WO2019119664A1 - 一种全面抗氧化保健食品及制备方法 - Google Patents

一种全面抗氧化保健食品及制备方法 Download PDF

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Publication number
WO2019119664A1
WO2019119664A1 PCT/CN2018/079972 CN2018079972W WO2019119664A1 WO 2019119664 A1 WO2019119664 A1 WO 2019119664A1 CN 2018079972 W CN2018079972 W CN 2018079972W WO 2019119664 A1 WO2019119664 A1 WO 2019119664A1
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extract
green tea
grape seed
health food
polygonum cuspidatum
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PCT/CN2018/079972
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English (en)
French (fr)
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王亚农
武婕
潘杰彤
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苏州诺衡生命科技有限公司
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Publication of WO2019119664A1 publication Critical patent/WO2019119664A1/zh

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    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A23L33/105—Plant extracts, their artificial duplicates or their derivatives
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/70—Polygonaceae (Buckwheat family), e.g. spineflower or dock
    • A61K36/704—Polygonum, e.g. knotweed
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/75—Rutaceae (Rue family)
    • A61K36/752—Citrus, e.g. lime, orange or lemon
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/82—Theaceae (Tea family), e.g. camellia
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/87—Vitaceae or Ampelidaceae (Vine or Grape family), e.g. wine grapes, muscadine or peppervine
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/88—Liliopsida (monocotyledons)
    • A61K36/886—Aloeaceae (Aloe family), e.g. aloe vera
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/88—Liliopsida (monocotyledons)
    • A61K36/896—Liliaceae (Lily family), e.g. daylily, plantain lily, Hyacinth or narcissus
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/88—Liliopsida (monocotyledons)
    • A61K36/906—Zingiberaceae (Ginger family)
    • A61K36/9066—Curcuma, e.g. common turmeric, East Indian arrowroot or mango ginger
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs

Definitions

  • the invention belongs to the technical field of health care products, and particularly relates to a formula and a preparation method of a comprehensive antioxidant health food.
  • Free radicals are intermediate metabolites of various biochemical reactions in human life activities. They have high chemical activity and are effective defense systems. If they cannot maintain a certain level, they will affect the life activities of the body. Auto-oxidation, enzymatic oxidation, respiratory incorporation, drug metabolism, and exposure to external electromagnetic radiation, tobacco smoking, and air pollution in the body can lead to excessive production of free radicals in the body. However, if too much free radicals are produced and cannot be removed in time, it will attack the living macromolecules and various organelles in the body, causing various damages at the molecular level, cell level and tissue and organ levels, and accelerating the aging process of the body. And induce various diseases.
  • Excessive free radicals in the body can weaken the resistance of cells, make the body susceptible to bacterial and pathogenic infections; produce chemical substances that destroy cells, form carcinogens; hinder cell growth and repair; destroy DNA, cell membranes, organelles, etc.; destroy proteins and enzymes It causes inflammation and aging; destroys fat structure, causes lipid peroxidation, causes a series of cardiovascular and cerebrovascular diseases such as atherosclerosis; destroys carbohydrates, degrades hyaluronic acid, and causes arthritis.
  • Active oxygen (ROS) in the human body mainly includes peroxy radical (ROO ⁇ ), hydroxyl radical ( ⁇ OH), superoxide anion (O 2 - ⁇ ), singlet oxygen ( 1 O 2 ), peroxynitrite Base (ONOO - ) and so on.
  • ROO ⁇ peroxy radical
  • ⁇ OH hydroxyl radical
  • O 2 - ⁇ superoxide anion
  • 1 O 2 singlet oxygen
  • ONOO - peroxynitrite Base
  • the present invention provides a formula and a preparation method for a comprehensive antioxidant health food.
  • a comprehensive antioxidant health food mainly consists of the following components by mass percentage: grape seed extract 10% to 60%, Polygonum cuspidatum extract 0.05% to 30%, turmeric extract 0.05% to 30%, green tea extract 0.05% to 30%, aloe vera gel lyophilized powder 200:10.05% to 30%, citrus bioflavonoids 0.05% to 30%, wherein the grape seed extract polyphenol content ⁇ 85%, the turmeric extract The content of curcumin in the product is ⁇ 90%, the content of resveratrol in the extract of Polygonum cuspidatum is ⁇ 45%, and the content of tea polyphenol in the green tea extract is ⁇ 90%.
  • the food product further comprises 10% to 50% of microcrystalline cellulose, 5% to 40% of magnesium stearate, and 2% to 35% of silicon dioxide.
  • the food is mainly composed of the following components in mass percentage: grape seed extract 20% to 60%, Polygonum cuspidatum extract 0.05% to 10%, turmeric extract 0.05% to 20%, green tea extract 0.05% to 20% %, aloe vera gel lyophilized powder 200:1 0.05% ⁇ 20%, citrus bioflavonoids 0.05% ⁇ 20%.
  • the food product is mainly composed of the following components in a mass percentage: grape seed extract 20% to 50%, Polygonum cuspidatum extract 0.05% to 5%, turmeric extract 0.05% to 10%, green tea extract 0.05% to 10% %, aloe vera gel lyophilized powder 200:1 0.05% ⁇ 10%, citrus bioflavonoids 0.05% ⁇ 10%.
  • the food product is mainly composed of the following components in mass percentage: grape seed extract 30% to 40%, Polygonum cuspidatum extract 0.05% to 3%, turmeric extract 0.05% to 5%, green tea extract 0.05% to 5 %, aloe vera gel lyophilized powder 200:1 0.05% ⁇ 5%, citrus bioflavonoids 0.05% ⁇ 5%.
  • the food product further comprises 20% to 40% of microcrystalline cellulose, 10% to 20% of magnesium stearate, and 5% to 20% of silicon dioxide.
  • the food product further comprises 30% to 40% of microcrystalline cellulose, 12% to 18% of magnesium stearate, and 10% to 18% of silicon dioxide.
  • the food product further comprises 35% to 40% of microcrystalline cellulose, 13% to 17% of magnesium stearate, and 10% to 17% of silicon dioxide.
  • the antioxidant comprises scavenging peroxy radicals, hydroxyl radicals, superoxide anions, singlet oxygen, peroxynitrite groups.
  • the method for preparing a comprehensive antioxidant health food product comprises the following steps,
  • auxiliary materials are added, auxiliary materials are added in S3, remixed, granulated, and dried to prepare a desired capsule dosage form or tablet.
  • the auxiliary materials are microcrystalline cellulose 10% to 50%, magnesium stearate 5%. ⁇ 40%, silica 2% to 35%.
  • the health care formula of the invention exerts a synergistic anti-oxidation effect through a combination of various antioxidants, and obtains evidence of the ability to remove different free radicals through a reliable scientific method, and can comprehensively alleviate oxidation.
  • the stress response damages the body and protects the cells, tissues and organs of the body, thereby achieving the effect of delaying aging and preventing diseases.
  • the technical solution of the present invention is specifically described below in conjunction with the examples.
  • the present invention discloses a health food having a comprehensive antioxidant function, which includes scavenging peroxy radicals (ROO ⁇ ) and hydroxyl radicals ( ⁇ OH).
  • ROO ⁇ peroxy radicals
  • ⁇ OH hydroxyl radicals
  • the polyphenol content in grape seed extract is ⁇ 85%
  • the curcumin content in turmeric extract is ⁇ 90%
  • the resveratrol content in Polygonum cuspidatum extract is ⁇ 45%
  • the tea polyphenol content in green tea extract is ⁇ 90 %.
  • a health food with comprehensive antioxidant function which is made of the following raw materials by mass percentage:
  • the raw material is passed through an 80 mesh sieve, and the sieved raw materials are mixed in an equal amount, added with an auxiliary material, granulated, dried, and prepared into a capsule form or tablet according to a conventional process.
  • a health food with comprehensive antioxidant function which is made of the following raw materials by mass percentage:
  • the raw material is passed through an 80 mesh sieve, and the sieved raw materials are mixed in an equal amount, added with an auxiliary material, granulated, dried, and prepared into a capsule form or tablet according to a conventional process.
  • a health food with comprehensive antioxidant function which is made of the following raw materials by mass percentage:
  • the raw material is passed through an 80 mesh sieve, and the sieved raw materials are mixed in an equal amount, added with an auxiliary material, granulated, dried, and prepared into a capsule form or tablet according to a conventional process.
  • a health food with comprehensive antioxidant function which is made of the following raw materials by mass percentage:
  • the raw material is passed through an 80 mesh sieve, and the sieved raw materials are mixed in an equal amount, added with an auxiliary material, granulated, dried, and prepared into a capsule form or tablet according to a conventional process.
  • a health food with comprehensive antioxidant function which is made of the following raw materials by mass percentage:
  • the raw material is passed through an 80 mesh sieve, and the sieved raw materials are mixed in an equal amount, added with an auxiliary material, granulated, dried, and prepared into a capsule form or tablet according to a conventional process.
  • a health food with comprehensive antioxidant function which is made of the following raw materials by mass percentage:
  • the raw material is passed through an 80 mesh sieve, and the sieved raw materials are mixed in an equal amount, added with an auxiliary material, granulated, dried, and prepared into a capsule form or tablet according to a conventional process.
  • the auxiliary material in the invention is similar to the preparation of the capsule or the tablet in the prior art, and other diluents, lubricants, glidants and the like required for capsules and tablets can be used according to specific needs, and no specific refinement is performed here. .
  • the invention also discloses an evaluation and test of the inventions of the comprehensive antioxidant health food examples 1 to 6 based on the ORAC 5.0 analysis technology and the CAA cell antioxidant activity evaluation method.
  • the invention has verified the function of a health food with comprehensive anti-oxidation function, capable of scavenging peroxy radical (ROO ⁇ ), hydroxyl radical ( ⁇ OH), superoxide anion (O 2 - ⁇ ), singlet oxygen ( 1 O 2 ), peroxynitrite group (ONOO - ).
  • ORAC 5.0 analysis technology is: adding a detector (fluorescent probe) and a reactive oxygen/reactive nitrogen (ROS/RNS) generator (substrate) to the sample (antioxidant), and ROS/RNS attacks the fluorescent probe to produce Fluorescence changes (increased or weakened fluorescence), once acted upon by antioxidants, the antioxidants in the system competitively inhibit the detection agent from reacting with the substrate, thereby inhibiting changes in fluorescence.
  • This method uses Trolox as a positive control to evaluate the ROS/RNS scavenging ability of the sample (antioxidant) in combination with changes in fluorescence intensity.
  • the principle is that the fluorescent probe DCFH-DA can pass through the cell membrane freely, enter the cell, and be hydrolyzed by the esterase in the cell to form DCFH, which cannot pass through the cell membrane freely, and thus is loaded into the cell.
  • reactive oxygen free radicals can oxidize DCFH without fluorescence to fluorescent DCF.
  • Quercetin was used as a positive control to evaluate the level of intracellular reactive oxygen species in the cells by detecting changes in the fluorescence intensity of DCF.
  • the free radical scavenging ability of the invented health food examples 1 to 6 was evaluated and tested by the two techniques.
  • the ORAC value peroxy radical scavenging ability
  • the HORAC value hydroxy radical scavenging ability
  • SORAC value (superoxide anion scavenging capacity) is 3600 ⁇ 5200 ⁇ mol TE / g
  • SOAC value single-line oxygen scavenging capacity
  • NORAC value peroxynitrite removal ability

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Abstract

一种全面抗氧化保健食品，主要由以下成分按质量百分比组成：葡萄籽提取物10％～60％，虎杖提取物0.05％～30％，姜黄提取物0.05％～30％，绿茶提取物0.05％～30％，芦荟凝胶冻干粉200:1 0.05％～30％，柑橘生物类黄酮0.05％～30％，其中所述葡萄籽提取物中多酚含量≥85％，所述姜黄提取物中姜黄素含量≥90％，所述虎杖提取物中白藜芦醇含量≥45％，所述绿茶提取物中茶多酚含量≥90％。该保健食品的制备方法包括按质量百分比称取原料、过70-90目筛进行筛选、混合、辅料添加与混合、制粒和干燥。

Description

一种全面抗氧化保健食品及制备方法 技术领域
本发明属于保健品技术领域，具体涉及一种全面抗氧化保健食品的配方及制备方法。
背景技术
自由基(Free radical)是人体生命活动中各种生化反应的中间代谢产物，具有高度的化学活性，是机体有效的防御系统，若不能维持一定水平则会影响机体的生命活动。生物体内自动氧化、酶促氧化、呼吸带入、药物代谢以及受到外界电磁辐射、吸食烟草、空气污染等都会导致机体内自由基的过量产生。但自由基产生过多而不能及时地清除，它就会攻击机体内的生命大分子物质及各种细胞器，造成机体在分子水平、细胞水平及组织器官水平的各种损伤，加速机体的衰老进程并诱发各种疾病。
体内自由基过量会削弱细胞的抵抗力，使机体易受细菌和病菌感染；产生破坏细胞的化学物质，形成致癌物质；阻碍细胞的生长修复；破坏DNA、细胞膜、细胞器等；破坏蛋白质和酶类，导致炎症和衰老；破坏脂肪结构，使脂质过氧化，引起动脉粥样硬化等一系列心脑血管疾病；破坏碳水化合物，使透明质酸降解，导致关节炎等。
人体内的活性氧(ROS)主要包括过氧自由基(ROO·)、羟基自由基(·OH)、超氧阴离子(O 2 －·)、单线态氧( 1O 2)、过氧亚硝酸基(ONOO －)等。
目前国内的抗氧化功能保健食品，并未具体对其能够清除哪种氧自由基进行确切研究及说明，抗氧化概念笼统且没有明确的评价指标。
发明内容
为了解决现有技术的不足，本发明提供了一种全面抗氧化保健食品的配方及制备方法。
本发明的目的通过以下技术方案来实现：
一种全面抗氧化保健食品，所述食品主要由以下成分按质量百分比组成：葡萄籽提取物10％～60％，虎杖提取物0.05％～30％，姜黄提取物0.05％～30％，绿茶提取物0.05％～30％，芦荟凝胶冻干粉200:10.05％～30％，柑橘生物类黄酮0.05％～30％，其中所述葡萄籽提取物中多酚含量≥85％，所述姜黄提取物中姜黄素含量≥90％，所述虎杖提取物中白藜芦醇含量≥45％，所述绿茶提取物中茶多酚含量≥90％。
优选地，所述食品中还包括微晶纤维素10％～50％，硬脂酸镁5％～40％，二氧化硅2％～35％。
优选地，所述食品主要由以下成分按质量百分比组成：葡萄籽提取物20％～60％，虎杖提取物0.05％～10％，姜黄提取物0.05％～20％，绿茶提取物0.05％～20％，芦荟凝胶冻干粉200:1 0.05％～20％，柑橘生物类黄酮0.05％～20％。
优选地，所述食品主要由以下成分按质量百分比组成：葡萄籽提取物20％～50％，虎杖提取物0.05％～5％，姜黄提取物0.05％～10％，绿茶提取物0.05％～10％，芦荟凝胶冻干粉200:1 0.05％～10％，柑橘生物类黄酮0.05％～10％。
优选地，所述食品主要由以下成分按质量百分比组成：葡萄籽提取物30％～40％，虎杖提取物0.05％～3％，姜黄提取物0.05％～5％，绿茶提取物0.05％～5％，芦荟凝胶冻干粉200:1 0.05％～5％，柑橘生物类黄酮0.05％～5％。
优选地，所述食品中还包括微晶纤维素20％～40％，硬脂酸镁10％～20％，二氧化硅5％～20％。
优选地，所述食品中还包括微晶纤维素30％～40％，硬脂酸镁12％～18％，二氧化硅10％～18％。
优选地，所述食品中还包括微晶纤维素35％～40％，硬脂酸镁13％～17％，二氧化硅10％～17％。
优选地，所述抗氧化包括清除过氧自由基、羟自由基、超氧阴离子、单线态氧、过氧亚硝酸基。
优选地，所述的一种全面抗氧化保健食品的制备方法，包括如下步骤，
S1、原料准备，按质量百分比称取原料：葡萄籽提取物10％～60％，虎杖提取物0.05％～30％，姜黄提取物0.05％～30％，绿茶提取物0.05％～30％，芦荟凝胶冻干粉200:1 0.05％～30％，柑橘生物类黄酮0.05％～30％，其中所述葡萄籽提取物中多酚含量≥85％，所述姜黄提取物中姜黄素含量≥90％，所述虎杖提取物中白藜芦醇含量≥45％，所述绿茶提取物中茶多酚含量≥90％；
S2、筛选，将准备好的原料并经过70-90目筛进行筛选，选出粒度均匀的原料待用；
S3、混合，将过筛后的原料按照等量递增的方法混合均匀；
S4、辅料添加，在S3中加入辅料，进行再次混合，制粒，干燥，制备成所需的胶囊剂型或片剂所述辅料为微晶纤维素10％～50％，硬脂酸镁5％～40％，二氧化硅2％～35％。
本发明的有益效果体现在：本发明中的保健配方通过多种抗氧化物的组合，发挥了协同抗氧化作用，并通过可靠的科学方法得到了清除不同自由基能力的佐证，可以全面缓解氧化应激反应对机体的损伤，保护机体的细胞、组织、器官，从而达到延缓衰老，预防疾病的功效。
具体实施方式
以下结合实施例具体阐述本发明的技术方案，本发明揭示了一种 具有全面抗氧化功能的保健食品，所述抗氧化能力包括清除过氧自由基(ROO·)、羟自由基(·OH)、超氧阴离子(O2－·)、单线态氧(1O2)、过氧亚硝酸基(ONOO－)的能力。
以下实施例中葡萄籽提取物中多酚含量≥85％，姜黄提取物中姜黄素含量≥90％，虎杖提取物中白藜芦醇含量≥45％，绿茶提取物中茶多酚含量≥90％。
实施例1
一种具有全面抗氧化功能的保健食品，按质量百分比用下述原料制成：
葡萄籽提取物97mg，虎杖提取物1.25mg，姜黄提取物1mg，绿茶提取物0.25mg，芦荟凝胶冻干粉200:1 0.25mg，柑橘生物类黄酮0.25mg，微晶纤维素120mg，硬脂酸镁45mg，二氧化硅35mg。将所述原料过80目筛，将过筛后的原料按照等量递增混合后，加入辅料，制粒，干燥，按照常规工艺制备成胶囊剂型或片剂。
实施例2
一种具有全面抗氧化功能的保健食品，按质量百分比用下述原料制成：
葡萄籽提取物97mg，虎杖提取物0.25mg，姜黄提取物1mg，绿茶提取物1.25mg，芦荟凝胶冻干粉200:1 0.25mg，柑橘生物类黄酮0.25mg，微晶纤维素110mg，硬脂酸镁55mg，二氧化硅35mg。将所述原料过80目筛，将过筛后的原料按照等量递增混合后，加入辅料，制粒，干燥，按照常规工艺制备成胶囊剂型或片剂。
实施例3
一种具有全面抗氧化功能的保健食品，按质量百分比用下述原料制成：
葡萄籽提取物97mg，虎杖提取物1mg，姜黄提取物0.25mg，绿茶提取物0.25mg，芦荟凝胶冻干粉200:1 0.25mg，柑橘生物类黄酮1.25mg，微晶纤维素110mg，硬脂酸镁45mg，二氧化硅45mg。将所述原料过80目筛，将过筛后的原料按照等量递增混合后，加入辅料，制粒，干燥，按照常规工艺制备成胶囊剂型或片剂。
实施例4
一种具有全面抗氧化功能的保健食品，按质量百分比用下述原料制成：
葡萄籽提取物95mg，虎杖提取物0.75mg，姜黄提取物0.25mg，绿茶提取物0.75mg，芦荟凝胶冻干粉200:1 1.25mg，柑橘生物类黄酮2mg，微晶纤维素130mg，硬脂酸镁35mg，二氧化硅35mg。将所述原料过80目筛，将过筛后的原料按照等量递增混合后，加入辅料，制粒，干燥，按照常规工艺制备成胶囊剂型或片剂。
实施例5
一种具有全面抗氧化功能的保健食品，按质量百分比用下述原料制成：
葡萄籽提取物95mg，虎杖提取物0.25mg，姜黄提取物0.25mg，绿茶提取物0.25mg，芦荟凝胶冻干粉200:1 0.25mg，柑橘生物类黄酮2mg，微晶纤维素125mg，硬脂酸镁45mg，二氧化硅40mg。将所述原料过80目筛，将过筛后的原料按照等量递增混合后，加入辅料，制粒，干燥，按照常规工艺制备成胶囊剂型或片剂。
实施例6
一种具有全面抗氧化功能的保健食品，按质量百分比用下述原料制成：
葡萄籽提取物30mg，虎杖提取物0.15mg，姜黄提取物0.15mg， 绿茶提取物60mg，芦荟凝胶冻干粉200:1 47.7mg，柑橘生物类黄酮87mg，微晶纤维素30mg，硬脂酸镁30mg，二氧化硅15mg。将所述原料过80目筛，将过筛后的原料按照等量递增混合后，加入辅料，制粒，干燥，按照常规工艺制备成胶囊剂型或片剂。
本发明中的辅料与现有技术中制备胶囊或片剂类似，可以根据具体需求采用其他用于胶囊、片剂所需的稀释剂、润滑剂、助流剂等，在此不做具体细化。
本发明还揭示了一种以ORAC 5.0分析技术及CAA细胞抗氧化活性评价方法为依托，对所发明全面抗氧化保健食品实例1～6进行了评价和测试。验证了本发明一种全面抗氧化功能的保健食品的作用，能够清除过氧自由基(ROO·)、羟自由基(·OH)、超氧阴离子(O 2－·)、单线态氧( 1O 2)、过氧亚硝酸基(ONOO －)。
ORAC 5.0分析技术法原理为：将检测剂(荧光探针)和活性氧/活性氮(ROS/RNS)产生剂(底物)加入样品(抗氧化剂)中，ROS/RNS攻击荧光探针，产生荧光变化(荧光强度增强或减弱)，一旦受到抗氧化剂作用，体系中的抗氧化剂竞争性抑制检测剂与底物反应，从而抑制荧光变化。该方法以Trolox作为阳性对照，结合荧光强度的变化评价样品(抗氧化剂)的ROS/RNS清除能力。
细胞抗氧化活性(cellular antioxidant activity，CAA)评价方法，模拟生物体内部环境，检测待测样品氧自由基吸收能力的方法。其原理为：荧光探针DCFH-DA可以自由穿过细胞膜，进入细胞内，并被细胞内的酯酶水解生成DCFH，后者不能自由穿过细胞膜，因此就被装载入细胞内。在细胞内，活性氧自由基可以把没有荧光的DCFH氧化成有荧光的DCF。该方法以Quercetin作为阳性对照，通过检测DCF的荧光强度变化评价细胞内活性氧自由基的水平。
通过该两项技术对所发明保健食品实例1～6的自由基清除能力进行了评价和测试，ORAC值(过氧自由基清除能力)在5000～6900μmolTE/g，HORAC值(羟基自由基清除能力)在9000～16500μmolTE/g，SORAC值(超氧阴离子清除能力)在3600～5200μmolTE/g，SOAC值(单线态氧清除能力)在1200～6520μmolTE/g，NORAC值(过氧亚硝基清除能力)在260～440μmolTE/g，细胞抗氧化活性CAA值在155～240μmol QE/g，成功表明具有很明显全面的抗氧化能力。
当然本发明尚有多种具体的实施方式，在此就不一一列举。凡采用等同替换或者等效变换而形成的所有技术方案，均落在本发明要求保护的范围之内。

Claims (10)

  1. 一种全面抗氧化保健食品，其特征在于，所述食品主要由以下成分按质量百分比组成：葡萄籽提取物10％～60％，虎杖提取物0.05％～30％，姜黄提取物0.05％～30％，绿茶提取物0.05％～30％，芦荟凝胶冻干粉200:1 0.05％～30％，柑橘生物类黄酮0.05％～30％，其中所述葡萄籽提取物中多酚含量≥85％，所述姜黄提取物中姜黄素含量≥90％，所述虎杖提取物中白藜芦醇含量≥45％，所述绿茶提取物中茶多酚含量≥90％。
  2. 如权利要求1所述的一种全面抗氧化保健食品，其特征在于：所述食品中还包括微晶纤维素10％～50％，硬脂酸镁5％～40％，二氧化硅2％～35％。
  3. 如权利要求2所述的一种全面抗氧化保健食品，其特征在于：所述食品主要由以下成分按质量百分比组成：葡萄籽提取物20％～60％，虎杖提取物0.05％～10％，姜黄提取物0.05％～20％，绿茶提取物0.05％～20％，芦荟凝胶冻干粉200:1 0.05％～20％，柑橘生物类黄酮0.05％～20％。
  4. 如权利要求3所述的一种全面抗氧化保健食品，其特征在于：所述食品主要由以下成分按质量百分比组成：葡萄籽提取物20％～50％，虎杖提取物0.05％～5％，姜黄提取物0.05％～10％，绿茶提取物0.05％～10％，芦荟凝胶冻干粉200:1 0.05％～10％，柑橘生物类黄酮0.05％～10％。
  5. 如权利要求4所述的一种全面抗氧化保健食品，其特征在于：所述食品主要由以下成分按质量百分比组成：葡萄籽提取物30％～40％，虎杖提取物0.05％～3％，姜黄提取物0.05％～5％，绿茶提取物0.05％～5％，芦荟凝胶冻干粉200:1 0.05％～5％，柑橘生物类黄酮0.05％～5％。
  6. 如权利要求2所述的一种全面抗氧化保健食品，其特征在于：所述食品中还包括微晶纤维素20％～40％，硬脂酸镁10％～20％，二氧化硅5％～20％。
  7. 如权利要求6所述的一种全面抗氧化保健食品，其特征在于：所述食品中还包括微晶纤维素30％～40％，硬脂酸镁12％～18％，二氧化硅10％～18％。
  8. 如权利要求7所述的一种全面抗氧化保健食品，其特征在于：所述食品中还包括微晶纤维素35％～40％，硬脂酸镁13％～17％，二氧化硅10％～17％。
  9. 如权利要求1所述的一种全面抗氧化保健食品，其特征在于：所述抗氧化包括清除过氧自由基、羟自由基、超氧阴离子、单线态氧、过氧亚硝酸基。
  10. 如权利要求1所述的一种全面抗氧化保健食品的制备方法，其特征在于：包括如下步骤，
    S1、原料准备，按质量百分比称取原料：葡萄籽提取物10％～60％，虎杖提取物0.05％～30％，姜黄提取物0.05％～30％，绿茶提取物0.05％～30％，芦荟凝胶冻干粉200:1 0.05％～30％，柑橘生物类黄酮0.05％～30％，其中所述葡萄籽提取物中多酚含量≥85％，所述姜黄提取物中姜黄素含量≥90％，所述虎杖提取物中白藜芦醇含量≥45％，所述绿茶提取物中茶多酚含量≥90％；
    S2、筛选，将准备好的原料并经过70-90目筛进行筛选，选出粒度均匀的原料待用；
    S3、混合，将过筛后的原料按照等量递增的方法混合均匀；
    S4、辅料添加，在S3中加入辅料，进行再次混合，制粒，干燥，制备成所需的胶囊剂型或片剂所述辅料为微晶纤维素10％～50％，硬脂酸镁5％～40％，二氧化硅2％～35％。
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