WO2019014958A1

WO2019014958A1 - Biological sample collecting and processing bottle, and method of use therefor

Info

Publication number: WO2019014958A1
Application number: PCT/CN2017/094570
Authority: WO
Inventors: 尤其敏; 胡林; 杨帆; 赵芯; 金荣愉; 何晟; 陈思思
Original assignee: 杭州优思达生物技术有限公司
Priority date: 2017-07-18
Filing date: 2017-07-26
Publication date: 2019-01-24
Also published as: CN107202725A; CN107202725B

Abstract

Disclosed is a biological sample collecting and processing bottle, comprising: a bottle body (1) for storing an in-built sample processing liquid, wherein an opening (2) is provided in the bottom of a bottleneck, and a positioning rib (3) is arranged on a lower side wall of the bottle body; a retractable pipe assembly (4) arranged on an upper part of the bottle body and fixedly connected to the bottle body, wherein the bottleneck (5) is arranged on an upper part of the pipe assembly, a flange (6) is fixed on an inner side wall of the bottleneck, an inner cap (7) is arranged in the bottleneck, the inner cap is in contact with the flange in a slidable manner, and the bottleneck zone above the inner cap is a sample storage cavity (8); an ejector head (9) with an upper part being abutted against the inner cap, the ejector head being fixed to the positioning rib of the bottle body; and an outer cap (10) arranged at the bottleneck and used for sealing the bottleneck. The inner cap, the ejector head and the retractable pipe assembly are used in the processing bottle, effectively reducing the probability of an experimenter being infected by pathogenic microorganisms in the sample and the probability of sample contamination. The experimenter does not need to directly face the experimental sample so that working experience is improved. The experiment operation is simplified, the processed sample can be directly quantitatively transferred, and the pretreatment and transferring processes of the sample do not require extra experiment instruments.

Description

Biological sample collection and treatment bottle and method of use thereof

Technical field

The invention relates to the field of manufacturing biological sample collection and treatment bottles, in particular to a biological sample collection and treatment bottle and a using method thereof.

Background technique

Existing biological samples (such as saliva, sputum, urine, and feces) are collected in an empty bottle of appropriate size. The experimenter prepares the corresponding treatment liquid and pipetting device, manually opens the bottle, and injects the corresponding treatment liquid into the bottle, and after a certain period of time, transfers the processed sample through the pipetting device. The main drawbacks are: 1) The experimenter needs to open the collection bottle to complete the processing and transfer of the biological sample. The aerosol will form during the bottle opening and operation, and the experimenter will be infected. 2) The experimenter needs to open the collection bottle. Complete the processing and transfer of biological samples, increasing the risk of contamination of the sample; 3) When the sample is processed, the experimenter needs to open the collection cap and directly face the original biological sample, which has a poor working experience; 4) Does not contain the sample treatment solution The operator needs to prepare the pipetting device and the treatment liquid, and then add the treatment liquid to the collection bottle, which requires more steps, time and cost; 5) the operator needs to prepare the pipetting device, and the collection bottle cap is opened to transfer the processed sample. It requires more steps, time and cost; 6) When the laboratory personnel are handling the sputum sample, it is necessary to transfer the high pH treatment solution, which poses a risk of burns.

Summary of the invention

The invention aims at the above problems, and proposes a biological sample collection and processing bottle, which solves the defects that the existing biological sample collection and treatment bottle is easy to be infected, has a single function, and has a poor work experience.

The technical solution adopted by the present invention is as follows:

A biological sample collection and processing bottle comprises: a bottle body for storing a built-in sample processing liquid; an opening at the bottom of the bottle mouth; a positioning rib on a lower side wall of the bottle body; and a retractable pipe body assembly, Disposed on the upper part of the bottle body and fixedly connected to the bottle body; the bottle mouth is disposed on the upper part of the pipe body assembly and fixedly connected to the pipe body assembly; the inner side wall of the bottle mouth is fixed with a flange; the inner cover is set inside the bottle mouth; the inner cover Slidably contacting the flange; the mouth area of the upper part of the inner cover is a sample storage cavity; the head, the upper part abuts the inner cover; the bottom of the head is fixed on the positioning rib of the bottle body; the tube body assembly is used for the longitudinal direction Stretching or contracting, the tubular body is contracted such that the head lifts up the inner lid; the outer lid and the outer lid are at the mouth of the bottle, and the outer lid is used to seal the mouth of the bottle.

The invention adopts an inner cover, a head and a retractable pipe body assembly to contract the pipe body assembly, the inner head will jack up the inner cover, and the bottle body is opened; then after the collection bottle is inverted, the treatment liquid will flow out of the cavity, and the raw The sample is mixed, and then the tube assembly is forwardly contracted, so that the mixed treatment liquid is dripped through the opening of the bottom, and the biological sample can be directly collected in the closed container without the need for secondary opening, without using additional experimental instruments. The pre-treatment and transfer of the sample can be completed in a few simple steps on the collector, which can greatly reduce the risk of contamination of the experimenter and the sample, and can improve the working experience of the sample preparation and simplify the experimental operation. , saving experiment time and cost.

Optionally, a lower portion of the bottle body is provided with a wrench block, and the wrench block is located at two sides of the bottle body.

Optionally, the upper portion of the bottle body has a convex protrusion.

Optionally, the biological sample collection processing bottle further includes a safety buckle having at least one pair of longitudinally juxtaposed clamping blocks and a connecting portion connecting the pair of the clamping blocks, the top of the clamping block abutting Hold the bump and the lower part against the wrench. The safety buckle is used to effectively avoid the defect of dripping the treatment liquid by mistake in the taking process.

Optionally, the tubular body assembly adopts a tubular body in which the foldable corrugated sheets are connected in a folding and contracting direction. The tubular body connected by the foldable crepe sheet similar to the organ structure in the direction of folding and contracting has the following three functions: longitudinally telescopic ejection of the inner cover; longitudinal stretching or contraction to release the treated liquid; deformation of the range to control the extruded liquid It is quantitative.

Optionally, the plug is composed of three support blocks, and an angle between any two support blocks is 120°.

Optionally, the bottom of the support block has a convex engaging portion, the bottom of the engaging portion has a card slot, and the card slot extends laterally to a sidewall of a vertical direction of the engaging portion, and the card slot is used for a card slot. Live positioning ribs.

Optionally, the biological sample collection processing bottle further includes an outlet cover, the outlet cover outer sleeve is outside the opening, and the outlet cover is threadedly engaged with the opening.

Optionally, the upper surface of the outer cover has a transparent area.

Optionally, the upper surface of the inner cover further has an outwardly protruding annular card edge. The annular card edge is used to collect the sample.

The invention also discloses a method for using a biological sample collection and processing bottle, comprising the following steps:

a) collecting samples: the user opens the outer cover and collects the biological sample in a closed container;

b) pre-treatment of the sample: the liquid is sealed in the biological sample collection and treatment bottle; the treatment liquid is released from the closed environment by other physical or chemical means such as extrusion or pushing;

c) Observation of the effect of sample pretreatment: If necessary, the physical properties of the internal sample can be observed by the naked eye, and the user has a good user experience;

d) Transfer of the sample: open the outlet cover and output the treated liquid by extrusion or push-up; the pathogenic microorganisms in the sample are killed due to the pre-sample treatment, and the release of the treated liquid reduces the pollution environment and infection operation. Risk.

Optionally, in step b), mixing of the sample and the processing reagent can be performed without opening the processing bottle to complete the sample pretreatment.

Optionally, in step c), the sample properties and the process can be observed without opening the treatment bottle.

Optionally, the pre-processed sample can be output in the step d) without an external tool.

The invention has the beneficial effects that the invention adopts an inner cover, a plug and a retractable tubular body assembly, and the tubular body assembly is contracted, the inner head will lift the inner cover, the bottle body is opened; and then the collection bottle is inverted, the treatment liquid Will flow out of the cavity, mix with the biological sample, and then shrink the tube assembly forward, so that the mixed treatment liquid is dripped through the opening of the bottom, the pretreatment process of the entire sample is carried out in a sealed environment, (1) effective reduction experiment The probability of the person being infected by the pathogenic microorganism in the sample and the sample being contaminated; (2) the experimenter does not need to directly face the experimental sample, and the work experience is improved; (3) the experimental operation is simplified, and the sample can be directly transferred quantitatively after the treatment; (4) The pre-treatment and transfer process of the sample does not require additional laboratory equipment.

In addition, the safety buckle is used to effectively avoid the defect that the handling solution is dripped out by the handling process.

DRAWINGS

1 is a schematic front view showing the structure of a biological sample collection and treatment bottle of the present invention;

2 is a schematic cross-sectional structural view of a biological sample collection and treatment bottle before extrusion;

3 is a schematic cross-sectional structural view of a biological sample collection and treatment bottle after extrusion;

4 is a schematic structural view of a drip operation flow of a biological sample collection and treatment bottle.

The reference numerals in the figure are:

1, bottle body; 2, opening; 3, positioning ribs; 4, pipe body components; 5, bottle mouth; 6, flange; 7, inner cover; 8, sample storage cavity; 9, head; 10, outer cover; 11. Exit cover; 12, wrench block; 13, bump; 14, safety buckle; 15, clamp block; 16, connecting portion; 18, support block; 19, engaging portion; 20, card slot; Sheet; 22, transparent area; 23, annular card edge; a, storage location of the treatment liquid.

Detailed ways

The invention will now be described in detail in conjunction with the drawings.

The invention discloses a biological sample collection and processing bottle (see FIGS. 1, 2, 3, 4), comprising: a bottle body 1 for storing the built-in sample processing liquid; the bottom of the bottle mouth 5 is provided with an opening 2; The lower side wall of the bottle body 1 is provided with a positioning rib 3; a retractable tube body assembly 4 is disposed on the upper part of the bottle body 1 and fixedly connected to the bottle body 1; the bottle mouth 5 is disposed on the upper part of the tube body assembly 4 and fixes the connecting tube The body assembly 4; the inner side wall of the bottle mouth 5 is fixed with a flange 6; the inner cover 7 is disposed inside the bottle mouth 5; the inner cover 7 slidably contacts the flange 6; the mouth of the upper part of the inner cover 7 The area 5 is the sample storage chamber 8; the head 9 has an upper portion against the inner cover 7; the head 9 The bottom is fixed on the positioning rib 3 of the bottle body 1; the tube body assembly 4 is used for longitudinal stretching or contraction, the tube body is contracted to cause the head 9 to lift up the inner cover 7; the outer cover 10, the outer cover 10 is at the mouth of the bottle At 5, the outer cover 10 is used to seal the bottle opening 5.

The invention adopts an inner cover 7, a head 9 and a telescopic tube assembly 4 to contract the tube assembly 4, the inner head 8 will lift the inner cover, and the bottle body is opened; then, after the collection bottle is inverted, the treatment liquid will flow out. The cavity is mixed with the biological sample, and then the tube assembly is forwardly contracted, so that the mixed treatment liquid is dripped through the opening of the bottom, and the pretreatment process of the entire sample is performed in a sealed environment, (1) effectively reducing the experimenter's being The probability of pathogenic microbial infection and sample contamination in the sample; (2) the experimenter does not need to directly face the experimental sample, the work experience can be improved; (3) the experimental operation is simplified, and the sample can be directly transferred quantitatively after treatment; (4) the sample Pre-treatment and transfer processes without additional laboratory equipment.

The retractable pipe assembly of the present invention can also adopt a piston structure; the sealing of the treatment liquid is changed from the inner cover 7 to the bubble structure (the treatment liquid is sealed in the bubble environment) or the heat sealing film is sealed (the inner cover is changed to the sealing film) .

The lower part of the bottle body 1 is provided with a wrench block 12, and the wrench block 12 is located on both sides of the bottle body 1. The upper portion of the bottle body 1 has convex protrusions 13. The biological sample collection treatment bottle further includes a safety buckle 14 having at least one pair of longitudinally juxtaposed clamps 15 and a connection portion 16 connecting a pair of the clamp blocks 15, the clamp block 15 The top is against the bump 13 and the lower part is against the wrench 12. The safety buckle 14 is adopted to effectively avoid the defect that the handling liquid is dripped out by the handling process.

The tubular body assembly 4 is a tubular body in which the foldable corrugated sheets 21 are joined in a folding and contracting direction. The tubular body connected by the foldable crepe sheet 21 similar to the organ structure in the direction of folding and contracting has the following three functions: longitudinally stretching and ejecting the inner cover 7; longitudinally stretching or contracting to release the treated liquid; and the range-controlled extrusion of the deformation The liquid is quantitative.

The plug 9 is composed of three support blocks 18, and the angle between any two support blocks 18 is 120°. The bottom of the support block 18 has a convex engaging portion 19, and the bottom of the engaging portion 19 has a card slot 20 extending laterally to the vertical side wall of the engaging portion 19, the card slot 20 Used to clamp the positioning rib 3. The biological sample collection treatment bottle further includes an outlet cover 11 that is jacketed outside the opening 2, and the outlet cover 11 is threadedly engaged with the opening 2.

The upper surface of the outer cover 10 has a transparent area 22 for observing the inner sample. The inner surface of the inner cover 7 further has an outwardly protruding annular card edge 23. The annular card edge 23 is used to collect the sample.

In the step b), the sample and the processing reagent can be mixed without opening the treatment bottle to complete the sample pretreatment. In step c), the sample properties and the treatment process can be observed without opening the treatment bottle. The pre-processed sample can be output in the step d) without an external tool.

In the practice of the present invention, the user unscrews the outer cover to bring the biological sample into the collection bottle and tightens the bottle cap. When the experimenter operates, the safety buckle is pulled open, the wrench is fastened by hand and the cover of the collection bottle is pressed, the telescopic tube assembly is contracted, and the inner head pushes up the inner cover to open the cavity of the treatment liquid; After inverting the collection bottle, the treatment liquid will flow out of the cavity and mix with the biological sample; after being placed for a certain period of time, the experimenter can unscrew the outlet cover counterclockwise and press the collection bottle cap. At this time, the retractable tube assembly structure of the present invention Quantitative deformation will occur again, eventually extruding a standard amount of treated sample.

The above description is only a preferred embodiment of the present invention, and thus does not limit the scope of patent protection of the present invention. Any equivalent structural transformation made by using the specification and the drawings of the present invention is directly or indirectly applied to other related technical fields. The same is included in the scope of protection of the present invention.

Claims

A biological sample collection and treatment bottle, comprising:

a bottle body for storing the built-in sample processing liquid; the bottom of the bottle mouth is provided with an opening; and a sidewall of the lower part of the bottle body is provided with a positioning rib;

a retractable tubular body assembly disposed on the upper portion of the bottle body and fixedly connected to the bottle body;

a bottle mouth, disposed at an upper portion of the pipe body assembly and fixedly connecting the pipe body assembly; fixing a flange on an inner side wall of the bottle mouth;

The inner cover is disposed in the bottle mouth; the inner cover slidably contacts the flange; the bottle mouth area on the upper portion of the inner cover is a sample storage cavity;

a head, an upper part abuts against the inner cover; the bottom of the head is fixed on the positioning rib of the bottle body; the tube body assembly is used for longitudinal extension or contraction, and the tube body is contracted to cause the head to lift the inner cover;

The outer cover is at the mouth of the bottle, and the outer cover is used to seal the bottle mouth.
The biological sample collection and treatment bottle according to claim 1, wherein a lower portion of the bottle body is provided with a wrench block, and the wrench block is located at two sides of the bottle body.
The biological sample collection processing bottle according to claim 2, wherein the upper portion of the bottle body has a convex projection.
The biological sample collection processing bottle according to claim 3, further comprising a safety buckle having at least one pair of longitudinally juxtaposed clips and a connecting portion connecting the pair of the clips, The top of the clamp abuts the bump and the lower part abuts the wrench.
The biological sample collection and treatment bottle according to claim 1, wherein the tube assembly is a tube body in which the foldable crepe sheets are joined in a folding and contracting direction.
The biological sample collection processing bottle according to claim 1, wherein the plug is composed of three support blocks, and an angle between any two support blocks is 120°.
The biological sample collection and treatment bottle according to claim 6, wherein the bottom of the support block has a convex engaging portion, and the bottom of the engaging portion has a card slot, and the card slot extends laterally to the engagement. The vertical direction side wall, the card slot is used to catch the positioning rib.
The biological sample collection treatment bottle according to claim 1, further comprising an outlet cover, the outlet cover outer casing being outside the opening, the outlet cover being threadedly engaged with the opening.
The biological sample collection processing bottle according to claim 1, wherein the upper surface of the outer cover has a transparent region.
The biological sample collection and treatment bottle according to claim 1, wherein the upper surface of the inner cover further has an outwardly projecting annular card edge.
A method of using a biological sample collection and treatment bottle according to any one of claims 1 to 10, comprising the steps of:

a) collecting samples: the user opens the outer cover and collects the biological sample in a closed container;

b) pre-treatment of the sample: the liquid is sealed in the biological sample collection and treatment bottle; the treatment liquid is released from the closed environment by other physical or chemical means such as extrusion or pushing;

c) Observation of the effect of sample pretreatment: If necessary, the physical properties of the internal sample can be observed by the naked eye, and the user has a good user experience;

d) Transfer of the sample: open the outlet cover and output the treated liquid by extrusion or push-up; the pathogenic microorganisms in the sample are killed due to the pre-sample treatment, and the release of the treated liquid reduces the pollution environment and infection operation. Risk.
The biological sample collection and treatment bottle according to claim 1, wherein in the step b), the sample and the treatment reagent are mixed without opening the treatment bottle to complete the sample preparation.
The biological sample collection and treatment bottle according to claim 1, wherein in the step c), the sample properties and the treatment process can be observed without opening the treatment bottle.
The biological sample collection processing bottle according to claim 1, wherein said preprocessed sample is output without said external tool in said step d).