WO2018230575A1 - Système de microscope - Google Patents

Système de microscope Download PDF

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Publication number
WO2018230575A1
WO2018230575A1 PCT/JP2018/022475 JP2018022475W WO2018230575A1 WO 2018230575 A1 WO2018230575 A1 WO 2018230575A1 JP 2018022475 W JP2018022475 W JP 2018022475W WO 2018230575 A1 WO2018230575 A1 WO 2018230575A1
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WIPO (PCT)
Prior art keywords
image
luminance
unit
microscope system
sample
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Application number
PCT/JP2018/022475
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English (en)
Japanese (ja)
Inventor
哲成 韓
広 渡邉
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オリンパス株式会社
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Publication of WO2018230575A1 publication Critical patent/WO2018230575A1/fr

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    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/06—Means for illuminating specimens
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/36—Microscopes arranged for photographic purposes or projection purposes or digital imaging or video purposes including associated control and data processing arrangements
    • H—ELECTRICITY
    • H04—ELECTRIC COMMUNICATION TECHNIQUE
    • H04N—PICTORIAL COMMUNICATION, e.g. TELEVISION
    • H04N23/00—Cameras or camera modules comprising electronic image sensors; Control thereof
    • H04N23/70—Circuitry for compensating brightness variation in the scene

Definitions

  • the present invention relates to a microscope system that observes a sample placed on a stage by illuminating it.
  • the present invention has been made in view of the above, and in a polygonal illumination having a plurality of illuminations, a microscope system capable of reducing reflection and reflection by equalizing the light amount of each illumination applied to the sample. Intended to be provided.
  • a microscope system has two or more segments emitting light in a microscope system for observing a sample, and changes the lighting site of the segments And an operation unit that receives settings of the illumination pattern, the light amount, and the lighting site of the polygonal illumination, the objective lens that condenses the observation image of the sample, and An imaging unit for imaging a sample; a luminance information extraction unit for extracting luminance information of a calibration image captured by the imaging unit in a state where the lighting site of the segment is changed; and a reference image in the calibration image A luminance average value comparison unit that compares luminance information with luminance information of another calibration image, and acquires luminance ratio information for each lighting portion of the segment , Based on the luminance ratio information, characterized in that it comprises a control unit for controlling the photographing conditions of the sample.
  • control unit includes a polygonal illumination control unit which adjusts the light amount of the segment based on the luminance ratio information.
  • control unit includes a camera control unit that adjusts the exposure time of the imaging unit according to the lighting part of the segment based on the luminance ratio information. It features.
  • the microscope system according to the present invention further includes a brightness average value calculating unit that calculates a brightness average value from the brightness information.
  • the luminance average value is compared with the luminance average value of another calibration image, and luminance ratio information is acquired for each lighting part of the segment.
  • an image storage unit storing the calibration image
  • a brightness information storage unit storing brightness information of the calibration image
  • brightness ratio information of the calibration image and a calibration information storage unit for storing
  • control unit may display the image data, and the control unit may control the imaging condition based on the luminance ratio information to be outside a predetermined threshold.
  • the display unit may display that the control of the photographing condition can not be performed.
  • the microscope system according to the present invention is characterized in that, in the above-mentioned invention, the microscope system further includes an image processing control unit that combines a plurality of image data of the sample acquired under different imaging conditions.
  • the image processing control section performs the image synthesis on the basis of the luminance standard selected from the plurality of luminance standards when synthesizing the plurality of image data. Do.
  • the microscope system according to the present invention is characterized in that, in the above-mentioned invention, the brightness reference has four or more stages.
  • the microscope system according to the present invention is characterized in that, in the above-mentioned invention, the automatic exposure of the target of the imaging section is set high to capture an image, and a plurality of obtained images are synthesized based on a low luminance standard. .
  • the position of the hole of the revolver mounted with the revolver for switchably inserting and removing the plurality of objective lenses on the optical path and the objective lens disposed on the optical path
  • the calibration information storage unit stores luminance ratio information for each hole position of the revolver.
  • the microscope system further includes an input unit for receiving input of various instructions, and the display unit is selected when hole position information of the revolver is selected through the input unit.
  • the image group photographed at the specified hole position is extracted and displayed, and when one image is selected from the image group displayed on the display unit, the lighting site of the polygonal illumination at the time of photographing the selected image is displayed It is characterized by
  • the microscope system further includes an input unit for receiving inputs of various instructions, and the display unit includes hole position information of the revolver and a lighting portion of the polygonal illumination is the input unit. When it is selected, it is characterized by extracting and displaying the image photographed at the selected hole position and lighting site.
  • a microscope system is a microscope system for observing a sample, having two or more segments emitting light, changing the lighting site of the segment to irradiate the sample with light of different irradiation patterns. Operation of the polygon illumination, the illumination pattern of the polygon illumination, the light amount, and the setting of the lighting site, the objective lens for condensing the observation image of the sample, the imaging unit for imaging the sample, and lighting of the segment.
  • the control unit that controls the imaging condition of the sample based on the information of the calibration image captured by the imaging unit in a state where the region is changed, and the plurality of samples acquired under the imaging condition based on the information of the calibration image
  • the image processing control unit is configured to combine image data.
  • the microscope system according to the present invention it is possible to obtain an image in which reflection and reflection are reduced by making uniform the light amount of each of the illuminations constituting the polygonal illumination to be applied to the sample.
  • FIG. 1 is a schematic view showing a schematic configuration of a microscope system according to a first embodiment of the present invention.
  • FIG. 2A is a diagram showing a lighting part of LED lighting at the time of lighting mode change of the polygon lighting in the microscope system according to the first embodiment.
  • FIG. 2B is a diagram showing a lighting site of LED illumination at the time of segment rotation of polygonal illumination in the microscope system according to the first embodiment.
  • FIG. 3 is a flow chart for explaining a process of photographing a sample in the microscope system according to the first embodiment of the present invention.
  • FIG. 4 is a flow chart for explaining the calibration image acquiring step of FIG.
  • FIG. 5 is a flow chart for explaining the luminance reference setting process of FIG. FIG.
  • FIG. 6 is a flowchart illustrating the brightness adjustment process of FIG.
  • FIG. 7 is a flowchart illustrating the imaging process of FIG.
  • FIG. 8 is a diagram showing an example of the image combining screen.
  • FIG. 9 is a schematic view showing a schematic configuration of a microscope system according to a second embodiment.
  • FIG. 10 is a flowchart for describing a process of photographing a sample in the microscope system according to the second embodiment of the present invention.
  • FIG. 11 is a diagram for explaining an image display screen displayed on the display unit in the microscope system according to the second embodiment of the present invention.
  • FIG. 12 is a flowchart of image preview display using the image display screen of FIG.
  • FIG. 13 is a flowchart of another form of image preview display using the image display screen of FIG.
  • FIG. 1 is a schematic view showing a schematic configuration of a microscope system according to a first embodiment of the present invention.
  • the microscope system 100 irradiates illumination light for bright field (BF) illumination onto the sample 2, the stage 3 on which the sample 2 is placed, the objective lens 4 disposed to face the stage 3, and the sample 2.
  • BF bright field
  • Coaxial epi-illumination light source 5 polygon illumination 6 for illuminating illumination light for dark field (DF) illumination on the sample 2, eyepiece 7 for observing light reflected from the sample 2, and imaging light reflected from the sample 2 While displaying the image corresponding to the image data which the camera 8 for imaging
  • the microscope main body unit 1, the imaging camera 8, the control unit 20, the image processing apparatus 30, the storage unit 40, and the operation unit 50 are connected by wire or wireless so that data can be transmitted and received.
  • the microscope body 1 has a substantially C-shape in a side view, supports the stage 3, and has a microscope frame 12 that holds the objective lens 4 via the revolver 11.
  • the stage 3 is configured to be movable in the XYZ axis directions, and moves in response to an operation of a stage operation unit (not shown).
  • the revolver 11 is provided slidably or rotatably with respect to the microscope frame 12, and the objective lens 4 is disposed above the sample 2.
  • the revolver 11 holds a plurality of objective lenses 4 having different magnifications (observation magnifications).
  • the objective lens 4 is mounted on the revolver 11.
  • the stage 3 is moved in the optical path direction (Z-axis direction), and focusing is adjusted.
  • the coaxial epi-illumination light source 5 is a lamp house 13 having an epi-illumination light source 13 a that emits illumination light for bright field illumination and a projection that condenses the illumination light emitted by the epi-illumination light source 13 a and emits it to a mirror 14 a And a light pipe 15.
  • a condenser lens 15a for condensing the illumination light for bright field illumination emitted by the incident light source 13a is disposed in the light projection tube 15.
  • the illumination light for bright field illumination emitted from the epi-illumination light source 13a is irradiated to the sample 2 through the illumination optical system such as the condenser lens 15a, the mirror 14a and the objective lens 4 and the reflected light from the sample 2 is the objective lens. 4, the mirror 14a, the imaging lens 17a in the trinocular tube 17, and the split prism and mirror (not shown) introduce it to the eyepiece lens 7 or the photographing camera 8 for visual observation and the like.
  • the polygonal illumination 6 a plurality of LED illuminations 61 disposed in a ring are disposed in a ring.
  • the illumination light for dark field illumination emitted from the LED illumination 61 is substantially parallel light, passes through a dark field optical path provided outside the optical path center of the objective lens 4, and is applied to the sample 2.
  • the polygonal illumination 6 includes 16 LED illuminations 61, and each of the LED illuminations 61 is divided into segments, and the polygonal illumination control unit 22 described later performs lighting and extinguishing for each segment. It is controlled.
  • FIG. 2A is a diagram showing a lighting part of the LED lighting 61 when the lighting segment of the polygonal lighting 6 is changed.
  • a black circle indicates the LED illumination 61 that lights up
  • a white circle indicates the LED illumination 61 that turns off.
  • a mode in which one LED illumination 61 is lit (FIG. 2A (1)), a mode in which the LED illumination 61 is illuminated by a quarter (FIG. 2A (2)), and the LED illumination 61 is lit by 1/2 Mode (FIG. 2A (3)) and a mode (FIG. 2A (4)) in which all the LED lights 61 are turned on.
  • the lighting mode ((1) to (4) in FIG. 2A) can be changed by the operation unit 50. By changing the lighting mode, observation of the unevenness and the like of the sample 2 becomes easy.
  • FIG. 2B is a diagram showing a lighting part of the LED illumination 61 at the time of segment rotation of the polygonal illumination 6.
  • FIG. 2B shows the lighting part of the LED lighting 61 when the segment to be lit rotates in a state where the pattern (FIG. 2A (2)) in which four (one-fourth) LED lightings 61 are lit is selected. There is.
  • rotating the knob of the rotation operation unit (not shown) of the operation unit 50 in a state (FIG. 2B (2)) in which the upper left four LED illuminations 61 arranged in a ring shape are turned on (FIG. 2B (2)) Can be rotated.
  • the rotation of the lighting portion of the LED lighting 61 facilitates observation of the unevenness and the like of the sample 2.
  • the imaging camera 8 converts light into an electrical signal (analog signal) by receiving an observation image (observation light) of the sample incident through the objective lens 4 and the imaging lens 17 a and performing photoelectric conversion.
  • Image processing device such as a CCD (Charge Coupled Device) or a Complementary Metal Oxide Semiconductor (CMOS) having a plurality of pixels, and an electrical signal output from the imaging device after signal processing such as amplification (gain adjustment)
  • a signal processing unit (not shown) that converts image data of a digital sample by performing / D conversion and outputs the image data to the image processing apparatus 30 is configured.
  • the imaging camera 8 continuously generates image data of a sample at minute time intervals and outputs the image data to the image processing apparatus 30 under the control of the camera control unit 21 described later. Further, the photographing camera 8 generates image data at a predetermined frame rate, for example, 15 fps. In the first embodiment, the photographing camera 8 functions as an imaging unit.
  • the control unit 20 controls the drive of the microscope system 100.
  • the control unit 20 controls the illumination of the illumination light of the coaxial epi-illumination light source 5 and the polygonal illumination 6 in accordance with the command received through the operation unit 50.
  • the control unit 20 includes a camera control unit that controls imaging conditions such as an exposure time of the imaging camera 8 and a frame rate, and a polygonal illumination control unit 22 that controls a lighting mode of the polygonal illumination 6 and light amount and the like.
  • the image processing apparatus 30 is configured using a personal computer.
  • the image processing apparatus 30 extracts the luminance information of the calibration image captured by the imaging camera 8 in a state where the lighting part of the segment is changed, and the luminance average which calculates the luminance average value from the luminance information
  • the brightness average value comparison unit that obtains the brightness ratio information for each lighting portion of the segment by comparing the value average of the brightness of the reference image in the calibration image with that of the value calculation unit 32 and the brightness average of the other calibration images.
  • 33 and an image processing control unit 34 that controls each unit of the image processing apparatus 30 and that combines plural image data generated by the photographing camera 8.
  • the image processing apparatus 30 acquires image data generated by the imaging camera 8 and displays the image data on the display unit 60, and receives input of an instruction signal instructing various operations related to the microscope system 100 via the input unit 70.
  • the storage unit 40 is configured using an SDRAM (Synchronous Dynamic Random Access Memory), a flash memory, or the like, and various programs executed by the image processing apparatus 30, data being processed, image data generated by the imaging camera 8, etc. Record.
  • the storage unit 40 includes an image storage unit 41 for storing a calibration image, a luminance information storage unit 42 for storing a luminance average value of the calibration image, and a calibration information storage unit 43 for storing luminance ratio information of the calibration image. And.
  • the storage unit 40 may be configured using a removable memory card or the like from the outside.
  • the display unit 60 is configured using a display panel made of liquid crystal, organic EL (Electro Luminescence), or the like.
  • the display unit 60 displays an image corresponding to the image data generated by the photographing camera 8.
  • the input unit 70 is configured using an input device such as a keyboard and a mouse, and outputs an operation signal to the image processing apparatus 30 according to the operation input of various input devices.
  • the image processing storage unit 23 is configured using an SDRAM (Synchronous Dynamic Random Access Memory), a flash memory, or the like, and various programs executed by the image processing apparatus 30, data being processed, and image data generated by the imaging camera 8 Record etc.
  • the image processing storage unit 23 may be configured using a memory card or the like that is detachable from the outside.
  • FIG. 3 is a flowchart for describing a process of photographing a sample in the microscope system 100 according to the first embodiment of the present invention.
  • FIG. 4 is a flow chart for explaining the calibration image acquiring step of FIG.
  • FIG. 5 is a flow chart for explaining the luminance reference setting process of FIG.
  • FIG. 6 is a flowchart illustrating the brightness adjustment process of FIG.
  • FIG. 7 illustrates the imaging process of FIG.
  • imaging is performed from a calibration image acquisition step (step S1), a luminance reference setting step (step S2), a luminance adjustment step (step S3), and an imaging step (step S4).
  • step S1 a calibration image acquisition step
  • step S2 a luminance reference setting step
  • step S3 a luminance adjustment step
  • step S4 an imaging step
  • step S101 After powering on the microscope system 100, a white sample for calibration is placed on the stage 3 (step S101), and an instruction to start calibration is input. 70 is received (step S102).
  • the exposure time of the imaging camera is set to a predetermined time (step S103), and the lighting pattern of the polygonal illumination 6 and the indication of the lighting site are received from the operation unit 50 (step S104).
  • the lighting part of the polygonal lighting 6 may be at least one LED lighting 61, and may be four LED lighting (FIG. 2A (1)) according to the condition to be observed.
  • the image of the sample is captured by the imaging camera (step S105), and the captured image is stored in the image storage unit 41 as a calibration image (step S106).
  • the luminance information extraction unit 31 extracts luminance information of the calibration image (step S107), and the luminance information storage unit 42 stores the extracted luminance information (step S108).
  • the image processing device 30 determines whether or not calibration images have been acquired for all the lighting sites of the multi-angle illumination 6 (step S109), and when not capturing images for all the lighting sites (step S109: No), The segment to be lit is rotated by the operation unit 50 (step S110), and the process is repeated from step S105. When imaging is performed on all the lighting parts (step S109: Yes), the calibration image processing process is ended.
  • the designation of a reference image for setting the luminance reference of calibration is received from among the images taken in the calibration image acquisition step (step S201).
  • step S202 In order to extract the luminance information of the reference image, the selection of whether the setting of the ROI (Region of Interest) is automatic or manual is accepted (step S202).
  • the ROI specification is manual (step S202: No)
  • the setting of the ROI is accepted through the input unit 70 (step S203).
  • the luminance average value calculation unit 32 calculates the luminance average value of the ROI (step S204). If the ROI is automatic, specify the ROI in the central part of the calibration image.
  • the calibration information storage unit 43 stores the luminance average value of the reference image calculated in step S204 as a calibration reference value (step S205).
  • the designation of the calibration image for calculating the average brightness value is received through the input unit 70 (step S206), and the average brightness value calculation unit 32 calculates the average brightness value of the same ROI as the reference image (step S207).
  • the luminance average value comparison unit 33 compares the luminance average values of the reference image and the calibration image (step S208), and the calibration information storage unit 43 stores the comparison result as luminance ratio information (step S209).
  • the image processing apparatus 30 determines whether or not the luminance ratio information of all calibration images is acquired (step S210), and when the luminance ratios of all calibration images are not acquired (step S210: No) , And repeat from step S206.
  • the luminance ratio of all calibration images is acquired (step S210: Yes)
  • the luminance reference setting process is ended.
  • the light amount of the polygonal illumination 6 is adjusted based on the brightness ratio information (step S301).
  • the polygonal illumination control unit 22 acquires luminance ratio information from the calibration information storage unit 43, and adjusts the light amount of the LED illumination 61.
  • step S302 the lighting instruction of the segment of the polygonal lighting 6 is received from the operation unit 50 (step S302).
  • the image of the sample is photographed by the photographing camera (step S303), and the photographed image is stored as a calibration image in the image storage unit 41 (step S304), and the luminance information extraction unit 31 obtains the luminance information of the calibration image.
  • the luminance information storage unit 42 stores the extracted luminance information (step S306).
  • the brightness average value calculation unit 32 calculates the brightness average value of the ROI from the brightness information extracted in step S305 (step S307), and whether the obtained brightness average value is within a predetermined range from the calibration reference value. Is determined (step S308). When the difference between the obtained luminance average value and the calibration reference value is out of the predetermined range (step S308: No), it is determined whether the polygonal illumination control unit 22 is the limit value that can set the light amount of the current LED light source 61. Is determined (step S309), and if it is the limit value (step S309: Yes), the display unit 60 is notified of that (step S310).
  • step S309 the brightness average value comparison unit 33 corrects the brightness ratio information so as to compensate for the excess or deficiency of the brightness (step S311), and repeats from step S301.
  • the image processing apparatus 30 determines whether the luminance comparison with the calibration reference value has been performed in all the lighting parts of the polygonal illumination 6 (step S312), and the luminance comparison is not performed in all the lighting parts ((S312) Step S312: No), the segment to be lit is rotated by the operation unit 50 (step S313), and the process is repeated from step S303.
  • the brightness comparison is performed for all the lighting parts (step S312: Yes)
  • the brightness adjustment process is ended.
  • step S 401 after replacing the white background sample for calibration on the stage 3 with the sample for imaging (step S 401), lighting of the polygonal illumination 6 input through the operation unit 50 is performed The instruction of the pattern and the lighting site is received (step S402).
  • the polygonal illumination control unit 22 acquires luminance ratio information corresponding to the set lighting pattern from the calibration information storage unit 43 (step S403), and adjusts the light amount of the LED illumination 61 based on the acquired luminance ratio information ((step S403) Step S404).
  • the image of the sample 2 is photographed by the photographing camera 8 (step S405), and the image storage unit 41 stores the photographed image (step S406).
  • the image processing apparatus 30 determines whether or not the image has been acquired at all the lighting sites of the polygonal illumination 6 (step S407), and when not capturing at all the lighting sites (step S407: No), the operation unit The segment to be lit is rotated by 50 (step S408), and the process is repeated from step S405. When imaging has been performed on all the lighting parts (step S407: Yes), the imaging process is ended.
  • the light amount of the illumination applied to the sample can be made constant. Thereby, the luminance of the image obtained by observation becomes uniform, and reflection after image processing and reflection of the light source can be reduced.
  • the light amount of the LED illumination 61 to be irradiated to the sample is adjusted by calibration, it is sufficient if the imaging conditions can be made uniform for each lighting part of the polygonal illumination 6. It is also possible to adjust the exposure time of each of the lighting portions of the polygon illumination 6 to make the luminance of the image obtained by observation uniform. Alternatively, both the light amount of the polygonal illumination 6 and the exposure time of the photographing camera 8 may be adjusted.
  • the brightness average value comparing unit 33 uses the brightness average value calculated by the brightness average value calculating unit 32 to calculate the brightness average value of the reference image in the calibration image and the other calibrations.
  • the average luminance values of the calibration images are compared, and the luminance ratio information is acquired for each lighting part of the segment.
  • luminance ratio information can be acquired for each of the lighting parts of. Therefore, instead of the luminance average value, luminance ratio information between the reference image and the calibration image can also be acquired from the luminance median value or the luminance adjustment average value.
  • the polygonal illumination 8 having 16 LED illuminations 61 is used, the present invention is not limited to this, and the number of LED illuminations 61 is two, four, eight, thirty two , 64 etc. may be sufficient.
  • the lighting mode of the LED lighting 61 is not limited to the one described above, and may be a mode in which the LED lighting 61 is turned on by 1/8, a mode in which it is turned on by 3/16, or the like.
  • the arrangement of the LED illumination 61 is not limited to a circle, and a geometric shape such as a polygon may be employed.
  • FIG. 8 is a view showing an example of the image combining screen 61. As shown in FIG.
  • the image combining screen 61 has an image group display unit 62 that displays thumbnails 62 a of a plurality of images captured for each lighting site, and a brightness reference bar 63 that selects the brightness of the image to be combined.
  • the calibration process by performing the calibration process, it is possible to significantly reduce the variation in the amount of light irradiated to the sample 2 due to the hardware. Inclusions may remain. In such a case, reflection and reflection can be reduced by adjusting the brightness of the image by image processing.
  • reflection may remain.
  • both the reflection and the reflection can be reduced by setting the target of the automatic exposure of the imaging camera 8 high, acquiring the image, and combining the image based on the low luminance standard.
  • the microscope system 100A according to the second embodiment includes a sensor 11a that detects a hole position of the revolver 11, and a revolver information detection unit 23.
  • FIG. 9 is a schematic view showing a schematic configuration of a microscope system 100A according to the second embodiment.
  • the revolver 11 to which the plurality of objective lenses 4 are attached has a hole position of the revolver 11, that is, a sensor 11a that detects the objective lens on the optical path.
  • the control unit 20A detects the hole position detected by the sensor 11a. It has a revolver information detection unit 23 that stores information.
  • the sensor 11 a and the revolver information detection unit 23 constitute a detection unit.
  • FIG. 10 is a flow chart for explaining the imaging process of the sample 2 in the microscope system 100A according to the second embodiment of the present invention.
  • the hole position information of the revolver is detected by the sensor 11a and the revolver information detection unit 23 (step S501).
  • the image processing apparatus 30 determines whether the hole position of the revolver on the optical path has been calibrated (step S502). If calibration has been performed (step S502: YES), an imaging process is performed (step S503).
  • the imaging process of step S503 is the same as the imaging process of the first embodiment.
  • step S504 If calibration has not been performed (step S502: No), a calibration image acquisition process (step S504), a luminance reference setting process (step S505), and a luminance adjustment process (step S506) are performed.
  • the processes in steps S504 to S506 are the same as those in the first embodiment, and the image storage unit 41A, the luminance information storage unit 42A, and the calibration information storage unit 43A are configured to store an image, luminance information, and a calibration reference value.
  • the luminance ratio information including is stored for each hole position of the revolver 11 respectively.
  • FIG. 11 is a diagram for explaining an image display screen 80 displayed on the display unit 60 in the microscope system 100A according to the second embodiment of the present invention.
  • the image display screen 80 includes a preview display unit 81 for displaying the selected image preview, an image group display unit 82 for displaying thumbnails 82a of a plurality of images, and a hole position selection for selecting the hole position of the revolver 11 of the image to be displayed. And a lighting portion display portion 84 for displaying a lighting portion of the polygonal illumination 6.
  • FIG. 12 is a flowchart of image preview display using the image display screen 80 of FIG.
  • the selection portion 83a of the hole position selection portion 83 is designated using the input portion 70 such as a mouse etc. by the cursor etc. displayed in the hole position selection portion 83 (step S601), and at the designated hole position of the revolver 11
  • the thumbnail 82a of the photographed image is displayed on the image group display unit 82 (step S602).
  • step S603 When the thumbnail 82a displayed on the image group display unit 82 is selected through the input unit 70 (step S603), the lighting portion 84a of the polygonal illumination 6 of the selected thumbnail 82a is displayed on the lighting portion display unit 84 (step S604) A preview of the selected image (thumbnail 82a) is displayed on the preview display unit 81 (step S605).
  • FIG. 13 is a flowchart of another form of image preview display using the image display screen 80 of FIG.
  • the selection portion 83a of the hole position selection portion 83 is designated using the input portion 70 such as a mouse displayed on the hole position selection portion 83 (step S701), and at the designated hole position of the revolver 11
  • the thumbnail 82a of the photographed image is displayed on the image group display unit 82 (step S702).
  • the lighting part 84 a of the polygonal illumination 6 is designated using the input unit 70 such as a cursor displayed on the lighting part display unit 84 (step S 703).
  • the thumbnails 82a displayed in the image group display unit 82 are marked (step S704), and the preview of the marked image (thumbnail 82a) is the preview display unit 81 Is displayed (step S 705).

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Abstract

La présente invention concerne un système de microscope permettant de réduire la réflexion ou l'éblouissement par uniformisation de la quantité de lumière de chaque éclairage émis vers un échantillon dans un éclairage à angles multiples comportant une pluralité d'éclairages. Un système de microscope 100 est caractérisé en ce qu'il est pourvu de : un éclairage à angles multiples 6 comportant deux segments ou plus pour émettre de la lumière, l'éclairage à angles multiples 6 modifiant une partie éclairée des segments et émettant de la lumière ayant différents motifs d'irradiation vers un échantillon 2; une unité opérationnelle 50 pour recevoir une définition du motif d'irradiation ou similaire de l'éclairage à angles multiples 6; un objectif 4 pour collecter la lumière d'une image d'observation de l'échantillon 2; une caméra d'imagerie 8 pour capturer une image de l'échantillon 2; une unité d'extraction d'informations de luminance 31 pour extraire des informations de luminance d'une image d'étalonnage; une unité de calcul de valeur moyenne de luminance 32 pour calculer une valeur moyenne de luminance à partir des informations de luminance, une unité de comparaison de valeur moyenne de luminance 33 pour comparer la valeur moyenne de luminance d'une image de référence et d'une autre image d'étalonnage et acquérir des informations de rapport de luminance pour chaque partie éclairée des segments; et une unité de commande 20 pour commander une condition d'imagerie de l'échantillon 2 sur la base des informations de rapport de luminance.
PCT/JP2018/022475 2017-06-15 2018-06-12 Système de microscope WO2018230575A1 (fr)

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Citations (6)

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