WO2018196055A1 - 高分子材料表面改性方法及其产品和用途 - Google Patents
高分子材料表面改性方法及其产品和用途 Download PDFInfo
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- WO2018196055A1 WO2018196055A1 PCT/CN2017/084811 CN2017084811W WO2018196055A1 WO 2018196055 A1 WO2018196055 A1 WO 2018196055A1 CN 2017084811 W CN2017084811 W CN 2017084811W WO 2018196055 A1 WO2018196055 A1 WO 2018196055A1
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- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08J—WORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G or C08H
- C08J7/00—Chemical treatment or coating of shaped articles made of macromolecular substances
- C08J7/12—Chemical modification
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- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08J—WORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G or C08H
- C08J2327/00—Characterised by the use of homopolymers or copolymers of compounds having one or more unsaturated aliphatic radicals, each having only one carbon-to-carbon double bond, and at least one being terminated by a halogen; Derivatives of such polymers
- C08J2327/02—Characterised by the use of homopolymers or copolymers of compounds having one or more unsaturated aliphatic radicals, each having only one carbon-to-carbon double bond, and at least one being terminated by a halogen; Derivatives of such polymers not modified by chemical after-treatment
- C08J2327/12—Characterised by the use of homopolymers or copolymers of compounds having one or more unsaturated aliphatic radicals, each having only one carbon-to-carbon double bond, and at least one being terminated by a halogen; Derivatives of such polymers not modified by chemical after-treatment containing fluorine atoms
- C08J2327/18—Homopolymers or copolymers of tetrafluoroethylene
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- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08J—WORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G or C08H
- C08J2361/00—Characterised by the use of condensation polymers of aldehydes or ketones; Derivatives of such polymers
- C08J2361/04—Condensation polymers of aldehydes or ketones with phenols only
- C08J2361/16—Condensation polymers of aldehydes or ketones with phenols only of ketones with phenols
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- the invention relates to a surface modification method of a polymer material, and a product and a use thereof, the surface modification method particularly relates to a surface of a medical polymer material treated by plasma immersion ion implantation and a covalently grafted organism on the surface after treatment Active molecules to achieve their biochemical modification.
- Polymer materials also known as polymer materials, are widely used in the biomedical field for the repair, replacement and regeneration of living tissues, as well as for care and medical appliances. Due to its special application environment (need to directly contact with human body fluids, blood, organs, tissues, etc.), biomedical polymer materials must have good physical and mechanical properties, chemical stability, non-toxicity, and easy processing and formability. It must also have biocompatibility, medical functionality, and the like. Since the biological properties of medical polymer materials are mainly determined by the physicochemical properties of their surfaces, surface modification can improve their biological properties or give them some special biology without affecting their own advantages. Features.
- biomimetic modification of medical polymer materials that is, biologically active macromolecules such as proteins, polysaccharides, polypeptides, cell growth factors, etc.
- the surface forms a stable bio-transition layer that interacts specifically with the biological environment to further enhance the biological properties of the material or confer specific biological functions.
- the grafting of heparin and hirudin on the surface of artificial blood vessels constructed of polytetrafluoroethylene can achieve the effect of inhibiting thrombus formation, thereby greatly improving the long-term patency rate of artificial blood vessels (Hoshi R A, Van Lith R, Jen M C, Et al.
- wet chemical crosslinking techniques is not only relatively complicated and cumbersome, but also introduces toxic organic crosslinking reagents. More importantly, this chemical method is difficult to graft a variety of bioactive molecules on the surface at the same time, and the sequential grafting of multiple biomolecules makes the grafting process extremely cumbersome and difficult to achieve.
- the object of the present invention is to provide a simple and feasible method for surface modification biochemical modification of a polymer material, and a product and use thereof, the surface modification method particularly relates to a medical treatment based on gas plasma immersion ion implantation (ie, gas PIII)
- gas PIII gas plasma immersion ion implantation
- the bioactive molecule is covalently grafted onto the surface of the molecular material and on the treated surface to effect its biochemical modification.
- the modification method provided by the invention does not need to use a chemical crosslinking agent, firstly performs surface modification and activation of the medical biopolymer material by gas plasma immersion ion implantation, and then immerses the modified medical polymer material in containing biomolecules. After incubation for a period of time, the biologically active macromolecules can be covalently grafted onto the surface of the polymer material to achieve biochemical surface modification.
- the grafting principle of the biomolecule on the surface of the medical material in the present invention is: in the process of modifying the polymer material by using the gas plasma immersion ion implantation technique, the collision of the injected high energy ions with the polymer chain and the charge and energy The transfer produces a large number of long-acting and highly active free radicals (Free Radical) on the surface of the polymer material.
- Free Radical free radicals formed by high-energy ion bombardment can be preserved for a long time in the affected area of ion implantation and continuously migrate to the surface of the substrate.
- the free radicals that reach the surface react with the molecules present in the environment and ultimately covalently graft the molecules onto the surface of the material.
- the surface modification technical scheme adopted by the present invention can be divided into two steps: 1. gas plasma immersion ion implantation treatment; 2. incubation in a solution containing biomolecules.
- a negative bias is applied to the sample disk used to accelerate the positively charged ions in the plasma and eventually inject them into the surface of the material.
- the process parameters used for gas plasma immersion ion implantation include: background vacuum of 5 ⁇ 10 -4 to 9 ⁇ 10 -3 Pa, gas introduction flow rate of 10 to 200 sccm, and negative bias voltage of the sample tray of 5 to 50 kV.
- the injection pulse width is 10 to 300 microseconds
- the injection pulse frequency is 50 to 2000 Hz
- the radio frequency power is 100 to 3000 W
- the injection time is 30 to 300 minutes.
- the preferred parameters are: the background vacuum is 1 ⁇ 10 -3 to 9 ⁇ 10 -3 Pa, the gas introduction flow rate is 20-100 sccm, the negative bias voltage of the sample tray is 10-30 kV, and the injection pulse width is 20-200.
- the microsecond has an injection pulse frequency of 50 to 500 Hz, a radio frequency power of 100 to 1000 W, and an injection time of 30 to 180 minutes.
- the most preferred parameters are: background vacuum of 5 ⁇ 10 -3 Pa, gas introduction flow rate of 30 sccm, sample tray with a negative bias of 15 kV, injection pulse width of 20 ⁇ sec, injection pulse frequency of 500 Hz, RF power It is 1000W and the injection time is 60-180 minutes.
- the gas used in the gas plasma immersion ion implantation is not particularly required, and it is preferred to use a non-toxic gas for the needs of the field of application of the present invention.
- Different gases may affect the density of surface free radicals after material modification, which affects the efficiency of grafting.
- common gases such as oxygen, nitrogen, argon, ammonia, and hydrogen are used. Feasible of the invention Sex.
- this method can be applied to all polymer materials that can withstand gas PIII technology, such as polyethylene, polytetrafluoroethylene, polystyrene, polyvinyl chloride, polypropylene, polyamide, polyetheretherketone (abbreviation). PEEK), polylactic acid, polyglycolic acid, polyurethane, poly(lactic-co-glycolic acid) copolymer, polybutylene succinate, polycaprolactone, polymethyl methacrylate, epoxy resin, polychlorinated Ethylene and the like.
- gas PIII technology such as polyethylene, polytetrafluoroethylene, polystyrene, polyvinyl chloride, polypropylene, polyamide, polyetheretherketone (abbreviation). PEEK), polylactic acid, polyglycolic acid, polyurethane, poly(lactic-co-glycolic acid) copolymer, polybutylene succinate, polycaprolactone, polymethyl methacrylate, epoxy resin, polych
- polyetheretherketone As an FDA-approved medical implant material, polyetheretherketone is not only light, bio-stable and non-biotoxic, but more importantly its elastic modulus (5-8GPa) than metal orthopedic implant materials. Closer to the human bones, and after being implanted in the body, X-ray transmission, MRI and computed tomography do not produce artifacts and many other biomedical advantages. However, PEEK is an inert biological material, and the lack of biocompatibility causes its Osseointegration to be insufficient after implantation and requires secondary surgery for correction.
- polytetrafluoroethylene has excellent chemical stability, and thus it is difficult to carry out surface modification by chemical methods alone.
- the polymer material in the present invention is preferably polytetrafluoroethylene or polyetheretherketone.
- the polymer material treated by the gas plasma immersion ion implantation is incubated in a solution containing biomolecules.
- the solution containing biomolecules is a buffer system capable of keeping biomolecules active, such as phosphate buffered saline (PBS) or physiological saline.
- PBS phosphate buffered saline
- physiological saline a buffer system capable of keeping biomolecules active, such as phosphate buffered saline (PBS) or physiological saline.
- the temperature and time of incubation in a solution containing biomolecules is such that biomolecules remain biologically active during incubation.
- the temperature of the incubation is usually selected at 4-37 ° C; the incubation time ensures that the grafting is completed; preferred conditions are, for example, incubation in a solution containing biomolecules at 4 ° C for more than 12 hours.
- the loading of the biomolecule on the surface of the polymer material can be adjusted by the concentration of the biological solution used at the time of incubation, and the concentration of the biological solution is, for example, 10 to 2000 ⁇ g/mL, preferably 50 to 500 ⁇ g/mL.
- Simultaneous loading of two or more biomolecules can be achieved by incubating for a period of time in a co-mixed solution of the corresponding biomolecule.
- biomolecules can be carried out according to this, or the grafting can be carried out one or more times by separating the different parts and incubating with the corresponding different biomolecule solutions.
- Grafting of different biomolecules can achieve different functions, such as polysaccharides (such as heparin), peptides (such as hirudin), proteins (such as horseradish peroxidase, transmembrane glycoprotein CD47), and cytokines. (such as human stromal cell-derived factor 1 ⁇ (SDF-1 ⁇ )) and the like.
- polysaccharides such as heparin
- peptides such as hirudin
- proteins such as horseradish peroxidase, transmembrane glycoprotein CD47
- cytokines such as human stromal cell-derived factor 1 ⁇ (SDF-1 ⁇ )
- the surface of the polymer material immersed in the gas plasma immersion ion implantation is long-term effective in the ability to covalently load biomolecules by incubation in a solution containing biomolecules while being stored in the air.
- the surface obtained by the method of the present invention is a biomolecule modified polymer material having a structure in which a surface layer of a polymer material has active radicals introduced by gas plasma immersion ion implantation, which react with biomolecules and Covalently grafted onto the surface of the material.
- the present invention uses a gas plasma immersion ion implantation technique to treat a biomedical polymer material, and can covalently graft a bioactive macromolecule onto a surface of the material without using a chemical crosslinking agent. To achieve its biochemical surface modification.
- the present invention has the following advantages:
- Plasma immersion ion implantation technology eliminates the "line of sight limitation" of conventional beam line ion implantation.
- the injection process is omnidirectional, and even surface samples with uniform morphology can be uniformly surface treated.
- Plasma immersion ion implantation treatment can greatly improve the surface physicochemical properties, such as surface roughness, surface energy and surface chemical composition, without affecting the performance of polymer matrix. And using different gases for injection treatment, different surface physicochemical properties can be obtained.
- the surface of the polymer material does not need to be treated with a chemical crosslinking agent, and only needs to be immersed in a solution containing biomolecules for a period of time to covalently load the bioactive molecules in the solution.
- the surface modification method not only has simple and convenient operation, but also avoids the use of toxic chemical cross-linking agent, and is favorable for large-scale and industrial production.
- the loading of biomolecules on the surface of the polymer material can be adjusted by the concentration of the solution used for incubation. And when a co-mixed solution of a plurality of biomolecules is used, a corresponding plurality of biomolecules can be covalently grafted to the surface of the polymer material after the plasma immersion ion implantation treatment.
- Gas plasma immersion ion implantation Surface modified polymer material has no special requirements on the storage conditions after treatment. Even if stored in the air for a long time, its ability to covalently graft biomolecules by incubation in a solution containing biomolecules does not decrease significantly.
- the biomimetic modification method for biomedical polymer materials provided by the invention has wide application prospects in the fields of medical implant materials, functional materials, bioactive materials, etc., and has simple process, low cost, and is suitable for batch and industrial production. .
- Figure 1a is a scanning electron micrograph of the surface of a polytetrafluoroethylene that has not been subjected to plasma immersion ion implantation in Example 1.
- Figure 1b is a scanning electron micrograph of the surface of a polytetrafluoroethylene treated by nitrogen plasma immersion ion implantation in Example 1.
- Figure 1c is a scanning electron micrograph of the surface of a polytetrafluoroethylene treated by ammonia gas plasma immersion ion implantation in Example 1.
- Fig. 1d is a scanning electron micrograph of the surface of polytetrafluoroethylene treated by nitrogen gas plasma immersion ion implantation in Example 1.
- Example 2a is a three-dimensional picture, a cross-sectional profile, and its corresponding root mean square roughness (RMS) of an atomic force microscope of the surface of a polytetrafluoroethylene that has not been subjected to plasma immersion ion implantation in Example 2.
- RMS root mean square roughness
- 2b is a three-dimensional picture, a cross-sectional profile, and its corresponding root mean square roughness (RMS) of the surface of a polytetrafluoroethylene treated by nitrogen plasma immersion ion implantation in Example 2.
- RMS root mean square roughness
- 2c is a three-dimensional picture, cross-sectional profile, and its corresponding root mean square roughness (RMS) of the surface of the polytetrafluoroethylene treated by ammonia gas plasma immersion ion implantation in Example 2.
- RMS root mean square roughness
- 2d is a three-dimensional picture, a cross-sectional profile, and its corresponding root mean square roughness (RMS) of an atomic force microscope of the surface of a polytetrafluoroethylene treated by nitrogen gas plasma immersion ion implantation in Example 2.
- RMS root mean square roughness
- FIG. 3 is a full spectrum of X-ray photoelectron spectroscopy of the surface of a polytetrafluoroethylene material before and after gas plasma immersion ion implantation in Example 3.
- FIG. 3 is a full spectrum of X-ray photoelectron spectroscopy of the surface of a polytetrafluoroethylene material before and after gas plasma immersion ion implantation in Example 3.
- Example 4 is a graph showing the static contact angle of the surface of the polytetrafluoroethylene material before and after the gas plasma immersion ion implantation treatment in Example 4.
- Fig. 5 is a graph showing the heparin loading density of the surface of the sample before and after the gas plasma immersion ion implantation treatment in Example 5 after incubation in a heparin solution.
- Example 6 is a surface heparin loading density after the sample which has been subjected to gas plasma immersion ion implantation in Example 6 after being stored in the air for a corresponding period of time and then incubated in the heparin solution.
- Figure 7 is a graph showing the long-term stability of heparin in the surface of polytetrafluoroethylene treated with nitrogen plasma immersion ion implantation in Example 7.
- Figure 8 is a surface of Example 8 after plasma ion immersion ion implantation treatment and untreated surface after elution with phosphate buffer or 2% sodium dodecyl sulfate after loading horseradish peroxidase Retention of horseradish peroxidase.
- Example 9 is a surface of the sample treated by each gas plasma immersion ion implantation in Example 9 after being stored in the air for a corresponding period of time and then loaded with horseradish peroxidase and eluted by 2% sodium dodecyl sulfate. Retention of horseradish peroxidase.
- Figure 10 is a table of oxygen plasma immersion ion implantation treatment and non-treatment (PEEK control) in Example 10.
- a sheet of polytetrafluoroethylene having a diameter of 15 mm and a thickness of 0.1 mm was sequentially ultrasonically cleaned with acetone, alcohol, and deionized water. This pretreated sample is called PTFE control.
- the pretreated polytetrafluoroethylene is treated by a gas plasma immersion ion implantation technique.
- the treatment is carried out by a method of injecting ammonia gas by nitrogen injection, ammonia injection, or nitrogen injection.
- the specific treatment process is as follows: the background vacuum is 5 ⁇ 10 -3 Pa, the gas introduction flow rate is 30 sccm, the sample disk is loaded with a negative bias voltage of 15 kV, the injection pulse width is 20 microseconds, the injection pulse frequency is 500 Hz, and the RF power is It is 1000W.
- the injection time of nitrogen gas is 180 minutes, the sample after the treatment is called N2PIII; the injection time of ammonia gas is 60 minutes, the sample after the treatment is called NH3PIII; after the injection of nitrogen for 180 minutes, the injection of ammonia gas is injected for 60 minutes.
- This treated sample is referred to as N2+NH3PIII. (the same below)
- the surface of the polytetrafluoroethylene before and after the gas plasma immersion ion implantation treatment was observed by a scanning electron microscope to obtain a photograph of the surface topography shown in FIG. It can be seen from Fig. 1 that the gas plasma immersion ion implantation treatment changes the surface morphology of the polytetrafluoroethylene to varying degrees.
- the untreated PTFE control surface shown in Figure 1a is substantially flat, while the surface of the N2PIII sample after the nitrogen plasma injection shown in Figure 1b exhibits a quasi-regular nanoscale "valley"-like structure.
- FIG. 1c is similar to the PTFE control surface, and the surface of the N2+NH3PIII sample after the nitrogen injection shown in Fig. 1d is similar to the surface of the N2PIII.
- the ammonia gas plasma injection did not change the surface morphology of the polytetrafluoroethylene.
- the surface roughness of the polytetrafluoroethylene sample obtained in the treatment of Example 1 was characterized by atomic force microscopy to obtain the three-dimensional picture, profile profile and corresponding mean square roughness (RMS) results of the surface shown in FIG. It can be seen from the three-dimensional picture of the surface in Fig. 2 that the results obtained by the atomic force microscope are substantially the same as those obtained by the scanning electron microscope in Example 1.
- the surface roughness of the PTFE control shown in Figure 2a is 17.9 nm. Nitrogen plasma implantation significantly increased the surface roughness of the sample to 134 nm (Fig. 2b). The ammonia gas plasma injection had no effect on the surface roughness, and the mean square roughness after the treatment was 18.2 nm (Fig. 2c).
- the surface mean square roughness of the ammonia gas injection after the nitrogen plasma injection was substantially the same as that of the nitrogen gas alone, and was 130 nm (Fig. 2d).
- X-ray photoelectron spectroscopy wide field scanning was performed on the surface of the polytetrafluoroethylene sample obtained in Example 1, and the XPS full spectrum spectrum shown in Fig. 3 was obtained.
- the abscissa indicates the binding energy and the ordinate indicates the peak intensity.
- the nitrogen plasma immersion ion implantation site is known.
- the fluorine content on the surface of the PTFE is reduced, and oxygen is introduced on the surface of the material; and the ammonia plasma immersion ion implantation treatment significantly reduces the surface fluorine content and significantly increases the surface nitrogen element. And the proportion of oxygen.
- the surface wettability of the material was tested using a static water contact angle tester (Rame'-Hart instrument), and 5 ⁇ L of ultrapure water was slowly dropped vertically onto the sample surface by a syringe, and the machine's own imaging system was used to take a photograph of the droplet and analyze the contact. Angle size. Three pieces of material were used for each group, and five measurement data were averaged on each sample.
- FIG. 4 is a graph showing the static contact angle of the surface of the polytetrafluoroethylene before and after the modification treatment in Example 1, wherein the abscissa is the sample name and the ordinate is the degree of the contact angle. It can be seen from Fig. 4 that the contact angle of the untreated PTFE control is 115°, and the contact angle is increased to 147° after the nitrogen plasma treatment. The ammonia gas plasma treatment reduces the contact angle to 42°, and the nitrogen plasma The surface contact angle after treatment with ammonia gas was approximately super-hydrophilic at 13°.
- the modified polytetrafluoroethylene sample of Example 1 was immersed in a phosphate buffer solution containing heparin, wherein the concentration of heparin was 500 ⁇ g/mL, and stored at 4 ° C for 12 hours. The sample was then removed from the heparin solution and the sample was rinsed with heparin-free phosphate buffer to remove heparin that was not grafted.
- the heparin loading on the surface of each sample was measured by toluidine blue colorimetric method, and the results shown in Fig. 5 were obtained, in which the abscissa is the sample name and the ordinate is the heparin loading density.
- the untreated PTFE control cannot load heparin, and the sample ion-implanted by plasma immersion can effectively load heparin on the surface despite a certain difference in the load.
- Example 6 After the modified polytetrafluoroethylene sample in Example 1 was stored in the air for 72 days or 198 days, the heparin loading was again incubated in the heparin solution according to the method of Example 5, and a graph was obtained.
- the samples after the plasma treatment were stored in the air for half a year, their ability to load heparin in the heparin solution did not disappear, and the overall improvement was somewhat improved.
- Example 5 The sample after heparin-loaded with nitrogen plasma in Example 5 was further immersed in a phosphate buffer solution containing no heparin, and stored at 37 ° C for a certain period of time, and then taken out, and the heparin loading amount on the surface was measured, and the result shown in FIG. As a result, the abscissa is the soaking time of the sample and the ordinate is the loading density of heparin.
- the surface was treated with nitrogen plasma to have long-term stability. The surface heparin loading remained unchanged after soaking in phosphate buffer for up to 28 days. This aspect indicates that the loaded heparin has not been released, and on the other hand, heparin has not been denatured and inactivated during this process.
- the modified polytetrafluoroethylene sample in Example 1 was immersed in a phosphate buffer solution containing horseradish peroxidase, wherein the horseradish peroxidase concentration was 50 ⁇ g/mL, and at 4 ° C Store under conditions for 12 hours. The sample was then removed from the solution and the sample was eluted with horseradish peroxidase-free phosphate buffered saline (PBS) for 1 hour or with 2% sodium lauryl sulfate (2% SDS). The sample was taken off for 1 hour.
- PBS horseradish peroxidase-free phosphate buffered saline
- SDS sodium lauryl sulfate
- the eluted sample was then placed in a 24-well cell culture plate, and 500 ⁇ l of 3,3',5,5'-tetramethylbenzidine solution was added to each well and incubated for 3 minutes at room temperature, followed by 500 micron. A 2 mol/L hydrochloric acid solution was added to terminate the reaction. 200 ⁇ l of each well was taken out into a 96-well culture plate and the amount of horseradish peroxidase loaded on the surface of each sample was measured by measuring the absorbance at a wavelength of 450 nm on a microplate reader. The experimental results are shown in Fig. 8. In the figure, the abscissa is the elution method after incubation of each sample in horseradish peroxidase solution, and the ordinate is the absorbance at 450 nm.
- the PTFE control sample which has not been subjected to plasma treatment can be loaded with a certain amount of horseradish peroxidase on its surface after being incubated for a while in the horseradish peroxidase solution.
- the loaded enzyme can withstand the elution of phosphate buffer, the elution of sodium lauryl sulfate completely removes the enzyme supported on the surface, demonstrating that the enzyme supported on the PTFE control surface is only physically adsorbed on the surface of the material.
- the enzyme loaded on the surface of the sample after each plasma treatment can be subjected to the elution of phosphate buffer and sodium lauryl sulfate, which proves that the enzyme is supported by covalent grafting because sodium lauryl sulfate is a kind.
- the modified polytetrafluoroethylene sample in Example 1 was stored in air for 72 days or 198 days, and again incubated in the horseradish peroxidase solution according to the method of Example 8 and passed through the examples.
- the method described in 8 detects the relative loading of horseradish peroxidase, and the results shown in Fig. 9 are obtained, wherein the abscissa is the storage time of the sample in air and the ordinate is the absorbance at 450 nm.
- the samples after plasma treatment were stored in the air for half a year, their ability to covalently graft horseradish peroxidase by incubation did not decrease significantly.
- a polyetheretherketone sheet having a diameter of 15 mm and a thickness of 2 mm was ultrasonically cleaned successively with acetone, alcohol, and deionized water. This pretreated sample is called PEEK control.
- the pretreated polyetheretherketone is treated with oxygen plasma immersion ion implantation (O2PIII).
- O2PIII oxygen plasma immersion ion implantation
- the specific treatment process is as follows: the background vacuum is 5 ⁇ 10 -3 Pa, the gas introduction flow rate is 30 sccm, the sample disk is loaded with a negative bias voltage of 15 kV, the injection pulse width is 20 microseconds, the injection pulse frequency is 500 Hz, and the RF power is It is 1000W and the processing time is 180 minutes.
- This treated sample is referred to as O2PIII.
- the treated polyetheretherketone sample is immersed in a phosphate buffer solution containing horseradish peroxidase, wherein the horseradish is passed through
- concentration of the oxide enzyme was 50 ⁇ g/mL, and it was stored at 4 ° C for 12 hours.
- the sample was then removed from the solution and the sample was eluted with horseradish peroxidase-free phosphate buffered saline (PBS) for 1 hour or with 2% sodium lauryl sulfate (2% SDS). The sample was taken off for 1 hour.
- PBS horseradish peroxidase-free phosphate buffered saline
- SDS sodium lauryl sulfate
- the eluted sample was then placed in a 24-well cell culture plate, and 500 ⁇ l of 3,3',5,5'-tetramethylbenzidine solution was added to each well and incubated for 3 minutes at room temperature, followed by 500 micron. A 2 mol/L hydrochloric acid solution was added to terminate the reaction. 200 ⁇ l of each well was taken out into a 96-well culture plate and the amount of horseradish peroxidase loaded on the surface of each sample was reflected by measuring the absorbance at a wavelength of 450 nm on a microplate reader. The experimental results are shown in Fig. 10. In the figure, the abscissa is the elution method after incubation of each sample in horseradish peroxidase solution, and the ordinate is the absorbance at 450 nm.
- the surface-loaded enzyme of the plasma-free PEEK control sample can withstand the elution of phosphate buffer, but sodium lauryl sulfate.
- the elution completely removes the enzyme loaded on the surface, proving that the enzyme is only physically adsorbed on the surface of the material.
- the enzyme supported on the surface of the sample after O2PIII treatment was able to withstand the elution of phosphate buffer and sodium lauryl sulfate, which proved that the enzyme was supported by covalent grafting.
Abstract
Description
Claims (16)
- 一种基于等离子体浸没离子注入技术的高分子材料表面改性方法,其特征在于,包括如下步骤:1)通过气体等离子体浸没离子注入处理高分子材料;2)将处理过的高分子材料在含有生物分子的溶液中进行孵育;从而将生物分子以共价的形式接枝到高分子材料的表面,其中,所述气体选自无毒的气体;所述高分子材料选自能够经受等离子体浸没离子注入技术处理的高分子材料。
- 根据权利要求1所述的一种基于等离子体浸没离子注入技术的高分子材料表面改性方法,其特征在于,所述高分子材料选自聚乙烯、聚四氟乙烯、聚苯乙烯、聚氯乙烯、聚丙烯、聚酰胺、聚醚醚酮、聚乳酸、聚乙醇酸、聚氨酯、聚(乳酸-羟基乙酸)共聚物、聚丁二酸丁二醇酯、聚已内酯、聚甲基丙烯酸甲酯、环氧树脂。
- 根据权利要求1所述的一种基于等离子体浸没离子注入技术的高分子材料表面改性方法,其特征在于,所述生物分子选自多糖、多肽、蛋白质、细胞因子。
- 根据权利要求3所述的一种基于等离子体浸没离子注入技术的高分子材料表面改性方法,其特征在于,所述多糖选自肝素,多肽选自水蛭素,蛋白质选自辣根过氧化物酶或跨膜糖蛋白CD47,细胞因子选自人基质细胞衍生因子1α。
- 根据权利要求1或2所述的一种基于等离子体浸没离子注入技术的高分子材料表面改性方法,其特征在于,所述等离子体浸没离子注入处理所使用的气体选自氩气、氮气、氨气、氧气、氢气。
- 根据权利要求1或2所述的一种基于等离子体浸没离子注入技术的高分子材料表面改性方法,其特征在于,所述等离子体浸没离子注入处理中本底真空度为1×10-3~9×10-3Pa,气体引入流量为20~100sccm,样品盘所加负偏压为10~30kV。
- 根据权利要求6所述的一种基于等离子体浸没离子注入技术的高分子材料表面改性方法,其特征在于,所述等离子体浸没中本底真空度为5×10-3Pa,气体引入流量为30sccm,样品盘所加负偏压为15kV,注入脉宽为20微秒,注入脉冲频率为500Hz,产生等离子体所使用的射频功率为1000W,注入时间为60~180分钟。
- 根据权利要求1或3所述的一种基于等离子体浸没离子注入技术的高分子材料表面改性方法,其特征在于,所述含有生物分子的溶液为溶解了生物分子并能使其保持活性的缓冲液体系,在含有生物分子的溶液中孵育的温度和时间需保证孵育期间生物分子能够保持生物活性。
- 根据权利要求8所述的一种基于等离子体浸没离子注入技术的高分子材料表面改性方法,其特征在于,所述使生物分子保持活性的缓冲液体系选自磷酸盐缓冲液或生理盐水。
- 根据权利要求8所述的一种基于等离子体浸没离子注入技术的高分子材料表面改性方法,其特征在于,所述处理过的高分子材料在含有生物分子的溶液中的孵育是指将具有生物活性的大分子溶解在磷酸盐缓冲液中,并在4℃条件下孵育12小时以上。
- 根据权利要求8所述的一种基于等离子体浸没离子注入技术的高分子材料表面改性方法,其特征在于,所述生物分子在等离子体浸没离子注入处理后的高分子材料表面的负载量通过孵育时所使用的生物溶液的浓度来调节,以及生物溶液的浓度为10-2000μg/mL之间。
- 根据权利要求8所述的一种基于等离子体浸没离子注入技术的高分子材料表面改性方法,其特征在于,通过在两种及以上生物分子的共混合溶液中孵育一段时间实现相应的生物分子在等离子体浸没离子注入处理后的高分子材料表面的同时负载。
- 一种表面以生物分子共价接枝修饰的高分子材料,其特征在于,使用上述任一项权利要求中的生物分子及高分子材料,且所述生物分子在高分子材料表面上的共价接枝根据相应上述任一项权利要求所述的方法制备。
- 权利要求13所述的一种表面以生物分子共价接枝修饰的高分子材料的用途,其特征在于:用于生物医用材料。
- 根据权利要求14所述的高分子材料的用途,其特征在于:用于医用植入材料、医用功能性材料、医用生物活性材料。
- 一种表面以生物分子共价接枝修饰的高分子材料,其特征在于,所述高分子材料选自能够经受等离子体浸没离子注入技术处理的高分子材料,优选聚四氟乙烯、聚醚醚酮;所述生物分子选自多糖、多肽、蛋白质、细胞因子,优选肝素、辣根过氧化物酶。
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