the dye gun includes a needle, a needle tube, and a piston
  • an outlet end of the needle is provided at an upper end of a gel bottle mouth
  • the piston is threadedly matched with a driven gear
  • the driven gear is meshed with a driving gear
  • the driving gear is rotated by a driving motor
  • the needle tube is fixedly connected to a fixed part of the device.
  • liquid inlet pipe is communicated with a liquid storage bottle
  • the liquid storage bottle is arranged in a bottle rack
  • an external liquid supplementation quantitative device is provided outside the liquid inlet tube
  • the automatic liquid supplementation quantitative device is connected with a control center
  • a peristaltic pump is arranged between the liquid inlet pipe and the storage bottle.
  • the method of using an automated device for gel preparation is as follows:
  • the first step determine the gel ratio and size, weigh a certain amount of gel powder and add it to the gel bottle, the control center starts, and the automatic rehydration quantifier automatically adds liquid according to the size and concentration of the gel;
  • Step 2 Grasp the gel bottle left or right or shake it by the holder, and transfer the gel bottle to the hot plate.
  • the gel bottle is quickly heated to the temperature required for the gel to be dispensed.
  • the third step through the synergistic effect of the semiconductor refrigeration chip and the external exhaust fan, the heated gel solution is rapidly cooled to the appropriate temperature required for the later dyes or other substances, and the exhausted gas is adsorbed and filtered through the adsorption layer to reduce the toxic substances. Pollution of the surrounding environment;
  • the fourth step start the dye gun, add dyes or other reagents, drive the piston to move through the drive motor, or use the stepper motor to drive the piston to complete the effect of adding reagents, or use a microsampler to complete the reagent addition;
  • Step 5 Place the gel mold on the fixed plate, the semiconductor cooling sheet at the bottom pre-cools the gel mold, and alarm buzzer at the same time, reminding whether the gel plate is properly placed and positioned;
  • Step 6 Start the rotary motor. After mixing the contents of the gel bottle, move the gel bottle to the top of the gel mold and pour it into the gel mold.
  • Step 7 After the cooling gel is coagulated, the gel mold is ejected under the action of the pusher, and the power of the device is turned off.
  • the present invention has the advantages and positive effects: 1.
  • the entire device is automatically performed according to the set parameters, reducing manual operation steps, the gel preparation is more standardized, and the success rate of gel production is greatly improved.
  • the gelation process only requires the weighing of artificial gel powder, and the other steps are completely completed by the instrument. There is no need for personnel to wait for operation. This reduces the contact of toxic substances and reduces the risk of accidental injury to the operator.
  • the structure is simple to use, highly automated and intelligent. Improve the efficiency of gel preparation; 2.
  • a dye gun which can realize the automatic addition of dyes or other reagents, reduce the long-term exposure of the nucleic acid staining agent to the experimenter, reduce the risk of carcinogenesis, prevent the health of the experimenter, prevent pollution to the environment, and improve the safety of use It is convenient and convenient, and it uses a driving motor to automatically feed material through the control center, saving manpower and improving work efficiency; 3. It is equipped with a rotary motor, a lifting motor and a mobile motor to realize the movement, rotation, and rotation of the gel bottle to ensure heating , Shake and pour glue function, high degree of automation, reduced Labor intensity and work efficiency are improved at the same time; 4.
  • a temperature sensor is set on the outer ring of the gel bottle, and a dynamic liquid supplement quantifier is set on the outside of the storage bottle.
  • the temperature control and the amount of liquid added are controlled by the control center.
  • Set up exhaust fan and adsorption layer When toxic gas is accidentally leaked, it can be processed and adsorbed in time to ensure the safety of the operator, while protecting the environment, stronger environmental protection and higher safety factor.
  • FIG. 1 is a schematic structural view of a front view of an automatic device for preparing a gel according to the present invention
  • FIG. 2 is a schematic structural view of a left side of an automatic device for preparing a gel according to the present invention
  • FIG. 3 is a schematic structural view of a rear view of an automatic device for preparing a gel according to the present invention.
  • FIG. 4 is a schematic structural diagram of a dye gun and related parts driving the movement of the dye gun according to the present invention
  • Embodiment 2 of the present invention is a schematic structural diagram of Embodiment 2 of the present invention.
  • FIG. 6 is a schematic structural diagram of Embodiment 3 of the present invention.
  • connection should be understood in a broad sense unless explicitly stated and limited otherwise.
  • they may be fixed connections or removable.
  • Connection, or integral connection it can be mechanical or electrical connection; it can be directly connected, or it can be indirectly connected through an intermediate medium, or it can be the internal communication of two elements.
  • connection, or integral connection it can be mechanical or electrical connection; it can be directly connected, or it can be indirectly connected through an intermediate medium, or it can be the internal communication of two elements.
  • the present invention is an automatic gel preparation device, which includes a gel bottle 1, and an upper end of the gel bottle 1 is provided with a liquid inlet tube 2 corresponding to an inlet of the gel bottle 1.
  • the gel bottle 1 With the dye gun 3, the gel bottle 1 can be moved up, down, left and right, and the gel bottle 1 can be rotated along a horizontal axis perpendicular to and intersecting with its own axis.
  • the lower end of the gel bottle 1 is provided with a heating plate 4 and a gel mold 5.
  • the heating plate 4Electromagnetic heating or metal container heating can be used to make the temperature in the gel bottle 1 rise to 80 to 100 degrees quickly. The specific temperature can be adjusted and set according to the actual situation.
  • the gel bottle 1 is made of glass bottle or other metal bottle with anti-corrosive material.
  • a plurality of radiating fins 11 are provided in the middle and lower part of the outer ring of the gel bottle 1, and the height of the radiating fins 11 is greater than 1/2 of the height of the gel bottle.
  • the plurality of radiating fins 11 are evenly distributed on the outer ring of the gel bottle 1.
  • the thickness of the heat sink 11 is close to the thickness of the gel bottle.
  • the number of the heat sink 11 is as much as possible, which can increase the speed of cooling during the gel process and shorten the gel process.
  • the outer ring of the gel bottle 1 is provided with a temperature sensor. 12.
  • the temperature sensor 12 is electrically connected to the control center 6.
  • the temperature sensor 12 is located in the middle of a plurality of heat sinks 11. This area is the height area where the material is mainly mixed.
  • the control center 6 controls the rotary motor 71 to stop the vibration and rotation of the gel bottle 1; more preferably, the gel bottle 1 includes an upper inlet section 13 and a lower portion
  • the fusion section 14 and the inlet section 13 are flared with an upward opening, which is convenient for feeding.
  • the outlet ends of the liquid inlet pipe 2 and the dye gun 3 are set within the cross section of the inlet section 13. After the liquid inlet pipe 2 is discharged, it is vertical.
  • the fusion section 14 is conical with increasing diameter
  • the bottom is big and the bottom is small, to prevent the internal material from splashing during the shaking process, to protect the environment and strong environmental protection, and also to prevent the leakage of the material to affect the operator's safety, because the dye has a certain toxicity, to avoid contact and leakage
  • the lower end of the fusion section 14 is provided with rounded corners. The rounded corners are used to facilitate the full fusion of the internal materials and avoid dead corners.
  • the middle and upper part of the gel bottle 1 is fixed on the holding frame 7, and the intermediate connection part of the inlet section 13 and the fusion section 14 can be set on the holding frame 7, because this part is the position with the smallest diameter, It is convenient to fix, and it is not easy to move up and down.
  • the clamping frame 7 is fixed on the output shaft end of the rotary motor 71.
  • the rotary motor 71 is connected to the control center 6. After filling the gel bottle 1, the gel bottle 1 needs to be carried out. Continuous shaking to ensure the full fusion of the internal materials, and the rotary motor 71 can control the orientation of the gel bottle 1 to form the glue.
  • the rotary motor 71 is set on the moving frame 72 and slides up and down relative to the moving frame 72.
  • the moving frame 72 is far away from the rotating motor 71
  • One side is provided with a lifting motor 73.
  • the lifting motor 73 drives the rotary motor 71 to move up and down with the moving frame 72 through a wire screw mechanism.
  • the lower end of the lifting frame is slidably connected to the support column 75 through the connection plate 74.
  • Side shift The motor 76 and the moving motor 76 drive the connecting plate 74 to move through the screw-screw structure.
  • the setting of the moving motor 76 can connect the plate 74 to drive the moving frame 72 to move left and right, thereby realizing the left and right movement of the gel bottle 1
  • the gel mold 5 and the heating plate 4 are set at the lower end at the same time.
  • the heating plate 4 and the gel mold 5 are arranged in parallel. Therefore, the gel bottle 1 needs to be moved between the two, so it needs to be left and right.
  • the rotary motor 71 realizes the gel bottle.
  • the shaking vibration of 1 and the action of pouring into a gel, the lifting motor 73 realizes the up and down movement of the gel bottle 1, which ensures that the gel bottle 1 descends and contacts the heating plate 4, and rises and separates from the heating plate 4 in total.
  • the right and left of the gel are realized, and the movement of the gel between the heating plate 4 and the gel mold 5 is realized.
  • the whole structure can realize the actions of up, down, left and right and rotation in multiple directions, and can be achieved by the setting of the control center 6.
  • the automatic control of the above actions enhances the degree of automation.
  • an exhaust fan 91 is provided on the side of the support column 75 away from the gel bottle 1, and an adsorption layer 92 is provided between the exhaust fan 91 and the support column 75.
  • the adsorption layer 92 may be an activated carbon layer or a chemical adsorption layer 92. Overflow, under the action of the exhaust fan 91, the gas can be quickly adsorbed by the adsorption layer 92 to avoid environmental pollution caused by the leakage of bad gas.
  • a protective cover is provided on the outside of the entire structure, which is set at the gel position of the gel bottle 1. At the exit, this process is automatically controlled to further improve safety performance, and the setting of the exhaust fan 91 can further accelerate the cooling of the gel bottle 1 and increase the speed of the gel.
  • the gel mold 5 has a trough-shaped structure, and the interior of the gel mold 5 is provided with a plurality of partition plates 52 to divide the interior of the gel mold 5 into a plurality of independent spaces.
  • the order of setting the plurality of diversion openings 53 is that the gel passes through each independent space in order.
  • the gel height in the first independent space is guaranteed to reach the set level.
  • This structure can set the gel to different sizes to meet the needs of different experiments. The size can be divided. Even if the amount of gel is small, the gel setting that reaches a part of the independent space can be guaranteed.
  • the shape and specifications are convenient for storage and subsequent use. More preferably, there are two pairs of flow guides 53 in an independent space. The corners are set to avoid that when the adjacent space is installed, the gel in the independent space does not meet the predetermined requirements, and some of the gel flows into the next independent space.
  • the height of the partition 52 is less than 10mm, and the flow guide 53 Upper end
  • the height of the lowermost end of the gel mold 5 is greater than or equal to 3 mm, and the height of the guide port 53 is 1 to 7 mm, which ensures good fluidity during the gelation process.
  • a semiconductor cooling plate is provided at the lower end of the gel mold 5, and the semiconductor cooling plate and the The control center 6 is electrically connected to help achieve accelerated cooling during the gelation process, shorten the gel time, and improve the efficiency of the gel.
  • the side of the gel mold 5 is provided with a radio frequency identification module 51, and the radio frequency identification module 51 and the control center 6 Electrical connection, read the chip information through the radio frequency identification module 51, determine whether the position of the gel mold 5 is placed correctly, and the type is correct, and control the alarm buzzer to emit an alarm sound, prompting the operator to continue operation or need to be corrected, and whether the type is correct
  • It is the gel shape to be prepared that is, whether the shape of the independent space described above corresponds to the exit end of the gel bottle 1. According to different situations, the position of the gel mold 5 may need to be adjusted each time to determine the first gel space. Position, adjust the direction of gel flow, etc.
  • the lower end of the gel mold 5 is provided with a fixing plate 81, and one side of the fixing plate 81 is provided with a pusher 8.
  • the pusher 8 adopts a structure of an electric push rod or a cylinder, preferably an electric push rod, which has low cost and convenient wiring.
  • the output shaft of the pusher 8 is fixedly connected to the fixed plate 81.
  • the output shaft of the pusher 8 can be extended and retracted to achieve the movement of the fixed plate 81.
  • Positioning columns are provided on both sides of the upper end surface of the fixed plate 81 82.
  • the gel mold 5 is set in a symmetrically positioned positioning column 82 to position the gel mold 5 to ensure the stability of the gel mold 5 during the movement of the fixed plate 81.
  • Guide pillars are provided on both sides of the fixed plate 81 83.
  • the axis of the guide post 83 is set in parallel with the axis of the pusher 8 to ensure the accuracy of the movement of the fixed plate 81.
  • the axis of the push frame is set perpendicular to the longitudinal center plane of the support post 75.
  • the dye gun 3 is an injection needle structure.
  • the dye gun 3 includes a needle 31, a needle tube 32, and a piston 33.
  • the outlet end of the needle 31 is provided at the upper end of the mouth of the gel bottle.
  • the gear 34 is threaded, and the driving gear 35 is engaged with the driven gear 34.
  • the driving gear 35 is rotated by the driving motor 36.
  • the needle tube 32 is fixedly connected to the fixed part of the device.
  • the needle tube 32 is fixed on the upper end of the peristaltic pump 22 to save space.
  • the outside of the needle tube 32 is provided with a scale to facilitate the operator to estimate the use state when starting the dye injection, and other substances such as antibiotics, enzymes, chemical reagents, etc. can also be injected.
  • the control center 6 controls the drive
  • the motor 36 rotates, and the driving gear 35 drives the driven gear 34 to rotate, because the driven gear 34 is screwed with the piston 33.
  • the piston 33 will advance forward to complete the automatic injection of the dye; more preferably Ground, the end of the piston 33 away from the needle 31 is provided with a linear potentiometer 37, the linear electric puller is fixed on the connecting frame, and the connecting frame is provided on the peristaltic pump 22; more preferably Each time, the perfusion needle 31 drops 20ul, and the remaining 0.5ml of the internal liquid needs to be manually replenished.
  • the liquid inlet pipe 2 is in communication with the liquid storage bottle 21, the liquid storage bottle 21 is provided in the bottle holder 211, the bottle holder 211 is provided on the bottom plate 9, the bottle holder 211 is in the shape of a barrel with an upward opening, and the liquid storage bottle 21 is directly placed In the bottle rack 211, the storage bottle 21 can be prevented from shaking.
  • An automatic refilling dosing device 23 is provided outside the liquid inlet pipe 2. The automatic refilling dosing device 23 is connected to the control center 6. A peristalsis is provided between the liquid inlet pipe 2 and the storage bottle. Pump 22, when the liquid needs to be injected, the control center 6 controls the action of the peristaltic pump 22, and the liquid enters the gel bottle 1 through the liquid inlet tube 2.
  • the rubber tube is used for the liquid inlet tube 2.
  • the heating plate 4 and the peristaltic pump 22 are provided.
  • the control center 6 includes a micro-controller microcontroller, a control panel 61, and the micro-controller microcontroller is connected to a peristaltic pump 22, a rotary motor 71, a lifting motor 73, a mobile motor 76, a drive motor 36, a pusher 8, a radio frequency identification module 51, and a control panel 61.
  • the control panel 61 is used for inputting control parameters or outputting operating data.
  • Example 2 As shown in FIG. 5, other structures are the same as the above, except that the dye gun 3 is a micro-sampler structure.
  • the dye gun 3 is provided on the bottom plate 9 through a support rod 38, and the support rod 38 is provided on a heating plate.
  • Embodiment 3 As shown in FIG. 6, other structures are the same as those in Embodiment 2. The difference is that the dye gun 3 is an injection needle structure.
  • the dye gun 3 includes a needle 31, a needle tube 32, and a piston 33.
  • the outlet end of the needle 31 is provided at the condenser.
  • the piston 33 is connected to the stepping motor 39 to complete the injection of dye or other substances.
  • the method of using an automated device for gel preparation is as follows:
  • the first step determine the gel ratio and size, weigh a certain amount of gel powder and add it to the gel bottle 1, the control center 6 starts, and the automatic rehydration quantifier 23 automatically adds liquid according to the size and concentration of the gel;
  • the second step grasp the gel bottle 1 by the holding frame 7 or shake it by rotation, and then move the gel bottle 1 onto the heating plate 4; the gel bottle 1 is rapidly heated to the temperature required for the gel to be provided;
  • the third step through the synergistic effect of the semiconductor cooling sheet and the external exhaust fan 91, the heated gel solution is rapidly cooled to the appropriate temperature required for the later dyes or other substances, and the exhausted gas is adsorbed and filtered through the adsorption layer 92 to reduce the toxicity Material pollution to the surrounding environment;
  • the fourth step start the dye gun 3, add dye or other reagents, drive the piston movement through the drive motor 36, or drive the piston movement through the stepper motor 39 to complete the reagent addition effect, or use a microsampler to complete the reagent addition;
  • Step 5 Place the gel mold 5 on the fixed plate 81.
  • the semiconductor cooling sheet at the bottom pre-cools the gel mold 5 and alarms the buzzer at the same time to remind whether the gel mold 5 is properly placed and positioned.
  • the sixth step the rotation motor 71 is started, after mixing the contents in the gel bottle 1, the gel bottle 1 is moved above the gel mold 5 and poured into the gel mold 5;
  • Step 7 After the cooling gel is coagulated, the gel mold 5 is ejected by the pushing member 8 and the power of the device is turned off.
  • the gel has high accuracy, high efficiency, high product qualification rate, and is not prone to errors.
  • the bottle 1 then use the control panel 61 to input instructions, control the operation of the peristaltic pump 22 through the micro-controller, and automatically add liquid through the automatic replenisher quantifier 23 and according to the size and proportion of the gel.
  • Bottle 1 is vortexed and mixed. After mixing, the lifting motor 73 and the moving motor 76 move at the same time. Place the gel bottle 1 on the heating plate 4.
  • the gel bottle 1 is rapidly heated to 100 ° C.
  • the lifting motor 73 When it moves with the moving motor 76, the gel bottle 1 rises away from the heating plate 4, and moves from one side of the heating plate 4 to above the gel mold 5 for cooling.
  • the semiconductor cooling plate, the heat sink 11 and the exhaust fan 91 cooperate with each other.
  • the heated gel solution is quickly cooled to the appropriate temperature required by the dye, and the exhausted gas is adsorbed and filtered through activated carbon.
  • the dye gun 3 is started, and the piston 33 is pushed to push the dye or Other reagents are injected into the gel solution. At this time, the gel mold 5 is placed on the fixed plate 81.
  • the semiconductor cooling plate at the bottom pre-cools the gel mold 5 and reads the chip information through the radio frequency identification module 51 to determine whether the position of the gel mold 5 is.
  • the gel solution is shaken and mixed by the rotating motor 71, and the gel bottle 1 is moved to the top of the gel plate by the clamping frame 7. After the opening is rotated downward, it is poured into the gel mold 5, and finally the gel coagulates. After that, the gel mold 5 is pushed out by the pusher 8 and the gel is completed.
  • the power of the equipment can be turned off. The entire device is automatically performed according to the set parameters. Manual steps are reduced, the gel preparation is more standardized, and the success of gel production is greatly improved.

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Abstract

凝胶制备用自动化装置及使用方法，属于凝胶制作领域，包括凝胶瓶（1），凝胶瓶（1）的上端设有与凝胶瓶（1）的进口对应设置的进液管（2）和染料枪（3），凝胶瓶（1）可上下左右移动，凝胶瓶（1）可沿与其自身轴线垂直且相交的水平轴线转动，凝胶瓶（1）的下端设有加热板（4）和凝胶模具（5）。本装置避免了人与试剂接触，提升安全系统，同时实现了自动化控制，节约人力，降低劳动强度，节约了凝胶的制备时间，提高了工作效率，使用方便可靠。

Description

凝胶制备用自动化装置及使用方法 技术领域
本发明属于凝胶制作领域，涉及凝胶制备用自动化装置及使用方法。
背景技术
琼脂糖核酸凝胶电泳是分离、鉴定、纯化核酸分子的常用技术，是核酸研究工作中不可缺少的重要分析手段，广泛应用于基础理论研究、农业科学、医学卫生、工业生产、国防科研、法医学和商检等多个领域，在生物化学和分子生物学的教学和科研中具有重要作用。
琼脂糖核酸凝胶电泳可以使带负电荷的核酸分子在电泳时向正极迁移，在电泳过程中需要使用琼脂糖凝胶作为电泳支持介质，发挥分子筛功能，使得大小和构象不同的核酸分子的迁移率出现较大差异，从而达到分离的目的，所以琼脂糖凝胶的质量决定着整个凝胶电泳实验的成功进行，是核酸研究的基础。
传统的琼脂糖凝胶制作基本程序主要包括：选好模具、配置胶液、溶胶、加入核酸染色剂、倒胶、静置凝固，虽然制作的原理、方法简单，但其操作过程中仍存在着的一些问题需要克服和解决：(1)操作过程中加入的核酸染色剂具一定毒性，实验人员长期接触核酸染料，具有较高的致癌风险，严重影响实验人员身体健康，污染环境；(2)由于实际过程中凝胶粉加入的多少、液体量的多少，温度控制等方面都存在人为的主观因素，缺乏标准化，也导致实验电泳图像，出现差别，影响数据分析；(3)整个制作过程都是由人工操作，费时、费力、而且人为的失误往往还会影响实验的整体进展。
发明内容
本发明要解决的问题是在于提供凝胶制备用自动化装置，避免了人与试剂接触，提升安全系统，同时实现了自动化控制，节约人力，降低劳动强度，节约了 凝胶的制备时间，提高了工作效率，使用方便可靠。
为解决上述技术问题，本发明采用的技术方案是：凝胶制备用自动化装置，包括凝胶瓶，所述凝胶瓶的上端设有与所述凝胶瓶的进口对应设置的进液管和染料枪，所述凝胶瓶可上下左右移动，所述凝胶瓶可沿与其自身轴线垂直且相交的水平轴线转动，所述凝胶瓶的下端设有加热板和凝胶模具。
进一步的，所述凝胶瓶外圈的中下部设有多个散热片，所述凝胶瓶的外圈设有温度感应器，所述温度感应器与控制中心电连接。
进一步的，所述凝胶瓶包括上部的入口段和下部的融合段，所述入口段为开口向上的喇叭形，所述进液管与所述染料枪的出口端设在所述入口段的截面范围内，所述融合段为直径逐渐变大的圆锥形，所述融合段的下端设有圆角。
进一步的，所述凝胶瓶的中上部固设在夹持架上，所述夹持架固设在旋转电机的出力轴端，所述旋转电机设在移动架上且相对所述移动架上下滑动连接。
进一步的，所述移动架远离所述旋转电机的一侧设有升降电机，所述升降电机通过丝母丝杠机构驱动所述旋转电机与所述移动架上下滑动连接，所述升降架的下端通过连接板与支撑柱左右滑动连接，所述支撑柱的一侧设有移动电机，所述移动电机通过丝母丝杠结构驱动所述连接板移动。
进一步的，所述凝胶模具为槽形结构，所述凝胶模具的内部设有多个隔板将所述凝胶模具的内部分割为多个独立的空间，每个隔板上端面的一端均设有导流口，多个导流口的设置顺序为凝胶依次通过每个独立的空间的顺序。
进一步的，一个独立空间上的两个导流口对角设置，所述导流口的高度为1～7mm，所述凝胶模具的下端设有半导体冷却板，所述半导体冷却板与控制中心电连接，所述凝胶模具的侧面设有射频识别模块，所述射频识别模块与所述控制中心电连接。
进一步的，所述凝胶模具的下端设有固定板，所述固定板的一侧设有推送件，所述推送件的出力轴与所述固定板固定连接，所述固定板上端面的两侧设有定位柱，所述凝胶模具设在对称设置的所述定位柱内，所述固定板的两侧设有导向柱，所述导向柱的轴线与所述推送件的轴线平行设置。
进一步的，所述染料枪为注射针结构，所述染料枪包括针头、针管和活塞，所述针头的出口端设置在凝胶瓶口的上端，所述活塞与从动齿轮螺纹配合，与所述从动齿轮啮合设置的为主动齿轮，所述主动齿轮通过驱动电机旋转，所述针管与装置的固定部分固定连接。
进一步的，所述进液管与储液瓶连通，所述储液瓶设在瓶架内，所述进液管的外部设有自动补液定量器，所述自动补液定量器与控制中心连接，所述进液管与储瓶之间设有蠕动泵。
使用凝胶制备用自动化装置的方法如下：
第一步：确定凝胶比例和大小，称取一定量的凝胶粉加入到凝胶瓶中，控制中心启动，自动补液定量器根据凝胶的大小、浓度自动加液；
第二步：夹持架抓取凝胶瓶左右或旋转震荡混匀，并将凝胶瓶移入到加热板上，凝胶瓶被迅速升温至所配凝胶需要的温度；
第三步：通过半导体制冷片及外部排风扇协同作用，将加热的凝胶液快速降温至后期染料或其他物质所需的适宜温度，同时排出的气体通过吸附层进行吸附过滤，以减少有毒物质对周围环境的污染；
第四步：启动染料枪，加入染料剂或其他试剂，通过驱动电机驱动活塞运动，或者通过步进电机驱动活塞运动完成加试剂的效果，或采用微量进样器完成加试剂；
第五步：将凝胶模具放到固定板上，底部半导体制冷片将凝胶模具预冷，同时报警蜂鸣装置，提醒凝胶板是否放置和位置是否得当；
第六步：旋转电机启动，将凝胶瓶内的物质混匀后，将凝胶瓶移动至凝胶模具的上方，倒入凝胶模具中；
第七步：降温凝胶凝结后，凝胶模具在推送件的作用下弹出，关闭设备电源。
与现有技术相比，本发明具有的优点和积极效果是：1、整个装置按照设定的参数自动进行，减少了人工操作步骤，凝胶制备更加标准化，大大提高制胶的成功率，制胶过程只需人工凝胶粉称量，其他步骤完全仪器完成，无需人员守候操 作，减少了有毒物质的接触，降低了操作人员意外伤害的风险，而且结构使用简便，自动化、智能化程度高，提高凝胶制备的效率；2、设置染料枪，可实现染料或其他试剂的自动添加，减少实验人员长期接触核酸染色剂，降低致癌风险，防止影响实验人员身体健康，防止污染环境，提高使用安全性和便利性，而且采用驱动电机通过控制中心自动上加料，节省人力，提升工作效率；3、设置旋转电机、升降电机和移动电机，实现了凝胶瓶的上下左右移动和旋转，保证了加热、摇晃以及倒胶的功能，自动化程度高，降低了劳动强度，同时提升了工作效率；4、在凝胶瓶的外圈设置温度感应器，在储液瓶的外部设置动补液定量器，对温度控制和对加液量的控制均通过控制中心实现，减少人为主观因素，提高标准化，防止实验电泳图像出现差别，防止影响收据分析，降低使用局限性，而且无需人工操作，省时省力，防止人为操作产生误差影响实验整体进展，提高实用性；5、设置排风扇和吸附层，在有毒气体不小心泄露的时候，可及时的进行处理和吸附，保证对操作者安全性的同时，保护了环境，环保性更强，安全系数更高。
附图说明
构成本发明的一部分的附图用来提供对本发明的进一步理解，本发明的示意性实施例及其说明用于解释本发明，并不构成对本发明的不当限定。在附图中：
图1是本发明凝胶制备用自动化装置正视的结构示意图；
图2是本发明凝胶制备用自动化装置左视的结构示意图；
图3是本发明凝胶制备用自动化装置后视的结构示意图；
图4是本发明染料枪及驱动其运动的相关零件的结构示意图；
图5是本发明实施例2的结构示意图；
图6是本发明实施例3的结构示意图。
附图标记：
1-凝胶瓶；11-散热片；12-温度感应器；13-入口段；14-融合段；2-进液管；21-储液瓶；211-瓶架；22-蠕动泵；23-自动补液定量器；3-染液枪；31-针头；32-针管；33-活塞；34-从动齿轮；35-主动齿轮；36-驱动电机；37-直线电位器； 38-支杆；381-导向槽；39-步进电机；4-加热板；5-凝胶模具；51-射频识别模块；52-隔板；53-导流口；6-控制中心；61-控制面板；7-夹持架；71-旋转电机；72-移动架；73-升降电机；74-连接板；75-支撑柱；76-移动电机；8-推送件；81-固定板；82-定位柱；83-导向柱；9-底板；91-排风扇；92-吸附层。
具体实施方式
需要说明的是，在不冲突的情况下，本发明中的实施例及实施例中的特征可以相互组合。
在本发明的描述中，需要理解的是，术语“中心”、“纵向”、“横向”、“上”、“下”、“前”、“后”、“左”、“右”、“竖直”、“水平”、“顶”、“底”、“内”、“外”等指示的方位或位置关系为基于附图所示的方位或位置关系，仅是为了便于描述本发明和简化描述，而不是指示或暗示所指的装置或元件必须具有特定的方位、以特定的方位构造和操作，因此不能理解为对本发明的限制。此外，术语“第一”、“第二”等仅用于描述目的，而不能理解为指示或暗示相对重要性或者隐含指明所指示的技术特征的数量。由此，限定有“第一”、“第二”等的特征可以明示或者隐含地包括一个或者更多个该特征。在本发明的描述中，除非另有说明，“多个”的含义是两个或两个以上。
在本发明的描述中，需要说明的是，除非另有明确的规定和限定，术语“安装”、“相连”、“连接”应做广义理解，例如，可以是固定连接，也可以是可拆卸连接，或一体地连接；可以是机械连接，也可以是电连接；可以是直接相连，也可以通过中间媒介间接相连，可以是两个元件内部的连通。对于本领域的普通技术人员而言，可以通过具体情况理解上述术语在本发明中的具体含义。
下面结合附图对本发明的具体实施例做详细说明。
如图1、图2和图3所示，本发明为凝胶制备用自动化装置，包括凝胶瓶1，凝胶瓶1的上端设有与凝胶瓶1的进口对应设置的进液管2和染料枪3，凝胶瓶1可上下左右移动，凝胶瓶1可沿与其自身轴线垂直且相交的水平轴线转动，凝胶瓶1的下端设有加热板4和凝胶模具5，加热板4可采用电磁加热的方式，也可以采用金属容器加热的方式，使得凝胶瓶1内的温度快速升温到80到100度 之间，具体的温度可根据实际情况进行调节和设定；优选地，凝胶瓶1采用玻璃瓶或其他防腐蚀材质的金属瓶。
优选地，凝胶瓶1外圈的中下部设有多个散热片11，散热片11的高度大于凝胶瓶高度的1/2，多个散热片11均布在凝胶瓶1的外圈，散热片11的厚度与凝胶瓶的厚度接近设置，散热片11的数量尽量多，可提升凝胶过程中降温的速度，缩短凝胶过程，凝胶瓶1的外圈设有温度感应器12，温度感应器12与控制中心6电连接，温度感应器12设在多个散热片11中间，此区域是物料混合的主要所在的高度区域，设置在此区域感应的凝胶瓶1内部的温度更加精确，达到设定的温度数值后，控制中心6再控制旋转电机71进行凝胶瓶1的停止振动和旋转倾倒等动作；更优选地，凝胶瓶1包括上部的入口段13和下部的融合段14，入口段13为开口向上的喇叭形，方便进料，进液管2与染料枪3的出口端设在入口段13的截面范围内，进液管2的出料后竖直向下设置，融合段14为直径逐渐变大的圆锥形，下面大上面小，防止内部的物质在晃动的过程中溅出，保护环境，环保性强，同时也避免物质外泄对操作者安全造成影响，因为染料具有一定的毒性，要避免接触和外泄，融合段14的下端设有圆角，采用圆角方便内部的物质充分融合，避免出现死角。
优选地，凝胶瓶1的中上部固设在夹持架7上，可将入口段13和融合段14的中间连接部位设在夹持架7上，因为此部位是直径最细的位置，固定方便，而且上下不容易窜动，夹持架7固设在旋转电机71的出力轴端，旋转电机71与控制中心6连接，在凝胶瓶1内注入物质后需要对凝胶瓶1进行不断的摇晃，保证内部物质的充分融合，而且旋转电机71可控制凝胶瓶1朝向设置，出胶成型，旋转电机71设在移动架72上且相对移动架72上下滑动连接，上下移动后，可保证凝胶瓶1落在加热板4上，对内部的物质进行加热处理，实现凝胶瓶1内物质的快速升温，促进凝胶的形成；更优选地，移动架72远离旋转电机71的一侧设有升降电机73，升降电机73通过丝母丝杠机构驱动旋转电机71与移动架72上下滑动连接，升降架的下端通过连接板74与支撑柱75左右滑动连接，支撑柱75的一侧设有移动电机76，移动电机76通过丝母丝杠结构驱动连接板74移动，移动电机76的设置可连接板74带动移动架72左右移动，进而实现了凝胶瓶1的左右移动，凝胶瓶1的下端同时设置凝胶模具5和加热板4，加热板4和凝胶 模具5平行设置，因此凝胶瓶1需要在二者之间移动，因此需要左右一定，旋转电机71实现了对凝胶瓶1的摇晃振动以及倾倒成凝胶的动作，升降电机73实现了凝胶瓶1的上下升降，保证了凝胶瓶1下降与加热板4接触，上升与加热板4分离的总过，移动电机76实现了凝胶的左右一定，实现了凝胶在加热板4和凝胶模具5之间的移动，整个结构可实现上下、左右和旋转多个方向的动作，通过控制中心6的设置可实现以上动作的自动化控制，提升自动化程度。
更优选地，支撑柱75远离凝胶瓶1的一侧设有排风扇91，排风扇91与支撑柱75之间设有吸附层92，吸附层92可选用活性炭层或化学吸附层92，如有气体溢出，在排风扇91的作用下，气体可快速的被吸附层92吸附，避免不良气体泄漏造成环境污染，更优选地，整个结构的外部设有防护罩，在凝胶瓶1凝胶的位置设置出口，这个过程实现自动化控制，进一步提升安全性能，而排风扇91的设置可进一步加快凝胶瓶1的降温，提升凝胶的速度。
优选地，凝胶模具5为槽形结构，凝胶模具5的内部设有多个隔板52将凝胶模具5的内部分割为多个独立的空间，每个隔板52上端面的一端均设有导流口53，多个导流口53的设置顺序为凝胶依次通过每个独立的空间的顺序，在凝胶的过程中，保证在第一个独立空间的凝胶高度达到设定的高度后再通过导流口53流向第二个独立空间，依次进行，直至多个空间均凝胶完成，此结构可将凝胶设置成大小不同的规格，满足不同试验的需求，而且空间的大小可以进行分割，即使有时凝胶量小，也可保证到达部分独立空间的凝胶设定，形状规格，方便存储和后续使用；更优选地，一个独立空间上的两个导流口53对角设置，避免相邻设置的时候，所在的独立空间的凝胶还没达到预定要求后就有部分凝胶流到下一个独立的空间内，隔板52的高度为10mm以下，导流口53的上端面到凝胶模具5最下端的高度为大于等于3mm,导流口53的高度为1～7mm，保证凝胶过程中良好的流动性，凝胶模具5的下端设有半导体冷却板，半导体冷却板与控制中心6电连接，有助于实现凝胶的过程中加速冷却，缩短凝胶的时间，提升凝胶的效率，凝胶模具5的侧面设有射频识别模块51，射频识别模块51与控制中心6电连接，通过射频识别模块51读取芯片信息，判断凝胶模具5位置是否放好，类型是否正确，并控制报警蜂鸣器发出报警音，提示操作者继续操作还是需要纠正，类型是否正确就是预备制作的凝胶形，即上述的独立空间的形状是否与凝胶瓶1 的出口端进行对应，根据不同的情况，每次可能需要调节凝胶模具5的位置，确定第一凝胶空间的位置，调节凝胶的流动方向等。
优选地，凝胶模具5的下端设有固定板81，固定板81的一侧设有推送件8，推送件8采用电动推杆或者气缸的结构，优选为电动推杆，成本低，接线方便，推送件8的出力轴与固定板81固定连接，推送件8的出力轴做伸出和缩回的动作，即可实现固定板81的移动，固定板81上端面的两侧设有定位柱82，凝胶模具5设在对称设置的定位柱82内，对凝胶模具5进行定位，保证固定板81移动过程中，凝胶模具5的稳定性，固定板81的两侧设有导向柱83，导向柱83的轴线与推送件8的轴线平行设置，保证了固定板81移动的精度，推送架的轴线与支撑柱75的纵向中心面垂直设置。
优选地，如图4所示，染料枪3为注射针结构，染料枪3包括针头31、针管32和活塞33，针头31的出口端设置在凝胶瓶1口的上端，活塞33与从动齿轮34螺纹配合，与从动齿轮34啮合设置的为主动齿轮35，主动齿轮35通过驱动电机36旋转，针管32与装置的固定部分固定连接，针管32固定在蠕动泵22的上端，节省空间，针管32的外部设有刻度，方便操作人员在开始进行染料注入的时候进行使用状态的估算，也可以注入其他物质，如抗生素、酶、化学试剂等，需要注射染料的时候，控制中心6控制驱动电机36旋转，进而主动齿轮35驱动从动齿轮34旋转，因为从动齿轮34与活塞33螺纹连接，从动齿轮34转动的过程中，活塞33会向前推进，完成染料的自动注射；更优选地，活塞33远离针头31的一端设有直线电位器37，直线电拉器固定在连接架上，连接架设置在蠕动泵22上；更优选地，每次灌注针头31滴出20ul，内部液体剩余0.5ml时候需手动补充。
优选地，进液管2与储液瓶21连通，储液瓶21设在瓶架211内，瓶架211设在底板9上，瓶架211为开口向上的桶形，储液瓶21直接放置在瓶架211内可防止储液瓶21晃动，进液管2的外部设有自动补液定量器23，自动补液定量器23与控制中心6连接，进液管2与储瓶之间设有蠕动泵22，需要打入液体的时候，控制中心6控制蠕动泵22动作，液体通过进液管2进入到凝胶瓶1内，进液管2采用橡胶软管，加热板4与蠕动泵22设在凝胶瓶1的两侧；更优选地， 所有的两件均固定在底板9上，使得结构构成一个撬装结构，只需组装一次，各个零件之间的相对位置关系即可保证，方便后续的转运和搬移。
控制中心6包括包括微控单片机、控制面板61，微控单片机与蠕动泵22、旋转电机71、升降电机73、移动电机76、驱动电机36、推送件8、射频识别模块51及控制面板61连接，接收控制面板61的输入信号，并对蠕动泵22、旋转电机71、升降电机73、移动电机76、驱动电机36、推送件8、射频识别模块51进行控制，微控单片机为数据处理交换中心,用于接收、分析、处理、发送数据及指令，控制面板61用于输入控制参数或输出运行数据。
实施例2：如图5所示，其他结构与上述相同，不同的是，染料枪3为微量进样器结构，染料枪3通过支杆38设在底板9上，支杆38设在加热板4远离凝胶瓶1的一侧，支架上端设置导向槽381，染料枪3设在导向槽381内且倾斜向下设置。
实施例3：如图6所示，其他结构与实施例2相同，不同的是染料枪3为注射针结构，染料枪3包括针头31、针管32和活塞33，针头31的出口端设置在凝胶瓶1口的上端，活塞33与步进电机39连接，完成染料或其他物质的注射。
使用凝胶制备用自动化装置的方法如下：
第一步：确定凝胶比例和大小，称取一定量的凝胶粉加入到凝胶瓶1中，控制中心6启动，自动补液定量器23根据凝胶的大小、浓度自动加液；
第二步：夹持架7抓取凝胶瓶1左右或旋转震荡混匀，并将凝胶瓶1移入到加热板4上，凝胶瓶1被迅速升温至所配凝胶需要的温度；
第三步：通过半导体制冷片及外部排风扇91协同作用，将加热的凝胶液快速降温至后期染料或其他物质所需的适宜温度，同时排出的气体通过吸附层92进行吸附过滤，以减少有毒物质对周围环境的污染；
第四步：启动染料枪3，加入染料剂或其他试剂，通过驱动电机36驱动活塞运动，或者通过步进电机39驱动活塞运动完成加试剂的效果，或采用微量进样器完成加试剂；
第五步：将凝胶模具5放到固定板81上，底部半导体制冷片将凝胶模具5预冷，同时报警蜂鸣装置，提醒凝胶模具5是否放置和位置是否得当；
第六步：旋转电机71启动，将凝胶瓶1内的物质混匀后，将凝胶瓶1移动至凝胶模具5的上方，倒入凝胶模具5中；
第七步：降温凝胶凝结后，凝胶模具5在推送件8的作用下弹出，关闭设备电源。
在实际工作过程中，严格按照以上步骤进行，凝胶精确度高，效率高，产品合格率高，操作不易出错，首先按凝胶比例和大小，称取一定量的凝胶粉加入到凝胶瓶1中，然后使用控制面板61输入指令，通过微控单片机控制蠕动泵22动作，通过自动补液定量器23并根据凝胶的大小、比例进行自动加液，然后打开旋转电机71，驱动凝胶瓶1旋转震荡混匀，混匀后升降电机73和移动电机76同时动作，将凝胶瓶1放置到加热板4上，凝胶瓶1被迅速升温至100℃，加热完成后，升降电机73和移动电机76动作，凝胶瓶1上升离开加热板4的同时，从加热板4的一侧运动到凝胶模具5的上方进行冷却，通过半导体冷却板、散热片11以及排风扇91协同作用，将加热的凝胶液快速降温至染料所需的适宜温度，同时排出的气体通过活性炭进行吸附过滤，染料枪3启动，活塞33推送，将染料或其他试剂注入凝胶液中，此时凝胶模具5放置到固定板81上，底部半导体冷却板将凝胶模具5预冷，通过射频识别模块51读取芯片信息，判断凝胶模具5位置是否放好，凝胶液通过旋转电机71震荡混匀后通过夹持架7将凝胶瓶1移动至凝胶板的上方后，开口旋转向下后倒入凝胶模具5中，最后凝胶凝结后，凝胶模具5通过推送件8推出，凝胶完成，关闭设备电源即可，整个装置按照设定的参数自动进行，减少了人工操作步骤，凝胶制备更加标准化，大大提高制胶的成功率，制胶过程只需人工凝胶粉称量，其他步骤完全仪器完成，无需人员守候操作，减少了有毒物质的接触，降低了操作人员意外伤害的风险，而且结构使用简便，自动化、智能化程度高，提高凝胶制备的效率。
以上对本发明的一个实施例进行了详细说明，但所述内容仅为本发明的较佳实施例，不能被认为用于限定本发明的实施范围。凡依本发明申请范围所作的均等变化与改进等，均应仍归属于本发明的专利涵盖范围之内。

Claims (10)

  1. 凝胶制备用自动化装置，其特征在于：包括凝胶瓶，所述凝胶瓶的上端设有与所述凝胶瓶的进口对应设置的进液管和染料枪，所述凝胶瓶可上下左右移动，所述凝胶瓶可沿与其自身轴线垂直且相交的水平轴线转动，所述凝胶瓶的下端设有加热板和凝胶模具。
  2. 根据权利要求1所述的凝胶制备用自动化装置，其特征在于：所述凝胶瓶外圈的中下部设有多个散热片，所述凝胶瓶的外圈设有温度感应器，所述温度感应器与控制中心电连接。
  3. 根据权利要求1或2所述的凝胶制备用自动化装置，其特征在于：所述凝胶瓶包括上部的入口段和下部的融合段，所述入口段为开口向上的喇叭形，所述进液管与所述染料枪的出口端设在所述入口段的截面范围内，所述融合段为直径逐渐变大的圆锥形，所述融合段的下端设有圆角。
  4. 根据权利要求1或2任一项所述的凝胶制备用自动化装置，其特征在于：所述凝胶瓶的中上部固设在夹持架上，所述夹持架固设在旋转电机的出力轴端，所述旋转电机设在移动架上且相对所述移动架上下滑动连接，所述移动架远离所述旋转电机的一侧设有升降电机，所述升降电机通过丝母丝杠机构驱动所述旋转电机与所述移动架上下滑动连接，所述升降架的下端通过连接板与支撑柱左右滑动连接，所述支撑柱的一侧设有移动电机，所述移动电机通过丝母丝杠结构驱动所述连接板移动。
  5. 根据权利要求1或2任一项所述的凝胶制备用自动化装置，其特征在于：所述凝胶模具为槽形结构，所述凝胶模具的内部设有多个隔板将所述凝胶模具的内部分割为多个独立的空间，每个隔板上端面的一端均设有导流口，多个导流口的设置顺序为凝胶依次通过每个独立的空间的顺序。
  6. 根据权利要求5所述的凝胶制备用自动化装置，其特征在于：一个独立空间上的两个导流口对角设置，所述导流口的高度为1～7mm，所述凝胶模具的下端设有半导体冷却板，所述半导体冷却板与控制中心电连接，所述凝胶模具的侧面设有射频识别模块，所述射频识别模块与所述控制中心电连接。
  7. 根据权利要求6所述的凝胶制备用自动化装置，其特征在于：所述凝胶模具的下端设有固定板，所述固定板的一侧设有推送件，所述推送件的出力轴与所述固定板固定连接，所述固定板上端面的两侧设有定位柱，所述凝胶模具设在对称设置的所述定位柱内，所述固定板的两侧设有导向柱，所述导向柱的轴线与所述推送件的轴线平行设置。
  8. 根据权利要求1、2、6或7任一项所述的凝胶制备用自动化装置，其特征在于：所述染料枪为注射针结构，所述染料枪包括针头、针管和活塞，所述针头的出口端设置在凝胶瓶口的上端，所述活塞与从动齿轮螺纹配合，与所述从动齿轮啮合设置的为主动齿轮，所述主动齿轮通过驱动电机旋转，所述针管与装置的固定部分固定连接。
  9. 根据权利要求1、2、6或7任一项所述的凝胶制备用自动化装置，其特征在于：所述进液管与储液瓶连通，所述储液瓶设在瓶架内，所述进液管的外部设有自动补液定量器，所述自动补液定量器与控制中心连接，所述进液管与储瓶之间设有蠕动泵。
  10. 使用权利要求1所述的凝胶制备用自动化装置的方法，其特征在于：
    第一步：确定凝胶比例和大小，称取一定量的凝胶粉加入到凝胶瓶中，控制中心启动，自动补液定量器根据凝胶的大小、浓度自动加液；
    第二步：夹持架抓取凝胶瓶左右或旋转震荡混匀，并将凝胶瓶移入到加热板上，凝胶瓶被迅速升温至所配凝胶需要的温度；
    第三步：通过半导体制冷片及外部排风扇协同作用，将加热的凝胶液快速降温至后期染料或其他物质所需的适宜温度，同时排出的气体通过吸附层进行吸附过滤，以减少有毒物质对周围环境的污染；
    第四步：启动染料枪，加入染料剂或其他试剂，通过驱动电机驱动活塞运动，或者通过步进电机驱动活塞运动完成加试剂的效果，或采用微量进样器完成加试剂；
    第五步：将凝胶模具放到固定板上，底部半导体制冷片将凝胶模具预冷，同时报警蜂鸣装置，提醒凝胶板是否放置和位置是否得当；
    第六步：旋转电机启动，将凝胶瓶内的物质混匀后，将凝胶瓶移动至凝胶模具的上方，倒入凝胶模具中；
    第七步：降温凝胶凝结后，凝胶模具在推送件的作用下弹出，关闭设备电源。
PCT/CN2018/092086 2018-06-14 2018-06-21 凝胶制备用自动化装置及使用方法 WO2019237409A1 (zh)

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