CN210584971U - Pretreatment reaction device - Google Patents
Pretreatment reaction device Download PDFInfo
- Publication number
- CN210584971U CN210584971U CN201921812473.6U CN201921812473U CN210584971U CN 210584971 U CN210584971 U CN 210584971U CN 201921812473 U CN201921812473 U CN 201921812473U CN 210584971 U CN210584971 U CN 210584971U
- Authority
- CN
- China
- Prior art keywords
- reaction tube
- reaction
- tube
- thin wall
- sample
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Images
Landscapes
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
The utility model belongs to the technical field of detection, in particular to a sample pretreatment reaction device, which comprises a reaction tube, wherein the upper part of the reaction tube is a first reaction tube, and the lower part of the reaction tube is a second reaction tube, and the reaction device is characterized in that the first reaction tube is movably connected with the second reaction tube in a sealing way, and the second reaction tube is internally provided with a thin wall, so that the first reaction tube and the thin wall form a first sealed containing cavity, and the second reaction tube and the thin wall form a second sealed containing cavity; the lower part of the first reaction tube is a tip part extending towards the direction of the thin wall, and external force is applied to shorten the relative distance between the tip part and the thin wall and puncture the thin wall. The utility model discloses pretreatment reaction unit need not to be equipped with all the other professional utensil and professional operating personnel, can accomplish the preliminary treatment to the sample with the reagent of pre-installation, move liquid ration application of sample, and operation process is simple and convenient, provides important support for the realization of nucleic acid detection technique at basic level scene or the instant short-term test in scene.
Description
Technical Field
The utility model belongs to the technical field of detect, concretely relates to sample preliminary treatment reaction unit.
Background
In recent years, domestic infectious diseases and epidemic situations have been increasing year by year. For the detection of infectious diseases and epidemic situations, the molecular detection is a gold standard, and the sensitivity and the accuracy of the molecular detection are higher than those of an immunodetection method. However, the current nucleic acid detection needs to be supported by a central laboratory and professional instruments and personnel, so that the detection period is long, and even treatment is delayed or infection spread is caused. If a patient suspected of having influenza is detected, at least 1-2 days are required from the arrival at a sampling site, the sample is sent to a central laboratory, and the detection is carried out until the result is obtained, and the disease can rapidly progress or the infection spreads in the period of time; the African swine fever epidemic situation which is seriously popular in the year needs 5-7 days from sample sending to result obtaining of basic-level farms and slaughtering enterprises, detection results are not always waited for, pigs are already sick and dead, and the swine plague can be spread on a large scale without isolation in the period, so that the swine plague situation has more extensive influence. The on-site rapid detection method based on nucleic acid molecule diagnosis can greatly improve the speed and accuracy of on-site detection, which has great significance for epidemic control, prevention and treatment.
However, the detection method based on nucleic acid molecule requires sample pretreatment, and the conventional sample pretreatment method still has the problems of needing professional auxiliary equipment, complex extraction process, easy occurrence of cross contamination and the like, and on the other hand, the on-site rapid detection method based on nucleic acid molecule diagnosis provides higher requirements for the sample pretreatment method.
Disclosure of Invention
The utility model aims to solve the above problems existing in the prior art and provide a pretreatment reaction device with simple operation, low cost and accurate quantification. The pretreatment reaction device is pre-loaded with a reagent, and can directly perform subsequent accurate quantification and micro-pipetting after sample pretreatment is completed, so that the treated sample liquid is directly quantitatively added into subsequent detection equipment, and subsequent detection is completed. The device can fully mix the reagent in advance in the device, and guaranteed that accurate quantitative preliminary treatment product gets into the downstream reaction, guaranteed the accuracy of testing. The device does not need to be equipped with other professional instruments and professional operators, can finish the pretreatment of the sample and the pipetting and sample adding by using a pre-loaded reagent, has simple and convenient operation process, and provides important support for the realization of the instant and rapid detection of the nucleic acid detection technology in basic scenes or fields.
In order to solve the technical problem, the utility model adopts the following technical scheme:
a pretreatment reaction device comprises a reaction tube, wherein the upper part of the reaction tube is a first reaction tube, the lower part of the reaction tube is a second reaction tube, the first reaction tube is movably connected with the second reaction tube in a sealing way, a thin wall is arranged in the second reaction tube, so that the first reaction tube and the thin wall form a first sealed cavity, and the second reaction tube and the thin wall form a second sealed cavity; the lower part of the first reaction tube is a tip part extending towards the direction of the thin wall, and external force is applied to shorten the relative distance between the tip part and the thin wall and puncture the thin wall.
Preferably, the movable sealing connection mode is any one of threaded connection, inserting connection, rubber seal ring socket connection and the like.
Preferably, the pretreatment reaction device further comprises a sample outlet mechanism, wherein the sample outlet mechanism comprises a cylindrical sample outlet cylinder and a sample outlet; the inner diameter of the upper part of the sample outlet cylinder is slightly larger than the outer diameter of the lower part of the second reaction tube, the sample outlet cylinder is inserted into the second reaction tube and is in sealing connection with the second reaction tube, and the lower part of the sample outlet cylinder is provided with a sample outlet; and the lower part of the second reaction tube is provided with a hole, and external force is applied to shorten the relative distance between the second reaction tube and the sample outlet so as to extrude the content in the second reaction tube.
Preferably, the sample outlet mechanism further comprises a quantitative plug, the quantitative plug is located on the inner side of the sample outlet cylinder and right below the hole, is connected with the sample outlet cylinder in a non-sealing and fixed manner and forms a sample outlet channel with the sample outlet, the upper part of the quantitative plug further comprises a sealing element extending towards the outer side of the quantitative plug body, the outer diameter of the upper part of the quantitative plug is larger than the aperture of the hole, and the outer diameter of the lower part of the quantitative plug is smaller than the aperture of the hole; the sealing element is positioned in the second reaction tube, so that the second reaction tube in the initial position forms a sealed cavity.
Preferably, the quantitative plug is in threaded connection with the sample outlet cylinder, and a channel communicated with the sample outlet is arranged on the outer side of the plug body; the sealing element is in a cap shape and is integrally formed with the quantitative plug body.
Preferably, the seal further comprises a rubber seal.
Preferably, the upper part of the first reaction tube is movably connected with the tip part in a meshing manner.
Preferably, the upper part of the second reaction tube is a screwing tube, and the lower part of the second reaction tube is a protection tube; the upper part of the screwing pipe is movably connected with the upper part of the first reaction pipe in an inserting and embedding manner, the middle part of the screwing pipe is provided with external threads, the lower part of the screwing pipe is provided with internal threads, and the lower pipe orifice of the screwing pipe is provided with the thin wall; the middle part of the tip part is provided with an external thread matched with the internal thread of the screwed pipe; the protective tube is cylindrical, the upper portion of the protective tube is provided with an internal thread matched with the external thread of the screwing tube, and the outer portion of the upper tube wall of the protective tube is fixedly connected with a disposable fracture limiting ring.
Preferably, the upper end of the sample outlet cylinder is provided with a limiting flange extending outwards.
Preferably, the limiting flanging is fixedly connected with the one-time fracture limiting ring.
Preferably, the limiting flanging is fixedly connected with the one-time fracture limiting ring in a buckling mode.
Preferably, the sample outlet mechanism further comprises a sample outlet protective sleeve.
Preferably, the first reaction tube and the second reaction tube are pre-filled with a reagent.
Preferably, the tip part is a tube with a cutting edge or a sharp spike at the lower end.
Preferably, the one-time fracture limiting ring is fixedly connected with multiple points outside the pipe wall of the upper end of the protection pipe.
Preferably, the thin wall is made of any one of PP, PE and PVC.
Preferably, the thin wall is a thin film.
Preferably, the upper part of the reaction tube is in threaded connection with a tube cover.
Preferably, the upper part of the first reaction tube is provided with a strip-shaped anti-skid groove.
The utility model adopts the above technical scheme, following beneficial effect has:
1. the first reaction tube and the second reaction tube are in sealed movable connection, reaction reagents are pre-loaded, the tip parts and the thin walls for separating the first reaction tube from the second reaction tube are arranged in the two reaction tubes, when external force is applied, the tip parts pierce the thin walls, so that sample liquid to be detected enters the second reaction tube from the first reaction tube, two reactions before and after sample pretreatment are completed, the operation is simple, the ratio of the reagents in the first reaction tube to the reagents in the second reaction tube is ensured, and the pretreatment reaction can be smoothly completed. Different reagents can be preset to meet the requirements of pretreatment of different samples. The pretreatment environment is closed, cross contamination is avoided, and the method is wide in application range and applicable to the fields of clinical examination, forensic examination, blood product examination and the like.
2. The structure of the reaction tube and the sample outlet mechanism is designed based on the air pressure principle, the pretreated sample can be easily and accurately extruded quantitatively, the operation is simple, and the method is suitable for the detection method with the precision requirement on the sample amount.
3. The volume of the upper part of the quantitative plug is changed, so that the amount of the extruded sample is conveniently changed.
4. Through at the tip part, revolve to twist the pipe, the protection tube sets up corresponding screw thread and disposable fracture spacing collar for before accomplishing to pierce through the thin wall and mix first second reaction tube reagent, can't extrude the content with second reaction tube downstream earlier, avoid sample preliminary treatment failure, the device is scrapped, should prevent the maloperation and set up the greatly reduced operation mistake nature, more be applicable to various application scenarios.
5. The outer portion of the pipe wall of the upper end of the protection pipe is connected with the disposable fracture limiting ring in a multi-point fixed connection mode, the disposable fracture limiting ring and the limiting flanging are in a buckle type fixed connection mode, the protection pipe can be disconnected from the disposable fracture limiting ring by slightly twisting the device, the operation flow of twisting and pressing is guaranteed, and the operation difficulty is reduced.
6. The upper part of the first reaction tube is provided with a strip-shaped anti-skidding groove, so that the friction force is increased, and the twisting is convenient.
7. And a rubber sealing ring is added on the quantitative plug, so that the air tightness between the reaction tube and the sample outlet cylinder is further improved.
Drawings
Fig. 1 is a schematic sectional structure diagram of embodiment 1 of the present invention;
fig. 2 is a front view of embodiment 1 of the present invention;
fig. 3 is an exploded schematic view of embodiment 1 of the present invention;
fig. 4 is a front view of the exploded structure of embodiment 1 of the present invention;
fig. 5 is a sectional view of an exploded structure of embodiment 1 of the present invention;
FIG. 6 shows the result of agarose gel electrophoresis analysis of the RPA reaction of example 5 of the present invention;
FIG. 7 is a qPCR reaction diagram of example 5 of the present invention;
in the figure:
a tube cap 10, a first reaction tube upper part 11, a tip part 13, a first tooth column 14, a second tooth column 15, a cutting edge 16 and a first sealed containing cavity 17
The screw pipe 20, the protective pipe 21, the thin wall 22, the one-time fracture limiting ring 23 and the second sealed containing cavity 24
The sample outlet cylinder 31, the sample outlet 32, the quantifying plug 33, the limiting flange 34, the protective sleeve 35, the sealing element 36 and the channel 37
Detailed Description
Example 1.
Example 1.
The utility model relates to a pretreatment reaction device, which comprises a reaction tube and a sample outlet mechanism.
The upper part of the reaction tube is provided with a first reaction tube, the lower part of the reaction tube is provided with a second reaction tube, and the first reaction tube is movably connected with the second reaction tube in a sealing way.
One end of the upper part 11 of the first reaction tube is connected with a tube cap 10 by screw thread, the outside is provided with a strip-shaped anti-skid groove, the lower part is a tip part 13 extending towards the direction of the thin wall 22, the tip part 13 is hollow, the upper part is movably connected with the upper part 11 of the first reaction tube in a meshing way, and the lower part is a cutting edge 16.
The upper part of the second reaction tube is a screwed tube 20, and the lower part is a protection tube 21. The upper part of the screwing pipe 20 is movably connected with the upper part 11 of the first reaction pipe in an inserting and embedding way, the middle part of the screwing pipe is provided with external threads, the lower part of the screwing pipe is provided with internal threads, and the middle part of the tip part 13 is provided with external threads matched with the internal threads of the screwing pipe 20. The protection tube 21 is cylindrical, the upper portion of the protection tube is provided with an internal thread matched with the external thread of the screwing tube 20, and the outer portion of the upper tube wall of the protection tube is connected with a one-off fracture limiting ring 23 in a multi-point connection mode.
The thin wall 22 is arranged at the lower pipe opening of the screwing pipe 20 of the second reaction pipe, so that the first reaction pipe and the thin wall 22 form a first sealed containing cavity 17, the protection pipe 21 and the thin wall 22 in the second reaction pipe form a second sealed containing cavity 24, meanwhile, the lower part of the first reaction pipe is provided with the tip part 13 extending towards the direction of the thin wall 22, external force is applied to enable the lower part of the first reaction pipe to move towards the direction of the thin wall 22 in the second reaction pipe and puncture the thin wall 22, so that the liquids in the two sealed containing cavities are mixed, and the front reaction and the rear reaction of sample pretreatment are completed.
Through set up corresponding screw thread and disposable fracture spacing collar 23 at tip spare 13, revolve screwed pipe 20, protection tube 21, restriction second reaction tube moves downwards in advance and extrudes the content, leads to sample preliminary treatment failure, and the device is scrapped, greatly reduced operation degree of difficulty.
The outer portion of the upper end pipe wall of the protection pipe 21 is connected with the one-time fracture limiting ring 23 in a multi-point fixed connection mode, and the protection pipe 21 can be easily disconnected from the one-time fracture limiting ring 23.
The sample outlet mechanism comprises a cylindrical sample outlet cylinder 31 and a sample outlet 32. The inner diameter of the upper part of the sample outlet cylinder 31 is slightly larger than the outer diameter of the lower part of the second reaction tube, the sample outlet cylinder is inserted into the second reaction tube and connected with the second reaction tube in a sealing manner, the upper end of the sample outlet cylinder 31 is provided with a limiting flanging 34 extending outwards, the limiting flanging 34 is fixedly connected with the one-time fracture limiting ring 23 in a buckling manner, the lower part of the sample outlet cylinder is provided with a sample outlet 32, the lower part of the second reaction tube is provided with a hole, and external force is applied to enable the second reaction tube to move towards the direction of the sample outlet.
The sample outlet mechanism further comprises a quantitative plug 33, the quantitative plug 33 is located on the inner side of the sample outlet cylinder 31 and right below the second reaction tube hole and is in non-sealing fixed threaded connection with the sample outlet cylinder 31, the plug body is provided with a longitudinal channel 37 to form a sample outlet channel with the sample outlet 32, the upper end of the upper portion of the plug body is provided with a sealing element 36 extending outwards, the sealing element 36 is in a cap shape and is integrally formed with the quantitative plug 33, and the volume of the upper portion of the quantitative plug 33 can be changed, so that the amount of the extruded sample can be changed.
The sealing member 36 is located inside the reaction tube, so that the reaction tube in the initial position forms a sealed cavity, and the external force is applied to make the reaction tube move towards the direction of the sample outlet 32 to quantitatively extrude the content in the reaction tube.
The sample outlet mechanism further comprises a sample outlet 32 protective sleeve 35, so that the sample outlet 32 is prevented from being polluted before the liquid to be detected is extruded.
The first reaction tube and the second reaction tube are pre-filled with a reaction reagent.
The thin wall 22 may be made of any one of PP, PE, and PVC, or may be a thin film.
The utility model discloses the use step as follows:
(1) rotating to open the tube cap 10 and put a sample to be detected, then rotating to close the tube cap 10, shaking the pretreatment reaction device to uniformly mix the pre-loaded reaction reagent of the first reaction tube with the sample to be detected, and carrying out a first reaction;
(2) screwing the upper part 11 of the first reaction tube clockwise, wherein the upper part 11 of the first reaction tube drives the tip part 13 to generate a thread motion downwards through the first tooth column 14 and the second tooth column 15 to puncture the thin wall 22, the liquid of the sample to be detected of the first reaction tube flows into the second reaction tube, meanwhile, the tip part 13 drives the screwing tube 20 to rotate after the tip part 13 and the screwing tube 20 are completely screwed, the screwing tube 20 and the protection tube 21 are in a completely screwed state, the screwing tube 20 drives the protection tube 21 to rotate, and then the connection between the second reaction tube and the one-time fracture limiting ring 23 is disconnected.
(3) Shaking the pretreatment reaction device to uniformly mix the sample to be detected with the reaction reagent pre-loaded in the second reaction tube for reaction.
(4) And opening the protective sleeve 35, pressing the first reaction tube downwards to quantitatively extrude the sample liquid in the second reaction tube, and entering a detection link.
The experimental data for verifying the precise extrusion function of this example are shown in table 1.
And (3) testing conditions are as follows: the first reaction tube is pre-filled with 1000 microlitres of lysate; the second reaction tube was pre-filled with 1000. mu.L of diluent.
TABLE 1
Example 2.
The utility model relates to a preliminary treatment reaction unit, including the reaction tube, its upper portion of reaction tube is first reaction tube, and its lower part is the second reaction tube, first reaction tube and the sealed swing joint of second reaction tube, and the other end threaded connection of first reaction tube has the block, and the second reaction tube other end is the toper, is equipped with the hole to threaded connection has the hole cap.
The sealing movable connection mode is any one of threaded connection, inserting connection, rubber seal ring socket connection and the like.
The second reaction tube is internally provided with a thin wall, so that the first reaction tube and the thin wall form a first sealed cavity, and the second reaction tube and the thin wall form a second sealed cavity.
The thin wall can be made of any one of PP, PE and PVC, and can also be a thin film.
The lower part of the first reaction tube is a tip part extending towards the thin wall direction, and external force is applied to shorten the relative distance between the tip part and the thin wall and puncture the thin wall.
The tip member may be a tube having a cutting edge or spike at its lower end.
The first reaction tube and the second reaction tube are pre-filled with a reaction reagent.
The device has the following use process: opening the cap, adding a sample to be detected, enabling the reaction reagent pre-loaded in the first reaction tube to react with the sample to be detected, applying external force in a screwing or pressing mode after the reaction is completed to enable the relative distance between the tip part and the thin wall to be shortened, puncturing the thin wall, enabling the sample to be detected in the first reaction tube to enter the second reaction tube to react with the reaction reagent pre-loaded in the second reaction tube, and unscrewing the perforated cap after the reaction is completed to enable the content in the second reaction tube to flow out to enter the next link.
Example 3.
The utility model relates to a preliminary treatment reaction unit, including the reaction tube, its upper portion of reaction tube is first reaction tube, and its lower part is the second reaction tube, first reaction tube and the sealed swing joint of second reaction tube, and the other end threaded connection of first reaction tube has the block, and the second reaction tube other end is the toper, is equipped with the hole to threaded connection has the hole cap.
The sealing movable connection mode is any one of threaded connection, inserting connection, rubber seal ring socket connection and the like.
The first reaction tube is internally provided with a thin wall, so that the first reaction tube and the thin wall form a first sealed cavity, and the second reaction tube and the thin wall form a second sealed cavity.
The thin wall can be made of any one of PP, PE and PVC, and can also be a thin film.
The second reaction tube is provided with a tip part extending towards the thin wall direction at one end connected with the first reaction tube, and external force is applied to shorten the relative distance between the tip part and the thin wall and puncture the thin wall.
The tip member may be a tube having a cutting edge or spike at its lower end.
The first reaction tube and the second reaction tube are pre-filled with a reaction reagent.
The device has the following use process: opening the cap, adding a sample to be detected, enabling the reaction reagent pre-loaded in the first reaction tube to react with the sample to be detected, applying external force in a screwing or pressing mode after the reaction is completed to enable the relative distance between the tip part and the thin wall to be shortened, puncturing the thin wall, enabling the sample to be detected in the first reaction tube to enter the second reaction tube to react with the reaction reagent pre-loaded in the second reaction tube, and unscrewing the perforated cap after the reaction is completed to enable the content in the second reaction tube to flow out to enter the next link.
Example 4.
A pretreatment reaction device comprises a reaction tube and a sample outlet mechanism.
The sample outlet mechanism comprises a cylindrical sample outlet cylinder and a sample outlet.
The inner diameter of the upper part of the sample outlet cylinder is slightly larger than the outer diameter of the lower part of the reaction tube, and the sample outlet cylinder is inserted into the reaction tube and hermetically connected with the reaction tube.
The outer part of the upper pipe wall of the reaction pipe is fixedly connected with a disposable fracture limiting ring, and the lower part of the reaction pipe is provided with a hole.
The upper end of the sample outlet cylinder is provided with a limiting flanging extending outwards, the limiting flanging is fixedly connected with the one-time fracture limiting ring, and the lower part of the sample outlet cylinder is provided with a sample outlet.
Go out appearance mechanism and still include the ration stopper, the ration stopper is located the hole of a kind section of thick bamboo inboard and reaction tube under, with a kind section of thick bamboo non-seal fixed connection, non-seal fixed connection mode can be threaded connection, the cock body is equipped with vertical channel and goes out the appearance mouth and forms out the appearance passageway, its upper end is equipped with the sealing member of outside extension, the sealing member can be the cap form, with ration stopper cock body integrated into one piece, also can be the sealing washer of cover on the cock body, the accessible changes the volume on ration stopper upper portion, thereby change the sample volume of extruding.
The sealing element is positioned in the reaction tube, so that the reaction tube in the initial position forms a sealed cavity; the relative distance between the reaction tube and the sample outlet is shortened to the minimum by applying external force, and the content in the reaction tube is extruded quantitatively.
The sample outlet mechanism further comprises a sample outlet protective sleeve, and the sample outlet is prevented from being polluted before the liquid to be detected is extruded.
Example 5.
A pretreatment reaction apparatus was constructed in the same manner as in example 1.
The first reaction tube is pre-filled with 1mL of lysis solution, and the second reaction tube is pre-filled with diluent.
The use method of the embodiment comprises the following steps:
(1) respectively putting 4 samples of the oral swabs obtained by scraping off cells on two sides of the oral cavity of a human into a pretreatment reaction device, rotating to close a tube cover, and shaking the pretreatment reaction device to uniformly mix a reaction reagent pre-loaded in a first reaction tube with a sample to be detected of the oral swabs;
(2) standing for 5min, screwing the upper part of the first reaction tube clockwise, driving the tip part to generate thread movement downwards through the first tooth post and the second tooth post to puncture the thin wall, enabling the cracked sample to flow into the second reaction tube, driving the screwing tube to rotate by the tip part after the tip part and the screwing tube are completely screwed, driving the protection tube to rotate after the screwing tube and the protection tube are completely screwed, and further disconnecting the second reaction tube from the one-time fracture limiting ring;
(3) shaking the pretreatment reaction device to uniformly mix the sample to be detected of the oral swab with the reaction reagent pre-loaded in the second reaction tube for reaction;
(4) opening the protective sleeve, pressing the first reaction tube downwards with force, quantitatively extruding the sample liquid in the second reaction tube, extruding 50 mu L of sample liquid in the second reaction tube through the quantitative plug (at the moment, the treatment solution contains the cracked DNA), and entering the next detection link;
(5) the extruded sample liquid was used as a template, added to a Recombinase Polymerase Amplification (RPA) reaction, and reacted at 37 ℃ for 30 min. Finally, the RPA products were analyzed by agarose gel electrophoresis. The treated solution was added to a real-time quantitative fluorescence PCR (qPCR) reaction using the treated solution as a template, and the qPCR reaction was carried out while using the same sample extracted from a commercially available nucleic acid extraction kit as a control.
The results of the agarose gel electrophoresis analysis of the RPA reaction are shown in FIG. 6, and the oral swab sample treated by the device can be used for cracking DNA and performing the RPA reaction at normal temperature as a template. In addition, as shown in fig. 7, the qPCR reaction was performed using the buccal swab sample processed by the device as a template, and was not significantly different from the DNA sample (P) extracted by the commercial nucleic acid extraction kit.
The above description is only for the specific embodiments of the present invention, but the scope of the present invention is not limited thereto, and any changes or substitutions that are not thought of through the creative work should be covered within the scope of the present invention. Therefore, the protection scope of the present invention should be subject to the protection scope defined by the claims.
Claims (10)
1. A pretreatment reaction device comprises a reaction tube, wherein the upper part of the reaction tube is a first reaction tube, and the lower part of the reaction tube is a second reaction tube, and the pretreatment reaction device is characterized in that the first reaction tube is movably connected with the second reaction tube in a sealing way, and a thin wall is arranged in the second reaction tube, so that the first reaction tube and the thin wall form a first sealed containing cavity, and the second reaction tube and the thin wall form a second sealed containing cavity; the lower part of the first reaction tube is a tip part extending towards the direction of the thin wall, and external force is applied to shorten the relative distance between the tip part and the thin wall and puncture the thin wall.
2. The pretreatment reaction device according to claim 1, further comprising a sample discharge mechanism comprising a cylindrical sample discharge cylinder, a sample discharge port; the inner diameter of the upper part of the sample outlet cylinder is slightly larger than the outer diameter of the lower part of the second reaction tube, the sample outlet cylinder is inserted into the second reaction tube and is in sealing connection with the second reaction tube, and the lower part of the sample outlet cylinder is provided with a sample outlet; and the lower part of the second reaction tube is provided with a hole, and external force is applied to shorten the relative distance between the second reaction tube and the sample outlet so as to extrude the content in the second reaction tube.
3. The pretreatment reaction device according to claim 2, wherein the sample outlet mechanism further comprises a quantitative plug which is located inside the sample outlet cylinder and directly below the hole, is non-hermetically and fixedly connected with the sample outlet cylinder, and forms a sample outlet channel with the sample outlet, and the upper portion of the quantitative plug further comprises a sealing member extending to the outside of the quantitative plug body, the outer diameter of the upper portion of the quantitative plug is larger than the hole diameter of the hole, and the outer diameter of the lower portion of the quantitative plug is smaller than the hole diameter of the hole; the sealing element is positioned in the second reaction tube, so that the second reaction tube in the initial position forms a sealed cavity.
4. The pretreatment reaction device according to claim 3, wherein the quantitative plug is in threaded connection with the sample outlet cylinder, and a channel communicated with the sample outlet is arranged on the outer side of the plug body; the sealing element is in a cap shape and is integrally formed with the quantitative plug body.
5. The pretreatment reaction device as claimed in any one of claims 1 to 4, wherein an upper portion of said first reaction tube is engaged with said tip member to be movably connected thereto.
6. The pretreatment reactor apparatus according to claim 5, wherein said second reaction tube has a screw-threaded tube at an upper portion thereof and a protective tube at a lower portion thereof; the upper part of the screwing pipe is movably connected with the upper part of the first reaction pipe in an inserting and embedding manner, the middle part of the screwing pipe is provided with external threads, the lower part of the screwing pipe is provided with internal threads, and the lower pipe orifice of the screwing pipe is provided with the thin wall; the middle part of the tip part is provided with an external thread matched with the internal thread of the screwed pipe; the protective tube is cylindrical, the upper portion of the protective tube is provided with an internal thread matched with the external thread of the screwing tube, and the outer portion of the upper tube wall of the protective tube is fixedly connected with a disposable fracture limiting ring.
7. The pretreatment reaction device of any one of claims 2 to 4, wherein the outlet mechanism further comprises an outlet protective sheath.
8. The pretreatment reaction device of claim 1, wherein the first reaction tube and the second reaction tube are pre-loaded with a reagent.
9. The pretreatment reactor according to claim 1, wherein said tip member is a tube provided at a lower end thereof with a cutting edge or a spike.
10. The pretreatment reactor apparatus as set forth in claim 6, wherein said one-time fracture limiting ring is fixedly connected to the outer portion of the upper end tube wall of said protective tube at multiple points.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201921812473.6U CN210584971U (en) | 2019-10-29 | 2019-10-29 | Pretreatment reaction device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201921812473.6U CN210584971U (en) | 2019-10-29 | 2019-10-29 | Pretreatment reaction device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN210584971U true CN210584971U (en) | 2020-05-22 |
Family
ID=70700383
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201921812473.6U Active CN210584971U (en) | 2019-10-29 | 2019-10-29 | Pretreatment reaction device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN210584971U (en) |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111929105A (en) * | 2020-08-17 | 2020-11-13 | 珠海市迪奇孚瑞生物科技有限公司 | Integrated sampling device |
| CN112393960A (en) * | 2020-11-06 | 2021-02-23 | 奥然生物科技（上海）有限公司 | Sample pretreatment device and sample pretreatment method using same |
| CN114058472A (en) * | 2020-07-30 | 2022-02-18 | 苏州中加康美科技有限公司 | Nucleic acid reagent reaction unit |
| CN115322889A (en) * | 2022-10-17 | 2022-11-11 | 南京迪飞医疗器械有限公司 | Multi-section solvent reaction tube for biological nucleic acid detection and detection method thereof |
| CN118416979A (en) * | 2024-07-04 | 2024-08-02 | 湖南融健生物科技有限公司 | Sample adding device and sample adding method for microfluidic chip |
-
2019
- 2019-10-29 CN CN201921812473.6U patent/CN210584971U/en active Active
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114058472A (en) * | 2020-07-30 | 2022-02-18 | 苏州中加康美科技有限公司 | Nucleic acid reagent reaction unit |
| CN111929105A (en) * | 2020-08-17 | 2020-11-13 | 珠海市迪奇孚瑞生物科技有限公司 | Integrated sampling device |
| CN112393960A (en) * | 2020-11-06 | 2021-02-23 | 奥然生物科技（上海）有限公司 | Sample pretreatment device and sample pretreatment method using same |
| CN115322889A (en) * | 2022-10-17 | 2022-11-11 | 南京迪飞医疗器械有限公司 | Multi-section solvent reaction tube for biological nucleic acid detection and detection method thereof |
| CN118416979A (en) * | 2024-07-04 | 2024-08-02 | 湖南融健生物科技有限公司 | Sample adding device and sample adding method for microfluidic chip |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN210584971U (en) | Pretreatment reaction device | |
| WO2006121789A8 (en) | Telescoping closed-tube sampling assembly | |
| US20230404544A1 (en) | Self-contained sampling device for processing whole blood | |
| CN205209809U (en) | Trace liquid sampling device application of sample device and blood sampling external member thereof | |
| CN104116513A (en) | Self-priming type quantitative micro-quantity blood collection tube | |
| CN206208595U (en) | The sampling sample adding device of micro liquid | |
| CN105214745B (en) | A kind of micro-fluidic chip | |
| CN112940922A (en) | Full-integrated pathogen nucleic acid detection micro-fluidic chip | |
| CN211133966U (en) | Pretreatment reaction device | |
| CN206026361U (en) | Integrative device of disposable secretion sampling | |
| US6200781B1 (en) | Apparatus, system and method for automated execution and analysis of biological and chemical reactions | |
| CN205361370U (en) | Micro -fluidic chip | |
| CN207756167U (en) | A kind of filtering type liquid-transfering gun pipette tips | |
| CN205856451U (en) | A kind of sample processing device | |
| CN107828639B (en) | Sample injector with pretreatment function and sample pretreatment and sample injection method | |
| CN216513849U (en) | Closed nucleic acid detection device | |
| CN215506829U (en) | Rapid detection reaction reagent tube | |
| CN204855198U (en) | Quantitative sample thief of liquid sample trace | |
| CN204924717U (en) | Sample test device and sampler thereof | |
| CN107629939B (en) | Sample processing device | |
| CN220520497U (en) | Disposable sample extraction nucleic acid amplification detection device | |
| US7574931B2 (en) | Quantitative sampling device | |
| CN2809645Y (en) | Sample collection & assaying apparatus | |
| WO2023178711A1 (en) | Single-part rapid pcr chip | |
| CN219015677U (en) | Excrement sampling and excrement detection integrated device |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant |