WO2019169807A1 - Method for flexible sterilization of spray-dried-and-prepared barley grass powder and effective storage of nutrition component - Google Patents

Method for flexible sterilization of spray-dried-and-prepared barley grass powder and effective storage of nutrition component Download PDF

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Publication number
WO2019169807A1
WO2019169807A1 PCT/CN2018/093125 CN2018093125W WO2019169807A1 WO 2019169807 A1 WO2019169807 A1 WO 2019169807A1 CN 2018093125 W CN2018093125 W CN 2018093125W WO 2019169807 A1 WO2019169807 A1 WO 2019169807A1
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Prior art keywords
sterilization
ozone
wheat
rpm
powder
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PCT/CN2018/093125
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French (fr)
Chinese (zh)
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张慜
曹笑皇
陈龙海
周乐群
黄少波
杨再兴
李冰岩
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江南大学
宁波海通食品科技有限公司
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Publication of WO2019169807A1 publication Critical patent/WO2019169807A1/en

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    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L3/00—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs
    • A23L3/005—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs by heating using irradiation or electric treatment
    • A23L3/01—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs by heating using irradiation or electric treatment using microwaves or dielectric heating
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23B—PRESERVING, e.g. BY CANNING, MEAT, FISH, EGGS, FRUIT, VEGETABLES, EDIBLE SEEDS; CHEMICAL RIPENING OF FRUIT OR VEGETABLES; THE PRESERVED, RIPENED, OR CANNED PRODUCTS
    • A23B9/00—Preservation of edible seeds, e.g. cereals
    • A23B9/08—Drying; Subsequent reconstitution
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L3/00—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs
    • A23L3/26—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs by irradiation without heating
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L3/00—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs
    • A23L3/34—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs by treatment with chemicals
    • A23L3/3409—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs by treatment with chemicals in the form of gases, e.g. fumigation; Compositions or apparatus therefor
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L3/00—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs
    • A23L3/34—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs by treatment with chemicals
    • A23L3/3409—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs by treatment with chemicals in the form of gases, e.g. fumigation; Compositions or apparatus therefor
    • A23L3/3445—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs by treatment with chemicals in the form of gases, e.g. fumigation; Compositions or apparatus therefor in a controlled atmosphere comprising other gases in addition to CO2, N2, O2 or H2O
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L3/00—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs
    • A23L3/40—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs by drying or kilning; Subsequent reconstitution
    • A23L3/46—Spray-drying
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A23L33/105—Plant extracts, their artificial duplicates or their derivatives
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L7/00—Cereal-derived products; Malt products; Preparation or treatment thereof
    • A23L7/10—Cereal-derived products
    • A23L7/198—Dry unshaped finely divided cereal products, not provided for in groups A23L7/117 - A23L7/196 and A23L29/00, e.g. meal, flour, powder, dried cereal creams or extracts
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs

Definitions

  • the invention relates to a method for flexible sterilization and effective preservation of nutrient components of spray drying conditioning wheat seed powder, in particular to a wheat seed powder obtained by spray drying of young barley leaf juice as a main raw material, and anti-oxidation substance is added after ozone sterilization by ozone synergy.
  • the method of protecting the superoxide-oxidizing enzyme from the red peony polyphenol and introducing the instant-conditioning and low-temperature storage of the instant wheat seedling powder health food under the inert gas belongs to the technical field of processing health food.
  • Wheat seedling powder is processed from the stems and leaves of wheat seedlings and is rich in plant brass, active enzymes SOD, chlorophyll, protein, amino acids, vitamins and minerals.
  • This kind of compound nutrition product can enhance the immune function enhancer, wherein SOD, flavonoids and chlorophyll are key components of wheat seedling powder, and play a major role in antioxidant.
  • the juice-dried, spray-dried wheat seed powder is small, more easily absorbed by the human body, and the taste is more delicate; nano-zinc combined with radio frequency sterilization reduces the intensity of sterilization to a greater extent, and the content of SOD and chlorophyll flavonoids is high, which is more conducive to improving the human body. Immunity.
  • Spray drying is a traditional drying technology. It has the greatest protection against SOD activity through its embedding and red bay polyphenols, and improves the stability of SOD vitality.
  • Ozone synergistic radiofrequency pasteurization has high efficiency and energy-saving sterilization.
  • Sexuality greatly reduces the time and temperature of sterilization, improves the quality of wheat seedling powder, and maintains the activity of superoxide dismutase. Removal of oxygen and nitrogen storage in the dark, protects the superoxide dismutase and flavonoids from damage, comprehensively stabilizes the antioxidant properties of the wheat seed powder, and the product is balanced.
  • Zhang Gao Yujing and Chen Weiping disclose a flexible sterilization method for promoting the preservation of fish paste flavor.
  • 80-100 nm nano-silver and 55-80 nm nano-zinc were added and sterilized at 90 ° C for 10 min, so that the shelf life reached 12 months.
  • the amount of nano zinc added is 0.1-0.15 mg/kg
  • the amount of nano silver added is 0.10-0.13 mg/kg.
  • the difference between the present invention and its sterilization retention is a flexible sterilization method using ozone synergistic radio frequency sterilization, and the product is stored by using a combination inert gas.
  • Zhang , Xu Cheng, et al. discloses a method for determining the shelf life of flexible bactericidal fruit and vegetable dishes by low field nuclear magnetic combined with electronic nose.
  • radio frequency sterilization technology sterilization, combined with nuclear magnetic moisture measurement to establish a kinetic model, that is, fruit and vegetable dishes shelf life model.
  • the patent is available in the range of 4 ° C to 28 ° C, which can quickly predict the shelf life and facilitate production.
  • the difference between the present invention is that the distance between the RF plates is 14, 13 and 11 cm; the temperature of the auxiliary hot gas is 55 °C.
  • Shao Chengbin et al. (Chinese Patent Application No. 200410040600.7) discloses a wheat seed powder for controlling insulin resistance syndrome and a preparation process thereof. After the washed and sterilized wheat seed juice is filtered, the tea polyphenol and the straw ash extract are added to the filtrate, and the filter residue is extracted twice with ethanol to obtain an extract filtrate, and then all the filtrates are combined, and the precipitate is separated and settled at a low temperature to obtain a wheat. Green granule paste, finally added Huang's dip, licorice extract, ⁇ -cyclodextrin and pueraria powder, vacuum dried, spray dried or freeze-dried, and then ground to obtain the product.
  • Li Ji discloses a preparation process of a wintering barley prebiotic composite powder.
  • the wintering barley leaves are pulverized, added with milk powder to soak, and then added to the white simmer to be beaten, and the juice after beating is spray-dried, and the obtained product is combined with prebiotics and maltose.
  • the prebiotic compound powder has the effects of inhibiting harmful bacteria, balance of colonic colonies of the heavens, and regulating blood lipids.
  • Gu Shouzhi et al. discloses a chlorophyll formulation and a preparation process thereof. 82% ⁇ 95% of wheat chlorophyll powder, 5% ⁇ 18% edible cactus extract is mixed and tableted, wherein the chlorophyll powder is fresh barley leaf extract.
  • Gu Zhenxin et al. discloses a compound type chlorophyll solid beverage and a production process thereof.
  • the fresh wheat seedling juice of bio-green protection is extracted with Flammulina velutipes extract, and the chlorophyll powder obtained by vacuum freeze-drying is used as the main raw material, and the wheat seed slag powder, spirulina powder, xanthan gum, sorbitol, sodium alginate, malt are added.
  • Zhang And Xu Cheng et al disclose a flexible sterilization method for prolonging the low-quality shelf life of Gexianmi.
  • the invention adopts low acid fresh-keeping liquid and radio-hot air combined sterilization, and after vacuum packaging, the shelf life can be extended for more than 9 months, the hardness is above 350g, and the color b* is above 2.
  • the total number of colonies of the finished product was ⁇ 1000 CFU/g, and pathogenic bacteria such as Salmonella were not detected.
  • the difference between the present invention is that the synergistic effect of ozone treatment is increased on the basis of the combination of radio frequency and hot air.
  • Zhang And Xu Cheng et al. discloses a flexible sterilization method for small-packaged fresh-cut vegetables.
  • the vegetables were soaked in 100mg/L sodium hypochlorite for 3-5min, and the nano-zinc was added and treated by radio frequency combined with hot air.
  • the shelf life was 9 months.
  • the difference between the present invention is that the product is subjected to ozone synergistic radio frequency sterilization in a dry state; the distance between the plates of the radio frequency is between 11 and 14 cm.
  • Zhou Jianhe et al. discloses a method for preparing a chewable tablet and a medium-short wave infrared drying method in which a wheat seed powder and an edible mushroom powder are combined.
  • the wheat seedling micropowder was obtained by superfine pulverization of barley seedling powder, and the wheat seedling powder and edible fungus fine powder were used as the main raw materials.
  • the wet granulation, demoulding, medium-short wave infrared drying, film, and medium-short wave infrared drying were used to prepare the wheat seedlings.
  • a chewable tablet of powder and edible mushroom powder were used to prepare the wheat seedlings.
  • Zhang And Wang Lin et al. discloses a method for flexible sterilization of RF hot air combined with fish gill products.
  • the surimi is placed on the polyether imine medium block in the RF working chamber, and directly combined with radio-hot air sterilization.
  • the obtained surimi can better maintain the flavor, texture, color and nutrition, and the shelf life of the normal temperature can reach more than 9 months, and the market prospect is broad.
  • Zhang , Yang Fanli, Yang Zhaohui discloses a method for prolonging the shelf life of Babao duck by using combined flexible sterilization.
  • the invention adds a clove/cinnamon/onion composite extract during the preparation process of Babao duck stuffing.
  • Nano-preservative after cooking, vacuum-packing Babao duck with retort pouch, placing the packaged Babao duck in the RF working chamber for heat sterilization, then performing short-time high-temperature and high-pressure sterilization and rapid cooling.
  • the quality preservation rate of the color and flavor of the eight treasure duck body and stuffing heart reached 90% and 88%, respectively, and achieved commercial sterility without significantly adding artificial preservatives, significantly extending the product.
  • the present invention differs from the above invention in that ozone is used in conjunction with radio frequency sterilization and inert gas storage.
  • Zhang Wang Yuyu, Sun Lei, Wang Xianglian, Liu Chunquan (application number: 201710098334.0) discloses a method for prolonging the shelf life of salted geese by pasteurization combined with nano zinc oxide bacteriostatic and radio frequency sterilization, and the goose meat of the invention is subjected to low temperature pasteurization. Before vacuum packaging, add nano-zinc oxide to inhibit bacteria, vacuum-package and then radio-frequency sterilization.
  • the method avoids the problem of high quality destruction of goose meat by using high temperature and high pressure sterilization, and can prolong the shelf life of 25 ° C of salt water goose to 5-6 months, and the shelf life of 4 ° C to 11-12 months, while maximizing The quality of the goose is guaranteed, and the preservation rate of flavor, color, taste and texture is about 93%.
  • the present invention differs from the invention in that ozone is synergistically sterilized, and the hot gas stream and the inert gas are stored at a low temperature.
  • the above mainly uses the combined synergistic flexible sterilization technology to treat the wheat seedling powder, and the patent protects the quality of the wheat seedling powder and the activity of superoxide-salting enzyme (SOD) by means of ozone synergistic sterilization and inert gas protection.
  • SOD superoxide-salting enzyme
  • the purpose of the invention is to reduce the sterilization intensity of the wheat seed powder, improve the nutritional value of the quality, maintain the activity of the SOD, use the ozone synergistic radio frequency sterilization technology, add the SOD active protective agent bayberry polyphenol, protect the group by inert gas, avoid the light and store at low temperature.
  • Instantly conditioned green wheat flour product that maintains superoxide dismutase activity.
  • the invention relates to a method for flexible sterilization of spray-dried conditioning wheat seedling powder and effective preservation of nutrient components, characterized in that the method comprises impurity removal, assembly, loading, ozone and variable speed stirring, two-band radio frequency sterilization, natural cooling, and dispensing , inert gas protection, sealing and protection from light and low temperature storage, the specific steps are as follows:
  • the wheat seedling powder is spread on the feeding tray of the vibrating screen; the wheat seedling powder enters the sieve through a magnetic adsorption channel having a diameter of 3 cm, and the sieve mesh is shaken at a frequency of 30 Hz for 1-2 min to adsorb and remove the wheat seed powder.
  • the metal impurities in the subsequent collection is a circle having a diameter of 2 m, and the opening ratio of the steel mesh on the vibrating screen is 0.8, and the pore diameter is 1 mm;
  • step (1) Add the antioxidant substance, bayberry polyphenols and nutrients to the wheat seed powder collected in step (1).
  • the content of the antioxidant substance, bayberry polyphenol, is 0.5-1.5%; after mixing, mix with a blender, first in the Stirring at rpm for 1-2 min, then stirring at 300 rpm for 10-20 s; wherein, the mixer power is 700 W, the rotational speed is 10-500 rpm;
  • the wheat seedling powder obtained in the step (2) is placed in a container, the container mouth is plugged with a two-hole polyvinyl fluoride stopper; wherein the container has a diameter of 30 cm and a height of 8 cm;
  • Two-band radio frequency sterilization the two-band radio frequency sterilization treatment of the step (4) ozone-treated wheat seed powder is first sterilized at 27.12 MHz for 3-10 min, and then sterilized at 40.68 MHz for 2-15 min; wherein, the radio frequency plate The spacing is 12-14cm, and the auxiliary airflow heat temperature is 45-55 ° C;
  • Inert gas protection slowly fill the composite inert gas from the bottom of the foil bag, and replace the oxygen in the bag with the composite inert gas;
  • thermoplastic package is sealed
  • step (4) and step (5) are divided into three cases, specifically:
  • step (5) when the RF plate spacing is 14cm, first sterilize at 27.12MHz for 5min, then sterilize at 48.68MHz for 10min, and the auxiliary airflow heat temperature is 55 °C; step (4) In the ozone-filling condition, the wheat seedling powder is first stirred at a stirring speed of 10-30 rpm for 20-60 s, then stirred at a stirring speed of 20-50 rpm for 20-60 s, the ozone concentration is 20 ppm, and the aeration flow rate is 1-3 ml/s. ;
  • step (5) when the RF plate spacing is 13cm, first sterilize at 27.12MHz for 5min, then sterilize at 48.68MHz for 8min, and the auxiliary airflow heat temperature is 55 °C; step (4) The wheat seedling powder is stirred under the condition of ozone charging for 20-60 s at a stirring speed of 10-30 rpm, then stirred at a stirring speed of 20-50 rpm for 20-60 s, the ozone concentration is 15 ppm, and the aeration speed is 1-3 ml/s;
  • step (5) when the RF plate spacing is 12cm, first sterilize at 27.12MHz for 5min, then sterilize at 48.68Mhz for 5min, and the auxiliary hot gas heat temperature is 55°C; step (4) In the ozone-filling condition, the wheat seedling powder is first stirred at a stirring speed of 10-30 rpm for 20-60 s, then stirred at a stirring speed of 20-50 rpm for 20-60 s, the ozone concentration is 10 ppm, and the gas flow rate is 1-3 ml/ s;
  • the chlorophyll content, total flavonoid content and SOD activity of the obtained wheat seedling powder are specifically as follows: the wheat seed powder obtained by using large distance ozone to assist radiofrequency sterilization: the chlorophyll content ranges from 500 to 550 mg/100 g, and the total flavonoid content ranges from 450 to 480 mg/ 100g, SOD activity 300-380U/mg, vitamin C 0.5%, the preservation rate was higher than 93% compared with before sterilization.
  • chlorophyll content range 500 ⁇ 540mg/100g
  • total flavonoid content range 470 ⁇ 510mg/100g
  • SOD activity 290-380U/mg SOD activity 290-380U/mg
  • vitamin C 1% SOD activity 290-380U/mg
  • the preservation rate is higher than 90%.
  • the wheat seedling powder prepared by using small distance ozone to assist radiofrequency sterilization chlorophyll content range 500 ⁇ 530mg/100g, flavonoid content range 450 ⁇ 510mg/100g, SOD activity 250-380U/mg, vitamin C 2%, and pre-sterilization phase
  • chlorophyll content range 500 ⁇ 530mg/100g chlorophyll content range 500 ⁇ 530mg/100g
  • flavonoid content range 450 ⁇ 510mg/100g
  • SOD activity 250-380U/mg SOD activity 250-380U/mg
  • vitamin C 2% pre-sterilization phase
  • the retention rate is higher than 90%.
  • the composite inert gas formulation of step (7) is: by volume ratio:
  • the wheat seedling powder is spray-dried, the drying time is short, the nutrient is high, and the functional component loss is small.
  • the antioxidant substance Yangmei polyphenol is added, which maintains the SOD activity, improves the functional effect of the product, and improves the immunity of the human body.
  • Example 1 Sterilization and preservation method for large distance ozone assisted radio frequency sterilization and high nitrogen inert gas protection
  • the chlorophyll content ranged from 500 to 550 mg/100 g
  • the total flavonoid content ranged from 450 to 480 mg/100 g
  • the SOD activity was 370 U/mg
  • the vitamin C was 0.5%.
  • the antioxidant activity of chlorophyll, flavonoids and superoxide-enriched enzyme was preserved compared with that before sterilization. The rate is higher than 93%.
  • This product is green in color, has strong wheat scent, uniform particles, dissolves rapidly in water at 30 ° C for 20 s without any precipitation, and the wort soup is shiny.
  • auxiliary hot air temperature 50 ° C, radio frequency sterilization at 27.12 MHz for 5 min, then radio frequency sterilization for 5 min at 40.78 MHz; radio frequency plate spacing 13 cm, power 6 kW; natural cooling in the drying chamber, sub-packaged in tin foil bags; sealed from tin foil
  • 100g of each bag is stored in the dark and protected from light for a period of more than 12 months.
  • the chlorophyll content ranged from 500 to 540 mg/100 g
  • the total flavonoid content ranged from 470 to 510 mg/100 g
  • the SOD activity was 350 U/mg
  • the vitamin C was 1%.
  • the antioxidant activity retention rates of chlorophyll, flavonoids and superoxide-enriched enzymes were all higher than 90%; the product could be dissolved quickly (10s) in warm water at 50 °C without agglomeration, no obvious sinking phenomenon.
  • Example 3 Sterilization and preservation method for small distance ozone assisted radio frequency sterilization and high nitrogen inert gas protection
  • 1.5 kg of spray-dried wheat seedling powder was taken and spread on a magnetic vibrating sieve machine, and shaken at a vibration frequency of 30 Hz for 2 min to collect the wheat seed powder.
  • the content of the anti-oxidant arbutin was 1.5%; the mixture was uniformly mixed with a mixer; the stirring time was 1.5 rpm at 100 rpm, and the mixture was stirred at 300 rpm for 20 s.
  • the mixture was placed in a sterilizing glass container at room temperature of 25 ° C, and a polyvinyl fluoride stopper was placed. Connect the ozone generator and the sterilization plug with a leather tube.
  • the diameter of the skin tube is 0.5cm and the length is 1m.
  • the ozone is added to the mixture while stirring; the ozone concentration is 10ppm, the inlet speed is 3ml/s, the stirring speed is 20rpm, the stirring time is 30s, then the stirring speed is 40rpm. Stir for 30 s.
  • the sealing RF sterilization set the auxiliary hot air temperature to 55 ° C, radio frequency sterilization at 27.12 MHz for 4 min, then radio sterilization at 40.78 MHz for 4 min; RF plate spacing 12 cm, power 6 kW;
  • 100g of each bag is stored in the dark and protected from light for a period of more than 12 months.
  • the chlorophyll content ranged from 500 to 530 mg/100 g
  • the flavonoid content ranged from 490 to 510 mg/100 g
  • the SOD activity was 330 U/mg
  • the vitamin C was 2%.
  • the antioxidant activity retention rate of chlorophyll, flavonoids and superoxide-enriched enzyme was compared with that before sterilization. Both are higher than 90%; the product color is green, the wheat aroma is strong, the taste is lubricated and cool, at 30 ° C, 10s can be completely dissolved, no precipitation.

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  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
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  • Polymers & Plastics (AREA)
  • Health & Medical Sciences (AREA)
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  • Wood Science & Technology (AREA)
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  • Coloring Foods And Improving Nutritive Qualities (AREA)
  • Food Preservation Except Freezing, Refrigeration, And Drying (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)

Abstract

Provided is a method for flexible sterilization of spray-dried-and-prepared barley grass powder and effective storage of a nutrition component, wherein the barley grass powder obtained from spray drying is the main ingredient, the method comprising: removal of impurities, composition, loading, injection of ozone and variable speed stirring, two-band radio frequency sterilization, natural cooling, packing, inertial gas protection, sealing, storage at low temperature away from light, and other steps. the combination of the radio frequency sterilization technology and the two-band radio frequency sterilization technology by employing ozone enables short sterilization time and little loss of nutrients and functional components; the adding of antioxidant bayberry polyphenol keeps the high activity of barley grass powder superoxide dismutase, chlorophyll, and flavone, etc.; the injection of mixed inert gas avoids oxidation deactivation of nutrient components such as superoxide dismutase, barley green, and flavone, thus keeping the antioxidation activity retention rate above 90% after sterilization.

Description

一种喷雾干燥调理麦苗粉柔性杀菌及营养组分有效保存的方法Method for flexible sterilization and effective preservation of nutrient components of spray drying conditioning wheat seed powder 技术领域Technical field
本发明涉及一种喷雾干燥调理麦苗粉柔性杀菌及营养组分有效保存的方法，具体涉及一种以幼年大麦叶汁喷雾干燥所得麦苗粉为主要原料，通过臭氧协同射频杀菌后，添加抗氧化物质杨梅多酚保护超氧化酶，并通入惰性气体下，避光低温贮藏的速溶调理麦苗粉保健食品的方法，属于保健食品的加工技术领域。The invention relates to a method for flexible sterilization and effective preservation of nutrient components of spray drying conditioning wheat seed powder, in particular to a wheat seed powder obtained by spray drying of young barley leaf juice as a main raw material, and anti-oxidation substance is added after ozone sterilization by ozone synergy. The method of protecting the superoxide-oxidizing enzyme from the red peony polyphenol and introducing the instant-conditioning and low-temperature storage of the instant wheat seedling powder health food under the inert gas belongs to the technical field of processing health food.
背景技术Background technique
麦苗粉是以麦苗嫩茎叶加工而成，富含植物黄铜、活性酶SOD、叶绿素、蛋白质、氨基酸、维生素和矿物质等。这种复合营养产品是能提高免疫功能增强剂，其中SOD、黄酮、叶绿素是麦苗粉关键组分，在抗氧化方面起到主要作用。通过榨汁、喷雾干燥的麦苗粉颗粒小，更容易被人体吸收，口感更加细腻；纳米锌联合射频杀菌，更大程度降低了杀菌的强度，产品SOD及叶绿素黄酮的含量高，更利于提高人体免疫力。Wheat seedling powder is processed from the stems and leaves of wheat seedlings and is rich in plant brass, active enzymes SOD, chlorophyll, protein, amino acids, vitamins and minerals. This kind of compound nutrition product can enhance the immune function enhancer, wherein SOD, flavonoids and chlorophyll are key components of wheat seedling powder, and play a major role in antioxidant. The juice-dried, spray-dried wheat seed powder is small, more easily absorbed by the human body, and the taste is more delicate; nano-zinc combined with radio frequency sterilization reduces the intensity of sterilization to a greater extent, and the content of SOD and chlorophyll flavonoids is high, which is more conducive to improving the human body. Immunity.
喷雾干燥是传统干燥技术，通过其包埋作用及杨梅多酚发挥了对SOD活力最大程度的保护，提高了SOD的活力的稳定性；臭氧协同射频巴氏杀菌，具有杀菌的高效性和节能型性，大大降低了杀菌的时间及温度，提高麦苗粉的品质，保持超氧歧化酶的活力。除去氧气充入氮气避光低温贮藏，有助于超氧歧化酶及黄酮叶绿素免受破坏、综合稳定了麦苗粉的抗氧化性，所得产品营养均衡。Spray drying is a traditional drying technology. It has the greatest protection against SOD activity through its embedding and red bay polyphenols, and improves the stability of SOD vitality. Ozone synergistic radiofrequency pasteurization has high efficiency and energy-saving sterilization. Sexuality greatly reduces the time and temperature of sterilization, improves the quality of wheat seedling powder, and maintains the activity of superoxide dismutase. Removal of oxygen and nitrogen storage in the dark, protects the superoxide dismutase and flavonoids from damage, comprehensively stabilizes the antioxidant properties of the wheat seed powder, and the product is balanced.
张
Figure 615c_1
、高玉静和陈卫平（专利申请号201110395050.0）公开了一种促进鱼粒酱风味保藏的柔性杀菌方法。添加80-100nm的纳米银和55-80nm纳米锌，在90℃下杀菌10min，使得保质期达到12月。纳米锌的添加量为0.1-0.15mg/kg，纳米银添加量为0.10-0.13mg/kg。本发明与其杀菌保留不同之处为采用臭氧协同射频杀菌的柔性杀菌方法，并且利用组合惰性气体对产品贮藏。Zhang
Figure 615c_1
Gao Yujing and Chen Weiping (Patent Application No. 201110395050.0) disclose a flexible sterilization method for promoting the preservation of fish paste flavor. 80-100 nm nano-silver and 55-80 nm nano-zinc were added and sterilized at 90 ° C for 10 min, so that the shelf life reached 12 months. The amount of nano zinc added is 0.1-0.15 mg/kg, and the amount of nano silver added is 0.10-0.13 mg/kg. The difference between the present invention and its sterilization retention is a flexible sterilization method using ozone synergistic radio frequency sterilization, and the product is stored by using a combination inert gas.
陈石良（专利申请号201610749082.9）公开了一种抗氧化SOD口腔喷雾剂及制备方法。通过SOD、维生素C、绿茶提取物和海藻糖相互保护，相互协同，设下粘膜直接吸收，服用方便，大大提高SOD的利用效率。口腔喷剂含SOD1.0-2.0%，维生素C 1.0-5.0%，海藻糖1.0-3.5%，绿茶提取物0.2-1.0%。本发明与其不同之处为采用抗氧化物质和惰性气体对麦苗粉保护。Chen Shiliang (Patent Application No. 201610749082.9) discloses an antioxidant SOD oral spray and a preparation method thereof. Through SOD, vitamin C, green tea extract and trehalose mutual protection, synergy, direct absorption of the mucous membrane, easy to take, greatly improve the utilization efficiency of SOD. Oral spray contains SOD 1.0-2.0%, vitamin C 1.0-5.0%, trehalose 1.0-3.5%, green tea extract 0.2-1.0%. The present invention differs from the use of antioxidants and inert gases to protect wheat seedlings.
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Figure 615c_1
、徐继承等（专利申请号201610821923.2）公开了一种低场核磁结合电子鼻确定柔性杀菌调理果蔬菜肴保质期的方法。利用射频杀菌技术杀菌，结合核磁水分测量建立动力学模型即果蔬菜肴保质期模型。该专利在4℃-28℃范围内，可以快速预测出货架期，为生产提供便利。射频杀菌条件，极板间距12cm，功率6kW。本发明与其不同之处为射频极板间距为14、13、11cm；使用辅助热气流温度为55℃。Zhang
Figure 615c_1
, Xu Cheng, et al. (Patent Application No. 201610821923.2) discloses a method for determining the shelf life of flexible bactericidal fruit and vegetable dishes by low field nuclear magnetic combined with electronic nose. Using radio frequency sterilization technology sterilization, combined with nuclear magnetic moisture measurement to establish a kinetic model, that is, fruit and vegetable dishes shelf life model. The patent is available in the range of 4 ° C to 28 ° C, which can quickly predict the shelf life and facilitate production. Radio frequency sterilization conditions, plate spacing 12cm, power 6kW. The difference between the present invention is that the distance between the RF plates is 14, 13 and 11 cm; the temperature of the auxiliary hot gas is 55 °C.
邵承斌等(中国专利申请号200410040600.7)公开了一种用于防治胰岛素抵抗综合症的麦苗粉及其制备工艺。将洗净消毒的麦苗榨汁过滤后，在滤液中添加茶多酚和稻草灰提取液，滤渣用乙醇萃取两次获得萃取滤液，再将所有滤液合并，在低温下静置沉降分离制得麦绿素膏，最后添加黄氏浸、甘草浸膏、β-环糊精和葛根粉，经过真空干燥、喷雾干燥或冷冻干燥，再研磨获得产品。Shao Chengbin et al. (Chinese Patent Application No. 200410040600.7) discloses a wheat seed powder for controlling insulin resistance syndrome and a preparation process thereof. After the washed and sterilized wheat seed juice is filtered, the tea polyphenol and the straw ash extract are added to the filtrate, and the filter residue is extracted twice with ethanol to obtain an extract filtrate, and then all the filtrates are combined, and the precipitate is separated and settled at a low temperature to obtain a wheat. Green granule paste, finally added Huang's dip, licorice extract, β-cyclodextrin and pueraria powder, vacuum dried, spray dried or freeze-dried, and then ground to obtain the product.
李季（专利申请号201611084065.4）公开了一种越冬大麦益生元复合粉剂的制备工艺。将越冬大麦嫩叶粉碎后加入奶粉浸泡，然后加入白芷浸泡后打浆，打浆后的汁液喷雾干燥，所得产品与益生元、麦芽糖等组方。本益生元复合粉具有抑制有害菌，天界肠道菌落平衡，调节血脂等功效。Li Ji (Patent Application No. 201611084065.4) discloses a preparation process of a wintering barley prebiotic composite powder. The wintering barley leaves are pulverized, added with milk powder to soak, and then added to the white simmer to be beaten, and the juice after beating is spray-dried, and the obtained product is combined with prebiotics and maltose. The prebiotic compound powder has the effects of inhibiting harmful bacteria, balance of colonic colonies of the heavens, and regulating blood lipids.
顾守智等（中国专利申请号200810060880.6）公开了一种麦绿素配方及其制剂工艺。将82%~95%麦绿素粉，5%~18%食用仙人掌提取物混合再经压片制成片剂，其中麦绿素粉是新鲜大麦嫩叶提取物。Gu Shouzhi et al. (Chinese Patent Application No. 200810060880.6) discloses a chlorophyll formulation and a preparation process thereof. 82%~95% of wheat chlorophyll powder, 5%~18% edible cactus extract is mixed and tableted, wherein the chlorophyll powder is fresh barley leaf extract.
顾振新等（中国专利申请号201110446292.8）公开了一种复配型麦绿素固体饮料及其生产工艺。以金针菇提取液进行生物护绿的新鲜麦苗汁液，经真空冷冻干燥制得的麦绿素粉为主要原料，添加麦苗渣粉、螺旋藻粉、黄原胶、山梨糖醇、海藻酸钠、麦芽糊精、羧甲基纤维素钠，经灭菌和包装工序后制得。Gu Zhenxin et al. (Chinese Patent Application No. 201110446292.8) discloses a compound type chlorophyll solid beverage and a production process thereof. The fresh wheat seedling juice of bio-green protection is extracted with Flammulina velutipes extract, and the chlorophyll powder obtained by vacuum freeze-drying is used as the main raw material, and the wheat seed slag powder, spirulina powder, xanthan gum, sorbitol, sodium alginate, malt are added. Dextrin, sodium carboxymethyl cellulose, obtained after sterilization and packaging.
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Figure 615c_1
和徐继承等（201610824919.5）公开了一种延长葛仙米低温优质货架期的柔性杀菌方法。本发明采用低酸保鲜液和射频-热风联合杀菌，真空封装后，可延长货架期9个月以上，硬度在350g以上，色泽b*在2以上。成品总菌落数≤1000CFU/g，沙门氏菌等致病菌未检出。本发明与其不同之处为在射频-热风联合的基础上增加了臭氧处理的杀菌协同效应。Zhang
Figure 615c_1
And Xu Cheng et al (201610824919.5) disclose a flexible sterilization method for prolonging the low-quality shelf life of Gexianmi. The invention adopts low acid fresh-keeping liquid and radio-hot air combined sterilization, and after vacuum packaging, the shelf life can be extended for more than 9 months, the hardness is above 350g, and the color b* is above 2. The total number of colonies of the finished product was ≤1000 CFU/g, and pathogenic bacteria such as Salmonella were not detected. The difference between the present invention is that the synergistic effect of ozone treatment is increased on the basis of the combination of radio frequency and hot air.
张秋英等人（2009）研究了麦苗营养对人体健康方面的作用，结果显示，麦苗粉能有效的改善睡眠，提高免疫力，修复记忆力，促进伤口愈合，增强体力，缓解人体各种不适反应，增进机体排毒，延缓人体衰老。尤其对于年龄较大、亚健康体质或身体健康出现问题的人群，效果更加明显。并且长期坚持每天口服少量麦苗粉，发现其对人体产生毒副作用。Zhang Qiuying et al. (2009) studied the effects of wheat seedling nutrition on human health. The results showed that wheat seedling powder can effectively improve sleep, improve immunity, repair memory, promote wound healing, enhance physical strength, and alleviate various uncomfortable reactions. Improve the body's detoxification and delay the aging of the human body. Especially for people with older, sub-healthy physical or physical health problems, the effect is more obvious. And long-term adherence to a small amount of wheat powder per day, found that it has toxic side effects on the human body.
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Figure 615c_1
和徐继承等（专利申请号20160010015.5）公开了一种小包装根茎类鲜切蔬菜的柔性杀菌方法。蔬菜在100mg/L次氯酸钠浸泡3-5min，加入纳米锌，通过射频联合热风处理，货架期达到9个月。纳米锌质量浓度0.1%，SO6B型射频杀菌设备，极板16cm，功率6kW。本发明与其不同之处为产品在干燥状态下进行臭氧协同射频杀菌；射频的极板间距为11-14cm之间。Zhang
Figure 615c_1
And Xu Cheng et al. (Patent Application No. 20160010015.5) discloses a flexible sterilization method for small-packaged fresh-cut vegetables. The vegetables were soaked in 100mg/L sodium hypochlorite for 3-5min, and the nano-zinc was added and treated by radio frequency combined with hot air. The shelf life was 9 months. Nano zinc concentration 0.1%, SO6B type RF sterilization equipment, plate 16cm, power 6kW. The difference between the present invention is that the product is subjected to ozone synergistic radio frequency sterilization in a dry state; the distance between the plates of the radio frequency is between 11 and 14 cm.
周建贺等（中国专利申请号201210397017）公开了一种麦苗粉与食用菌粉复合的咀嚼片制备及中短波红外干燥的方法。以大麦苗粗粉经超微粉碎得麦苗微粉，以麦苗微粉和食用菌细粉为主要原料，经湿法制粒、脱模、中短波红外干燥、被膜、再中短波红外干燥，包装制得麦苗粉与食用菌粉复合的咀嚼片。Zhou Jianhe et al. (Chinese Patent Application No. 201210397017) discloses a method for preparing a chewable tablet and a medium-short wave infrared drying method in which a wheat seed powder and an edible mushroom powder are combined. The wheat seedling micropowder was obtained by superfine pulverization of barley seedling powder, and the wheat seedling powder and edible fungus fine powder were used as the main raw materials. The wet granulation, demoulding, medium-short wave infrared drying, film, and medium-short wave infrared drying were used to prepare the wheat seedlings. A chewable tablet of powder and edible mushroom powder.
张
Figure 615c_1
和王琳等（专利申请号201610009458.2）公开了一种射频热风联合对食鱼糜制品的柔性杀菌方法。将鱼糜放置射频工作腔内的聚醚亚胺介质块上，直接射频-热风联合杀菌。所得鱼糜能够较好的保持风味、质构、色泽和营养，常温保质期可达9个月以上，市场前景广阔。Zhang
Figure 615c_1
And Wang Lin et al. (Patent Application No. 201610009458.2) discloses a method for flexible sterilization of RF hot air combined with fish gill products. The surimi is placed on the polyether imine medium block in the RF working chamber, and directly combined with radio-hot air sterilization. The obtained surimi can better maintain the flavor, texture, color and nutrition, and the shelf life of the normal temperature can reach more than 9 months, and the market prospect is broad.
张
Figure 615c_1
、杨帆里、杨朝晖（申请号：201710028400.7）公开一种利用联合柔性杀菌延长调理八宝鸭货架期的方法，本发明在八宝鸭馅心制作过程中添加含丁香/肉桂/洋葱复合提取物的纳米防腐剂，熟制后再使用蒸煮袋真空包装八宝鸭，将包装八宝鸭置于射频工作腔内加热杀菌，然后进行短时高温高压杀菌，快速冷却。经柔性杀菌后八宝鸭鸭体及馅心的色香味形的品质保存率分别达到90%、88%，并在不添加人工防腐剂的前提下，达到了商业无菌，显著延长了产品的优质货架期，夏季室温（25‑35℃）优质货架期可达到60d，低温（4℃）优质货架期可达到180d。本发明与上述发明区别是采用臭氧协同射频杀菌，和惰性气体贮藏。Zhang
Figure 615c_1
, Yang Fanli, Yang Zhaohui (Application No.: 201710028400.7) discloses a method for prolonging the shelf life of Babao duck by using combined flexible sterilization. The invention adds a clove/cinnamon/onion composite extract during the preparation process of Babao duck stuffing. Nano-preservative, after cooking, vacuum-packing Babao duck with retort pouch, placing the packaged Babao duck in the RF working chamber for heat sterilization, then performing short-time high-temperature and high-pressure sterilization and rapid cooling. After the flexible sterilization, the quality preservation rate of the color and flavor of the eight treasure duck body and stuffing heart reached 90% and 88%, respectively, and achieved commercial sterility without significantly adding artificial preservatives, significantly extending the product. High quality shelf life, high temperature shelf life of 60d at room temperature (25‐35°C) in summer, and high shelf life of 180d at low temperature (4°C). The present invention differs from the above invention in that ozone is used in conjunction with radio frequency sterilization and inert gas storage.
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Figure 615c_1
、王婧宇、孙磊、王向连、刘春泉（申请号：201710098334.0）公开一种采用巴氏杀菌结合纳米氧化锌抑菌和射频杀菌延长盐水鹅保质期的方法，本发明鹅肉经过低温巴氏杀菌，在真空包装前，先添加纳米氧化锌抑菌，真空包装后再进行射频杀菌。该方法又避免了使用高温高压杀菌对鹅肉品质破坏较大的问题，可以延长盐水鹅的25℃室温保质期至5‑6个月，4℃低温保质期至11‑12个月，同时最大限度的保证了鹅肉的品质，使风味、色泽、味道、质地的保存率分别达到93%左右。本发明与该发明的区别在于臭氧协同杀菌，辅助热气流及惰性气体低温贮藏。Zhang
Figure 615c_1
, Wang Yuyu, Sun Lei, Wang Xianglian, Liu Chunquan (application number: 201710098334.0) discloses a method for prolonging the shelf life of salted geese by pasteurization combined with nano zinc oxide bacteriostatic and radio frequency sterilization, and the goose meat of the invention is subjected to low temperature pasteurization. Before vacuum packaging, add nano-zinc oxide to inhibit bacteria, vacuum-package and then radio-frequency sterilization. The method avoids the problem of high quality destruction of goose meat by using high temperature and high pressure sterilization, and can prolong the shelf life of 25 ° C of salt water goose to 5-6 months, and the shelf life of 4 ° C to 11-12 months, while maximizing The quality of the goose is guaranteed, and the preservation rate of flavor, color, taste and texture is about 93%. The present invention differs from the invention in that ozone is synergistically sterilized, and the hot gas stream and the inert gas are stored at a low temperature.
以上主要是利用联合协同柔性杀菌技术处理麦苗粉，而本专利通过臭氧协同杀菌和惰性气体保护等方法，保护了麦苗粉的品质及超氧崎化酶（SOD）的活性。The above mainly uses the combined synergistic flexible sterilization technology to treat the wheat seedling powder, and the patent protects the quality of the wheat seedling powder and the activity of superoxide-salting enzyme (SOD) by means of ozone synergistic sterilization and inert gas protection.
技术问题technical problem
本发明的目的是降低麦苗粉杀菌的强度，提高其品质营养价值，保持SOD的活性，利用臭氧协同射频杀菌技术，添加SOD活性保护剂杨梅多酚，采用组方惰性气体保护，避光低温贮藏保持超氧化物歧化酶活性的速溶调理麦绿粉产品。The purpose of the invention is to reduce the sterilization intensity of the wheat seed powder, improve the nutritional value of the quality, maintain the activity of the SOD, use the ozone synergistic radio frequency sterilization technology, add the SOD active protective agent bayberry polyphenol, protect the group by inert gas, avoid the light and store at low temperature. Instantly conditioned green wheat flour product that maintains superoxide dismutase activity.
技术解决方案Technical solution
本发明的技术方案：The technical solution of the invention:
一种喷雾干燥调理麦苗粉柔性杀菌及营养组分有效保存的方法，其特征在于，该方法包括除杂、组方、装载、通入臭氧及变速搅拌、两波段射频杀菌、自然冷却、分装、惰性气体保护、密封和避光低温贮藏，具体步骤如下：The invention relates to a method for flexible sterilization of spray-dried conditioning wheat seedling powder and effective preservation of nutrient components, characterized in that the method comprises impurity removal, assembly, loading, ozone and variable speed stirring, two-band radio frequency sterilization, natural cooling, and dispensing , inert gas protection, sealing and protection from light and low temperature storage, the specific steps are as follows:
（1）除杂：将幼年大麦茎叶汁进行喷雾干燥处理得到麦苗粉，将10-20µm的麦苗粉除去金属杂质；(1) Removal: the young barley stem and leaf juice is spray-dried to obtain the wheat seed powder, and the 10-20 μm wheat seed powder is removed from the metal impurities;
具体过程如下：将麦苗粉平铺在振筛的进料托盘上；麦苗粉通过直径为3cm的磁力吸附通道进入筛网，筛网在振动频率为30Hz下，震动1-2min，吸附除去麦苗粉中的金属杂质，随后收集；振筛呈直径2m的圆形，振筛上钢网的开孔率为0.8，孔径为1mm；The specific process is as follows: the wheat seedling powder is spread on the feeding tray of the vibrating screen; the wheat seedling powder enters the sieve through a magnetic adsorption channel having a diameter of 3 cm, and the sieve mesh is shaken at a frequency of 30 Hz for 1-2 min to adsorb and remove the wheat seed powder. The metal impurities in the subsequent collection; the vibrating screen is a circle having a diameter of 2 m, and the opening ratio of the steel mesh on the vibrating screen is 0.8, and the pore diameter is 1 mm;
（2）组方：向步骤（1）收集的麦苗粉中加入抗氧化物质杨梅多酚及营养物质，抗氧化物质杨梅多酚的含量为0.5-1.5%；加入后用搅拌机混合均匀，先在转速100rpm下搅拌1-2min，然后在300rpm下搅拌10-20s；其中，搅拌机功率为700W，转速为10-500rpm；(2) Formula: Add the antioxidant substance, bayberry polyphenols and nutrients to the wheat seed powder collected in step (1). The content of the antioxidant substance, bayberry polyphenol, is 0.5-1.5%; after mixing, mix with a blender, first in the Stirring at rpm for 1-2 min, then stirring at 300 rpm for 10-20 s; wherein, the mixer power is 700 W, the rotational speed is 10-500 rpm;
（3）装载：将步骤（2）所得麦苗粉装入容器中，容器口塞上双孔聚氟乙烯塞子；其中，容器直径为30cm，高度为8cm；(3) loading: the wheat seedling powder obtained in the step (2) is placed in a container, the container mouth is plugged with a two-hole polyvinyl fluoride stopper; wherein the container has a diameter of 30 cm and a height of 8 cm;
（4）通入臭氧及变速搅拌：连接好臭氧发生器和塞子，向步骤（3）容器中通入臭氧并同时搅拌，在充臭氧条件下，先以10-30rpm的搅拌速度搅拌20-60s；然后以30-50rpm的搅拌速度搅拌20-60s；其中，臭氧浓度为10-20ppm，充气速度为1-3ml/s；(4) Passing ozone and variable speed stirring: connect the ozone generator and the plug, and put ozone into the vessel of step (3) and stir at the same time. Under the condition of ozone charging, stir the stirring speed at 10-30 rpm for 20-60 s. And then stirred at a stirring speed of 30-50 rpm for 20-60 s; wherein, the ozone concentration is 10-20 ppm, the aeration rate is 1-3 ml / s;
（5）两波段射频杀菌：对步骤（4）臭氧处理的麦苗粉进行两波段射频杀菌处理，先在27.12MHz下杀菌3-10min，然后在40.68MHz下杀菌2-15min；其中，射频极板间距为12-14cm，辅助气流热温度为45-55℃；(5) Two-band radio frequency sterilization: the two-band radio frequency sterilization treatment of the step (4) ozone-treated wheat seed powder is first sterilized at 27.12 MHz for 3-10 min, and then sterilized at 40.68 MHz for 2-15 min; wherein, the radio frequency plate The spacing is 12-14cm, and the auxiliary airflow heat temperature is 45-55 ° C;
（6）自然冷却：将步骤（5）杀菌过的麦苗粉自然冷却至室温；(6) Natural cooling: the wheat seed powder sterilized in the step (5) is naturally cooled to room temperature;
（7）分装：在无菌条件下，将冷却后的麦苗粉称量后分装于锡箔袋中；(7) Packing: Under sterile conditions, the cooled wheat seedling powder is weighed and then dispensed into a tin foil bag;
（8）惰性气体保护：从锡箔袋底部缓慢充入复合惰性气体，用复合惰性气体置换出袋中氧气；(8) Inert gas protection: slowly fill the composite inert gas from the bottom of the foil bag, and replace the oxygen in the bag with the composite inert gas;
（9）密封：经复合惰性气体置换后，立即热塑封装密封；(9) Sealing: Immediately after being replaced by a composite inert gas, the thermoplastic package is sealed;
（10）避光低温贮藏：将步骤（7）所得麦苗粉，在0-15℃下避光贮藏。(10) Low-temperature storage in the dark: The wheat seed powder obtained in the step (7) is stored at 0-15 ° C in the dark.
步骤（4）和步骤（5）组合处理的方式分为三种情况，具体为：The combination processing of step (4) and step (5) is divided into three cases, specifically:
大距离臭氧协助射频杀菌：步骤（5）中，当射频极板间距为14cm时，先在27.12MHz下杀菌5min，然后在48.68MHz下杀菌10min，辅助气流热温度为55℃；步骤（4）中，麦苗粉在充臭氧条件下，先以10-30rpm的搅拌速度搅拌20-60s，然后以20-50rpm的搅拌速度搅拌20-60s，臭氧浓度为20ppm，充气流速度为1-3ml/s；Large distance ozone assists radio frequency sterilization: In step (5), when the RF plate spacing is 14cm, first sterilize at 27.12MHz for 5min, then sterilize at 48.68MHz for 10min, and the auxiliary airflow heat temperature is 55 °C; step (4) In the ozone-filling condition, the wheat seedling powder is first stirred at a stirring speed of 10-30 rpm for 20-60 s, then stirred at a stirring speed of 20-50 rpm for 20-60 s, the ozone concentration is 20 ppm, and the aeration flow rate is 1-3 ml/s. ;
中距离臭氧协助射频杀菌：步骤（5）中，当射频极板间距为13cm时，先在27.12MHz下杀菌5min，然后在48.68MHz下杀菌8min，辅助气流热温度为55℃；步骤（4）中，麦苗粉在充臭氧条件下，先以10-30rpm的搅拌速度搅拌20-60s，然后以20-50rpm的搅拌速度搅拌20-60s，臭氧浓度为15ppm，充气速度为1-3ml/s；Medium distance ozone assisted radio frequency sterilization: In step (5), when the RF plate spacing is 13cm, first sterilize at 27.12MHz for 5min, then sterilize at 48.68MHz for 8min, and the auxiliary airflow heat temperature is 55 °C; step (4) The wheat seedling powder is stirred under the condition of ozone charging for 20-60 s at a stirring speed of 10-30 rpm, then stirred at a stirring speed of 20-50 rpm for 20-60 s, the ozone concentration is 15 ppm, and the aeration speed is 1-3 ml/s;
小距离臭氧协助射频杀菌：步骤（5）中，当射频极板间距为12cm时，先在27.12MHz下杀菌5min，然后在48.68Mhz下杀菌5min，辅助热气流热温度为55℃；步骤（4）中，，麦苗粉在充臭氧条件下，先以10-30rpm的搅拌速度搅拌20-60s，然后以20-50rpm的搅拌速度搅拌20-60s，臭氧浓度为10ppm，气流速度为1-3ml/s；Small distance ozone assists radio frequency sterilization: In step (5), when the RF plate spacing is 12cm, first sterilize at 27.12MHz for 5min, then sterilize at 48.68Mhz for 5min, and the auxiliary hot gas heat temperature is 55°C; step (4) In the ozone-filling condition, the wheat seedling powder is first stirred at a stirring speed of 10-30 rpm for 20-60 s, then stirred at a stirring speed of 20-50 rpm for 20-60 s, the ozone concentration is 10 ppm, and the gas flow rate is 1-3 ml/ s;
制得的麦苗粉中叶绿素含量、总黄酮含量和SOD活力具体为：采用大距离臭氧协助射频杀菌时制得的麦苗粉中：叶绿素含量范围500~550mg/100g，总黄酮含量范围450~480mg/100g， SOD活力300-380U/mg，维生素C 0.5%，与杀菌前相比，保存率均高于93%。The chlorophyll content, total flavonoid content and SOD activity of the obtained wheat seedling powder are specifically as follows: the wheat seed powder obtained by using large distance ozone to assist radiofrequency sterilization: the chlorophyll content ranges from 500 to 550 mg/100 g, and the total flavonoid content ranges from 450 to 480 mg/ 100g, SOD activity 300-380U/mg, vitamin C 0.5%, the preservation rate was higher than 93% compared with before sterilization.
采用中距离臭氧协助射频杀菌时制得的麦苗粉中：叶绿素含量范围500~540mg/100g，总黄酮含量范围470~510mg/100g，SOD活力290-380U/mg，维生素C 1%，与杀菌前相比，保存率均高于90%。In the wheat seedling powder prepared by medium-distance ozone assisted radiofrequency sterilization: chlorophyll content range 500~540mg/100g, total flavonoid content range 470~510mg/100g, SOD activity 290-380U/mg, vitamin C 1%, and before sterilization In comparison, the preservation rate is higher than 90%.
采用小距离臭氧协助射频杀菌时制得的麦苗粉中：叶绿素含量范围500~530mg/100g，黄酮含量范围450~510mg/100g，SOD活力250-380U/mg，维生素C 2%，与杀菌前相比，保存率均高于90%。The wheat seedling powder prepared by using small distance ozone to assist radiofrequency sterilization: chlorophyll content range 500~530mg/100g, flavonoid content range 450~510mg/100g, SOD activity 250-380U/mg, vitamin C 2%, and pre-sterilization phase The retention rate is higher than 90%.
以体积比计，步骤（7）的复合惰性气体配方为：The composite inert gas formulation of step (7) is: by volume ratio:
高氮气惰性气体配方：氮气:氩气=9:1，氮气、氩气纯度均≥99.5%。High nitrogen inert gas formula: nitrogen: argon = 9:1, nitrogen, argon purity are ≥ 99.5%.
中氮气惰性气体配方：氮气:氩气=8:2，氮气、氩气纯度均≥99.5%。Medium nitrogen inert gas formula: nitrogen: argon = 8:2, nitrogen, argon purity are ≥ 99.5%.
低氮气惰性气体配方： 氮气:氩气=6:4，氮气、氩气纯度均≥99.5%。Low nitrogen inert gas formula: Nitrogen: Argon = 6:4, nitrogen, argon purity are ≥ 99.5%.
有益效果Beneficial effect
本发明的有益效果：The beneficial effects of the invention:
（1）与传统提取方法相比，采用喷雾干燥制造麦苗粉，干燥时间短，营养素高、功能成分损失小。(1) Compared with the traditional extraction method, the wheat seedling powder is spray-dried, the drying time is short, the nutrient is high, and the functional component loss is small.
（2）与传统的热杀菌相比，采用了射频杀菌和抑菌纳米锌物质方法，其对营养成分和SOD的活力破坏降低，保持好的品质。(2) Compared with traditional heat sterilization, the method of radiofrequency sterilization and bacteriostatic nano-zinc material is adopted, which reduces the damage of nutrients and SOD vitality and maintains good quality.
（3）根据不同人群的需求，添加一些辅料，不仅提高了麦苗粉的品质，还使产品更加具有针对性，提高产品的附加值。(3) According to the needs of different groups of people, adding some excipients not only improves the quality of the wheat seedling powder, but also makes the products more targeted and increases the added value of the products.
（3）添加了抗氧化物质杨梅多酚，保持了SOD活力，提高了产品的功能效果，更能提高人体的免疫力。(3) The antioxidant substance Yangmei polyphenol is added, which maintains the SOD activity, improves the functional effect of the product, and improves the immunity of the human body.
本发明的实施方式Embodiments of the invention
以下结合具体实施方式对本发明做进一步说明。The invention will be further described below in conjunction with specific embodiments.
实施例1大距离臭氧协助射频杀菌及高氮气惰性气体保护的杀菌保藏方法Example 1 Sterilization and preservation method for large distance ozone assisted radio frequency sterilization and high nitrogen inert gas protection
取1kg喷雾干燥麦苗粉，平铺磁选振筛机上，在30Hz震动频率下，震动2min，收集麦苗粉。添加抗氧化物质杨梅多酚的含量为0.5%；组方后用搅拌机混合均匀，100rpm搅拌时间1.5min，300rpm搅拌20s。在25℃室温下，装入杀菌玻璃容器，塞上聚氟乙烯塞子。用皮管连接好臭氧发生器和杀菌塞子，皮管直径0.5cm，长度1m；搅拌且同时通入臭氧；臭氧浓度20ppm， 进气速度3ml/s，搅拌速度20rpm，搅拌时间30s，然后搅拌速度40rpm，搅拌时间30s。搅拌后用塞子密封玻璃容器，设置辅助热气流温度55℃， 在27.12MHz射频杀菌7min，然后在40.78MHz射频杀菌8min；射频极板间距14cm，功率6kW；干燥腔中自然冷却后，分装于锡箔袋子中；从锡箔密封袋的底部缓慢配方惰性气体，并迅速热塑密封；气体气流量1ml/s，时间10s；气体组方：氮气：氩气=90：10（体积比），氮气、氩气纯度均≥99.5%。杀菌后，每袋100g的产品避光低温贮藏，保质期12月以上。Take 1kg of spray-dried wheat seedling powder, spread it on a magnetically selected vibrating screen machine, shake it at a vibration frequency of 30Hz for 2min, and collect the wheat seedling powder. The content of the antioxidant substance, bayberry polyphenol, was 0.5%; the mixture was uniformly mixed with a mixer, stirred at 100 rpm for 1.5 min, and stirred at 300 rpm for 20 s. The mixture was placed in a sterilizing glass container at room temperature of 25 ° C, and a polyvinyl fluoride stopper was placed. Connect the ozone generator and the sterilization plug with a leather tube. The diameter of the skin tube is 0.5cm and the length is 1m. Stirring and simultaneously introducing ozone; ozone concentration 20ppm, inlet speed 3ml/s, stirring speed 20rpm, stirring time 30s, then stirring speed 40 rpm, stirring time 30 s. After stirring, seal the glass container with a stopper, set the auxiliary hot air temperature to 55 ° C, radio sterilize at 27.12 MHz for 7 min, then radio sterilize at 40.78 MHz for 8 min; the RF plate spacing is 14 cm, the power is 6 kW; after cooling in the drying chamber, it is divided into In the foil bag; slowly inject inert gas from the bottom of the foil sealed bag and quickly thermoplastic seal; gas flow rate 1ml / s, time 10s; gas group: nitrogen: argon = 90: 10 (volume ratio), nitrogen, The purity of argon is ≥99.5%. After sterilization, 100g of each bag is stored in the dark and protected from light for a period of more than 12 months.
叶绿素含量范围500~550mg/100g，总黄酮含量范围450~480mg/100g， SOD活力370U/mg，维生素C 0.5%，与杀菌前相比，叶绿素、黄酮和超氧崎化酶的抗氧化活性保存率均高于93%。此产品颜色翠绿，有浓烈的麦清香，颗粒均匀，在30℃水中，20s内快速溶解，无任何沉淀，麦汁汤有光泽。The chlorophyll content ranged from 500 to 550 mg/100 g, the total flavonoid content ranged from 450 to 480 mg/100 g, the SOD activity was 370 U/mg, and the vitamin C was 0.5%. The antioxidant activity of chlorophyll, flavonoids and superoxide-enriched enzyme was preserved compared with that before sterilization. The rate is higher than 93%. This product is green in color, has strong wheat scent, uniform particles, dissolves rapidly in water at 30 ° C for 20 s without any precipitation, and the wort soup is shiny.
实施例2中距离臭氧协助射频杀菌及高氮气惰性气体保护的杀菌保藏方法Sterilization and preservation method for ozone sterilization assisted radio frequency sterilization and high nitrogen inert gas protection in Example 2
取2kg喷雾干燥麦苗粉，平铺磁选振筛机上，在30Hz震动频率下，震动2min，收集麦苗粉。添加抗氧化物质杨梅多酚的含量为1.0%；组方后用搅拌机混合均匀，100rpm搅拌时间2min，300rpm搅拌15s。在25℃室温下，装入杀菌玻璃容器，塞上聚氟乙烯塞子。用皮管连接好臭氧发生器和杀菌塞子，皮管直径0.5cm，长度1m；搅拌且同时通入臭氧；臭氧浓度15ppm， 进气速度3ml/s，搅拌速度10rpm，搅拌时间30s，50rpm搅拌30s。然后拔掉搅拌辅助及通气管用胶塞密封样品于玻璃容器中。然后设置辅助热气流温度50℃，在27.12MHz射频杀菌5min，然后在40.78MHz射频杀菌5min；射频极板间距13cm，功率6kW；干燥腔中自然冷却后，分装于锡箔袋中；从锡箔密封袋的底部缓慢配方惰性气体，并迅速热塑密封；气体气流量1ml/s，时间10s；气体组方：氮气：氩气=90：10（体积比），氮气、氩气纯度均≥99.5%。杀菌后，每袋100g的产品避光低温贮藏，保质期12月以上。Take 2kg of spray-dried wheat seedling powder, spread on a magnetically selected vibrating screen machine, shake at 2Hz vibration frequency for 2min, and collect the wheat seedling powder. The content of the antioxidant-resistant ramisin was 1.0%; the mixture was uniformly mixed with a mixer, stirred at 100 rpm for 2 min, and stirred at 300 rpm for 15 s. The mixture was placed in a sterilizing glass container at room temperature of 25 ° C, and a polyvinyl fluoride stopper was placed. Connect the ozone generator and the sterilization plug with a leather tube. The diameter of the skin tube is 0.5cm and the length is 1m. Stirring and simultaneously introducing ozone; the ozone concentration is 15ppm, the inlet speed is 3ml/s, the stirring speed is 10rpm, the stirring time is 30s, and the stirring is 30s. . Then unplug the mixing aid and the vent tube to seal the sample in a glass container with a rubber stopper. Then set the auxiliary hot air temperature to 50 ° C, radio frequency sterilization at 27.12 MHz for 5 min, then radio frequency sterilization for 5 min at 40.78 MHz; radio frequency plate spacing 13 cm, power 6 kW; natural cooling in the drying chamber, sub-packaged in tin foil bags; sealed from tin foil The bottom of the bag is slowly formulated with inert gas and rapidly thermoplastically sealed; the gas flow rate is 1ml/s for 10s; the gas group is: nitrogen: argon = 90:10 (volume ratio), and the purity of nitrogen and argon is ≥99.5%. . After sterilization, 100g of each bag is stored in the dark and protected from light for a period of more than 12 months.
叶绿素含量范围500~540mg/100g，总黄酮含量范围470~510mg/100g，SOD活力350U/mg，维生素C 1%。与杀菌前相比，叶绿素、黄酮和超氧崎化酶的抗氧化活性保存率均高于90%；产品可快速（10s）溶解分散在50℃的温水中，无结块，无明显沉底现象。The chlorophyll content ranged from 500 to 540 mg/100 g, the total flavonoid content ranged from 470 to 510 mg/100 g, the SOD activity was 350 U/mg, and the vitamin C was 1%. Compared with pre-sterilization, the antioxidant activity retention rates of chlorophyll, flavonoids and superoxide-enriched enzymes were all higher than 90%; the product could be dissolved quickly (10s) in warm water at 50 °C without agglomeration, no obvious sinking phenomenon.
实施例3：小距离臭氧协助射频杀菌及高氮气惰性气体保护的杀菌保藏方法Example 3: Sterilization and preservation method for small distance ozone assisted radio frequency sterilization and high nitrogen inert gas protection
取1.5千克喷雾干燥麦苗粉，平铺于磁选振筛机上，在30Hz震动频率下，振筛2min，收集麦苗粉。添加抗氧化物质杨梅多酚的含量为1.5%；组方后用搅拌机混合均匀；100rpm搅拌时间1.5min，300rpm搅拌20s。在25℃室温下，装入杀菌玻璃容器，塞上聚氟乙烯塞子。用皮管连接好臭氧发生器和杀菌塞子，皮管直径0.5cm，长度1m；搅拌同时通入臭氧；臭氧浓度10ppm，进气速度3ml/s，搅拌速度20rpm，搅拌时间30s，然后搅拌速度40rpm，搅拌30s。变速搅拌后封口射频杀菌，设置辅助热气流温度55℃，在27.12MHz射频杀菌4min，然后在40.78MHz射频杀菌4min；射频极板间距12cm，功率6kW；自然冷却后，分装于锡箔袋中；从锡箔密封袋的底部缓慢配方惰性气体，并迅速热塑密封；气体气流量1ml/s，时间10s；气体组方：氮气：氩气=90：10（体积比），氮气、氩气纯度均≥99.5%。杀菌后，每袋100g的产品避光低温贮藏，保质期12月以上。1.5 kg of spray-dried wheat seedling powder was taken and spread on a magnetic vibrating sieve machine, and shaken at a vibration frequency of 30 Hz for 2 min to collect the wheat seed powder. The content of the anti-oxidant arbutin was 1.5%; the mixture was uniformly mixed with a mixer; the stirring time was 1.5 rpm at 100 rpm, and the mixture was stirred at 300 rpm for 20 s. The mixture was placed in a sterilizing glass container at room temperature of 25 ° C, and a polyvinyl fluoride stopper was placed. Connect the ozone generator and the sterilization plug with a leather tube. The diameter of the skin tube is 0.5cm and the length is 1m. The ozone is added to the mixture while stirring; the ozone concentration is 10ppm, the inlet speed is 3ml/s, the stirring speed is 20rpm, the stirring time is 30s, then the stirring speed is 40rpm. Stir for 30 s. After variable speed stirring, the sealing RF sterilization, set the auxiliary hot air temperature to 55 ° C, radio frequency sterilization at 27.12 MHz for 4 min, then radio sterilization at 40.78 MHz for 4 min; RF plate spacing 12 cm, power 6 kW; The inert gas is slowly formulated from the bottom of the tin foil sealed bag, and is rapidly thermoplastically sealed; the gas flow rate is 1 ml/s for 10 s; the gas group side: nitrogen: argon = 90:10 (volume ratio), and the purity of nitrogen and argon are both ≥99.5%. After sterilization, 100g of each bag is stored in the dark and protected from light for a period of more than 12 months.
叶绿素含量范围500~530mg/100g，黄酮含量范围490~510mg/100g，SOD活力330U/mg，维生素C 2%，与杀菌前相比，叶绿素、黄酮和超氧崎化酶的抗氧化活性保存率均高于90%；产品颜色翠绿，麦苗香气浓烈，口感润滑清凉，在30℃温度下，10s能全部溶解，无沉淀。The chlorophyll content ranged from 500 to 530 mg/100 g, the flavonoid content ranged from 490 to 510 mg/100 g, the SOD activity was 330 U/mg, and the vitamin C was 2%. The antioxidant activity retention rate of chlorophyll, flavonoids and superoxide-enriched enzyme was compared with that before sterilization. Both are higher than 90%; the product color is green, the wheat aroma is strong, the taste is lubricated and cool, at 30 ° C, 10s can be completely dissolved, no precipitation.

Claims (6)

  1. 一种喷雾干燥调理麦苗粉柔性杀菌及营养组分有效保存的方法，其特征在于，具体步骤如下：The invention relates to a method for flexible sterilization of spray-dried conditioning wheat seed powder and effective preservation of nutrient components, characterized in that the specific steps are as follows:
    （1）除杂：将幼年大麦茎叶汁进行喷雾干燥处理得到麦苗粉，将10-20µm的麦苗粉除去金属杂质；(1) Removal: the young barley stem and leaf juice is spray-dried to obtain the wheat seed powder, and the 10-20 μm wheat seed powder is removed from the metal impurities;
    （2）组方：向步骤（1）收集的麦苗粉中加入抗氧化物质杨梅多酚及营养物质，抗氧化物质杨梅多酚的含量为0.5-1.5%；加入后用搅拌机混合均匀，先在转速100rpm下搅拌1-2min，然后在300rpm下搅拌10-20s；其中，搅拌机功率为700W，转速为10-500rpm；(2) Formula: Add the antioxidant substance, bayberry polyphenols and nutrients to the wheat seed powder collected in step (1). The content of the antioxidant substance, bayberry polyphenol, is 0.5-1.5%; after mixing, mix with a blender, first in the Stirring at rpm for 1-2 min, then stirring at 300 rpm for 10-20 s; wherein, the mixer power is 700 W, the rotational speed is 10-500 rpm;
    （3）装载：将步骤（2）所得麦苗粉装入容器中，容器口塞上双孔聚氟乙烯塞子；其中，容器直径为30cm，高度为8cm；(3) loading: the wheat seedling powder obtained in the step (2) is placed in a container, the container mouth is plugged with a two-hole polyvinyl fluoride stopper; wherein the container has a diameter of 30 cm and a height of 8 cm;
    （4）通入臭氧及变速搅拌：连接好臭氧发生器和塞子，向步骤（3）容器中通入臭氧并同时搅拌，在充臭氧条件下，先以10-30rpm的搅拌速度搅拌20-60s；然后以30-50rpm的搅拌速度搅拌20-60s；其中，臭氧浓度为10-20ppm，充气速度为1-3ml/s；(4) Passing ozone and variable speed stirring: connect the ozone generator and the plug, and put ozone into the vessel of step (3) and stir at the same time. Under the condition of ozone charging, stir the stirring speed at 10-30 rpm for 20-60 s. And then stirred at a stirring speed of 30-50 rpm for 20-60 s; wherein, the ozone concentration is 10-20 ppm, the aeration rate is 1-3 ml / s;
    （5）两波段射频杀菌：对步骤（4）臭氧处理的麦苗粉进行两波段射频杀菌处理，先在27.12MHz下杀菌3-10min，然后在40.68MHz下杀菌2-15min；其中，射频极板间距为12-14cm，辅助气流热温度为45-55℃；(5) Two-band radio frequency sterilization: the two-band radio frequency sterilization treatment of the step (4) ozone-treated wheat seed powder is first sterilized at 27.12 MHz for 3-10 min, and then sterilized at 40.68 MHz for 2-15 min; wherein, the radio frequency plate The spacing is 12-14cm, and the auxiliary airflow heat temperature is 45-55 ° C;
    （6）自然冷却：将步骤（5）杀菌过的麦苗粉自然冷却至室温；(6) Natural cooling: the wheat seed powder sterilized in the step (5) is naturally cooled to room temperature;
    （7）分装：在无菌条件下，将冷却后的麦苗粉称量后分装于锡箔袋中；(7) Packing: Under sterile conditions, the cooled wheat seedling powder is weighed and then dispensed into a tin foil bag;
    （8）惰性气体保护：从锡箔袋底部缓慢充入复合惰性气体，用复合惰性气体置换出袋中氧气；(8) Inert gas protection: slowly fill the composite inert gas from the bottom of the foil bag, and replace the oxygen in the bag with the composite inert gas;
    （9）密封：经复合惰性气体置换后，立即热塑封装密封； (9) Sealing: Immediately after being replaced by a composite inert gas, the thermoplastic package is sealed;
    （10）避光低温贮藏：将步骤（7）所得麦苗粉，在0-15℃下避光贮藏。(10) Low-temperature storage in the dark: The wheat seed powder obtained in the step (7) is stored at 0-15 ° C in the dark.
  2. 根据权利要求1所述的一种喷雾干燥调理麦苗粉柔性杀菌及营养组分有效保存的方法，其特征在于，步骤（4）和步骤（5）组合处理的方式分为三种情况，具体为：The method for the flexible sterilization of the spray-dried conditioning wheat seedling powder and the effective preservation of the nutrient component according to claim 1, wherein the combination of the steps (4) and (5) is divided into three cases, specifically :
    大距离臭氧协助射频杀菌：步骤（5）中，当射频极板间距为14cm时，先在27.12MHz下杀菌5min，然后在48.68MHz下杀菌10min，辅助气流热温度为55℃；步骤（4）中，麦苗粉在充臭氧条件下，先以10-30rpm的搅拌速度搅拌20-60s，然后以20-50rpm的搅拌速度搅拌20-60s，臭氧浓度为20ppm，充气速度为1-3ml/s；Large distance ozone assists radio frequency sterilization: In step (5), when the RF plate spacing is 14cm, first sterilize at 27.12MHz for 5min, then sterilize at 48.68MHz for 10min, and the auxiliary airflow heat temperature is 55 °C; step (4) Wherein, the wheat seedling powder is stirred under stirring conditions at a stirring speed of 10-30 rpm for 20-60 s, then stirred at a stirring speed of 20-50 rpm for 20-60 s, an ozone concentration of 20 ppm, and an aeration rate of 1-3 ml/s;
    中距离臭氧协助射频杀菌：步骤（5）中，当射频极板间距为13cm时，先在27.12MHz下杀菌5min，然后在48.68MHz下杀菌8min，辅助气流热温度为55℃；步骤（4）中，麦苗粉在充臭氧条件下，先以10-30rpm的搅拌速度搅拌20-60s，然后以20-50rpm的搅拌速度搅拌20-60s，臭氧浓度为15ppm，充气速度为1-3ml/s；Medium distance ozone assisted radio frequency sterilization: In step (5), when the RF plate spacing is 13cm, first sterilize at 27.12MHz for 5min, then sterilize at 48.68MHz for 8min, and the auxiliary airflow heat temperature is 55 °C; step (4) The wheat seedling powder is stirred under the condition of ozone charging for 20-60 s at a stirring speed of 10-30 rpm, then stirred at a stirring speed of 20-50 rpm for 20-60 s, the ozone concentration is 15 ppm, and the aeration speed is 1-3 ml/s;
    小距离臭氧协助射频杀菌：步骤（5）中，当射频极板间距为12cm时，先在27.12MHz下杀菌5min，然后在48.68MHz下杀菌5min，辅助热气流热温度为55℃；步骤（4）中，麦苗粉在充臭氧条件下，先以10-30rpm的搅拌速度搅拌20-60s，然后以20-50rpm的搅拌速度搅拌20-60s，臭氧浓度为10ppm气流速度为1-3ml/s。Small distance ozone assists radio frequency sterilization: in step (5), when the RF plate spacing is 12cm, first sterilize at 27.12MHz for 5min, then sterilize at 48.68MHz for 5min, and the auxiliary hot gas heat temperature is 55°C; step (4) In the ozone charging condition, the wheat seed powder is first stirred at a stirring speed of 10-30 rpm for 20-60 s, then stirred at a stirring speed of 20-50 rpm for 20-60 s, and the ozone concentration is 10 ppm at a gas flow rate of 1-3 ml/s.
  3. 根据权利要求2所述的方法，其特征在于，制得的麦苗粉中叶绿素含量、总黄酮含量和SOD活力具体为：The method according to claim 2, wherein the chlorophyll content, the total flavonoid content and the SOD activity of the obtained wheat seed powder are specifically:
    采用大距离臭氧协助射频杀菌时制得的麦苗粉中：叶绿素含量范围500~550mg/100g，总黄酮含量范围450~480mg/100g，SOD活力300-380U/mg，维生素C 0.5%，与杀菌前相比，保存率均高于93%；In the wheat seedling powder prepared by large distance ozone to assist radiofrequency sterilization: chlorophyll content range is 500~550mg/100g, total flavonoid content range is 450~480mg/100g, SOD activity is 300-380U/mg, vitamin C is 0.5%, and before sterilization In comparison, the preservation rate is higher than 93%;
    采用中距离臭氧协助射频杀菌时制得的麦苗粉中：叶绿素含量范围500~540mg/100g，总黄酮含量范围470~510mg/100g，SOD活力290-380U/mg，维生素C 1%与杀菌前相比，保存率均高于90%；In the wheat seedling powder prepared by medium-distance ozone assisted radiofrequency sterilization: chlorophyll content range 500~540mg/100g, total flavonoid content range 470~510mg/100g, SOD activity 290-380U/mg, vitamin C 1% and pre-sterilization phase Ratio, the preservation rate is higher than 90%;
    采用小距离臭氧协助射频杀菌时制得的麦苗粉中：叶绿素含量范围500~530mg/100g，黄酮含量范围450~510mg/100g，SOD活力250-380U/mg，维生素C 2%，与杀菌前相比，保存率均高于90%。The wheat seedling powder prepared by using small distance ozone to assist radiofrequency sterilization: chlorophyll content range 500~530mg/100g, flavonoid content range 450~510mg/100g, SOD activity 250-380U/mg, vitamin C 2%, and pre-sterilization phase The retention rate is higher than 90%.
  4. 根据权利要求1-3任一所述的方法，其特征在于，以体积比计，步骤（7）的复合惰性气体配方为：The method according to any one of claims 1 to 3, characterized in that the composite inert gas formulation of the step (7) is: by volume ratio:
    高氮气惰性气体配方：氮气:氩气=9:1，氮气、氩气纯度均≥99.5%；High nitrogen inert gas formula: nitrogen: argon = 9:1, nitrogen, argon purity are ≥ 99.5%;
    中氮气惰性气体配方：氮气:氩气=8:2，氮气、氩气纯度均≥99.5%；Medium nitrogen inert gas formula: nitrogen: argon = 8:2, nitrogen, argon purity are ≥ 99.5%;
    低氮气惰性气体配方：氮气:氩气=6:4，氮气、氩气纯度均≥99.5%。Low nitrogen inert gas formula: nitrogen: argon = 6:4, nitrogen, argon purity are ≥ 99.5%.
  5. 根据权利要求1-3任一所述的方法，其特征在于，所述的除杂具体过程如下：The method according to any one of claims 1-3, wherein the specific process of removing the impurities is as follows:
    将10-20µm的麦苗粉平铺在振筛的进料托盘上；麦苗粉通过直径为3cm的磁力吸附通道进入筛网，筛网在振动频率为30Hz下，震动1-2min，吸附除去麦苗粉中的金属杂质，随后收集；振筛呈直径2m的圆形，振筛上钢网的开孔率为0.8，孔径为1mm。The 10-20 μm wheat seed powder was spread on the feeding tray of the vibrating screen; the wheat seed powder entered the screen through a magnetic adsorption channel with a diameter of 3 cm, and the sieve was shaken at a vibration frequency of 30 Hz for 1-2 min to remove the wheat seed powder. The metal impurities in the collection were subsequently collected; the vibrating screen was circular with a diameter of 2 m, and the opening ratio of the steel mesh on the vibrating screen was 0.8, and the pore diameter was 1 mm.
  6. 根据权利要求4所述的方法，其特征在于，所述的除杂具体过程如下：将10-20µm的麦苗粉平铺在振筛的进料托盘上；麦苗粉通过直径为3cm的磁力吸附通道进入筛网，筛网在振动频率为30Hz下，震动1-2min，吸附除去麦苗粉中的金属杂质，随后收集；振筛呈直径2m的圆形，振筛上钢网的开孔率为0.8，孔径为1mm。The method according to claim 4, wherein the removing process is as follows: 10-20 μm of the wheat seed powder is spread on the feeding tray of the vibrating screen; the wheat seed powder passes through a magnetic adsorption channel having a diameter of 3 cm. After entering the screen, the screen vibrates at a vibration frequency of 30 Hz, shaking for 1-2 min, adsorbing and removing the metal impurities in the wheat seed powder, and then collecting; the vibrating screen is circular with a diameter of 2 m, and the opening ratio of the steel mesh on the vibrating screen is 0.8. The aperture is 1mm.
PCT/CN2018/093125 2018-03-05 2018-06-27 Method for flexible sterilization of spray-dried-and-prepared barley grass powder and effective storage of nutrition component WO2019169807A1 (en)

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