CN107828639A - Sample injector and sample preprocessing, loading methods with preprocessing function - Google Patents
Sample injector and sample preprocessing, loading methods with preprocessing function Download PDFInfo
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- CN107828639A CN107828639A CN201711182063.3A CN201711182063A CN107828639A CN 107828639 A CN107828639 A CN 107828639A CN 201711182063 A CN201711182063 A CN 201711182063A CN 107828639 A CN107828639 A CN 107828639A
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- China
- Prior art keywords
- sample
- sample injector
- pump core
- bottle
- preprocessing function
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
Abstract
The present invention provides a kind of sample injector and sample preprocessing, loading methods with preprocessing function, and the sample injector can be with pre-filled reagent, and its material and structure can be after sample adds pre-filled reagent, the mixing and heating of support sample.After sample process is completed, follow-up accurate quantification, micropipette can also be directly carried out, directly treated sample liquid is quantitatively adding in follow-up detection reagent, so as to complete subsequent detection.Thus without examining the professional utensil can of customer laboratory to complete pre-treatment of the pre-filled reagent to sample, liquid relief sample-adding, and operating process is easy.Autonomous detection to realize comprehensive provides important support.
Description
Technical field
The present invention relates to bio-assay technology field, more particularly to clinical examination, forensic medical examination, blood product inspection, food
The fields such as product inspection technology, and in particular to a kind of sample injector and sample preprocessing, loading methods with preprocessing function.
Background technology
Genetic test is detected, analyzed using science of heredity, Protocols in Molecular Biology, so as to find specific hereditary thing
The abnormal process of matter.Protocols in Molecular Biology high speed development in the last thirty years, genetic test is in clinical examination, precisely doctor
Treatment, Food Inspection quarantine, species discriminating etc. are widely used.
Usual genetic test based on the gene amplifications such as PCR, including PCR- fluorescence probe methods, PCR- hybrid methods,
Gene chips etc..It is upper typically by gene detection reagent manufacturer offer detection kit in application, examined by medical institutions
The detection kit that the professional institutions such as section, Food Inspection quarantine departments are provided using producer performs the detection to measuring samples, machine
Structure will detect structural feedback to the user for submitting inspection request again.To examine Customer Acquisition assay as terminal, it is necessary to by
Producer provides reagent, inspection body and performs inspection, inspection body to user feedback assay, totally three links.It is wherein most heavy
The test stage wanted needs to rely on quality controlled gene magnification laboratory, the special instrument in laboratory and the experiment undergone training
The professional operation of member, generally requires longer round of visits, along with the report of providing of inspection body is also required to certain cycle,
So that the overall cycle that user obtains assay is longer.
On the market, existing corresponding product is all corresponding above-mentioned flow scheme design, production.Therefore the factory of diagnostic kit
Family, which is supplied in the diagnostic kit of user's (being often testing agency), generally comprises sample processing reagent and detection reagent, two kinds
The packaging material of reagent is all common vessel tool, and is had not comprising pipettor.
Diagnostic reagent performs checkout procedure and is generally divided into sample process and examines two steps, because the pipe of sample treatment solution
Material is common tubing, without liquid separation function, the sample treatment solution by pretreatment needs to be drawn with the pipettor in laboratory,
Transfer in detection reagent reaction tube and carry out follow-up detection reaction.Experiment bar of the application of this kind of reagent for inspection user
Part is to have specific limitation to require, it is necessary to which the high-precision pipettor for examining laboratory to possess specialty, its operation are also required to specialty
Training.
And turn into problem that is more and more concerned and paying attention in bio-assay technology field, " autonomous detection ".At present, it is existing
Industry personnel designs special sampling utensil, supports client's Self-operating to complete sampling, but for rings such as sample treatment and liquid reliefs
Section still needs to so be unable to do without professional, professional utensil and professional environment.
The content of the invention
In view of the above-mentioned problems, during sample detection, it is existing in order to release for sample treatment and liquid relief link
The limitation of quantitative transfer of the detection kit as sample treatment solution can not be completed not by the pipettor for examining laboratory, this hair
It is bright that a kind of sample injector and sample preprocessing, loading methods with preprocessing function are provided, the sample injector can with pre-filled reagent, and
And its material and structure can support the mixing and heating of sample after sample adds pre-filled reagent.Complete at sample
After reason, follow-up accurate quantification, micropipette can also be directly carried out, is directly quantitatively adding treated sample liquid follow-up
Detection reagent in, so as to complete subsequent detection.Completed in advance thus without the professional utensil can of customer laboratory is examined
Pre-treatment of the installed reagents to sample, liquid relief sample-adding, and operating process is easy.To realize that it is important that comprehensive autonomous detection provides
Support.
The technical solution adopted in the present invention is：
A kind of sample injector with preprocessing function, including：
One bottle, there is the cavity and connection cavity and extraneous bottleneck of accommodating sample processing reagent；
Envelope is located at a liquid relief bottle cap of the bottleneck, has a top, and the top is provided with a certain amount of liquid relief pump, including
Pump core with normality for a pipetting channel of closed, when pressing the pump core, the pipetting channel connection cavity and bottle
It is outside；
Connect and to press the one of pump core pressing unit.
Further, the pressing unit has the cover body for accommodating all or part of liquid relief bottle cap, and one end of cover body is
Open end, the other end are semiclosed end, and semiclosed end passes through the passage shape interface formed with a passage shape interface, pressing unit
One end inside cover body is fastened and connected with pump core.
Further, one end that the passage shape interface is located at outside cover body has a fixed connection structure, presses unit
One liquid relief syringe needle is connected by the fixed connection structure.
Further, in addition to button is located at a protective cap of the liquid relief syringe needle.
Further, the over top of the liquid relief bottle cap is formed with an at least neck, and the cover body has been internally formed pair
Should neck an at least clamp, for pressing unit when be connected with pump core, the clamp inserts neck composition linkage structure.
Further, the outer wall of the cover body is formed with non-slip texture.
Further, the liquid relief bottle cap is connected the closing bottleneck by helicitic texture with bottle.
Further, the pump core is the quantitative pump core that liquid precision is 25ul, 30ul, 50ul.
Further, the material of the bottle is resistance to hot polymeric material.
Sample preprocessing, the loading methods realized by above-mentioned sample injector, comprise the following steps：
Sample is added in the sample processing reagent housed in cavity, sealed a bottle；
Sample is pre-processed in sample treatment solution according to certain reaction condition, obtain sample treatment solution；
Make bottleneck downwards or approximate placement sample injector downwards, pressing pump core are quantitatively transferred to subsequently by several times by sample liquid is handled
Process container.
, not only can be by pre-installing sample processing reagent, so as to enter only by a utensil by taking above-mentioned technical proposal
The pretreatments such as the simple mixing of row, heating, also, container transfer need not be carried out, it becomes possible to subsequent samples processing is being carried out, such as
Liquid relief or the liquid separation of sample treatment solution are directly realized by during the detection reaction of detection reagent, the pipettor without reusing laboratory enters
Row liquid relief, it is easy to operate and go out liquid precision meet detection reaction require.Not only reduce the height for traditional pattern detection environment
It is required that and reduce operation difficulty, it is not necessary to by professional's operation can the time, make the " autonomous of comprehensive human sample
Detection " provides strong support.
Brief description of the drawings
Fig. 1 is the structural representation of the sample injector with preprocessing function in one embodiment of the invention.
Fig. 2 is the three-dimensional structure diagram of liquid relief bottle cap in the sample injector with preprocessing function in one embodiment of the invention.
Fig. 3 is the overlooking structure figure of liquid relief bottle cap in the sample injector with preprocessing function in one embodiment of the invention.
Fig. 4 is the face upwarding assumption diagram of liquid relief bottle cap in the sample injector with preprocessing function in one embodiment of the invention.
Fig. 5 is A-A profile structure diagram in Fig. 3.
Fig. 6 is the three-dimensional structure diagram of push button in the sample injector with preprocessing function in one embodiment of the invention.
Fig. 7 is the stereochemical structure at another visual angle of push button in the sample injector with preprocessing function in one embodiment of the invention
Figure.
Fig. 8 is the main structure figure of push button in the sample injector with preprocessing function in one embodiment of the invention.
Fig. 9 is the body structure chart of bowing of push button in the sample injector with preprocessing function in one embodiment of the invention.
Figure 10 is B-B cross-section structure diagram in Fig. 9.
Figure 11 is I portions enlarged diagram in Figure 10.
Figure 12 is II portions enlarged diagram in Figure 10.
Figure 13 is the three-dimensional structure diagram of bottle in the sample injector with preprocessing function in one embodiment of the invention.
Figure 14 is the main structure figure of bottle in the sample injector with preprocessing function in one embodiment of the invention.
Figure 15 is the cross-sectional view of bottle in the sample injector with preprocessing function in one embodiment of the invention.
Embodiment
Below in conjunction with the accompanying drawing in the embodiment of the present invention, the technical scheme in the embodiment of the present invention is carried out clear, complete
Whole description.
As shown in figure 1, in one embodiment, there is provided a kind of sample injector with preprocessing function, including：Bottle 1, has
Cavity and connection cavity and the extraneous bottleneck of accommodating sample processing reagent；Envelope has a top located at the liquid relief bottle cap 2 of bottleneck
Portion, top are provided with quantitative liquid relief pump 4, including the pump core with normality for the pipetting channel of closed, when pressing pump core, liquid relief
Passage is connected outside cavity and bottle；Connect and to press the pressing unit of pump core.
Wherein, pump core is, for example, high-precision quantitative 25ul pump cores, it is possible to achieve push type quantitative liquid-discharging, and can ensure
25ul's goes out liquid precision.
In the present embodiment, pressing unit is, for example, push button 3, and with reference to shown in Fig. 6 to Figure 12, push button, which has, accommodates whole or portion
Divide the cover body of liquid relief bottle cap, one end of cover body is open end, and the other end is semiclosed end, and semiclosed end connects formed with a passage shape
Mouthful, push button is fastened and connected by one end inside cover body of the passage shape interface with pump core.As shown in figure 12, as passage
The structural representation of one end inside cover body of shape interface.
One end that passage shape interface is located at outside cover body has a fixed connection structure, and push button passes through the fixed connection structure
Connect liquid relief syringe needle 5.With specific reference to Figure 11, liquid-transfering needle is connected by the internal thread cone locking joint being adapted to liquid relief syringe needle
Head.Button prevents pollution located at the protective cap 6 of liquid relief syringe needle 5 to play a part of safety protection function and the isolation external world.
The liquid relief bottle cap that center embedded in constant displacement pump can be screwed up with bottle as closed container, support content mixing,
Heat equal samples processing operation.The constant displacement pump center is an elongated fluid passage, when pump core is not pressed inside
In air-tight state, the built-in liquid of isolation bottle is connected with the external world, and when pressing pump core, the Volume Changes of inside airtight cavity are made
Into pressure change, quantitative liquid is reserved from the fluid passage in pump core center, the structure is to realize quantitative liquid separating, the base of sample-adding
Plinth.Specifically, interface shape interface is adapted to pump core, and interface inwall is arranged pump core outer wall and forms cooperation, and interface has connection
The effect of the through hole of pump core, after the two connects and forms cooperation, push button, the pressure transmission of push button are now pressed straight down to pump
Core, the liquid quantitative in bottle can be pumped out by the effect of constant displacement pump, the discharge suitable for reading by pump core from push button.By this
Structure, liquid relief can be realized in itself with sample injector, the liquid quantitative in bottle be moved in the reaction tube of detection reagent, without logical
The pipettor for crossing laboratory is realized.
It is pumped into and pumps out and be all based on aerodynamics, when extruding pump core, the pressing body space inside pump core is squeezed
Pressure, unnecessary air is discharged, and is also to maintain 1 atmospheric pressure inside it, internal when hand unclamps, space moment is stretched, discharges
Vacuum, the negative pressure of a moment occurs, negative pressure can suck quantitative liquid, extrude this partially liq is got again again moment
Go, then loose one's grip, inhale ... circulation again.
And the present embodiment selects the quantitative pump core of existing product, those skilled in the art can select corresponding essence according to demand
Degree, the similar product of structural principle realize the technical purpose of the application, and specific pump core can quantitatively be entered based on different demands
Row adjustment, will not be repeated here.
As shown in Figures 2 to 5, the over top of liquid relief bottle cap is internally formed formed with an at least neck, the cover body of push button
Have to should neck an at least clamp, when the passage shape interface of push button is connected with pump core, clamp insertion neck form linkage knot
Structure.
Liquid relief bottle cap is connected by helicitic texture with bottle to seal a bottle.Bottle structure is as shown in FIG. 13 to 15.In addition,
The outer wall of cover body is easy to drive liquid relief bottle cap to rotate by reversing push button formed with non-slip texture, thus release liquid relief bottle cap with
The threaded engagement structure of the bottleneck of bottle.
The material of bottle is required to bear necessarily heating and does not produce deformation, qualitative change, for example, resistance to hot polymeric material, such as
PP materials.Certainly there is the stabilizing material of heat resistance can also select as needed for other, will not be repeated here.
The practical application of sample injector described by above-described embodiment is mainly reflected in two scenes, detection reagent factory
The pre-filled reagent scene and detection reagent of family perform the scene examined.
In the scene of pre-filled reagent, the push button of sample injector is fixed on operating desk by operating personnel down towards upper bottle body,
By push button rotate counterclockwise, the inside screens of push button top is stuck in the neck of liquid relief bottle cap upper end vertically, makes push button main body and liquid relief
Bottle cap links, and push button rotation can drive liquid relief bottle cap to rotate, and so as to which the liquid relief bottle cap being connected on bottle be opened, can carry out
Follow-up reagent is filling.After the completion of reagent is filling, then push button is screwed up on screw socket bottle together with liquid relief bottle cap clockwise.
In scene is detected, reviewer's first step will carry out sample process, using with it is above-mentioned it is filling uncap by the way of phase
Bottle cap is opened in same operation, and the sample of collection is added in the pre-filled reagent in bottle internal cavities, lid mode is closed according to above-mentioned
Close lid.It can be mixed afterwards, can also be put into do in the instruments such as the metal bath being adapted to bottle, water-bath and heat.In advance
Processing carries out second sample treatment solution liquid relief after terminating, and is directly inverted this sample injector, and down, pressing push button is for several times for injection needle
Front end air is discharged, after it uniformly can go out liquid, corresponding to reaction system, gradually fully presses down on push button, push button is downward
During pressing, because of the passage shape interface inside push button and constant displacement pump pump core tight fit, pressure transmission is to pump core, at built-in sample
Reason liquid is quantitatively pumped out, and the quantitative liquid pumped out is discharged by the interface channel of pump core and push button.Aforesaid operations can be directly realized by
Quantitatively it is loaded, participates in follow-up inspection reaction, without reusing laboratory pipettor into detection architecture.
Above-mentioned detection reaction scene, can if the packaging material of subsequent detection reagent is the soft lids such as butyl rubber plug, silica gel
To install liquid relief syringe needle on the internal thread cone locking joint on push button, sample injector can utilize the penetrability of syringe needle directly to wear
Enter inside the packaging material of detection reagent, pressing push button completes quantitative sample-adding.After sample is added in detection reagent, can routinely it operate
Complete detection reaction.The troublesome operation of open-close lid can be avoided repeatedly.
Obviously, described embodiment is only part of the embodiment of the present invention, rather than whole embodiments.Based on this hair
Embodiment in bright, the every other implementation that those of ordinary skill in the art are obtained under the premise of creative work is not made
Example, belongs to the scope of protection of the invention.
Claims (10)
- A kind of 1. sample injector with preprocessing function, it is characterised in that including：One bottle, there is the cavity and connection cavity and extraneous bottleneck of accommodating sample processing reagent；Envelope has a top located at a liquid relief bottle cap of the bottleneck, and the top is provided with a certain amount of liquid relief pump, including with Normality is the pump core of a pipetting channel of closed, and when pressing the pump core, the pipetting channel is connected outside cavity and bottle；Connect and to press the one of pump core pressing unit.
- 2. there is the sample injector of preprocessing function as claimed in claim 1, it is characterised in that the pressing unit, which has, to be accommodated One cover body of all or part of liquid relief bottle cap, one end of cover body are open end, and the other end is semiclosed end, semiclosed end formed with One passage shape interface, pressing unit are fastened and connected by one end inside cover body of the passage shape interface with pump core.
- 3. there is the sample injector of preprocessing function as claimed in claim 2, it is characterised in that the passage shape interface is positioned at cover One end outside body has a fixed connection structure, and pressing unit connects a liquid relief syringe needle by the fixed connection structure.
- 4. there is the sample injector of preprocessing function as claimed in claim 3, it is characterised in that also include button and be located at the liquid relief One protective cap of syringe needle.
- 5. there is the sample injector of preprocessing function as claimed in claim 2, it is characterised in that on the top of the liquid relief bottle cap Side formed with an at least neck, the cover body be internally formed to should neck an at least clamp, pressing unit and pump core connect When connecing, the clamp insertion neck forms linkage structure.
- 6. as claimed in claim 2 have preprocessing function sample injector, it is characterised in that the outer wall of the cover body formed with Non-slip texture.
- 7. there is the sample injector of preprocessing function as claimed in claim 1, it is characterised in that the liquid relief bottle cap passes through screw thread Structure is connected the closing bottleneck with bottle.
- 8. there is the sample injector of preprocessing function as claimed in claim 1, it is characterised in that the pump core is selected from out liquid precision For 25ul, 30ul, 50ul quantitative pump core.
- 9. there is the sample injector of preprocessing function as claimed in claim 1, it is characterised in that the material of the bottle is heat-resisting Polymeric material.
- 10. sample preprocessing, the loading methods realized by any one of claim 1 to 9 sample injector, including following step Suddenly：Sample is added in the sample processing reagent housed in cavity, sealed a bottle；Sample is pre-processed in sample treatment solution according to certain reaction condition, obtain sample treatment solution；Make bottleneck downwards or approximate placement sample injector downwards, pressing pump core are quantitatively transferred to subsequent treatment by several times by sample liquid is handled Container.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711182063.3A CN107828639B (en) | 2017-11-23 | 2017-11-23 | Sample injector with pretreatment function and sample pretreatment and sample injection method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711182063.3A CN107828639B (en) | 2017-11-23 | 2017-11-23 | Sample injector with pretreatment function and sample pretreatment and sample injection method |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN107828639A true CN107828639A (en) | 2018-03-23 |
| CN107828639B CN107828639B (en) | 2020-12-15 |
Family
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201711182063.3A Active CN107828639B (en) | 2017-11-23 | 2017-11-23 | Sample injector with pretreatment function and sample pretreatment and sample injection method |
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2017
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| CN203253456U (en) * | 2013-05-30 | 2013-10-30 | 成都乾坤动物药业有限公司 | Multipurpose handheld adjustable constant-volume pipetting device |
| EP3111976A1 (en) * | 2015-07-02 | 2017-01-04 | Cliniclab S.L. | Device for ejecting a hypodermic needle from a needle holder or syringe and corresponding blood sampling device |
| CN105628948A (en) * | 2016-03-04 | 2016-06-01 | 深圳普门科技有限公司 | High-speed C reactive protein analyzer and analyzing method thereof |
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| CN107828639B (en) | 2020-12-15 |
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