WO2018074498A8 - リムルス属由来の組換え蛋白質及びこれをコードするｄｎａ - Google Patents

リムルス属由来の組換え蛋白質及びこれをコードするｄｎａ Download PDF

Info

Publication number
WO2018074498A8
WO2018074498A8 PCT/JP2017/037629 JP2017037629W WO2018074498A8 WO 2018074498 A8 WO2018074498 A8 WO 2018074498A8 JP 2017037629 W JP2017037629 W JP 2017037629W WO 2018074498 A8 WO2018074498 A8 WO 2018074498A8
Authority
WO
WIPO (PCT)
Prior art keywords
recombinant proteins
molecules encoding
dna molecules
proteins derived
encoding same
Prior art date
Application number
PCT/JP2017/037629
Other languages
English (en)
French (fr)
Other versions
WO2018074498A1 (ja
Inventor
光 水村
雄毅 小林
俊男 小田
Original Assignee
生化学工業株式会社
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 生化学工業株式会社 filed Critical 生化学工業株式会社
Priority to CN201780055566.6A priority Critical patent/CN109689681B/zh
Priority to EP17862926.7A priority patent/EP3530670B1/en
Priority to CN202210146889.9A priority patent/CN114507690A/zh
Priority to JP2018546373A priority patent/JP6927993B2/ja
Priority to EP24150705.2A priority patent/EP4339284A2/en
Priority to US16/331,810 priority patent/US20190241629A1/en
Publication of WO2018074498A1 publication Critical patent/WO2018074498A1/ja
Publication of WO2018074498A8 publication Critical patent/WO2018074498A8/ja

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/43504—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates
    • C07K14/43509—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates from crustaceans
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2477—Hemicellulases not provided in a preceding group
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/579—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving limulus lysate
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Zoology (AREA)
  • Urology & Nephrology (AREA)
  • Immunology (AREA)
  • Hematology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Toxicology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Insects & Arthropods (AREA)
  • Plant Pathology (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

リムルス・ポリフェムスの凝固メカニズムに関与するすべての完全長の組換え蛋白質、これらをコードするｃＤＮＡ、これらの用途を提供する。配列番号２または４で表されるアミノ酸配列を含む組換え蛋白質、配列番号６、８、１０、または１２で表されるアミノ酸配列を含む組換え蛋白質、配列番号１４、１６、１８、２０、または２２で表されるアミノ酸配列を含む組換え蛋白質、それらのバリアント、それらをコードするｃＤＮＡ、それらの利用。
PCT/JP2017/037629 2016-10-18 2017-10-18 リムルス属由来の組換え蛋白質及びこれをコードするｄｎａ WO2018074498A1 (ja)

Priority Applications (6)

Application Number Priority Date Filing Date Title
CN201780055566.6A CN109689681B (zh) 2016-10-18 2017-10-18 源自鲎属的重组蛋白及编码该重组蛋白的dna
EP17862926.7A EP3530670B1 (en) 2016-10-18 2017-10-18 Method for producing endotoxin detecting agent comprising recombinant limulus factor c and use thereof
CN202210146889.9A CN114507690A (zh) 2016-10-18 2017-10-18 源自鲎属的重组蛋白及编码该重组蛋白的dna
JP2018546373A JP6927993B2 (ja) 2016-10-18 2017-10-18 リムルス属由来の組換え蛋白質及びこれをコードするｄｎａ
EP24150705.2A EP4339284A2 (en) 2016-10-18 2017-10-18 Recombinant proteins derived from genus limulus, and dna molecules encoding same
US16/331,810 US20190241629A1 (en) 2016-10-18 2017-10-18 Recombinant proteins derived from genus limulus, and dna molecules encoding same

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2016-204729 2016-10-18
JP2016204729 2016-10-18

Publications (2)

Publication Number Publication Date
WO2018074498A1 WO2018074498A1 (ja) 2018-04-26
WO2018074498A8 true WO2018074498A8 (ja) 2018-08-30

Family

ID=62018519

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/JP2017/037629 WO2018074498A1 (ja) 2016-10-18 2017-10-18 リムルス属由来の組換え蛋白質及びこれをコードするｄｎａ

Country Status (5)

Country Link
US (1) US20190241629A1 (ja)
EP (2) EP4339284A2 (ja)
JP (1) JP6927993B2 (ja)
CN (2) CN109689681B (ja)
WO (1) WO2018074498A1 (ja)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP6785521B2 (ja) 2018-10-03 2020-11-18 生化学工業株式会社 エンドトキシン測定剤の感度の増強方法

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5716834A (en) 1994-08-19 1998-02-10 National University Of Singapore Cloned factor C cDNA of the Singapore horseshoe crab, Carcinoscorpius rotundicauda and purification of factor C proenzyme
US6645724B1 (en) 1997-09-19 2003-11-11 National University Of Singapore Assays for endotoxin
EP2050820A4 (en) 2006-07-07 2009-12-16 Seikagaku Kogyo Co Ltd PRO-COAGULATION ENZYME, AND METHOD FOR DETECTION OF ENDOTOXIN OR (1 3) -D-GLUCAN USING THE SAME
CN106432457A (zh) * 2011-02-28 2017-02-22 生化学工业株式会社 用于测量内毒素的试剂
JP2013124905A (ja) * 2011-12-14 2013-06-24 Katsuya Inada 血液エンドトキシン測定用試料作成方法
EP2740791A1 (en) * 2012-12-05 2014-06-11 MicroCoat Biotechnologie GmbH Method for recombinant production of horseshoe crab Factor C protein in protozoa
ES2738593T3 (es) * 2012-12-10 2020-01-23 Seikagaku Kogyo Co Ltd Factor C recombinante novedoso y método para producir el mismo, y método para medir endotoxina
JP2015141088A (ja) * 2014-01-28 2015-08-03 東亜ディーケーケー株式会社 高純度リムルス試薬、エンドトキシン濃度測定キット、及びエンドトキシン濃度測定法
JP6001131B1 (ja) 2015-04-28 2016-10-05 株式会社日立国際電気 基板処理装置、半導体装置の製造方法、プログラム
CA3054780A1 (en) * 2017-03-01 2018-09-07 Seikagaku Corporation Horseshoe crab factor b variant

Also Published As

Publication number Publication date
CN109689681A (zh) 2019-04-26
EP3530670A4 (en) 2020-05-20
EP3530670B1 (en) 2024-02-28
CN114507690A (zh) 2022-05-17
US20190241629A1 (en) 2019-08-08
JPWO2018074498A1 (ja) 2019-08-08
WO2018074498A1 (ja) 2018-04-26
EP3530670A1 (en) 2019-08-28
CN109689681B (zh) 2022-04-26
EP4339284A2 (en) 2024-03-20
JP6927993B2 (ja) 2021-09-01

Similar Documents

Publication Publication Date Title
PH12018502361A1 (en) Gdf15 fusion proteins and uses thereof
MX2020011257A (es) Ácidos nucleicos recombinantes que codifican una o más proteínas cosméticas para usos estéticos.
EA201792651A1 (ru) Аденовирусные полинуклеотиды и полипептиды
WO2015155686A3 (en) Methods and compositions for rna-directed repression of transcription using crispr-associated genes
MX2021012047A (es) Polipeptidos de fusion de serpina y metodos para utilizar los mismos.
WO2013003641A3 (en) Serpin fusion polypeptides and methods of use thereof
EP3797583A3 (en) Methods and compositions for gene expression in plants
CO6470863A2 (es) Mutantes fgf21 y usos de los mismos
PE20190420A1 (es) Proteinas f de prefusion del virus respiratorio sincicial (vrs) solubles y estabilizadas
WO2011036443A3 (en) Si protease c- terminal polypeptides and uses thereof for treating inflammation or coagulation disorders
EA201791238A1 (ru) Способы и композиции, в которых используются варианты полипептидов klotho
WO2014202622A3 (en) Rasamsonia gene and use thereof
WO2017025179A8 (en) A transglutamine tag for efficient site-specific bioconjugation
BR112017020308A2 (pt) udp-glicosiltransferases
MX2021000155A (es) Moleculas de proteina multifuncionales que comprenden decorina y su uso.
EA202190240A1 (ru) Варианты рекомбинантного белка
EP4269562A3 (en) Antigen binding molecules and methods of use thereof
BR112017001463A2 (pt) célula hospedeira melhorada para produzir proteínas
MY195726A (en) Steviol Glycoside Transport
WO2017060318A3 (en) Kaurenoic acid hydroxylases
MY195945A (en) Ras Protein Degradation Inducing Molecule and Pharmaceutical Composition
MX2021008832A (es) Composiciones y metodos para la administracion de polipeptidos cftr.
WO2018074498A8 (ja) リムルス属由来の組換え蛋白質及びこれをコードするｄｎａ
WO2015143512A3 (en) Enhancement of recombinant protein expression with copper
DE602005016417D1 (de) Neue polypeptide zur in-vintro-isolierung und vorbeugung von infektionen mit legionella pneumophila

Legal Events

Date Code Title Description
121 Ep: the epo has been informed by wipo that ep was designated in this application

Ref document number: 17862926

Country of ref document: EP

Kind code of ref document: A1

ENP Entry into the national phase

Ref document number: 2018546373

Country of ref document: JP

Kind code of ref document: A

NENP Non-entry into the national phase

Ref country code: DE

ENP Entry into the national phase

Ref document number: 2017862926

Country of ref document: EP

Effective date: 20190520