WO2019016492A1 - Composition comprenant une concentration élevée en lipopeptides ituriniques - Google Patents
Composition comprenant une concentration élevée en lipopeptides ituriniques Download PDFInfo
- Publication number
- WO2019016492A1 WO2019016492A1 PCT/FR2018/051883 FR2018051883W WO2019016492A1 WO 2019016492 A1 WO2019016492 A1 WO 2019016492A1 FR 2018051883 W FR2018051883 W FR 2018051883W WO 2019016492 A1 WO2019016492 A1 WO 2019016492A1
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- surfactants
- composition
- concentration
- lipopeptides
- iturinic
- Prior art date
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Classifications
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- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/20—Bacteria; Substances produced thereby or obtained therefrom
- A01N63/22—Bacillus
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- A—HUMAN NECESSITIES
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- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/50—Isolated enzymes; Isolated proteins
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
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- A23L33/115—Fatty acids or derivatives thereof; Fats or oils
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/135—Bacteria or derivatives thereof, e.g. probiotics
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
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- A23L33/195—Proteins from microorganisms
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- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/66—Microorganisms or materials therefrom
- A61K35/74—Bacteria
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- A61K35/742—Spore-forming bacteria, e.g. Bacillus coagulans, Bacillus subtilis, clostridium or Lactobacillus sporogenes
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- A61K38/04—Peptides having up to 20 amino acids in a fully defined sequence; Derivatives thereof
- A61K38/12—Cyclic peptides, e.g. bacitracins; Polymyxins; Gramicidins S, C; Tyrocidins A, B or C
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- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/64—Proteins; Peptides; Derivatives or degradation products thereof
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- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/10—General cosmetic use
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
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- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/0087—Galenical forms not covered by A61K9/02 - A61K9/7023
- A61K9/0095—Drinks; Beverages; Syrups; Compositions for reconstitution thereof, e.g. powders or tablets to be dispersed in a glass of water; Veterinary drenches
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- A61K9/14—Particulate form, e.g. powders, Processes for size reducing of pure drugs or the resulting products, Pure drug nanoparticles
- A61K9/19—Particulate form, e.g. powders, Processes for size reducing of pure drugs or the resulting products, Pure drug nanoparticles lyophilised, i.e. freeze-dried, solutions or dispersions
Definitions
- the invention relates to compositions having a high concentration of iturinic lipopeptides.
- the compositions according to the invention have a concentration of iturinic lipopeptides of between 5 and 200 g / l, and are stable and homogeneous thanks to the presence of surfactants and / or hydrotropic molecules.
- Iturinic lipopeptides are molecules produced by different strains of Bacillus sp. and more particularly the strains of Bacillus subtilis, Bacillus amyloliquefaciens, Bacillus megaterium, Bacillus thermoamylovorans, Bacillus thermocloacae, Bacillus firmus, Bacillus mojavensis, Bacillus velenzensis, Bacillus valissmortis.
- This family of molecules includes iturin A, AL and C, mojavensin, mycosubtillin and bacillomycins A, B, C, D, F, L and Le. These components are known mainly for their antifungal power but they also have antibacterial properties. These properties come from their amphiphilic nature which allows them to interact with different membrane constituents.
- the iturinic molecules can be produced in solution in the fermentation broth by the various strains mentioned above, then they can be recovered according to methods known to those skilled in the art in order to extract, concentrate and purify them. from a culture supernatant. Nevertheless, their behavior in solution is dependent on their concentration. Indeed, it is known that above the critical micellar concentration (situated between 10 and 20 mg / L), iturinic compounds like the other lipopeptide-type molecules form more or less complex micelles.
- these micelles will evolve to form increasingly complex structures when increasing their concentration, from an average size of 10 nm to concentrations below 500 mg / L (Jauregi et al., 2013), to vesicular structures with an average diameter of 150 nm above 1 g / L (Grau et al., 2001), then to lamellar bilayer-type superstructures at a concentration of 10 g / L (Hamley et al., 2013). As revealed by this work on mycosubtiline (Hamley et al., 2013) or iturin A (Grau et al., 2001), these superstructures are not found in other lipopeptide molecules of the surfactin family, for example. Moreover increasing the concentration of iturinic compounds both to increase their interaction with the proteins contained in the culture medium and thus to increase the size of the structures leading to make them insoluble (Jauregi et al., 2013).
- the prior art has few compositions having a concentration of iturinic heptopeptides greater than 5 g / L and even less having a concentration greater than 20 g / L, mainly because it is known to those skilled in the art that iturinic itepopeptides tend to form miceiies at high concentration, making the compositions unstable due to the miceiies of iturine preventing their good solubility.
- JP2003 128512 SHOWA DENKO
- Cosmetic compositions including surfactants, in which are added iurine and surfactin for their antimicrobial properties, but stability and The homogeneity ofiturine in these compositions is not mentioned, since these are not the main compound of the various compositions.
- the inventors have shown that it is possible to prepare a stable and homogeneous composition in which the concentration of iturinic lipopeptides is greater than 5 g / L by the addition of molecules having surfactant properties. Indeed, the inventors have demonstrated that the surfactants make it possible to improve the solubilization of iturinic lipopeptides beyond 5 g / l, in particular at 10 g / l and 20 g / l and up to 50, 100, even 200 g / L without appearance of precipitate or gel.
- the present invention finds particular applications in the production of concentrated solutions of biopesticide antifungal, antibacterial or biosurfactant molecules for the phytosanitary industry, but also in the fields of the food, cosmetic, pharmaceutical and petroleum industries.
- the general concept of the invention relates to a stable and homogeneous liquid composition
- a lipopeptide mixture in which the concentration of iturinic lipopeptides is greater than 5 g / l.
- the lipopeptides are associated with molecules having surface-active properties, by virtue of which the concentrated composition of iturinic lipopeptides remains stable and homogeneous.
- a first subject of the invention relates to a stable and homogeneous composition
- a stable and homogeneous composition comprising a concentration of iturinic lipopeptides greater than 20 g / l and one or more surfactants chosen from the families of anionic surfactants, nonionic surfactants or oils. .
- stable and homogeneous composition is meant a composition which does not comprise a precipitate and which remains homogeneous at temperatures of between 4 ° C. and 40 ° C.
- the absence of caking is also an important criterion, that is to say that the solution is liquid and not in gelled form.
- liquid is understood in the sense of the invention as a fluid composition that is to say a composition whose viscosity (the ratio between the shear stress and the shear rate) is low.
- the homogeneous term is understood as a mixture of which the various constituents can not be distinguished with the naked eye after stirring. Homogeneity can be assessed by the uniformity of the concentrations at any point in the mixture and the absence of structures or micro-structures (mass gain or precipitate) visible to the eye. Such homogeneity of the mixture is advantageous because it allows uniform concentrations of iturinic lipopeptides, which can then be diluted in aqueous solution.
- a stable and homogeneous mixture is a mixture having a uniform concentration which remains constant over time.
- the composition according to the invention comprises between 20 and 200 g / L of iturinic lipopeptides. It may comprise, for example, 20, 25, 30, 40, 50, 60, 70, 80, 90, 100 or 200 g / L of iturinic lipopeptides.
- the concentration of iturinic lipopeptides of the composition is between 20 and 150 g / l, or between 20 and 100 g / l. Even more preferably, the concentration of iturinic lipopeptides of the composition is greater than 50 g / l, that is to say for example between 50 and 150 g / l, or between 50 and 100 g / l or between 50 and 80 g / L.
- iturin family molecules is meant iturin A, AL and C, mojavensin, mycosubtilin and bacillomycins A, B, C, D, F, L and Le.
- compositions of the present invention include molecules having surfactant properties.
- molecules having surfactant properties also called “surfactants” or simply “surfactants” is meant especially amphiphilic molecules, surfactants, lipopeptides, such as, for example, surfactin, fengycin (or plipastatin), chemical surfactants and biological surfactants, such as rhamnolipids, polysaccharides, etc.
- surfactants also called “surfactants” or simply “surfactants” is meant especially amphiphilic molecules, surfactants, lipopeptides, such as, for example, surfactin, fengycin (or plipastatin), chemical surfactants and biological surfactants, such as rhamnolipids, polysaccharides, etc.
- surfactants also called “surfactants” or simply “surfactants” is meant especially amphiphilic molecules, surfactants, lipopeptides, such as, for example, surfactin, fengycin (or plipastatin), chemical surfactants and biological surfactants, such
- surfactants mention may be made of heparin, hyaluronic acid, dextran, amylose, chitosan, anionic surfactants derived from amino acids, nonionic surfactants derived from poly-glycosides, surfactants, hydrotropic active agents, lipopeptides such as isomers of surfactin and / or the isomers of fengycin (or plipastatin), rhamnolipids and vegetable oils.
- the invention relates to anionic surfactants, nonionic surfactants and oils.
- the surfactants of the family of anionic surfactants are chosen from: surfactins, fengycins, or amino acid derivatives.
- Surfactin means any molecule of the "family of surfactins", namely surfactins A, B, C, lichenysine and pumilacidine.
- fengycin any molecule of the "fengycin family", is meant fengycins A and B, plipastatin A and B and agrastatin.
- the surfactants of the family of nonionic surfactants are chosen from: fatty alcohol axalkylate, pentylene glycol and its derivatives, hydrotropic molecules of alkylpolyglycoside type (alkypolyglycoside and alkylethoxypolyglycoside, polyglycoside texturing agent-type molecules (xanthan gum , gum arabic, gum tragacanth, guar gum, locust bean gum, tamarind gum, pectin, gellan gum, carrageenates, agar-agar, alginates.)
- the surfactants of the family of oils are chosen from: oils and extracts of modified oils (acifidies, methylated, esterified), especially derived from: almond, peanut, argan, avocado, rapeseed, lorenzo, neem, hazelnut, cashew nut, macadamia nut, olive, pistachio, rice, oleic sunflower, camelina, flax, borage, safflower, hemp, cotton, wheat germ, corn, nut, flaxseed, evening primrose, barley, pumpkin seeds, grape seeds, peas, sesame , soya, sunflower.
- the iturinic lipopeptides will be micro-emulsified in these oily compounds or fats in order to obtain the surfactant effect of the oils and extracts of modified oils.
- compositions of the invention may further contain adjuvants such as lipid sources, salts and solvents. These adjuvants can participate in the solubilization without being surfactants.
- adjuvants such as lipid sources, salts and solvents.
- solvents mention may be made of ethanol, methanol, acetonitrile, dimethyl sulfoxide, butanol, pentanol and acetone.
- the invention relates to a composition
- a composition comprising between 5 and 50 g / l of iturinic lipopeptides and between 1 and 40 g / l of surfactin.
- iturinic lipopeptides are mycosubtilines.
- the amount of iturinic lipopeptides is preferably between 10 g / l and 50 g / l, preferably between 20 and 50 g / l, more preferably between 30 and 50 g / l.
- the invention relates to a composition comprising between 5 and 100 g / l of iturinic lipopeptides, between 1 and 40 g / l of surfactin and between 1 and 100 g / l of fengycin (or plipastatin).
- the amount of iturinic lipopeptides is preferably between 10 and 80 g / l, preferably between 20 and 80 g / l, most preferably between 30 and 80 g / l.
- the invention relates to a composition
- a composition comprising between 5 and 100 g / l of iturinic lipopeptides, between 1 and 40 g / l of surfactin and between 1 and 30 g / l of chemical surfactants.
- the amount of iturinic lipopeptides is preferably between 10 and 80 g / l, preferably between 20 and 80 g / l, preferably between 30 and 80 g / l.
- the chemical surfactants may be nonionic surfactants of the family of polyglycosides or anionic surfactants derived from amino acids.
- the chemical surfactants consist of a mixture of anionic surfactants and nonionic surfactants.
- the invention relates to a composition
- a composition comprising between 5 and 100 g / l of iturinic lipopeptides, between 1 and 40 g / l of surfactin, between 1 and 100 g / l of fengycin (or plipastatin) and between 1 and 30 g / l of chemical surfactants.
- the amount of iturinic lipopeptides is preferably between 10 and 80 g / l, preferably between 20 and 80 g / l, preferably between 30 and 80 g / l.
- the invention relates to a composition
- a composition comprising between 5 and 100 g / L of micro-emulsified iturinic lipopeptides in oily compounds or fatty substances, for example vegetable oil.
- the oil concentration is between 1% and 100%, preferably between 50% and 100%, particularly preferably between 80% and 100%.
- corn oil but also modified almond, peanut, argan, avocado, rapeseed, lorenzo, neem, hazelnut, and cashew nut oils and extracts, macadamia nut, olive, pistachio, rice, oleic sunflower, camelina, flax, borage, safflower, hemp, cotton, wheat germ, corn, nut, flaxseed, evening primrose, barley, pumpkin seeds, grape seeds, peas, sesame , soy, and sunflower.
- the oils used are derived from peanut, olive, oleic sunflower, flax, corn, nuts, soya and sunflower. Such oils present the advantage of being available in large quantities and having a low cost. Even more preferably, the oil used is derived from corn peanuts or sunflower.
- such a composition comprises between 10 and 80 g / l, preferably between 20 and 80 g / l of mycosubtiline, preferably between 30 and 80 g / l and very preferably between 50 and 80 g / l. g / L of mycosubtilin.
- composition according to the invention comprises:
- the composition comprises about 3% of lipopeptides (1.25% of mycosubtilin and 1.85% of surfactin), 1.5% of a non-surfactant. ionic derivative derived from poly-glycosides and 1.5% of anionic surfactant derived from amino acids.
- the composition comprises about 5% lipopeptides (2.45% mycosubtiline and 2.65% surfactin) and 3-8% fengycin (or plipastatin).
- the composition comprises between 30 and 80 g / l of mycosubtiline and 30 to 80 g / l of fengycin (or plipastatin).
- the composition comprises a concentration of iturinic lipopeptides is between 20 and 100g / l and the surfactants are a vegetable oil extract, derivatives of polyglycosides and surfactin.
- the composition comprises a concentration of iturinic lipopeptides is between 20 and 100g / l and the surfactants are an extract of methylated vegetable seed oil and polyglycerol ester of coconut oil (Synergen OS TM) .
- the composition comprises a concentration of iturinic lipopeptides of between 20 and 100 g / l and the surfactants are a mixture of alkylpolyglucoside (Simulsol TM) amino acid derivative (Proteol TM) and surfactin.
- the surfactants are a mixture of alkylpolyglucoside (Simulsol TM) amino acid derivative (Proteol TM) and surfactin.
- composition comprises a concentration of iturinic lipopeptides is between 20 and 100g / l and the surfactants are a mixture of fatty alcohol oxalkylate (Emulsogen TM) and surfactin.
- surfactants are a mixture of fatty alcohol oxalkylate (Emulsogen TM) and surfactin.
- composition comprises a concentration of iturinic lipopeptides is between 20 and 100g / l and the surfactants are a mixture of vegetable oil, xanthan gum and surfactin.
- compositions according to the invention may be ready for use or in concentrated form; concentrated solutions can be diluted before use.
- a second object according to the invention relates to a dehydrated composition comprising a lipopeptide mixture in which the concentration of iturinic lipopeptides is greater than 0.5%. In a preferred embodiment, the concentration of iturinic lipopeptides is greater than 2%.
- compositions are in the form of powder or lyophilizate and are "ready to use”; indeed, it is sufficient to rehydrate in 100 ml of solution to obtain a composition whose concentration of iturinic lipopeptides is at least 5 g / l. In a preferred embodiment, such compositions have a concentration of iturinic lipopeptides of at least 20 g / L.
- the concentrations by dry weight of the various components of these dehydrated solutions make it possible to obtain liquid compositions whose concentration of iturinic lipopeptides is greater than 5 g / l which are stable and homogeneous in accordance with the invention. In a preferred embodiment, the concentration of iturinic lipopeptides is greater than 20 g / l.
- a third subject of the invention relates to a method for preparing a composition comprising a lipopeptide mixture in which the concentration of iturinic lipopeptides is greater than 5 g / l. In a preferred embodiment, the concentration of iturinic lipopeptides in the mixture is 20 g / l.
- the iturinic lipopeptides of the composition can be obtained by fermentation of a strain of Bacillus sp. and harvested and concentrated from the culture supernatant.
- the compositions according to the invention can be prepared using lipopeptide preparations commercially available in powder form. These starting powders contain a variable amount of iturinic lipopeptides which may range from 10 to more than 80%, for example 15% or 75%. These powders may contain a mixture of mycosubtilin and surfactin in a ratio of 30/70 to 70/30, or even 95/5, for example 35/65, 40/60, 45/55 or 50/50. .
- At least one surfactant must be added to a solution containing a high content of iturinic lipopeptides.
- the process for preparing such compositions may include an additional dehydration step so as to provide a ready-to-use powder form composition, the characteristics of which are in accordance with the compositions of the present invention when rehydrated.
- the method makes it possible to prepare liquid or solid solutions (in the form of powder or lyophilisate). Such concentrated solutions are particularly suitable for storage and distribution. They are to be diluted before use. As a result, a composition according to the invention can be obtained by dissolving a powder allowing the reconstitution of a liquid composition as defined above.
- compositions according to the invention find their applications in the field of agri-food, phytosanitary and cosmetic as well as in the medical and pharmaceutical field.
- the lipopeptides are obtained from an aerobic fermentation process of a Bacillus strain derived from the strain Bacillus subtilis ATCC 6633 for the production of lipopeptides iturinic and strains derived from the Bacillus subtilis 168 strain for the production of surfactin and / or fengycin (or plipastatin).
- the culture is carried out in a medium containing a source of carbon (glucose, sucrose, etc.), a source of nitrogen (ammonium sulphate, peptone, free amino acids, yeast extract, etc.) and trace elements and salts with stirring at 30 ° C.
- the pH is maintained at a value of 7.
- the crop is harvested after 48 to 72 hours. It is then centrifuged or filtered to remove the cells.
- the culture supernatant is then concentrated.
- compositions with a high content of iturinic lipopeptides from culture supernatants
- the culture supernatants can be concentrated.
- concentration of the culture supernatant can be obtained by any method known to those skilled in the art, in particular:
- the supernatant is concentrated by evaporation under vacuum. For example 20L are introduced into a rotavapor and concentrated to 1 to 2L.
- the percentage of lipopeptides obtained at the end of these two examples of preparation is of the order of 0.5 to 20% (weight / volume).
- composition 1 which contains only iturinic lipopeptides.
- the surfactants added in the compositions 2 to 13 are:
- composition 2 10% fengycins (composition 2)
- soybean lecithin (4%) and poly-glycoside derivatives (0.1%) (Elvis TM / Xanthan Gum) (composition 4)
- composition 5 of methyl vegetable seed oil extract and polyglycerol ester derived from coconut oil (0.2%) (Synergen OS TM) (composition 5)
- nonionic surfactant of the fatty alcohol oxalkylate (0.2%) type (Emulsogen TM) (composition 6)
- nonionic surfactant pentylene glycol 50%) (composition 7)
- composition 10 surfactines at 4% and fengycins at 10% (composition 10)
- composition 11 vegetable oil extract (30%) and derivatives of poly-glycosides (0.1%) and surfactin (4%) (corn oil / Xanthan Gum / Surfactin) (composition 11)
- Figure 1 shows the solubility of iturinic lipopeptides (in this case mycosubtiline) in aqueous solution at room temperature as a function of their concentration
- FIG. 2 shows the analysis of the solubility of various compositions comprising an iturinic lipopeptide (in this case mycosubtilin) and various compounds making it possible to obtain high and soluble concentrations of iturinic lipopeptide after 15 days of storage at ambient temperature. The measurement is made on the supernatant after a centrifugation step to eliminate the insolubles
- nonionic compounds such as penthylene glycol and alkylethoxyglycoside also make it possible to obtain a solubility of greater than 30 g / l.
- the composition allowing a maximum solubility of the iturinic compound (112 g / L) is that containing an anionic lipopeptide (surfactin) and a nonionic surfactant (Emulsogen TM).
- compositions with a high content of iturinic lipopeptides can be prepared from lipopeptide powder, as illustrated in the following two examples of compositions:
- Composition 1 A composition comprising approximately 3.1% (w / v) of lipopeptides (ie 1.25% mycosubtiline and 1.85% surfactin) was obtained by resuspending a lipopeptide powder comprising a mycosubtilin / surfactin mixture in proportions of 40%. 60 and a purity of about 15% in aqueous phase (pH between 7.5 and 8.5).
- Composition 2 A composition comprising approximately 5.1% (w / v) of lipopeptides (ie 2.45% mycosubtiline and 2.65% surfactin) was obtained by resuspending a lipopeptide powder comprising a mycosubtilin / surfactin mixture in proportions 45/55 and a purity of about 75% in aqueous phase (pH between 7.5 and 8.5).
- lipopeptides ie 2.45% mycosubtiline and 2.65% surfactin
- compositions with a high content of iturinic lipopeptides comprising surfactants Preparation of compositions with a high content of iturinic lipopeptides comprising surfactants.
- compositions 1 and 2 were added to compositions 1 and 2 described above so as to evaluate the effect of such molecules on the properties of the compositions.
- composition 2A 3 to 8% (w / v) fengycin (or plipastatin) was added to composition 2.
- compositions with and without surfactants has been studied at ambient temperature, here at about 21 ° C.
- composition 1 The addition of the two surfactants made it possible to obtain a homogeneous composition (composition 1A) and to avoid any phenomenon of precipitation or sedimentation.
- composition 1A containing surfactants has a cloudy but homogeneous appearance.
- Composition 2 The addition of fengycin (or plipastatin) made it possible to obtain a homogeneous composition and to avoid any phenomenon of setting in mass (which takes place in the absence of fengycin or plipastatin).
- Composition 2A with fengycin (or plipastatin) has a clear and homogeneous appearance.
- Concentrated lipopeptide solutions (Compositions 1, 2 and 1A, 2A) were placed at 4 ° C for a period of 1 to 30 days. Only solutions supplemented with surfactants or fengycin (or plipastatin) (namely 1A and 2A) remained homogeneous and no deposit was observed. Without these additions, concentrated lipopeptide solutions are likely to set in bulk or to have a large insoluble deposit.
- compositions described above (1 and 2) and those containing adjuvants (1A and 2A) were stored for 15 days at 21 ° C and for 15 days at 54 ° C (to simulate accelerated aging).
- the preservation of compositions at elevated temperature (54 ° C.) is a method known to those skilled in the art, which makes it possible to mimic accelerated aging of the compositions by increasing the Brownian motion, accelerating the destabilization of the compositions.
- FIG. 3 shows the analysis of the minimum viscosity of the various concentrated iturin compositions without or with additions of surfactants and other adjuvants (A), after storage at 21 ° C. or at 54 ° C.
- FIG. 4 presents the analysis of the maximum viscosity shear stress of the various lipopeptide compositions concentrated without or with additions of surfactants and other adjuvants (A), after storage at 21 ° C. or at 54 ° C.
- composition 1A makes it possible to solubilize the iturinic compounds avoiding the formation of precipitate and / or gel and this is characterized by a significant decrease in the minimum viscosity and the shear stress at maximum viscosity, whether after storage at 21 ° C. or even more importantly when aging.
- Compositions 1 and 1A are related to non-Newtonian fluids of the Casson type
- composition 2A makes it possible to solubilize the iturinic compounds avoiding the formation of precipitate and / or gel at the end of a long storage period and this is characterized by a significant decrease in the minimum viscosity and the shear stress at the viscosity. maximum after storage at 54 ° C.
- Compositions 2 and 2A are related to non-Newtonian fluids of rheofluidifying type initially, but the unformulated composition 2 becomes very thick during aging.
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Priority Applications (7)
Application Number | Priority Date | Filing Date | Title |
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JP2020502168A JP2020527348A (ja) | 2017-07-21 | 2018-07-23 | 高濃度のイツリン系リポペプチドを含む組成物 |
CA3069760A CA3069760A1 (fr) | 2017-07-21 | 2018-07-23 | Composition comprenant une concentration elevee en lipopeptides ituriniques |
EP18758926.2A EP3654945A1 (fr) | 2017-07-21 | 2018-07-23 | Composition comprenant une concentration élevée en lipopeptides ituriniques |
BR112020001291-2A BR112020001291A2 (pt) | 2017-07-21 | 2018-07-23 | composição compreendendo uma alta concentração de lipopeptídeos iturínicos |
US16/632,061 US20200147169A1 (en) | 2017-07-21 | 2018-07-23 | Composition comprising a high concentration of iturin lipopeptides |
CN201880048741.3A CN110944626A (zh) | 2017-07-21 | 2018-07-23 | 包含高浓度伊枯草菌素脂肽的组合物 |
AU2018303287A AU2018303287A1 (en) | 2017-07-21 | 2018-07-23 | Composition comprising a high concentration of iturin lipopeptides |
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FR17/56909 | 2017-07-21 | ||
FR1756909A FR3069155B1 (fr) | 2017-07-21 | 2017-07-21 | Composition comprenant une concentration elevee en lipopeptides ituriniques |
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EP (1) | EP3654945A1 (fr) |
JP (1) | JP2020527348A (fr) |
CN (1) | CN110944626A (fr) |
AU (1) | AU2018303287A1 (fr) |
BR (1) | BR112020001291A2 (fr) |
CA (1) | CA3069760A1 (fr) |
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Citations (3)
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JP2003128512A (ja) | 2001-10-18 | 2003-05-08 | Showa Denko Kk | 化粧料用抗菌性組成物 |
CN101948682A (zh) * | 2010-08-20 | 2011-01-19 | 中国石油天然气股份有限公司 | 一种反相堆积的油井防偏磨、缓蚀清防垢剂 |
US20160183537A1 (en) | 2014-12-29 | 2016-06-30 | Fmc Corporation | Bacillus amyloliquefaciens rti472 compositions and methods of use for benefiting plant growth and treating plant disease |
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JPS59212416A (ja) * | 1983-05-19 | 1984-12-01 | Ajinomoto Co Inc | 農業用殺菌剤 |
WO1998050422A1 (fr) * | 1997-05-09 | 1998-11-12 | Agraquest, Inc. | Nouvelle souche de bacillus destinee a lutter contre les maladies vegetales et la larve nuisible aux racines du mais |
JP2003113040A (ja) * | 2001-10-04 | 2003-04-18 | Showa Denko Kk | 抗菌性化粧料組成物 |
JP2005053867A (ja) * | 2003-08-07 | 2005-03-03 | Showa Denko Kk | フケ抑制組成物 |
WO2012162412A2 (fr) * | 2011-05-24 | 2012-11-29 | Agraquest, Inc., A Delaware Corporation | Combinaisons synergiques de fongicides de type polyène et de peptides non ribosomaux et procédés d'utilisation associés |
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2017
- 2017-07-21 FR FR1756909A patent/FR3069155B1/fr active Active
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2018
- 2018-07-23 CA CA3069760A patent/CA3069760A1/fr active Pending
- 2018-07-23 CN CN201880048741.3A patent/CN110944626A/zh active Pending
- 2018-07-23 JP JP2020502168A patent/JP2020527348A/ja active Pending
- 2018-07-23 AU AU2018303287A patent/AU2018303287A1/en active Pending
- 2018-07-23 WO PCT/FR2018/051883 patent/WO2019016492A1/fr unknown
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- 2018-07-23 EP EP18758926.2A patent/EP3654945A1/fr active Pending
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Publication number | Priority date | Publication date | Assignee | Title |
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JP2003128512A (ja) | 2001-10-18 | 2003-05-08 | Showa Denko Kk | 化粧料用抗菌性組成物 |
CN101948682A (zh) * | 2010-08-20 | 2011-01-19 | 中国石油天然气股份有限公司 | 一种反相堆积的油井防偏磨、缓蚀清防垢剂 |
US20160183537A1 (en) | 2014-12-29 | 2016-06-30 | Fmc Corporation | Bacillus amyloliquefaciens rti472 compositions and methods of use for benefiting plant growth and treating plant disease |
Non-Patent Citations (2)
Title |
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CHOUKRI HBID ET AL: "Influence of the production of two lipopeptides, Iturin a and Surfactin S1, on oxygen transfer duringfermentation", APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY ; PART A: ENZYME ENGINEERING AND BIOTECHNOLOGY, HUMANA PRESS INC, NEW YORK, vol. 57 - 58, no. 1, 1 March 1996 (1996-03-01), pages 571 - 579, XP035175765, ISSN: 1559-0291, DOI: 10.1007/BF02941737 * |
See also references of EP3654945A1 |
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US20200147169A1 (en) | 2020-05-14 |
FR3069155A1 (fr) | 2019-01-25 |
BR112020001291A2 (pt) | 2020-07-21 |
JP2020527348A (ja) | 2020-09-10 |
EP3654945A1 (fr) | 2020-05-27 |
CN110944626A (zh) | 2020-03-31 |
CA3069760A1 (fr) | 2019-01-24 |
AU2018303287A1 (en) | 2020-02-13 |
FR3069155B1 (fr) | 2020-07-10 |
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