WO2017188426A1 - Aptamère améliorant l'activité de peroxydase - Google Patents

Aptamère améliorant l'activité de peroxydase Download PDF

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Publication number
WO2017188426A1
WO2017188426A1 PCT/JP2017/016884 JP2017016884W WO2017188426A1 WO 2017188426 A1 WO2017188426 A1 WO 2017188426A1 JP 2017016884 W JP2017016884 W JP 2017016884W WO 2017188426 A1 WO2017188426 A1 WO 2017188426A1
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WO
WIPO (PCT)
Prior art keywords
aptamer
peroxidase activity
ggg
heme protein
base sequence
Prior art date
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PCT/JP2017/016884
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English (en)
Japanese (ja)
Inventor
一典 池袋
恭子 山岸
真菜 金指
久野　斉
Original Assignee
国立大学法人東京農工大学
株式会社デンソー
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Publication date
Priority claimed from JP2017087665A external-priority patent/JP7012299B2/ja
Application filed by 国立大学法人東京農工大学, 株式会社デンソー filed Critical 国立大学法人東京農工大学
Publication of WO2017188426A1 publication Critical patent/WO2017188426A1/fr

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/115—Aptamers, i.e. nucleic acids binding a target molecule specifically and with high affinity without hybridising therewith ; Nucleic acids binding to non-nucleic acids, e.g. aptamers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids

Abstract

L'invention concerne un nouvel aptamère capable d'améliorer l'activité de peroxydase d'une protéine à hème et un procédé de quantification d'une protéine à hème à l'aide de l'aptamère. L'aptamère, qui comprend un polynucléotide présentant une séquence de base représentée par la formule [I] : ggg(n)1-2ggg(n)1-8ggg(n)1-2ggg [I] ou un polynucléotide contenant ledit polynucléotide, améliore l'activité de peroxydase d'une protéine à hème. Le procédé de quantification d'une protéine à hème dans un échantillon de test consiste à mesurer, en présence de cet aptamère, l'activité de peroxydase dans un échantillon de test contenant une protéine à hème prélevé sur un organisme vivant.
PCT/JP2017/016884 2016-04-28 2017-04-28 Aptamère améliorant l'activité de peroxydase WO2017188426A1 (fr)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
JP2016-091390 2016-04-28
JP2016091390 2016-04-28
JP2017-087665 2017-04-26
JP2017087665A JP7012299B2 (ja) 2016-04-28 2017-04-26 ペルオキシダーゼ活性増大アプタマー

Publications (1)

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WO2017188426A1 true WO2017188426A1 (fr) 2017-11-02

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ID=60159827

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/JP2017/016884 WO2017188426A1 (fr) 2016-04-28 2017-04-28 Aptamère améliorant l'activité de peroxydase

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WO (1) WO2017188426A1 (fr)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2021511795A (ja) * 2018-01-29 2021-05-13 ザ・ブロード・インスティテュート・インコーポレイテッド Ｃｒｉｓｐｒエフェクター系に基づく診断
WO2023191079A1 (fr) * 2022-04-01 2023-10-05 国立大学法人東京農工大学 Réactif et procédé de détection de substance cible
WO2024048599A1 (fr) * 2022-08-31 2024-03-07 国立大学法人東京農工大学 Complexe protéine-acide nucléique, kit de détection de substance cible et procédé de détection l'utilisant

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH04262796A (ja) * 1991-02-18 1992-09-18 Green Cross Corp:The 基質液の安定化法及び基質液
JPH0829430A (ja) * 1994-07-13 1996-02-02 Kyowa Hakko Kogyo Co Ltd ヘモグロビンの測定方法
WO2013005723A1 (fr) * 2011-07-04 2013-01-10 Ｎｅｃソフト株式会社 Procédé d'évaluation de l'activité d'oxydo-réduction d'une molécule d'acide nucléique, et molécule d'acide nucléique ayant une activité d'oxydo-réduction
WO2013140629A1 (fr) * 2012-03-23 2013-09-26 Ｎｅｃソフト株式会社 Dispositif d'analyse d'atp ou amp et procédé d'analyse
WO2013141291A1 (fr) * 2012-03-23 2013-09-26 Ｎｅｃソフト株式会社 Dispositif pour l'analyse cible de la streptavidine, et procédé d'analyse

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH04262796A (ja) * 1991-02-18 1992-09-18 Green Cross Corp:The 基質液の安定化法及び基質液
JPH0829430A (ja) * 1994-07-13 1996-02-02 Kyowa Hakko Kogyo Co Ltd ヘモグロビンの測定方法
WO2013005723A1 (fr) * 2011-07-04 2013-01-10 Ｎｅｃソフト株式会社 Procédé d'évaluation de l'activité d'oxydo-réduction d'une molécule d'acide nucléique, et molécule d'acide nucléique ayant une activité d'oxydo-réduction
WO2013140629A1 (fr) * 2012-03-23 2013-09-26 Ｎｅｃソフト株式会社 Dispositif d'analyse d'atp ou amp et procédé d'analyse
WO2013141291A1 (fr) * 2012-03-23 2013-09-26 Ｎｅｃソフト株式会社 Dispositif pour l'analyse cible de la streptavidine, et procédé d'analyse
WO2013140681A1 (fr) * 2012-03-23 2013-09-26 Ｎｅｃソフト株式会社 Dispositif pour analyse cible, et procédé d'analyse

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
ZHU, LING ET AL.: "In Vitro Selection of Highly Efficient G-Quadruplex-Based DNAzymes", ANAL. CHEM., vol. 84, no. 19, 2012, pages 8383 - 8390, XP055436522, ISSN: 1520-6882 *

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2021511795A (ja) * 2018-01-29 2021-05-13 ザ・ブロード・インスティテュート・インコーポレイテッド Ｃｒｉｓｐｒエフェクター系に基づく診断
WO2023191079A1 (fr) * 2022-04-01 2023-10-05 国立大学法人東京農工大学 Réactif et procédé de détection de substance cible
WO2024048599A1 (fr) * 2022-08-31 2024-03-07 国立大学法人東京農工大学 Complexe protéine-acide nucléique, kit de détection de substance cible et procédé de détection l'utilisant

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