WO2018101710A1 - Extrait de biomasse de palmier à huile en phase solide contenant une concentration élevée de substance physiologiquement active soluble dans les matières grasses produites à partir des sous-produits de production d'huile de palme et son procédé de production - Google Patents

Extrait de biomasse de palmier à huile en phase solide contenant une concentration élevée de substance physiologiquement active soluble dans les matières grasses produites à partir des sous-produits de production d'huile de palme et son procédé de production Download PDF

Info

Publication number
WO2018101710A1
WO2018101710A1 PCT/KR2017/013717 KR2017013717W WO2018101710A1 WO 2018101710 A1 WO2018101710 A1 WO 2018101710A1 KR 2017013717 W KR2017013717 W KR 2017013717W WO 2018101710 A1 WO2018101710 A1 WO 2018101710A1
Authority
WO
WIPO (PCT)
Prior art keywords
biomass
palm
oil
extract
fat
Prior art date
Application number
PCT/KR2017/013717
Other languages
English (en)
Korean (ko)
Inventor
유주현
배재한
Original Assignee
한국화학연구원
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 한국화학연구원 filed Critical 한국화학연구원
Publication of WO2018101710A1 publication Critical patent/WO2018101710A1/fr

Links

Images

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
    • C07C403/00—Derivatives of cyclohexane or of a cyclohexene or of cyclohexadiene, having a side-chain containing an acyclic unsaturated part of at least four carbon atoms, this part being directly attached to the cyclohexane or cyclohexene or cyclohexadiene rings, e.g. vitamin A, beta-carotene, beta-ionone
    • C07C403/24—Derivatives of cyclohexane or of a cyclohexene or of cyclohexadiene, having a side-chain containing an acyclic unsaturated part of at least four carbon atoms, this part being directly attached to the cyclohexane or cyclohexene or cyclohexadiene rings, e.g. vitamin A, beta-carotene, beta-ionone having side-chains substituted by six-membered non-aromatic rings, e.g. beta-carotene
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D17/00—Separation of liquids, not provided for elsewhere, e.g. by thermal diffusion
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D21/00—Separation of suspended solid particles from liquids by sedimentation

Definitions

  • the present invention is a method for extracting / recovering fat-soluble bioactive substances carotenoids, tocopherols and tocotrienols from the by-products or wastes left after the production of palm oil, and oil palm bio-containing high concentrations of fat-soluble bioactive substances such as carotenoids produced by the method. It relates to mass solid extract.
  • the fat-soluble physiological activity by solvent extraction including 10 to 30% by weight palm oil containing the fat-soluble bioactive substance, 15 to 40% by weight of solvent-insoluble oil palm biomass and 40 to 70% by weight of water
  • the present invention relates to an oil palm biomass solid extract and a method for preparing the same that can be used for industrial production of materials.
  • palm oil produced from oil palm fruit is composed of saturated fatty acid and unsaturated fatty acid in a ratio of 1: 1, so it shows semi-solid state at room temperature like animal oil or fat.
  • Unrefined palm oil is called crude palm oil (CPO), and 500 to 1,600 mg / kg carotenoids, 600 to 1,000 mg / kg tocopherol and tocotrienol, coenzyme Q10, It contains high value-added bioactive substances such as phytosterol and is red by beta-carotene.
  • EFB Empty fruit bunch of oil palm
  • Palm pressed fiber which is entangled like cobwebs in palm fruits containing palm oil and is released during the extraction process, accounts for 15% of palm fruits and contains a large amount of palm oil. Along with the room, it is used as boiler fuel for palm oil manufacturing plants.
  • This palm pulp fiber contains 5 to 7% by weight of palm oil, 4,000 to 6,000 mg / kg of carotenoids and 2,400 to 3,500 mg / kg of tocols, which are known to contain more bioactive substances than palm oil (Choo etc., JAOCS, 1996, 73, 599-602 .).
  • the palm fruit mill waste water (POME, palm oil mill) generated in the process of recovering palm oil by squeezing the nut and separating the liquid from the palm nut by steaming fresh fruit bunch of oil palm with high temperature steam.
  • effluent contains 95-96% by weight of water, 0.6-0.7% by weight of fat, and 4-5% by weight of solids. Protein, carbohydrates, fats and minerals are present in high concentrations. It can be used for the production of useful substances, but most of them are disposed of in wastewater treatment plants or used as raw materials for biogas production. It also contains a significant amount of palm oil, which contains a large amount of bioactive substances (about 1,500 mg / kg for carotenoids, Ahmad et al., Desalination Water Treat , 2009, 7, 251-256.).
  • palm oil production process by-products contain a significant amount of fat-soluble components, and these components also include high value-added bioactive substances such as beta carotene, alpha carotene, tocopherol and tocotrienol.
  • Beta-carotene has an antioxidant activity, has provitamin A activity, and has the characteristics of inhibiting the occurrence of various cancers such as pancreatic cancer, rectal cancer, prostate cancer, breast cancer, melanoma and skin cancer and preventing cataracts.
  • Alpha-carotene also has a cancer preventive effect and antioxidant activity and its activity is superior to beta-carotene, but provitamin A activity represents half of beta-carotene.
  • Tocopherol is an antioxidant that has vitamin E activity.
  • tocopherol When used in conjunction with other antioxidants, tocopherol prevents macular degeneration and prevents some cancers, cataracts, and Parkinson's disease. It is also used as a food preservative. Tocotrienols are more efficient antioxidants than tocopherols and have vitamin E activity, inhibit cancer formation and inhibit cholesterol formation.
  • bioactive materials are high value-added compounds present in a large amount in the by-products of palm oil production, but most of them are not recovered.
  • fat-soluble substances including such beta-carotene, alpha-carotene, tocopherol, tocotrienol are extracted from palm oil, but the amount is extremely limited.
  • beta-carotene is produced by chemical synthesis, which is less effective than natural substances, but the manufacturing cost is known to be 2,000 US dollars per kg.
  • these fat-soluble bioactive substances can be extracted inexpensively from palm oil manufacturing by-products, it is expected that their added value as natural bioactive substances will be more than synthetic materials.
  • the solvent-based extraction of fat-soluble physiologically active substances contained in such a large amount of biomass at low concentrations is 0.1 g / cm 3 for the bulk density of the biomass. Due to the excessive cost is inevitable, such as the use of an organic solvent and a large extraction equipment more than five times the weight of the biomass weight, more practical extraction methods are required.
  • the fat-soluble physiologically active substance contained in the palm oil by-product is dissolved in palm oil, and this palm oil has the characteristic of emulsifying and eluting in water when friction-pulverizing with water.
  • emulsion particles containing this fat-soluble physiologically active substance are attached to the biomass fine particles by coagulant, and if necessary, additional flocculant improves sedimentation by coarsening, thereby increasing dehydration efficiency.
  • the solid extract prepared in this way can be easily handled because it contains only a 1% of the volume of biomass while containing a high concentration of fat-soluble physiologically active substances of palm by-products.
  • the bioactive substance can be extracted at a high concentration even with a small amount of solvent, it is useful for the industrial production of high value-added bioactive substances contained in palm by-products.
  • the present invention was developed to solve the above problems, and aims to provide a simpler and more economical method for extracting a fat-soluble physiologically active substance containing a low concentration due to the small weight or excessive water content of palm oil production by-products. It is done.
  • oil palm biomass solid phase extract solidifying emulsion particles containing fat-soluble physiologically active substances that can be easily handled with only about 1% of the volume of biomass while containing a high concentration of fat-soluble physiologically active substances such as carotenoids, and a preparation method thereof.
  • the present invention provides solvent extraction including 10 to 30% by weight of palm oil containing fat-soluble physiologically active substance, 15 to 40% by weight of solvent-insoluble oil palm biomass and 40 to 70% by weight of water. It provides an oil palm biomass solid extract that can be used in the industrial production of fat-soluble physiologically active substances.
  • the biomass gruel manufacturing step of manufacturing a biomass gruel containing an emulsion by immersing the byproduct oil by-products in water and friction grinding; Liquid extract manufacturing step of preparing a liquid extract containing biomass fine particles and emulsion particles by solid-liquid separation of the biomass gruel obtained in the biomass gruel manufacturing step; Coagulant, flocculant or coagulant and flocculant are sequentially added to solidify palm oil emulsion particles containing fat-soluble bioactive substances in the liquid extract obtained from the liquid extract preparation step.
  • the oil palm biomass solid extract solidifying emulsion particles containing fat soluble bioactive substances according to the present invention can be easily handled because it contains only about 1% of the volume of biomass while containing a high concentration of fat soluble bioactive substances which palm by-products have. have.
  • the bioactive substance can be extracted at a high concentration even with a small amount of solvent, it is useful for the industrial production of high value-added bioactive substances contained in palm by-products.
  • the present invention enables to prepare a solid extract containing a high concentration of high value-added fat-soluble physiologically active substances such as beta-carotene from palm fruits, palm pulp fiber and palm oil plant wastewater, using as a raw material a small amount of organic solvent or supercritical extraction It is useful for industrially preparing fat-soluble physiologically active substances by utilizing various conventional extraction techniques and purification techniques such as chromatography.
  • 1 is a process chart showing the manufacturing step of the oil palm biomass solid extract according to an embodiment of the present invention.
  • lipophilic bioactive substance is a biochemical that is hardly soluble in water and soluble in organic solvents.
  • Carotenoids such as alpha carotene, beta carotene and gamma carotene, tocopherol and tocotrienol It refers to bioactive substances contained in palm fruits, palm pulp fiber and palm oil plant wastewater such as tocols.
  • carotenoid or “beta carotene” refers to a carotenoid and a beta form of the substance as a generic meaning containing various carotenes, respectively, but refers to other fat-soluble bioactive substances such as tocols. It is also used as a symbolic meaning.
  • biomass gruel refers to a biomass suspension prepared by adding water to biomass and friction grinding, and containing palm oil emulsion particles and biomass powder containing fat-soluble physiologically active substances. This biomass gruel is used as a raw material for primary solid-liquid separation, which separates palm oil emulsions and some fine biomass from most insoluble biomass.
  • liquid extract refers to a liquid substance in which most of the biomass is removed by solid-liquid separation of the biomass contaminants and palm oil emulsion particles and some biomass particles remaining in water.
  • Coagulant, flocculant or coagulant and flocculant is a target material to be aggregated by sequential addition.
  • coagulant refers to a substance used to adsorb / condense suspended particles to biomass particles in a liquid extract, and is a monomolecular inorganic coagulant, a high molecular weight inorganic coagulant, and a low molecular weight.
  • the organic polymer (molecular weight of 1 million or less) of the, the mono-molecular inorganic coagulant is aluminum sulfate (aluminum sulfate), ferrous sulfate (ferrous sulfate, ferric sulfate), ferric chloride (ferric chloride) and the like are representative, polymer type inorganic condensing agents include polyaluminum chloride (poly aluminum chloride: [Al 2 (OH) n Cl 6 -n] m), polyaluminum sulfate (poly aluminum sulfate: [Al 2 (OH) n (SO 4) 3 - n / 2 ] m ) and the like, and low molecular weight organic polymers include polydicyandiamide, polyamine, and polydiallydimethyldimethylammonium chl.
  • the coagulant according to the present invention may be in the form of a metal salt or any organic polymer, it is preferable to use an organic polymer in consideration of subsequent processes such as extraction of a fat-soluble physiologically active substance, and in particular, the organic polymer coagulant has a cationic property. It is preferable to use a polymer.
  • the coagulant according to the present invention serves to adsorb / condense the suspended particles to the biomass particles in the liquid extract.
  • floculant is a flocculant in order to more effectively perform the solidification of the adsorption / flocculation of the emulsion particles and biomass particles formed by the use of a coagulant in the solid-liquid separation step and solid extract form. It is a generic term for materials used to improve sedimentation and increase dehydration efficiency by coarsening, and is a polymer material having a molecular weight of 300 to 5 million or more, and is a nonionic polymer by homopolymerization mainly based on an acrylamide monomer. Anionic polymers prepared by copolymerization with phosphorus polyacrylamide, acrylate (acrylate) monomers or hydrolysis of acrylamide, etc.
  • the flocculant according to the present invention is very effective in coagulating adsorption / coagulum of emulsion particles and biomass particles to improve sedimentation and increase dehydration efficiency to solidify the solid extract form.
  • coagulant and "flocculant” are defined separately, but only coagulant or coagulant may be used, or more effective when both coagulant and flocculant are used.
  • the "solid extract” refers to palm oil, insoluble (solvent-insoluble) oil palm biomass, which is mostly produced when water is separated and solid-liquid separated by adding a polymer flocculant to the liquid extract. It means a solid material containing fine particles and a small amount of water.
  • Palm oil production by-products to extract the fat-soluble physiologically active substances in the present invention are palm fruit bunch (oil palm bunch), palm pressed fiber (palm pressed fiber) and palm oil mill effluent (palm oil mill effluent). Palm fruits contain both fresh fruit bunches at high temperature, dropping off palm fruits and then squeezed to recover the palm oil. do. These palm fruits and palm pulp fibers contain more physiologically active substances such as carotenoids, tocols and polyphenols, but are reduced rapidly as they decay due to the hot and humid environment of the mountain area, so it is desirable to dry them as soon as they are discharged from the factory. Do. Palm oil plant wastewater contains various water-soluble substances such as biomass, such as palm mesocarp in the form of fine particles, palm oil and protein in the emulsion state, and can be used for extraction of the bioactive substance as it is after being cooled to room temperature.
  • biomass such as palm mesocarp in the form of fine particles, palm oil and protein in the emulsion state
  • a coagulant or flocculant which serves to facilitate dehydration through filtration by adsorbing / coagulating / aggregating the palm oil in the oil state and the palm biomass in the particulate state is polydicyandiamide and polyacrylamide. It is preferable to use a cationic, anionic or nonionic polymer flocculant comprising a), and other coagulants or flocculants as described above may be added according to the type of biomass and the ratio of the emulsion, and are not limited thereto. Do not.
  • the solid extract containing a high concentration of a fat-soluble physiologically active substance prepared from the palm oil preparation by-product provided in the present invention is obtained as a solid after separation of the palm oil containing the fat-soluble physiologically active substance to biomass particles insoluble in water.
  • This solid extract consists of palm oil containing 40 to 70% water, 15 to 40% insoluble biomass and 15 to 30% bioactive material. It may also contain a small amount of coagulant or flocculant added during the preparation of the aggregate.
  • This solid extract contains bioactive substances such as alpha-carotene, beta-carotene, gamma-carotene, lycopene, tocopherol, tocotrienol and vegetable sterol, depending on the by-products of palm oil used, and the content of each bioactive substance depends on the type and composition of the raw materials. It is very different accordingly, and is not specifically limited. For example, when the fresh palm pulp fiber is used as a raw material, the concentration of the fat-soluble physiologically active substance of the solid extract is 2 to 5 times higher than that of the raw material, but the solid extract is 1/5 to 1 / by weight containing about 50% water. Decreases to 20 Among these solid extracts, insoluble in water is a biomass of cellulose main components derived from palm fruits, palm pulp or palm pulp.
  • the present invention is a.
  • biomass gruel manufacturing step of producing biomass grits containing emulsion by immersion by adding water to palm fruits or palm pulp, which is a by-product of palm oil, and by friction grinding;
  • Coagulant, flocculant or coagulant and flocculant are sequentially added to solidify palm oil emulsion particles containing fat-soluble bioactive substances in the liquid extract obtained from the liquid extract preparation step.
  • the fat-soluble physiologically active substance from palm oil manufacturing by-products includes a solid phase extracting step of preparing a solid extract including high concentrations of carotenoids and tocols through solid-liquid separation after the flocculation step but reducing the water content to about 40 to 70%. It provides a method for producing an oil palm biomass solid extract, characterized in that it contains a high concentration.
  • the friction grinding of the biomass grind production step may be to use the emulsification eluting principle of palm oil from the oil palm biomass.
  • the trituration of the biomass grind production step may use a twin screw extruder to extract the fat-soluble physiologically active material from the oil palm biomass.
  • the solid-liquid separation in the liquid extract manufacturing step may use a centrifugal separation method or a filtration method.
  • the step of producing the aggregates using at least one of a cationic, anionic or nonionic polymer flocculant including polydicyandiamide and polyacrylamide as a polymer flocculant It may be.
  • the solid-liquid separation in the solid phase extract manufacturing step may be to use a pressure filtration method including a filter press or a belt press.
  • the first step in the method for preparing a solid extract containing a high concentration of carotenoids and tocols from the palm oil production by-products according to the present invention is to add water to the palm oil production by-products and to prepare biomass grits through frictional grinding.
  • the palm bin or palm pulp fiber crushed is first subjected to 1 to 5 times more water than biomass drying and friction grinding.
  • the friction mill can be used is a twin screw extruder (disc mill) or disc mill (disc mill), in order to reduce the energy consumption and at the same time maximize the friction effect is preferably a twin screw extruder.
  • the palm biomass is crushed in water, and the palm oil contained in the biomass is eluted as emulsified particles by emulsification of glycolipids or phospholipids.
  • the efficiency of emulsifying and eluting palm oil may vary depending on the friction grinding conditions. When considering the properties of semi-solid palm oil at room temperature, the frictional grinding temperature is 20 o C or more and 100 o C or less, and the frictional grinding temperature is 20 to 50 o when additionally considering the carotenoid properties that may be altered at high temperature. C is preferred.
  • the operating conditions of the twin screw extruder or the disk mill are not limited to a specific range because they may vary depending on the type of equipment, the combination of screws or the type of the disk.
  • the second step of the present invention is the solid-liquid separation to prepare an emulsion containing the fat-soluble physiologically active component from the biomass gruel prepared in the first step and a liquid extract containing a small amount of biomass particles.
  • water is further added to the biomass gruel to contain 10 to 20 times more water than during drying to prepare a uniform gruel.
  • the solid-liquid separation of this gruel can be used a variety of methods commonly used in the art, and examples thereof include centrifugation, rotary dehydration, suction filtration and pressure filtration.
  • centrifugal separation using palm oil-containing emulsion particles having a specific gravity smaller than that of water is preferable. That is, some of the biomass particulates (eg, about 1% of the total biomass drying, etc.) are centrifuged at low speed so as to stay in the liquid phase (eg, 1 minute at 200 ⁇ g) and then fine biomass particles
  • the supernatant containing the emulsion can be separated from most of the biomass as a precipitate.
  • both filter press, belt press and centrifugal filtration can be used to separate and recover the emulsion and fine biomass from most biomass.
  • filter press or belt press with high energy recovery and high recovery of liquids can be used for solid-liquid separation.
  • the filter press having the highest recovery rate of the liquid is most preferred.
  • the third step of the present invention is to prepare a solid extract by adsorbing and dehydrating a small amount of biomass microparticles containing an emulsion containing a bioactive material.
  • the liquid extract obtained in the solid-liquid separation of the second step is treated with a polymer coagulant to coagulate the biomass fine particles and the emulsion particles, and thereafter, the coagulant is further treated to coagulate into particles that are hard enough to be filtered under pressure.
  • the polymer coagulant is made into a high concentration aqueous solution, and is added to the liquid phase and stirred while adsorbing to the suspended particles and the fine biomass.
  • the flocculant is then added and stirred and allowed to stand for at least several minutes so that aggregates can form.
  • the polymer coagulant and flocculant cationic, anionic and nonionic polymer coagulant including polydicyandiamide and polyacrylamide may be used, and solid phase extract may be prepared by solid-liquid separation.
  • the type and amount used are not limited as long as the aggregates are as hard as possible.
  • the pressurized filtration which is the lowest the water content of the solid extract obtained as a result of the solid-liquid separation is preferred, and it is more preferable to apply a filter press in terms of low energy consumption and low water content of the solid extract.
  • the solid extract containing high concentration of the bioactive substance prepared in this way is only 2 to 15% of the weight of the raw material biomass when compared to drying, and the water content is low to 40 to 70% by weight, so that a small amount of water-soluble organic solvent or supercritical water, Supercritical carbon dioxide can also extract economically soluble bioactive substances.
  • the present invention is 10 to 30% by weight of palm oil containing a fat-soluble bioactive substance such as carotenoids and tocol produced according to the above method; It provides an oil palm biomass solid phase extract that can be used for the industrial preparation of fat-soluble bioactive substances by solvent extraction, including 15 to 40% by weight of solvent-insoluble oil palm biomass and 40 to 70% by weight of water. .
  • the fat-soluble physiologically active substance may be a carotenoid or tocol, and at least one selected from the group consisting of alpha carotene, beta carotene, gamma carotene, lycopene, cotoperol and tocotrienol. Can be.
  • the oil palm biomass palm oil empty fruit bunch of oil palm
  • palm pressed fiber palm pressed fiber
  • palm oil plant generated as a by-product in the palm oil manufacturing process It may be at least one of palm oil mill effluent.
  • Solid extract containing a high concentration of the fat-soluble physiologically active substance of the present invention prepared as described above may then be used to extract carotenoids and tocol using a solvent. That is, the solvent was added at a ratio of 1: 1 (w / v) to 1:10 (w / v) with respect to the solid extract prepared by reducing the water content in step 3, followed by stirring to extract the bioactive material, followed by centrifugation or Filtration yields a solution containing carotenoids and tocols.
  • the solvent used to extract the fat-soluble physiologically active substance may include a water-soluble organic solvent, a poorly water-soluble organic solvent, supercritical water or supercritical carbon dioxide, and selected in consideration of the solubility and cost of the bioactive substance to be extracted.
  • Examples include water-soluble organic solvents such as alcohols such as ethanol, methanol and isopropyl alcohol, other organic solvents such as tetrahydrofuran, and poorly water-soluble organic solvents such as chloroform, dichloromethane, ethyl acetate, hexane, and other organic solvents.
  • water-soluble organic solvents such as alcohols such as ethanol, methanol and isopropyl alcohol
  • other organic solvents such as tetrahydrofuran
  • poorly water-soluble organic solvents such as chloroform, dichloromethane, ethyl acetate, hexane, and other organic solvents.
  • Supercritical water or supercritical carbon dioxide may be used in combination with the solvent, and all solvents and extraction methods available in the art may be used.
  • the small amount of solid extract containing high concentrations of carotenoids and tocol prepared from palm oil by-products according to the present invention enables economic production of industrial raw materials through solvent extraction and purification of physiologically active substances.
  • the solid extract was prepared by solid-liquid separation using a small filter press (Korea Institute of Chemical Research, Korea) attached to the 6 micron filter cloth. The solid extract was weighed, taken in small amounts and lyophilized to determine the moisture content. In addition, 2 g of this solid extract was taken in a 50 ml falcon tube, followed by ultrasonic extraction with 9 ml of ethanol, followed by centrifugation to prepare a supernatant. Carotenoids were quantified as indicators of fat-soluble bioactive substances by a spectrophotometer (Benchmark Plus microplate spectrophotometer, BIO RAD, USA, 450 nm).
  • the ethanol remaining in the falcon tube was decanted and blown with air to remove the ethanol by volatilization.
  • 50 ml of hexane (n-hexane) was added thereto, followed by ultrasonic extraction, centrifugation and supernatant removed. Air was blown to remove hexane, dried under reduced pressure for 24 hours, and weighed to calculate the weight of the solid extract from which the fat-soluble component was removed.
  • the composition and yield of the palm fruit fruit solid extract from the experimental values were calculated and shown in Table 1.
  • Polydicyandiamide (polydicyandiamide, DC-400, SNF Korea, Korea) was treated in a liquid extract at a concentration of 3,000 mg / L and stirred at a slow rate to cause the emulsion particles to coagulate in the fine biomass.
  • Cationic polyacrylamide (polyacrylamide C-211, SNF Korea, Korea) was added thereto at a concentration of 30 mg / L, and stirred for 60 minutes at room temperature to form an aggregate. After the aggregate was formed, the solid extract was prepared by solid-liquid separation using a small filter press (Korea Institute of Chemical Research, Korea) attached to the 6 micron filter cloth. The solid extract was weighed, taken in small amounts and lyophilized to determine the moisture content.
  • the solid extract was weighed, taken in small amounts and lyophilized to determine the moisture content.
  • 2 g of this solid extract was taken in a 50 ml falcon tube, followed by ultrasonic extraction with 9 ml of ethanol, followed by centrifugation to prepare a supernatant.
  • Carotenoids were quantified as indicators of fat-soluble bioactive substances by a spectrophotometer (Benchmark Plus microplate spectrophotometer, BIO RAD, USA, 450 nm). The ethanol remaining in the falcon tube was decanted and blown with air to remove the ethanol by volatilization.
  • the dried palm fruits and palm pulp fibers were cut and pulverized with a cutter mill (manufactured by Korea Powder Machinery, Korea) to prepare a powder of 20 mesh or less. Palm oil plant wastewater was lyophilized and ground to a powder of 20 mesh or less. 5 g (during drying) of these samples were taken and placed in a Soxhlet wobble, followed by Soxhlet extraction using ethanol as a solvent. The volume of the extract was measured and a portion was taken and centrifuged.
  • Beta carotene was quantified as an indicator of fat-soluble physiologically active substance, and the recovery rate of each example was calculated by comparing this value with the amount of carotenoid or beta carotene obtained in Examples, and the results are shown in Table 1.
  • Palm By-Products Composition (g) Example 1 (palm fruits and vegetables) Example 2 (palm pulp fiber) Example 3 (palm oil factory wastewater) Total amount 35.5 32.1 32.9 water 20.5 17.4 19.4 Insoluble Biomass 8.6 8.4 4.5 Fat-soluble ingredients 6.4 6.3 9.0 Beta-carotene or carotenoids (mg) in biomass (300g) 10.5 (carotenoids) 19.8 (beta carotene) 14.5beta carotene) Beta-carotene or carotenoids (mg) in solid extract 4.8 10.1 12.3 Carotene or carotenoid recovery rate (%) 45 51 85

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Physics & Mathematics (AREA)
  • Thermal Sciences (AREA)
  • Extraction Or Liquid Replacement (AREA)

Abstract

La présente invention concerne un extrait en phase solide contenant, sous une concentration élevée, une substance physiologiquement active soluble dans les matières grasses, telles que les caroténoïdes et le tocole, produites à partir des sous-produits générés durant un procédé de production d'huile de palme, et son procédé de production. Dans la mesure où une substance physiologiquement active soluble dans les matières grasses, qui est contenue dans la biomasse de palme sous une concentration faible et peut uniquement être extraite en utilisant une grande quantité de solvant, est extraite en utilisant seulement de l'eau et ensuite adsorbée sur une petite quantité de biomasse à solidifier, la substance physiologiquement active soluble dans les matières grasses peut être par la suite extraite et récupérée même après l'extraction avec une petite quantité de solvant, moyennant quoi la présente invention peut produire des matières premières utiles pour la production industrielle de diverses substances physiologiquement actives possédées par la biomasse de palme. De plus, la présente invention est un procédé utile pour améliorer la faisabilité économique du bioraffinage en augmentant la valeur ajoutée des sous-produits de production d'huile de palme.
PCT/KR2017/013717 2016-12-02 2017-11-28 Extrait de biomasse de palmier à huile en phase solide contenant une concentration élevée de substance physiologiquement active soluble dans les matières grasses produites à partir des sous-produits de production d'huile de palme et son procédé de production WO2018101710A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
KR10-2016-0163850 2016-12-02
KR1020160163850A KR101952929B1 (ko) 2016-12-02 2016-12-02 팜유 제조 부산물로부터 제조한 지용성 생리활성물질을 고농도로 포함하는 오일팜 바이오매스 고상추출물 및 그의 제조 방법

Publications (1)

Publication Number Publication Date
WO2018101710A1 true WO2018101710A1 (fr) 2018-06-07

Family

ID=62242669

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/KR2017/013717 WO2018101710A1 (fr) 2016-12-02 2017-11-28 Extrait de biomasse de palmier à huile en phase solide contenant une concentration élevée de substance physiologiquement active soluble dans les matières grasses produites à partir des sous-produits de production d'huile de palme et son procédé de production

Country Status (2)

Country Link
KR (1) KR101952929B1 (fr)
WO (1) WO2018101710A1 (fr)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2020141967A1 (fr) * 2019-01-03 2020-07-09 Palmite Process Engineering Sdn Bhd Procédé de conversion d'un effluent liquide de moulin à huile de palme en une biomasse solide pour faciliter le recyclage

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5190618A (en) * 1988-03-16 1993-03-02 Bioindustry Development Centre (Bidec) Production of high concentration tocopherols and tocotrienols from palm-oil by-products
KR101029879B1 (ko) * 2011-02-09 2011-04-15 주식회사 덕분 팜 부산물을 이용한 친환경 저황 함유 바이오 벙커 ｃ유의 제조 방법
US8048652B2 (en) * 2005-05-12 2011-11-01 Martek Biosciences Corporation Biomass hydrolysate and uses and production thereof
KR20130115577A (ko) * 2012-04-12 2013-10-22 주식회사 창해에탄올 고농도 발효성 당의 제조 장치, 이를 이용한 고농도 발효성 당의 제조 방법, 및 이를 이용하여 제조된 바이오 연료 및 바이오 화학물질

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
MY162051A (en) 2007-05-24 2017-05-31 Loders Croklaan Bv Process for producing compositions comprising tocopherols and tocotrienols

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5190618A (en) * 1988-03-16 1993-03-02 Bioindustry Development Centre (Bidec) Production of high concentration tocopherols and tocotrienols from palm-oil by-products
US8048652B2 (en) * 2005-05-12 2011-11-01 Martek Biosciences Corporation Biomass hydrolysate and uses and production thereof
KR101029879B1 (ko) * 2011-02-09 2011-04-15 주식회사 덕분 팜 부산물을 이용한 친환경 저황 함유 바이오 벙커 ｃ유의 제조 방법
KR20130115577A (ko) * 2012-04-12 2013-10-22 주식회사 창해에탄올 고농도 발효성 당의 제조 장치, 이를 이용한 고농도 발효성 당의 제조 방법, 및 이를 이용하여 제조된 바이오 연료 및 바이오 화학물질

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
KURNIN, NOR AZRIN AHMAD: "Recovery of palm oil and valuable material from oil palm empty fruit bunch by sub-critical water", JOURNAL OF OLEO SCIENCE, vol. 65, no. 4, April 2016 (2016-04-01), pages 283 - 289, XP055606983 *

Also Published As

Publication number Publication date
KR101952929B1 (ko) 2019-02-27
KR20180063730A (ko) 2018-06-12

Similar Documents

Publication Publication Date Title
Diamanti et al. Green extraction of polyphenols from whole pomegranate fruit using cyclodextrins
CN110304994B (zh) 一种从工业大麻中提取高纯度大麻二酚的方法
Ahmad et al. Recovery of oil and carotenes from palm oil mill effluent (POME)
Takenaka et al. Effective extraction of curcuminoids by grinding turmeric (Curcuma longa) with medium-chain triacylglycerols
CN106794394A (zh) 用于破乳以及将乳液中有机化合物复合的方法和装置
CN108558971A (zh) 一种玫瑰茄花色苷的制备方法
Kuan et al. Physical, chemical and physicochemical characterization of rice husk
US20180338513A1 (en) METHOD FOR REMOVING BENZO[a]PYRENE FROM LIPOSOLUBLE NATURAL EXTRACT
WO2018101710A1 (fr) Extrait de biomasse de palmier à huile en phase solide contenant une concentration élevée de substance physiologiquement active soluble dans les matières grasses produites à partir des sous-produits de production d'huile de palme et son procédé de production
Sinichi et al. Recovery of phenolic compounds from the by-products of yellow mustard protein isolation
CN104256640B (zh) 一种从人心果叶子提取天然抗氧化物质的方法
DK2904075T3 (en) ETHANOL HEAT EXTRACTION OF PLANT OR ANIMAL MATERIAL LIPIDS
US8007838B2 (en) Process for producing a stable concentrated dietary supplement and supplement produced thereby
CN1314789C (zh) 纯鸦胆子油的制备方法
CA3114540A1 (fr) Methodes d`extraction de cannabinoides du cannabis, y compris le cannabis frais
CA2795413C (fr) Procede d'extraction d'huile de graines comprenant une etape de broyage de graines dans un solvant
Sangkharak et al. Separation of carotenes and vitamin e from palm oil mill effluent using silica from agricultural waste as an adsorbent
CN1208332C (zh) 天然生育酚提取工艺
CN1528758A (zh) 大豆异黄酮主要单体组分的分离方法
JP4641759B2 (ja) シリコーン油の精製方法
CN105944403A (zh) 一种再造烟叶原料提取液的净化方法
Lozano-Sánchez et al. Extraction, Isolation and Utilisation of Bioactive Compounds from Waste Generated by the Olive Oil Industry
CN114099570A (zh) 一种低损耗的火麻仁小分子提取物的制备方法
CN116904198A (zh) 化学机械浆预浸渍段废弃溶出物在制备抗氧化剂上的应用
CN107868695A (zh) 一种脱毒麻疯树油的制备方法

Legal Events

Date Code Title Description
121 Ep: the epo has been informed by wipo that ep was designated in this application

Ref document number: 17877018

Country of ref document: EP

Kind code of ref document: A1

NENP Non-entry into the national phase

Ref country code: DE

122 Ep: pct application non-entry in european phase

Ref document number: 17877018

Country of ref document: EP

Kind code of ref document: A1