WO2019157828A1 - 生物片材固定装置 - Google Patents

生物片材固定装置 Download PDF

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Publication number
WO2019157828A1
WO2019157828A1 PCT/CN2018/110563 CN2018110563W WO2019157828A1 WO 2019157828 A1 WO2019157828 A1 WO 2019157828A1 CN 2018110563 W CN2018110563 W CN 2018110563W WO 2019157828 A1 WO2019157828 A1 WO 2019157828A1
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WO
WIPO (PCT)
Prior art keywords
fixing device
biological
biological sheet
sheet
sheet fixing
Prior art date
Application number
PCT/CN2018/110563
Other languages
English (en)
French (fr)
Inventor
周适
Original Assignee
京东方科技集团股份有限公司
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 京东方科技集团股份有限公司 filed Critical 京东方科技集团股份有限公司
Priority to US16/757,405 priority Critical patent/US20210008563A1/en
Publication of WO2019157828A1 publication Critical patent/WO2019157828A1/zh

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Classifications

    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N1/00—Preservation of bodies of humans or animals, or parts thereof
    • A01N1/02—Preservation of living parts
    • A01N1/0236—Mechanical aspects
    • A01N1/0242—Apparatuses, i.e. devices used in the process of preservation of living parts, such as pumps, refrigeration devices or any other devices featuring moving parts and/or temperature controlling components
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N1/00—Preservation of bodies of humans or animals, or parts thereof
    • A01N1/02—Preservation of living parts
    • A01N1/0236—Mechanical aspects
    • A01N1/0263—Non-refrigerated containers specially adapted for transporting or storing living parts whilst preserving, e.g. cool boxes, blood bags or "straws" for cryopreservation
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L9/00—Supporting devices; Holding devices
    • B01L9/52—Supports specially adapted for flat sample carriers, e.g. for plates, slides, chips
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/18—Transport of container or devices
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/04—Closures and closing means
    • B01L2300/041—Connecting closures to device or container
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/04—Closures and closing means
    • B01L2300/041—Connecting closures to device or container
    • B01L2300/043—Hinged closures
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/06—Auxiliary integrated devices, integrated components
    • B01L2300/0609—Holders integrated in container to position an object
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0809—Geometry, shape and general structure rectangular shaped
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0887—Laminated structure

Definitions

  • the present disclosure relates to the field of biotechnology, and in particular to a biological sheet fixing device.
  • Biological sheets having three-dimensional structures including but not limited to cell membranes, physiological sections, skin samples, etc., are research hotspots in the field of biological tissue engineering in recent years. Taking cell membranes as an example, it has a wide range of applications in the treatment of skin, cornea, heart, periodontal and other related diseases.
  • At least one embodiment of the present disclosure provides a biological sheet fixing device, the biological sheet fixing device comprising:
  • a carrier layer configured to carry the biological sheet
  • a carrying portion configured to carry the carrying layer
  • a fixing portion configured to fix the carrier layer to the carrier portion.
  • the fixing portion is a pressing structure
  • the pressing structure is configured to be capable of holding the bearing layer without bearing when the pressing structure is movably abutted against the bearing portion. Part of a biological sheet.
  • At least the surface of the carrier layer facing the biosheet is made of a material that is tissue compatible with the biosheet.
  • the material of the carrier layer is selected from the group consisting of polyethylene, polypropylene, polyethylene glycol, polystyrene, nylon, polyacetal, polycarbonate, polyvinyl alcohol, polyethyleneimine, polysulfone, poly At least one of lactic acid, polymethacrylate, polyurethane, glass, ceramic, and dextran.
  • the carrier layer includes a body configured to carry the bio-sheet and an extension extending from the body, the extension extending beyond the carrier.
  • the shape of the extension is any one of a rectangle, a circle, an ellipse, a triangle, and a trapezoid.
  • At least one through hole is defined in the body.
  • the body is provided with a plurality of through holes, and the plurality of the through holes are arranged in an array.
  • the shape of the through hole is any one of a rectangle, a circle, an ellipse, a triangle, a trapezoid, a V, an M, and a W.
  • the biological sheet fixing device further comprises an elastic flap structure, and the bearing portion is foldably connected to the fixing portion by the elastic flap structure.
  • the biological sheet fixing device further includes a rotating portion, and the carrying portion is rotatably connected to the fixing portion by the rotating portion.
  • the rotating portion includes a rotating shaft and a shaft sleeve sleeved outside the rotating shaft.
  • the rotating portion includes a rotating shaft and at least two damper sheets fixed on the rotating shaft, a first shaft barrel and a second shaft barrel, and the first shaft barrel is fixedly connected with the fixing portion, a second shaft barrel fixedly coupled to the carrying portion, the first shaft barrel and the second shaft barrel abutting on both sides of the at least two damping sheets, and in the first shaft barrel and the The second shaft barrel is resisted by the damping of the at least two dampers when rotated about the rotating shaft, wherein the generated damping resistance is greater than the gravity of the fixing portion.
  • the biological sheet fixing device further includes a locking portion, the locking portion includes a matching buckle and a card slot, and the buckle is disposed on one of the fixing portion and the bearing portion The card slot is disposed in the other of the fixing portion and the carrying portion.
  • the biological sheet fixing device further comprises a water permeable and permeable cover film, the cover film being located on the biological sheet.
  • the tablet structure comprises a first pressing piece and a second pressing piece, so that when the pressing piece structure is pressed against the carrying portion, the first pressing piece and the second pressing piece are respectively pressed Hold on both sides of the load-bearing layer.
  • the tablet structure comprises a third pressing piece, and the third pressing piece is fixedly connected to a side of the first pressing piece and the second pressing piece away from the connecting side.
  • FIG. 1 is a schematic structural view of a biological sheet fixing device according to some embodiments of the present disclosure
  • FIG. 2 is a schematic structural view of a biological sheet fixing device according to further embodiments of the present disclosure.
  • Figure 3 is a schematic view showing the first direction of the biological sheet fixing device shown in Figure 2;
  • Figure 4 is a schematic view showing the second direction of the biological sheet fixing device shown in Figure 2;
  • FIG. 5 is a schematic structural view of a carrier layer and a biological sheet of a biological sheet fixing device according to some embodiments of the present disclosure
  • FIG. 6 is a schematic structural view of a biological sheet fixing device according to some embodiments of the present disclosure.
  • Figure 7 is a schematic view showing the first direction of the biological sheet fixing device shown in Figure 6;
  • FIG. 8 is a schematic structural view of a carrier layer having a through hole of a biological sheet fixing device according to some embodiments of the present disclosure
  • FIG. 9 is a structural schematic view of a carrier layer having a through hole and a bio-sheet according to a biological sheet fixing device according to some embodiments of the present disclosure.
  • a particular device when it is described that a particular device is located between the first device and the second device, there may be intervening devices between the particular device and the first device or the second device, or there may be no intervening devices.
  • that particular device can be directly connected to the other device without intervening devices, or without intervening devices directly connected to the other devices.
  • the biological sheet fixing device of the present disclosure is suitable for preservation or transportation of a biological sheet, prevents wrinkles or tears of the biological sheet, and maintains the integrity and biological activity of the biological sheet.
  • the biological sheet fixing device includes a carrier layer 2, a carrier portion 4 and a fixing portion 15, wherein the carrier layer 2 is configured to be carried
  • the bio-sheet 1 the carrier portion 4 is configured to carry the carrier layer 2
  • the fixing portion 15 is configured to fix the carrier layer 2 to the carrier portion 4.
  • the carrier layer 2 may be selected from structures and/or materials suitable for attaching and flattening the bio-sheet 1.
  • the carrier layer 2 may adopt a carrier film, the carrier film itself has good flexibility and tissue compatibility, so that the biological sheet 1 carried thereon can be prevented from falling off, wrinkles and/or Was torn.
  • the biological sheet fixing device realizes that the biological sheet 1 is carried on the carrying portion 4 together by fixing the bearing layer 2 on the carrying portion 4 and carrying the biological sheet 1 on the carrying layer 2. / or transport, thereby facilitating the preservation and / or transportation of the biological sheet 1, and effectively avoid wrinkles or tears when the bio-sheet 1 is preserved or transported.
  • the attachment of the carrier layer 2 relative to the carrier 4 may be detachable.
  • the fixing portion 15 may be configured as one or more clamping mechanisms, at least two clips of the clamping mechanism being configured to respectively press the carrier layer 2 and the carrier portion 4, for example against the edge of the carrier layer 2 and the carrier portion 4
  • the edge or other selected location e.g., the selected location corresponds to the outer peripheral region of the carrier layer 2 to secure the carrier layer 2 and the carrier portion 4 together.
  • the clamping mechanism can be loosened.
  • the fixing portion 15 may be configured as one or more magnetic elements of opposite polarities respectively located on the opposite surfaces of the carrier layer 2, the carrier portion 4, and the two are fixed together by magnetic attraction.
  • the externally applied force is higher than the corresponding magnetic attraction force.
  • the fixing portion 15 may also be configured to include at least one sheet structure 17 configured to be movably coupled to the carrier portion 4 and pressed against the carrier portion 4, pressed There is no portion of the carrier layer 2 carrying the bio-sheet 1. By avoiding the pressing of the portion carrying the bio-sheet 1, possible contamination and damage to the loaded bio-sheet 1 can be avoided.
  • the tablet structure 17 is movably abutted against the carrier portion 4, and the tablet structure 17 is configured to simultaneously hold the carrier when the tablet structure 17 is pressed against the carrier portion 4. There is no portion of layer 2 carrying the bio-sheet 1.
  • the tablet structure 17 includes a first sheet 5 and a second sheet 6, which is pressed when the sheet structure 17 is pressed against the carrier 4. 5 and the second pressing piece 6 are respectively pressed against the carrier layer 2.
  • the sheet structure 17 can be more stably pressed against the carrier layer 2, preventing the carrier layer 2 from shaking.
  • the first tablet 5 and the second tablet 6 are respectively located on opposite sides of the carrier layer 2 for carrying the bio-sheet 1.
  • the first pressing piece 5 and the second pressing piece 6 are respectively pressed on both sides of the bearing layer 2, and the pressing is uniform, and the pressing effect is good.
  • the sheet structure 17 is not limited to such a structure.
  • the tablet structure 17 may include a different number of tablets; it may also be composed of a single sheet having an opening shape corresponding to the shape of the biological sheet 1.
  • a water permeable, breathable cover film 14 may be disposed over the opening, which cover film 14 may be secured to the outer periphery of the opening.
  • the cover film 14 is disposed to cover the biological sheet 1, the flat state of the biological sheet 1 can be maintained, the biological sheet 1 can be further fixed, and the biological sheet 1 can be exchanged for gas or nutrients through the cover film 14, and the biological sheet can be held.
  • the cover film 14 can be pre-fixed to the peripheral edge of the tablet structure 17, such that when the tablet structure 17 is pressed against the carrier portion 4, the cover film 14 covers the bio-sheet 1 without A special cover film 14 is arranged.
  • the fixing portion 15 is movably ("movably" intended to cover various activities, such as sliding, rotating, rolling, folding, etc.) connected to the carrying portion 4, for example, the pressing structure 17 relative to the carrying portion 4
  • the tablet structure 17 When sliding to the first position, the tablet structure 17 does not press against the carrier portion 4, and when the tablet structure 17 is slid relative to the carrier portion 4 to the second position, the tablet structure 17 is pressed against the carrier portion 4.
  • the tablet structure 17 can also be movably connected to the carrier portion 4 by other means, so that the tablet structure 17 can be pressed against the carrier portion 4 to press the portion of the carrier layer 2 that does not carry the biological sheet 1 and The sheet structure 17 is realized away from the carrier 4 to place the carrier layer 2 on the carrier 4 or to remove the carrier layer 2.
  • the tablet structure 17 can also be rotatably coupled to the carrier 4.
  • the biological sheet fixing device may further include a rotating portion 7, and the carrying portion 4 is rotationally coupled to the pressing structure 17 by the rotating portion 7.
  • the bearing portion 4 is rotatably connected to the pressing structure 17 by the rotating portion 7.
  • the pressing structure 17 can be pressed against the carrying portion 4 to press the bearing sheet 2 without carrying the biological sheet 1
  • the pressing structure 17 is away from the carrying portion 4 to place the carrying layer 2 on the carrying portion 4 or to remove the carrying layer 2.
  • the rotating portion 7 can also be an elastic flap structure, and the carrying portion 4 can be folded and connected with the pressing structure 17 by the elastic flap structure, so that the pressing structure 17 can be folded relative to the bearing portion 4 to the pressure.
  • the elastic flap structure can overcome the gravity of the tablet structure 17 to maintain the tablet structure 17 in the position relative to the carrier portion 4 for convenient access.
  • the rotating portion 7 includes a rotating shaft 9 and a barrel 8 sleeved outside the rotating shaft 9.
  • the rotating shaft 9 is fixedly connected with the bearing portion 4
  • the shaft barrel 8 is fixedly connected with the pressing piece structure 17, and the rotating shaft 9 and the shaft barrel 8 are relatively rotated to rotate the bearing portion 4 and the pressing piece structure 17 to press the pressing piece structure 17 against or away from the bearing. Department 4.
  • the rotating shaft 9 is fixedly coupled to the pressing structure 17, and the shaft barrel 8 is fixedly coupled to the carrying portion 4, and the bearing portion 4 and the pressing structure 17 can be relatively rotated.
  • the rotating portion 7 includes a rotating shaft and at least two damper sheets fixed to the rotating shaft, a first shaft barrel and a second shaft barrel, the first shaft barrel being fixedly connected with the pressing piece structure 17, and the second shaft barrel and
  • the bearing portion 4 is fixedly connected, and the first shaft barrel and the second shaft tube abut against the two sides of the at least two damping sheets, and are generated by the at least two damping sheets when the first shaft barrel and the second shaft barrel rotate around the rotating shaft
  • the resistance to damping, wherein the resulting damping has a greater resistance than the weight of the tablet structure 17.
  • the pressing structure 17 When the rotating portion 7 is rotated to a certain angle, the pressing structure 17 is away from the bearing portion 4, and the damping generated between the at least two damping sheets can prevent the pressing structure 17 from being automatically dropped at a certain angle by gravity, so that the bearing layer can be conveniently taken. 2 and a biological sheet 1 carried on the carrier layer 2.
  • the biological sheet 1 may be a cell sheet (cell membrane) having a three-dimensional structure, a tissue engineered cytoskeleton, or a single-layered plant tissue leaf, epidermal tissue, cell culture, or the like.
  • the bio-sheet 1 By carrying the bio-sheet 1 on the carrier layer 2, the bio-sheet 1 is carried by the carrier 4, the tablet structure 17 is movably connected to the carrier 4, and the tablet structure 17 is pressed against the carrier 4
  • the portion of the carrier layer 2 that does not carry the bio-sheet 1 prevents wrinkles or tears from occurring when the bio-sheet 1 is stored or transported.
  • the tablet structure 17 can also be conveniently at least partially disengaged from the carrier portion 4 to facilitate removal of the bio-sheet 1.
  • At least the surface of the carrier layer 2 facing the bio-sheet 1 is made of a material having tissue compatibility, and the term "tissue compatibility" as used herein refers to a structure constituting the biological sheet. Histocompatibility.
  • tissue compatibility refers to a structure constituting the biological sheet. Histocompatibility.
  • the material of the carrier layer 2 is selected from the group consisting of polyethylene, polypropylene, polyethylene glycol, polystyrene, nylon, polyacetal, polycarbonate, polyvinyl alcohol, polyethyleneimine, polysulfone, polylactic acid, poly At least one of methacrylate, polyurethane, glass, ceramic, and dextran.
  • the carrier layer 2 includes a body 16 configured to carry the bio-sheet 1 (ie, a projection of the bio-sheet 1 in a plane perpendicular to the plane of the carrier layer 2 is located within the body 16) and extends from the body 16.
  • the extension 3 when the carrier layer 2 is in place, the extension 3 extends beyond the carrier 4 (i.e., at least a portion of the extension 3 extends beyond the edge of the portion of the carrier 4), as shown in Figures 2 and 6.
  • the carrier layer 2 can be conveniently held to place the carrier layer 2 on the carrier portion 4 or to remove the carrier layer 2 from the carrier portion 4.
  • the shape of the extension 3 may be a regular shape, such as any of a rectangular shape (a rounded rectangle as shown in FIGS. 2, 4, and 6), a circle, an ellipse, a triangle, and a trapezoid, to facilitate Manufacturing.
  • a regular shape such as any of a rectangular shape (a rounded rectangle as shown in FIGS. 2, 4, and 6), a circle, an ellipse, a triangle, and a trapezoid, to facilitate Manufacturing.
  • At least one through hole 12 is opened in the body 16.
  • a plurality of through holes 12 are defined in the body 16.
  • the lower portion of the biological sheet fixing device is provided with a protective liquid or a culture liquid device.
  • the carrier layer 2 together with the biological sheet 1 is fixed on the biological sheet fixing device.
  • the biological sheet 1 can sufficiently contact the air or the protective liquid or the culture liquid through the plurality of through holes 12 to maintain the biological activity of the biological sheet 1.
  • a vacuum is formed between the biological sheet 1 and the carrier layer 2 to facilitate the picking up of the biological sheet 1 from the carrier layer 2.
  • the plurality of through holes 12 are arranged in an array.
  • the through hole 12 of the carrier layer 2 has a rectangular shape (a rounded rectangle as shown in FIGS. 8 and 9), a circle, an ellipse, a triangle, a trapezoid, and a V. Any of the shape, the M shape, and the W shape.
  • the shape of the through hole 12 is regular, easy to process, and the biological sheet 1 can be sufficiently brought into contact with air or a protective liquid or a culture solution.
  • the bio-sheet fixing device further includes a locking portion configured to be disposed on the carrier sheet 2 and the fixing portion 15 is moved to a pressed position relative to the carrier portion 4 At this time, the fixing portion 15 and the carrying portion 4 are locked in position, thereby avoiding displacement of the respective members, preventing loosening of the biological sheet fixing device, and avoiding the detachment of the biological sheet 1 thus caused.
  • the locking portion can take a variety of implementations.
  • the locking portion includes a snap fit 10 and a card slot 11 which are disposed on any one of the tablet structure 17 and the carrier portion 4, and the card slot 11 is disposed in the tablet structure 17. And the remaining one of the load-bearing portions 4.
  • the pressing structure 17 and the bearing portion 4 are locked by the engagement of the buckle 10 and the card slot 11 to press the pressing structure 17 against the bearing portion 4, and prevent the bearing layer 2 from moving out of the biological film during transportation.
  • the material fixing device or when the buckle 10 and the card slot 11 are in an open state, can take the carrier layer 2 and the biological sheet 1 carried on the carrier layer 2.
  • the bio-sheet securing device can further include a water permeable, breathable cover film 14 disposed on the bio-sheet 1.
  • the cover film 14 is provided, the flat state of the biological sheet 1 can be maintained, the biological sheet 1 can be further fixed, and the biological sheet 1 can be exchanged for gas or nutrients through the cover film 14, thereby maintaining the biological activity of the biological sheet 1.
  • the cover film 14 may be made of a high molecular polymer material having a microporous structure, such as a material using the carrier layer 2.
  • the tablet structure 17 is further balanced.
  • the third pressing piece 13 is included, and the third pressing piece 13 is fixedly coupled to the side of the first pressing piece 5 and the second pressing piece 6 away from the connecting side.
  • Other implementations of the tableting structure 17 have been given above.
  • the first pressing piece 5, the second pressing piece 6, the third pressing piece 13, and the rotating portion 7 shown in Fig. 6 constitute a closed frame, thereby facilitating the fixing of the cover film 14 and compared with the single piece pressing in the above
  • the structure of the sheet saves material and is simpler to manufacture.
  • the tablet structure 17 is pressed against the cover film 14 by being pressed against the carrier 4.
  • the carrier layer 2 carries the bio-sheet 1
  • the cover film 14 covers the bio-sheet 1
  • the tablet structure 17 presses the cover film 14 and the carrier layer 2
  • the bio-sheet 1 carries air or nutrients through the cover film 14 and the carrier layer 2.
  • the exchange, and the cover film 14 and the carrier layer 2 can also prevent the biological sheet 1 from moving during storage or transportation, thereby avoiding wrinkles or tears when the biological sheet 1 is stored or transported.

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Abstract

本公开实施例公开了一种生物片材固定装置，所述生物片材固定装置包括：承载层，配置成承载所述生物片材；承载部，配置成承载所述承载层；以及固定部，配置成将所述承载层固定在所述承载部。

Description

生物片材固定装置
相关申请
本申请要求保护在2018年2月13日提交的申请号为201810150492.0的中国专利申请的优先权，该申请的全部内容以引用的方式结合到本文中。
技术领域
本公开涉及生物技术领域，具体涉及一种生物片材固定装置。
背景技术
具有三维立体结构的生物片材，包括但不限于细胞膜片、生理切片、皮肤样本等，是近年来生物组织工程领域的研究热点。以细胞膜片为例，其在皮肤、角膜、心脏、牙周等相关疾病的治疗中具有广泛的应用。
公开内容
本公开的至少一个实施例提供一种生物片材固定装置，所述生物片材固定装置包括：
承载层，配置成承载所述生物片材；
承载部，配置成承载所述承载层；以及
固定部，配置成将所述承载层固定到所述承载部。
可选地，所述固定部为压片结构，所述压片结构配置成当所述压片结构可动地抵靠到所述承载部时，能够压持所述承载层中没有承载所述生物片材的部分。
可选地，至少所述承载层的面向所述生物片材的表面由与生物片材具有组织相容性的材料制成。
可选地，所述承载层的材质选自聚乙烯、聚丙烯、聚乙二醇、聚苯乙烯、尼龙、聚缩醛、聚碳酸酯、聚乙烯醇、聚乙烯亚胺、聚砜、聚乳酸、聚甲基丙烯酸酯、聚氨酯、玻璃、陶瓷、葡聚糖中的至少一种。
可选地，所述承载层包括配置成承载所述生物片材的本体和从所 述本体延伸的延伸部，所述延伸部超出所述承载部。
可选地，所述延伸部的形状为矩形、圆形、椭圆形、三角形和梯形中的任何一种。
可选地，所述本体上开设有至少一个通孔。
可选地，所述本体上开设有多个通孔，多个所述通孔呈阵列排布。
可选地，所述通孔的形状为矩形、圆形、椭圆形、三角形、梯形、V形、M形和W形中的任何一种。
可选地，该生物片材固定装置还包括弹性折片结构，所述承载部通过所述弹性折片结构与所述固定部可折叠连接。
可选地，该生物片材固定装置还包括转动部，所述承载部通过所述转动部与所述固定部转动连接。
可选地，所述转动部包括转轴以及套设在所述转轴外的轴筒。
可选地，所述转动部包括转轴以及固定在所述转轴上的至少两个阻尼片、第一轴筒和第二轴筒，所述第一轴筒与所述固定部固定连接，所述第二轴筒与所述承载部固定连接，所述第一轴筒和所述第二轴筒抵靠在所述至少两个阻尼片的两侧，并在所述第一轴筒和所述第二轴筒绕所述转轴转动时受到所述至少两个阻尼片所产生阻尼的阻力，其中，产生的阻尼的阻力大于所述固定部的重力。
可选地，该生物片材固定装置还包括锁紧部，所述锁紧部包括相互配合的卡扣和卡槽，所述卡扣设置在所述固定部和所述承载部中的一者，所述卡槽设置在所述固定部和所述承载部中的另一者。
可选地，该生物片材固定装置还包括透水透气的覆盖膜，所述覆盖膜位于所述生物片材上。
可选地，所述压片结构包括第一压片和第二压片，使得当所述压片结构压靠到承载部上时，所述第一压片和所述第二压片分别压持在承载层的两侧。
可选地，所述压片结构包括第三压片，所述第三压片固定连接到所述第一压片和所述第二压片的远离连接侧的一侧上。
附图说明
将在下文参照附图描述本公开的示范性实施例的特征、优势以及技术和工业意义，在附图中，相同附图标记指代相同的元件，并且其 中：
图1为根据本公开一些实施例的生物片材固定装置的结构示意图；
图2为根据本公开另一些实施例的生物片材固定装置的结构示意图；
图3为图2所示的生物片材固定装置的第一方向的示意图；
图4为图2所示的生物片材固定装置的第二方向的示意图；
图5为根据本公开一些实施例的生物片材固定装置的承载层和生物片材的结构示意图；
图6为根据本公开再一些实施例的生物片材固定装置的结构示意图；
图7为图6所示的生物片材固定装置的第一方向的示意图；
图8为根据本公开一些实施例的生物片材固定装置的具有通孔的承载层的结构示意图；
图9为根据本公开一些实施例的生物片材固定装置的具有通孔的承载层和生物片材的结构示意图。
具体实施方式
下面结合附图和具体实施例对本公开的实施例作进一步详细描述，但不作为对本公开的限定。以下说明旨在使得本领域技术人员能够理解并实现本公开。下文中具体实施例或应用的描述仅仅作为示例提供。对于各个实施例的各种修改对于本领域技术人员来说容易想到，以及本文中定义的主要原理可以应用于其他实施例和应用而不偏离本公开的精神和范围。因此，本公开并不意图限于所示的实施例，而是要求于其中公开的原理和特征相符的、由权利要求书限定的可能范围。虽然对各个实施例进行分别的说明，不同实施例中的特征和要素可以互相组合，组合方式并不限于各个独立的实施例。
本公开中使用的“第一”、“第二”以及类似的词语并不表示任何顺序、数量或者重要性，而只是用来区分不同的部分。“包括”或者“包含”等类似的词语意指在该词前的要素涵盖在该词后列举的要素，并不排除也涵盖其他要素的可能。“上”、“下”、“左”、“右”等仅用于表示相对位置关系，当被描述对象的绝对位置改变后，则该相对位置关系也可能相应地改变。本公开中使用的表述“A和/或B” 包含A而非B、B而非A以及A并且B中的任何一种，使用的表述“A、B和/或C”包含A、B和C三者中的任何一种或任何若干种的组合。
在本公开中，当描述到特定器件位于第一器件和第二器件之间时，在该特定器件与第一器件或第二器件之间可以存在居间器件，也可以不存在居间器件。当描述到特定器件连接其它器件时，该特定器件可以与所述其它器件直接连接而不具有居间器件，也可以不与所述其它器件直接连接而具有居间器件。
本公开使用的所有术语(包括技术术语或者科学术语)与本公开所属领域的普通技术人员理解的含义相同，除非另外特别定义。还应当理解，在诸如通用字典中定义的术语应当被解释为具有与它们在相关技术的上下文中的含义相一致的含义，而不应用理想化或极度形式化的意义来解释，除非这里明确地这样定义。
对于相关领域普通技术人员已知的技术、方法和设备可能不作详细讨论，但在适当情况下，所述技术、方法和设备应当被视为说明书的一部分。
本公开的生物片材固定装置，适合生物片材的保存或运输，防止生物片材发生褶皱或撕裂，保持生物片材的完整性和生物活性。
图1为根据本公开一些实施例的生物片材固定装置的结构示意图，如图1所示，生物片材固定装置包括承载层2、承载部4和固定部15，其中承载层2配置成承载生物片材1，承载部4配置成承载所述承载层2，以及固定部15配置成将承载层2固定在承载部4。
例如，所述承载层2可以选用适于附着和展平生物片材1的结构和/或材质。可选地但非限制性的，所述承载层2可以采用承载膜，承载膜自身良好的柔性和组织相容性，使得能够防止承载在其上的生物片材1脱落、发生褶皱和/或被撕裂。
本公开实施例提供的生物片材固定装置，通过将承载层2固定在承载部4并在承载层2上承载生物片材1，实现了将生物片材1承载在承载部4上一起保存和/或运输，从而便利生物片材1的保存和/或运输，并能有效地避免生物片材1保存或运输时发生褶皱或被撕裂。
在本公开的一些实施例中，承载层2相对于承载部4的固定可以是可拆卸的。
例如，固定部15可以构造为一个或多个夹持机构，夹持机构的至 少两个夹片配置成分别抵压承载层2和承载部4，例如抵压承载层2的边缘和承载部4的边缘或其它所选择的位置(例如所选择的位置对应于承载层2的外周缘区域)，从而将承载层2和承载部4固定在一起。在更换承载层2或承载部4时，松开夹持机构即可。
例如，固定部15可以构造为一个或多个分别位于承载层2、承载部4相对表面的极性相反的磁性元件，通过磁性吸引力将二者固定在一起。在更换承载层2或承载部4时，外部施加的力高于对应的磁性吸引力即可。
在本公开的一些实施例中，固定部15还可构造为包括至少一个压片结构17，压片结构17配置成可动地连接到承载部4，并压靠在承载部4上，压持所述承载层2中没有承载生物片材1的部分。通过避开对承载生物片材1的部分的压靠，可以避免对被承载的生物片材1的可能的污染和损害。
例如，如图2、3所示，压片结构17可动地抵靠到承载部4上，并且压片结构17配置成当压片结构17压靠在承载部4上时能够同时压持承载层2中没有承载生物片材1的部分。
在本公开的一些实施例中，如图2所示，压片结构17包括第一压片5和第二压片6，当压片结构17压靠到承载部4上时，第一压片5和第二压片6分别压持在承载层2上。通过设置分离的两个压片，能够使压片结构17更稳定地压持在承载层2上，防止承载层2晃动。
在本公开的一些实施例中，第一压片5和第二压片6分别位于用于承载生物片材1的承载层2的两侧。第一压片5和第二压片6分别压持在承载层2的两侧，压持均匀，压持效果好。
本领域技术人员容易理解，虽然图2中示出了由第一压片5和第二压片6构成的压片结构17，但压片结构17不限于这样的结构。例如，压片结构17可以包括不同数量的压片；也可以由单片压片构成，该单片压片具有与生物片材1的形状对应的开口形状。
在一些实施例中，开口上可以布置有透水透气的覆盖膜14，该覆盖膜14可以固定到开口的外周缘。设置覆盖膜14以覆盖生物片材1，可以保持生物片材1的平展状态，进一步固定生物片材1，还可以使生物片材1通过覆盖膜14进行气体或营养物质的交换，保持生物片材1的生物活性。
在一些实施例中，可以将覆盖膜14预先固定在压片结构17的开口周缘，从而压片结构17压靠在承载部4上时，覆盖膜14就恰好覆盖住生物片材1，而无需专门的覆盖膜14布置步骤。
其中，固定部15可动地(“可动地”旨在涵盖各种活动方式，例如滑动、转动、滚动、折叠等)连接到承载部4，例如可以是：压片结构17相对于承载部4滑动到第一位置时，压片结构17不压靠承载部4，压片结构17相对于承载部4滑动到第二位置时，压片结构17压靠在承载部4上。当然，压片结构17还可以通过其他的方式可动地连接到承载部4，以实现压片结构17能够压靠承载部4而压持承载层2中没有承载生物片材1的部分，以及实现压片结构17远离承载部4以在承载部4上放置承载层2或取走承载层2。
在一些实施例中，压片结构17还可以可转动地连接到承载部4。参考图2，该生物片材固定装置还可以包括转动部7，承载部4通过转动部7与压片结构17实现转动连接。承载部4通过转动部7与压片结构17实现转动连接，当转动部7转动到第一位置时，压片结构17能够压靠承载部4而压持承载层2中没有承载生物片材1的部分，当转动部7转动到第二位置时，压片结构17远离承载部4以在承载部4上放置承载层2或取走承载层2。
在一些实施例中，转动部7也可以为弹性折片结构，承载部4通过弹性折片结构与压片结构17实现可折叠连接，如此能够将压片结构17相对于承载部4折叠到压靠位置，并且在压片结构17相对于承载部4远离到一位置时，弹性折片结构能够克服压片结构17的重力使压片结构17相对于承载部4保持在该位置，方便取放承载层2和承载在承载层2上的生物片材1。
在一些实施例中，参考图4，转动部7包括转轴9以及套设在转轴9外的轴筒8。转轴9与承载部4固定连接，轴筒8与压片结构17固定连接，转轴9与轴筒8相对转动使承载部4与压片结构17相对转动以使压片结构17压靠或远离承载部4。
在一些实施例中，转轴9与压片结构17固定连接，轴筒8与承载部4固定连接，也可以实现承载部4与压片结构17相对转动。
在一些实施例中，转动部7包括转轴以及固定在转轴上的至少两个阻尼片、第一轴筒和第二轴筒，第一轴筒与压片结构17固定连接， 第二轴筒与承载部4固定连接，第一轴筒和第二轴筒抵靠在至少两个阻尼片的两侧，并在第一轴筒和第二轴筒绕转轴转动时受到至少两个阻尼片所产生阻尼的阻力，其中，产生的阻尼的阻力大于压片结构17的重力。转动部7转动到一定角度时，压片结构17远离承载部4，至少两个阻尼片之间产生的阻尼可以阻止压片结构17依靠重力自动下落而保持在该一定角度，方便取放承载层2和承载在承载层2上的生物片材1。
在本公开的一些实施例中，生物片材1可以是具有三维立体结构的细胞片层(细胞膜片)、组织工程细胞骨架，或单层结构的植物组织叶片、表皮组织、细胞培养物等。
通过在承载层2上承载生物片材1，利用承载部4承载生物片材1，压片结构17可动地连接到承载部4，在压片结构17经由压靠在承载部4上而压持承载层2中没有承载生物片材1的部分以避免生物片材1保存或运输时发生褶皱或被撕裂。压片结构17也可以方便地与承载部4至少部分地脱开，从而便利生物片材1的取出。
在本公开的一些实施例中，至少承载层2的面向生物片材1的表面由具有组织相容性的材料制成，此处所称的组织相容性指的是与构成生物片材的结构的组织相容性。当生物片材1承载在承载层2上后，生物片材1贴附在承载层2的具有组织相容性的表面上，避免生物片材1与承载层2之间的运动。进而，当承载层2承载生物片材1时，能够防止在运输过程中生物片材1与生物片材固定装置相互碰撞而损坏，压片结构17不必接触生物片材1，避免接触造成生物片材1的损害。压持承载层2中没有承载生物片材1的部分可保证承载层2不与生物片材固定装置发生运动。
例如，承载层2的材料选自聚乙烯、聚丙烯、聚乙二醇、聚苯乙烯、尼龙、聚缩醛、聚碳酸酯、聚乙烯醇、聚乙烯亚胺、聚砜、聚乳酸、聚甲基丙烯酸酯、聚氨酯、玻璃、陶瓷、葡聚糖中的至少一种。
参考图5，在一些实施例中，承载层2包括配置成承载生物片材1的本体16(即生物片材1在垂直于承载层2所在平面的投影位于本体16内)和从本体16延伸的延伸部3，承载层2就位时，延伸部3超出承载部4(即延伸部3的至少部分伸出到承载部4的部分边缘以外)，如图2和图6所示。从而，当压片结构17不压靠在承载部4上时，可 以方便地夹持承载层2，以在承载部4上放置承载层2或者从承载部4上取走承载层2。
例如，延伸部3的形状可以为规则形状，例如为矩形(如图2、图4和图6所示的圆角矩形)、圆形、椭圆形、三角形和梯形中的任何一种，以便于加工制造。
在本公开的一些实施例中，为了使生物片材1能够与空气或保护液或培养液充分接触，本体16上开设有至少一个通孔12。参考图8和图9，本体16上开设有多个通孔12。通常情况下，生物片材固定装置的下部会设置有保护液或培养液装置，当生物片材1承载在承载层2上，承载层2连同生物片材1固定在生物片材固定装置上时，生物片材1通过多个通孔12可以与空气或保护液或培养液充分接触，保持生物片材1的生物活性。另外，当承载层2上开设多个通孔12后，可以避免生物片材1与承载层2之间形成真空，以方便从承载层2上取放生物片材1。
在一些实施例中，参考图8和图9，为了使生物片材1与空气或保护液或培养液的接触更加充分和均匀，多个通孔12呈阵列排布。
在本公开实施例的生物片材固定装置中，承载层2的通孔12的形状为矩形(如图8和图9所示的圆角矩形)、圆形、椭圆形、三角形、梯形、V形、M形和W形中的任何一种。通孔12的形状规则，容易加工制作，而且能够使生物片材1与空气或保护液或培养液充分接触。
在一些实施例中，该生物片材固定装置还包括锁紧部，锁紧部配置成：在生物片材1设置在承载层2上并且固定部15活动到相对于承载部4处于压靠位置时，将固定部15和承载部4锁定就位，从而避免各个构件的移位，防止生物片材固定装置的松动，避免由此造成的生物片材1的脱离。
锁紧部可以采用各种实现方式。作为示例，参考图4，锁紧部包括相互配合的卡扣10和卡槽11，卡扣10设置在压片结构17和承载部4中的任何一个上，卡槽11设置在压片结构17和承载部4中的剩余一个上。压片结构17和承载部4通过卡扣10和卡槽11的配合，实现锁紧以使压片结构17压靠在承载部4上，并防止运输过程中承载层2发生运动而脱离生物片材固定装置，或当卡扣10和卡槽11处于打开状态时，可以取放承载层2和承载在承载层2上的生物片材1。
参考图6和图7，在一些实施例中，该生物片材固定装置还可以包括透水透气的覆盖膜14，覆盖膜14设置在生物片材1上。设置覆盖膜14，可以保持生物片材1的平展状态，进一步固定生物片材1，还可以使生物片材1通过覆盖膜14进行气体或营养物质的交换，保持生物片材1的生物活性。
在一些实施例中，覆盖膜14可以采用具有微孔结构的高分子聚合物材料制成，例如采用承载层2的材料。
参考图6和图7，在一些实施例中，为了使第一压片5和第二压片6压持承载层2上待承载生物片材1的部分的力更加均衡，压片结构17还包括第三压片13，第三压片13固定连接到第一压片5和第二压片6的远离连接侧的一侧上。上文中已经给出了压片结构17的其他实现方式。图6中示出的第一压片5、第二压片6、第三压片13和转动部7组成闭合框架，由此更便利覆盖膜14的固定，并且相较上文中的单片压片的结构节省了材料，制造更简单。
参考图6和图7，压片结构17经由压靠在承载部4上而压持覆盖膜14。承载层2承载生物片材1，覆盖膜14覆盖生物片材1，压片结构17压持覆盖膜14和承载层2，生物片材1通过覆盖膜14和承载层2进行空气或营养物质的交换，并且覆盖膜14和承载层2还可以防止生物片材1在保存或运输时发生运动，从而避免生物片材1保存或运输时褶皱或撕裂。
需要说明的是，本公开使用了表达“一实施例”、“一个实施例”或“一些实施例”连同它们的派生词，这些表达和术语是指结合实施例描述的特定特征、结构或特性被包括在至少一个实施例中。在本公开各个地方出现的词组“在一个实施例中”不一定必须全都指同一个实施例。
虽然本主题是以特定于结构特征和/或方法动作的语言被描述的，但应当明白，在所附权利要求中限定的主题不是必须限于以上描述的具体特征或动作。相反，上面描述的具体的特征和动作是作为实施权利要求的示例性形式被公开的。显然，本领域的技术人员可以对本公开实施例进行各种改动和变型而不脱离本公开的精神和范围。这样，倘若本公开的这些修改和变型属于本公开权利要求及其等同技术的范围之内，则本公开也意图包含这些改动和变型在内。

Claims (17)

  1. 一种生物片材固定装置，包括：
    承载层，配置成承载生物片材；
    承载部，配置成承载所述承载层；以及
    固定部，配置成将所述承载层固定到所述承载部。
  2. 根据权利要去1所述的生物片材固定装置，其中，所述固定部为压片结构，所述压片结构配置成当所述压片结构可动地抵靠到所述承载部时，能够同时压持所述承载层中没有承载所述生物片材的部分。
  3. 根据权利要求1所述的生物片材固定装置，其中，至少所述承载层的面向所述生物片材的表面由与生物片材具有组织相容性的材料制成。
  4. 根据权利要求3所述的生物片材固定装置，其中，所述承载层的材质选自聚乙烯、聚丙烯、聚乙二醇、聚苯乙烯、尼龙、聚缩醛、聚碳酸酯、聚乙烯醇、聚乙烯亚胺、聚砜、聚乳酸、聚甲基丙烯酸酯、聚氨酯、玻璃、陶瓷、葡聚糖中的至少一种。
  5. 根据权利要求1所述的生物片材固定装置，其中，所述承载层包括配置成承载所述生物片材的本体和从所述本体延伸的延伸部，所述延伸部超出所述承载部。
  6. 根据权利要求5所述的生物片材固定装置，其中，所述延伸部的形状为矩形、圆形、椭圆形、三角形和梯形中的任何一种。
  7. 根据权利要求5所述的生物片材固定装置，其中，所述本体上开设有至少一个通孔。
  8. 根据权利要求7所述的生物片材固定装置，其中，所述本体上开设有多个通孔，多个所述通孔呈阵列排布。
  9. 根据权利要求7所述的生物片材固定装置，其中，所述通孔的形状为矩形、圆形、椭圆形、三角形、梯形、V形、M形和W形中的任何一种。
  10. 根据权利要求1所述的生物片材固定装置，其中，该生物片材固定装置还包括弹性折片结构，所述承载部通过所述弹性折片结构与所述固定部可折叠连接。
  11. 根据权利要求1所述的生物片材固定装置，其中，该生物片材 固定装置还包括转动部，所述承载部通过所述转动部与所述固定部实现转动连接。
  12. 根据权利要求11所述的生物片材固定装置，其中，所述转动部包括转轴以及套设在所述转轴外的轴筒。
  13. 根据权利要求11所述的生物片材固定装置，其中，所述转动部包括转轴以及固定在所述转轴上的至少两个阻尼片、第一轴筒和第二轴筒，所述第一轴筒与所述固定部固定连接，所述第二轴筒与所述承载部固定连接，所述第一轴筒和所述第二轴筒抵靠在所述至少两个阻尼片的两侧，并在所述第一轴筒和所述第二轴筒绕所述转轴转动时受到所述至少两个阻尼片所产生阻尼的阻力，其中，产生的阻尼的阻力大于所述固定部的重力。
  14. 根据权利要求1或11所述的生物片材固定装置，其中，该生物片材固定装置还包括锁紧部，所述锁紧部包括相互配合的卡扣和卡槽，所述卡扣设置在所述固定部和所述承载部中的一者，所述卡槽设置在所述固定部和所述承载部中的另一者。
  15. 根据权利要求1所述的生物片材固定装置，其中，该生物片材固定装置还包括透水透气的覆盖膜，所述覆盖膜位于所述生物片材上。
  16. 根据权利要求2所述的生物片材固定装置，其中，所述压片结构包括第一压片和第二压片，使得当所述压片结构压靠到承载部上时，所述第一压片和所述第二压片分别压持在承载层的两侧。
  17. 根据权利要求16所述的生物片材固定装置，其中，所述压片结构包括第三压片，所述第三压片固定连接到所述第一压片和所述第二压片的远离连接侧的一侧上。
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Publication number Priority date Publication date Assignee Title
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Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2003077794A2 (en) * 2002-03-14 2003-09-25 Tissuetech, Inc. Amniotic membrane covering for a tissue surface and devices facilitating fastening of membranes
CN101177184A (zh) * 2006-11-08 2008-05-14 王海滨 标本夹及其使用方法
CN102807965A (zh) * 2012-08-28 2012-12-05 陕西瑞盛生物科技有限公司 一种组织工程角膜的制备方法及其装置
CN203455203U (zh) * 2013-07-12 2014-02-26 张丽 病理切片固定装置
CN203811892U (zh) * 2014-01-20 2014-09-03 北京农学院 一种适用于倒置显微镜观察的玻片装置
CN106092707A (zh) * 2016-07-19 2016-11-09 南昌德漫多科技有限公司 一种用于对同一组织切片进行多次免疫染色的装置和方法
CN108353890A (zh) * 2018-02-13 2018-08-03 京东方科技集团股份有限公司 一种生物片材固定装置

Family Cites Families (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3556633A (en) * 1969-01-17 1971-01-19 Winifred Liu Mutschmann Specimen carrying slide with runoff trough
ES2265057T3 (es) * 2001-11-01 2007-02-01 Integrated Biosystems Inc. Sistemas y procedimientos para congelar, almacenar y descongelar material biofarmaceutico.
CA2489412A1 (en) * 2002-07-16 2004-01-22 Ventana Medical Systems, Inc. Tray for automated histochemical processing
WO2004083824A1 (en) * 2003-03-20 2004-09-30 Dakocytomation Denmark A/S System for establishing a sample cover on a substrate
NO20045625D0 (no) * 2004-12-23 2004-12-23 Torstein Ljungmann Holdeanordning for objektglass med vevsprover
CN101017174A (zh) * 2005-10-04 2007-08-15 米利波尔公司 蛋白质微阵列载物片
CN2831170Y (zh) * 2005-11-14 2006-10-25 麦克奥迪实业集团有限公司 显微镜载玻片夹联动装置
EP2214824A4 (en) * 2007-11-28 2015-08-19 Smart Tube Inc DEVICES, SYSTEMS AND METHODS FOR DETECTING, STIMULATING, STABILIZING AND ANALYZING A BIOLOGICAL SAMPLE
US8062861B2 (en) * 2008-02-21 2011-11-22 George Mason Intellectual Properties, Inc. Tissue sample preprocessing methods and devices
CN201163310Y (zh) * 2008-03-17 2008-12-10 关向宏 细胞学载玻片
CN102707420A (zh) * 2012-06-23 2012-10-03 南通江中光电有限公司 一种显微镜的标本夹
CN105413786B (zh) * 2015-12-21 2017-12-22 安徽农业大学 一种倾斜角度可调的恒温试管架
DE102016225885B4 (de) * 2016-12-21 2023-12-21 Prime23 GmbH Vorrichtung und Verfahren zum Benetzen von biologischem Material

Patent Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2003077794A2 (en) * 2002-03-14 2003-09-25 Tissuetech, Inc. Amniotic membrane covering for a tissue surface and devices facilitating fastening of membranes
CN101177184A (zh) * 2006-11-08 2008-05-14 王海滨 标本夹及其使用方法
CN102807965A (zh) * 2012-08-28 2012-12-05 陕西瑞盛生物科技有限公司 一种组织工程角膜的制备方法及其装置
CN203455203U (zh) * 2013-07-12 2014-02-26 张丽 病理切片固定装置
CN203811892U (zh) * 2014-01-20 2014-09-03 北京农学院 一种适用于倒置显微镜观察的玻片装置
CN106092707A (zh) * 2016-07-19 2016-11-09 南昌德漫多科技有限公司 一种用于对同一组织切片进行多次免疫染色的装置和方法
CN108353890A (zh) * 2018-02-13 2018-08-03 京东方科技集团股份有限公司 一种生物片材固定装置

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