WO2017176084A3 - 하수오 기내배양용 배지 조성물 및 이를 이용하는 종묘 대량 생산방법 - Google Patents

하수오 기내배양용 배지 조성물 및 이를 이용하는 종묘 대량 생산방법 Download PDF

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WO2017176084A3
WO2017176084A3 PCT/KR2017/003811 KR2017003811W WO2017176084A3 WO 2017176084 A3 WO2017176084 A3 WO 2017176084A3 KR 2017003811 W KR2017003811 W KR 2017003811W WO 2017176084 A3 WO2017176084 A3 WO 2017176084A3
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WIPO (PCT)
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polygoni multiflori
mass production
multiflori radix
vitro culturing
same
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PCT/KR2017/003811
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English (en)
French (fr)
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WO2017176084A2 (ko
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강영민
문병철
이가연
최지은
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한국 한의학 연구원
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Priority to CN201780022471.4A priority Critical patent/CN108882687A/zh
Publication of WO2017176084A2 publication Critical patent/WO2017176084A2/ko
Publication of WO2017176084A3 publication Critical patent/WO2017176084A3/ko

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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G22/00Cultivation of specific crops or plants not otherwise provided for
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01HNEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H4/00Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
    • A01H4/001Culture apparatus for tissue culture
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01HNEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H4/00Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0018Culture media for cell or tissue culture
    • C12N5/0025Culture media for plant cell or plant tissue culture
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00Specific components of cell culture medium
    • C12N2500/05Inorganic components
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00Specific components of cell culture medium
    • C12N2500/30Organic components
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00Active agents used in cell culture processes, e.g. differentation
    • C12N2501/30Hormones

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  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Botany (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Cell Biology (AREA)
  • Environmental Sciences (AREA)
  • Developmental Biology & Embryology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Cultivation Of Plants (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)

Abstract

본 발명은 하수오 기내배양용 배지 조성물 및 이를 이용하는 종묘 대량 생산방법에 관한 것이다. 상세하게는 10종의 배지를 사용하여 하수오 기내배양하여 생장율이 우수한 배지를 선정하고, 이를 기반으로 식물생장조절제로 키네틴과 IAA 또는 NAA를 이용하여 배양한 것이 덩이뿌리 분화가 우수하다는 것을 확인하였다. 또한 상기 배지 내의 무기염류의 함량을 조절하여 배양함으로써 덩이뿌리 분화가 우수한 배양묘를 생산하였고, 생산된 배양묘가 토양순화가 잘되는 것을 확인하였다.
PCT/KR2017/003811 2016-04-07 2017-04-07 하수오 기내배양용 배지 조성물 및 이를 이용하는 종묘 대량 생산방법 WO2017176084A2 (ko)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201780022471.4A CN108882687A (zh) 2016-04-07 2017-04-07 用于离体培养何首乌的培养基组合物及利用其大量生产种苗的方法

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
KR1020160043000A KR101777833B1 (ko) 2016-04-07 2016-04-07 하수오 기내배양용 배지 조성물 및 이를 이용하는 종묘 대량 생산방법
KR10-2016-0043000 2016-04-07

Publications (2)

Publication Number Publication Date
WO2017176084A2 WO2017176084A2 (ko) 2017-10-12
WO2017176084A3 true WO2017176084A3 (ko) 2018-01-18

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PCT/KR2017/003811 WO2017176084A2 (ko) 2016-04-07 2017-04-07 하수오 기내배양용 배지 조성물 및 이를 이용하는 종묘 대량 생산방법

Country Status (3)

Country Link
KR (1) KR101777833B1 (ko)
CN (1) CN108882687A (ko)
WO (1) WO2017176084A2 (ko)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR102321579B1 (ko) * 2019-09-30 2021-11-05 한국 한의학 연구원 Kiom 법하수오 추출물을 유효성분으로 포함하는 골다공증의 예방, 개선 또는 치료용 조성물

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20100114741A (ko) * 2009-04-16 2010-10-26 김상환 하수오 재배방법 및 그 방법으로 재배되는 하수오
KR20110113918A (ko) * 2010-04-12 2011-10-19 충청북도 (관리부서:충청북도 농업기술원) 정단배양 방법을 이용한 블루베리의 식물체 형성 방법
KR101211915B1 (ko) * 2010-12-24 2012-12-13 대한민국 고구마 조직배양묘의 증식을 위한 액체 배지 조성물 및 이를 이용한 증식방법

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1236670C (zh) * 2003-03-03 2006-01-18 北京葆圣庄植物科技有限公司 红栌高频率试管植株再生与工厂化育苗方法
CN100484393C (zh) * 2006-10-08 2009-05-06 南京林业大学 何首乌的组织培养快繁方法
CN100581353C (zh) * 2007-06-07 2010-01-20 株洲千金药业股份有限公司 芸香科花椒属植物单面针的人工快速繁殖方法
GB201210374D0 (en) * 2012-06-12 2012-07-25 New Energy Farms Plant propagation
CN103461126A (zh) * 2013-09-17 2013-12-25 南京通泽农业科技有限公司 一种榈木的快速繁殖方法

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20100114741A (ko) * 2009-04-16 2010-10-26 김상환 하수오 재배방법 및 그 방법으로 재배되는 하수오
KR20110113918A (ko) * 2010-04-12 2011-10-19 충청북도 (관리부서:충청북도 농업기술원) 정단배양 방법을 이용한 블루베리의 식물체 형성 방법
KR101211915B1 (ko) * 2010-12-24 2012-12-13 대한민국 고구마 조직배양묘의 증식을 위한 액체 배지 조성물 및 이를 이용한 증식방법

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Also Published As

Publication number Publication date
CN108882687A (zh) 2018-11-23
WO2017176084A2 (ko) 2017-10-12
KR101777833B1 (ko) 2017-09-13

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