CN107828639B - Sample injector with pretreatment function and sample pretreatment and sample injection method - Google Patents
Sample injector with pretreatment function and sample pretreatment and sample injection method Download PDFInfo
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- CN107828639B CN107828639B CN201711182063.3A CN201711182063A CN107828639B CN 107828639 B CN107828639 B CN 107828639B CN 201711182063 A CN201711182063 A CN 201711182063A CN 107828639 B CN107828639 B CN 107828639B
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
Abstract
The invention provides a sample injector with a pretreatment function, and a sample pretreatment and sample injection method. After the sample treatment is finished, subsequent accurate quantification and micro-pipetting can be directly carried out, and the treated sample liquid is directly quantitatively added into a subsequent detection reagent, so that the subsequent detection is finished. Therefore, the pretreatment of the pre-loaded reagent to the sample and the pipetting and sample adding can be finished without checking professional instruments in a user laboratory, and the operation process is simple and convenient. And important support is provided for realizing omnibearing autonomous detection.
Description
Technical Field
The invention relates to the technical field of biological inspection, in particular to the technical fields of clinical inspection, forensic inspection, blood product inspection, food inspection and the like, and particularly relates to a sample injector with a pretreatment function and a sample pretreatment and sample injection method.
Background
The genetic testing is a process of finding abnormality of a specific genetic material by performing testing and analysis using genetic and molecular biological techniques. In recent thirty years, molecular biology technology has been developed rapidly, and gene detection has been widely applied in clinical examination, precise medical treatment, food inspection and quarantine, species identification and the like.
In general, gene detection is based on gene amplification techniques such as PCR, and includes PCR-fluorescent probe method, PCR-hybridization method, gene chip method, and the like. In application, a gene detection reagent manufacturer generally provides a detection kit, professional organizations such as medical institution inspection departments, food inspection and quarantine departments and the like use the detection kit provided by the manufacturer to detect a sample to be detected, and the organizations feed back a detection structure to users submitting inspection applications. The method comprises three steps of taking the inspection result obtained by an inspection client as a terminal point, providing a reagent by a manufacturer, executing inspection by an inspection mechanism, and feeding the inspection result back to a user by the inspection mechanism. The most important inspection link needs to depend on the professional operation of a quality-controlled gene amplification laboratory, a professional instrument of the laboratory and a trained laboratory, generally needs a longer inspection period, and a report issuing of an inspection mechanism also needs a certain period, so that the total period for obtaining an inspection result by a user is longer.
In the market, the existing corresponding products are designed and produced corresponding to the process. Therefore, a diagnostic kit provided by a manufacturer of the diagnostic kit to a user (often a detection mechanism) usually comprises a sample treatment reagent and a detection reagent, and the packaging materials of the two reagents are common containers and do not comprise a liquid-transferring instrument.
The diagnostic reagent executing test process is generally divided into two steps of sample treatment and test, because the pipe of the sample treatment liquid is only a common pipe and does not have a liquid separation function, the pretreated sample treatment liquid needs to be sucked by a pipette in a laboratory and then transferred into a detection reagent reaction tube for subsequent detection reaction. The use of such reagents is particularly restrictive for testing the experimental conditions of the user, requiring a test laboratory with specialized high precision pipettes, the operation of which also requires specialized training.
In the field of biological testing technology, "autonomous detection" is becoming a subject of increasing attention and importance. At present, professional personnel in the industry design special sampling instruments to support clients to automatically operate and complete sampling, but the links such as sample treatment and pipetting still need to leave professional personnel, professional instruments and professional environments.
Disclosure of Invention
In order to solve the problem that the quantitative transfer of a sample treatment solution cannot be completed by the conventional detection kit without a liquid transfer device in an inspection laboratory in the sample detection process, the invention provides a sample injector with a pretreatment function, and a sample pretreatment and sample injection method. After the sample treatment is finished, subsequent accurate quantification and micro-pipetting can be directly carried out, and the treated sample liquid is directly quantitatively added into a subsequent detection reagent, so that the subsequent detection is finished. Therefore, the pretreatment of the pre-loaded reagent to the sample and the pipetting and sample adding can be finished without checking professional instruments in a user laboratory, and the operation process is simple and convenient. And important support is provided for realizing omnibearing autonomous detection.
The technical scheme adopted by the invention is as follows:
an applicator with pre-processing functionality, comprising:
the bottle body is provided with a cavity for accommodating a sample processing reagent and a bottle opening for communicating the cavity with the outside;
the liquid transfer bottle cap is sealed at the bottle mouth and provided with a top part, the top part is provided with a quantitative liquid transfer pump and comprises a pump core with a liquid transfer channel which is normally in a closed state, and when the pump core is pressed, the liquid transfer channel is communicated with the cavity and the outside of the bottle body;
a pressing unit connected to and for pressing the pump core.
Furthermore, the pressing unit is provided with a cover body for accommodating all or part of the pipette cap, one end of the cover body is an open end, the other end of the cover body is a semi-closed end, a channel-shaped interface is formed at the semi-closed end, and the pressing unit is connected with the pump core in a buckling manner through one end, located in the cover body, of the channel-shaped interface.
Furthermore, one end of the channel-shaped interface, which is positioned outside the cover body, is provided with a fixed connecting structure, and the pressing unit is connected with a liquid-transferring needle head through the fixed connecting structure.
Further, the device also comprises a protective cap buckled on the pipetting needle head.
Further, at least one clamping groove is formed above the top of the liquid transfer bottle cap, at least one clamping plate corresponding to the clamping groove is formed inside the cover body, and when the pressing unit is connected with the pump core, the clamping plate is inserted into the clamping groove to form a linkage structure.
Furthermore, the outer wall of the cover body is provided with anti-skid grains.
Further, the liquid-transfering bottle lid is connected with the bottle body through a thread structure to seal the bottle mouth.
Furthermore, the pump core is a quantitative pump core with liquid outlet precision of 25ul, 30ul and 50 ul.
Furthermore, the bottle body is made of a heat-resistant polymer material.
The sample pretreatment and sample adding method realized by the sample adding device comprises the following steps:
adding a sample into the sample processing reagent accommodated in the cavity, and sealing the bottle mouth;
pretreating a sample in the sample treatment solution according to certain reaction conditions to obtain a sample treatment solution;
the sample injector is placed with the bottle mouth downward or approximately downward, and the pump core is pressed to quantitatively and fractionally transfer the processed sample liquid to a subsequent processing container.
By adopting the technical scheme, only one appliance can be used for carrying out simple pretreatment such as uniform mixing and heating through pre-loading the sample treatment reagent, and subsequent sample treatment can be carried out without container transfer, for example, the liquid transfer or the liquid separation of the sample treatment liquid can be directly realized during the detection reaction of the detection reagent, a liquid transfer machine in a laboratory is not required to be used for carrying out the liquid transfer, the operation is simple and convenient, and the liquid outlet precision meets the detection reaction requirement. The high requirement on the traditional sample detection environment is reduced, the operation difficulty is reduced, and the time can be obtained without the operation of a professional, so that the 'autonomous detection' of the all-dimensional human body sample provides powerful support.
Drawings
Fig. 1 is a schematic structural diagram of a sample injector with preprocessing function according to an embodiment of the present invention.
FIG. 2 is a perspective view of a pipette cap in an applicator with a pretreatment function according to an embodiment of the present invention.
FIG. 3 is a top view of a pipette cap in an applicator with a pretreatment function according to an embodiment of the present invention.
FIG. 4 is a bottom view of a pipette cap in an applicator with pretreatment features according to an embodiment of the present invention.
Fig. 5 is a schematic sectional view taken along the line a-a in fig. 3.
FIG. 6 is a perspective view of a push button of the sample injector with preprocessing function according to an embodiment of the present invention.
FIG. 7 is a perspective view of another view of a push button of the sample injector with preprocessing function according to an embodiment of the present invention.
FIG. 8 is a schematic diagram of a push button of the sample injector with preprocessing function according to an embodiment of the present invention.
FIG. 9 is a block diagram of a push button of the sample injector with preprocessing function according to an embodiment of the present invention.
Fig. 10 is a schematic view of the cross-sectional structure along the direction B-B in fig. 9.
Fig. 11 is an enlarged view of the portion I in fig. 10.
Fig. 12 is an enlarged view of section II in fig. 10.
FIG. 13 is a perspective view of a bottle of the sample injector with a pre-treatment function according to an embodiment of the present invention.
FIG. 14 is a schematic diagram of a front view of a bottle of a sample injector with a pre-processing function according to an embodiment of the present invention.
FIG. 15 is a schematic cross-sectional view of a bottle of the sample injector with pretreatment function according to an embodiment of the present invention.
Detailed Description
The technical solution in the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present invention.
As shown in fig. 1, in an embodiment, there is provided a sampler with preprocessing function, including: the bottle body 1 is provided with a cavity for accommodating a sample processing reagent and a bottle mouth for communicating the cavity with the outside; the liquid transfer bottle cap 2 is sealed at the bottle mouth and is provided with a top part, the top part is provided with a quantitative liquid transfer pump 4 and comprises a pump core with a liquid transfer channel which is normally in a closed state, and when the pump core is pressed, the liquid transfer channel is communicated with the cavity and the outside of the bottle body; and a pressing unit connected to and for pressing the pump core.
The pump core is, for example, a high-precision quantitative 25ul pump core, so that the pressing type quantitative liquid outlet can be realized, and the liquid outlet precision of 25ul can be ensured.
In this embodiment, the pushing unit is, for example, a pushing button 3, as shown in fig. 6 to 12, the pushing button has a cover body for accommodating all or part of the pipette cap, one end of the cover body is an open end, the other end is a semi-closed end, the semi-closed end is formed with a channel-shaped interface, and the pushing button is connected with the pump core in a buckling manner through one end of the channel-shaped interface located inside the cover body. As shown in fig. 12, which is a structural schematic of one end of the channel-shaped interface located inside the cover.
The channel-shaped interface is provided with a fixed connecting structure at one end positioned outside the cover body, and the push button is connected with the liquid-transferring needle head 5 through the fixed connecting structure. With particular reference to fig. 11, the pipetting needle is attached by an internally threaded conical locking fitting that fits into the pipetting needle. The protective cap 6 is buckled on the liquid-transferring needle head 5 to play the role of safety protection and the role of isolating the outside to prevent pollution.
The pipetting bottle cap with the dosing pump embedded in the center can be screwed with the bottle body to form a closed container, and sample processing operations such as mixing and heating of contents are supported. This constant delivery pump central authorities are a long and thin liquid channel, and inside is in airtight state when not pressing the pump core, and the liquid that isolated bottle was built-in is connected with the external world, and the volume change of inside airtight cavity when pressing the pump core causes pressure variation, makes the liquid channel of constant delivery liquid from pump core central authorities reserve, and this structure realizes the basis of quantitative minute liquid, application of sample. Particularly, interface shape interface and pump core adaptation, interface inner wall cover establish pump core outer wall and form the cooperation, and the interface has the effect of the through-hole of intercommunication pump core, and the two is connected and form the cooperation back, and vertical downward pressing at this moment pushes away the button, pushes away the pressure transmission of button and give the pump core, can pump the liquid ration in the bottle out, discharge from pushing away the upper outlet of button through the pump core through the effect of ration pump. By means of the structure, the liquid can be transferred by the sample injector, and the liquid in the bottle body can be quantitatively transferred to the reaction tube for detecting the reagent without a laboratory liquid transfer device.
The pump-in and pump-out are based on aerodynamics, when the pump core is extruded, the extrusion body space inside the pump core is extruded, redundant air is discharged, the inside of the pump core still keeps 1 atmosphere, when a hand is loosened, the space is stretched and released instantly, instant vacuum and negative pressure can occur inside the pump core, the negative pressure can suck quantitative liquid instantly, the pump core is extruded again to pump out the liquid, and then the hand is loosened and … is sucked again for circulation.
And the ration pump core of current product is selected for use to this embodiment, and the technical purpose of this application is realized to the product that field technical personnel can select corresponding precision according to the demand, and the structural principle is similar, and the ration of concrete pump core can be adjusted based on different demands, does not give unnecessary details here.
As shown in fig. 2 to 5, at least one clamping groove is formed above the top of the pipette cap, at least one clamping plate corresponding to the clamping groove is formed inside the cover body of the push button, and when the channel-shaped interface of the push button is connected with the pump core, the clamping plate is inserted into the clamping groove to form a linkage structure.
The liquid-transfering bottle lid is connected with the bottle body through a thread structure to seal the bottle mouth. The bottle body structure is shown in fig. 13 to 15. In addition, the outer wall of the cover body is formed with anti-skidding lines, so that the liquid-transfering bottle cap can be driven to rotate by twisting the push button, and the thread screwing structure of the bottle mouth of the liquid-transfering bottle cap and the bottle body can be removed.
The bottle body is made of a material which can withstand a certain heating without deformation or deterioration, and is made of a heat-resistant polymer material such as a PP material. Of course, other stable materials with heat resistance can be selected according to the requirement, and are not described in detail herein.
The practical application of the sample injector described in the above embodiments is mainly embodied in two scenarios, namely a pre-reagent scenario of a detection reagent manufacturer and a scenario in which a detection reagent performs an inspection.
In the scene of pre-installation reagent, operating personnel will add the appearance ware push away the button on the bottle be fixed in the operation panel down, will push away button counter-clockwise turning, push away the vertical card of the inside screens in button top in the draw-in groove of pipetting bottle lid upper end, make and push away the linkage of button main part and pipetting bottle lid, push away the button rotation and can drive the pipetting bottle lid rotatory to open the pipetting bottle lid of connecting on the bottle, can carry out subsequent reagent filling. And after the reagent is filled, the push button and the liquid-transfering bottle cap are screwed on the screw bottle body clockwise.
In a detection scene, an inspector needs to perform sample processing in the first step, open the bottle cap by adopting the operation which is the same as the filling and opening mode, add the collected sample into a pre-loaded reagent in the cavity inside the bottle body, and close the cap according to the closing mode. And then the mixture can be uniformly mixed, and can also be placed into instruments such as a metal bath and a water bath which are matched with the bottle body for heating treatment. After the pretreatment, the second sample treatment liquid is pipetted, the sample injector is directly inverted, the injection needle is downward, the push button is pressed for several times to discharge the air at the front end, after liquid can be uniformly discharged, the push button is gradually and fully pressed downward corresponding to a reaction system, when the push button is pressed downward, due to the fact that the channel-shaped interface inside the push button is tightly matched with the pump core of the quantitative pump, pressure is transmitted to the pump core, the built-in sample treatment liquid is quantitatively pumped out, and the pumped quantitative liquid is discharged through the pump core and a connecting channel of the push button. The operation can directly realize quantitative sample adding in a detection system, and participate in subsequent detection reaction without using a laboratory pipettor.
Foretell detection reaction scene, if the package material of follow-up detect reagent is soft tube caps such as butyl rubber stopper, silica gel, can install the liquid-transfering syringe needle on pushing away the internal thread circular cone locking joint on the button, the ware that adds can utilize the penetrability of syringe needle directly to penetrate inside the package material of detect reagent, presses and pushes away the button and accomplish the ration application of sample. After the sample is added to the detection reagent, the detection reaction can be completed according to the conventional operation. The complicated operation of repeatedly opening and closing the cover can be avoided.
It is to be understood that the described embodiments are merely exemplary of the invention, and not restrictive of the full scope of the invention. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Claims (7)
1. An applicator with preprocessing, comprising:
the bottle body is provided with a cavity for accommodating a sample processing reagent and a bottle opening for communicating the cavity with the outside;
the liquid transfer bottle cap is sealed at the bottle mouth and provided with a top part, the top part is provided with a quantitative liquid transfer pump and comprises a pump core with a liquid transfer channel which is normally in a closed state, and when the pump core is pressed, the liquid transfer channel is communicated with the cavity and the outside of the bottle body;
the pressing unit is connected with the pump core and used for pressing the pump core, the pressing unit is provided with a cover body for accommodating all or part of the pipetting bottle cap, one end of the cover body is an open end, the other end of the cover body is a semi-closed end, a channel-shaped interface is formed at the semi-closed end, and the pressing unit is connected with the pump core in a buckling manner through one end of the channel-shaped interface, which is positioned in the cover body; one end of the channel-shaped interface, which is positioned outside the cover body, is provided with a fixed connecting structure, and the pressing unit is connected with a liquid-transferring needle head through the fixed connecting structure;
the pump core is selected from quantitative pump cores with liquid outlet precision of 25ul, 30ul and 50 ul.
2. The applicator with pretreatment function of claim 1, further comprising a protective cap attached to said pipetting tip.
3. The sample injector with pretreatment function as claimed in claim 1, wherein at least one slot is formed above the top of the pipette cap, at least one catch plate corresponding to the slot is formed inside the housing, and when the pressing unit is connected to the pump core, the catch plate is inserted into the slot to form a linkage structure.
4. The sample injector with pretreatment function of claim 1, wherein the outer wall of the housing is formed with an anti-slip texture.
5. The sample injector with pretreatment function as claimed in claim 1, wherein the pipette cap is connected with the bottle body through a thread structure to close the bottle mouth.
6. The sample injector with pretreatment function of claim 1, wherein the bottle body is made of heat-resistant polymer material.
7. Sample pretreatment, loading method by means of the applicator according to any of claims 1 to 6, comprising the following steps:
adding a sample into the sample processing reagent accommodated in the cavity, and sealing the bottle mouth;
pretreating a sample in the sample treatment solution according to certain reaction conditions to obtain a sample treatment solution;
the sample injector is placed with the bottle mouth downward or approximately downward, and the pump core is pressed to quantitatively and fractionally transfer the processed sample liquid to a subsequent processing container.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201711182063.3A CN107828639B (en) | 2017-11-23 | 2017-11-23 | Sample injector with pretreatment function and sample pretreatment and sample injection method |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201711182063.3A CN107828639B (en) | 2017-11-23 | 2017-11-23 | Sample injector with pretreatment function and sample pretreatment and sample injection method |
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| CN107828639A CN107828639A (en) | 2018-03-23 |
| CN107828639B true CN107828639B (en) | 2020-12-15 |
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN203253456U (en) * | 2013-05-30 | 2013-10-30 | 成都乾坤动物药业有限公司 | Multipurpose handheld adjustable constant-volume pipetting device |
| CN107202725A (en) * | 2017-07-18 | 2017-09-26 | 杭州优思达生物技术有限公司 | Biological specimen collection handles bottle and its application method |
| CN206577780U (en) * | 2016-12-07 | 2017-10-24 | 重庆中元生物技术有限公司 | A kind of portable micro sampling dilution Dropping feeder |
| CN207646214U (en) * | 2017-11-23 | 2018-07-24 | 北京海普威生物技术有限公司 | Sample injector with preprocessing function |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP3111976A1 (en) * | 2015-07-02 | 2017-01-04 | Cliniclab S.L. | Device for ejecting a hypodermic needle from a needle holder or syringe and corresponding blood sampling device |
| CN105628948B (en) * | 2016-03-04 | 2018-03-30 | 深圳普门科技有限公司 | A kind of high speed c reactive protein analyzer and its analysis method |
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2017
- 2017-11-23 CN CN201711182063.3A patent/CN107828639B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN203253456U (en) * | 2013-05-30 | 2013-10-30 | 成都乾坤动物药业有限公司 | Multipurpose handheld adjustable constant-volume pipetting device |
| CN206577780U (en) * | 2016-12-07 | 2017-10-24 | 重庆中元生物技术有限公司 | A kind of portable micro sampling dilution Dropping feeder |
| CN107202725A (en) * | 2017-07-18 | 2017-09-26 | 杭州优思达生物技术有限公司 | Biological specimen collection handles bottle and its application method |
| CN207646214U (en) * | 2017-11-23 | 2018-07-24 | 北京海普威生物技术有限公司 | Sample injector with preprocessing function |
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