WO2019009663A1 - Composition destinée à prévenir et à atténuer le pied d'athlète - Google Patents

Composition destinée à prévenir et à atténuer le pied d'athlète Download PDF

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WO2019009663A1
WO2019009663A1 PCT/KR2018/007695 KR2018007695W WO2019009663A1 WO 2019009663 A1 WO2019009663 A1 WO 2019009663A1 KR 2018007695 W KR2018007695 W KR 2018007695W WO 2019009663 A1 WO2019009663 A1 WO 2019009663A1
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weight
composition
extract
athlete
foot
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PCT/KR2018/007695
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Korean (ko)
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최병규
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최병규
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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/61—Myrtaceae (Myrtle family), e.g. teatree or eucalyptus
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/045—Hydroxy compounds, e.g. alcohols; Salts thereof, e.g. alcoholates
    • A61K31/05—Phenols
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/33—Heterocyclic compounds
    • A61K31/335—Heterocyclic compounds having oxygen as the only ring hetero atom, e.g. fungichromin
    • A61K31/35—Heterocyclic compounds having oxygen as the only ring hetero atom, e.g. fungichromin having six-membered rings with one oxygen as the only ring hetero atom
    • A61K31/352—Heterocyclic compounds having oxygen as the only ring hetero atom, e.g. fungichromin having six-membered rings with one oxygen as the only ring hetero atom condensed with carbocyclic rings, e.g. methantheline 
    • A61K31/353—3,4-Dihydrobenzopyrans, e.g. chroman, catechin
    • A61K31/355—Tocopherols, e.g. vitamin E
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/13—Coniferophyta (gymnosperms)
    • A61K36/14—Cupressaceae (Cypress family), e.g. juniper or cypress
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/23—Apiaceae or Umbelliferae (Carrot family), e.g. dill, chervil, coriander or cumin
    • A61K36/238—Saposhnikovia
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/54—Lauraceae (Laurel family), e.g. cinnamon or sassafras
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/70—Polygonaceae (Buckwheat family), e.g. spineflower or dock
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/88—Liliopsida (monocotyledons)
    • A61K36/899—Poaceae or Gramineae (Grass family), e.g. bamboo, corn or sugar cane
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
    • A61K2236/30—Extraction of the material
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2300/00—Mixtures or combinations of active ingredients, wherein at least one active ingredient is fully defined in groups A61K31/00 - A61K41/00

Definitions

  • the present invention relates to a composition for prevention and improvement of athlete's foot. More particularly, the present invention relates to a cosmetic or an external-use composition for prevention and improvement of athlete's foot having skin protection, antioxidant and antimicrobial action simultaneously with natural oil as a main ingredient.
  • the hormone abnormality due to excessive stress in modern society and sudden changes in the external environment may cause the abnormal keratinization of the stratum corneum to fall off due to atmospheric pollutants or ultraviolet rays, The skin tone becomes unstable and the skin becomes rough and dull.
  • Skin aging is caused by oxidative damage of intracellular substances by free radicals. That is, highly reactive free radicals, such as active radicals such as hydroxy radicals and superoxide radicals, oxidize the lipid components constituting the cell membrane to destroy the cell membrane, thereby oxidizing the protein and stopping the function of the nucleic acid, It destroys its components and causes cell death.
  • free radicals such as active radicals such as hydroxy radicals and superoxide radicals
  • Antioxidant cosmetics By inhibiting oxidative damage in vivo, aging of the skin can be prevented or reduced.
  • Antioxidant cosmetics inhibit the oxidative damage of skin, including the oxidation of fat components, to prevent or delay skin aging.
  • the production amount and the market growth rate of a multi-functional cosmetic having two or more functionalities are much higher than those of a single functional cosmetic product.
  • Korean Patent Registration No. 10-1117564 discloses that excessive salt and / or sugar; Water phase component; Anionic, amphoteric and nonionic surfactants; And a polyol, wherein the salt is supersaturated and dissolved in the cosmetic composition, and the supersaturated and dissolved salt is present in the form of recrystallized particles, the water component is purified water, and the anionic surfactant is ammonium laureth sulfate ,
  • the amphoteric surfactant is cocamidopropyl betaine, the nonionic surfactant is cocamide di-is, and the polyol is glycerin.
  • the patent is based on the finding that the patent is capable of obtaining exfoliation, skin amelioration, blood circulation promoting effect and formulation safety by recrystallized salt and / or sugar particles and of remaining in a saturated solution of salt and / Salt and / or sugar exhibits a moisturizing function. It contains anionic, amphoteric and nonionic surfactant, and is excellent in skin cleansing effect and rinsability. Also, it is excellent in ease of use in the form of a gel.
  • the function of the normal cells due to the sterilization action peculiar to the salt is deteriorated and the collagen is destroyed thereby causing excessive aging of the skin.
  • the inventors of the present invention have made intensive studies to develop a cosmetic or composition for prevention and improvement of athlete's foot which has skin protection, antioxidant and antimicrobial action simultaneously with natural products, and as a result, Of course, it has been confirmed that it has an antioxidative and antimicrobial action and completed the present invention.
  • a flavor enhancer which comprises 50 to 75% by weight of tea tree oil, 10 to 30% by weight of avocado oil, 3 to 15% Athlete's foot (50%), vitamin E acetate (0.5 to 2 wt%), polysorbate (20) 0.5 to 2 wt%, benzyl alcohol 0.01 to 0.05 wt% and thymol 0.01 to 0.05 wt% Or an external composition for improvement.
  • composition for prevention or improvement of athlete's foot is a cosmetic or pharmaceutical composition for prevention and improvement of athlete's foot since the composition for preventing or improving athlete's foot is natural oil as a main ingredient and exhibits skin protection, antioxidant and antibacterial action simultaneously as well as less side effects such as toxicity and skin irritation Can be preferably used.
  • Figure 1 is a graph showing DPPH free radical scavenging performance of the example compositions.
  • the present invention provides a method for producing an extract, comprising, in one aspect, 50 to 75 wt% of tea tree oil, 10 to 30 wt% of avocado oil, 3 to 15 wt% of windshield extract or cottonwood extract, 3 to 15 wt%
  • a thickening agent for athlete's foot comprising 0.5 to 2% by weight of vitamin E acetate, 0.5 to 2% by weight of vitamin E acetate, 0.5 to 2% by weight of polysorbate 20, 0.01 to 0.05% by weight of benzyl alcohol and 0.01 to 0.05% Prevention or amelioration.
  • Tea Tree Oil one of the main components of the composition of the present invention, is a plant native to Australia and belongs to the genus Myrtaceae. It contains particulate matter in the leaves. When the leaves are crushed, Lt; / RTI > is obtained.
  • the tea trioyl contained 2.5% of alpha-pinene, 9.1% of alpha-terpinene, 3.9% of para-cymene, 4.3% of 1.8-cineol, 24.6% of gamma-terpinene, 2.3% of alpha-terpineol, 42.1%, terpinolene 4.1%, and trace amounts of residual components.
  • the effect of antibacterial as well as antiinflammatory, analgesic and wound treatments is effective. Its action mechanism is not clear as its effect is similar to that of ginseng.
  • tea tree oil is preferably compressed or rectified using leaves.
  • avocado oil is a vegetable oil made by squeezing flesh and skin.
  • avocado is rich in vitamin E called tocopherol, which is one kind of fat-soluble vitamin. It removes active oxygen from our body and suppresses aging. And is known to have the effect of preventing heart disease and cancer.
  • the avocado oil is preferably used by pressing or rectifying the flesh of the flesh.
  • the windshield (Saposhnikoviae divaricata Schiskin) is a perennial herb that belongs to the mountain-type Umbelliferae (Umbelliferae) and is a perennial herb called jinhwa wind, mountain wind wind, screen wind herb, mountain windbreak tree, It is widely distributed in Korea, China, Vietnam and Mongolia. It is known that the main effects of windblown by oriental medicine are sweating, fever, analgesic, diuretic, paralysis, limb paralysis, shinhae, geumdam, toothache, neuralgia, rheumatism and so on.
  • Chamaecyparis obtusa (Chamaecyparis obtusa) is an evergreen arborescent tree, and it is also called ashiko, hinoki and cypress. It is 30 ⁇ 40m in height and 1 ⁇ 2m in width.
  • the bark is reddish brown, and small needle-shaped leaves are densely grown on branches. It is distributed in Japan and southern part of Korea. It contains ⁇ -cadinene, ⁇ -terpineol and borneol, and leaves and wood contain 1% essential oil. . It is used as a natural insecticide for the prevention of house dust mites causing allergic diseases such as atopy, asthma and rhinitis. It is recommended to use the leaves, stems, and branches extracted from the leek.
  • the windshield and the leathery extract may be used singly or in the form of a mixture.
  • Rhizoma Polygoni Cuspidati is a plant belonging to the family Polygonaceae and grows in mountainous areas and is distributed in Korea, Japan, Taiwan and China. In one room, the roots are called hojanggun, and they are used as relaxation, diuretic, and tongue economy, and as a sedative in the private sector. When it is young, the stem looks like a hoof, and it has the name Ho Chang Geun. Root can be obtained by extracting roots and rootstocks.
  • Sasae is a plant of various ages of paddy field and grows frequently in the southern part of Korea and the mountains of Jeju Island.
  • the stem is straight up to 1 ⁇ 2m in height, 3 ⁇ 6mm in diameter, and the bract covers the stem for 2 ⁇ 3 years.
  • the nodes are covered with inverted hair and white powder, but the leaves of the sheath-shaped leaves disappear after 4 years.
  • Leaves are long, oval-shaped, 10 ⁇ 25cm long, pointed like a tail or long as the tail. There are no hairs on both sides of the leaf, there are hairs on the base of the back side, there are saw teeth like thorn on the edge, and the sheath has hairs. It is known to reduce fever and poison, remove sputum, urinate well, and smooth metabolism. Sasa can be obtained by extracting leaves, stems and roots.
  • the vegetable oil containing the tea tree oil and the avocado oil may be obtained by squeezing a leaf, a fruit or a fruit into plants or by adding an extraction solvent to the raw material at a weight ratio of 1: 2 to 1:20, and adding the extract to a reflux condenser After cooling by heating distillation for 2 to 3 hours, the extracted extract can be separately filtered for 5-7 hours.
  • the crude oil may be purchased commercially and used.
  • Leaves, stems, branches and the like can be extracted by hot water extraction, cold-watering or warm-up extraction.
  • the extraction solvent is mixed with the extraction solvent at a weight ratio of 2 to 20 times, and the mixture is extracted at 70 to 125 ° C for 2 to 24 hours, filtered and concentrated to obtain an extract
  • the bamboo shoots, bamboo shoots, and windshield roots are thoroughly cleaned, dried, and then added with an extraction solvent 5 to 10 times the weight of the raw materials.
  • the resulting mixture is placed in a autoclave autoclave and heated at 65 to 125 ° C for 2 to 6 hours, Extraction by heating for 5 hours, filtration and concentration may be obtained
  • the extraction solvent may be at least one selected from water, lower alcohols having 1 to 4 carbon atoms, polyhydric alcohols, and mixtures thereof.
  • the lower alcohol having 1 to 4 carbon atoms methanol, ethanol and the like can be used.
  • the polyhydric alcohol butylene glycol, propylene glycol, pentylene glycol and the like can be used. Mixtures of water and lower alcohols, mixtures of water and polyhydric alcohols, mixtures of lower alcohols and polyhydric alcohols, or mixtures of water and lower alcohols and polyhydric alcohols can be used as the mixture.
  • the present invention is mainly characterized in finding ingredients having multifunctional properties such as skin protection, antioxidant activity and antibacterial property from natural plants and an optimal combination ratio thereof.
  • the tea tree oil, avocado oil, Extracts and vitamin E acetates are the main components of skin protection, antioxidant, and antibacterial activity.
  • Sasa extract acts as a preservative.
  • Polysorbate acts as a dispersant.
  • Benzyl alcohol and thymol act as preservatives and stabilizers. do.
  • composition ratio of the composition according to the present invention is set considering the effective dose and side effects of each component, and if the ratio is out of the range, the effect may be deteriorated.
  • the desired effects of the present invention can be achieved by mixing the above-mentioned natural plant extracts at an optimum ratio, thereby preventing growth of various microorganisms and maximizing cell protection and antioxidative effects.
  • the antibacterial effect is insufficient.
  • the amount of the tea trioyl is more than 75 wt%, the effect of increasing the amount of the tea trioyl is insufficient.
  • the antioxidative and preservative effect of avocado oil is less than 10% by weight, and when it is more than 30% by weight, the synergistic effect by combination with each ingredient may not be achieved.
  • the skin protection, antioxidant and germicidal effect are less than 3% by weight of the roots extract or the cotton bud extract and the silkworm extract, and less than 15% by weight are uneconomical because the composition is not balanced with the whole composition.
  • the content of the sage extract is 1 to 10% by weight, the content of vitamin E acetate is 0.5 to 2% by weight, the content of polysorbate 20 is 0.5 to 2% by weight, the content of benzyl alcohol is 0.01 to 0.05% by weight and the content of thymol is 0.01 to 0.05% %, It is possible to optimally exhibit stable coordination and various functions of the entire composition.
  • composition according to the present invention contains an extract of a natural substance, which is not a chemical synthesis agent, it can be seen from the results of Examples and Test Examples described later that there is no irritation or toxicity to the skin and thus it is excellent in stability. But also can be used as a cosmetic or therapeutic agent requiring such functionality since it has antimicrobial activity against bacteria including aceotrophic bacteria, healing of atopic dermatitis, therapeutic treatment of infant's dirt, protection against skin and improvement of skin texture have.
  • the present invention provides, in a further aspect, a composition for improving athlete's foot comprising the composition and cosmetics or a pharmaceutically acceptable carrier as an active ingredient.
  • the formulations of the cosmetics may be prepared by conventional methods well known in the art of cosmetics.
  • excipients of these cosmetics may vary depending on the purpose of use, but they generally contain a preservative such as glycerin, butylene glycol or propylene glycol or a vegetable preservative, a purified water, a moisturizer, a bulking agent, a cream agent, a surfactant, a pH adjuster, It is preferable to mix them according to the form of the desired formulation.
  • a preservative such as glycerin, butylene glycol or propylene glycol or a vegetable preservative
  • a purified water a moisturizer, a bulking agent, a cream agent, a surfactant, a pH adjuster, It is preferable to mix them according to the form of the desired formulation.
  • composition of the present invention can be suitably formulated in the form of an external preparation such as a suspension, emulsion or aerosol according to a conventional method.
  • composition of the present invention is prepared from a pharmaceutical composition, it may be preferable to further include a conventional pharmaceutically acceptable carrier.
  • the dosage of the pharmaceutical composition of the present invention is suitably set in accordance with the form of the preparation, the method of administration, the purpose of use, and the age, body weight, and symptoms of the patient to which the active ingredient is administered. For example, 10 mu g to 200 mg / kg per day for an adult.
  • the pharmacokinetic properties of a particular reagent and its mode and route of administration Age, health, weight; It will be understood that the dosage varies depending on known factors such as the nature and extent of the symptoms, the type of concurrent treatment, the frequency of treatment and the desired effect.
  • Each of the components obtained in Examples 1 to 3 was formulated in a formulation ratio shown in Table 1 below to prepare a composition for preventing athlete's foot.
  • Each plant oil and extract was mixed with purified water extract and alcohol extract at a weight ratio of 1: 1.
  • Example 4 Example 5
  • Example 6 Example 7
  • Example 8 Comparative Example Tea trio day 50 55 53 65 70 70
  • Avocado oil 10 30 10 10 20
  • 1.0 - Vitamin E-acetate 0.94 0.94 0.50 2.0 1.0 - Polysorbate (20) 2.0 0.50 0.90 0.9 0.9 - Benzyl alcohol 0.01 0.05 0.05 0.05 0.05 - Thymol 0.05 0.01 0.05 0.05 0.05 - Sum 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100
  • Test Example 1 Antioxidative effect analysis of natural extract composition
  • the in vitro antioxidant activity was measured by reacting the free radical DPPH having the maximum absorbance at 517 nm with the compositions of Examples 4 to 8 to determine the antioxidative effect using the absorbance value lowering in yellow.
  • DPPH free radical scavenging activity was determined using a reaction mixture comprising the composition of the example dissolved in methanol in a 96-well microplate and 0.1 mM DPPH dissolved in ethanol. The extracts were mixed with 0.6 mL of DPPH solution and incubated at room temperature for 20 minutes. The absorbance of the reaction mixture was then measured at 517 nm. The percent inhibition of radical scavenging was determined in comparison to the ethanol treated group or the ascorbic acid (100 [mu] g) treated group. The percentage of DPPH free radical scavenging activity was calculated by the following equation.
  • Figure 1 is a graph showing DPPH free radical scavenging performance of the example compositions.
  • Con vehicle treated group as a negative control group
  • AA treated with ascorbic acid (1 mg / ml) as a positive control group
  • Sample of 100 ⁇ sample (hereinafter, the same as in the test example).
  • Hydroxyl radical scavenging activity was measured by reacting hydroxyl radicals generated by Fenton ' s reagent with the composition of the examples to lower the absorbance value.
  • Embodiment the composition 100 ⁇ M EDTA, it was added to the reaction mixture containing oxy-ribose 1 mM H 2 O 2, and 2.8 mM to. The volume was supplemented with 20 mM pH 7.4 phosphate buffer to make 1 mL and then incubated at 37 ° C for 1 hour.
  • FIG. 2 is a graph showing the hydroxy radical scavenging activity of the example composition.
  • Positive control ascorbic acid (AA) inhibited free radicals by 51.69 ⁇ 1.13% at 1 mg / ml concentration.
  • the results are shown in Table 1.
  • the sample treatment groups were 41.25 ⁇ 0.67 (Example 4), 42.75 ⁇ 0.76 (Example 5), 47.87 ⁇ 0.83 (Example 6), 45.21 ⁇ 0.96 (Example 7), 39.01 ⁇ 0.89 23.50 ⁇ 1.23 (comparative) (FIG. 2).
  • compositions of the examples were mixed with 100 ⁇ L of Folin-Denis reagent and incubated for 3 minutes at room temperature (RT, 22-24 ° C.)
  • the reaction mixture was neutralized with 100 ⁇ l of 10% sodium carbonate and incubated for 1 hour.
  • Absorbance was measured at 760 nm with a plate reader. Polyphenol content was calculated using gallic acid as standard.
  • Example 4 Example 5
  • Example 6 Example 7
  • Example 8 Comparative Example Polyphenol (mg / g) 56.72 + 1.51 57.32 + - 0.98 52.77 + - 0.54 56.45 + 0.71 52.34 + - 0.35 25.43 + - 0.23
  • Flavonoid (mg / g) 7.41 ⁇ 0.82 8.55 0.35 8.01 + - 0.78 8.25 + - 0.66 7.15 ⁇ 0.78 2.16 ⁇ 0.35
  • Reducing power efficacy analysis was based on the comparison of the effect of ascorbic acid with the lowering of the absorbance value by reacting the composition of each Example with the induction of oxidative reaction by FeCl 3 .
  • compositions of the examples were mixed with 0.5 mL of 200 mM sodium phosphate buffer (pH 6.6), and 0.5 mL of 1% potassium ferricyanide, then the mixture was incubated at 50 DEG C for 20 minutes, followed by 0.5 mL of 10% TCA . It was centrifuged at 650 g for 10 minutes and 1 mL of supernatant was mixed with equal volume of distilled water and 0.2 mL of 0.1% ferric chloride. The same treatment was performed on standard ascorbic acid solution and the absorbance was measured at 700 nm. The reducing power was calculated and expressed in terms of ascorbic acid.
  • Example 4 Example 5
  • Example 6 Example 7
  • Example 8 Comparative Example The ascorbic acid equivalent (g) 28.12 ⁇ 1.51 27.23 + - 0.31 22.67 ⁇ 0.51 26.35 ⁇ 0.78 22.36 + - 0.92 13.34 ⁇ 0.53
  • the activity of Fe 2+ chelating activity was measured by reacting the composition of the present invention with the induction of the oxidative reaction by the Fe 2+ -ferrozine complex to lower the absorbance value.
  • Fe 2+ chelating ability (%) (Abs control without sample - Abs sample ) / Abs control without sample ⁇ 100.
  • the negative control group was treated with vehicle (Tris-HCl buffer) and the positive control group was treated with ascorbic acid to analyze Fe 2+ chelating activity.
  • FIG. 3 is a graph showing the results of Fe 2+ chelating assay analysis of the composition of the examples.
  • Positive control ascorbic acid (AA) inhibited the oxidative response of Fe 2+ -ferrozine complex by 39.08 ⁇ 3.92% at 1 mg / ml concentration.
  • the compositions of each example inhibited the oxidative response of the Fe 2+ -ferrozine complex by 46.21 ⁇ 1.02% to 53.25 ⁇ 2.02% at 100 ⁇ l, while suppressing the comparative example by 18.51 ⁇ 1.85%.
  • nitrite scavenging activity was measured by comparing the absorbance of the nitrite with the composition of the examples to determine the antioxidative effect.
  • vehicle (PBS) was treated with negative control group and nitrite scavenging ability was treated with ascorbic acid in positive control group.
  • Each sample was treated with 0.1 N HCl solution (pH 1.2), pH 3.0 and pH 5.0 solution, and 1 mM NaNO 2 solution was added. The mixture was incubated at 37 [deg.] C for 1 hour. 2% acetic acid and Griess reagent were added and incubated at room temperature for 15 minutes. The absorbance of the mixture was measured at 520 nm.
  • Nitrite inhibition (%) [1 - (Abs sample / Abs control without sample )] x 100.
  • Control group treated with PBS as a negative control group
  • AA a group treated with ascorbic acid (1 mg / ml) as a positive control
  • 100 ⁇ g 100 ⁇ g of a composition as a sample group.
  • Positive control ascorbic acid (AA) inhibited nitrite at a concentration of 1 mg / ml by 91.0 ⁇ 3.12% at pH 1.2.
  • the composition of each example inhibited nitrite at 56.73 ⁇ 1.12% (Example 4) to 61.32 ⁇ 1.09% (Example 7) at pH 1.2 and 20.51 ⁇ 0.76% inhibition of the comparative example.
  • compositions according to Examples 4 to 8 were tested by disc paper test.
  • test strain was dispensed into a petri dish, spread over the entire medium using a glass rod, and then placed on a paper disc having a diameter of 5 mm. Then, 30 ⁇ l of the composition of each example was placed on a paper disc. After overnight incubation at 30 ° C, the inhibition zone was checked for antimicrobial activity. The results are shown in Table 4 below.
  • the composition according to the present invention has excellent antimicrobial activity against bacteria such as fungi such as aphthous fungus, though it has a difference in degree. Therefore, it can be used as a cosmetic product or a treatment for athlete's foot.
  • Test Example 3 Measurement of growth inhibitory activity of fungi
  • the size of the growth inhibition ring by the composition of the examples was 2.5 cm or more and showed excellent fungicidal activity. These antimicrobial activities were similar in other fungi.
  • Skin irritation experiments were conducted to examine the degree of irritation of each composition according to the present invention to experimental animals.
  • Eighteen New Zealand white rabbits weighing 2.3 to 2.7 kg were used as experimental animals.
  • experimental animals were refined in an animal room for one week after their intake, and normal animals were observed during the refinement period and only healthy animals were used for the test.
  • the rabbits were divided into treatment compartments and control compartments 24 hours before application of the test substance, and then 0.5 ml of the test substance per test animal was applied once to the administration site, and sterilized physiological saline was applied to the untreated control compartment in the same amount . After attaching, it was covered with a sheet of solid material, fixed with a tape, and applied for a certain period of time.
  • the application portion was gently rinsed with physiological saline.
  • local anesthetics lidocaine, Gwangmyeong drug
  • lidocaine, Gwangmyeong drug were injected through the ear vein and euthanized, followed by autopsy. And observed with naked eyes.
  • compositions of the examples were subjected to acute toxicity studies and their toxicity was observed.
  • male and female SD rats of 105 weight and 105 weight, respectively were used and tested by using the compositions of the respective examples and distilled water as a negative control.
  • the rats were incubated in a laboratory incubation box with a temperature of 22 ° C, a relative humidity of 53% and a fluorescent light (09:00 lit-18: 00 off) contrast cycle and a light intensity of 150-300 Lux for about a week , And then healthy animals were selected and divided into groups so that the average body weight was matched.
  • the animals were orally administered at a dose of 20 ml / kg once a day for 14 days, and then the changes in general condition, poisoning symptoms, , Autonomic nerves, weight change, and presence of dead animals.
  • the present invention relates to a cosmetic or pharmaceutical composition for prevention and improvement of athlete's foot since the composition for prevention or improvement of athlete's foot is natural oil as a main component and exhibits skin protection, antioxidant and antimicrobial action simultaneously as well as less side effects such as toxicity and skin irritation It is useful in the production of related products.

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  • Microbiology (AREA)
  • Medical Informatics (AREA)
  • Botany (AREA)
  • Biotechnology (AREA)
  • Alternative & Traditional Medicine (AREA)
  • Medicines Containing Plant Substances (AREA)

Abstract

La présente invention concerne une composition destinée à prévenir et à atténuer le pied d'athlète. Plus précisément, la présente invention concerne une composition cosmétique ou pharmaceutique destinée à prévenir et à atténuer le pied d'athlète qui a simultanément une activité protectrice de la peau, antioxydante et antimicrobienne du fait qu'elle comprend une huile naturelle comme ingrédient principal. Puisque la composition destinée à prévenir ou à atténuer le pied d'athlète selon la présente invention comprend une huile naturelle comme ingrédient principal, et présente ainsi peu d'effets secondaires tels que la toxicité et l'irritation de la peau, et qu'elle présente simultanément une activité protectrice de la peau, antioxydante et antimicrobienne, ladite composition peut être utilisée de préférence comme composition cosmétique ou pharmaceutique destinée à la prévention et à l'atténuation du pied d'athlète.
PCT/KR2018/007695 2017-07-06 2018-07-06 Composition destinée à prévenir et à atténuer le pied d'athlète WO2019009663A1 (fr)

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KR1020170086138A KR101869763B1 (ko) 2017-07-06 2017-07-06 무좀 예방 및 개선용 조성물
KR10-2017-0086138 2017-07-06

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KR101869763B1 (ko) * 2017-07-06 2018-06-21 최병규 무좀 예방 및 개선용 조성물
KR20230114445A (ko) 2022-01-25 2023-08-01 이지영 무좀 예방 및 개선용 조성물

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KR20070079497A (ko) * 2006-02-02 2007-08-07 주식회사 에스티씨나라 생약성분 추출물을 함유하는 피부 외용제 조성물
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