WO2018080281A1 - Apparatus and method for modifying skin by using laser and plasma - Google Patents

Abstract

The present invention relates to a method for modifying skin by using a laser and a plasma and an apparatus therefor, the apparatus comprising: a laser generator configured to radiate a laser with a power applied from a power supply device; and a plasma generator configured to receive a power from the power supply device, to generate a potential difference, and thus to generate a plasma. In the apparatus, the laser stimulates the skin and the scalp while the plasma penetrates the stimulated skin and scalp, thereby increasing a skin regeneration effect.

Description

Skin Modification Device and Modification Method Using Laser and Plasma

The present invention relates to a skin reforming apparatus and a method for reforming using a laser and plasma, and more particularly, to a skin reforming apparatus and a method for reforming using a laser and plasma to stimulate the skin and scalp to promote regeneration.

Human skin tissue is composed of the epidermis and the subcutaneous tissue below it, the epidermal layer having a stratum corneum acts as a biological barrier to the environment, the melanin cells that determine the skin color is present in the base layer. In addition, the lower subcutaneous tissue maintains the structure of the skin by the extracellular collagen acts as a support. Collagen is synthesized by fibroblasts and is a protein in which three polypeptides form a spiral structure.

When injured, the body activates a complex remedy, of which fibroblasts are present under the skin and are activated to temporarily enter the extra cellular matrix (ECM) to cover the wound as soon as possible. Secrete. As fibroblasts slowly move around the matrix, attracting fibers to recombine the matrix, making the matrix more rigid, and because of the growth factors contained in the matrix that were initially soft, the fibroblasts expressed contractile proteins by the growth factors, At some point, the fibroblasts stop moving and turn into strong contractile cells, such as popeye, that settle in the matrix and attract the edges of the wound, resulting in scarring or uneven surface of the skin.

At this time, the collagen fibrous tissue is heated by a predetermined energy source, degeneration of the physical properties of the protein matrix occurs at a specific temperature, molecular binding of the matrix is broken, and the condition of the skin becomes even, and also activates the collagen layer. The elasticity of the skin can be restored.

On the other hand, the solid with the lowest energy state among the materials receives energy and is transferred to a liquid or gas. In this case, when energy more than ionization energy is applied to the neutral gas, a plasma including electrons, ions, neutral atoms, and molecules may be generated by ionization and recombination of electrons and ions. Plasma is called ionized conductive gas species because the positive and negative charges are mixed in the plasma to maintain Brown's motion close to free particles and to remain electrically neutral. Plasma classification criteria include plasma density, electron temperature, degree of thermal equilibrium (LTE or non-LTE) between species, generation mode, and application field. Plasma for research and manufacturing processes is usually either LTE or non-LTE, and the former is commonly referred to as thermal plasma and the latter as low temperature plasma. Atmospheric pressure plasma in low temperature plasma is mainly used in the field of surface modification and coating, environmental purification. In recent years, research has been expanded to include bioapplications and biomedical applications. In the field of biomedical, many atmospheric pressure plasma apparatuses have been studied (Korean Patent No. 1407672 and Korean Patent No. 1292268).

However, the use of a technique of recovering skin elasticity by using a source of energy such as a laser to restore skin elasticity and a technique of modifying the skin using plasma, respectively, are limited in their effects. Thus, there is an increasing need for the development of techniques for using these together to further enhance the skin and scalp's modifying effects.

Applicants of the present invention thus completed the present invention. The present invention relates to a device for modifying skin and scalp using a laser and a plasma, and to a method for modifying the plasma and the plasma according to the present invention, since hair growth from the scalp promotes hair growth and significantly increases the proliferation of dermal papilla cells. It is expected to be used greatly.

[Preceding technical literature]

(Patent Document 0001) Republic of Korea Patent Publication No. 10-1407672 (2006.07.04.)

The present invention was created to improve the problems of the skin and scalp reforming method using a conventional laser and plasma as described above, the skin and scalp reforming apparatus using a laser and plasma to induce regeneration of the skin by laser and plasma And to provide a method.

In order to achieve the object as described above, the hair growth promoting apparatus according to the present invention includes a laser generator configured to irradiate a laser beam by a power applied from a power supply device, and a power supply from the power supply device to generate a potential difference, thereby generating a plasma. Characterized in that it comprises a plasma generating portion formed to generate. In addition, the skin modification method using the device is characterized in that it comprises a laser irradiation step of irradiating the laser to the skin, and a plasma treatment step of treating the plasma to the skin.

In this case, the control unit may further include a controller connected to the power supply device and configured to control power applied to the laser generator and the plasma generator.

On the other hand, the laser generating unit and the plasma generating unit may be provided independently of each other to operate individually.

In addition, the laser generating unit and the plasma generating unit may further include a handpiece body coupled.

In this case, the laser generation unit may be connected to one end of the handpiece body, and may generate a laser having a wavelength of 500 nm or more and 700 nm or less using LD or LED as a light source.

In addition, the plasma generator may be disposed to be spaced apart from the laser generator, and may generate a plasma by a potential difference, and a pinhole may be formed to pass the laser generated by the laser generator.

On the other hand, it is also possible to further include a bridge extending from the end portion of the laser generating portion side of the handpiece body to be connected to the plasma generating portion to prevent damage to the skin.

On the other hand, the plasma generating unit may include a plasma electrode for generating a dielectric barrier plasma, and an electrode holder formed to receive the plasma electrode.

In addition, the plasma generating unit may further include a spacer which protrudes to contact the skin to generate a predetermined space between the skin and the plasma electrode.

In this case, the plasma electrode may include a metal electrode generating a potential difference using an applied voltage, and a dielectric layer formed to stabilize the plasma by surrounding the metal electrode.

In addition, the plasma electrode may have the metal electrode and the dielectric layer formed in a lattice form so that the pinhole is formed.

The bridge may be extended to correspond to the laser focusing distance of the laser generation unit.

Meanwhile, the controller may alternately operate the laser generator and the plasma generator.

The controller may operate the laser generator and the plasma generator at the same time.

On the other hand, the power supply device may output a current in the form of a sine wave (sine wave).

In the skin and scalp reforming method using the apparatus, it is also possible to generate a low-temperature plasma to the skin in the plasma treatment step and to treat the skin.

It is also possible to include pulse streamer discharges, silent discharges, partial discharges, creepage discharges and corona discharges.

Meanwhile, in the laser irradiation step, it is also possible to irradiate the skin with a peelable laser.

In addition, in the laser irradiation step, it is also possible to irradiate the laser to the skin by a low power laser therapy method.

On the other hand, it is also possible to overlap the skin region for irradiating a laser in the laser irradiation step and the skin region for generating a plasma in the plasma processing step.

In this case, it is also possible to perform the laser irradiation step and the plasma processing step at the same time.

The skin modification method may also be performed after the laser irradiation step.

In addition, the skin modification method may be to perform the laser irradiation step after the plasma treatment step.

It is also possible to alternately perform the laser irradiation step and the plasma generation step.

In addition, the laser irradiation step and the plasma generating step may be performed according to a predetermined pattern.

In this case, the laser may be irradiated with a wavelength of 1500 nm or more and 11000 nm or less.

The laser may be irradiated with a wavelength of 500 nm or more and 700 nm or less.

On the other hand, the device of the present invention is a cosmetic use for skin modification, and may further be used to improve skin wrinkles or to promote transdermal absorption of compositions such as cosmetics. "Beauty" in the present invention is intended to beautify the appearance of a healthy subject, and is clearly distinguished from the concept of "treatment" performed in a subject having a disease.

In one embodiment of the invention, the laser generating unit is formed so that the laser is irradiated by the power applied from the power supply; And a plasma generator configured to receive power from the power supply, and generate a potential difference to generate plasma, wherein the hair growth promoting device is connected to the power supply and is connected to the laser generator and the plasma. And a control unit provided to control the power applied to the generating unit. The hair growth promoting apparatus further comprises: the laser generating unit and the plasma generating unit are provided independently of each other to operate individually. It provides a hair growth promoting device, and provides a hair growth promoting device further comprises; a handpiece body coupled to the laser generator and the plasma generator.

In the apparatus, the laser generating unit is connected to one end of the handpiece body, the LD or LED as a light source, the hair growth promoting device, characterized in that for generating a laser having a wavelength of 500nm or more, 700nm or less to provide.

In the above apparatus, the plasma generating unit is arranged to be spaced apart from the laser generating unit, generating a plasma by the potential difference, the hair growth promoting device characterized in that the pinhole is formed to pass the laser generated in the laser generating unit And a bridge extending from the laser generating portion side end of the handpiece body to be connected to the plasma generating portion to prevent damage to the skin.

In the above apparatus, the plasma generating unit includes: a plasma electrode for generating a dielectric barrier plasma; And an electrode holder formed to receive the plasma electrode, wherein the plasma generating unit is formed to protrude to generate a predetermined space between the skin and the plasma electrode in contact with the skin. It provides a hair growth promoting apparatus further comprising a spacer.

In the device, the plasma electrode, a metal electrode for generating a potential difference using an applied voltage; And a dielectric layer formed to surround the metal electrode to stabilize the plasma, wherein the plasma electrode comprises: the metal electrode and the dielectric layer formed in a lattice form such that the pinhole is formed. It provides a hair growth promoting device, characterized in that.

In the above apparatus, the bridge is provided in accordance with the laser focusing distance of the laser generating unit, characterized in that the hair growth promoting device, characterized in that the control unit alternately to operate the laser generating unit and the plasma generating unit. A hair growth promoting apparatus is provided, wherein the controller provides the hair growth promoting apparatus, wherein the laser generating unit and the plasma generating unit are operated at the same time, and the power supply device is in the form of a sine wave (sine wave). Provided is a hair growth promoting device, characterized by outputting a current.

In another embodiment of the present invention, there is provided a skin modification method comprising the step of co-processing the plasma and light on the skin, wherein the co-treatment of the plasma and light, the plasma pre-treatment and post-treatment, or the light It provides a skin modification method of pretreatment and post-treatment of plasma, simultaneous treatment of plasma and light, or alternately repeating treatment of plasma and laser, wherein the skin modification is performed on the skin of the plasma-treated area and of the light-treated area. Provided is a method of skin modification characterized in that the skin is superimposed.

In the skin modification method, the plasma is a skin modification method is a low-temperature plasma, the low temperature plasma is a skin modification method is a plasma by pulse streamer discharge, silent discharge, partial discharge, creeping discharge, or corona discharge. , Wherein the light is a skin modification method, wherein the laser is a skin modification method, which is a skin-derived laser, the laser is a skin modification method, which is a laser of low power laser therapy, and the light is a skin characterized in that the wavelength of 605nm to 650nm. Provide a method of reforming.

In addition, in the skin modification method, the skin is a skin modification method that is the scalp, and the skin modification provides a skin modification method of inhibiting hair loss, promoting hair growth, or strengthening the hair root.

In addition, in the skin modification method, the plasma is a skin modification method for treating 5 to 40 J / cm ² to the skin, the plasma is a skin modification method to treat 10 to 20 J / cm ² to the skin, the light Is a skin modification method for treating the skin at 2 to 16 J / cm ² , and the light provides a skin modification method for treating the skin at 2 to 8 J / cm ² .

Hereinafter, the present invention will be described in detail step by step.

As described above, according to the skin and scalp modifying apparatus and method using the laser and the plasma according to the present invention, the laser and the scalp are stimulated, and the plasma is penetrated into the irritated skin and the scalp to increase the regeneration effect. It is effective in promoting hair growth.

1 is a flowchart illustrating a method for reforming skin and scalp using laser and plasma according to an embodiment of the present invention;

FIG. 2 is a block diagram of an apparatus for performing skin and scalp modification using laser and plasma according to an embodiment of the present invention; FIG.

3 is a perspective view of a device for performing the method of skin and scalp modification using a laser and plasma according to an embodiment of the present invention,

4 is an enlarged perspective view of a plasma generator and a laser generator in FIG. 3;

5 is a front view of the plasma generator in FIG. 3;

FIG. 6 is a cross-sectional view showing a cross section of a portion indicated by A-A in FIG. 5;

7 is a result showing the scalp stimulation effect using a laser and a plasma according to an embodiment of the present invention.

In order to measure the plasma energy range suitable for stimulating hair growth of the scalp when co-administering plasma and light, plasma of various energies was combined with red wavelength light in human hair follicle dermal papilla cells.

As a result of the experiment, when the plasma is treated alone, the energy range of the plasma is preferably 5 to 40 J / cm ² , more preferably 10 to 20 J / cm ² . In the case where the plasma and the light of the red wavelength are used in combination, the energy range of the plasma is preferably 5 to 20 J / cm ² and the light of the red wavelength is 2 to 16 J / cm ² , and the energy of the plasma is 10 to More preferably, the light of 20 J / cm ² and the red wavelength is 2 to 16 J / cm ² , the energy range of the plasma is 10 to 20 J / cm ² and the light of the red wavelength is 2 to 8 J / cm ² . Has been shown to be more desirable.

Hereinafter, the present invention will be described in more detail with reference to Examples. These examples are only for illustrating the present invention in more detail, and it will be apparent to those skilled in the art that the scope of the present invention is not limited by these examples according to the gist of the present invention. .

Example 1 Fabrication of Plasma and Light (Laser) Expression Apparatus

With reference to the drawings of the present invention will be described in detail a preferred embodiment of the present invention. In the description and drawings of the present invention, reference numeral 100 denotes a handpiece body, 200 denotes a laser generator, 300 denotes a plasma generator, 310 denotes an electrode holder, 330 denotes a plasma electrode, and 331 denotes a metal electrode, 333 denotes a dielectric layer, 335 denotes a pinhole, 350 denotes a spacer, 400 denotes a bridge, 500 denotes a power supply, and 600 denotes a control unit. .

Referring to FIG. 1, a method for modifying skin and scalp using a laser and a plasma according to an embodiment of the present invention includes a laser irradiation step (S10) and a plasma processing step (S20).

In this case, the laser irradiation step (S10) and the plasma processing step (S20) is not only performed independently of each other, but also after the laser irradiation step (S10) to perform the plasma processing step (S20) or the plasma processing step (S20) After the laser irradiation step (S10) may be performed, the laser irradiation step (S10) and the plasma processing step (S20) may be performed simultaneously or alternately with each other, and performed according to a predetermined pattern. It is also possible.

In the laser irradiation step (S10) is irradiated with a laser on the skin (scalp). At this time, the laser irradiated to the skin (scalp) includes a peelable laser. In the case of a peelable laser, lasers of various wavelengths (1500 nm or more and 11000 nm or less), including a CO ₂ laser (wavelength 10600 nm), an erbium yag laser (Erbium YAG: wavelength 2940 nm), and a fractional laser (wavelength 1550 nm), are applicable. . Dermabrasive lasers have the effect of irritating the skin's epidermis (CO ₂ laser and Erbium Yag laser) or dermis (fractional laser) to regenerate the skin.

On the other hand, in the laser irradiation step (S10) it is also possible to use a laser used for Low Level Laser Treatment (LLLT). That is, by irradiating a laser having a wavelength of 500nm or more and 700nm or less, there is an effect of expanding the blood vessels and improving blood circulation to restore the skin.

In addition, in the laser irradiation step S10, a laser (energy source) having a wavelength of 500 nm or more and 700 nm or less and using a laser diode (LD) or a light emitting diode (LED) as a light source may be used. Hair repeats three stages of growth, rest, and degeneration, called hair cycles. The laser having the above wavelength (wavelength of 500 nm or more and 700 nm or less) stimulates metabolism by stimulating hair follicles of the resting and degeneration phases. Since the above wavelength (wavelength above 500nm and below 700nm) is specifically absorbed by blood vessels and hair follicles compared to other wavelengths, the above effect can be expected. (Reference paper: Eells JT Wong-Riley MT VerHoeve J., et al.Mitochondrial signal transduction in accelerated wound and retinal healing by near-infrared light therapy.Mitochondrion)

In the plasma treatment step (S20), the plasma is treated on the skin (scalp). At this time, the plasma irradiated to the skin (scalp) includes a low temperature plasma. Discharges forming low temperature plasma include pulse streamer discharge, silent discharge, partial discharge, creepage discharge and corona discharge. The pulse streamer discharge is applied to a pulsed high voltage with a rise time of several tens of milliseconds and a duration of about 1 milliseconds or less, and a streamer discharge occurs to form a plasma. The application of alternating current high voltage causes pulsed discharge, and the spray discharge is the installation of metal mesh electrodes on both sides of the filling of the ceramic ferret with a particle diameter of several mm and the application of alternating current high voltage. It happens in the middle.

In particular, in the case of the dielectric barrier plasma belonging to the silent discharge, it is possible to generate a controlled plasma while passing through the dielectric layer, thereby preventing damage to a target material including a living tissue.

Meanwhile, in the present invention, the skin (scalp) region irradiated with the laser in the laser irradiation step S10 and the skin (scalp) region where the plasma is treated in the plasma processing step S20 are overlapped. In this case, unlike the conventional effect of stimulating the skin with a laser to induce a regeneration effect, or simply irritating the epidermis of the skin by plasma, the laser irradiation step (S10) to stimulate the skin (scalp) and hair follicles and the It is possible to maximize the regeneration and irritation effect of the skin by infiltrating the plasma to the dermal layer in the plasma treatment step (S20), or to stimulate the skin (scalp) and hair follicles with plasma in the plasma treatment step (S20) and the laser irradiation step In (S10) it is possible to maximize the recovery and regrowth effect of the skin by improving blood circulation by expanding and stimulating blood vessels through the low power laser.

2 shows a hair growth promoting device according to an embodiment of the present invention. The hair growth promoting apparatus using the laser and the plasma according to the present embodiment includes a laser generator 200, a plasma generator 300, a power supply device 500, and a controller 600. In this case, power is applied from the power supply device 500 to the controller 600, and the power applied to the controller 600 is controlled by the controller 600 and the plasma generator 200 and the plasma. It is electrically connected to the generator 300 to be applied.

At this time, the laser generating unit 200 is a peeling of various wavelengths (1500nm or more, 11000nm or less), including a CO ₂ laser (wavelength 10600nm), Erbium Yag laser (Erbium YAG: wavelength 2940nm) and fractional laser (wavelength 1550nm). Sex lasers can be applied.

On the other hand, the laser generator 200 may be provided to generate a low power laser having a wavelength of 500nm or more, 700nm or less.

In addition, the laser generator 200 may be provided with a laser diode (LD) or a light emitting diode (LED) having a wavelength of 500 nm or more and 700 nm or less.

2 to 6 show specific embodiments of the skin modification apparatus using the laser and the plasma of the present invention. The skin reforming apparatus using the laser and the plasma of the present embodiment includes the handpiece body 100, the laser generator 200, the plasma generator 300, the bridge 400, the power supply device 500, and the controller 600. Include. In this case, the plasma generator 300 is coupled to one end of the handpiece body 100, and the bridge 400 is formed to extend from the plasma generator 300 side end of the handpiece body 100. The bridge 400 is coupled to the plasma generator 300.

The handpiece body 100 forms an outer shape of the present invention, has a cylindrical shape formed along the axial direction, and accommodates the power supply device 500 and the controller 600 therein.

The laser generator 200 is coupled to one end of the handpiece body 100 and includes a microarray lens (not shown).

5 and 6, the plasma generator 300 includes an electrode holder 310, a plasma electrode 330, and a spacer 350. In this case, the plasma electrode 330 is seated inside the electrode holder 310, and the spacer 350 protrudes outward from the electrode holder 310.

Meanwhile, in the present embodiment, the plasma generating unit 300 applies a dielectric barrier plasma, but is not limited thereto. The plasma generator 300 includes a low temperature plasma using a streamer discharge, a dielectric barrier discharge, a creepage discharge, a corona discharge, or the like.

The electrode holder 310 is connected to one end of the bridge 400, is formed in a disk shape as a whole, it is formed in the shape of a hole so that the plasma electrode 330 can be seated inside.

The plasma electrode 330 includes a metal electrode 331, a dielectric layer 333, and a pinhole 335. On the other hand, the plasma electrode 330 is coupled to the electrode holder 310 to be detachable.

In this case, the metal electrode 331 is electrically connected to the power supply device 500 and the controller 600, and the surface of the metal electrode 331 is bonded to the dielectric layer 333 and covered. In addition, the metal electrode 331 is formed in a lattice form.

The dielectric layer 333 is stacked on the metal electrode 331 with a uniform thickness. Accordingly, the metal electrode 331 is formed in the form of a lattice, and the pinhole 335 is formed between the dielectric layers 333 in the form of a lattice.

Meanwhile, in the present embodiment, the dielectric layer 333 uses a ceramic material. However, the dielectric layer 333 is not limited thereto, and alumina (Al ₂ O ₃ ), zirconia (ZrO ₂ ), or the like may be used as the dielectric material.

The spacer 350 protrudes outward from the electrode holder 310 and is formed along the circumferential direction, and a plurality of spacers 350 are formed at predetermined intervals. In addition, the spacer 350 is formed to be in contact with the user's skin. In the present embodiment, four spacers 350 are formed at intervals of 90 degrees, but are not limited thereto.

The bridge 400 extends from an end portion of the laser generating unit side of the handpiece body 100 to be connected to the plasma generating unit 300. In the present embodiment, two bridges 400 are formed, but are not limited thereto.

The bridge 400 is formed corresponding to the focal length of the laser generator 200.

Referring to FIGS. 4 and 5, the operation and effect of the apparatus for implementing the skin and scalp modification method using the laser and the plasma according to the present embodiment will be described. A user grabs the handpiece body 100 and contacts the skin. Let's do it. At this time, the spacer 350 is in contact with the skin to form a space between the plasma generating unit 300 and the skin.

When the user operates the hair growth promoting device using the laser and the plasma of the present invention, the laser generator 200 may skin a laser having a wavelength of 500 nm or more and 700 nm or less in a laser diode (LD) or a light emitting diode (LED). Investigate (scalp).

In addition, when the skin reforming apparatus using the laser and the plasma of the present invention is operated, power is applied to the metal electrode 331 of the plasma generating unit 300 through the power supply device 500 and the control unit 600. do. On the other hand, in the present embodiment, the dielectric barrier plasma is generated, the power source uses a low frequency of 30 or more and 99 kHz or less for the dielectric barrier plasma processing, the power consumption of the plasma processing is 1 or more and 15 W or less, and the ground current of the plasma processing. Is not less than 0.1 and less than 9.9 mA, but is not limited thereto.

In this case, the power supply device 500 may supply an output current of a sin (sine) waveform (sine wave). When the output current waveform is obtained as a sin waveform, the efficiency compared to the input voltage can be further increased compared to a general square wave (square wave). In addition, in the case of a square wave, a surge phenomenon occurs, and when a plasma is generated, a large current flows rapidly for a short time, but the sin wave output has the advantage of enabling stable plasma. When power is applied to the metal electrode 331, plasma is generated by the potential difference between the plasma electrode 330 and the skin.

At this time, the energy generated by the potential difference in the metal electrode 331 is stabilized while passing through the dielectric layer 333 to prevent high energy from being concentrated at the localized portion. Therefore, the plasma is generated while passing through the dielectric layer 333 (dielectric barrier plasma) to prevent damage to the target material, including biological tissues.

In the present embodiment, since the dielectric layer 333 is not formed only on one side thereof but is formed to surround the metal electrode 331, the plasma generation unit as well as the skin and the plasma generation unit 300 are separated from each other. Plasma is generated even when the skin side of 300 is in contact with the skin.

In the present exemplary embodiment, the metal electrode 331 and the dielectric layer 333 are formed in a lattice form to form the pinhole 335, and the laser generating unit 200 and the plasma generating unit are formed by the bridge 400. 300 was placed coaxially. That is, the skin region for irradiating a laser from the laser generator 200 and the skin region for generating plasma from the plasma generator 300 overlap each other.

Therefore, the laser irradiated from the laser generator 200 may pass through the pinhole 335 to irradiate the skin. In addition, the length of the bridge 400 may be formed to correspond to a laser focusing distance, thereby providing an optimal condition to prevent plasma damage while penetrating the plasma.

This causes the skin regeneration effect by using a laser or, unlike the effect of simply stimulating the epidermis of the skin by plasma, irritation is applied to the skin by the laser generated in the laser generator 200, In this state, the plasma generated by the plasma generator 300 penetrates to the dermis layer to maximize the regeneration and stimulation effect of the skin.

On the other hand, in the present invention, the plasma electrode 330 is detachably coupled, and designed to adjust the intensity of the plasma and the irradiation amount of the laser according to the type of skin.

In addition, in the present invention, the control unit 600 not only operates the laser generator 200 and the plasma generator 300 at the same time, but also operates the laser generator 200 and then the plasma generator 300. ) May be operated, and conversely, the plasma generator 300 may be operated first. In addition, the laser generating unit 200 and the plasma generating unit 300 may be alternately operated. Alternatively, the laser generating unit 200 and the plasma generating unit 300 may be operated in accordance with a predetermined pattern.

Example 2. Confirmation of VEFG Protein Expression According to Light Wavelength

Using the plasma and laser generating apparatus described in Example 1 of the present invention, the expression level of the vascular endothelial growth factor (VEGF) according to the wavelength of light simultaneously treated with the plasma was investigated. VEGF is a glycoprotein that specifically acts on vascular endothelial cells to promote cell proliferation and angiogenesis. It is known to stimulate hair growth of the scalp to promote hair growth. To this end, the hairs of rats (SD rat, male, 8 weeks old) were regrown, sterilized with 70% ethanol, and randomly selected 5 mice per group, and treated with plasma as shown in Table 1 below.

group	Remarks
Control	Untreated
Plasma	Plasma 15J / cm 2 Treatment
Blue	5 J / cm 2 light with 440nm ~ 490nm (465nm peak) wavelength
Blue + Plasma	Plasma 15J / cm 2 and light 5 J / cm 2 with 440nm to 490nm (465nm peak) wavelength
Green	5 J / cm 2 light of 485nm ~ 555nm (520nm peak) wavelength
Green + Plasma	Plasma 15J / cm 2 and 5 J / cm 2 light at 485nm to 555nm (520nm peak) wavelength
Yellow	5 J / cm 2 light at 570 nm to 610 nm (590 nm peak) wavelength
Yellow + plasma	Plasma 15J / cm 2 and light 5 J / cm 2 with 570nm to 610nm (590nm peak) wavelength
Red	5J / cm 2 light with 605nm ~ 650nm (625nm peak) wavelength
Red + Plasma	Plasma 15J / cm 2 and light 5 J / cm 2 with 605nm to 650nm (625nm peak) wavelength

After treatment as in Table 1, after 30 minutes, the rat scalp was obtained, and the protein expression level of VEGF was measured by Western blotting. The results are shown in FIG.

As a result of the experiment, only the plasma treatment showed a doubling of VEGF expression compared to the case of no treatment. However, the treatment of light only showed a slight difference in VEGF expression regardless of the wavelength of light. . Even when the plasma and the light are treated simultaneously, the wavelength of the light is blue (440 nm to 490 nm), green (485 nm to 555 nm), or yellow (570 nm to 610 nm). Although there was no difference, when the plasma and the red wavelength (605nm ~ 650nm) is treated together, it can be seen that the expression of VEGF significantly increased compared to all other cases.

Example 3. Confirmation of changes in dermal papilla cells according to the plasma energy amount

In order to measure the plasma energy range suitable for stimulating hair growth of the scalp when co-administering plasma and light, plasma of various energies was combined with red wavelength light in human hair follicle dermal papilla cells.

First, incubate the dermal papilla cells (CB-HDP-001, Serin Bioscience, Korea) at a density of 5 × 10 ⁵ / well in a 24-well plate, remove the cultures, wash twice with PBS, and then per well Plasma 0 to 40 J / cm ² , and red light (605 nm to 650 nm (625 nm peak)) of light 0 to 16 J / cm ² were treated with 50 μl of PBS added.

Thereafter, the degree of proliferation of dermal papilla cells was measured by MTT assay.

Table 2 shows the results of% conversion of the MTT measurement results in each sample on the basis of the negative control group (plasma and light untreated), and the results of% conversion on the basis of untreated light in the same plasma energy group are shown in Table 3 below. Described.

% of control		Plasma Energy (J / cm 2 )
		0	5	10	20	40
Red wavelength light (J / cm 2 )	0	100	112	132	145	111
	2	115	121	183	176	105
	4	118	127	216	198	109
	8	127	131	202	223	101
	16	122	128	132	141	97

% of plasma		Plasma Energy (J / cm 2 )
		0	5	10	20	40
Red wavelength light (J / cm 2 )	0	100	100	100	100	100
	2	115	108	139	121	95
	4	118	113	164	137	97
	8	127	117	153	154	91
	16	122	114	100	97	87

As a result of the experiment, when the plasma is treated alone, the energy range of the plasma is preferably 5 to 40 J / cm ² , more preferably 10 to 20 J / cm ² . In the case where the plasma and the light of the red wavelength are used in combination, the energy range of the plasma is preferably 5 to 20 J / cm ² and the light of the red wavelength is 2 to 16 J / cm ² , and the energy of the plasma is 10 to More preferably, the light of 20 J / cm ² and the red wavelength is 2 to 16 J / cm ² , the energy range of the plasma is 10 to 20 J / cm ² and the light of the red wavelength is 2 to 8 J / cm ² . Has been shown to be more desirable.

The rights of the present invention are not limited to the embodiments described above, but are defined by the claims, and various modifications may be made by those skilled in the art without departing from the technical scope of the present invention. It is obvious that it can be carried out by remodeling.

The present invention relates to a device for modifying skin and scalp using a laser and a plasma, and to a method for modifying the plasma and the plasma according to the present invention, since hair growth from the scalp promotes hair growth and significantly increases the proliferation of dermal papilla cells. It is expected to be used greatly.

Claims (30)

1. A laser generator configured to irradiate a laser by power applied from a power supply device; And

   A plasma generator configured to receive power from the power supply and generate a plasma by generating a potential difference;

   Hair growth promoting device comprising a.
2. The method of claim 1,

   A controller connected to the power supply device and configured to control power applied to the laser generator and the plasma generator;

   Hair growth promoting device further comprising a.
3. The method of claim 2,

   And the laser generating unit and the plasma generating unit are provided independently of each other to operate individually.
4. The method of claim 1,

   A handpiece body coupled to the laser generator and the plasma generator;

   Hair growth promoting device further comprising a.
5. The method of claim 4, wherein

   The laser generation unit,

   A hair growth promoting device connected to one end of the handpiece body and generating a laser having a wavelength of 500 nm or more and 700 nm or less, using LD or LED as a light source.
6. The method of claim 4, wherein

   The plasma generation unit,

   And a pinhole formed to be spaced apart from the laser generator, to generate a plasma by a potential difference, and to pass a laser generated by the laser generator.
7. The method of claim 6,

   A bridge extending from the end portion of the laser generation portion of the handpiece body to be connected to the plasma generation portion to prevent damage to the skin;

   Hair growth promoting device further comprising a.
8. The method of claim 1,

   The plasma generation unit,

   A plasma electrode generating a dielectric barrier plasma; And

   An electrode holder formed to receive the plasma electrode;

   Hair growth promoting device comprising a.
9. The method of claim 8,

   The plasma generation unit,

   A spacer protruding to contact the skin to generate a predetermined space between the skin and the plasma electrode;

   Hair growth promoting device further comprising a.
10. The method of claim 8,

    The plasma electrode,

    A metal electrode generating a potential difference using an applied voltage; And

    A dielectric layer formed around the metal electrode to stabilize the plasma;

    Hair growth promoting device comprising a.
11. The method of claim 10,

    The plasma electrode,

    And the metal electrode and the dielectric layer are formed in a lattice form so that the pinhole is formed.
12. The method of claim 7, wherein

    The bridge,

    And a hair growth promoting device extending in correspondence with the laser focusing distance of the laser generating unit.
13. The method of claim 2,

    The control unit,

    And the laser generating unit and the plasma generating unit are operated alternately.
14. The method of claim 2,

    The control unit,

    The hair growth promoting device, characterized in that for operating the laser generator and the plasma generator at the same time.
15. The method of claim 1,

    The power supply device,

    A hair growth promoting device for outputting a current in the form of a sine wave (sine wave).
16. A skin modification method comprising the step of combining plasma and light to the skin.
17. The method of claim 16,

    Combined treatment of the plasma and light,

    Plasma pretreatment and post-treatment of light, pretreatment of light and post-treatment of plasma, simultaneous treatment of plasma and light, or alternating treatment of plasma and laser alternately.
18. The method of claim 16,

    The skin modification is characterized in that for overlapping the skin of the plasma-treated area and the skin of the light-treated area, skin modification method.
19. The method of claim 16,

    Wherein said plasma is a low temperature plasma.
20. The method of claim 19,

    The low temperature plasma is a plasma reforming method, a silent discharge, partial discharge, creeping discharge, or corona discharge plasma, skin modification method.
21. The method of claim 16,

    Wherein said light is a laser.
22. The method of claim 21,

    Wherein said laser is a peelable laser.
23. The method of claim 21,

    Wherein said laser is a laser of low power laser therapy.
24. The method of claim 16,

    The light is characterized in that the wavelength of 605nm to 650nm, skin modification method.
25. The method of claim 16,

    Wherein said skin is a scalp.
26. The method of claim 25,

    Wherein said skin modification is to inhibit hair loss, promote hair growth, or strengthen hair roots.
27. The method of claim 16,

    Wherein the plasma is treated at 5 to 40 J / cm ² to the skin.
28. The method of claim 27,

    Wherein the plasma is treated at 10 to 20 J / cm ² on the skin.
29. The method of claim 16,

    Wherein said light is treated to the skin at 2 to 16 J / cm ² .
30. The method of claim 29,

    Wherein said light is treated to the skin at 2 to 8 J / cm ² .

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