WO2018105919A2 - Pomegranate beverage containing marine collagen and method for manufacturing same - Google Patents

Abstract

The present invention relates to a pomegranate beverage containing marine collagen and a method for manufacturing the same and, more particularly, to a beverage containing marine collagen extracted from fish by-products and pomegranates beneficial to health, and a method for manufacturing the same. The pomegranate beverage containing marine collagen of the present invention contains 1 to 10 parts by weight of a pomegranate concentrate, 0.5 to 5 parts by weight of marine collagen extracted from fish, 10 to 15 parts by weight of white sugar, 2 to 10 parts by weight of an oligosaccharide, 0.1 to 0.6 parts by weight of citric acid, and 0.05 to 0.5 parts by weight of vitamin C.

Description

Pomegranate drink containing marine collagen and preparation method thereof

The present invention relates to a pomegranate drink containing marine collagen and a method for producing the same, and a drink containing a marine collagen extracted from fish and a pomegranate useful for health and a method for producing the same.

Collagen is the most abundant protein in the animal body, accounting for more than 30% of body protein, and has been found to be at least 19 types (Type I-III) (Nakamura, YN et al., Relationship among collagen amount, distribution). and architecture in the M. Longissimus thoracis and M. pectoralis profundus from pigs.Meat Science, 64, pp 43-50, 2003). In addition, collagen is a major protein of animal connective tissue, and supports tissues and organs and surrounds the body surface and plays a role in maintaining body shape. In particular, as a major component of connective tissue such as skin, cartilage and bone in the living body, 40% is widely distributed throughout the body, such as skin, 20% bone and cartilage, and other blood vessels and internal organs.

Unlike myofibrillar proteins, which have a double helix structure, collagen has a triple helix structure, about 14-15 nm in diameter, 280-300 nm in length, and an average molecular weight of about 300 KDa (Lehninger, AL Biochemistry, 2nd ed., Pp. 145, 1975), have a regular sequence of amino acids such as (Gly-XY) n and share within or between tropocollagen molecules, the basic molecules of fibrous proteins. Physically and biologically stable structures are formed by binding crosslinking (McClain, PE et al., Amino acid composition and cross-linking characteristics of collagen intramuscular connective tissue of striated muscle (Bos taurus). of Biochemistry, 2 (7), pp 121-124, 1971).

Collagen has been applied as a raw material of leather or gelatin for a long time, and its field of application has become more diverse in recent years. In the food industry, it is used for meat packaging materials such as sausage and salami as an edible casing raw material. Collagen applied to medicines has been reported to have a healing effect on skin damaged by burns or wounds (Jeyanthi, R. et al., Solid tumour chemotherapy using implantable collagen-poly (HEMA) hydrogel containing 5-fluorouracil. of Pharmacy & Pharmacology, 43, pp. 60-62, 1991). As such, collagen is not only used as a functional material for foods and medicines, but also has a function of enhancing skin moisturizing, and thus is widely used in various fields such as basic materials of cosmetics.

Collagen hydrolyzate, called collagen peptide, refers to a polymer that has been extracted from donpi, and then hydrolyzed through post-treatment such as enzymatic digestion to reduce the molecular weight into peptides. Are sold.

Meanwhile, fishery by-products have been classified as food waste so far, and most of them have been used as compost. Dry skin has more than 90% of protein and useful substances, so it can be used in various ways such as collagen extraction, cosmetics, dietary supplements, and adhesives. Recently, various products such as health supplements and cosmetics using collagen are commercialized, but many depend on imports.

Increasing utilization of skin by-products in the context of Korea's per capita aquatic consumption with Japan, the world's number one, is highly suitable for economic synergies and eco-friendly industrial development through the supply of high quality fish protein and collagen.

In addition, it contains a large amount of various polymer proteins such as collagen, albumin, myosin, elastin, and can be utilized as high-quality collagen, and it is easy to collect in domestic where the consumption of sashimi is high. As marine fish farming is also being done consistently, stable yields can be expected. Therefore, there is an urgent need to evaluate the availability of fish by-products.

(Prior art document)

(Patent literature)

(Patent Document 1) Republic of Korea Patent No. 10-0775055: Health functional beverage containing collagen extracted from deer antler and its manufacturing method

The present invention has been made to improve the above problems, and an object thereof is to provide a beverage containing marine collagen extracted from fish and pomegranate useful for health, and a method for producing the same.

Pomegranate drink containing marine collagen of the present invention for achieving the above object is 1 to 10 parts by weight of pomegranate concentrate, 0.5 to 5 parts by weight of marine collagen extracted from fish, 10 to 15 white sugar based on 100 parts by weight of purified water It contains weight part, 2-10 weight part of oligosaccharides, 0.1-0.6 weight part of citric acid, and 0.05-0.5 weight part of vitamin C.

The pomegranate drink further contains 1 to 10 parts by weight of sageumchocho extract based on 100 parts by weight of the purified water.

And a method for producing a pomegranate drink containing marine collagen of the present invention for achieving the above object comprises a concentration step of obtaining a pomegranate concentrate by concentrating the pomegranate juice; A collagen extraction step of extracting marine collagen from fish; 1 to 10 parts by weight of the pomegranate concentrate, 0.5 to 5 parts by weight of marine collagen, 10 to 15 parts by weight of white sugar, 2 to 10 parts by weight of oligosaccharide, 0.1 to 0.6 parts of citric acid, Stirring the mixture containing the vitamin C 0.05 to 0.5 parts by weight; And a heating step of heating the mixture at 83 to 87 ° C. for 10 to 20 minutes after the stirring.

The collagen extraction step is a) adding a sodium hydroxide solution to the skin powder to obtain an alkali residue from which the non-collagenic protein has been removed, b) adding an acetic acid solution to the alkali residue and stirred and separating the supernatant with a centrifuge Extracting acid-solubilized collagen, c) adding pepsin to the acid-solubilized collagen, stirring, separating the supernatant with a centrifugal separator, and then adding the sodium chloride solution to dialysis the precipitate precipitated with distilled water to extract pepsin-solubilized collagen and and d) purifying the pepsin solubilized collagen.

In the stirring step, weedelia ( Wedelia) based on 100 parts by weight of the purified water prostrata ) 1 to 10 parts by weight of the extract is further combined and stirred.

The present invention as described above can provide a beverage containing a marine collagen extracted from fish and pomegranate useful for health and a preparation method thereof.

In addition, the present invention has the advantage that can be used as a useful resource by the waste skin by-products.

Hereinafter, with reference to the accompanying drawings will be described in detail a pomegranate drink containing marine collagen of the present invention and a method for producing the same.

Pomegranate drink according to an embodiment of the present invention is 1 to 10 parts by weight of pomegranate concentrate, 100 to 5 parts by weight of purified water, 0.5 to 5 parts by weight of marine collagen extracted from fish, 10 to 15 parts by weight of white sugar, 2 to 10 oligosaccharides It contains a weight part, 0.1-0.6 weight part of citric acid, and 0.05-0.5 weight part of vitamin C.

Pomegranate drink according to another embodiment of the present invention further contains the sageumchocho. For example, 1 to 10 parts by weight of pomegranate concentrate, 0.5 to 5 parts by weight of marine collagen extracted from fish, 1 to 10 parts by weight of seaweed fireweed extract, 10 to 15 parts by weight of white sugar, oligosaccharide 2 To 10 parts by weight, citric acid 0.1 to 0.6 parts by weight, and vitamin C 0.05 to 0.5 parts by weight.

Pomegranate drink of the present invention is well known food additives may be added. Examples of food additives include sugars such as monosaccharides, disaccharides, polysaccharides, and sugar alcohols, flavoring agents such as taumartin, stevia extract, saccharin, and aspartame, nutrients, vitamins, edible electrolytes, flavors, coloring agents, and enhancers (e.g., cheese). , Chocolate, etc.), pectic acid, alginic acid, organic acid, protective colloid thickener, pH adjuster, stabilizer, preservative, glycerin, alcohol, carbonation agent and the like can be used.

The method for producing the pomegranate drink described above will be described.

Method for producing a pomegranate beverage according to an embodiment of the present invention is a concentration step of obtaining a pomegranate concentrate by concentrating the pomegranate juice, a collagen extraction step of extracting marine collagen from fish, and the pomegranate concentrate 1 with respect to 100 parts by weight of purified water To 10 parts by weight, 0.5 to 5 parts by weight of the marine collagen, 10 to 15 parts by weight of white sugar, 2 to 10 parts by weight of oligosaccharide, 0.1 to 0.6 parts by weight of citric acid, and 0.05 to 0.5 parts by weight of vitamin C And a stirring step of stirring, and a heating step of heating the mixture at 83 to 87 ° C. for 10 to 20 minutes after stirring, and packaging the mixture after heating. Look specifically at each step.

1. Concentration step

Pomegranate juice is concentrated to obtain a pomegranate concentrate.

Pomegranate juice is obtained by juice of the pomegranate fruit. And pomegranate juice is concentrated using a concentrator to obtain a pomegranate concentrate. For example, the pomegranate juice may be concentrated to a concentration of about 50 to 80 Brix using a rotary vacuum concentrator to obtain a pomegranate concentrate.

2. Collagen Extraction Stage

Next, marine collagen is extracted from fish, a byproduct of fish.

Fish skin can be used as a shell of fish such as halibut, shellfish, sea bass, red snapper, salmon and the like.

The collagen extraction step is a) adding sodium hydroxide solution to the skin powder to obtain an alkali residue from which the non-collagenic protein has been removed, b) adding an acetic acid solution to the alkali residue, stirring and separating the supernatant with a centrifuge Extracting solubilized collagen, c) adding pepsin to the acid-solubilized collagen, stirring and separating the supernatant with a centrifuge, and then extracting pepsin solubilized collagen by dialysis of the precipitate precipitated by adding sodium chloride solution; d) purifying the pepsin solubilized collagen.

Skin is washed, dried and then pulverized to a size of 50 to 150 mesh to prepare the skin powder. Predrying may be performed to remove scales on the skin prior to drying. To do this, the washed fish is mixed with 0.1 M sodium hydroxide solution and stirred for 6 to 12 hours. When the mixture is stirred with sodium hydroxide solution, there are some differences depending on the type of fish, but 80 to 90% of scales are easily removed.

The fish powder and sodium hydroxide solution were mixed at a weight ratio of 1: 5 to 10, and then stirred at room temperature (20 to 25 ° C.) for 12 to 24 hours to obtain an alkali residue from which the non-collagenic protein was removed using a centrifuge. can do.

The alkaline residue is washed with distilled water, and then acetic acid is added to extract collagen. Alkaline residue and acetic acid solution are mixed at a weight ratio of 1: 5 to 10, and then stirred at room temperature (20 to 25 ° C) for 12 to 24 hours, and the supernatant is separated using a centrifuge to obtain acid solubilized collagen.

Then, pepsin was added to the acid-solubilized collagen, stirred for 10 to 20 hours, the supernatant was separated by centrifugation, and the precipitate precipitated by adding 2 M sodium chloride solution was dialyzed with distilled water to obtain pepsin solubilized collagen.

In order to purify pepsin solubilized collagen to obtain marine collagen, pepsin solubilized collagen was dialyzed at 20 mM Na ₂ HPO ₄ to inactivate pepsin and then dialyzed against 50 mM acetic acid solution containing urea (pH 4.8), followed by ion chromatography. Fractions are used to elute. For example, purification was performed with a linear gradient (60 ml / h) of 0-600 mM NaCl in a column filled with cellulose phosphate (P11, Whatman, Maidstone, UK), and the fraction eluted at 230 nm was 0.5 containing 2.0 M NaCl. Dialysis with M acetate solution is recovered, followed by dialysis and freeze-drying with distilled water to obtain high purity marine collagen.

Meanwhile, instead of extracting marine collagen by the above-described method, a commercially available powdered marine collagen product may be purchased and used. In this case, the process of extracting marine collagen is of course omitted.

3. Stirring Step

Next, pomegranate concentrate, marine collagen, additives and the like are mixed with purified water and stirred.

For example, 1 to 10 parts by weight of pomegranate concentrate, 0.5 to 5 parts by weight of marine collagen, 10 to 15 parts by weight of white sugar, 2 to 10 parts by weight of oligosaccharide, 0.1 to 0.6 parts of citric acid, , 0.05 to 0.5 parts by weight of vitamin C is blended. The mixture so blended is stirred so that the whole is uniformly mixed.

And well known food additives may be further added in the stirring step. Examples of food additives include sugars such as monosaccharides, disaccharides, polysaccharides, and sugar alcohols, flavoring agents such as taumartin, stevia extract, saccharin, and aspartame, nutrients, vitamins, edible electrolytes, flavors, coloring agents, and enhancers (e.g., cheese). , Chocolate, etc.), pectic acid, alginic acid, organic acid, protective colloid thickener, pH adjuster, stabilizer, preservative, glycerin, alcohol, carbonation agent and the like can be used.

On the other hand, another example of the present invention may be further mixed by mixing 1 to 10 parts by weight of jinbul fireweed extract in the stirring step. For example, 1 to 10 parts by weight of pomegranate concentrate, 0.5 to 5 parts by weight of marine collagen extracted from fish, 1 to 10 parts by weight of seaweed fireweed extract, 10 to 15 parts by weight of white sugar, oligosaccharide 2 To 10 parts by weight, citric acid 0.1 to 0.6 parts by weight, and vitamin C 0.05 to 0.5 parts by weight.

Wedelia prostrata is a perennial herb that grows on the beach, and young sprouts are eaten in the form of herbs. Mudweed uses leaves.

Various extracting methods can be applied to obtain the sageumchocho extract. It can be obtained by adding an extraction solvent as one of the extraction methods. At least one selected from water, a lower alcohol having 1 to 4 carbon atoms, a polyhydric alcohol, or a mixture thereof may be used as the extraction solvent. Methanol, ethanol, etc. can be used as a C1-C4 lower alcohol, Butylene glycol, propylene glycol, pentylene glycol, etc. can be used as a polyhydric alcohol. And the mixture may be a mixture of water and lower alcohol, a mixture of water and polyhydric alcohol, a mixture of lower alcohol and polyhydric alcohol, or a mixture of water and lower alcohol and polyhydric alcohol.

As an example of the extraction, after adding 2 to 20 times the extraction solvent in the weight ratio with respect to the loose leaf buds can be used for hot water extraction, cold needle or hot needle extraction for 10 to 150 ℃ for 1 to 48 hours. After extraction, the resultant is filtered to obtain liquid mudweed.

Saenggeum fireweed is a salty plant that contains a variety of minerals and can increase the functionality of pomegranate drink. In addition, sageumchocho extract is excellent in antioxidant activity, increasing the long-term preservation of pomegranate drink and is useful for health.

4. Heating step

After stirring the mixture is heat treated at 83-87 ° C. for 10-20 minutes. The heat treatment makes the mixture evenly and sterilized at the same time.

5. Packing step

After heating, the mixture can be packed by packing the packaging. Pouches, spout pouches, and PET can be used for packaging to maintain simplicity and quality in the distribution process.

Hereinafter, the present invention will be described through examples. However, the following examples are only for illustrating the present invention in detail, and the scope of the present invention is not limited to the following examples.

(Example 1)

Pomegranate juice obtained by juice of the pomegranate fruit was concentrated to a concentration of about 60 Brix using a rotary vacuum concentrator to prepare a pomegranate concentrate. Marine collagen was prepared by purchasing commercialized powder products (fish collagen, geltec, Korea).

10 parts by weight of pomegranate concentrate, 2 parts by weight of marine collagen, 12 parts by weight of white sugar, 5 parts by weight of oligosaccharides, 0.3 parts by weight of citric acid, and 0.2 parts by weight of vitamin C were added. Pomegranate drink was prepared by heating at 15 ° C.

(Example 2)

Pomegranate concentrate and marine collagen were prepared in the same manner as in Example 1.

And 10 parts by weight of pomegranate concentrate, 2 parts by weight of marine collagen, 8 parts by weight of seaweed fireweed extract, 12 parts by weight of white sugar, 5 parts by weight of oligosaccharide, 0.3 parts by weight of citric acid, and vitamin After blending 0.2 parts by weight of C, heat treatment was performed at 85 ° C. for 15 minutes to prepare a pomegranate drink.

The sageumchocho extract was added 10 times by weight of water to the sageumcho leaf and then extracted with hot water at 90 ° C. for 8 hours and then filtered through a filter paper to use as a liquid extract.

Antioxidant Experiment

Antioxidant activity was tested using the fermented beverages of Examples 1 and 2 as samples.

0.5 mL of 0.2 mM DPPH (1,1-diphenyl-2-picryl hydrazyl) was added to 2 mL of the sample, stirred for 30 minutes, and then absorbance was measured at 517 nm. The radical scavenging ability was expressed as the absorbance decrease rate of the sample addition group and the no addition group. Radical decay capacity was calculated by the following equation.

Radical scavenging ability (%) = (absorbance of 1-sample addition / absorption of no addition) x 100

The results of measuring the radical scavenging ability are shown in Table 2 below.

division	Radical scavenging ability
Example 1	40.7 ± 0.5
Example 2	65.1 ± 0.3

Referring to the results of Table 1, the radical scavenging ability of Example 2 was much higher than that of Example 1. This seems to be due to the antioxidant effect of sageumchocho.

Therefore, the pomegranate drink added with the lotus leaf bud is expected to be useful for preventing the aging and preventing various diseases when it is ingested as a food.

As described above, the present invention has been described with reference to one embodiment, which is merely exemplary, and those skilled in the art will understand that various modifications and equivalent embodiments are possible therefrom.

Claims (5)

1. 1 to 10 parts by weight of pomegranate concentrate, 0.5 to 5 parts by weight of marine collagen extracted from fish, 10 to 15 parts by weight of white sugar, 2 to 10 parts by weight of oligosaccharide, and 0.1 to 0.6 parts by weight of citric acid And, pomegranate drink containing marine collagen, characterized in that it contains 0.05 to 0.5 parts by weight of vitamin C.
2. The pomegranate drink containing marine collagen according to claim 1, wherein the pomegranate drink further contains 1 to 10 parts by weight of a sageweed extract, based on 100 parts by weight of the purified water.
3. Concentrating the pomegranate juice to obtain a pomegranate concentrate;

   A collagen extraction step of extracting marine collagen from fish;

   1 to 10 parts by weight of the pomegranate concentrate, 0.5 to 5 parts by weight of marine collagen, 10 to 15 parts by weight of white sugar, 2 to 10 parts by weight of oligosaccharide, 0.1 to 0.6 parts of citric acid, 0.05 to 0.5 parts by weight of vitamin C is blended and stirred;

   Method for producing a pomegranate drink containing marine collagen, characterized in that it comprises a; heating step of heating the mixture for 10 to 20 minutes at 83 to 87 ℃ after stirring.
4. The method of claim 3, wherein the collagen extraction step is a) adding sodium hydroxide solution to the skin powder to obtain an alkali residue from which the non-collagenic protein has been removed, and b) adding an acetic acid solution to the alkali residue, followed by centrifugation. Separating supernatant with a separator and extracting acid solubilized collagen; c) adding pepsin to the acid-solubilized collagen and stirring, separating the supernatant with a centrifugal separator, and adding precipitated sodium chloride solution to dialyzine with distilled water to solubilize pepsin. Extracting collagen, and d) purifying the pepsin solubilized collagen.
5. The method of claim 3, wherein the stirring step further comprises mixing 1 to 10 parts by weight of kaleidocho extract with respect to 100 parts by weight of the purified water, and mixing the marine collagen-containing pomegranate drink.