CN107937263A - Self-check system and method based on gene magnification - Google Patents
Self-check system and method based on gene magnification Download PDFInfo
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Abstract
The present invention provides a kind of self-check system based on gene magnification.Including：One, from sampling apparatus, genetic test sample is voluntarily extracted with for subject；One gene detection reagent component, to carry out the container assemblies of sample process, sample transfer and gene amplification reaction as genetic test sample；One gene-amplificative instrament, to provide the reaction condition of sample process and gene amplification reaction and export a recognizable reaction result information；One mobile client, to store an Operating Guideline program, send foregoing recognizable reaction result information to a server end, and receives the sentence read result for can recognize that reaction result information of the server end feedback.For it is a set of can be independent of the gene self-check system of specialized laboratory.The system can be had quality control, accurate testing result can be obtained in the short time by personal user's Self-operating, step easy easily grasp, the portable easy to operate, inspection result of instrument.
Description
Technical field
The present invention relates to genetic test field, and in particular to a kind of self-check system and method based on gene magnification.
Background technology
Genetic test is detected, is analyzed using science of heredity, Protocols in Molecular Biology, so as to find specific hereditary thing
The process of matter exception.Protocols in Molecular Biology high speed development in the late three decades, genetic test is in clinical examination, precisely doctor
Treatment, Food Inspection quarantine, species discriminating etc. are widely used.
Usual genetic test based on the gene amplifications such as PCR, including PCR- fluorescence probe methods, PCR- hybrid methods,
Gene chips etc..It is upper generally by gene detection reagent manufacturer offer detection kit in application, examined by medical institutions
The detection kit that the professional institutions such as section, Food Inspection quarantine departments are provided using producer performs the detection to measuring samples, machine
Detection structure is fed back to the user for submitting inspection request by structure again.To examine Customer Acquisition inspection result as terminal, it is necessary to by
Producer provides reagent, inspection body and performs inspection, inspection body to user feedback inspection result, totally three links.It is wherein most heavy
The test stage wanted needs to rely on quality controlled gene magnification laboratory, the special instrument in laboratory and the experiment undergone training
The professional operation of member, generally requires longer round of visits, along with the report of providing of inspection body is also required to certain cycle,
So that the overall cycle that user obtains inspection result is longer.
By taking clinical examination as an example, whole gene detection process is as follows：
Diagnostic kit manufacturer supplies diagnostic kit to medical institutions；
User applies examining to the clinical department of healthcare structure, and clinical sample is delivered to and examines section office；
Examine section office to treat sample originally to test；
Then inspection result is being uploaded to the reporting system of medical institutions, survey report is submitted into user.
Whole process needs 5 cycles more than working day in general medical institutions.Wherein test stage, be limited to
The professional degree of operating method and instrument based on PCR, it is when needing 2-7 small that professional, which performs checkout procedure,.Therefore, when
The report period of preceding genetic test one week is universal phenomenon.In addition this typical checkout procedure, user are needed when applying examining
During collecting sample, obtain at least 2 times, relatively time-consuming effort live to medical institutions when reporting.
The content of the invention
In order to overcome dependence of the existing gene magnification testing process to specialized laboratory, special instrument, professional, inspection
Cycle length, the deficiency that detection process takes time and effort are surveyed, the present invention provides a kind of self-check system based on gene magnification.To be a set of
Be integrated with from sampling apparatus, detection kit, liquid relief instrument, gene-amplificative instrament it is portable, can be independent of Specialty Experiment
The gene self-check system of room.The system can be by personal user's Self-operating, the easy easily grasp of step, the portable easily behaviour of instrument
Make, inspection result has quality control, accurate testing result can be obtained in the short time.
The technical solution adopted in the present invention is：
A kind of self-check system based on gene magnification, including：
One, from sampling apparatus, genetic test sample is voluntarily extracted with for subject；
One gene detection reagent component, expands to carry out sample process, sample transfer and gene as genetic test sample
Increase the container assemblies of reaction；
One gene-amplificative instrament, it is recognizable anti-to provide the reaction condition of sample process and gene amplification reaction and export one
Answer result information；
One mobile client, is tied to store an Operating Guideline program, foregoing recognizable reaction is sent to a server end
Fruit information, and receive the sentence read result for can recognize that reaction result information of the server end feedback.
Further, the gene detection reagent component includes：The sample process container of built-in sample processing reagent, to
Carry out the liquid relief instrument of sample treatment solution transfer, the amplified reaction container of built-in amplification reaction reagent.
Further, the gene-amplificative instrament includes：
One sample process module, to carry out sample process to genetic test sample, and obtains sample treatment solution；
One amplified reaction module, to make sample treatment solution carry out amplified reaction in a reaction temperature section；
One control module, built-in control program are described to control program to control sample process module and amplified reaction
Module performs an operation for determining step.
Further, the sample process module obtains sample by being heated in a treatment temperature section to genetic test sample
Present treatment liquid, it includes：
To place the sample pool for the sample container for holding genetic test sample；
To heat the one of sample pool processing heating module；
To the cooling module to cool down to sample pool；
A processing sensing module of sample pool whether is put into judgement sample container；
To the treatment temperature sensing module of monitor sample pond temperature.
Further, the amplified reaction module includes：
To place the reaction tank of the reaction vessel for the container for holding sample treatment solution；
To heat the one of reaction tank reaction heating module；
To judge whether reaction vessel starts a reaction sensing module of amplified reaction；
To monitor the reaction temperature of reaction tank temperature sensing module.
Further, the gene-amplificative instrament further includes result visualization module, to by the result of amplified reaction with can
The mode intuitively read is presented；
Indicating module, to show that operating status and prompting operating personnel perform relevant operation；
The visualization model and indicating module synchronous signal link control module.
Further, type and detection target setting of the control program according to genetic test sample；The definite step
Suddenly include：
1) sample process module is preheated to heating-up temperature stabilization and is maintained at treatment temperature section, and indicating module prompting is put into base
Because detecting sample；
2) judge whether genetic test sample is put into, if so, then keeping heating-up temperature to be examined in treatment temperature section to gene
This continuous heating of test sample；
3) during heating time a to processing time, sample process module is to sample treatment solution cooling while amplified reaction module
Preheating；
4) when sample process module cools the temperature to a design temperature, sample treatment solution is taken out in indicating module prompting；
5) amplified reaction module is preheated to heating-up temperature stabilization and is maintained at reaction temperature section, and indicating module prompting is put into sample
Present treatment liquid；
6) whether judgement sample treatment fluid is put into, if so, then keeping heating-up temperature in treatment temperature section to genetic test
Sample continuous heating；
7) heating time to the amplified reaction time stops heating, and the result of amplified reaction is presented in visualization model.
Further, the treatment temperature section, reaction temperature section, processing time, the amplified reaction time is all in accordance with base
Because detecting type and the detection target setting of sample.
Present invention simultaneously provides a kind of self-sensing method based on gene magnification, including subject is according to a mobile client
Following steps are completed in the Operating Guideline program prompting of storage：
Voluntarily extract genetic test sample；
Genetic test sample is subjected to sample process, sample transfer and gene amplification reaction, obtains recognizable reaction result
Information；
Judge the recognizable reaction result information or send it to a server end then to receive the server end anti-
The sentence read result for can recognize that reaction result information of feedback.
A kind of Autonomous test server end based on gene magnification, including：
Receiving module, to receive the one of client transmission recognizable reaction result information；
Reading module, the recognizable reaction result information to interpretation, and sentence read result is fed back into the client.
By taking above-mentioned technical proposal, program and operating procedure are cured, carried out just for single kind sample
The genetic test of single odd number, improves convenience, reduces the difficulty of operation and professional degree.And coordinate mobile client
Instructed, the user for carrying out gene Autonomous test can be sampled with complete independently, sample process, gene magnification, result interpretation
Complete operation, aids in operating process without any instrument.The interior completion when 1.5 is small of whole operation process.The invention enables multiple
Miscellaneous genetic test operation can highly shortened acquisition detection by layman, the complete independently under non professional environment
As a result time, manpower and materials have been saved on the premise of testing result is ensured.Also so that in the general of non-genomic amplification laboratory
Genetic test is carried out under logical environment (such as clinical department, open air, home environment, basic hospital) to be possibly realized.
Brief description of the drawings
Fig. 1 to Figure 25 is aobvious by the mobile client in the self-check system based on gene magnification in one embodiment of the invention
The schematic diagram at the interface that the prompting user shown is operated.
Figure 26 is the module composition schematic diagram of gene-amplificative instrament in one embodiment of the invention.
Figure 27 is the module composition schematic diagram of sample process module in gene-amplificative instrament in one embodiment of the invention.
Figure 28 is the module composition schematic diagram of amplified reaction module in gene-amplificative instrament in one embodiment of the invention.
Figure 29 is the module composition schematic diagram of result visualization module in gene-amplificative instrament in one embodiment of the invention.
Figure 30 is the module composition schematic diagram of indicating module in gene-amplificative instrament in one embodiment of the invention.
Embodiment
Below in conjunction with the attached drawing in the embodiment of the present invention, the technical solution in the embodiment of the present invention is carried out clear, complete
Whole description.
In the embodiment of the application, there is provided a kind of self-check system based on gene magnification, including：
From sampling apparatus, genetic test sample is voluntarily extracted with for subject；
Gene detection reagent component, to carry out sample process, sample transfer and gene magnification as genetic test sample
The container assemblies of reaction；
Gene-amplificative instrament, to provide the reaction condition of sample process and gene amplification reaction and export a recognizable reaction
Result information；
Mobile client, to store an Operating Guideline program, send foregoing recognizable reaction result to a server end
Information, and receive the sentence read result for can recognize that reaction result information of the server end feedback.
Specifically, it is, for example, a kind of cell sample brush from sampling apparatus, can be with the help of without any auxiliary instrument
Smoothly complete cavity sampling, such as cervical cells sampling.
The general sampling swab clinically used is that supporting rod simple in structure adds swab structure, suitable for easily exposure
Sampling sites, and for the internal sampling sites similar to uterus neck, then it must could complete to adopt by expanding apparatus auxiliary
Sample.And the sample-taking brush in the present embodiment, using brush holder support plus the double-decker of brush pot, bristle is received in brush pot before sampling sites are reached
It is interior.Brush pot uses the smooth suitable supporting structure of outside diameter, can pass through cavity and reach sampling sites, its shoulder energy
Position-limiting action is enough played, after sampling sites are reached, brush head is released and carries out rotation sampling, brush head is withdrawn after sampling and is brushed
Cylinder.In the link of subsequent samples processing, the reed pinned on sampling brush body lifts brush holder support upwards, you can makes the brush head containing sample
Fall into the sample treatment solution of formulation.
Gene detection reagent component includes：The sample process container of built-in sample processing reagent, to carry out sample process
The liquid relief instrument of liquid transfer, the amplified reaction container of content amplification reaction reagent.
The way of realization of the gene detection reagent component is, for example, the cdna amplification kit according to detection drone design.Should
Kit is mainly comprising two kinds of sample treatment solution, gene amplification reagent components.The function of sample treatment solution is at sample
Reason, extracts the DNA of sample and carries out preliminary purification to it.The function of amplifing reagent is that the sample DNA of extraction expand instead
Should.
Wherein, the reaction tube as amplified reaction container, pre-installs gene detection reagent.
Usually carry out sample process first in gene magnification operation, will be micro with professional pipettor after sample process
Quantitative sample liquid is pipetted in detection reagent reaction tube, completes the structure of reaction system, be placed on amplification instrument and expanded
Increase reaction.In the present embodiment, the tube cover part of sample treatment solution is embedded quantitative valve core structure, makes it have the work(of quantitative liquid-discharging
Energy.
And sample treatment solution tube cover top be provided with connection injection needle interface, so as to injection needle assembly connection, by
This can utilize the penetrability of injection needle to pierce through the reaction tube of soft materials, and quantitative liquid is injected amplification system.Grasped in sample-adding
When making, sample injector is inverted by user, and injection needle downward, holds sample injector tube cover, fully presses tube body, is often pressed 1 time and is just had
The liquid discharge of accurate quantitative analysis, requires operation simple and practicable.
The tube cover of reaction tube in the present embodiment is, for example, double-decker, and inner cover is soft rubber plug, and outer cover center is opened
Hole, exposes interior rubber plug, can so be easy to the sample injector of injection needle construction to pierce through rubber plug, be loaded.If it is related to same
Sample, multiple detection targets, it would be desirable to be repeatedly loaded, this sample-adding operation for exempting from open and close lid and traditional sample loading alternative
Compare, it is more convenient.In addition, the material of the inside rubber plug of reaction tube can be selected springs back material for height, it is, for example, butyl silica gel, notes
Penetrating the perforation of pin sample-adding can voluntarily close, and after sample-adding, can maintain the seal of reaction system, prevent outside contamination.
With reference to figure 26, gene-amplificative instrament then specifically includes：Sample process module, to make genetic test sample in a processing
Temperature range carries out sample process, and obtains sample treatment solution；Amplified reaction module, to make sample treatment solution in a reaction temperature
Spend section and carry out amplified reaction；Control module, it is built-in；One control program, it is described to control program to control sample process module
And amplified reaction module performs the operation for determining step；Result visualization module, to by the result of amplified reaction with can be directly perceived
The mode of reading is presented；Indicating module, to show that operating status and prompting operating personnel perform relevant operation.Visualization model
And indicating module synchronous signal link control module, controlled by control module and perform relevant operation.
Wherein, program is controlled according to the type of genetic test sample and detection target setting.
Determine that step includes：
1) sample process module is preheated to heating-up temperature stabilization and is maintained at treatment temperature section, and indicating module prompting is put into base
Because detecting sample.
After being first turned on the power switch of amplification instrument, control module first implements complete machine self-test (conventional program self-test), self-test
By rear, control sample process module is begun to warm up, while allows sample process indicator light to send out a warning (in representing that heating carries out),
When target temperature is heated to, the temperature sensor meeting Real-time Feedback observed temperature set up is to control module, control module
After judging its arrival control temperature upper limit, it can stop heating, temperature can slowly decline thereafter, and when sensor feedback, its temperature reaches
When controlling lowest temperature, control module is notified that module reheats thus by dynamic control, maintains sample process module
One stationary temperature (surrounding a baseline fluctuation up and down).After arrival target temperature stablizes a bit of time (1-2min),
Control module control sample process indicator light is changed into green flicker state, notifies operator sample container can be inserted into sample
Pond.Cdna sample and the sample process agent of collection are contained in sample container.
2) judge whether genetic test sample is put into, if so, then keeping heating-up temperature to be examined in treatment temperature section to gene
This continuous heating of test sample.
After sample container is inserted into sample pool, infrared switch can be triggered, the timing of control module meeting automated execution, temperature control, hold
It is continuous that genetic test sample is heated.
3) during heating time a to processing time (such as 10 minutes), sample process module cools down at the same time sample treatment solution
Amplified reaction module preheats.
After sample process, while starting cooling module to obtaining sample treatment solution cooling, amplified reaction module is opened
Begin to preheat, ibid, warm amplified reaction indicator light takes on a red color.
4) when sample process module cools the temperature to a design temperature, sample treatment solution is taken out in indicating module prompting.
When the temperature of sample pool drops to design temperature (safe temperature), stop cooling, sample process indicator light green light dodges
Bright, prompting can take out sample container.After sample container is taken out, sample process indicator light automatic distinguishing, stops prompting.
5) amplified reaction module is preheated to heating-up temperature stabilization and is maintained at reaction temperature section, and indicating module prompting is put into sample
Present treatment liquid.
At this time, the sample treatment solution that user is manually operated in sample container is transferred to reaction vessel.Reaction vessel is generally
It is multiple.At this time, reaction tank is preheating to up to after target temperature, and observed temperature is fed back to control mould by the temperature sensor of reaction tank
Block, makes it stop heating, dynamic start-stop is so that it maintains target temperature (temperature control process of the process with sample process).In target
After temperature stabilization 1-2Min, the meeting green light flicker of amplified reaction indicator light, prompts operator to add reaction vessel.
6) whether judgement sample treatment fluid is put into, if so, then keeping heating-up temperature in treatment temperature section to genetic test
Sample continuous heating.
After reaction vessel is added, capping pond, while can trigger Hall switch immediately, starts timing, temperature control (reaction
Program), while amplified reaction indicator light is changed into red, expression is being heated.
7) heating time to the amplified reaction time (such as 40 minutes) stops heating, and amplified reaction is presented in visualization model
Result.
After the completion of response procedures, control module is automatically stopped reaction module heating, while orders unlatching light source, such as purple
The sample treatment solution of completion amplified reaction in outer light irradiation reaction vessel, while result prompting indicator light flicker, prompting can be with
Observe result.After observation, turn off the power switch.
Temperature described in the above process not refers in particular to an accurate point value or industry personnel's common cognition
With the temperature range for allowing to float and there is certain error of receiving.Above-mentioned treatment temperature section, reaction temperature section, processing
Time, type and detection goal-setting of the amplified reaction time all in accordance with genetic test sample.For example, it is directed to cervical cell DNA
Sample, it is room temperature to 100 DEG C (are, for example, 90 DEG C) that can preset treatment temperature, and predeterminable amplified reaction temperature is 65 DEG C ± 5 DEG C
In the range of a certain temperature.Temperature and time condition is preset all in accordance with applicable sample optimum reaction condition, for
Specially, single pattern detection, the setting of any program parameter need not be carried out when user is operated, it is only necessary to according to use
The prompting of explanation carries out simple operations.Operation instruction is write generally according to above-mentioned definite step, is pass on to user as referring to
Lead specification.
The concrete function framework of each group into module is described as follows below：
As shown in figure 27, sample process module includes：Place the sample pool for the sample container for holding genetic test sample；With
To heat the one of sample pool processing heating module；To the cooling module to cool down to sample pool；Whether judgement sample container is put into
The processing sensing module of sample pool；To the treatment temperature sensing module of monitor sample pond temperature, the example according to aforementioned process
Such as it is a temperature sensor.It is, for example, infrared sensor to handle sensing module.After sample container is put into sample pool, infrared biography is triggered
Sensor, infrared sensor send trigger signal and sample are heated to control module, control module control process heating module.It is infrared
Sensor model number is for example optional：Infrared transmitting tube model：IR204-A, reception pipe PT204-6B.
Need to comparative illustration be the amplification instrument that existing laboratory uses, such as PCR instrument is most of not have gene
Sample process function, although its correlation module can realize alternating temperature, but if to carry out sample process, it is necessary to temperature tune
Required temperature is made into, it is necessary to the operation of the knowledge and specialty of specialty, and the container of general sample process and amplified reaction
The size of container also differs, and the sample cell of PCR instrument is smaller, can not be adapted to the container for placing sample process.
As shown in figure 28, amplified reaction module includes：Place the reaction of the reaction vessel for the container for holding sample treatment solution
Pond；To heat the reaction heating module of reaction tank；The reaction temperature sensing module of reaction tank temperature is monitored, is, for example, a temperature
Sensor.Judge whether reaction vessel starts the reaction sensing module of amplified reaction, reaction sensing module be, for example, trigger-type suddenly
Your sensor, identification sample process liquid container are put into reaction tank, and reaction tank closing meet reaction heating condition send it is tactile
Signal to control module, control module control heating module heating.For example optional model of Hall sensor：UHE4913G-
AE3-R substitutes model：OH34N.
Specifically, reaction tank has multiple accommodating chambers, to accommodate multiple containers for holding sample treatment solution at the same time.
As shown in figure 29, result visualization module includes：To irradiate the light source for the sample treatment solution for completing amplified reaction；
To observe the observation window of light source irradiation result.
Amplified reaction inside modules are provided with the size fit light source that standing wave is grown really, such as ultraviolet source, optical source wavelength
Fluorescent dye in corresponding amplification reaction reagent.For example, corresponding a certain fluorescent dye chooses the light source of 365nm wavelength, see
Examining the position of opening of form can be such that the position of reaction solution in reaction vessel fully exposes, and the reaction system of positive amplification exists
Aobvious yellow-green fluorescence under the excitation of 365nm ultraviolet (bottom excitation), negative reaction also have weak yellow-green fluorescence.In order to make visually
The aberration of yin and yang attribute becomes apparent from, and considers to add one layer of colour filter before form is reacted, by other jammr band mistakes outside yellow green
Filter, become apparent from yellow green.So that the identification higher of reaction result yin and yang attribute color.
As shown in figure 30, indicating module includes：Sample process indicator light, to show sample process module heated condition and
Prompting adds genetic test sample；Amplified reaction indicator light, to show that amplified reaction module heated condition and prompting add sample
Present treatment liquid；As a result indicator light is prompted, to prompt observation testing result.
Using PCR instrument as representative, its Core Feature is to provide needed for gene magnification the gene-amplificative instrament of existing market mainstream
Accurate alternating temperature control program, instrument temperature-controlled precision is high, volume and weight is not easily shifted greatly, operation is relatively cumbersome, has professional requirement,
Need to set response procedures and reaction condition according to detection architecture.And general PCR instrument only has PCR modules, sample process needs it
He supports instrument.In contrast, the gene-amplificative instrament in the present embodiment, have cured one group of response procedures, to suitable for the condition
Amplification system be applicable in.Temperature control module includes two modules of sample process and amplified reaction.Whole operation process only needs a key
Start, you can order completes sample process and amplified reaction, as a result presents immediately.
The Core Feature of amplification instrument is to provide definite temperature-controlled conditions for sample process and amplified reaction.A key when in use
Start, when sample treatment solution sample injector is inserted into corresponding sample treatment solution sample cell, amplification instrument can be detected through sensor
To the addition of sample, operation temperature control program is automatically begun to, carrying out sample-adding by operator after sample process operates, by sample
DNA liquid adds target detection reagent reaction tube one by one, and when sample pool of the operator by reaction tube intercalation reaction area, amplification instrument can
The addition of reaction tube, automatic start gene magnification temperature control program are detected through default sensor.After amplification, reaction module
The light source of bottom automatically turns on, and the result of reaction tube is clear and legible under light source activation.User can pass through augmentor front portion
The direct observing response of observation window is as a result, judge yin and yang attribute., can be through foregoing mobile visitor if user judges to have any problem to color
APP interfaces calling mobile phone camera built in the end of family takes pictures reaction result, APP backstages, i.e. server end after upload pictures
Analysis calculating can be carried out to the particular optical data of photo, result is judged according to preset decision content, inspection is presented immediately
Survey result.Operation requirement of the whole process to instrument only has the start of one key of a step.
As it was previously stated, by increasing mobile client, using intelligent terminal auxiliary interpretation result and realize for operation
" foolproof guidance ", the app that can be mainly run in intelligent terminal depended on.
Referring to figs. 1 to Figure 25, the guide page that the APP interfaces according to mobile client installation are progressively shown is to a whole set of detection system
System is operated, and main flow is as follows：
1) user's use is completed from sampling (referring to figs. 1 to Fig. 6) from sampling apparatus；
2) the sample injector outer cover that sample treatment solution has been pre-installed in kit is opened, the brush head of sample-taking brush is fallen into sample
In treatment fluid, close outer cover (with reference to figure 7 to Figure 10)；
3) sample treatment solution sample injector is placed on amplification instrument, key start, automatically begins to sample process heating (reference chart
11 to Figure 12)；
It is to be appreciated that according to the guide page of diagram, it is first start preheating, is then sampled again, in fact, preheats and adopting
Sample is two independent operations, can sequentially adjust exchange as needed.Machine preheating is first opened, the time of preheating can be utilized
Go to sample, can so save the time.
The time-temperature of preheating, is all by programme controlled.The purpose of preheating is to reach target temperature, be exactly in fact
Heating, the not preheating on conventional meaning.
4) after sample process, the reaction tube of the injection needle-penetration prepackage detection reagent of sample injector front end will be quantitative
(with reference to figures 13 to 18) in liquid injection detection reagent；
5) by the sample cell of the reaction zone of reaction tube insertion amplification instrument, amplification instrument, which automatically detects sample and adds, starts timing
Temperature control is reacted (referring to figures 19 through 20)；
6) after reaction, the light source of instrument bottom automatically turns on, and reaction result can pass through the observation window of instrument front portion
Observation, yin and yang attribute can directly judge (with reference to figures 21 to 25) according to color.
As described above, whole operation process is by corresponding mobile client, such as the APP installed in mobile phone does operation and draws
Lead, user can complete to detect according to guiding step-by-step operation, confirmation.Testing result naked eyes can differentiate yin and yang attribute.
Due to adding fluorescent dye in reaction system, if reaction result is positive, yellow-green fluorescence appearance is just had.
Relative theory system known technology, details are not described herein.
If the user having any problem for color interpretation, it can be taken pictures with APP to reaction result, APP will pass through backstage
Calculate feedback sentence read result.Whole detection process only needs 1 detection suit (3 component), 1 mobile phone in the present embodiment, and 1.5 is small
When can obtain testing result.
APP realizes two functions：
1) user's operation guides, and guides client to be gradually completing operation in the form of picture and text；Guide the whole base of user's correct operation
Gene-amplification detects, and is the brief guiding training of user's operation, the process of user's operation according to guide page content step-by-step operation simultaneously
Confirm, enter lower one page；
2) result interpretation.The final step of operation guide is to allow user to open mobile phone camera to take pictures to reaction result, is uploaded
App can analyze image, be calculated afterwards, provide the final result of this detection.When result interpretation link is arrived in operation, if
Judgement of the user with the naked eye to reaction result color is had any problem, portion that can be by APP calling mobile phones camera to amplification instrument form
Divide and take pictures, image is analyzed by APP backstages after photo upload, is sentenced according to preset criterion to user feedback
Read result.
The detailed process of interpretation, is exactly the photo formed to mobile phone photograph, the color of the reagent of reaction tube is extracted,
Assignment, judgement.Only by current common colors recognizer i.e. can be achieved, each embodiment can use the existing prior art or
The main points of ripe program, not the application protection, those skilled in the art on the basis of foregoing disclosure is understood, with reference to
Known technology, it is applicable program that can select naturally, and details are not described herein.
In conclusion above-described embodiment described self-check system and method based on gene magnification improve gene certainly
The convenient degree of detection, reduces operation difficulty.The genetic test of more targets is carried out just for single kind sample.Determining inspection
After surveying target, it is possible to which for detection drone design detection reagent, and hardware components are such as amplification instrument, then generally applicable.Such as
System in previous embodiment is to detect the HPV viruse of 7 types.If in a further embodiment, it is necessary to detect 7 kinds
Influenza virus, or 7 kinds of hepatitis Bs, can equally use said system.Detection reagent is redesigned, adjusts the core of instrument
Parameter.
Obviously, described embodiment is only part of the embodiment of the present invention, instead of all the embodiments.Based on this hair
Embodiment in bright, the every other implementation that those of ordinary skill in the art are obtained without making creative work
Example, belongs to the scope of protection of the invention.
Claims (10)
- A kind of 1. self-check system based on gene magnification, it is characterised in that including：One, from sampling apparatus, genetic test sample is voluntarily extracted with for subject；One gene detection reagent component, it is anti-to carry out sample process, sample transfer and gene magnification as genetic test sample The container assemblies answered；One gene-amplificative instrament, to provide the reaction condition of sample process and gene amplification reaction and export a recognizable reaction knot Fruit information；One mobile client, to store and perform an Operating Guideline program, send foregoing recognizable reaction to a server end Result information, and receive the sentence read result for can recognize that reaction result information of the server end feedback.
- 2. the self-check system based on gene magnification as claimed in claim 1, it is characterised in that the gene detection reagent group Part includes：The sample process container of built-in sample processing reagent, to carry out the liquid relief instrument of sample treatment solution transfer, built-in expansion Increase the amplified reaction container of reaction reagent.
- 3. the self-check system based on gene magnification as claimed in claim 1, it is characterised in that the gene-amplificative instrament bag Include：One sample process module, to carry out sample process to genetic test sample, and obtains sample treatment solution；One amplified reaction module, to make sample treatment solution carry out amplified reaction in a reaction temperature section；One control module, built-in control program are described to control program to control sample process module and amplified reaction module Perform an operation for determining step.
- 4. the self-check system based on gene magnification as claimed in claim 3, it is characterised in that the sample process module is led to Cross to heat genetic test sample in a treatment temperature section and obtain sample treatment solution, it includes：To place the sample pool for the sample container for holding genetic test sample；To heat the one of sample pool processing heating module；To the cooling module to cool down to sample pool；A processing sensing module of sample pool whether is put into judgement sample container；To the treatment temperature sensing module of monitor sample pond temperature.
- 5. the self-check system based on gene magnification as claimed in claim 3, it is characterised in that the amplified reaction module bag Include：To place the reaction tank of the reaction vessel for the container for holding sample treatment solution；To heat the one of reaction tank reaction heating module；To judge whether reaction vessel starts a reaction sensing module of amplified reaction；To monitor the reaction temperature of reaction tank temperature sensing module.
- 6. the self-check system based on gene magnification as claimed in claim 3, it is characterised in that the gene-amplificative instrament also wraps Result visualization module is included, the result of amplified reaction to be presented in a manner of it can intuitively read；Indicating module, to show that operating status and prompting operating personnel perform relevant operation；The visualization model and indicating module synchronous signal link control module.
- 7. the self-check system based on gene magnification as claimed in claim 3, it is characterised in that the control program is according to base Because detecting type and the detection target setting of sample；The definite step includes：1) sample process module is preheated to heating-up temperature stabilization and is maintained at treatment temperature section, and indicating module prompting is put into gene inspection Test sample sheet；2) judge whether genetic test sample is put into, if so, then keeping heating-up temperature in treatment temperature section to genetic test sample This continuous heating；3) during heating time a to processing time, amplified reaction module is pre- at the same time to sample treatment solution cooling for sample process module Heat；4) when sample process module cools the temperature to a design temperature, sample treatment solution is taken out in indicating module prompting；5) amplified reaction module is preheated to heating-up temperature stabilization and is maintained at reaction temperature section, and indicating module prompting is put at sample Manage liquid；6) whether judgement sample treatment fluid is put into, if so, then keeping heating-up temperature in treatment temperature section to genetic test sample Continuous heating；7) heating time to the amplified reaction time stops heating, and the result of amplified reaction is presented in visualization model.
- 8. the self-check system based on gene magnification as claimed in claim 7, it is characterised in that the treatment temperature section, Reaction temperature section, processing time, type and detection target setting of the amplified reaction time all in accordance with genetic test sample.
- 9. a kind of self-sensing method based on gene magnification, including the Operating Guideline journey that subject stores according to a mobile client Following steps are completed in sequence prompting：Voluntarily extract genetic test sample；Genetic test sample is subjected to sample process, sample transfer and gene amplification reaction, obtains recognizable reaction result information；Judge that this can recognize that reaction result information or sends it to a server end and then receive the server end feedback Sentence read result for can recognize that reaction result information.
- 10. a kind of server end of the self-check system based on gene magnification, including：Receiving module, to receive the one of client transmission recognizable reaction result information；Reading module, the recognizable reaction result information to interpretation, and sentence read result is fed back into the client.
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