WO2017170657A1 - ヨーグルトの製造方法 - Google Patents
ヨーグルトの製造方法 Download PDFInfo
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- WO2017170657A1 WO2017170657A1 PCT/JP2017/012838 JP2017012838W WO2017170657A1 WO 2017170657 A1 WO2017170657 A1 WO 2017170657A1 JP 2017012838 W JP2017012838 W JP 2017012838W WO 2017170657 A1 WO2017170657 A1 WO 2017170657A1
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- milk
- yogurt
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Images
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/78—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5)
- C12N9/80—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5) acting on amide bonds in linear amides (3.5.1)
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23C—DAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; MAKING THEREOF
- A23C9/00—Milk preparations; Milk powder or milk powder preparations
- A23C9/12—Fermented milk preparations; Treatment using microorganisms or enzymes
- A23C9/1203—Addition of, or treatment with, enzymes or microorganisms other than lactobacteriaceae
- A23C9/1209—Proteolytic or milk coagulating enzymes, e.g. trypsine
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23C—DAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; MAKING THEREOF
- A23C9/00—Milk preparations; Milk powder or milk powder preparations
- A23C9/12—Fermented milk preparations; Treatment using microorganisms or enzymes
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23C—DAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; MAKING THEREOF
- A23C9/00—Milk preparations; Milk powder or milk powder preparations
- A23C9/12—Fermented milk preparations; Treatment using microorganisms or enzymes
- A23C9/1203—Addition of, or treatment with, enzymes or microorganisms other than lactobacteriaceae
- A23C9/1216—Other enzymes
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23C—DAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; MAKING THEREOF
- A23C9/00—Milk preparations; Milk powder or milk powder preparations
- A23C9/12—Fermented milk preparations; Treatment using microorganisms or enzymes
- A23C9/123—Fermented milk preparations; Treatment using microorganisms or enzymes using only microorganisms of the genus lactobacteriaceae; Yoghurt
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23C—DAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; MAKING THEREOF
- A23C9/00—Milk preparations; Milk powder or milk powder preparations
- A23C9/12—Fermented milk preparations; Treatment using microorganisms or enzymes
- A23C9/13—Fermented milk preparations; Treatment using microorganisms or enzymes using additives
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23C—DAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; MAKING THEREOF
- A23C9/00—Milk preparations; Milk powder or milk powder preparations
- A23C9/12—Fermented milk preparations; Treatment using microorganisms or enzymes
- A23C9/13—Fermented milk preparations; Treatment using microorganisms or enzymes using additives
- A23C9/137—Thickening substances
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/125—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives containing carbohydrate syrups; containing sugars; containing sugar alcohols; containing starch hydrolysates
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/17—Amino acids, peptides or proteins
- A23L33/195—Proteins from microorganisms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Definitions
- the present invention relates to a method for producing yogurt by adding a specific enzyme and starch.
- Patent Document 1 a method for producing a cheese or yogurt with a smooth texture and suppressed acidity and bitterness by adding protein deamidase to raw milk is known.
- low protein yogurt is desired for healthy people who require a low protein diet in addition to low fat and patients with certain diseases.
- low-fat and low-protein yogurt has a problem in terms of shape retention because it has a low solid content and therefore water separation during storage increases.
- natural agar commonly used in yogurt and gums such as gelatin, carrageenan, and xanthan gum may be used. It is known to lack a feeling.
- An object of the present invention is to provide a method for producing a low-fat and low-protein yogurt that has a smooth mouthfeel, is suppressed in water separation, and has excellent shape retention.
- the inventors of the present invention have added a protein glutaminase and starch, so that it is smooth and palatable, water separation is suppressed, and shape retention is excellent. Has been found, and the present invention has been completed.
- the present invention is as follows.
- a method for producing yogurt comprising a step of adding protein glutaminase and a step of adding starch to raw milk.
- the raw milk is milk having a milk fat content of 1.5% by weight or less and a protein content of 5.5% by weight or less.
- the starch is a starch having an average particle diameter of 1 ⁇ m or more and less than 40 ⁇ m.
- [5] The method according to any one of [1] to [4], wherein the amount of protein glutaminase added is 0.05 unit or more and 100 units or less per gram of milk protein weight in the raw milk.
- [6] The method according to any one of [1] to [5], wherein the step of adding protein glutaminase is performed before the fermentation step.
- [7] The method according to any one of [1] to [6], wherein the step of adding starch is performed before the fermentation step.
- a yogurt having a smooth mouthfeel and a low calorie like a yogurt made from whole milk According to the present invention, a low-fat and low-protein yogurt with improved productivity and storage stability can be provided simply and at low cost.
- FIG. 1 shows the water separation rate of each yogurt.
- FIG. 2 shows the results of sensory evaluation of the smoothness of each yogurt.
- FIG. 3 shows the results of sensory evaluation of the viscosity feeling of each yogurt.
- the present invention relates to a method for producing yogurt including a step of adding protein glutaminase and a step of adding starch to raw milk (hereinafter sometimes abbreviated as “method of the present invention”).
- the method of the present invention is characterized in that it includes the above-mentioned two steps, but the other conventional steps such as the following can be applied.
- Raw material milk preparation step (2) Raw material milk sterilization step (3) Starter addition step (4) Fermentation step
- a protein glutaminase addition step and a starch addition step are added.
- the raw material milk in the present invention is milk of mammals such as cow's milk, goat's milk, skim milk, ingredient-prepared milk, processed milk, concentrated milk, diluted with water, dried ( Powdered milk), and dried powdered milk suspended and dissolved in water.
- the milk fat content in the raw milk in the present invention is usually 1.5% by weight or less, preferably 1% by weight or less, more preferably less than 0.5% by weight.
- the milk fat content may be 0, and is usually 0.1% by weight or more, preferably 0.3% by weight or more.
- the component standard of milk fat content of milk is 0.5% to 1.5% for low fat milk and less than 0.5% for non-fat milk.
- the protein content in the raw milk is usually 5.5% by weight or less, preferably 4% by weight or less, more preferably less than 4% by weight, and further preferably 3.5% by weight or less.
- the content is 0.5% by weight or more, preferably 1.5% by weight or more, and more preferably 2.5% by weight or more.
- non-fat milk As the raw material milk in the present invention, commercially available non-fat milk may be used, for example, a method of adjusting a conventional milk protein concentration by dissolving skim milk powder or the like in water and taking into account the protein amount of the skim milk powder. Accordingly, milk having milk fat content and protein adjusted within the above ranges may be used.
- Step of adding protein glutaminase may be carried out at any stage of a conventional production method of yogurt.
- the fermentation step Preferably after the fermentation step and before the fermentation step.
- the protein glutaminase used in the present invention may be a commercially available product or a protein glutaminase prepared from a culture solution of a microorganism that produces protein glutaminase.
- known protein separation and purification methods centrifugation, UF concentration, salting out, various chromatographies using ion exchange resins, etc.
- the target enzyme can be obtained by centrifuging the culture solution to remove the cells and then combining salting out, chromatography, and the like.
- the microbial cell is crushed by pressure treatment, ultrasonic treatment, etc., and then separated and purified in the same manner as described above to obtain the target enzyme.
- the enzyme may be pulverized by a drying method such as lyophilization or reduced pressure drying, and an appropriate excipient or drying aid may be used at that time.
- the type of protein glutaminase in the present invention is not particularly limited as long as it acts directly on the amide group of the protein and has a function of deamidation without peptide bond cleavage and protein cross-linking.
- Examples of such enzymes include proteins derived from the genus Chrysobacterium, Flavobacterium or Empedobacter disclosed in JP 2000-50887 A, JP 2001-218590 A, and WO 2006/075752.
- Glutaminase commercially available protein glutaminase derived from the genus Chrysobacterium, and the like, are not particularly limited thereto.
- the activity of protein glutaminase used in the present invention can be measured by the following method. (1) 0.1 ml of an aqueous solution containing protein glutaminase is added to 1 ml of 0.2 M phosphate buffer (pH 6.5) containing 30 mM Z-Gln-Gly, and incubated at 37 ° C. for 10 minutes, and then 0.4 M TCA The reaction is stopped by adding 1 ml of the solution.
- the amount of protein glutaminase added is usually 0.05 units or more, preferably 0.1 units or more, more preferably 0.5 units or more per gram of milk protein in the raw milk. Similarly, it is usually 100 units or less, preferably 25 units or less, and more preferably 10 units or less. If it is in said range, a smooth yogurt with a low water separation rate can be obtained.
- Step for adding Starch which is a feature of the present invention, may be performed at any stage of a conventional production method of yogurt. For example, before the fermentation step with lactic acid bacteria, etc., after the fermentation step Preferably, it is carried out before the fermentation step. Moreover, you may add before and after fermentation.
- the starch in the present invention is not particularly limited as long as it is a starch extracted from a plant and purified, and examples thereof include potatoes such as potato and sweet potato, cereals such as wheat, rice and corn, starches derived from vegetables, root vegetables and fruits. .
- potatoes such as potato and sweet potato
- cereals such as wheat, rice and corn
- potato and cereal starches are preferred, potato (potato), rice, wheat and corn are more preferred, rice, wheat and corn are more preferred, and rice and corn are particularly preferred.
- starch in the present invention is preferably starch that has not been pregelatinized ( ⁇ starch).
- yogurt can be provided not only to healthy individuals but also to patients who need a low GI (glycemic index) diet.
- the average particle diameter of starch is usually less than 40 ⁇ m from the viewpoint of smoothness and viscosity, preferably 30 ⁇ m or less, more preferably 20 ⁇ m or less, and even more preferably 15 ⁇ m or less. Moreover, from a viewpoint of the mixing property at the time of using as a formulation, it is 1 micrometer or more, and 2 micrometers or more are more preferable.
- the amount of starch added is usually 0.1% by weight or more, preferably 0.5% by weight or more, and more preferably 0.8% by weight or more based on the raw material milk. From the viewpoint of cost, it is 10% by weight or less, preferably 8% by weight or less, and more preferably 5% by weight or less.
- examples of the starter include lactic acid bacteria and yogurt (starter culture) in which live lactic acid bacteria remain.
- the addition amount of the starter can be determined by a conventional method depending on the type of raw material milk or starter.
- the addition amount of lactic acid bacteria starter for yogurt is usually 0.0001% to 1%, preferably 0.01% to 0.5%.
- the protein glutaminase and starch addition steps may be performed simultaneously or at a time difference.
- the protein glutaminase, starch and starter addition steps may be performed simultaneously or with a time lag.
- the temperature at which fermentation is performed by adding starch, protein glutaminase and starter to the raw milk for fermentation process is 0 to 80 ° C., preferably 3 to 50 ° C.
- the fermentation time is usually 1 to 8 hours, preferably 2 to 6 hours.
- the pH during fermentation is usually from 3.5 to 6.0, and when the pH reaches 4.0 to 5.0, the fermentation is terminated.
- yogurt in the present invention for example, a raw milk in which fat and protein raw materials are within the above-mentioned range is prepared, and if necessary, an additive such as sugar, flavor, etc. is added, Homogenize with a homogenizer. Then, after sterilizing and cooling by a conventional method, starch, protein glutaminase and starter (lactic acid bacteria, yogurt where live lactic acid bacteria remain, etc.) are added and fermented after filling the container, or in a tank after fermentation in the container Fill to produce yogurt. If necessary, when producing a stard-type yogurt, the obtained yogurt may be broken using a sieve (for example, a pore diameter of 500 ⁇ m) or a kitchen aid mixer.
- a sieve for example, a pore diameter of 500 ⁇ m
- the milk fat and protein content of the yogurt produced by the method of the present invention is the same as the milk fat and protein content of the raw milk since almost no change in milk fat and protein content due to lactic acid fermentation occurs.
- a low fat and low protein yoghurt is preferable, and the milk fat and protein content in the said yoghurt are 1.5 weight% or less normally and 5.5 weight% or less, respectively.
- the starch content of the yogurt obtained by the method of the present invention is usually 0.1 to 10% by weight, preferably 0.5 to 5% by weight.
- examples of the shape of yogurt in the present invention include solid, semi-solid, and liquid. Solid and semi-solid are preferable. Specific examples include set yogurt and stard yogurt.
- additives other than starch and protein glutaminase raw materials and additives usually used in the production of yogurt can be used.
- saccharides, stabilizers, emulsifiers, colorants, flavor modifiers, antioxidants and the like can be mentioned.
- Viscosity Evaluation The viscosity at 24 ° C. was measured using a dynamic viscoelasticity measuring device. The viscosity at 100 (1 / S) when the share rate was increased from 0 to 100 (1 / S) was measured. The results are shown in Tables 3 and 4.
- the obtained yogurt was subjected to the same test as the test methods 3 and 4 described above, and sensory evaluation was performed by three professional panels according to the criteria shown in Tables 1 and 2 above.
- Table 6 shows the average sensory evaluation of each paneler. Further, based on the test results, comprehensive evaluation was performed based on the above criteria.
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Abstract
Description
[1]原料乳に、プロテイングルタミナーゼを添加する工程及び澱粉を添加する工程を含むヨーグルトの製造方法。
[2]原料乳が、乳脂肪分含量が1.5重量%以下及びタンパク質含量が5.5重量%以下の乳である[1]に記載の方法。
[3]澱粉が、平均粒子径が1μm以上40μm未満の澱粉である[1]又は[2]に記載の方法。
[4]澱粉の添加量が、原料乳に対して0.1重量%以上10.0重量%以下である[1]~[3]のいずれかに記載の方法。
[5]プロテイングルタミナーゼの添加量が、原料乳中の乳タンパク質重量1gあたり0.05ユニット以上100ユニット以下である[1]~[4]のいずれかに記載の方法。
[6]プロテイングルタミナーゼを添加する工程が、発酵工程の前に行われる、[1]~[5]のいずれかに記載の方法。
[7]澱粉を添加する工程が、発酵工程の前に行われる、[1]~[6]のいずれかに記載の方法。
本発明によれば、製造性や保存安定性が向上した低脂肪・低タンパク質ヨーグルトを簡便にかつ低コストで提供することができる。
(1)原料乳の調製工程
(2)原料乳の殺菌工程
(3)スターターを添加する工程
(4)発酵工程
本発明においては、さらにプロテイングルタミナーゼを添加する工程及び澱粉を添加する工程が付加される。また上記以外にも冷却工程や他の添加物を加える工程を適宜含んでもよい。
本発明における原料乳は、牛乳、山羊乳等哺乳類の乳、それらの脱脂乳、成分調製乳、加工乳、それらを濃縮したもの、水で希釈したもの、乾燥させたもの(粉乳)、乾燥させた粉乳を水に懸濁、溶解したものが挙げられる。
本発明の特徴である「プロテイングルタミナーゼを添加する工程」は、ヨーグルトの慣用の製造方法のいずれの段階で行ってもよいが、例えば乳酸菌等による発酵工程の前、発酵工程の後に行われ、発酵工程の前に行われるのが好ましい。
(1)30mM Z-Gln-Glyを含む0.2Mリン酸バッファー(pH6.5)1mlにプロテイングルタミナーゼを含む水溶液0.1mlを添加して、37℃、10分間インキュベートした後、0.4M TCA溶液を1ml加えて反応を停止させる。ブランクとして、30mM Z-Gln-Glyを含む0.2Mリン酸バッファー(pH6.5)1mlと0.4M TCA溶液を1ml加えたものに、プロテイングルタミナーゼを含む水溶液0.1mlを添加したものを37℃で10分間インキュベートしたものを調製する。
(2)(1)の溶液についてアンモニアテストワコー(和光純薬)を用いて反応により生じたアンモニア量の測定を行う。アンモニア標準液(塩化アンモニウム)を用いて作成したアンモニア濃度と吸光度(630nm)の関係を表す検量線より、反応液中のアンモニア濃度を求める。
(3)プロテイングルタミナーゼの活性は、1分間に1μmolのアンモニアを生成する酵素量を1ユニットとし、所定の式から算出する。
本発明の特徴である「澱粉を添加する工程」は、ヨーグルトの慣用の製造方法のいずれの段階で行ってもよいが、例えば乳酸菌等による発酵工程の前、発酵工程の後に行われ、発酵工程の前に行われるのが好ましい。また発酵前と後に添加してもよい。
平均粒子径は、動的光散乱計測法で測定することができる。例えば、動的光散乱装置ゼータサイザーナノZS(Malvern社)を使用し、測定サンプルとしてMilliQ水にて10倍希釈した澱粉を800μlずつバイアルに充填し、オートサンプラーにて粒度分布及び平均粒子径を測定する(各試験区n=3)。
本発明の方法において、スターターとしては、乳酸菌、生きた乳酸菌が残存するヨーグルト(スターターカルチャー)が挙げられる。
スターターの添加量は、原料乳やスターターの種類によって慣用の方法によって決定することができる。例えば、ヨーグルト用乳酸菌スターター(クリスチャンハンセン社、YC-370)の添加量は、通常0.0001%~1%であり、0.01%~0.5%が好ましい。
原料乳に澱粉、プロテイングルタミナーゼ及びスターターを添加して発酵させるときの温度は0~80℃であり、好ましくは3~50℃である。また発酵時間は、通常1~8時間であり、好ましくは2~6時間である。また発酵時のpHは、通常pH3.5~6.0であり、pH4.0~5.0に達すると発酵を終了する。
また、これらの条件は、使用する酵素の純度やタンパク質の種類、純度などに応じて適宜変更ないし調整することができる。
また本発明の方法で得られたヨーグルトの澱粉含量は、通常0.1~10重量%、好ましくは0.5~5重量%である。
また本発明におけるヨーグルトの形状としては、固形、半固形、液状が挙げられるが、固形、半固形が好ましい。具体的には、セットヨーグルト、スタードヨーグルトなどが挙げられる。
スタードヨーグルトの調製
(1)原料としては、無脂肪乳(脂質0.1%、タンパク質3.5%;小岩井乳業株式会社)、ならびに牛乳(脂質0.1%、タンパク質3.8%;小岩井乳業株式会社)を使用する。各乳を300g測り取り、容器にいれて95℃で3分間加熱する。
(2)加熱後各乳を50℃まで氷水で冷やす。
(3)スターター(YC-370、クリスチャンハンセンジャパン株式会社)17.7mg/mL乳ならびに表3及び4に記載の割合でプロテイングルタミナーゼ(PG)(500U/g、天野エンザイム株式会社)及び各種澱粉を上記乳に添加する。
(4)44℃で4~5時間、pH4.6になるまでインキュベーターで発酵させる。
(5)発酵させてできたヨーグルトを20℃まで氷水で冷やす。
(6)篩(穴サイズ500μm)でヨーグルトを崩し(スタード)、十分冷蔵庫で冷やす。
離水率の測定
(1)離水測定のためカップ上にろ紙を敷き、20gのヨーグルトをろ紙上に置き20分間重力にて水を排出させる。
(2)カップにたまった水を測り取り、離水重量/ヨーグルト元重量を離水率(%)として計算する。結果は表3及び4ならびに図1に示した。
粘度評価
動的粘弾性測定装置を使用し24℃の時の粘度を測定した。0⇒100(1/S)までシェアレートを増加させた際の、100(1/S)になったときの粘度を測定した。結果は表3及び4に示した。
下記表1に示す基準に従い、ヨーグルトを食べた時のヨーグルトがざらつかず、口に残らない食感を「滑らかさ」として、7段階で3人の専門パネルによる官能評価を行った。各パネラーの官能評価の平均を表3及び4ならびに図2に示した。
ヨーグルトから感じる粘度の強さを「粘度感」として、下記表2に示す基準に従い、7段階で3人の専門パネルによる官能評価を行った。各パネラーの官能評価の平均を表3及び4ならびに図3に示す。
上記試験結果より、下記基準で総合評価を行った。
◎:滑らかさや粘度が高いヨーグルトであり最も好ましい。
○:滑らかさや粘度が適切なヨーグルトであり大変好ましい。
△:滑らかさや粘度が適切なヨーグルトであり好ましい。
×:ヨーグルトとして好ましくない。
セットヨーグルトの調製
(1)原料としては、無脂肪乳(脂質0.1%、タンパク質3.5%;小岩井乳業株式会社)を使用する。無脂肪乳を300g測り取り、容器にいれて95℃で3分間加熱する。
(2)加熱後無脂肪乳を50℃まで氷水で冷やす。
(3)スターター(YC-370、クリスチャンハンセンジャパン株式会社)17.7mg/mL無脂肪乳ならびに表6に記載の割合でプロテイングルタミナーゼ(PG)(500U/g、天野エンザイム株式会社)及び米澱粉(表5記載ファインスノウ)を上記無脂肪乳に添加する。
(4)得られた乳混合物をプラスチックカップへ40gずつ小分けし、44℃で4~5時間、pH4.6になるまでインキュベーターで発酵させる。
(5)発酵させてできたヨーグルトを冷蔵庫で冷やす。
Claims (7)
- 原料乳に、プロテイングルタミナーゼを添加する工程及び澱粉を添加する工程を含むヨーグルトの製造方法。
- 原料乳が、乳脂肪分含量が1.5重量%以下及びタンパク質含量が5.5重量%以下の乳である請求項1に記載の方法。
- 澱粉が、平均粒子径が1μm以上40μm未満の澱粉である請求項1又は2に記載の方法。
- 澱粉の添加量が、原料乳に対して0.1重量%以上10重量%以下である請求項1~3のいずれか1項に記載の方法。
- プロテイングルタミナーゼの添加量が、原料乳中の乳タンパク質重量1gあたり0.05ユニット以上100ユニット以下である請求項1~4のいずれか1項に記載の方法。
- プロテイングルタミナーゼを添加する工程が発酵工程の前に行われる、請求項1~5のいずれか1項に記載の方法。
- 澱粉を添加する工程が発酵工程の前に行われる、請求項1~6のいずれか1項に記載の方法。
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ES17775192T ES2899025T3 (es) | 2016-03-30 | 2017-03-29 | Método de producción de yogur |
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WO2023048195A1 (ja) * | 2021-09-21 | 2023-03-30 | 天野エンザイム株式会社 | タンパク質発酵飲食品の製造方法 |
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WO2021260067A2 (en) | 2020-06-24 | 2021-12-30 | Société des Produits Nestlé S.A. | Method for the treatment of protein-containing compositions |
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WO2022039281A1 (en) * | 2020-08-19 | 2022-02-24 | Ajinomoto Co., Inc. | Methods for producing stirred yogurt |
WO2023048195A1 (ja) * | 2021-09-21 | 2023-03-30 | 天野エンザイム株式会社 | タンパク質発酵飲食品の製造方法 |
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EP3437481A1 (en) | 2019-02-06 |
BR112018069455A2 (pt) | 2019-02-05 |
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