WO2018105799A1 - Composition cosmétique contenant un extrait d'eichhornia crassipes utilisé comme principe actif - Google Patents

Composition cosmétique contenant un extrait d'eichhornia crassipes utilisé comme principe actif Download PDF

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Publication number
WO2018105799A1
WO2018105799A1 PCT/KR2016/014621 KR2016014621W WO2018105799A1 WO 2018105799 A1 WO2018105799 A1 WO 2018105799A1 KR 2016014621 W KR2016014621 W KR 2016014621W WO 2018105799 A1 WO2018105799 A1 WO 2018105799A1
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Prior art keywords
extract
cosmetic composition
cream
skin
active ingredient
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PCT/KR2016/014621
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English (en)
Korean (ko)
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안인숙
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주식회사 진셀팜
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Priority to CN201680091346.4A priority Critical patent/CN110022854B/zh
Publication of WO2018105799A1 publication Critical patent/WO2018105799A1/fr

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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
    • A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
    • A61K8/9783—Angiosperms [Magnoliophyta]
    • A61K8/9794—Liliopsida [monocotyledons]
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
    • A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q19/00—Preparations for care of the skin
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q19/00—Preparations for care of the skin
    • A61Q19/08—Anti-ageing preparations
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
    • A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
    • A61K2800/85—Products or compounds obtained by fermentation, e.g. yoghurt, beer, wine

Definitions

  • the present invention relates to a cosmetic composition containing the water hyacinth extract as an active ingredient.
  • Fine dust is an air pollutant containing harmful substances such as sulfurous acid gas, nitrogen oxides, ozone, and carbon monoxide, which occurs in automobiles and factories, and refers to a material that floats in the air for a long time.
  • Fine dust is invisible to small dust of less than 10 ⁇ m diameter, it is known that contains toxic substances such as sulfate, nitrate and the like. These fine dusts are produced by photochemical reactions of toxic substances or heavy metals from automobile fumes and factory chimneys in the air.In case of exposure to high concentrations of fine dust environment, cough, eye irritation, skin trouble, and inflammation of lung and airway cells May cause.
  • fine dust weakens the metabolism of the skin and lowers sebum control function may worsen itching and dryness. Impurities in the skin may not be properly removed, causing problems, and the fat balance on the skin surface may be disrupted, resulting in dryness and itching. In addition, fine dust may promote skin aging. The absorbed fine dust stimulates the pigment cells of the skin, causing black mushrooms and wrinkles.
  • Fine dust can be removed to some extent by cleansing, but the finer it is, the stronger the adsorptive force, so deeply penetrates into the pores can not be completely removed by normal cleansing.
  • an object of the present invention is to include a natural material as an active ingredient is excellent in biosafety, in particular, it provides a cosmetic composition excellent in fine dust adsorption power, including a water hyacinth extract It is.
  • a cosmetic composition for adsorption of fine dust comprising a water hyacinth extract as an active ingredient.
  • the water hyacinth extract may be a fermentation extract prepared by inoculating a fermentation strain.
  • the fermentation strain may be Lactobacillus kimchicus .
  • the extract is water, anhydrous or hydrous lower alcohol having 1 to 4 carbon atoms, acetone, petroleum ether, ethyl acetate, butyl acetate, trichloromethane, dichloromethane, chloroform, hexane and 1,3-butylene It may be extracted with one or more solvents selected from the group consisting of glycols.
  • the alcohol may be 65 to 75% concentration (v / v) of ethanol.
  • the cosmetic composition is composed of flexible cosmetics, nourishing cosmetics, moisture cream, nutrition cream, massage cream, essence, ampoule, gel, eye cream, cleansing cream, cleansing foam, cleansing water, pack, spray and powder It may be formulated into one or more selected from the group.
  • Eichhornia Provided is a cosmetic composition for antioxidant, anti-inflammatory, skin moisturizing, or anti-wrinkle comprising crassipes ) extract as an active ingredient.
  • the cosmetic composition includes a hyacinth extract, that is, an extract derived from a natural plant as an active ingredient, the skin improvement effect is excellent, and in particular, the wrinkle improvement and the adsorption and removal effect of fine dust may be remarkably improved. .
  • the numerical range includes the numerical values defined in the range. All maximum numerical limits given throughout this specification include all lower numerical limits as if the lower numerical limits were clearly written. All minimum numerical limits given throughout this specification include all higher numerical limitations as if the higher numerical limit were clearly written. All numerical limitations given throughout this specification will include all better numerical ranges within the broader numerical range, as the narrower numerical limitations are clearly written.
  • a cosmetic composition comprising the hyacinth extract as an active ingredient.
  • the cosmetic composition may include an extract of natural origin as an active ingredient, as well as excellent skin improvement effect and biosafety, and may minimize skin irritation.
  • the cosmetic composition is excellent in antioxidant, anti-inflammatory, skin moisturizing effect, as well as skin wrinkle improvement and fine dust adsorption due to various active ingredients contained in water hyacinth.
  • Eichhornia ” crassipes ) is an aquatic plant of the Aquaticaceae, which can be harvested year round in the tropical and subtropical regions, and is an attribute plant that can be harvested more than eight times a year if the water temperature is maintained at 20 degrees or higher. It has the ability to absorb and remove nitrogen and phosphorus, which are the causes of underwater eutrophication, at 1,700 kg / ha and 300 kg / ha. Since the water hyacinth contains a variety of effective ingredients beneficial to a variety of skin can be used for the purpose of improving the skin and has an excellent effect of adsorbing fine dust in the air.
  • extract refers to a solvent in which the active ingredient contained in the extract is transferred by contacting the solvent and the extract raw material under specific conditions. All may be included regardless. For example, extracting a component dissolved in a solvent from a natural product using water or an organic solvent, and may include both a specific component of the natural product, such as obtained by extracting only a specific component such as oil.
  • the mixed extract may be extracted with one or more solvents selected from the group consisting of purified water, lower alcohol having 1 to 4 carbon atoms, acetone, ethyl acetate, butyl acetate, and 1,3-butylene glycol, wherein the alcohol is 65 to 75% concentration (v / v) of ethanol.
  • solvents selected from the group consisting of purified water, lower alcohol having 1 to 4 carbon atoms, acetone, ethyl acetate, butyl acetate, and 1,3-butylene glycol, wherein the alcohol is 65 to 75% concentration (v / v) of ethanol.
  • the active ingredient contained in the raw material may be different in the extraction ratio according to the polarity of the solvent, the ethanol has excellent selectivity in the extraction of physiologically active substances of the natural raw material to achieve the optimal skin improvement effect by the ethanol extraction Can be.
  • Water and ethanol are different in polarity, the active ingredient extracted according to each polarity can be different, and the concentration of the ethanol can be appropriately controlled so that the optimum skin improvement effect can be implemented. At this time, if the concentration of the ethanol is more than 75% may not be achieved a proper yield, if less than 65% may not be sufficiently extracted the effective ingredient showing the skin improvement effect.
  • the extract is washed with water, washed with water, dried and pulverized, and extracted with conventional methods such as reflux circulation extraction, pressure extraction, and ultrasonic extraction for about 1 to 24 hours with a solvent having a volume of 8 to 12 times the weight of the raw material. And may be prepared by filtration. In addition, the extract may be obtained in a powder state by an additional process such as distillation under reduced pressure or freeze drying.
  • the extract may also include an extract that has undergone conventional purification.
  • the extract may be subjected to various purification methods additionally performed, such as separation using an ultrafiltration membrane having a constant molecular weight cut-off value, separation by various chromatography (manufactured for separation according to size, charge, hydrophobicity, or affinity). It may comprise a fraction obtained through.
  • the cosmetic composition may include a fermentation extract prepared by inoculating a fermentation strain in the water hyacinth extract.
  • transformation refers to a process in which a specific microorganism uses an enzyme to decompose organic matter.
  • Raw materials that have undergone fermentation may have a significantly improved skin improvement effect than before fermentation due to changes in the properties of the active ingredient.
  • Metabolites contain various amino acids, organic acids, and antioxidants that are beneficial to the skin by fermentation, thereby promoting skin metabolism and imparting elasticity to the skin texture.
  • the active ingredient particles may be reduced in size by fermentation and decompose toxins such as heavy metals, thereby reducing side effects such as skin troubles and allergies and improving absorption.
  • the “fermented extract” may be prepared by inoculating fermented strains after the hyacinth extract is dried naturally or using a rotary pressure reducer and a lyophilizer, diluted to a certain concentration in a specific solvent.
  • the fermentation strain is Lactobacillus genus (Lactobacillus sp.), Pseudomonas in coarse (Monascus sp.), Bacterial genus bifidobacteria (Bifidobacterium sp . ), Prevotella sp . , Fusobacterium sp . ), And Eubacterium sp . Strain, and may be selected from the group consisting of the strains of the genus Lactobacillus.
  • the strains of the genus Lactobacillus Lactobacillus ( Lactobacillus kimchicus ), Lactobacillus pentosus ( Lactobacillus pentosus ), Lactobacillus brevis ( Lactobacillus brevis ), Lactobacillus plantarum ( Lactobacillus plantarum ), Lactobacillus casei (L) Or Lactobacillus acidophilus ( Lactobacillus acidophilus ), but may be preferably Lactobacillus kimchicus ( Lactobacillus kimchicus ).
  • the cosmetic composition is one or more selected from the group consisting of flexible cosmetics, nourishing cosmetics, moisture cream, nutrition cream, massage cream, essence, ampoule, gel, eye cream, cleansing cream, cleansing foam, cleansing water, pack, spray and powder Can be formulated.
  • the cosmetic composition may be for wrinkle improvement or fine dust adsorption, supple cosmetics, astringent cosmetics, nourishing cosmetics, lotions, nourishing cream, massage cream, essence, eye cream, cleansing cream, cleansing foam, cleansing water, pack, spray, And it may be formulated with one or more selected from the group consisting of a powder.
  • the cosmetic composition may be appropriately blended with other ingredients within the range of not impairing the object according to the present invention in accordance with the type or purpose of use of the formulation other than the active ingredient in each formulation.
  • the cosmetic composition may be prepared according to the quality or function of the final product, such as fatty substances, organic solvents, solubilizers, thickeners, gelling agents, softeners, antioxidants, suspending agents, stabilizers, foaming agents, fragrances, surfactants, water, Commonly used in cosmetics or dermatology, such as ionic or nonionic emulsifiers, fillers, metal ion rod blockers, chelating agents, preservatives, blockers, wetting agents, essential oils, dyes, pigments, and hydrophilic or lipophilic active agents Supplement may be additionally included.
  • ionic or nonionic emulsifiers such as ionic or nonionic emulsifiers, fillers, metal ion rod blockers, chelating agents, preservatives, blockers, wetting agents, essential oils, dyes, pigments, and hydrophilic or lipophilic active agents Supplement may be additionally included.
  • the adjuvant and its mixing ratio are preferably selected appropriately so as not to affect the desirable properties of the cosmetic composition according to the present invention.
  • the water was washed with hyacinth, dried at room temperature and pulverized to obtain 100 g of coarse pulverized product.
  • 100 g of each pulverized product was immersed in 10-fold volume of 70% concentration (v / v) ethanol and extracted under reflux for 12 hours at a temperature of 80 ° C.
  • the extract was filtered with 250 mesh and 0.5 ⁇ m filter paper and extracted three times in the same manner for the remaining raw materials and then cooled at room temperature.
  • the extract was concentrated under reduced pressure at 20 ° C. and lyophilized to give a solid.
  • Bifidobacterium bifiderum Bifidobacterium bifidum
  • the fermented extract was filtered with 500 mesh and 0.2 ⁇ m filter paper to obtain a hyacinth fermented extract and used as a sample of Example 2.
  • Example 3 A sample of Example 3 was obtained in the same manner as in Example 2, except that Lactobacillus plantarum was used as the fermentation strain.
  • Example 4 A sample of Example 4 was obtained in the same manner as in Example 2 except for using Lactobacillus kimchicus as a fermentation strain.
  • Example 1 The extracts of Example 1 were suspended at concentrations of 0, 20, 40, 60, 80, and 100 ug / mL, and the fermentation extracts of Examples 2 to 4 were prepared at concentrations of 0.001, 0.005, 0.01, 0.05, and 0.1% ( w / w), respectively.
  • Cytotoxicity was performed by modifying Mosmann's method of measuring cell viability using MTT ⁇ 3- (4,5-dimethylthiazol-2-yl) -2-5-diphenyltetrazolium bromide ⁇ reagent.
  • Fibroblasts (HDF), melanocytes (B16F10), and keratinocytes (HaCaT) were each dispensed in 96-well plates at a concentration of 1 ⁇ 10 4 cells / well and incubated at 37 ° C., 5% CO 2 for 48 hours. It was.
  • MTT was dissolved in PBS at 5 mg / mL, 50 L was added, and incubated for 48 hours at 37 ° C. and 5% CO 2 . 100 L of DMSO (dimethyl sulfoxide) was added per well, stirred for 10 minutes, and the absorbance was measured at 540 nm.
  • DMSO dimethyl sulfoxide
  • the free radical scavenging activity was measured using the free radical DPPH (1, 1-diphenyl-2-picryl hydrazyl).
  • the extract was evaluated to have higher free radical scavenging activity than ⁇ -tocopherol and BHT, which are well known in the art. Therefore, the decomposition of free radicals may be promoted by the extract, and the results suggest that skin aging and skin condition improvement effect by the antioxidant activity is excellent.
  • Raw 264.7 cells were incubated in 96 well plates at a concentration of 1 ⁇ 10 4 cells / well. The medium was exchanged with fresh medium and the cells were incubated for 24 hours after treatment of the extract.
  • the extract was diluted with dimethylsulfoxide (DMSO, Sigma-Aldrich, USA) to a maximum concentration of 20 ⁇ M based on the final concentration, DMSO was used as a blank test (negative control). The experiment was repeated three times to secure the reliability.
  • DMSO dimethylsulfoxide
  • the experiment was performed under the same cell line and the same cell culture conditions as the WST-1 assay, a cell activity measurement test.
  • the cultured Raw 264.7 cells were washed once with a solution of Phosphate Buffered Saline (PBS; sodium chloride 137 mM, phosphate buffer 10 mM, potassium chloride 2.7 mM, all from Biopure, Canada) and then harvested Raw 264.7 cells. Suspended in 15 mL PBS solution, 15 ⁇ L of 20 mM 2 ', 7'-Dichlorofluorescein diacetate was added and incubated for 1 hour.
  • PBS Phosphate Buffered Saline
  • the amount of reactive oxygen species was evaluated by measuring the fluorescence at 485 nm excitation / 535 nm emission using a fluorescence microplate reader.
  • the extract treated in the reactive oxygen species measurement test for the sample concentration was used as the sample concentration set by measuring the cell activity.
  • DMSO DMSO was used instead of the sample material.
  • ascorbic acid Sigma-Aldrich
  • DMSO was diluted to 100 mg / mL in DMSO and used at a concentration of 100 ⁇ g / mL.
  • Example 1 0 48.2 61.5
  • Example 2 0 51.3 65.8
  • Example 3 0 53.7 67.4
  • Example 4 0 57.2 70.1
  • the extract was evaluated to equal levels of ascorbic acid and free radical scavenging activity well known in the art. That is, the results suggest that the active oxygen species removal ability of Examples 1 to 4 is excellent, and the antioxidant effect due to free radical scavenging activity is excellent.
  • NF-kB transcription inhibition effect was measured by NF-kB reporter assay.
  • INOS, COX-2, IFN- ⁇ , TNF- ⁇ , etc. induced by LPS are expressed by the transcription factor NF-kB.
  • the NF-kB is inactivated in complex with IkB in the cytoplasm.
  • I-kB- ⁇ is phosphorylated by I-kB- ⁇ kinase
  • the bound complex is decomposed and activated by NF-kB to move into the nucleus.
  • )do Induces expression of target gene of NF-kB and causes inflammatory action.
  • TRIF which acts on inflammatory diseases such as multiple sclerosis, is a TIP-domain with an interferon- ⁇ -inducing receptor, which is a receptor that responds to the activity of TLRs (toll-like-receptors). It recognizes the specific composition of activating the immune response to the antigen. The receptor recognizes well-conserved pathogenic patterns and activates signals to stimulate the release of inflammatory cytokines.
  • IRF-3 interferon stimulatory gene 3
  • IRF-3 is a transcription activating factor induced by interferon ⁇ / ⁇ stimulation and binds to the interferon stimulation sequence (ISRE) in the transcriptional regulatory region of interferon-induced genes and activates its transcription.
  • NF-kB depending on the presence of receptor molecules (MyD88 and TRIF), such as ⁇ -galactosidase, using the PEI method in 12-well plates according to the manufacturer's protocol (Jin et al., 2009).
  • 1 ⁇ g of plasmid containing each of Luc or TRIF was transfected into HEK293 (1 ⁇ 10 6 cells / ml) cells. After culturing for 24 hours, LPS (100 ng / ml) and the extract were treated, and after 24 hours, luciferase assays were used to measure NF-kB activity and IRF-3 activity.
  • the luciferase assay was performed using a luciferase assay system (Promega Co., USA) reported in the manufacturer's protocol (Jung et al., 2009), and the analysis results are shown in Table 3 below.
  • the treatment of the extract significantly reduced the activity of NF-kB and IRF-3.
  • the results suggest that the extract effectively inhibits the transcription of NF-kB, thereby inhibiting or alleviating inflammation and preventing and improving inflammatory diseases.
  • the anti-inflammatory activity of the extract was evaluated by analyzing the expression levels of TNF- ⁇ , iNOS, and COX-2.
  • RNA was prepared from LPS treated RAW264.7 cells. The method was prepared using Trizol Reagent (Gibco BRL) as described by Lee et al., 2008. All RNA was used after storage at -70 °C. Semi-quantitative RT reaction was performed using MuLV reverse transcriptase.
  • RNA (1 ⁇ g) was incubated with oligo-dT15 at 70 ° C. for 5 minutes and mixed with 5 ⁇ first strand buffer, 10 mM dNTPs and 0.1 M DTT. The reaction mixture was further incubated at 37 ° C. for 5 minutes and incubated for 60 minutes after the addition of MuLV reverse transcriptase (2 U). The reaction was terminated by incubating at 70 ° C. for 10 minutes. RNase H was added to remove all RNA and PCR reactions were performed (conditions: 2 ⁇ L cDNA, 4 ⁇ M 5 ′ and 3 ′ primers, 10 ⁇ buffer (10 mM Tris-HCl, pH 8.3; 50 mM KCl and 0.1).
  • PCR reaction was denatured at 94 ° C. for 30 seconds and after annealing at 55 to 60 ° C. for 30 seconds, 72 It superposed
  • MRNA expression level measurement results according to the extract treatment is shown in Table 5.
  • MRNA analysis of RAW264.7 cells resulted in increased expression of TNF- ⁇ , iNOS, and COX-2 by the addition of LPS.
  • the expression of TNF- ⁇ , iNOS, and COX-2 induced by LPS decreased in a concentration-dependent manner when the extract was treated.
  • the sample powders obtained in Examples 1 to 4 were suspended in purified water, diluted by concentration, and the skin moisturizing effect was evaluated.
  • the expression level of the HAS2 gene and AQP3 gene involved in skin moisturization in human keratinocytes was measured at the mRNA level.
  • HaCaT Human keratinocytes
  • Keratinocytes were dispensed into 96 well plates at a concentration of 1 ⁇ 10 6 cells / mL and incubated for 24 hours. After exchange with DMEM free medium was treated extracts of Examples 1 to 4 diluted by concentration and incubated for 24 hours.
  • RNA extracted RNA was extracted.
  • PBS cold phosphate buffer
  • Trizol TM reagent Trizol TM reagent, Life Technologies, Inc.
  • Gene expression changes were measured by qRT-PCR (quantitative real time PCR), which binds the fluorescent material to the DNA product amplified in the polymerase chain reaction (PCR) and continuously detects the fluorescent material.
  • qRT-PCR quantitative real time PCR
  • fluorescence values emitted by PCR products were measured through SYBR green I (Invitrogen).
  • a reaction solution was prepared by mixing 0.2 ⁇ M primer, 50 mM KCl, 20 mM Tris / HCl pH 8.4, 0.8 mM dNTP, 0.5U Extaq DNA polymerase, 3 mM MgCl 2 , and 1 ⁇ SYBR green.
  • the Ct value is the number of cycles when the amount of fluorescence generated by the PCR product (the number of amplified PCR products) reaches a predetermined reference value above the basic value. Primer sequences used in the experiment are shown in Table 6 below.
  • HAS2 and AQP3 mRNA expression level measurement results are shown in Table 7 below, mRNA expression level was increased concentration-dependently according to the treatment of the extract. The higher the HAS2 and AQP3 expression promoting ability can be evaluated as an excellent skin moisturizing effect.
  • Example 1 0 ⁇ g / mL 1.00 1.00 50 ⁇ g / mL 1.23 1.07 100 ⁇ g / mL 1.86 1.24
  • Example 2 0 ⁇ g / mL 1.00 1.00 50 ⁇ g / mL 1.47 1.16 100 ⁇ g / mL 2.21 1.37
  • Example 3 0 ⁇ g / mL 1.00 1.00 50 ⁇ g / mL 1.61 1.26 100 ⁇ g / mL 2.43 1.48
  • Example 4 0 ⁇ g / mL 1.00 1.00 50 ⁇ g / mL 1.76 1.35 100 ⁇ g / mL 2.86 1.54
  • Human dermal fibroblasts were dispensed in 96 well plates at a concentration of 1.0 ⁇ 10 4 cells / mL, and cultured for 3 days at 37 ° C., 5% CO 2 , and humidified conditions.
  • the medium was exchanged with DMEM (Dulbecco's Modified Eagle's Medium, manufactured by Sigma), and incubated after treating the extracts 1 to 4 at a concentration of 50 mg / L. Purified water was added without treating the extract as a control.
  • DMEM Dulbecco's Modified Eagle's Medium, manufactured by Sigma
  • the culture medium was collected, and the concentration of type I collagen secreted in the culture medium was quantified by an enzyme-linked immunoassay (Procollagen type I c-peptide EIA Kit; manufactured by Takara Bio Co., Ltd.).
  • the amount of collagen in each test sample culture was calculated based on the type I collagen amount of the control group (100%), and the measurement results are shown in Table 8 below.
  • the sample powders obtained in Examples 1 to 4 were suspended in purified water, diluted by concentration, and the effect of improving wrinkles was evaluated.
  • the extracts of Examples 1 to 4 diluted by concentration were treated to human dermal fibroblasts and cultured for 24 hours to determine the degree of collagen expression change.
  • Gene expression changes were measured by qRT-PCR (quantitative real time PCR), which binds the fluorescent material to the DNA product amplified in the polymerase chain reaction (PCR) and continuously detects the fluorescent material.
  • qRT-PCR quantitative real time PCR
  • the Ct value is the number of cycles when the amount of fluorescence generated by the PCR product (the number of amplified PCR products) reaches a predetermined reference value above the basic value. Primer sequences used in the experiment are shown in Table 9 below.
  • the extract can promote the production of collagen and thereby improve the skin elasticity and wrinkles bar use of skin care It can be used as
  • Example 1 0% (w / w) 1.00 1.00 0.01% (w / w) 1.82 0.79 0.1% (w / w) 2.62 0.45
  • Example 2 0% (w / w) 1.00 1.00 0.01% (w / w) 1.89 0.77 0.1% (w / w) 2.95 0.42
  • Example 3 0% (w / w) 1.00 1.00 0.01% (w / w) 2.07 0.79 0.1% (w / w) 3.09 0.40
  • Example 4 0% (w / w) 1.00 1.00 0.01% (w / w) 1.95 0.72 0.1% (w / w) 3.16 0.37
  • the lotion formulated with extracts of Examples 1 to 4 was applied to 50 women aged 40 to 60 years every morning and evening twice a month for 1 month to evaluate the degree of wrinkle improvement.
  • the cream formulated with the extracts of Examples 1 to 4 significantly reduced the total number of wrinkles, wrinkle area, wrinkle length, skin wrinkle parameters, the result is that the extract improves skin wrinkles and skin beauty It can be used as a purpose.
  • the measured value was expressed as the average of three times except the maximum value and minimum value of Ra. The higher the measured value means a lot of skin wrinkles, the results are shown in Table 12.
  • the cream containing the hyacinth lactobacillus kimchicus fermented extract (Example 4) has the most excellent skin texture improvement effect, when the cream of the control group does not contain the hyacinth extract, the skin texture improvement effect is significantly insufficient. It was.
  • PAH polycyclic aromatic hydrocarbons
  • Examples 1 to 4 extracts were treated with the specimens at a concentration of 50 mg / L and reacted for 3 hours, and the control group was coated with PBS and reacted for 3 hours.
  • Example 1 Example 2
  • Example 3 Example 4 naphthalene 7.1 5.5 4.7 3.4 Acenaphthylene 10.5 8.2 6.4 5.1 Fluorene 9.3 7.3 5.1 2.8 Fluoranthene 9.3 6.9 4.5 3.2 Pyren 3.6 2.5 1.9 1.8 Krissen 4.9 3.9 2.3 1.3 Benzopyrene 3.1 2.7 2.2 1.3

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Abstract

La présente invention concerne une composition cosmétique contenant un extrait d'Eichhornia crassipes utilisé comme principe actif pour l'adsorption de poussière fine.
PCT/KR2016/014621 2016-12-08 2016-12-14 Composition cosmétique contenant un extrait d'eichhornia crassipes utilisé comme principe actif WO2018105799A1 (fr)

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CN201680091346.4A CN110022854B (zh) 2016-12-08 2016-12-14 包含凤眼蓝提取物作为有效成分的化妆品组合物

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KR1020160167061A KR101917740B1 (ko) 2016-12-08 2016-12-08 부레옥잠 추출물을 유효성분으로 함유하는 화장료 조성물

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KR102248056B1 (ko) * 2018-11-28 2021-05-03 황준환 조롱박 추출물을 함유하는 항산화용 화장료 조성물 및 그 제조방법
KR102085513B1 (ko) 2019-02-11 2020-03-05 주식회사 래디안 바질씨앗 추출물을 포함하는 화장료 조성물, 이를 이용한 건식 마스크 팩 및 그 제조방법
KR102277577B1 (ko) * 2019-11-07 2021-07-15 제주대학교 산학협력단 푸코스테롤을 포함하는 미세먼지에 대한 피부 보호용 조성물

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