WO2019207817A1 - Armoire de sécurité - Google Patents

Armoire de sécurité Download PDF

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Publication number
WO2019207817A1
WO2019207817A1 PCT/JP2018/038280 JP2018038280W WO2019207817A1 WO 2019207817 A1 WO2019207817 A1 WO 2019207817A1 JP 2018038280 W JP2018038280 W JP 2018038280W WO 2019207817 A1 WO2019207817 A1 WO 2019207817A1
Authority
WO
WIPO (PCT)
Prior art keywords
safety cabinet
excitation light
work space
cabinet according
light source
Prior art date
Application number
PCT/JP2018/038280
Other languages
English (en)
Japanese (ja)
Inventor
憲一 相馬
Original Assignee
株式会社日立産機システム
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 株式会社日立産機システム filed Critical 株式会社日立産機システム
Publication of WO2019207817A1 publication Critical patent/WO2019207817A1/fr

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Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L1/00—Enclosures; Chambers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M1/00—Apparatus for enzymology or microbiology
    • F—MECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
    • F24—HEATING; RANGES; VENTILATING
    • F24F—AIR-CONDITIONING; AIR-HUMIDIFICATION; VENTILATION; USE OF AIR CURRENTS FOR SCREENING
    • F24F7/00—Ventilation
    • F24F7/04—Ventilation with ducting systems, e.g. by double walls; with natural circulation
    • F24F7/06—Ventilation with ducting systems, e.g. by double walls; with natural circulation with forced air circulation, e.g. by fan positioning of a ventilator in or against a conduit
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence

Definitions

  • the present invention relates to a safety cabinet used for research such as regenerative medicine and pathogens.
  • a safety cabinet is used when handling cells in regenerative medicine where cells are cultured.
  • Patent Document 1 provides a blow-out HEPA filter at the top of the work space, a front shutter that can be opened and closed at the front of the work space, a rear suction portion at the rear wall, and a lower front.
  • a front suction part uniformly supplying air from the HEPA filter for blowing to the work space, and sucking air from the front suction part and the rear suction part of the work table that forms the bottom surface of the work space.
  • the object of the present invention is to provide a safety cabinet that can easily determine that the work space has been contaminated and can easily confirm that the work space has been decontaminated.
  • a safety cabinet having a front plate and a work opening on a front surface of a work space, and supplying clean air from above to the work space, wherein the work space is irradiated with an excitation light source and excitation light from the excitation light source. Imaging means for observing the generated fluorescence.
  • the present invention it can be easily determined that the work space of the safety cabinet has been contaminated, and it can be easily confirmed that it has been decontaminated.
  • FIG. It is an example of the external appearance front view which shows the safety cabinet of Example 1.
  • FIG. It is an example of side sectional drawing which shows the safety cabinet of Example 1.
  • FIG. It is a figure which shows the flow of the air at the time of operation
  • movement of the safety cabinet of FIG. It is a figure which shows the working space of the safety cabinet of Example 1.
  • FIG. It is a figure which shows the relationship between the excitation light of Example 1, and fluorescence.
  • FIG. It is a figure which shows the arrangement
  • FIG. It is a figure which shows the safety cabinet of Example 3.
  • FIG. 1 shows a schematic front view of the safety cabinet.
  • FIG. 2 is a schematic side view of the safety cabinet when the A-A ′ cross section of FIG. 1 is viewed from the right side.
  • An opening is provided in the central area of the casing 101 of the safety cabinet 100, and a work space 104 is provided in the back thereof.
  • a front plate 102 is provided on the front side of the work space 104 so as to block the upper portion of the opening, and a work opening 103 is provided on the lower side thereof. The operator puts his hand into the work space 104 from the work opening 103. Do the work.
  • the front plate 102 is formed of a transparent material such as glass, and an operator can visually observe the work through the front plate.
  • a substantially flat work stage 105 is provided on the bottom surface of the work space 104, and the worker performs work on the work stage.
  • An intake port 107 leading to the lower side is provided on the front side of the work stage 105 and in the vicinity of the work opening 103.
  • the air inlet 107 is formed by a slit extending in the left-right direction of the housing along the work opening 103.
  • a back channel 108 that leads from the air inlet 107 to the upper part of the housing is provided.
  • a blow-out side FFU (fan filter unit) 109 is provided above the work space 104.
  • the blowout side FFU 109 includes a fan rotated by a motor and a filter for removing fine particles, for example, a HEPA filter 109A, and blows clean air from which fine particles have been removed to the work space 104.
  • An exhaust-side FFU (fan filter unit) 110 is provided on the upper portion of the casing 101, and a part of air is removed through a filter, for example, a HEPA filter 110A, and discharged to the outside of the apparatus.
  • Fig. 3 shows the air flow with arrows when the safety cabinet is operating.
  • the air 90 sucked from the air inlet 107 on the front side of the work stage 105 passes through the lower part of the casing, the rear passage 108, and the upper part of the casing as indicated by reference numeral 91, and then enters the work space 104 from the blowout side FFU 109. Be blown.
  • the work space 104 is maintained in a clean state by blowing clean air from which fine particles have been removed by the HEPA filter 109A of the blow-out side FFU 109 into the work space 104. At this time, the air in the work space may leak to the outside only by the air flow to the work space 104 indicated by reference numeral 92.
  • the discharge side FFU 110 is provided, and part of the air is discharged to the outside through the HEPA filter 110A.
  • the pressure in the work space decreases, and an air flow 94 is generated from the outside to be introduced into the inside through the work opening 103 below the front plate 102. If this air flow 94 flows into the work space as it is, the cleanliness of the work space is lowered.
  • the air volume of the air flow 92 blown out from the blowout side FFU 109 to the work space and the air volume of the air flow 93 discharged outside from the discharge side FFU 110 all of the air 94 flowing in from the work opening 103 is obtained.
  • the air 92 that sucks most of the air 92 sent to the work space from the air inlet 107 prevents the air 94 from flowing into the work space 104 from flowing into the work space 104 by the air flow 92 blown out to the work space 104.
  • Wall air barrier
  • FIG. 4 shows a characteristic configuration of this embodiment.
  • an excitation light source 20 and imaging means for detecting fluorescence generated from mold, bacteria, etc. for example, a camera 21 are provided.
  • the excitation light source 20 may be an ultraviolet lamp, LED, laser, or the like.
  • reference numeral 22 denotes a band-pass filter that limits the wavelength of light incident on the camera.
  • the excitation light source 20 emits light having a wavelength of 420 nm or less, for example, ultraviolet light.
  • the contaminant 24 generates fluorescence specific to mold and fluorescence specific to bacteria. The generated fluorescence is imaged by the camera 21, and the presence or absence of contamination and the state of contamination removal are confirmed.
  • FIG. 5 shows the relationship between excitation light and generated fluorescence.
  • the excitation light 30 is irradiated with ultraviolet light having a wavelength of 420 nm or less.
  • Reference numeral 32 indicates fluorescence generated from microorganisms
  • reference numeral 34 indicates fluorescence generated from bacteria.
  • molds and bacteria generate different types of fluorescence depending on the wavelength of the irradiated light source.
  • the bandpass filter 22 may be switched to change the wavelength of the transmitted light.
  • a suitable decontamination method can be selected, for example, by considering the antibacterial acid, anti-alkali, anti-alcohol and the like for the identified microorganism.
  • the unillustrated illumination provided in the work space may be turned off or dimmed, or a transparent front plate or work opening may be shielded.
  • the fluorescence detection sensitivity can be improved.
  • the excitation light from the excitation light source 20 is irradiated in the operating state of the safety cabinet, that is, in the state where the air barrier is formed.
  • FIG. 6 shows the situation where the cells are irradiated with excitation light.
  • the cell 40 includes a nucleus 42, a protoplasm 43 surrounding the nucleus, and a cell membrane 44 surrounding the protoplasm.
  • the fluorescence A by the nucleus, the fluorescence B by the protoplasm, and the fluorescence C by the cell membrane are generated.
  • a, b, and c represent sensitizing reagents for nuclei, protoplasm, and cell membrane, respectively.
  • a sensitizing reagent that enhances fluorescence from a specific microorganism may be sprayed.
  • the sensitizing reagent can be selected for each contaminant, for example, for each microorganism and bacterium, or for each cell site, for example, for each nucleus, protoplasm, or cell membrane. Spraying of the sensitizing reagent can be performed by providing a sensitizing reagent spraying means in the work space 104.
  • FIG. 7 shows an example of a block diagram of an electrical system for detecting contamination.
  • the fluorescence image captured by the camera 21 is input to the image processing unit 51.
  • the image processing unit 51 detects the intensity of fluorescence at each position of the image.
  • the fluorescence intensity may be detected for each wavelength.
  • the image input from the camera or the detection result of the fluorescence intensity can be stored in the storage unit 52 and read out from the storage unit 52 as necessary.
  • the determination unit 53 compares the detection result of the fluorescence intensity with a preset threshold value, and determines that it is a contaminant if it is greater than or equal to the threshold value. By comparing the fluorescence intensity detection result for each wavelength with a preset threshold value, the type of contaminant can also be determined.
  • the display unit 54 displays the presence or absence of contaminants. Contaminants may be displayed on the map indicating the work space by storing the detection result by combining the detected fluorescence wavelength and the position information read from the image. Further, a subsequent response instruction, for example, an instruction to re-execute the cleaning process may be displayed together with the display of the contaminant.
  • the display is not limited to image display, but may be instructed by voice. In the case where a display is provided in the safety cabinet, it is only necessary to display the determination result of the contaminant and the subsequent response instruction.
  • the occurrence of mold and bacteria, and the growth state can be monitored by observing the work space with a camera at predetermined time intervals. Then, the contamination status data can be accumulated by connecting the camera to the server via the communication line. In addition, by connecting to the Internet, remote monitoring can be performed from a location away from the safety cabinet.
  • the present embodiment it can be easily determined that the work space of the safety cabinet has been contaminated, and it can be easily confirmed that it has been reliably decontaminated.
  • FIG. 8 shows an example of the safety cabinet of the second embodiment.
  • the second embodiment is provided with a plurality of cameras in order to observe substantially the entire work space 104.
  • an excitation light source 20 is arranged at the center of the ceiling of the work space. Then, two cameras 21 including band pass filters 22 are arranged on both sides of the excitation light source 20. By using a plurality of cameras, a wide range of contaminants 24 can be observed. More cameras may be arranged according to the light receiving range of the camera. A plurality of excitation light sources 20 may be provided.
  • FIG. 9 shows an example of the safety cabinet of the third embodiment.
  • an excitation light source and a camera are mounted on a movable mechanism such as a robot arm in order to observe almost the entire work space 104.
  • a robot arm an arm that can be moved by incorporating a motor
  • a camera 21 including an excitation light source 20 and a bandpass filter is attached to the tip of the robot arm 25.
  • a wide range of contaminants 24 can be observed by moving the robot arm by the control unit and changing the positions and orientations of the excitation light source 20 and the camera 21.
  • the excitation light source and the imaging means are attached to the movable mechanism such as the robot arm, it is possible to observe a wide range of contaminants in the work space, and to confirm the contamination and decontamination of the work space. Can be done. Moreover, irradiation with excitation light and observation of generated fluorescence can be performed at a position close to the work stage, and the detection sensitivity of contaminants can be improved.
  • Excitation light source 21
  • Camera 22
  • Band pass filter 24
  • Contaminant 26
  • Robot arm 30
  • Excitation light 32
  • Fluorescence due to microorganisms 34
  • Fluorescence due to bacteria 40
  • Cell 42
  • Nucleus 43
  • Protoplasm 44
  • Cell membrane 51
  • Image processing unit 52
  • Determination unit 54
  • Display unit 100
  • Safety Cabinet 101 Housing 102
  • Front plate 103
  • Work opening 104 Work space
  • Intake port 108
  • Rear flow path 109
  • Outlet side FFU (fan filter unit)
  • Outlet side HEPA filter 110A discharge side HEPA filter

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  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Organic Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Pathology (AREA)
  • Clinical Laboratory Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Sustainable Development (AREA)
  • Immunology (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • General Physics & Mathematics (AREA)
  • Genetics & Genomics (AREA)
  • Analytical Chemistry (AREA)
  • Combustion & Propulsion (AREA)
  • Mechanical Engineering (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Ventilation (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Devices For Use In Laboratory Experiments (AREA)

Abstract

L'invention concerne une armoire de sécurité capable de détecter facilement une contamination et de confirmer facilement la décontamination d'un espace de travail. L'armoire de sécurité a un panneau de surface avant et une ouverture de travail au niveau de la surface avant de l'espace de travail. L'armoire de sécurité fournit de l'air purifié dans l'espace de travail par le haut. L'espace de travail est pourvu d'une source de lumière d'excitation et d'un moyen d'imagerie pour observer la lumière fluorescente générée par le rayonnement de lumière d'excitation provenant de la source de lumière d'excitation.
PCT/JP2018/038280 2018-04-25 2018-10-15 Armoire de sécurité WO2019207817A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2018083952A JP2019187309A (ja) 2018-04-25 2018-04-25 安全キャビネット
JP2018-083952 2018-04-25

Publications (1)

Publication Number Publication Date
WO2019207817A1 true WO2019207817A1 (fr) 2019-10-31

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WO (1) WO2019207817A1 (fr)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113295661A (zh) * 2021-04-28 2021-08-24 江汉大学 一种清洁检测系统及其控制方法和使用方法

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2001025387A (ja) * 1999-05-12 2001-01-30 Astec:Kk クリーンベンチ
JP2016165249A (ja) * 2015-03-10 2016-09-15 株式会社日立産機システム クリーンエア装置
JP2018011544A (ja) * 2016-07-20 2018-01-25 パナソニックＩｐマネジメント株式会社 微生物検出方法及び微生物検出装置

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2001025387A (ja) * 1999-05-12 2001-01-30 Astec:Kk クリーンベンチ
JP2016165249A (ja) * 2015-03-10 2016-09-15 株式会社日立産機システム クリーンエア装置
JP2018011544A (ja) * 2016-07-20 2018-01-25 パナソニックＩｐマネジメント株式会社 微生物検出方法及び微生物検出装置

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
HASEGAWA, NORIO ET AL.: "Instantaneous bioaerosol detection technology and its application", AZBIL TECHNICAL REVIEW, 1 December 2009 (2009-12-01), pages 2 - 7 *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113295661A (zh) * 2021-04-28 2021-08-24 江汉大学 一种清洁检测系统及其控制方法和使用方法

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