CN110787374A

CN110787374A - Method for promoting hair regeneration based on plasma

Info

Publication number: CN110787374A
Application number: CN201810874911.5A
Authority: CN
Inventors: 吴耀炯; 张若兵; 孔德强; 徐国旺
Original assignee: Shenzhen Graduate School Tsinghua University
Current assignee: Shenzhen Graduate School Tsinghua University
Priority date: 2018-08-01
Filing date: 2018-08-01
Publication date: 2020-02-14

Abstract

The invention provides a method for promoting hair regeneration based on plasma, which comprises the following steps: the cold plasma is used for irradiating the skin to activate hair follicle stem cells and promote hair regeneration. Experiments prove that after the skin of a 7-8 week-old mouse in a resting period (follicle atrophy) is treated by the plasma jet, the atrophic follicles are immediately activated, so that the follicles are regenerated to form new follicles, and hairs grow out.

Description

Method for promoting hair regeneration based on plasma

Technical Field

The invention belongs to the field of regenerative medicine, and particularly relates to a method for promoting hair regeneration based on plasma.

Background

Plasma (Plasma) is a substance form which is generated by partial ionization of gas under high-voltage discharge and takes free electrons and charged ions as main components, is called a fourth form of the substance existing in a solid state, a liquid state and a gas state, the main active components of the substance form comprise a large number of active particles, such as electrons, ions, free radicals (ROS and RNS), molecules and electrically neutral atoms, the active components and the content of the Plasma jet can be different along with the change of parameters, such as the structure, the frequency, the voltage, the gas composition and the like of an ion source, and the composition and the content of the Plasma jet can also be different.

The plasma has strong inactivation effect on bacteria, and is mainly applied to sterilization and disinfection in the field of biology at first; with the progress of the plasma technology, particularly, the cold plasma has the characteristics of temperature close to room temperature, little thermal damage to tissues, environmental protection, little thermal damage to medical instruments, no thermal deformation of treatment materials and the like, so that the plasma can be rapidly applied to the aspects of material surface modification, wound healing, blood coagulation, tumor treatment and the like; in recent years, the research of plasma for regenerative medicine has received great attention, people try to use plasma to replace or assist medicines and traditional medical means to achieve better treatment effects, so that not only can the side effects of the medicines on human bodies be avoided, such as chemotherapy means, which bring great harm to people, but also the plasma is faster and more efficient. At present, related researches on the aspects of promoting chronic wound healing, blood coagulation, fracture healing and the like by applying plasma are internationally carried out, but related reports for promoting hair follicle regeneration and hair regeneration are not found.

Disclosure of Invention

It is an object of the present invention to provide a plasma-based method of promoting hair regrowth.

The invention provides a method for promoting hair regeneration based on plasma, which comprises the following steps: the plasma is used for irradiating the skin to activate hair follicle stem cells and promote hair regeneration.

In the above method, the plasma is a low temperature plasma jet (cold plasma jet).

The plasma directly irradiates a target area of skin.

The width of the plasma is 1mm-10cm, and specifically can be 5 mm.

The plasma takes inert gas as an ionized gas component, and the inert gas can be specifically helium, argon and the like.

The intensity (output voltage) of the plasma can be 2-10000V, specifically 3600V, the power supply voltage can be 2-220V, specifically 12V, the irradiation time can be 30 seconds-10 hours, specifically 1-30min, and the irradiation frequency can be: 1-100 times per treatment course, specifically comprising: the irradiation is carried out at 12V (power supply voltage) for 3min each time, 1 time daily, and 3 days continuously.

The use of plasma or plasma-generating devices for the preparation of products for promoting hair regrowth also falls within the scope of the present invention.

Experiments prove that after the skin of a 7-8 week-old mouse in a resting period (follicle atrophy) is treated by the plasma jet, the atrophic follicles are immediately activated, so that the follicles are regenerated to form new follicles, and hairs grow out.

Drawings

FIG. 1 is a diagram of experimental procedures and animal models.

Fig. 2 is a graph showing the results of hair regrowth after plasma treatment. A: before the experimental animal is treated; B. c, treatment of local skin darkening (appearance of hair follicles entering anagen phase) on day 10 after plasma treatment; D. f: on day 14 after plasma treatment, black hair was seen to grow out.

Fig. 3 is a diagram of skin histological analysis: after depilating for 2 weeks, taking skin tissues of treatment parts of the control group and the experimental group, slicing, carrying out HE staining, and observing under a microscope. A: the hair follicle of the control group skin tissue is in a resting stage; b: the plasma treated group skin follicles were in the growth phase.

Fig. 4 is a graph of plasma-enhanced hair follicle regeneration positively correlated with treatment time. A: control (left), plasma treatment for different time groups (right); b: HE staining of skin tissues of mice in a control group; C. d, E: HE staining of mouse skin tissue after plasma treatment for 1 min, 10 min, and 30min, respectively.

Fig. 5 is a schematic diagram of a self-sustaining cold plasma-activated hair follicle rejuvenating device.

Detailed Description

The present invention will be described below with reference to specific examples, but the present invention is not limited thereto.

The experimental methods used in the following examples are all conventional methods unless otherwise specified; reagents, materials and the like used in the following examples are commercially available unless otherwise specified.

The self-developed plasma apparatus (see fig. 5) used in the following examples mainly includes a power supply, a gas flow meter, a gas cylinder, a plasma nozzle, etc., wherein the gas flow rate can be controlled by the gas flow meter, the gas ejected from the gas cylinder is partially ionized in a high-voltage discharge to generate a large amount of plasma active substances, the main active components of which include a large amount of active particles, such as electrons, ions, radicals (ROS and RNS), molecules, and electrically neutral atoms, and the active components and the content of which vary with the experimental parameters.

Example 1

Experimental methods

7-8 week old C57Bl/6 mice (hair follicles in resting stage) were randomly divided into experimental and control groups of 6 mice each. The back of the mouse is depilated by depilatory cream, 4 areas with the diameter of 5mm are selected as treatment parts (see figure 1) at the symmetrical parts of the back of the mouse, in order to further ensure that the plasma flow does not spread to the areas outside the treatment parts, the skin of the non-treatment parts is shielded and protected by 3M films.

The treatment part of the experimental group is irradiated by a cold plasma flow with the width of 5mm, the plasma intensity (power voltage) is 12V, the irradiation time is 3 minutes each time, the irradiation is carried out for 1 time every day for 3 consecutive days, the cold plasma flow is generated by a plasma emission device (as shown in figure 5), the cold plasma flow has clear boundary and no scattered jet. The control group was subjected to the same procedure but without plasma irradiation.

The treated part is photographed before and after treatment, and the growth condition of the hair follicle is observed by comparison.

The skin at the treatment site was taken at different times after treatment for histological analysis and observation of hair follicle changes.

Results 1: after the 10 th day of plasma treatment, the skin at the plasma treatment part was found to have a phenomenon of hair regrowth, the skin became black, and after 2 weeks, hair was significantly grown; no hair growth occurred in the skin at sites other than the treatment site of the same mouse, i.e., the corresponding sites of the control mice (FIG. 2). Microscopic observation of the treated site skin tissue sections after HE staining showed that the 2-week hair follicles were in the anagen phase after plasma treatment, while the hair follicles in the non-treated site skin and control mouse skin were still in the resting phase (fig. 3).

Example 2 examination of the influence of irradiation intensity on the ability to promote regeneration

The hair regeneration was found to be associated with the plasma treatment time within a certain range, and the number of hair follicles regenerated was also significantly increased with the increase of the treatment time, but the ability to promote regeneration was somewhat decreased beyond a certain treatment time (fig. 4) after irradiation with a cold plasma stream having a width of 5mm, keeping the intensity of the plasma constant (power supply voltage 12 v), and irradiating for 1 minute, 10 minutes, and 30 minutes (three times correspond to C, D, E figure), 1 time per day, three consecutive days, and 10 days later.

Claims

1. A method of promoting hair regrowth comprising: the plasma is used for irradiating the skin to activate hair follicle stem cells and promote hair regeneration.

2. The method of claim 1, wherein: the plasma is a low temperature plasma jet, i.e. a cold plasma jet.

3. The method according to claim 1 or 2, characterized in that: the plasma directly irradiates a target area of skin.

4. The method according to any one of claims 1-3, wherein: the width of the plasma is between 1mm and 10 cm.

5. The method according to any one of claims 1-4, wherein: the plasma uses an inert gas as an ionized gas component.

6. The method of claim 5, wherein: the inert gas is helium or argon.

7. The method according to any one of claims 1-6, wherein: the intensity of the plasma is 2-10000V, the power voltage is 2-220V, the irradiation time is 30 seconds-10 hours each time, and the irradiation frequency is as follows: 1-100 times per treatment course.

8. Use of a plasma or plasma-generating device in the manufacture of a product for promoting hair regrowth.