WO2018146943A1 - Aérateur et appareil de culture - Google Patents

Aérateur et appareil de culture Download PDF

Info

Publication number
WO2018146943A1
WO2018146943A1 PCT/JP2017/045282 JP2017045282W WO2018146943A1 WO 2018146943 A1 WO2018146943 A1 WO 2018146943A1 JP 2017045282 W JP2017045282 W JP 2017045282W WO 2018146943 A1 WO2018146943 A1 WO 2018146943A1
Authority
WO
WIPO (PCT)
Prior art keywords
sparger
culture
gas supply
culture vessel
supply pipe
Prior art date
Application number
PCT/JP2017/045282
Other languages
English (en)
Japanese (ja)
Inventor
貴史 浅倉
浩二 中村
博志 大川
Original Assignee
キリン株式会社
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by キリン株式会社 filed Critical キリン株式会社
Priority to JP2018566783A priority Critical patent/JP7220567B2/ja
Publication of WO2018146943A1 publication Critical patent/WO2018146943A1/fr

Links

Images

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M1/00—Apparatus for enzymology or microbiology
    • C12M1/04—Apparatus for enzymology or microbiology with gas introduction means
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M1/00—Apparatus for enzymology or microbiology
    • C12M1/04—Apparatus for enzymology or microbiology with gas introduction means
    • C12M1/08—Apparatus for enzymology or microbiology with gas introduction means with draft tube

Definitions

  • the present invention relates to a sparger and a culture apparatus using the same.
  • a culture tank containing a culture solution seeded with the cells and the like, and for aerating air to the culture solution in the culture tank A culture apparatus having a sparger and a stirring blade that stirs the culture solution in the culture tank is known (Patent Document 1, etc.).
  • the culture solution is agitated by mechanically moving the stirring blade in the culture tank to increase the chance of contact between the culture solution and cells or to promote cell dispersion.
  • the cells and the like may be damaged by contact between the cells and the stirring blade during the cultivation of the cells and the like.
  • cells that do not have a cell membrane like mesenchymal stem cells and are extremely weak against shearing force are greatly damaged by contact with a stirring blade and cannot be efficiently cultured. is there.
  • a lower portion of a pair of culture tubes is connected by a bent tube to form a U A culture tank formed in a letter shape, connected to the lower part of the left and right culture cylinders, gas blowing means for alternately supplying gas to the culture solution, connected to the upper part of the left and right culture cylinders, and supplied into the culture cylinder
  • gas blowing means for alternately supplying gas to the culture solution, connected to the upper part of the left and right culture cylinders, and supplied into the culture cylinder
  • the culture solution can be moved left and right in a U-shaped culture tank by alternately supplying gas to the left and right culture tubes, There is a problem that a sufficient agitation effect cannot be obtained from the viewpoint of increasing the chance of contact between the culture solution and cells and promoting cell dispersion.
  • the present invention provides a sparger that can provide a sufficient stirring effect by supplying gas to a culture solution and that does not cause splashing of the culture solution, and a culture apparatus using the same.
  • the purpose is to provide.
  • the present invention provides a sparger for introducing a gas into a culture vessel in the form of bubbles, the sparger main body at least partially comprising a porous body, and the culture vessel
  • the entire sparger body is preferably composed of the porous body, the average pore diameter of the porous body is preferably 20 to 50 ⁇ m, and the porous body is 20 to 50 ⁇ m. It is preferable to have pores with a pore size distribution of
  • the bottom surface of the bottomed hole is preferably positioned below the center of the sparger body in a state where the opening of the bottomed hole is positioned upward.
  • the main body is preferably substantially spherical, and the diameter of the sparger main body is preferably 10 to 20 mm.
  • the present invention also includes a culture vessel capable of containing a culture, a sparger according to the invention provided in the culture vessel, one end inserted into the bottomed hole, and the other end supplied with gas. And a sparger provided in the culture vessel so that the porous body faces the bottom of the culture vessel.
  • the flow rate of the gas supplied to the sparger through the gas supply pipe is preferably 300 to 700 mL / min, and the bottom of the culture vessel is preferably substantially hemispherical.
  • the present invention it is possible to provide a sparger that can obtain a sufficient stirring effect by supplying gas to the culture solution and does not cause the liquid splash of the culture solution, and a culture apparatus using the sparger. it can.
  • FIG. 1 is a side view showing a schematic configuration of a culture apparatus according to an embodiment of the present invention.
  • FIG. 2 is a side view showing a schematic configuration of the culture apparatus in one embodiment of the present invention.
  • FIG. 3A is a perspective view illustrating a schematic configuration of a cap according to an embodiment of the present invention.
  • FIG. 3B is a top view illustrating a schematic configuration of the cap according to the embodiment of the present invention.
  • FIG. 4 is a perspective view showing a schematic configuration of a sparger in one embodiment of the present invention.
  • FIG. 5 is a cross-sectional view showing a schematic configuration of a sparger in one embodiment of the present invention.
  • FIG. 6 is a side view for explaining the operational effects of the culture apparatus according to the embodiment of the present invention.
  • FIG. 1 is a side view illustrating a schematic configuration of a culture apparatus according to the present embodiment
  • FIG. 2 is a side view illustrating a schematic configuration of the culture apparatus according to the present embodiment
  • FIG. 3A is a cap according to the present embodiment
  • FIG. 3B is a top view showing the schematic configuration of the cap
  • FIG. 4 is a perspective view showing the schematic configuration of the sparger in the present embodiment
  • FIG. It is sectional drawing which shows schematic structure of the sparger in this embodiment
  • FIG. 6 is a side view for demonstrating the effect in the culture apparatus which concerns on this embodiment.
  • a culture apparatus 1 includes a culture container 2 that can accommodate a culture object, a sparger 3 provided in the culture container 2, and a gas supply pipe attached to the sparger 3. 4.
  • a gas supply unit (not shown) is connected to the gas supply tube 4, and gas (air) is supplied in the form of bubbles from the gas supply unit to the culture solution via the gas supply tube 4 and the sparger 3. it can.
  • Examples of the culture medium that can be cultured in the culture apparatus 1 according to the present embodiment include animal cells, plant bodies, plant tissues, plant cells, algae, fungi, yeasts, aerobic bacteria, and the like.
  • the culture solution 8 (see FIG. 6) that can be accommodated in the culture vessel 2 together with the culture object is suitable for the culture object.
  • Dulbecco's modified Eagle (DMEM) medium Roswell Park Memorial Institute ( (RPMI) 1640 medium, Murashige scoog (MS) medium, Gamborg B5 medium, Luria Bertani (LB) medium, YPD Broth (Yeast Extract-Peptone-Dextrose) medium, PD Broth (Potato Dextrose) medium and the like.
  • the culture vessel 2 is made of polyethylene terephthalate (PET), polybutylene terephthalate, polyethylene naphthalate, polyester resin such as polytrimethylene terephthalate, polytrimethylene naphthalate, olefin resin such as polyethylene or polypropylene, polycarbonate resin, polyacrylate resin. Further, it may be made of a resin material such as a polyamide resin, glass or the like.
  • the capacity of the culture vessel 2 is, for example, 500 mL to 200 L, preferably 1 L to 20 L.
  • the culture container 2 includes a mouth portion 21, a neck portion 22 that continues to the mouth portion 21, a shoulder portion 23 that continues to the neck portion 22, and a trunk portion 24 that continues to the lower end of the shoulder portion 23, And a bottom portion 25 continuous with the lower end of the body portion 24.
  • the mouth portion 21 has a substantially cylindrical shape, and a thread for attaching the cap 5 (see FIG. 3) is formed on the outer surface of the mouth portion 21 on the opening end side. Thereby, after accommodating to-be-cultured material and the culture solution 8 (refer FIG. 6) in the culture container 2, the culture container 2 can be sealed by attaching the cap 5 to the opening
  • the shoulder portion 23 is configured by a curved structure that curves from the continuous portion with the neck portion 22 toward the outside of the culture vessel 2 and gradually increases in diameter toward the trunk portion 24.
  • the bottom portion 25 has a convex curved shape toward the outside of the culture vessel 2, that is, a substantially hemispherical shape (so-called round bottom). Since the bottom 25 of the culture vessel 2 has a round bottom, the objects to be cultured that settle in the culture vessel 2 can be collected below the sparger 3, so that the culture is performed by gas (air) discharged from the sparger 3. By stirring the liquid 8, the culture object can be efficiently dispersed.
  • the cap 5 can be made of a resin material such as polyethylene or polypropylene, or a metal material such as aluminum.
  • the diameter (outer diameter) of the cap 5 can be appropriately set according to the size of the mouth portion 21 of the culture vessel 2, but is, for example, about 28 to 52 mm, and preferably about 28 to 38 mm.
  • the top surface 51 of the cap 5 has a first hole 52 through which the gas supply pipe 4 (see FIG. 1) can be inserted, and a culture solution into the culture vessel 2.
  • An exhaust filter 55 is provided inside the top surface 51 of the cap 5 so as to cover at least the third hole 54.
  • the sparger 3 provided in the culture vessel 2 is formed along a substantially spherical sparger body 31 and the radial direction of the sparger body 31, and the gas supply pipe 4 ( 1) and a bottomed hole 32 into which one end is inserted.
  • the opening 33 of the bottomed hole portion 32 of the sparger 3 is directed upward, it is sufficient that at least the lower half of the sparger body 31 is made of a porous body, and the entire sparger body 31 is made of a porous body. It may be constituted by.
  • the gas (air) is bubbled through the gas supply pipe 4 inserted into the bottomed hole 32 and the culture vessel 2 While being able to be introduced into the inside, bubbles are discharged from the sparger body 31 toward the bottom 25 of the culture vessel 2.
  • a flow of the culture solution 8 (see FIG. 6) is generated upward from the bottom of the culture vessel 2, and the culture solution 8 convects in the entire culture vessel 2. Therefore, the culture solution 8 in the culture vessel 2 can be agitated by supplying gas (air) from the sparger 3.
  • Examples of the material constituting the sparger body 31 include resin materials such as polyethylene, polypropylene, polymethyl methacrylate, polystyrene, and fluororesin, and ceramic materials such as alumina and SiC.
  • the porous body constituting at least the lower half or the whole of the sparger body 31 with the opening 33 of the bottomed hole portion 32 of the sparger body 31 facing upward has pores having an average pore diameter of 20 to 50 ⁇ m. It is particularly preferable to have pores having an average pore diameter of 20 to 30 ⁇ m. If the average pore diameter of the pores of the porous body is less than 20 ⁇ m, there is a risk of liquid splashing on the surface of the culture solution, and if it exceeds 50 ⁇ m, the effect of stirring the culture solution in the culture vessel 2 is insufficient. There is a risk of becoming.
  • the pores of the porous body constituting at least the lower half or the whole of the sparger body 31 preferably have random pore diameters within a predetermined pore diameter distribution range, and randomly within a pore diameter distribution range of 20 to 50 ⁇ m. It is more preferable to have a fine pore diameter. Since the pores in the porous body have random pore diameters within a predetermined pore diameter distribution range, particularly within a pore diameter distribution range of 20 to 50 ⁇ m, the stirring effect of the culture medium in the culture vessel 2 can be improved. .
  • the average pore size and pore size distribution in the porous body can be measured by a laser microscope (for example, VK-X200 (manufactured by Keyence Corporation)).
  • the bottomed hole portion 32 plays a role as a gas supply pipe connection portion to which the gas supply pipe 4 is connected, and has an inner diameter into which one end of the gas supply pipe 4 can be inserted.
  • the inside diameter of the tube 4 is such that it cannot be easily pulled out.
  • the bottom surface 34 of the bottomed hole portion 32 is positioned below the center C of the sparger body 31 in a state where the opening 33 of the bottomed hole portion 32 is positioned upward. Since the bottom surface 34 of the bottomed hole portion 32 is positioned below the center C of the sparger body 31, the gas supply tube 4 is made to have a bottomed hole so that one end of the gas supply tube 4 is brought into contact with the bottom surface 34.
  • one end of the gas supply pipe 4 can be positioned below the center of the sparger body 31.
  • gas air
  • the center C of the sparger body 31 means the center of gravity when the sparger body 31 is spherical.
  • the length D between the bottom surface 34 of the bottomed hole 32 and the center C of the sparger body 31 (the length in the radial direction of the sparger body 31) is 10 to 40% of the diameter of the sparger body 31. Can be set to a degree.
  • the inner diameter of the bottomed hole 32 can be appropriately set such that the gas supply pipe 4 can be inserted and the gas supply pipe 4 cannot be easily removed, and can be set to about 6 to 8 mm, for example.
  • the size of the sparger body 31 is not particularly limited, and can be set as appropriate according to the capacity (size) of the culture vessel 2.
  • the culture vessel 2 has a capacity of about 500 mL to 5000 mL.
  • the diameter of the sparger body 31 is preferably about 10 to 20 mm. If the diameter of the sparger body 31 is about 10 to 20 mm, the culture solution 8 can be sufficiently stirred by the gas (bubbles) discharged from the sparger body 31.
  • the position of the sparger 3 in the culture vessel 2 is not particularly limited.
  • the distance T from the bottom 25 of the culture vessel 2 to the lower end of the sparger 3 is determined by the amount of the object to be cultured that stays in the bottom 25 by gas (air) discharged from the sparger 3 toward the bottom 25 of the culture vessel 2 Any distance that can be dispersed in the culture solution 8 may be used.
  • the distance T is preferably about 5 mm or less, and more preferably about 0 to 3 mm. If the distance T exceeds 5 mm, culturing caused by bubbles discharged from the sparger 3 may cause the culture object to stay in the bottom 25 of the culture vessel 2 without being stirred.
  • the sparger 3 in this embodiment uses an upper mold and a lower mold having cavities in accordance with the shape of the sparger main body 31, and fills the cavities with resin particles having a predetermined particle diameter, and heats them. Then, it can be produced by partially fusing adjacent resin particles.
  • the sparger 3 composed of a porous body having a predetermined pore size distribution can be produced in the same manner as described above using resin particles having a predetermined particle size distribution.
  • the sparger 3 whose lower half is made of a porous body is formed by applying a resin material such as an epoxy resin to the upper half of the sparger body 31 manufactured as described above, and the upper half of the pores in the upper half. It can be made by filling with material.
  • the culture object and the culture solution are accommodated in the culture vessel 2, and gas (air) is supplied to the culture solution from the gas supply unit (not shown) via the gas supply pipe 4. Supply.
  • the gas (air) that has passed through the gas supply pipe 4 is discharged in the form of bubbles through the pores of the porous body of the sparger body 31.
  • the bottom surface 34 of the bottomed hole portion 32 of the sparger body 31 is positioned below the center of the sparger body 31 (on the bottom 25 side of the culture vessel 2), so that one end of the gas supply pipe 4 is It is located below the center of the sparger body 31.
  • the gas (air) that has passed through the gas supply pipe 4 is discharged from the porous body of the sparger body 31, but one end of the gas supply pipe 4 (that is, the gas discharge port) is the center C of the sparger body 31. If it is located above, the gas (air) discharged from the sparger body 31 (porous body) will be directed upward from the sparger body 31. However, as in the present embodiment, the gas (air) discharged from the sparger body 31 is positioned below the center C of the sparger body 31 so that the sparger is directed toward the bottom of the culture vessel 2.
  • the flow rate of the gas (air) supplied to the culture solution via the gas supply pipe 4 is preferably set to about 300 to 700 mL / min, more preferably about 400 to 500 mL / min. If the flow rate of the gas (air) is less than 300 mL / min, there is a possibility that at least a part of the culture object will stay in the bottom 25 of the culture vessel 2. If the flow rate exceeds 700 mL / min, the liquid of the culture solution Liquid splashes may occur on the surface, and the exhaust filter 55 may be contaminated.
  • a sufficient stirring effect can be obtained without increasing the amount of gas (air) supplied to the culture solution. It is possible to prevent the exhaust filter 55 from being contaminated without causing splashing or the like.
  • Example 1 A sparger 3 having a bottomed hole portion 32 having a diameter of 10 mm and an average pore diameter of 20 ⁇ m and having an inner diameter of 6 mm, a gas supply pipe 4, a culture vessel 2 having an internal volume of 3000 mL, and a cap 5 And the culture apparatus 1 having the structure shown in FIG. 1 was assembled.
  • Example 2 A gas supply tube 4, a culture vessel 2 and a cap 5 were prepared in the same manner as in Example 1 except that the sparger 3 was composed of a porous body having a diameter of 15 mm, and the culture device 1 was assembled.
  • Example 3 A gas supply tube 4, a culture vessel 2, and a cap 5 were prepared in the same manner as in Example 1 except that the sparger 3 was composed of a porous body having a diameter of 20 mm, and the culture device 1 was assembled.
  • Example 4 A gas supply tube 4, a culture vessel 2 and a cap 5 were prepared in the same manner as in Example 1 except that the sparger 3 was composed of a porous body having a diameter of 25 mm, and the culture device 1 was assembled.
  • Example 5 Example 1 except that an epoxy resin is applied to the surface of the upper half of the sparger body 31 with the opening of the bottomed hole portion 32 facing upward, and the pores of the upper half are filled with the epoxy resin.
  • a sparger 3, a gas supply pipe 4, a culture vessel 2 and a cap 5 were prepared, and the culture apparatus 1 was assembled.
  • Example 6 Example 2 except that an epoxy resin was applied to the upper half surface of the sparger body 31 with the bottomed hole 32 facing upward, and the upper half pores were filled with the epoxy resin.
  • a sparger 3, a gas supply pipe 4, a culture vessel 2 and a cap 5 were prepared, and the culture apparatus 1 was assembled.
  • Example 7 Example 3 except that the upper half surface of the sparger body 31 with the opening of the bottomed hole portion 32 facing upward is coated with epoxy resin and the upper half pores are filled with the epoxy resin.
  • a sparger 3, a gas supply pipe 4, a culture vessel 2 and a cap 5 were prepared, and the culture apparatus 1 was assembled.
  • Example 8 Example 4 except that an epoxy resin was applied to the surface of the upper half of the sparger body 31 with the opening of the bottomed hole portion 32 facing upward, and the pores of the upper half were filled with the epoxy resin.
  • a sparger 3, a gas supply pipe 4, a culture vessel 2 and a cap 5 were prepared, and the culture apparatus 1 was assembled.
  • Example 9 A gas supply tube 4, a culture vessel 2 and a cap 5 were prepared in the same manner as in Example 6 except that the sparger 3 was composed of a porous material having an average pore diameter of 50 ⁇ m, and the culture device 1 was assembled.
  • Example 10 A gas supply tube 4, a culture vessel 2 and a cap 5 were prepared in the same manner as in Example 7 except that the sparger 3 was composed of a porous material having an average pore diameter of 50 ⁇ m, and the culture device 1 was assembled.
  • Example 11 The gas supply tube 4, the culture vessel 2 and the cap 5 were the same as in Example 2 except that the sparger 3 was composed of a porous body having pores with random pore sizes in the pore size distribution range of 20 to 50 ⁇ m. And the culture apparatus 1 was assembled.
  • Example 12 The gas supply tube 4, the culture vessel 2, and the cap 5 are the same as in Example 3 except that the sparger 3 is composed of a porous material having pores with random pore sizes in the pore size distribution range of 20 to 50 ⁇ m. And the culture apparatus 1 was assembled.
  • Test Example 2 In the culture apparatus 1 of Examples 1 to 4, the gas supply pipe 4 was connected to the sparger 3 so that one end of the gas supply pipe 4 was located below the center of the sparger body 31, and Similarly, a test for evaluating the stirring efficiency was conducted. In Test Example 2, the gas flow rate was varied in the range of 300 to 900 mL / min, and the stirring efficiency at each flow rate was evaluated. The results are shown in Table 3.
  • Test Example 3 In the culture apparatuses 1 of Examples 1 to 12 and Comparative Examples 1 to 4, the gas supply pipe 4 is connected to the sparger 3 so that one end of the gas supply pipe 4 is located below the center of the sparger body 31. In the same manner as in Test Example 1, a test for evaluating the stirring efficiency was conducted. In Test Example 3, the gas flow rate was varied in the range of 300 to 700 mL / min, and the stirring efficiency at each flow rate was evaluated. The results are shown in Table 4.
  • Example 5 in which the lower half of the sparger body is made of a porous body, compared to Examples 1 to 4 in which the entire sparger body is made of a porous body. It was confirmed that ⁇ 8 was superior in stirring effect.
  • Examples 6 and 7 in which the average pore diameter of the porous body is relatively smaller than those in Examples 9 and 10 in which the average pore diameter of the porous body is relatively large are more effective for stirring. It was confirmed to be excellent.
  • Example 11 and Example 12 in which the entire sparger body is composed of a porous body, the pores of the porous body have random pore sizes within a predetermined pore size distribution range. As a result, turbulent flow can be generated in the culture solution in the culture vessel, and it was confirmed that the stirring effect was excellent.

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Microbiology (AREA)
  • Sustainable Development (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

Un aérateur pour introduire un gaz sous forme de bulles dans un récipient de culture est équipé d'un corps principal d'aérateur dont au moins une partie est formée à partir d'un corps poreux et d'une section de raccordement de tube d'alimentation en gaz à laquelle un tube d'alimentation en gaz pour fournir un gaz à introduire dans le récipient de culture jusqu'au corps principal d'aératateur est connecté, la section de raccordement du tube d'alimentation en gaz étant composée d'une section perforée d'extrémité fermée qui est formée dans le corps principal d'aérateur et à travers laquelle le tube d'alimentation en gaz peut être inséré, et au moins une moitié inférieure du corps principal d'aérateur est formée à partir d'un corps poreux lorsqu'une ouverture dans la section perforée d'extrémité fermée se présente.
PCT/JP2017/045282 2017-02-10 2017-12-18 Aérateur et appareil de culture WO2018146943A1 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP2018566783A JP7220567B2 (ja) 2017-02-10 2017-12-18 スパージャー及び培養装置

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2017-022951 2017-02-10
JP2017022951 2017-02-10

Publications (1)

Publication Number Publication Date
WO2018146943A1 true WO2018146943A1 (fr) 2018-08-16

Family

ID=63108110

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/JP2017/045282 WO2018146943A1 (fr) 2017-02-10 2017-12-18 Aérateur et appareil de culture

Country Status (2)

Country Link
JP (1) JP7220567B2 (fr)
WO (1) WO2018146943A1 (fr)

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH078264A (ja) * 1993-06-21 1995-01-13 Tokyo Gas Co Ltd バイオリアクター
JPH10201467A (ja) * 1997-01-20 1998-08-04 Ishikawajima Harima Heavy Ind Co Ltd 被培養体の生長判定方法および培養装置
JP2011256150A (ja) * 2010-06-11 2011-12-22 Taoka Chem Co Ltd ９−フルオレノン類の製造方法
JP2015524792A (ja) * 2012-06-26 2015-08-27 ジーイー・ヘルスケア・アクスイェ・セルスカプ 気体マイクロバブルを含む組成物の調製

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH078264A (ja) * 1993-06-21 1995-01-13 Tokyo Gas Co Ltd バイオリアクター
JPH10201467A (ja) * 1997-01-20 1998-08-04 Ishikawajima Harima Heavy Ind Co Ltd 被培養体の生長判定方法および培養装置
JP2011256150A (ja) * 2010-06-11 2011-12-22 Taoka Chem Co Ltd ９−フルオレノン類の製造方法
JP2015524792A (ja) * 2012-06-26 2015-08-27 ジーイー・ヘルスケア・アクスイェ・セルスカプ 気体マイクロバブルを含む組成物の調製

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
OKUMURA , TAISEI ET AL.: "Microbubble '' for Microbial Culture", JGC TECHNICAL JOURNAL, vol. 01, no. 01, 2011, pages 1 - 6 *
TANINO, TAKANORI ET AL.: "Surfactant Decomposition by Discharge Plasma in Microbubble Dispersed Water", JOURNAL OF THE INSTITUTE OF ELECTROSTATICS JAPAN, vol. 34, no. 1, 2010, pages 31 - 36 *

Also Published As

Publication number Publication date
JP7220567B2 (ja) 2023-02-10
JPWO2018146943A1 (ja) 2020-01-23

Similar Documents

Publication Publication Date Title
JP6892486B2 (ja) 培養方法
US10155924B2 (en) Multilayer tissue culture vessel
US20170226458A1 (en) Spheroid trap insert
US11767499B2 (en) Cell culture vessel
US20200181552A1 (en) Handling features for microcavity cell culture vessel
US9174181B2 (en) Disposable bioreactor for culturing cells in a nutrient medium
CN109312284B (zh) 用于培养细胞的减少叶轮摆动的容器和旋转烧瓶
US9499290B2 (en) Stationary bubble reactors
US7709251B2 (en) Method and apparatus for transferring growth media and infection fluids to a cell growth bag
WO2015156367A1 (fr) Recipient de culture cellulaire en forme de colonne octogonale
JP2016202180A (ja) 細胞培養器、及び、細胞培養システム
US7850365B1 (en) Toroidal convection mixing device
JP6827322B2 (ja) 生物学的存在の増殖のための容器
WO2018146943A1 (fr) Aérateur et appareil de culture
CN111615424A (zh) 微小气泡生成方法和微小气泡生成装置
JP3214876U (ja) 培養基材
US5879932A (en) Slow release microorganism dispenser
US6730509B2 (en) Controlled release dispenser
WO2019014610A1 (fr) Récipient de culture cellulaire pour une culture 3d et procédés de culture de cellules 3d
GB2301374A (en) Vessel for culturing plantlets
KR102541149B1 (ko) 초미세 기포를 생성하는 산기장치용 멤브레인
CN218811793U (zh) 一种悬滴培养板及悬滴培养装置
US20150132827A1 (en) Emulsifier Centrifuge Tube Fermenter
CN111989392A (zh) 生物反应器
KR20230010677A (ko) 궤도형 진탕 세포 배양, 특히 현탁 배양을 위한 생물반응기

Legal Events

Date Code Title Description
121 Ep: the epo has been informed by wipo that ep was designated in this application

Ref document number: 17896217

Country of ref document: EP

Kind code of ref document: A1

ENP Entry into the national phase

Ref document number: 2018566783

Country of ref document: JP

Kind code of ref document: A

NENP Non-entry into the national phase

Ref country code: DE

122 Ep: pct application non-entry in european phase

Ref document number: 17896217

Country of ref document: EP

Kind code of ref document: A1