WO2017088144A1 - 用于白菜种质资源多样性分析及分子育种的snp组合及其应用 - Google Patents

用于白菜种质资源多样性分析及分子育种的snp组合及其应用 Download PDF

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WO2017088144A1
WO2017088144A1 PCT/CN2015/095671 CN2015095671W WO2017088144A1 WO 2017088144 A1 WO2017088144 A1 WO 2017088144A1 CN 2015095671 W CN2015095671 W CN 2015095671W WO 2017088144 A1 WO2017088144 A1 WO 2017088144A1
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chromosome
base
sequence
cabbage
snp
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PCT/CN2015/095671
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English (en)
French (fr)
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苏同兵
于拴仓
张凤兰
余阳俊
张德双
赵岫云
汪维红
卢桂香
隋光磊
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北京市农林科学院
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Priority to PCT/CN2015/095671 priority Critical patent/WO2017088144A1/zh
Priority to CN201580030487.0A priority patent/CN106460063B/zh
Publication of WO2017088144A1 publication Critical patent/WO2017088144A1/zh

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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/6895—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for plants, fungi or algae
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/156—Polymorphic or mutational markers

Definitions

  • the invention belongs to the field of molecular biology and relates to a SNP combination for the diversity analysis and molecular breeding of Chinese cabbage germplasm resources and its application.
  • China is the world's largest vegetable production and consumption country, while cabbage (Brassica rapa L.ssp. Pekinensis) is the largest vegetable crop in China, and plays an important role in the vegetable basket project.
  • cabbage is a hybrid crop, it has a high recombination rate, rich genetic diversity, and a good foundation for genomics research, and has become a model plant for genetic research.
  • China is the country with the most abundant germplasm resources of cabbage crops in the world.
  • Chinese cabbage, moss, rapeseed, turnip and oil cabbage Widely distributed in various regions of China. These types have significant differences in morphology compared to ordinary ball/non-heading Chinese cabbage, but their edible and commercial value is very high and are important breeding materials.
  • Hybrids are the dominant breeding methods for cabbage crops. Correct selection of parents and rational combination are the key to the success of breeding. In addition, the introduction of exogenous genetic material through hybridization and backcrossing is an important way to expand genetic variation. However, there is a common problem in the above process that needs to be solved: whether it is hybridization or backcrossing, both parents and donor parents have genes that are beneficial to crop improvement, and there are also a large number of genes that are not good for crop improvement. Accurate and effective identification methods are used to select the new germplasm obtained to ensure that new germplasm with exogenous beneficial genes and similar overall background to the target parent are obtained as much as possible.
  • SNPline genotyping is a high-throughput SNP typing technology developed by LGC (Laboratory of the Government Chemist). It has the characteristics of accuracy, flexibility, low cost and high throughput. It has become internationally.
  • LGC Laboratory of the Government Chemist
  • the object of the present invention is to provide a SNP combination, a primer combination and its application in breeding for genetic diversity analysis and molecular breeding of Chinese cabbage.
  • the application provided by the present invention specifically refers to the application of 571 SNP sites or substances for detecting the 571 SNP sites in any of the following:
  • the physical location of the 571 SNP loci is determined based on the genome-wide sequence alignment of the Chinese cabbage variety Chiifu-402-2, and the version number of the whole genome sequence of the Chinese cabbage variety Chiifu-402-2 is V1.5 (download The address is http://brassicadb.org/brad); the 571 SNP sites are BrSNPA01001-BrSNPA10043 (see Example 1 for details).
  • the "substance for detecting the 571 SNP sites” may be any reagent, instrument, device or the like capable of detecting the genotype of the 571 SNP sites.
  • the “substance for detecting the 571 SNP sites” is specifically 571 sets of primers for detecting the 571 SNP sites, and each set of primers is used for detecting the 571 SNP positions.
  • each set of primers of the 571 set of primers is composed of two upstream primers and one downstream primer respectively recorded as upstream primer 1 and upstream primer 2;
  • the structural composition of the upstream primer 1 is from the 5' end To the 3' end are: universal sequence 1, genomic specific sequence, one of two nucleotides at the SNP site;
  • the structural composition of the upstream primer 2 is from the 5' end to the 3' end:
  • the universal sequence 2 the genomic specific sequence, and the other of the two nucleotides at the SNP site;
  • the downstream primer consists only of genomic specific sequences.
  • the genome-specific sequence in all of the upstream primers is 1142 sequences obtained by removing all of the sequence X in the sequence listing from position 1-21; all of the downstream primers
  • the nucleotide sequence is all sequences Y in the sequence listing; wherein X is 3N-2 or 3N-1, Y is 3N, and N is an integer greater than or equal to 1 and less than or equal to 571. That is, there are a total of 1142 sequences n in the sequence table (n is a positive integer that is not a multiple of 3 in 1-1713, such as 1, 2, 4, 5, 7, 8, ... 1711, 1712).
  • the resulting 1142 sequences are the genome-specific sequences in all of the upstream primers; a total of 571 sequences in the sequence listing m (m is a positive integer that is a multiple of 3 in 1-1713, such as 3, 6, 9, ..., 1713) is all of the downstream primers.
  • nucleotide sequence of the universal sequence 1 is as shown in the first to first sequence of the sequence 1 in the sequence listing; the nucleotide sequence of the universal sequence 2 is as shown in the first and second sequences of the sequence 2 in the sequence listing. .
  • the 571 sets of primers are represented by Sequence 1 - Sequence 1713 in the Sequence Listing.
  • the three primers shown in 1-3 were used to detect the SNP site BrSNPA01001 (the first of the 571 SNP sites in Example 1); the three primers shown in the sequence 4-6 were used to detect the SNP Site BrSNPA01002 (the second of the 571 SNP sites in Example 1); the three primers shown in SEQ ID NO: 7-9 were used to detect the SNP site BrSNPA01003 (the first of the 571 SNP sites in Example 1) 3); ..., and so on, ..., the 3 primers shown in the sequence 1711-1713 were used to detect the SNP site BrSNPA10043 (the 571th of the 571 SNP sites in Example 1).
  • the inbred line material (donor) with excellent traits and the recurrent parent (receptor) with better comprehensive traits are usually multi-generation.
  • Backcrossing thereby obtaining a holistic trait similar to that of a recurrent parent, but increasing the offspring of favorable traits transferred from the donor.
  • the 571 SNP loci can be used to select the intermediate materials in the backcross breeding process to achieve the goal of quickly obtaining a new line of target traits.
  • the invention also provides a method for constructing a database of cabbage DNA fingerprints.
  • the method for constructing a cabbage DNA fingerprint database may specifically include the following steps:
  • the "substance for detecting the 571 SNP sites" as described above is used to detect the 571 SNP sites in the genomic DNA of all cabbage varieties. Genotype.
  • the invention also provides a method for classifying the germplasm resources of Chinese cabbage.
  • the method for classifying the germplasm resources of Chinese cabbage provided by the present invention may specifically include the following steps:
  • the "substance for detecting the 571 SNP sites" as described above is used to detect the 571 SNP sites in the genomic DNA of all cabbage varieties. Genotype.
  • the “establishing a clustering tree diagram (such as the UPGMA clustering tree diagram according to the obtained genotype data)” is specifically: using the Power-Marker ver.3.25 software according to the obtained genotype data.
  • the Rogers 1972 genetic distance calculation method was used to analyze and establish the UPGMA clustering tree diagram.
  • whether the cabbage variety to be tested is a new germplasm material can be determined according to the cabbage DNA fingerprint database constructed by the method provided by the present invention and/or the cluster tree diagram of the established Chinese cabbage germplasm resources.
  • the invention also provides a method for molecular breeding of Chinese cabbage.
  • the method for molecular breeding of Chinese cabbage may specifically comprise the steps of: hybridizing a cabbage donor parent carrying a target trait with a cabbage receptor parent (having a better comprehensive trait), and then obtaining the resulting hybrid progeny Performing a number of backcrosses with the cabbage donor parent, respectively detecting the genotype of the cabbage receptor parent and the obtained 571 SNP sites in the backcross progeny genomic DNA carrying the target trait, according to the detection result An individual having the highest genotype of the 571 SNP sites and the cabbage parent parent is selected from the backcross progeny carrying the target trait.
  • the invention also provides a product for detecting the genotype of the 571 SNP sites.
  • the product for detecting the genotype of the 571 SNP sites contains 571 sets of primers as described above for detecting the 571 SNP sites.
  • the cabbage may be Brassica rapa ssp. Pekinensis, Brassica rapa ssp. Chinensis, Brassica rapa ssp. oleifera (O) and Brassica rapa ssp. rapifera (R) Specifically; it may be selected from all or part of the varieties shown in Table 1 below.
  • Figure 1 shows that 571 SNP sites have good polymorphism in the 255 cabbage materials tested (Table 1).
  • A is the average polymorphic information content (PIC);
  • B is the minimum allele frequency MAF;
  • C is the heterozygosity (H o );
  • D is the average genetic diversity (GeneDiversity).
  • Example 2 is a genotypic data of 255 cabbage samples based on 255 cabbage samples (Table 1) obtained in Example 3.
  • Figure 3 is a population structure analysis of 255 cabbage materials (Table 1). Among them, A is to genotype the test sample by GGT2.0 software; B is to cluster the test sample by Power Marker and MEGA6.0; C is a photo of the relevant test sample. In the figure, 303# means "Jin Green No. 6"; 32# means "Jiao Erye”.
  • each locus was screened and evaluated according to the following characteristics: (a) uniform distribution of genome; (b) SNP isolocation The degree of homozygosity is >95%; (c), the minimum allele frequency is between 0.1 and 0.5; (d), the resequencing depth is >5 ⁇ . Finally, 571 SNP sites for DNA fingerprint database construction, variety authenticity detection, genetic resource analysis and molecular breeding were obtained.
  • the version number of the whole genome sequence of the Chinese cabbage variety Chiifu-401-42 is V1.5;
  • the 571 SNP sites are specifically as follows: BrSNPA01001-BrSNPA10043:
  • BrSNPA01001 is located at position 612144 on chromosome A01, and its base is T or A;
  • BrSNPA01002 is located at position 1059908 on chromosome A01, and its base is T or C;
  • BrSNPA01003 is located at position 1248141 on chromosome A01, and its base is T or C;
  • BrSNPA01004 is located at position 1385646 on chromosome A01, and its base is T or A;
  • BrSNPA01005 is located at position 1795429 on chromosome A01, and its base is T or C;
  • BrSNPA01006 is located at position 2112068 on chromosome A01, and its base is A or T;
  • BrSNPA01007 is located at position 2437505 on chromosome A01, and its base is G or A;
  • BrSNPA01008 is located at position 2830301 on chromosome A01, and its base is G or A;
  • BrSNPA01009 is located at position 2969334 on chromosome A01, and its base is G or A;
  • BrSNPA01010 is located at position 2970196 on chromosome A01, and its base is T or C;
  • BrSNPA01011 is located at position 3520140 on chromosome A01, and its base is T or C;
  • BrSNPA01012 is located at position 4182701 on chromosome A01, and its base is T or C;
  • BrSNPA01013 is located at position 4219165 on chromosome A01, and its base is C or A;
  • BrSNPA01014 is located at position 4225329 on chromosome A01, and its base is T or G;
  • BrSNPA01015 is located at position 4878177 on chromosome A01, and its base is T or C;
  • BrSNPA01016 is located at position 5849627 on chromosome A01, and its base is T or G;
  • BrSNPA01017 is located at position 6000315 on chromosome A01, and its base is C or G;
  • BrSNPA01018 is located at position 6385755 on chromosome A01, and its base is T or C;
  • BrSNPA01019 is located at position 7102966 on chromosome A01, and its base is T or A;
  • BrSNPA01020 is located at position 7105232 on chromosome A01, and its base is G or A;
  • BrSNPA01021 is located at position 7567812 on chromosome A01, and its base is T or C;
  • BrSNPA01022 is located at position 8475689 on chromosome A01, and its base is C or T;
  • BrSNPA01023 is located at position 9716212 on chromosome A01, and its base is G or C;
  • BrSNPA01024 is located at position 9865712 on chromosome A01, and its base is T or A;
  • BrSNPA01025 is located at position 10433882 on chromosome A01, and its base is A or G;
  • BrSNPA01026 is located at position 10454017 on chromosome A01, and its base is T or C;
  • BrSNPA01027 is located at position 11039761 on chromosome A01, and its base is T or C;
  • BrSNPA01028 is located at position 11576989 on chromosome A01, and its base is G or A;
  • BrSNPA01029 is located at position 12681321 on chromosome A01, and its base is T or G;
  • BrSNPA01030 is located at position 12708200 on chromosome A01, and its base is T or C;
  • BrSNPA01031 is located at position 12834470 on chromosome A01, and its base is G or C;
  • BrSNPA01032 is located at position 13128980 on chromosome A01, and its base is T or C;
  • BrSNPA01033 is located at position 14048162 on chromosome A01, and its base is T or C;
  • BrSNPA01034 is located at position 14897192 on chromosome A01, and its base is C or T;
  • BrSNPA01035 is located at position 15956879 on chromosome A01, and its base is T or C;
  • BrSNPA01036 is located at position 16592150 on chromosome A01, and its base is G or C;
  • BrSNPA01037 is located at position 17311260 on chromosome A01, and its base is T or C;
  • BrSNPA01038 is located at position 18199499 on chromosome A01, and its base is T or C;
  • BrSNPA01039 is located at position 18291203 on chromosome A01, and its base is T or C;
  • BrSNPA01040 is located at position 18635734 on chromosome A01, and its base is G or A;
  • BrSNPA01041 is located at position 18650317 on chromosome A01, and its base is G or A;
  • BrSNPA01042 is located at chromosome 1845725 on chromosome A01, and its base is T or C;
  • BrSNPA01043 is located at position 18964955 on chromosome A01, and its base is G or C;
  • BrSNPA01044 is located at position 20057539 on chromosome A01, and its base is G or A;
  • BrSNPA01045 is located at position 21096109 on chromosome A01, and its base is C or A;
  • BrSNPA01046 is located at position 21986324 on chromosome A01, and its base is G or A;
  • BrSNPA01047 is located at position 22365926 on chromosome A01, and its base is G or C;
  • BrSNPA01048 is located at position 22417219 on chromosome A01, and its base is G or A;
  • BrSNPA01049 is located at position 22449302 on chromosome A01, and its base is G or A;
  • BrSNPA01050 is located at position 22532311 on chromosome A01, and its base is C or T;
  • BrSNPA01051 is located at position 22600539 on chromosome A01, and its base is T or C;
  • BrSNPA01052 is located at position 22695943 on chromosome A01, and its base is C or A;
  • BrSNPA01053 is located at position 24361302 on chromosome A01, and its base is T or C;
  • BrSNPA01054 is located at position 24456539 on chromosome A01, and its base is G or T;
  • BrSNPA01055 is located at position 24937773 on chromosome A01, and its base is G or A;
  • BrSNPA01056 is located at position 25108874 on chromosome A01, and its base is T or C;
  • BrSNPA01057 is located at position 26035077 on chromosome A01, and its base is T or A;
  • BrSNPA01058 is located at position 26549708 on chromosome A01, and its base is C or T;
  • BrSNPA01059 is located at position 26752589 on chromosome A01, and its base is G or A;
  • BrSNPA02001 is located at position 356794 on chromosome A02, and its base is C or T;
  • BrSNPA02002 is located at position 751652 on chromosome A02, and its base is G or A;
  • BrSNPA02003 is located at position 884677 on chromosome A02, and its base is T or A;
  • BrSNPA02004 is located at position 1026373 on chromosome A02, and its base is T or C;
  • BrSNPA02005 is located at position 1065889 on chromosome A02, and its base is T or A;
  • BrSNPA02006 is located at position 1142477 on chromosome A02, and its base is T or C;
  • BrSNPA02007 is located at position 2421548 on chromosome A02, and its base is A or C;
  • BrSNPA02008 is located at position 2663267 on chromosome A02, and its base is T or C;
  • BrSNPA02009 is located at position 2865799 on chromosome A02, and its base is T or C;
  • BrSNPA02010 is located at position 3245711 on chromosome A02, and its base is C or A;
  • BrSNPA02011 is located at chromosome 3653520 on chromosome A02, and its base is G or A;
  • BrSNPA02012 is located at position 3812885 on chromosome A02, and its base is T or G;
  • BrSNPA02013 is located at position 4523362 on chromosome A02, and its base is T or A;
  • BrSNPA02014 is located at position 5184035 on chromosome A02, and its base is T or C;
  • BrSNPA02015 is located at position 6035955 on chromosome A02, and its base is T or G;
  • BrSNPA02016 is located at position 6411221 on chromosome A02, and its base is A or C;
  • BrSNPA02017 is located at position 6790555 on chromosome A02, and its base is C or A;
  • BrSNPA02018 is located at position 6957629 on chromosome A02, and its base is G or A;
  • BrSNPA02019 is located at position 8447338 on chromosome A02, and its base is T or C;
  • BrSNPA02020 is located at position 9141887 on chromosome A02, and its base is G or C;
  • BrSNPA02021 is located at position 9423555 on chromosome A02, and its base is T or C;
  • BrSNPA02022 is located at position 9490758 on chromosome A02, and its base is G or A;
  • BrSNPA02023 is located at position 11160802 on chromosome A02, and its base is C or A;
  • BrSNPA02024 is located at position 11181828 on chromosome A02, and its base is T or A;
  • BrSNPA02025 is located at position 11698932 on chromosome A02, and its base is T or A;
  • BrSNPA02026 is located at position 11780457 on chromosome A02, and its base is T or A;
  • BrSNPA02027 is located at position 13059562 on chromosome A02, and its base is G or A;
  • BrSNPA02028 is located at position 13619247 on chromosome A02, and its base is G or C;
  • BrSNPA02029 is located at position 13349430 on chromosome A02, and its base is T or A;
  • BrSNPA02030 is located at position 14606869 on chromosome A02, and its base is T or C;
  • BrSNPA02031 is located at position 14862673 on chromosome A02, and its base is T or C;
  • BrSNPA02032 is located at position 1537536 of chromosome A02, and its base is G or A;
  • BrSNPA02033 is located at position 16460513 on chromosome A02, and its base is G or C;
  • BrSNPA02034 is located at position 17440083 on chromosome A02, and its base is T or C;
  • BrSNPA02035 is located at position 17778839 on chromosome A02, and its base is G or A;
  • BrSNPA02036 is located at position 17828400 on chromosome A02, and its base is T or C;
  • BrSNPA02037 is located at position 17930830 on chromosome A02, and its base is T or C;
  • BrSNPA02038 is located at position 17961160 on chromosome A02, and its base is G or A;
  • BrSNPA02039 is located at position 18267114 on chromosome A02, and its base is T or C;
  • BrSNPA02040 is located at position 18374714 on chromosome A02, and its base is G or A;
  • BrSNPA02041 is located at position 18365894 on chromosome A02, and its base is T or G;
  • BrSNPA02042 is located at position 18425637 on chromosome A02, and its base is G or C;
  • BrSNPA02043 is located at position 19966721 on chromosome A02, and its base is T or A;
  • BrSNPA02044 is located at position 21426261 on chromosome A02, and its base is G or A;
  • BrSNPA02045 is located at position 21512340 on chromosome A02, and its base is G or A;
  • BrSNPA02046 is located at position 21970335 on chromosome A02, and its base is C or T;
  • BrSNPA02047 is located at position 22610904 on chromosome A02, and its base is T or C;
  • BrSNPA02048 is located at position 22844235 on chromosome A02, and its base is T or G;
  • BrSNPA02049 is located at position 22749952 on chromosome A02, and its base is G or A;
  • BrSNPA02050 is located at position 23028483 on chromosome A02, and its base is G or A;
  • BrSNPA02051 is located at position 23096858 on chromosome A02, and its base is T or C;
  • BrSNPA02052 is located at position 23174443 on chromosome A02, and its base is C or A;
  • BrSNPA02053 is located at position 23181920 on chromosome A02, and its base is G or C;
  • BrSNPA02054 is located at position 23632523 on chromosome A02, and its base is G or A;
  • BrSNPA02055 is located at position 24627499 on chromosome A02, and its base is A or G;
  • BrSNPA02056 is located at position 24987897 on chromosome A02, and its base is C or T;
  • BrSNPA02057 is located at position 25360564 on chromosome A02, and its base is C or A;
  • BrSNPA02058 is located at position 25748039 on chromosome A02, and its base is G or A;
  • BrSNPA02059 is located at position 26812976 on chromosome A02, and its base is G or C;
  • BrSNPA03001 is located at position 128271 on chromosome A03, and its base is C or A;
  • BrSNPA03002 is located at position 1038560 on chromosome A03, and its base is T or A;
  • BrSNPA03003 is located at position 1113130 on chromosome A03, and its base is T or C;
  • BrSNPA03004 is located at position 1964460 on chromosome A03, and its base is G or A;
  • BrSNPA03005 is located at position 2531794 on chromosome A03, and its base is T or C;
  • BrSNPA03006 is located at position 2904270 on chromosome A03, and its base is T or G;
  • BrSNPA03007 is located at position 3151130 on chromosome A03, and its base is G or A;
  • BrSNPA03008 is located at position 3151142 on chromosome A03, and its base is T or C;
  • BrSNPA03009 is located at position 3230367 on chromosome A03, and its base is T or C;
  • BrSNPA03010 is located at position 3596283 on chromosome A03, and its base is C or G;
  • BrSNPA03011 is located at position 3671765 on chromosome A03, and its base is T or C;
  • BrSNPA03012 is located at position 4366708 on chromosome A03, and its base is C or A;
  • BrSNPA03013 is located at position 4417310 on chromosome A03, and its base is T or G;
  • BrSNPA03014 is located at position 4471683 on chromosome A03, and its base is G or A;
  • BrSNPA03015 is located at position 5211812 on chromosome A03, and its base is A or T;
  • BrSNPA03016 is located at position 5450320 on chromosome A03, and its base is C or A;
  • BrSNPA03017 is located at position 6355471 on chromosome A03, and its base is G or A;
  • BrSNPA03018 is located at position 7120485 on chromosome A03, and its base is T or C;
  • BrSNPA03019 is located at position 7461075 on chromosome A03, and its base is G or C;
  • BrSNPA03020 is located at position 7482663 on chromosome A03, and its base is T or C;
  • BrSNPA03021 is located at position 7645227 on chromosome A03, and its base is T or C;
  • BrSNPA03022 is located at position 78284461 on chromosome A03, and its base is G or A;
  • BrSNPA03023 is located at position 7962179 on chromosome A03, and its base is G or A;
  • BrSNPA03024 is located at the 8473550 position on chromosome A03, and its base is G or A;
  • BrSNPA03025 is located at position 8903849 on chromosome A03, and its base is T or A;
  • BrSNPA03026 is located at position 8939875 on chromosome A03, and its base is T or C;
  • BrSNPA03027 is located at position 9140011 on chromosome A03, and its base is G or A;
  • BrSNPA03028 is located at position 9143142 on chromosome A03, and its base is C or A;
  • BrSNPA03029 is located at position 10375219 on chromosome A03, and its base is T or G;
  • BrSNPA03030 is located at position 10697749 on chromosome A03, and its base is A or G;
  • BrSNPA03031 is located at position 10918916 on chromosome A03, and its base is T or G;
  • BrSNPA03032 is located at position 11197981 on chromosome A03, and its base is G or C;
  • BrSNPA03033 is located at position 11383704 on chromosome A03, and its base is T or C;
  • BrSNPA03034 is located at position 11557175 on chromosome A03, and its base is C or T;
  • BrSNPA03035 is located at position 11754718 on chromosome A03, and its base is G or A;
  • BrSNPA03036 is located at position 12070633 on chromosome A03, and its base is G or A;
  • BrSNPA03037 is located at position 12523505 on chromosome A03, and its base is G or C;
  • BrSNPA03038 is located at position 12806173 on chromosome A03, and its base is T or A;
  • BrSNPA03039 is located at position 13329725 on chromosome A03, and its base is G or A;
  • BrSNPA03040 is located at position 14206073 on chromosome A03, and its base is C or T;
  • BrSNPA03041 is located at position 14660690 on chromosome A03, and its base is G or A;
  • BrSNPA03042 is located at position 14844334 on chromosome A03, and its base is G or C;
  • BrSNPA03043 is located at position 15312198 on chromosome A03, and its base is T or G;
  • BrSNPA03044 is located at position 15431305 on chromosome A03, and its base is G or C;
  • BrSNPA03045 is located at position 16097389 on chromosome A03, and its base is G or A;
  • BrSNPA03046 is located at position 16144120 on chromosome A03, and its base is G or C;
  • BrSNPA03047 is located at position 17940082 on chromosome A03, and its base is T or G;
  • BrSNPA03048 is located at position 18277222 on chromosome A03, and its base is T or A;
  • BrSNPA03049 is located at chromosome 1969018 on chromosome A03, and its base is A or G;
  • BrSNPA03050 is located at position 1934725 on chromosome A03, and its base is T or A;
  • BrSNPA03051 is located at position 19973528 on chromosome A03, and its base is T or C;
  • BrSNPA03052 is located at position 20233641 on chromosome A03, and its base is T or G;
  • BrSNPA03053 is located at position 20593495 on chromosome A03, and its base is A or T;
  • BrSNPA03054 is located at position 21003111 on chromosome A03, and its base is G or C;
  • BrSNPA03055 is located at position 21624403 on chromosome A03, and its base is T or G;
  • BrSNPA03056 is located at position 22632354 on chromosome A03, and its base is G or A;
  • BrSNPA03057 is located at position 23328550 on chromosome A03, and its base is C or A;
  • BrSNPA03058 is located at position 23335563 on chromosome A03, and its base is T or C;
  • BrSNPA03059 is located at position 23890389 on chromosome A03, and its base is A or G;
  • BrSNPA03060 is located at position 24086704 on chromosome A03, and its base is G or A;
  • BrSNPA03061 is located at position 24127049 on chromosome A03, and its base is T or A;
  • BrSNPA03062 is located at position 24352728 on chromosome A03, and its base is T or A;
  • BrSNPA03063 is located at position 25697075 on chromosome A03, and its base is T or A;
  • BrSNPA03064 is located at position 26316567 on chromosome A03, and its base is T or G;
  • BrSNPA03065 is located at position 26753682 on chromosome A03, and its base is G or A;
  • BrSNPA03066 is located at position 27196183 on chromosome A03, and its base is T or G;
  • BrSNPA03067 is located at position 27211545 on chromosome A03, and its base is C or A;
  • BrSNPA03068 is located at position 27471408 on chromosome A03, and its base is T or G;
  • BrSNPA03069 is located at position 28110115 on chromosome A03, and its base is G or C;
  • BrSNPA03070 is located at position 28193409 on chromosome A03, and its base is G or C;
  • BrSNPA03071 is located at position 28321741 on chromosome A03, and its base is G or A;
  • BrSNPA03072 is located at position 28629888 on chromosome A03, and its base is T or G;
  • BrSNPA03073 is located at position 28687095 on chromosome A03, and its base is G or A;
  • BrSNPA03074 is located at position 29060156 on chromosome A03, and its base is G or A;
  • BrSNPA03075 is located at position 29070757 on chromosome A03, and its base is C or A;
  • BrSNPA03076 is located at position 29068065 on chromosome A03, and its base is G or C;
  • BrSNPA03077 is located at position 30080620 on chromosome A03, and its base is T or C;
  • BrSNPA03078 is located at position 30648246 on chromosome A03, and its base is A or C;
  • BrSNPA04001 is located at position 556322 on chromosome A04, and its base is G or A;
  • BrSNPA04002 is located at position 898850 on chromosome A04, and its base is T or G;
  • BrSNPA04003 is located at position 1223515 on chromosome A04, and its base is G or A;
  • BrSNPA04004 is located at position 1378190 on chromosome A04, and its base is G or A;
  • BrSNPA04005 is located at position 1580901 on chromosome A04, and its base is T or C;
  • BrSNPA04006 is located at position 1661398 on chromosome A04, and its base is G or A;
  • BrSNPA04007 is located at position 1743434 on chromosome A04, and its base is G or A;
  • BrSNPA04008 is located at position 1833604 on chromosome A04, and its base is T or G;
  • BrSNPA04009 is located at position 2121284 on chromosome A04, and its base is G or A;
  • BrSNPA04010 is located at position 2232565 on chromosome A04, and its base is T or A;
  • BrSNPA04011 is located at position 2827196 on chromosome A04, and its base is G or A;
  • BrSNPA04012 is located at position 3162294 on chromosome A04, and its base is G or A;
  • BrSNPA04013 is located at position 3232728 on chromosome A04, and its base is G or C;
  • BrSNPA04014 is located at position 3760068 on chromosome A04, and its base is G or A;
  • BrSNPA04015 is located at position 3908277 on chromosome A04, and its base is G or A;
  • BrSNPA04016 is located at position 4437696 on chromosome A04, and its base is C or G;
  • BrSNPA04017 is located at position 4689758 on chromosome A04, and its base is T or C;
  • BrSNPA04018 is located at position 5060447 on chromosome A04, and its base is T or G;
  • BrSNPA04019 is located at position 6684805 on chromosome A04, and its base is G or C;
  • BrSNPA04020 is located at position 6933634 on chromosome A04, and its base is G or A;
  • BrSNPA04021 is located at position 8477239 on chromosome A04, and its base is A or C;
  • BrSNPA04022 is located at position 8662680 on chromosome A04, and its base is T or G;
  • BrSNPA04023 is located at position 9741929 on chromosome A04, and its base is G or T;
  • BrSNPA04024 is located at position 10563277 on chromosome A04, and its base is T or G;
  • BrSNPA04025 is located at position 11311194 on chromosome A04, and its base is G or C;
  • BrSNPA04026 is located at position 11 42806 on chromosome A04, and its base is G or A;
  • BrSNPA04027 is located at position 11984195 on chromosome A04, and its base is T or G;
  • BrSNPA04028 is located at position 12352714 on chromosome A04, and its base is T or A;
  • BrSNPA04029 is located at position 12579896 on chromosome A04, and its base is G or A;
  • BrSNPA04030 is located at position 13764622 on chromosome A04, and its base is A or G;
  • BrSNPA04031 is located at position 14413900 on chromosome A04, and its base is G or A;
  • BrSNPA04032 is located at position 15528641 on chromosome A04, and its base is G or A;
  • BrSNPA04033 is located at position 15560443 on chromosome A04, and its base is T or C;
  • BrSNPA04034 is located at position 15732203 on chromosome A04, and its base is G or C;
  • BrSNPA04035 is located at position 16065091 on chromosome A04, and its base is T or A;
  • BrSNPA04036 is located at position 16331059 on chromosome A04, and its base is G or C;
  • BrSNPA04037 is located at position 16851784 on chromosome A04, and its base is T or C;
  • BrSNPA04038 is located at position 17018800 on chromosome A04, and its base is G or C;
  • BrSNPA04039 is located at position 18819192 on chromosome A04, and its base is T or C;
  • BrSNPA04040 is located at position 19072439 on chromosome A04, and its base is T or C;
  • BrSNPA04041 is located at position 19212124 on chromosome A04, and its base is G or A;
  • BrSNPA05001 is located at position 487586 on chromosome A05, and its base is A or T;
  • BrSNPA05002 is located at position 1283352 on chromosome A05, and its base is C or T;
  • BrSNPA05003 is located at position 2024918 on chromosome A05, and its base is G or A;
  • BrSNPA05004 is located at position 2253083 on chromosome A05, and its base is T or G;
  • BrSNPA05005 is located at position 2688397 on chromosome A05, and its base is G or C;
  • BrSNPA05006 is located at position 2809229 on chromosome A05, and its base is C or A;
  • BrSNPA05007 is located at position 2835432 on chromosome A05, and its base is G or A;
  • BrSNPA05008 is located at position 2945096 on chromosome A05, and its base is C or A;
  • BrSNPA05009 is located at position 3063334 on chromosome A05, and its base is G or T;
  • BrSNPA05010 is located at position 3608353 on chromosome A05, and its base is T or A;
  • BrSNPA05011 is located at position 3663635 on chromosome A05, and its base is T or C;
  • BrSNPA05012 is located at position 3669632 on chromosome A05, and its base is G or A;
  • BrSNPA05013 is located at position 3702768 on chromosome A05, and its base is T or A;
  • BrSNPA05014 is located at position 3711749 on chromosome A05, and its base is T or C;
  • BrSNPA05015 is located at position 4073949 on chromosome A05, and its base is G or A;
  • BrSNPA05016 is located at position 4207921 on chromosome A05, and its base is G or C;
  • BrSNPA05017 is located at position 4232210 on chromosome A05, and its base is G or A;
  • BrSNPA05018 is located at position 4379811 on chromosome A05, and its base is T or A;
  • BrSNPA05019 is located at position 5944167 on chromosome A05, and its base is T or A;
  • BrSNPA05020 is located at position 6796365 on chromosome A05, and its base is T or G;
  • BrSNPA05021 is located at position 7357437 on chromosome A05, and its base is C or A;
  • BrSNPA05022 is located at position 7531884 on chromosome A05, and its base is G or A;
  • BrSNPA05023 is located at position 7551654 on chromosome A05, and its base is G or A;
  • BrSNPA05024 is located at position 8088873 on chromosome A05, and its base is C or T;
  • BrSNPA05025 is located at position 8959223 on chromosome A05, and its base is G or A;
  • BrSNPA05026 is located at position 9401373 on chromosome A05, and its base is T or C;
  • BrSNPA05027 is located at position 9714952 on chromosome A05, and its base is G or C;
  • BrSNPA05028 is located at position 9932276 on chromosome A05, and its base is T or C;
  • BrSNPA05029 is located at position 9890212 on chromosome A05, and its base is T or A;
  • BrSNPA05030 is located at position 10343678 on chromosome A05, and its base is G or A;
  • BrSNPA05031 is located at position 10705210 on chromosome A05, and its base is C or A;
  • BrSNPA05032 is located at position 10722668 on chromosome A05, and its base is T or C;
  • BrSNPA05033 is located at position 10887243 on chromosome A05, and its base is T or C;
  • BrSNPA05034 is located at position 14441564 on chromosome A05, and its base is G or A;
  • BrSNPA05035 is located at position 14794179 on chromosome A05, and its base is T or C;
  • BrSNPA05036 is located at position 14801567 on chromosome A05, and its base is T or G;
  • BrSNPA05037 is located at position 14960042 on chromosome A05, and its base is G or C;
  • BrSNPA05038 is located at position 16182627 on chromosome A05, and its base is T or A;
  • BrSNPA05039 is located at position 16282153 on chromosome A05, and its base is T or C;
  • BrSNPA05040 is located at position 16543992 on chromosome A05, and its base is T or C;
  • BrSNPA05041 is located at position 16988803 on chromosome A05, and its base is T or A;
  • BrSNPA05042 is located at position 17247727 on chromosome A05, and its base is T or A;
  • BrSNPA05043 is located at position 17810370 on chromosome A05, and its base is G or A;
  • BrSNPA05044 is located at position 18626934 on chromosome A05, and its base is T or G;
  • BrSNPA05045 is located at position 19905454 on chromosome A05, and its base is G or C;
  • BrSNPA05046 is located at position 19022188 on chromosome A05, and its base is T or C;
  • BrSNPA05047 is located at position 20978461 on chromosome A05, and its base is C or A;
  • BrSNPA05048 is located at position 21254133 on chromosome A05, and its base is C or A;
  • BrSNPA05049 is located at position 21498528 on chromosome A05, and its base is G or A;
  • BrSNPA05050 is located at position 22030048 on chromosome A05, and its base is C or T;
  • BrSNPA05051 is located at position 22731111 on chromosome A05, and its base is G or C;
  • BrSNPA05052 is located at position 22950070 on chromosome A05, and its base is C or A;
  • BrSNPA05053 is located at position 22962829 on chromosome A05, and its base is T or C;
  • BrSNPA06001 is located at position 18887 on chromosome A06, and its base is C or G;
  • BrSNPA06002 is located at position 117846 on chromosome A06, and its base is A or G;
  • BrSNPA06003 is located at position 1296897 on chromosome A06, and its base is T or C;
  • BrSNPA06004 is located at position 1752922 on chromosome A06, and its base is G or A;
  • BrSNPA06005 is located at position 2426330 on chromosome A06, and its base is G or A;
  • BrSNPA06006 is located at position 2789766 on chromosome A06, and its base is T or G;
  • BrSNPA06007 is located at position 2931644 on chromosome A06, and its base is T or C;
  • BrSNPA06008 is located at position 6382803 on chromosome A06, and its base is C or A;
  • BrSNPA06009 is located at position 6403261 on chromosome A06, and its base is C or A;
  • BrSNPA06010 is located at position 8245454 on chromosome A06, and its base is G or A;
  • BrSNPA06011 is located at position 8618748 on chromosome A06, and its base is A or G;
  • BrSNPA06012 is located at position 10107459 on chromosome A06, and its base is G or A;
  • BrSNPA06013 is located at position 10568157 on chromosome A06, and its base is G or A;
  • BrSNPA06014 is located at position 10588879 on chromosome A06, and its base is T or C;
  • BrSNPA06015 is located at position 10874817 on chromosome A06, and its base is G or C;
  • BrSNPA06016 is located at position 11069822 on chromosome A06, and its base is T or G;
  • BrSNPA06017 is located at position 11344725 on chromosome A06, and its base is T or A;
  • BrSNPA06018 is located at position 12784985 on chromosome A06, and its base is T or C;
  • BrSNPA06019 is located at position 13424538 on chromosome A06, and its base is C or A;
  • BrSNPA06020 is located at position 13447511 on chromosome A06, and its base is G or A;
  • BrSNPA06021 is located at position 13354897 on chromosome A06, and its base is A or T;
  • BrSNPA06022 is located at position 13545093 on chromosome A06, and its base is T or C;
  • BrSNPA06023 is located at position 14701558 on chromosome A06, and its base is T or C;
  • BrSNPA06024 is located at position 14726472 on chromosome A06, and its base is C or A;
  • BrSNPA06025 is located at position 14730894 on chromosome A06, and its base is T or C;
  • BrSNPA06026 is located at position 14731133 on chromosome A06, and its base is T or A;
  • BrSNPA06027 is located at position 15606985 on chromosome A06, and its base is G or A;
  • BrSNPA06028 is located at position 15685596 on chromosome A06, and its base is G or A;
  • BrSNPA06029 is located at position 16018969 on chromosome A06, and its base is C or A;
  • BrSNPA06030 is located at position 16193195 on chromosome A06, and its base is G or C;
  • BrSNPA06031 is located at position 16201857 on chromosome A06, and its base is G or A;
  • BrSNPA06032 is located at position 16271874 on chromosome A06, and its base is T or A;
  • BrSNPA06033 is located at position 17383582 on chromosome A06, and its base is G or A;
  • BrSNPA06034 is located at position 17610866 on chromosome A06, and its base is A or G;
  • BrSNPA06035 is located at position 17773836 on chromosome A06, and its base is T or A;
  • BrSNPA06036 is located at position 18376779 on chromosome A06, and its base is G or A;
  • BrSNPA06037 is located at position 18546656 on chromosome A06, and its base is G or C;
  • BrSNPA06038 is located at position 18584746 on chromosome A06, and its base is G or A;
  • BrSNPA06039 is located at position 18685199 on chromosome A06, and its base is T or G;
  • BrSNPA06040 is located at position 19361503 on chromosome A06, and its base is G or C;
  • BrSNPA06041 is located at position 19387887 on chromosome A06, and its base is G or A;
  • BrSNPA06042 is located at position 20048140 on chromosome A06, and its base is G or A;
  • BrSNPA06043 is located at position 20833714 on chromosome A06, and its base is G or C;
  • BrSNPA06044 is located at position 20977558 on chromosome A06, and its base is T or C;
  • BrSNPA06045 is located at position 21415891 on chromosome A06, and its base is G or A;
  • BrSNPA06046 is located at position 21632076 on chromosome A06, and its base is G or A;
  • BrSNPA06047 is located at position 22374291 on chromosome A06, and its base is C or G;
  • BrSNPA06048 is located at position 23153503 on chromosome A06, and its base is C or A;
  • BrSNPA06049 is located at position 23563378 on chromosome A06, and its base is G or A;
  • BrSNPA06050 is located at position 23928146 on chromosome A06, and its base is G or A;
  • BrSNPA06051 is located at position 23955274 on chromosome A06, and its base is T or A;
  • BrSNPA06052 is located at position 24041088 on chromosome A06, and its base is C or T;
  • BrSNPA06053 is located at position 24284057 on chromosome A06, and its base is G or A;
  • BrSNPA06054 is located at position 24719886 on chromosome A06, and its base is C or A;
  • BrSNPA06055 is located at position 25147238 on chromosome A06, and its base is T or C;
  • BrSNPA07001 is located at position 246275 on chromosome A07, and its base is G or C;
  • BrSNPA07002 is located at position 641403 on chromosome A07, and its base is G or A;
  • BrSNPA07003 is located at position 1028346 on chromosome A07, and its base is T or A;
  • BrSNPA07004 is located at position 2560851 on chromosome A07, and its base is G or A;
  • BrSNPA07005 is located at position 2970833 on chromosome A07, and its base is A or G;
  • BrSNPA07006 is located at position 3216036 on chromosome A07, and its base is T or C;
  • BrSNPA07007 is located at position 3279889 on chromosome A07, and its base is T or G;
  • BrSNPA07008 is located at position 3358700 on chromosome A07, and its base is C or A;
  • BrSNPA07009 is located at position 3961792 on chromosome A07, and its base is T or A;
  • BrSNPA07010 is located at position 4234366 on chromosome A07, and its base is T or C;
  • BrSNPA07011 is located at position 5021185 on chromosome A07, and its base is T or G;
  • BrSNPA07012 is located at position 5153068 on chromosome A07, and its base is T or G;
  • BrSNPA07013 is located at position 6101124 on chromosome A07, and its base is T or A;
  • BrSNPA07014 is located at position 6254138 on chromosome A07, and its base is T or A;
  • BrSNPA07015 is located at position 6429733 on chromosome A07, and its base is T or C;
  • BrSNPA07016 is located at position 7129127 on chromosome A07, and its base is T or C;
  • BrSNPA07017 is located at position 7137784 on chromosome A07, and its base is G or A;
  • BrSNPA07018 is located at position 7259222 on chromosome A07, and its base is C or A;
  • BrSNPA07019 is located at position 8773815 on chromosome A07, and its base is T or G;
  • BrSNPA07020 is located at position 0905585 on chromosome A07, and its base is G or A;
  • BrSNPA07021 is located at position 9463490 on chromosome A07, and its base is T or C;
  • BrSNPA07022 is located at position 9679954 on chromosome A07, and its base is T or C;
  • BrSNPA07023 is located at position 11143455 on chromosome A07, and its base is T or C;
  • BrSNPA07024 is located at position 11295388 on chromosome A07, and its base is G or A;
  • BrSNPA07025 is located at position 11498098 on chromosome A07, and its base is T or G;
  • BrSNPA07026 is located at position 11520532 on chromosome A07, and its base is G or A;
  • BrSNPA07027 is located at position 11551826 on chromosome A07, and its base is T or C;
  • BrSNPA07028 is located at position 11750525 on chromosome A07, and its base is G or A;
  • BrSNPA07029 is located at position 12234015 on chromosome A07, and its base is C or T;
  • BrSNPA07030 is located at position 13554367 on chromosome A07, and its base is G or A;
  • BrSNPA07031 is located at position 14074310 on chromosome A07, and its base is T or C;
  • BrSNPA07032 is located at position 14532031 on chromosome A07, and its base is T or C;
  • BrSNPA07033 is located at position 14747998 on chromosome A07, and its base is T or A;
  • BrSNPA07034 is located at position 15655059 on chromosome A07, and its base is A or G;
  • BrSNPA07035 is located at position 15740558 on chromosome A07, and its base is C or A;
  • BrSNPA07036 is located at position 16130782 on chromosome A07, and its base is T or C;
  • BrSNPA07037 is located at position 16491110 on chromosome A07, and its base is T or G;
  • BrSNPA07038 is located at position 17285904 on chromosome A07, and its base is G or A;
  • BrSNPA07039 is located at position 18060124 on chromosome A07, and its base is T or G;
  • BrSNPA07040 is located at position 18453973 on chromosome A07, and its base is C or T;
  • BrSNPA07041 is located at position 19178191 on chromosome A07, and its base is T or C;
  • BrSNPA07042 is located at position 19761996 on chromosome A07, and its base is G or A;
  • BrSNPA07043 is located at position 19,940,600 on chromosome A07, and its base is T or C;
  • BrSNPA07044 is located at position 20112319 on chromosome A07, and its base is G or C;
  • BrSNPA07045 is located at position 21087891 on chromosome A07, and its base is A or G;
  • BrSNPA07046 is located at position 21941825 on chromosome A07, and its base is T or C;
  • BrSNPA07047 is located at position 22261109 on chromosome A07, and its base is T or G;
  • BrSNPA07048 is located at position 22219912 on chromosome A07, and its base is T or C;
  • BrSNPA07049 is located at position 22522822 on chromosome A07, and its base is C or T;
  • BrSNPA07050 is located at position 23348479 on chromosome A07, and its base is G or A;
  • BrSNPA07051 is located at position 24460147 on chromosome A07, and its base is G or C;
  • BrSNPA07052 is located at position 25407338 on chromosome A07, and its base is T or A;
  • BrSNPA07053 is located at position 25732322 on chromosome A07, and its base is G or A;
  • BrSNPA07054 is located at position 25744414 on chromosome A07, and its base is A or C;
  • BrSNPA08001 is located at position 100717 on chromosome A08, and its base is C or G;
  • BrSNPA08002 is located at position 240722 on chromosome A08, and its base is G or A;
  • BrSNPA08003 is located at position 277090 on chromosome A08, and its base is T or C;
  • BrSNPA08004 is located at position 926709 on chromosome A08, and its base is G or A;
  • BrSNPA08005 is located at position 1715093 on chromosome A08, and its base is G or A;
  • BrSNPA08006 is located at position 2121093 on chromosome A08, and its base is G or A;
  • BrSNPA08007 is located at position 2374634 on chromosome A08, and its base is G or A;
  • BrSNPA08008 is located at position 3289888 on chromosome A08, and its base is T or G;
  • BrSNPA08009 is located at position 3424941 on chromosome A08, and its base is C or G;
  • BrSNPA08010 is located at position 3911814 on chromosome A08, and its base is T or G;
  • BrSNPA08011 is located at position 3915045 on chromosome A08, and its base is T or C;
  • BrSNPA08012 is located at position 4419556 on chromosome A08, and its base is C or T;
  • BrSNPA08013 is located at position 5430112 on chromosome A08, and its base is T or G;
  • BrSNPA08014 is located at position 6263634 on chromosome A08, and its base is C or A;
  • BrSNPA08015 is located at position 7150432 on chromosome A08, and its base is G or A;
  • BrSNPA08016 is located at position 7588851 on chromosome A08, and its base is T or C;
  • BrSNPA08017 is located at position 8359981 on chromosome A08, and its base is C or A;
  • BrSNPA08018 is located at chromosome 8933259 on chromosome A08, and its base is G or A;
  • BrSNPA08019 is located at position 9415744 on chromosome A08, and its base is T or G;
  • BrSNPA08020 is located at position 10869244 on chromosome A08, and its base is C or T;
  • BrSNPA08021 is located at position 11195840 on chromosome A08, and its base is T or A;
  • BrSNPA08022 is located at position 12382048 on chromosome A08, and its base is T or A;
  • BrSNPA08023 is located at position 12394480 on chromosome A08, and its base is T or A;
  • BrSNPA08024 is located at position 12855208 on chromosome A08, and its base is C or A;
  • BrSNPA08025 is located at position 12390981 on chromosome A08, and its base is G or C;
  • BrSNPA08026 is located at position 13838879 on chromosome A08, and its base is T or A;
  • BrSNPA08027 is located at position 14102664 on chromosome A08, and its base is G or C;
  • BrSNPA08028 is located at position 14112886 on chromosome A08, and its base is T or C;
  • BrSNPA08029 is located at position 14137581 on chromosome A08, and its base is T or C;
  • BrSNPA08030 is located at position 14580308 on chromosome A08, and its base is T or C;
  • BrSNPA08031 is located at position 14960288 on chromosome A08, and its base is G or C;
  • BrSNPA08032 is located at position 15507562 on chromosome A08, and its base is T or G;
  • BrSNPA08033 is located at position 15288418 on chromosome A08, and its base is G or C;
  • BrSNPA08034 is located at position 15734124 on chromosome A08, and its base is G or A;
  • BrSNPA08035 is located at position 16162051 on chromosome A08, and its base is T or G;
  • BrSNPA08036 is located at position 16415364 on chromosome A08, and its base is T or A;
  • BrSNPA08037 is located at position 16583368 on chromosome A08, and its base is T or C;
  • BrSNPA08038 is located at position 17179902 on chromosome A08, and its base is T or G;
  • BrSNPA08039 is located at position 17537125 on chromosome A08, and its base is T or C;
  • BrSNPA08040 is located at position 17877117 on chromosome A08, and its base is T or C;
  • BrSNPA08041 is located at position 18085207 on chromosome A08, and its base is T or C;
  • BrSNPA08042 is located at position 18668405 on chromosome A08, and its base is T or C;
  • BrSNPA08043 is located at position 19658257 on chromosome A08, and its base is G or A;
  • BrSNPA08044 is located at position 19860954 on chromosome A08, and its base is C or T;
  • BrSNPA08045 is located at position 19601288 on chromosome A08, and its base is C or T;
  • BrSNPA08046 is located at the number 20222413 on chromosome A08, and its base is G or C;
  • BrSNPA08047 is located at position 20416840 on chromosome A08, and its base is G or A;
  • BrSNPA08048 is located at position 20458612 on chromosome A08, and its base is T or C;
  • BrSNPA08049 is located at position 20716882 on chromosome A08, and its base is G or C;
  • BrSNPA09001 is located at position 165179 on chromosome A09, and its base is G or C;
  • BrSNPA09002 is located at position 211286 on chromosome A09, and its base is T or A;
  • BrSNPA09003 is located at position 443160 on chromosome A09, and its base is T or A;
  • BrSNPA09004 is located at position 583658 on chromosome A09, and its base is A or G;
  • BrSNPA09005 is located at position 898133 on chromosome A09, and its base is T or A;
  • BrSNPA09006 is located at position 1161182 on chromosome A09, and its base is T or C;
  • BrSNPA09007 is located at position 1379762 on chromosome A09, and its base is G or A;
  • BrSNPA09008 is located at position 1595348 on chromosome A09, and its base is T or G;
  • BrSNPA09009 is located at position 1627618 on chromosome A09, and its base is G or C;
  • BrSNPA09010 is located at position 1901646 on chromosome A09, and its base is T or A;
  • BrSNPA09011 is located at position 1930677 on chromosome A09, and its base is G or C;
  • BrSNPA09012 is located at position 3019969 on chromosome A09, and its base is G or T;
  • BrSNPA09013 is located at position 3817906 on chromosome A09, and its base is G or C;
  • BrSNPA09014 is located at position 3896403 on chromosome A09, and its base is G or A;
  • BrSNPA09015 is located at position 3903092 on chromosome A09, and its base is G or C;
  • BrSNPA09016 is located at position 4226515 on chromosome A09, and its base is G or A;
  • BrSNPA09017 is located at position 4354836 on chromosome A09, and its base is C or A;
  • BrSNPA09018 is located at position 4534869 on chromosome A09, and its base is C or A;
  • BrSNPA09019 is located at position 4566148 on chromosome A09, and its base is G or A;
  • BrSNPA09020 is located at position 4597771 on chromosome A09, and its base is G or C;
  • BrSNPA09021 is located at position 5122605 on chromosome A09, and its base is C or A;
  • BrSNPA09022 is located at position 5480210 on chromosome A09, and its base is T or C;
  • BrSNPA09023 is located at position 5575779 on chromosome A09, and its base is T or G;
  • BrSNPA09024 is located at position 6031641 on chromosome A09, and its base is T or C;
  • BrSNPA09025 is located at position 6984636 on chromosome A09, and its base is T or C;
  • BrSNPA09026 is located at position 7622,272 on chromosome A09, and its base is C or T;
  • BrSNPA09027 is located at position 7624286 on chromosome A09, and its base is G or T;
  • BrSNPA09028 is located at position 7897532 on chromosome A09, and its base is T or C;
  • BrSNPA09029 is located at position 8599991 on chromosome A09, and its base is G or T;
  • BrSNPA09030 is located at position 8973906 on chromosome A09, and its base is G or T;
  • BrSNPA09031 is located at position 8876252 on chromosome A09, and its base is T or A;
  • BrSNPA09032 is located at position 8877380 on chromosome A09, and its base is C or G;
  • BrSNPA09033 is located at position 9006311 on chromosome A09, and its base is C or A;
  • BrSNPA09034 is located at position 9062940 on chromosome A09, and its base is T or C;
  • BrSNPA09035 is located at position 9303625 on chromosome A09, and its base is T or G;
  • BrSNPA09036 is located at position 9805886 on chromosome A09, and its base is C or T;
  • BrSNPA09037 is located at position 10247545 on chromosome A09, and its base is C or A;
  • BrSNPA09038 is located at position 11056315 on chromosome A09, and its base is T or A;
  • BrSNPA09039 is located at position 11230572 on chromosome A09, and its base is G or A;
  • BrSNPA09040 is located at position 12895394 on chromosome A09, and its base is G or A;
  • BrSNPA09041 is located at position 13709942 on chromosome A09, and its base is T or C;
  • BrSNPA09042 is located at position 16256581 of chromosome A09, and its base is A or C;
  • BrSNPA09043 is located at position 17752348 on chromosome A09, and its base is G or C;
  • BrSNPA09044 is located at position 18332606 on chromosome A09, and its base is T or C;
  • BrSNPA09045 is located at position 18356344 on chromosome A09, and its base is G or A;
  • BrSNPA09046 is located at position 18406676 on chromosome A09, and its base is T or C;
  • BrSNPA09047 is located at position 18417691 on chromosome A09, and its base is T or G;
  • BrSNPA09048 is located at position 20994890 on chromosome A09, and its base is T or C;
  • BrSNPA09049 is located at position 21636788 on chromosome A09, and its base is G or A;
  • BrSNPA09050 is located at position 21654909 on chromosome A09, and its base is T or C;
  • BrSNPA09051 is located at position 21654935 on chromosome A09, and its base is T or C;
  • BrSNPA09052 is located at position 21910417 on chromosome A09, and its base is T or C;
  • BrSNPA09053 is located at position 22627850 on chromosome A09, and its base is G or A;
  • BrSNPA09054 is located at position 23220955 on chromosome A09, and its base is G or A;
  • BrSNPA09055 is located at position 23312031 on chromosome A09, and its base is G or A;
  • BrSNPA09056 is located at position 25785187 on chromosome A09, and its base is G or A;
  • BrSNPA09057 is located at position 26222141 on chromosome A09, and its base is T or C;
  • BrSNPA09058 is located at position 26503872 on chromosome A09, and its base is T or A;
  • BrSNPA09059 is located at position 26520788 on chromosome A09, and its base is G or C;
  • BrSNPA09060 is located at position 26564841 on chromosome A09, and its base is G or A;
  • BrSNPA09061 is located at position 26594548 on chromosome A09, and its base is T or A;
  • BrSNPA09062 is located at position 26739505 on chromosome A09, and its base is T or A;
  • BrSNPA09063 is located at position 26924828 on chromosome A09, and its base is G or C;
  • BrSNPA09064 is located at position 27270361 on chromosome A09, and its base is T or C;
  • BrSNPA09065 is located at position 28770187 on chromosome A09, and its base is T or C;
  • BrSNPA09066 is located at position 29198476 on chromosome A09, and its base is T or C;
  • BrSNPA09067 is located at position 30179678 on chromosome A09, and its base is G or A;
  • BrSNPA09068 is located at position 30448774 on chromosome A09, and its base is T or A;
  • BrSNPA09069 is located at position 30507943 on chromosome A09, and its base is T or C;
  • BrSNPA09070 is located at position 31873135 on chromosome A09, and its base is T or C;
  • BrSNPA09071 is located at position 31875876 on chromosome A09, and its base is T or C;
  • BrSNPA09072 is located at position 32719234 on chromosome A09, and its base is C or T;
  • BrSNPA09073 is located at position 32983605 on chromosome A09, and its base is G or A;
  • BrSNPA09074 is located at position 33491510 on chromosome A09, and its base is A or C;
  • BrSNPA09075 is located at position 34559009 on chromosome A09, and its base is G or A;
  • BrSNPA09076 is located at position 37047265 on chromosome A09, and its base is T or G;
  • BrSNPA09077 is located at position 37238842 on chromosome A09, and its base is C or A;
  • BrSNPA09078 is located at position 37695520 on chromosome A09, and its base is T or A;
  • BrSNPA09079 is located at position 37,741,739 on chromosome A09, and its base is G or A;
  • BrSNPA09080 is located at position 37743859 on chromosome A09, and its base is G or A;
  • BrSNPA10001 is located at position 9384 on chromosome A10, and its base is T or G;
  • BrSNPA10002 is located at position 119709 on chromosome A10, and its base is T or A;
  • BrSNPA10003 is located at position 617354 on chromosome A10, and its base is T or A;
  • BrSNPA10004 is located at position 1861569 on chromosome A10, and its base is T or C;
  • BrSNPA10005 is located at position 3689550 on chromosome A10, and its base is T or C;
  • BrSNPA10006 is located at position 3806065 on chromosome A10, and its base is G or A;
  • BrSNPA10007 is located at position 3721991 on chromosome A10, and its base is T or C;
  • BrSNPA10008 is located at position 3800101 on chromosome A10, and its base is G or A;
  • BrSNPA10009 is located at position 3834274 on chromosome A10, and its base is C or A;
  • BrSNPA10010 is located at position 3849310 on chromosome A10, and its base is C or A;
  • BrSNPA10011 is located at position 3870334 on chromosome A10, and its base is T or A;
  • BrSNPA10012 is located at position 4497856 on chromosome A10, and its base is T or A;
  • BrSNPA10013 is located at position 4782602 on chromosome A10, and its base is T or C;
  • BrSNPA10014 is located at position 5601251 on chromosome A10, and its base is G or C;
  • BrSNPA10015 is located at position 5671082 on chromosome A10, and its base is G or A;
  • BrSNPA10016 is located at position 6062425 on chromosome A10, and its base is G or A;
  • BrSNPA10017 is located at position 6294669 on chromosome A10, and its base is T or G;
  • BrSNPA10018 is located at position 6363410 on chromosome A10, and its base is T or G;
  • BrSNPA10019 is located at position 6787381 on chromosome A10, and its base is C or A;
  • BrSNPA10020 is located at position 7174178 on chromosome A10, and its base is G or C;
  • BrSNPA10021 is located at position 7999917 on chromosome A10, and its base is G or C;
  • BrSNPA10022 is located at position 8425118 on chromosome A10, and its base is T or C;
  • BrSNPA10023 is located at position 8648405 on chromosome A10, and its base is T or A;
  • BrSNPA10024 is located at position 9700540 on chromosome A10, and its base is G or C;
  • BrSNPA10025 is located at position 10373824 on chromosome A10, and its base is G or A;
  • BrSNPA10026 is located at position 10740098 on chromosome A10, and its base is T or C;
  • BrSNPA10027 is located at position 12134922 on chromosome A10, and its base is A or G;
  • BrSNPA10028 is located at position 12492205 on chromosome A10, and its base is G or C;
  • BrSNPA10029 is located at position 12527035 on chromosome A10, and its base is G or C;
  • BrSNPA10030 is located at position 12619426 on chromosome A10, and its base is G or A;
  • BrSNPA10031 is located at position 13105346 on chromosome A10, and its base is C or A;
  • BrSNPA10032 is located at position 13127812 on chromosome A10, and its base is G or T;
  • BrSNPA10033 is located at position 13406296 on chromosome A10, and its base is T or G;
  • BrSNPA10034 is located at position 13716056 on chromosome A10, and its base is T or A;
  • BrSNPA10035 is located at position 13732849 on chromosome A10, and its base is T or C;
  • BrSNPA10036 is located at position 13859895 on chromosome A10, and its base is G or A;
  • BrSNPA10037 is located at position 14319070 on chromosome A10, and its base is T or A;
  • BrSNPA10038 is located at position 14443918 on chromosome A10, and its base is C or A;
  • BrSNPA10039 is located at position 14456630 on chromosome A10, and its base is G or C;
  • BrSNPA10040 is located at position 14638538 on chromosome A10, and its base is C or A;
  • BrSNPA10041 is located at position 15240989 on chromosome A10, and its base is G or C;
  • BrSNPA10042 is located at position 15835648 on chromosome A10, and its base is G or C;
  • BrSNPA10043 is located at position 16198723 on chromosome A10 and has a base of G or A.
  • Example 2 using the 571 SNP sites screened in Example 1 to design a KASP primer combination for detecting the corresponding SNP locus genotype and establishing a genotype detection method
  • the 571 SNP sites screened according to Example 1 were designed to detect the corresponding SNP locus genotype KASP primer combinations.
  • a set of 571 sets of KASP primers for the 571 SNP sites were designed, and each set of KASP primers was used to detect one of the 571 SNP sites.
  • Each set of KASP primers has two upstream primers (denoted as upstream primer 1 and upstream primer 2) and one downstream primer.
  • the structural composition of the upstream primer 1 is sequentially from the 5' end to the 3' end: a universal sequence 1 + a genome-specific sequence + one of two nucleotides at the SNP site;
  • the structural composition of the upstream primer 2 is sequentially from the 5' end to the 3' end: a universal sequence 2+ genome-specific sequence + the other of the two nucleotides at the SNP site;
  • the downstream primer consists only of genomic specific sequences.
  • the 3' end of the upstream primer is an allelic variation base.
  • the universal sequence 1 used in the present invention is a FAM fluorescent tag sequence 5'-GAAGGTGACCAAGTTCATGCT-3'
  • the universal sequence 2 is a HEX fluorescent tag sequence 5'-GAAGGTCGGAGTCAACGGATT-3'.
  • the KASP primer combination designed by the present invention for the 571 SNP sites screened in Example 1 consisted of 1713 single-stranded DNAs shown by SEQ ID NO: 1713 in the Sequence Listing.
  • sequences 1, 4, 7, ..., 1171 are upstream primers 1 for the 571 SNP sites, from 5'
  • the first 21 nucleotides are the universal sequence 1 (FAM fluorescent tag sequence), the subsequent sequence is a genome-specific sequence, and the 3' end is one of the two nucleotides corresponding to the SNP site; , 5, 8, ..., 1172 (ie, the sequence 3N-1, wherein N is an integer greater than or equal to 1 and less than or equal to 571) is the upstream primer 2 for the 571 SNP sites, starting from the 5' end 21
  • the nucleotide is the universal sequence 2 (HEX fluorescent tag sequence), the subsequent sequence is a genome-specific sequence, and the 3' end is the other of the two nucleotides corresponding to the SNP site; the sequence 3, 6, 9, ..., 1173 (i.e., sequence 3N, wherein N is an integer
  • the three primers shown in 1-3 were used to detect the SNP site BrSNPA01001 (the first of the 571 SNP sites in Example 1); the three primers shown in SEQ ID NO: 4-6 were used to detect the SNP site.
  • BrSNPA01002 (the second of the 571 SNP sites in Example 1); the three primers shown in SEQ ID NO: 7-9 were used to detect the SNP site BrSNPA01003 (the third of the 571 SNP sites in Example 1)
  • the three primers shown in the sequences 1711-1713 were used to detect the SNP site BrSNPA10043 (the 571th of the 571 SNP sites in Example 1).
  • the genomic DNA of the tested cabbage material was extracted by the conventional CTAB method.
  • the quality of the extracted DNA was detected by agarose electrophoresis and Nanodrop 2100, respectively. It was found that the extracted genomic DNA met the relevant quality requirements, that is, agarose electrophoresis showed that the DNA bands were single and there was no obvious dispersion; Nanodrop 2100 detected A260/280 between 1.8- Between 2.0 (DNA samples are free of protein contamination); A260/230 is between 1.8 and 2.0 (lower salt ion concentration in DNA samples); no significant light absorption at 270 nm (no phenol contamination in DNA samples); for competitive alleles
  • the amount of DNA detected by the gene-specific PCR technique was 4-10 ng per sample. The diluted DNA concentration became 4-10 ng/ ⁇ l for use.
  • each KASP primer in the KASP primer combination developed in Example 1 was separately subjected to PCR amplification to obtain a PCR amplification product.
  • the KASPar reaction was carried out in a 384-well plate (Part No. KBS-0750-001) or a 1536 microplate (Part No. KBS-0751-001) with a reaction system of 3 ⁇ l or 1 ⁇ l.
  • the K-pette liquid separation workstation is used to add the DNA template to be tested in the microplate. (4 ⁇ 10 ng / ⁇ l) 1.5 ⁇ l, dried at 60 ° C. Then, using a Meridian loading station under the Kraken operating system, a Master Mix with a final concentration of 1 ⁇ (diluted from Part No. KBS-1016-002 or Part No. KBS-1016-011) was added to each well. Primer premix (the two upstream primers and the downstream primer are mixed at a molar concentration ratio of 6:6:15, and the final concentration of each primer is 10 ⁇ M). Immediately after the Mix is dispensed, the microplates are placed in turn on the Kube heat sealer and the Fusion laser seal. The membrane is sealed on the membrane.
  • the PCR reaction was carried out in a high-flux water bath system Hydrocycler.
  • the specific procedure was: pre-denaturation at 94 °C for 15 minutes; denaturation at 94 °C for 20 seconds, renaturation & extension at 61 °C-55 °C for 1 minute, and amplification for 10 cycles with the touch down program.
  • the renaturation & extension temperature was reduced by 0.6 ° C per cycle; denaturation at 94 ° C for 20 seconds, 55 ° C for 60 seconds, 26 cycles.
  • the experiment also set a blank control in the reaction system without adding template DNA. At least 3 replicates were set for each PCR amplification product sample.
  • the BMG PHERAstar instrument was used to detect the fluorescence signal and to view the typing. If the classification is not sufficient, continue to expand, and check the classification every 3 cycles until the classification is complete.
  • Example 3 using the KASP primer combination designed in Example 2 to detect 571 SNP loci genotypes to construct a cabbage DNA fingerprint database
  • the genomic DNA of each of the tested cabbage varieties obtained in the step 1 was subjected to the detection of the 571 SNP loci in Example 1.
  • the specific operation method refer to step 2 of Embodiment 2.
  • the genotypic data of the 571 SNP loci of each of the tested cabbage varieties finally formed the cabbage DNA fingerprint database.
  • Example 4 Analysis and evolution analysis of genetic diversity of Chinese cabbage using SNP cabbage DNA fingerprint database obtained in Example 3
  • the 571 SNP loci provided by the present invention can be used for the analysis of cabbage genetic resources and taxonomic division since it has been considered to distinguish subspecies and varieties of Chinese cabbage.
  • the genotypic data of 571 SNP sites obtained using Examples 1-3 are detailed below for grouping of representative inbred line samples of 255 different Chinese cabbage subspecies and varieties (same as Example 3).
  • Narinosa (Nar), ssp.chinensis var.parachinensis (P), moss Ssp.chinensis var.tsai-cai(T), ssp.chinensis var.multiceps(J), and ssp.chinensis var.nai-bai(N), ssp.chinensis var.hei-ye(H)
  • oil cabbage Brassica rapa ssp.oleifera (O) and Brassica rapa ssp.rapifera (R) can be completely distinguished.
  • the inbred line material (donor) with good traits and the recurrent parent (receptor) with better comprehensive traits are usually backcrossed for many generations, so that the overall trait is similar to that of the recurrent parent. , but increased the progeny of the favorable traits transferred from the donor.
  • the 571 SNP loci can be used to select the intermediate materials in the backcross breeding process to achieve the goal of quickly obtaining a new line of target traits.
  • the Journal of Agricultural Sciences (1989) has a good comprehensive agronomic trait, but it is not resistant to dry heart disease;
  • the parent material, Zhenlv No.6 variety "Zhenlu 6", obtained the inbred line after six generations of self-delivery, Zhenlv No.6 Available from Tianjin Kerun Agricultural Technology Co., Ltd.
  • Comprehensive agronomic traits are common, but dry heart disease resistance is outstanding.
  • the inventors of the present invention firstly hybridize the two-leaf (receptor) and the rare green No. 6 (donor), and then reincarnate with the two leaves. Parents are selected for breeding.
  • F1BC3 dry heart-resistant materials 571 SNP sites were used for screening. The results showed that the T1 and T20 materials of F1BC3 were the most similar to the two leaves, and the similarities were 99% and 97.6%, respectively. (Fig. 3), the agronomic traits observed in the later period showed that T224 and T20 did have better agronomic traits such as the second leaf. .

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Abstract

本发明公开了一种用于白菜遗传多样性分析及分子育种的SNP组合及其应用。本发明所提供的应用具体为571个SNP位点或用于检测所述571个SNP位点的物质在如下任一中的应用：(1)构建白菜DNA指纹数据库；(2)白菜种质资源遗传多样性分析和/或进化分析；(3)白菜分子育种；所述571个SNP位点的物理位置是基于白菜品种Chiifu-402-2的全基因组序列比对确定的，所述白菜品种Chiifu-402-2的全基因组序列的版本号为V1.5；所述571个SNP位点是BrSNPA01001-BrSNPA10043。实验证明，这571个SNP位点能够应用于白菜遗传多样性分析、进化分析；同时这571个SNP位点还可用于白菜分子育种，为其提供重要的技术支撑。

Description

用于白菜种质资源多样性分析及分子育种的SNP组合及其应用 技术领域
本发明属于分子生物学领域，涉及一种用于白菜种质资源多样性分析及分子育种的SNP组合及其应用。
背景技术
我国是世界蔬菜生产和消费第一大国，而白菜(Brassica rapa L.ssp.Pekinensis)是我国栽培面积最大的蔬菜作物，在菜篮子工程中的作用举足轻重。同时，由于白菜为杂交作物，其重组率高、遗传多样性丰富、以及较好的基因组学研究基础而成为遗传学研究的模式植物。我国是世界上白菜类作物种质资源最丰富的国家，除了常见的普通结球和不结球白菜外，还有京水菜、苔菜、菜薹、芜菁和油用白菜等多种类型，广泛分布于我国各个地区。这些类型与普通结球/不结球白菜相比，在形态上有明显的差异，但其食用和商品价值却非常高，是重要的育种材料。
实践表明，多种多样的种质资源是作物育种的物质基础。然而在现代白菜育种取得显著成就的同时，其种质资源有遗传基础日益贫乏的趋势，原因有：1.人们总是按照一定目标、沿着一定方向进行选择，选择时间越长、强度越大，品种的遗传基础就越窄。2.杂交育种中使用的亲本，越来越集中到综合性状最好、配合力最佳的少数几个材料上，这样就导致众多品种之间的亲缘相近。3.新品种的不断育成和推广，使原有老品种特别是地方品种逐渐被淘汰，但却未作为种质保存下来，致使许多有益的基因随之丢失。由于以上原因，使得对现有种质资源的保存、评价和分类的重要性愈益突出。预期在未来农业中，作物种质资源的丰富程度和有关研究工作的深入程度将决定作物育种的优势。
杂交种是目前白菜类作物的主导育种方式，正确选择亲本并予以合理组配是育种成败的关键。此外，通过杂交、回交导入外源遗传物质，是扩大遗传变异的重要途径。但上述过程都有一个共同问题亟待解决：无论是杂交还是回交，双亲或供体亲本中既有对作物改良有利的基因，也存在着大量的对作物改良不利的基因，这就需要一种准确有效的识别方法对获得的新种质进行背景选择，以确保尽可能地获取那些带有外源有益基因而整体背景又和目标亲本类似的新种质。
以前对白菜种质资源的评价和分类主要依赖于形态特征和RFLP、RAPD、AFLP和SSR等分子标记技术，这些技术的相互结合促进了我国对白菜类种质资源的评价与利用。但这些传统分子标记密度低，尤其不能实现对样品的高通量检测，大大限制了其在资源多样性分析方面的应用。实践证明SNP分子标记是检测种质资源遗传多样性和进行背景选择的有效工具，与其他类型分子标记相比，其具有数量多、全基因组覆盖、可实现高通量检测和操作周期短等显著优势。此外，其遗传稳定性要比SSR等遗传标记高得多，遗传分析或基因诊断时的重现性、准确性都优于其它标记。研究表明，SNP标记已经大规模应用于种质资源遗传多样性的评价及分类、种质筛选、品种认证和分子育种等多个领域。基于KASP(竞争性等位基因特异性 PCR)的SNPline基因分型检测是英国LGC(Laboratory of the Government Chemist)有限公司开发的高通量SNP分型技术，其具有准确、灵活、低成本、高通量的特点，目前已经成为国际上SNP分析的主流方法之一。
发明公开
本发明的目的是提供一种用于白菜遗传多样性分析及分子育种的SNP组合、引物组合及其在育种中的应用。
本发明所提供的应用具体为571个SNP位点或用于检测所述571个SNP位点的物质在如下任一中的应用：
(1)白菜种质资源遗传多样性分析和/或进化分析；
(2)白菜分子育种；
(3)构建白菜DNA指纹数据库；
(4)对白菜进行基因分型；
(5)检测任两个白菜品种的相似性。
所述571个SNP位点的物理位置是基于白菜品种Chiifu-402-2的全基因组序列比对确定的，所述白菜品种Chiifu-402-2的全基因组序列的版本号为V1.5(下载地址http://brassicadb.org/brad)；所述571个SNP位点是BrSNPA01001-BrSNPA10043(具体见实施例1)。
其中，所述“用于检测所述571个SNP位点的物质”可为任何能够检测所述571个SNP位点的基因型的试剂、仪器、设备等。在本发明中，所述“用于检测所述571个SNP位点的物质”具体为用于检测所述571个SNP位点的571组引物，每组引物用于检测所述571个SNP位点中的一种，所述571组引物中的每组引物由分别记为上游引物1和上游引物2的两条上游引物和一条下游引物组成；所述上游引物1的结构组成自5’端至3’端依次为：通用序列1、基因组特异序列、所述SNP位点处两种核苷酸中的一种；所述上游引物2的结构组成自5’端至3’端依次为：通用序列2、基因组特异序列、所述SNP位点处两种核苷酸中的另一种；所述下游引物仅由基因组特异序列组成。
进一步，在所述571组引物中，所有的所述上游引物中的基因组特异序列为将序列表中所有的序列X去除第1-21位后所得的1142个序列；所有的所述下游引物的核苷酸序列为序列表中所有的序列Y；其中，X为3N-2或3N-1，Y为3N，N为大于等于1且小于等于571的整数。即：序列表中共1142个序列n(n为1-1713中不是3的倍数的正整数，如1、2、4、5、7、8、……1711、1712)均去除第1-21位后所得的1142个序列为所有所述上游引物中的基因组特异序列；序列表中共571个序列m(m为1-1713中为3的倍数的正整数，如3、6、9、……、1713)为所有所述下游引物。
更进一步，所述通用序列1的核苷酸序列如序列表中序列1的第1-21所示；所述通用序列2的核苷酸序列如序列表中序列2的第1-22所示。
更加具体的，在本发明中，所述571组引物由序列表中序列1-序列1713所示 的1713条单链DNA组成。
其中，序列1-3所示的3个引物用于检测SNP位点BrSNPA01001(实施例1中571个SNP位点中的第1个)；序列4-6所示的3个引物用于检测SNP位点BrSNPA01002(实施例1中571个SNP位点中的第2个)；序列7-9所示的3个引物用于检测SNP位点BrSNPA01003(实施例1中571个SNP位点中的第3个)；……，以此类推，……，序列1711-1713所示的3个引物用于检测SNP位点BrSNPA10043(实施例1中571个SNP位点中的第571个)。
对于如上所述(3)的应用而言，在白菜新品种选育过程中，通常将具有优良性状的自交系材料(供体)和综合性状较好的轮回亲本(受体)进行多代的回交，从而获得整体性状与轮回亲本相似，但增加了从供体转来的有利性状的后代。利用所述571个SNP位点可对回交育种过程中的中间材料进行背景选择，以达到快速获得整合了目标性状新品系的目的。
本发明还提供了一种构建白菜DNA指纹数据库的方法。
本发明所提供的构建白菜DNA指纹数据库的方法，具体可包括如下步骤：
(a1)分别提取用于构建白菜DNA指纹数据库的所有白菜品种的基因组DNA；
(a2)检测所述所有白菜品种的基因组DNA中所述571个SNP位点的基因型，所得基因型数据即构成白菜DNA指纹数据库。
在所述方法的步骤(a2)中，具体是采用如上所述的“用于检测所述571个SNP位点的物质”检测所述所有白菜品种的基因组DNA中所述571个SNP位点的基因型的。
本发明还提供了一种对白菜种质资源进行类群划分的方法。
本发明所提供的对白菜种质资源进行类群划分的方法，具体可包括如下步骤：
(b1)分别提取待进行类群划分的所有白菜品种的基因组DNA；
(b2)检测所有所述白菜品种的基因组DNA中所述571个SNP位点的基因型，根据所得基因型数据，建立聚类树状图，从而将所述所有白菜品种划分为不同类群。
在所述方法的步骤(b2)中，具体是采用如上所述的“用于检测所述571个SNP位点的物质”检测所述所有白菜品种的基因组DNA中所述571个SNP位点的基因型的。
在步骤(b2)中，所述“根据所得基因型数据，建立聚类树状图(如UPGMA法聚类树状图)”具体为：根据所得基因型数据，利用Power-Marker ver.3.25软件，选用Rogers 1972遗传距离计算法，分析建立UPGMA法聚类树状图。
在实际应用中，可以根据利用本发明所提供的方法构建的白菜DNA指纹数据库和/或建立的白菜种质资源的聚类树状图确定待测白菜品种是否是新的种质材料。
本发明还提供了一种白菜分子育种方法。
本发明所提供的白菜分子育种方法，具体可包括如下步骤：将携带有目标性状的白菜供体亲本与白菜受体亲本(具有较好的综合性状)杂交，再将所得杂交后代 与所述白菜供体亲本进行若干次回交，分别检测所述白菜受体亲本以及所得的携带有所述目标性状的回交后代基因组DNA中所述571个SNP位点的基因型，根据检测结果从携带有所述目标性状的所述回交后代中选择所述571个SNP位点的基因型与所述白菜受体亲本一致性最高的个体。
本发明还提供了一种用于检测所述571个SNP位点的基因型的产品。
本发明所提供的用于检测所述571个SNP位点的基因型的产品，含有用于检测所述571个SNP位点的如上所述的571组引物。
所述产品在检测或辅助检测白菜基因组DNA中所述571个SNP位点的基因型中的应用也属于本发明的保护范围。
在本发明中，所述白菜可为结球白菜Brassica rapa ssp.Pekinensis、不结球白菜Brassica rapa ssp.Chinensis、油用白菜Brassica rapa ssp.oleifera(O)和芜菁Brassica rapa ssp.rapifera(R)；具体可选自如下表1所示品种中的全部或部分。
附图说明
图1为571个SNP位点在检测的255份白菜材料(表1)中有良好的多态性。其中，A为平均多态信息含量(PIC)；B为最小等位基因频率MAF；C为杂合度(Ho)；D为平均遗传多样性(GeneDiversity)。
图2为基于实施例3获得的255份白菜样品(表1)571个SNP位点的基因型数据对255份白菜样品进行类群划分。
图3为255份白菜材料(表1)的群体结构分析。其中，A为利用GGT2.0软件对测试样品进行基因分型；B为利用Power Marker和MEGA6.0对该测试样品进行聚类；C为相关测试样品照片。图中，303#表示“珍绿6号”；32#表示“胶二叶”。
实施发明的最佳方式
以下的实施例便于更好地理解本发明，但并不限定本发明。下述实施例中的实验方法，如无特殊说明，均为常规方法。下述实施例中所用的试验材料，如无特殊说明，均为自常规生化试剂商店购买得到的。
下述实施例中涉及的255份白菜种质样品如表1所示。
表1 255份白菜种质样品信息
Figure PCTCN2015095671-appb-000001
Figure PCTCN2015095671-appb-000002
Figure PCTCN2015095671-appb-000003
Figure PCTCN2015095671-appb-000004
Figure PCTCN2015095671-appb-000005
Figure PCTCN2015095671-appb-000006
Figure PCTCN2015095671-appb-000007
Figure PCTCN2015095671-appb-000008
注：表中绝大多数白菜为市面常售品种。
实施例1、用于白菜遗传多样性分析、进化分析及分子育种的571个SNP位点的获得
基于遗传背景具有广泛代表性的255份白菜种质样品(表1)重测序数据，对每个位点根据以下特征进行筛选评估：(a)，基因组均匀分布；(b)，SNP等位位点纯合程度>95％；(c)，最小等位基因频率在0.1-0.5之间；(d)，重测序深度>5×。最终得到用于白菜DNA指纹数据库构建、品种真实性检测、遗传资源分析及分子育种的571个SNP位点。
检测结果显示，选择的571个SNP位点在检测的255份白菜材料(表1)中有良好的多态性：(1)平均多态信息含量(PIC)为0.35；(2)MAF>0.1的比例为99.1％；(3)杂合度>0.1的比例为10％；(4)平均遗传多样性为0.35。详见图1。
这571个SNP位点的物理位置是基于白菜高代自交系Chiifu-401-42的全基因 组序列比对确定的；
所述白菜品种Chiifu-401-42全基因组序列的版本号为V1.5；
所述571个SNP位点具体如下BrSNPA01001-BrSNPA10043：
BrSNPA01001位于A01号染色体上第612144位，其碱基为T或A；
BrSNPA01002位于A01号染色体上第1059908位，其碱基为T或C；
BrSNPA01003位于A01号染色体上第1248141位，其碱基为T或C；
BrSNPA01004位于A01号染色体上第1385646位，其碱基为T或A；
BrSNPA01005位于A01号染色体上第1795429位，其碱基为T或C；
BrSNPA01006位于A01号染色体上第2112068位，其碱基为A或T；
BrSNPA01007位于A01号染色体上第2437505位，其碱基为G或A；
BrSNPA01008位于A01号染色体上第2830301位，其碱基为G或A；
BrSNPA01009位于A01号染色体上第2969334位，其碱基为G或A；
BrSNPA01010位于A01号染色体上第2970196位，其碱基为T或C；
BrSNPA01011位于A01号染色体上第3520140位，其碱基为T或C；
BrSNPA01012位于A01号染色体上第4182701位，其碱基为T或C；
BrSNPA01013位于A01号染色体上第4219165位，其碱基为C或A；
BrSNPA01014位于A01号染色体上第4225329位，其碱基为T或G；
BrSNPA01015位于A01号染色体上第4878177位，其碱基为T或C；
BrSNPA01016位于A01号染色体上第5849627位，其碱基为T或G；
BrSNPA01017位于A01号染色体上第6000315位，其碱基为C或G；
BrSNPA01018位于A01号染色体上第6385755位，其碱基为T或C；
BrSNPA01019位于A01号染色体上第7102966位，其碱基为T或A；
BrSNPA01020位于A01号染色体上第7105232位，其碱基为G或A；
BrSNPA01021位于A01号染色体上第7567812位，其碱基为T或C；
BrSNPA01022位于A01号染色体上第8475689位，其碱基为C或T；
BrSNPA01023位于A01号染色体上第9716212位，其碱基为G或C；
BrSNPA01024位于A01号染色体上第9865712位，其碱基为T或A；
BrSNPA01025位于A01号染色体上第10433882位，其碱基为A或G；
BrSNPA01026位于A01号染色体上第10454017位，其碱基为T或C；
BrSNPA01027位于A01号染色体上第11039761位，其碱基为T或C；
BrSNPA01028位于A01号染色体上第11576989位，其碱基为G或A；
BrSNPA01029位于A01号染色体上第12681321位，其碱基为T或G；
BrSNPA01030位于A01号染色体上第12708200位，其碱基为T或C；
BrSNPA01031位于A01号染色体上第12834470位，其碱基为G或C；
BrSNPA01032位于A01号染色体上第13128980位，其碱基为T或C；
BrSNPA01033位于A01号染色体上第14048162位，其碱基为T或C；
BrSNPA01034位于A01号染色体上第14897192位，其碱基为C或T；
BrSNPA01035位于A01号染色体上第15956879位，其碱基为T或C；
BrSNPA01036位于A01号染色体上第16592150位，其碱基为G或C；
BrSNPA01037位于A01号染色体上第17311260位，其碱基为T或C；
BrSNPA01038位于A01号染色体上第18199499位，其碱基为T或C；
BrSNPA01039位于A01号染色体上第18291203位，其碱基为T或C；
BrSNPA01040位于A01号染色体上第18635734位，其碱基为G或A；
BrSNPA01041位于A01号染色体上第18650317位，其碱基为G或A；
BrSNPA01042位于A01号染色体上第18657425位，其碱基为T或C；
BrSNPA01043位于A01号染色体上第18964955位，其碱基为G或C；
BrSNPA01044位于A01号染色体上第20057539位，其碱基为G或A；
BrSNPA01045位于A01号染色体上第21096109位，其碱基为C或A；
BrSNPA01046位于A01号染色体上第21986324位，其碱基为G或A；
BrSNPA01047位于A01号染色体上第22365926位，其碱基为G或C；
BrSNPA01048位于A01号染色体上第22417219位，其碱基为G或A；
BrSNPA01049位于A01号染色体上第22449302位，其碱基为G或A；
BrSNPA01050位于A01号染色体上第22523311位，其碱基为C或T；
BrSNPA01051位于A01号染色体上第22600539位，其碱基为T或C；
BrSNPA01052位于A01号染色体上第22695943位，其碱基为C或A；
BrSNPA01053位于A01号染色体上第24361302位，其碱基为T或C；
BrSNPA01054位于A01号染色体上第24456539位，其碱基为G或T；
BrSNPA01055位于A01号染色体上第24937773位，其碱基为G或A；
BrSNPA01056位于A01号染色体上第25108874位，其碱基为T或C；
BrSNPA01057位于A01号染色体上第26035077位，其碱基为T或A；
BrSNPA01058位于A01号染色体上第26549708位，其碱基为C或T；
BrSNPA01059位于A01号染色体上第26752589位，其碱基为G或A；
BrSNPA02001位于A02号染色体上第356794位，其碱基为C或T；
BrSNPA02002位于A02号染色体上第751652位，其碱基为G或A；
BrSNPA02003位于A02号染色体上第884677位，其碱基为T或A；
BrSNPA02004位于A02号染色体上第1026373位，其碱基为T或C；
BrSNPA02005位于A02号染色体上第1065889位，其碱基为T或A；
BrSNPA02006位于A02号染色体上第1142477位，其碱基为T或C；
BrSNPA02007位于A02号染色体上第2421548位，其碱基为A或C；
BrSNPA02008位于A02号染色体上第2663267位，其碱基为T或C；
BrSNPA02009位于A02号染色体上第2865799位，其碱基为T或C；
BrSNPA02010位于A02号染色体上第3245711位，其碱基为C或A；
BrSNPA02011位于A02号染色体上第3685320位，其碱基为G或A；
BrSNPA02012位于A02号染色体上第3812885位，其碱基为T或G；
BrSNPA02013位于A02号染色体上第4523362位，其碱基为T或A；
BrSNPA02014位于A02号染色体上第5184035位，其碱基为T或C；
BrSNPA02015位于A02号染色体上第6035955位，其碱基为T或G；
BrSNPA02016位于A02号染色体上第6431221位，其碱基为A或C；
BrSNPA02017位于A02号染色体上第6709555位，其碱基为C或A；
BrSNPA02018位于A02号染色体上第6957629位，其碱基为G或A；
BrSNPA02019位于A02号染色体上第8447338位，其碱基为T或C；
BrSNPA02020位于A02号染色体上第9141887位，其碱基为G或C；
BrSNPA02021位于A02号染色体上第9423555位，其碱基为T或C；
BrSNPA02022位于A02号染色体上第9490758位，其碱基为G或A；
BrSNPA02023位于A02号染色体上第11160802位，其碱基为C或A；
BrSNPA02024位于A02号染色体上第11181828位，其碱基为T或A；
BrSNPA02025位于A02号染色体上第11698932位，其碱基为T或A；
BrSNPA02026位于A02号染色体上第11780457位，其碱基为T或A；
BrSNPA02027位于A02号染色体上第13059562位，其碱基为G或A；
BrSNPA02028位于A02号染色体上第13619247位，其碱基为G或C；
BrSNPA02029位于A02号染色体上第14369430位，其碱基为T或A；
BrSNPA02030位于A02号染色体上第14606869位，其碱基为T或C；
BrSNPA02031位于A02号染色体上第14862673位，其碱基为T或C；
BrSNPA02032位于A02号染色体上第15736514位，其碱基为G或A；
BrSNPA02033位于A02号染色体上第16465013位，其碱基为G或C；
BrSNPA02034位于A02号染色体上第17440083位，其碱基为T或C；
BrSNPA02035位于A02号染色体上第17778839位，其碱基为G或A；
BrSNPA02036位于A02号染色体上第17828400位，其碱基为T或C；
BrSNPA02037位于A02号染色体上第17930830位，其碱基为T或C；
BrSNPA02038位于A02号染色体上第17961160位，其碱基为G或A；
BrSNPA02039位于A02号染色体上第18267114位，其碱基为T或C；
BrSNPA02040位于A02号染色体上第18364714位，其碱基为G或A；
BrSNPA02041位于A02号染色体上第18365894位，其碱基为T或G；
BrSNPA02042位于A02号染色体上第18425637位，其碱基为G或C；
BrSNPA02043位于A02号染色体上第19966721位，其碱基为T或A；
BrSNPA02044位于A02号染色体上第21426261位，其碱基为G或A；
BrSNPA02045位于A02号染色体上第21512340位，其碱基为G或A；
BrSNPA02046位于A02号染色体上第21970335位，其碱基为C或T；
BrSNPA02047位于A02号染色体上第22610904位，其碱基为T或C；
BrSNPA02048位于A02号染色体上第22844235位，其碱基为T或G；
BrSNPA02049位于A02号染色体上第22844925位，其碱基为G或A；
BrSNPA02050位于A02号染色体上第23028483位，其碱基为G或A；
BrSNPA02051位于A02号染色体上第23096858位，其碱基为T或C；
BrSNPA02052位于A02号染色体上第23174443位，其碱基为C或A；
BrSNPA02053位于A02号染色体上第23181920位，其碱基为G或C；
BrSNPA02054位于A02号染色体上第23632523位，其碱基为G或A；
BrSNPA02055位于A02号染色体上第24627499位，其碱基为A或G；
BrSNPA02056位于A02号染色体上第24987897位，其碱基为C或T；
BrSNPA02057位于A02号染色体上第25360564位，其碱基为C或A；
BrSNPA02058位于A02号染色体上第25748039位，其碱基为G或A；
BrSNPA02059位于A02号染色体上第26812976位，其碱基为G或C；
BrSNPA03001位于A03号染色体上第128271位，其碱基为C或A；
BrSNPA03002位于A03号染色体上第1038560位，其碱基为T或A；
BrSNPA03003位于A03号染色体上第1113130位，其碱基为T或C；
BrSNPA03004位于A03号染色体上第1964460位，其碱基为G或A；
BrSNPA03005位于A03号染色体上第2531794位，其碱基为T或C；
BrSNPA03006位于A03号染色体上第2904270位，其碱基为T或G；
BrSNPA03007位于A03号染色体上第3151130位，其碱基为G或A；
BrSNPA03008位于A03号染色体上第3151142位，其碱基为T或C；
BrSNPA03009位于A03号染色体上第3230367位，其碱基为T或C；
BrSNPA03010位于A03号染色体上第3596283位，其碱基为C或G；
BrSNPA03011位于A03号染色体上第3671765位，其碱基为T或C；
BrSNPA03012位于A03号染色体上第4366708位，其碱基为C或A；
BrSNPA03013位于A03号染色体上第4417310位，其碱基为T或G；
BrSNPA03014位于A03号染色体上第4471683位，其碱基为G或A；
BrSNPA03015位于A03号染色体上第5211812位，其碱基为A或T；
BrSNPA03016位于A03号染色体上第5450320位，其碱基为C或A；
BrSNPA03017位于A03号染色体上第6359471位，其碱基为G或A；
BrSNPA03018位于A03号染色体上第7120485位，其碱基为T或C；
BrSNPA03019位于A03号染色体上第7461075位，其碱基为G或C；
BrSNPA03020位于A03号染色体上第7482663位，其碱基为T或C；
BrSNPA03021位于A03号染色体上第7645227位，其碱基为T或C；
BrSNPA03022位于A03号染色体上第7828461位，其碱基为G或A；
BrSNPA03023位于A03号染色体上第7962179位，其碱基为G或A；
BrSNPA03024位于A03号染色体上第8473550位，其碱基为G或A；
BrSNPA03025位于A03号染色体上第8903849位，其碱基为T或A；
BrSNPA03026位于A03号染色体上第8939875位，其碱基为T或C；
BrSNPA03027位于A03号染色体上第9140011位，其碱基为G或A；
BrSNPA03028位于A03号染色体上第9143142位，其碱基为C或A；
BrSNPA03029位于A03号染色体上第10375219位，其碱基为T或G；
BrSNPA03030位于A03号染色体上第10697749位，其碱基为A或G；
BrSNPA03031位于A03号染色体上第10918916位，其碱基为T或G；
BrSNPA03032位于A03号染色体上第11197981位，其碱基为G或C；
BrSNPA03033位于A03号染色体上第11383704位，其碱基为T或C；
BrSNPA03034位于A03号染色体上第11557175位，其碱基为C或T；
BrSNPA03035位于A03号染色体上第11754718位，其碱基为G或A；
BrSNPA03036位于A03号染色体上第12070633位，其碱基为G或A；
BrSNPA03037位于A03号染色体上第12523505位，其碱基为G或C；
BrSNPA03038位于A03号染色体上第12806173位，其碱基为T或A；
BrSNPA03039位于A03号染色体上第13392725位，其碱基为G或A；
BrSNPA03040位于A03号染色体上第14206073位，其碱基为C或T；
BrSNPA03041位于A03号染色体上第14660690位，其碱基为G或A；
BrSNPA03042位于A03号染色体上第14844334位，其碱基为G或C；
BrSNPA03043位于A03号染色体上第15312198位，其碱基为T或G；
BrSNPA03044位于A03号染色体上第15431305位，其碱基为G或C；
BrSNPA03045位于A03号染色体上第16097389位，其碱基为G或A；
BrSNPA03046位于A03号染色体上第16144120位，其碱基为G或C；
BrSNPA03047位于A03号染色体上第17940082位，其碱基为T或G；
BrSNPA03048位于A03号染色体上第18277222位，其碱基为T或A；
BrSNPA03049位于A03号染色体上第19649018位，其碱基为A或G；
BrSNPA03050位于A03号染色体上第19734725位，其碱基为T或A；
BrSNPA03051位于A03号染色体上第19973528位，其碱基为T或C；
BrSNPA03052位于A03号染色体上第20233641位，其碱基为T或G；
BrSNPA03053位于A03号染色体上第20593495位，其碱基为A或T；
BrSNPA03054位于A03号染色体上第21003111位，其碱基为G或C；
BrSNPA03055位于A03号染色体上第21624403位，其碱基为T或G；
BrSNPA03056位于A03号染色体上第22632354位，其碱基为G或A；
BrSNPA03057位于A03号染色体上第23328550位，其碱基为C或A；
BrSNPA03058位于A03号染色体上第23335563位，其碱基为T或C；
BrSNPA03059位于A03号染色体上第23890389位，其碱基为A或G；
BrSNPA03060位于A03号染色体上第24086704位，其碱基为G或A；
BrSNPA03061位于A03号染色体上第24127049位，其碱基为T或A；
BrSNPA03062位于A03号染色体上第24352728位，其碱基为T或A；
BrSNPA03063位于A03号染色体上第25697075位，其碱基为T或A；
BrSNPA03064位于A03号染色体上第26316567位，其碱基为T或G；
BrSNPA03065位于A03号染色体上第26753682位，其碱基为G或A；
BrSNPA03066位于A03号染色体上第27196183位，其碱基为T或G；
BrSNPA03067位于A03号染色体上第27211545位，其碱基为C或A；
BrSNPA03068位于A03号染色体上第27471408位，其碱基为T或G；
BrSNPA03069位于A03号染色体上第28161015位，其碱基为G或C；
BrSNPA03070位于A03号染色体上第28193409位，其碱基为G或C；
BrSNPA03071位于A03号染色体上第28321741位，其碱基为G或A；
BrSNPA03072位于A03号染色体上第28629888位，其碱基为T或G；
BrSNPA03073位于A03号染色体上第28687095位，其碱基为G或A；
BrSNPA03074位于A03号染色体上第29060156位，其碱基为G或A；
BrSNPA03075位于A03号染色体上第29070757位，其碱基为C或A；
BrSNPA03076位于A03号染色体上第29086065位，其碱基为G或C；
BrSNPA03077位于A03号染色体上第30080620位，其碱基为T或C；
BrSNPA03078位于A03号染色体上第30648246位，其碱基为A或C；
BrSNPA04001位于A04号染色体上第556322位，其碱基为G或A；
BrSNPA04002位于A04号染色体上第898850位，其碱基为T或G；
BrSNPA04003位于A04号染色体上第1223515位，其碱基为G或A；
BrSNPA04004位于A04号染色体上第1378190位，其碱基为G或A；
BrSNPA04005位于A04号染色体上第1580901位，其碱基为T或C；
BrSNPA04006位于A04号染色体上第1661398位，其碱基为G或A；
BrSNPA04007位于A04号染色体上第1743434位，其碱基为G或A；
BrSNPA04008位于A04号染色体上第1833604位，其碱基为T或G；
BrSNPA04009位于A04号染色体上第2121284位，其碱基为G或A；
BrSNPA04010位于A04号染色体上第2232565位，其碱基为T或A；
BrSNPA04011位于A04号染色体上第2827196位，其碱基为G或A；
BrSNPA04012位于A04号染色体上第3162294位，其碱基为G或A；
BrSNPA04013位于A04号染色体上第3232728位，其碱基为G或C；
BrSNPA04014位于A04号染色体上第3760068位，其碱基为G或A；
BrSNPA04015位于A04号染色体上第3908277位，其碱基为G或A；
BrSNPA04016位于A04号染色体上第4437696位，其碱基为C或G；
BrSNPA04017位于A04号染色体上第4689758位，其碱基为T或C；
BrSNPA04018位于A04号染色体上第5060447位，其碱基为T或G；
BrSNPA04019位于A04号染色体上第6847805位，其碱基为G或C；
BrSNPA04020位于A04号染色体上第6933634位，其碱基为G或A；
BrSNPA04021位于A04号染色体上第8477239位，其碱基为A或C；
BrSNPA04022位于A04号染色体上第8662680位，其碱基为T或G；
BrSNPA04023位于A04号染色体上第9741929位，其碱基为G或T；
BrSNPA04024位于A04号染色体上第10563277位，其碱基为T或G；
BrSNPA04025位于A04号染色体上第11311194位，其碱基为G或C；
BrSNPA04026位于A04号染色体上第11642806位，其碱基为G或A；
BrSNPA04027位于A04号染色体上第11984195位，其碱基为T或G；
BrSNPA04028位于A04号染色体上第12352714位，其碱基为T或A；
BrSNPA04029位于A04号染色体上第12579896位，其碱基为G或A；
BrSNPA04030位于A04号染色体上第13764622位，其碱基为A或G；
BrSNPA04031位于A04号染色体上第14413900位，其碱基为G或A；
BrSNPA04032位于A04号染色体上第15528641位，其碱基为G或A；
BrSNPA04033位于A04号染色体上第15560443位，其碱基为T或C；
BrSNPA04034位于A04号染色体上第15732203位，其碱基为G或C；
BrSNPA04035位于A04号染色体上第16065091位，其碱基为T或A；
BrSNPA04036位于A04号染色体上第16331059位，其碱基为G或C；
BrSNPA04037位于A04号染色体上第16851784位，其碱基为T或C；
BrSNPA04038位于A04号染色体上第17018800位，其碱基为G或C；
BrSNPA04039位于A04号染色体上第18819192位，其碱基为T或C；
BrSNPA04040位于A04号染色体上第19072439位，其碱基为T或C；
BrSNPA04041位于A04号染色体上第19212412位，其碱基为G或A；
BrSNPA05001位于A05号染色体上第487586位，其碱基为A或T；
BrSNPA05002位于A05号染色体上第1283352位，其碱基为C或T；
BrSNPA05003位于A05号染色体上第2024918位，其碱基为G或A；
BrSNPA05004位于A05号染色体上第2253083位，其碱基为T或G；
BrSNPA05005位于A05号染色体上第2688397位，其碱基为G或C；
BrSNPA05006位于A05号染色体上第2809229位，其碱基为C或A；
BrSNPA05007位于A05号染色体上第2835432位，其碱基为G或A；
BrSNPA05008位于A05号染色体上第2945096位，其碱基为C或A；
BrSNPA05009位于A05号染色体上第3063334位，其碱基为G或T；
BrSNPA05010位于A05号染色体上第3608353位，其碱基为T或A；
BrSNPA05011位于A05号染色体上第3663635位，其碱基为T或C；
BrSNPA05012位于A05号染色体上第3669632位，其碱基为G或A；
BrSNPA05013位于A05号染色体上第3702768位，其碱基为T或A；
BrSNPA05014位于A05号染色体上第3711749位，其碱基为T或C；
BrSNPA05015位于A05号染色体上第4073949位，其碱基为G或A；
BrSNPA05016位于A05号染色体上第4227921位，其碱基为G或C；
BrSNPA05017位于A05号染色体上第4232210位，其碱基为G或A；
BrSNPA05018位于A05号染色体上第4379811位，其碱基为T或A；
BrSNPA05019位于A05号染色体上第5944167位，其碱基为T或A；
BrSNPA05020位于A05号染色体上第6796365位，其碱基为T或G；
BrSNPA05021位于A05号染色体上第7357437位，其碱基为C或A；
BrSNPA05022位于A05号染色体上第7531884位，其碱基为G或A；
BrSNPA05023位于A05号染色体上第7551654位，其碱基为G或A；
BrSNPA05024位于A05号染色体上第8088873位，其碱基为C或T；
BrSNPA05025位于A05号染色体上第8959623位，其碱基为G或A；
BrSNPA05026位于A05号染色体上第9401373位，其碱基为T或C；
BrSNPA05027位于A05号染色体上第9714592位，其碱基为G或C；
BrSNPA05028位于A05号染色体上第9832276位，其碱基为T或C；
BrSNPA05029位于A05号染色体上第9890212位，其碱基为T或A；
BrSNPA05030位于A05号染色体上第10343678位，其碱基为G或A；
BrSNPA05031位于A05号染色体上第10705210位，其碱基为C或A；
BrSNPA05032位于A05号染色体上第10722668位，其碱基为T或C；
BrSNPA05033位于A05号染色体上第10887243位，其碱基为T或C；
BrSNPA05034位于A05号染色体上第14441564位，其碱基为G或A；
BrSNPA05035位于A05号染色体上第14794179位，其碱基为T或C；
BrSNPA05036位于A05号染色体上第14801567位，其碱基为T或G；
BrSNPA05037位于A05号染色体上第14960042位，其碱基为G或C；
BrSNPA05038位于A05号染色体上第16182627位，其碱基为T或A；
BrSNPA05039位于A05号染色体上第16282153位，其碱基为T或C；
BrSNPA05040位于A05号染色体上第16543992位，其碱基为T或C；
BrSNPA05041位于A05号染色体上第16988803位，其碱基为T或A；
BrSNPA05042位于A05号染色体上第17247727位，其碱基为T或A；
BrSNPA05043位于A05号染色体上第17810370位，其碱基为G或A；
BrSNPA05044位于A05号染色体上第18626934位，其碱基为T或G；
BrSNPA05045位于A05号染色体上第19009544位，其碱基为G或C；
BrSNPA05046位于A05号染色体上第19022188位，其碱基为T或C；
BrSNPA05047位于A05号染色体上第20978461位，其碱基为C或A；
BrSNPA05048位于A05号染色体上第21254133位，其碱基为C或A；
BrSNPA05049位于A05号染色体上第21498528位，其碱基为G或A；
BrSNPA05050位于A05号染色体上第22030048位，其碱基为C或T；
BrSNPA05051位于A05号染色体上第22731111位，其碱基为G或C；
BrSNPA05052位于A05号染色体上第22895070位，其碱基为C或A；
BrSNPA05053位于A05号染色体上第22962829位，其碱基为T或C；
BrSNPA06001位于A06号染色体上第18887位，其碱基为C或G；
BrSNPA06002位于A06号染色体上第117846位，其碱基为A或G；
BrSNPA06003位于A06号染色体上第1296897位，其碱基为T或C；
BrSNPA06004位于A06号染色体上第1752922位，其碱基为G或A；
BrSNPA06005位于A06号染色体上第2426330位，其碱基为G或A；
BrSNPA06006位于A06号染色体上第2789766位，其碱基为T或G；
BrSNPA06007位于A06号染色体上第2931644位，其碱基为T或C；
BrSNPA06008位于A06号染色体上第6382083位，其碱基为C或A；
BrSNPA06009位于A06号染色体上第6403261位，其碱基为C或A；
BrSNPA06010位于A06号染色体上第8245454位，其碱基为G或A；
BrSNPA06011位于A06号染色体上第8618748位，其碱基为A或G；
BrSNPA06012位于A06号染色体上第10107459位，其碱基为G或A；
BrSNPA06013位于A06号染色体上第10568157位，其碱基为G或A；
BrSNPA06014位于A06号染色体上第10578879位，其碱基为T或C；
BrSNPA06015位于A06号染色体上第10874817位，其碱基为G或C；
BrSNPA06016位于A06号染色体上第11069822位，其碱基为T或G；
BrSNPA06017位于A06号染色体上第11344725位，其碱基为T或A；
BrSNPA06018位于A06号染色体上第12784985位，其碱基为T或C；
BrSNPA06019位于A06号染色体上第13424538位，其碱基为C或A；
BrSNPA06020位于A06号染色体上第13447511位，其碱基为G或A；
BrSNPA06021位于A06号染色体上第13534897位，其碱基为A或T；
BrSNPA06022位于A06号染色体上第13545093位，其碱基为T或C；
BrSNPA06023位于A06号染色体上第14701558位，其碱基为T或C；
BrSNPA06024位于A06号染色体上第14726472位，其碱基为C或A；
BrSNPA06025位于A06号染色体上第14730894位，其碱基为T或C；
BrSNPA06026位于A06号染色体上第14731133位，其碱基为T或A；
BrSNPA06027位于A06号染色体上第15606985位，其碱基为G或A；
BrSNPA06028位于A06号染色体上第15685596位，其碱基为G或A；
BrSNPA06029位于A06号染色体上第16018969位，其碱基为C或A；
BrSNPA06030位于A06号染色体上第16193195位，其碱基为G或C；
BrSNPA06031位于A06号染色体上第16201857位，其碱基为G或A；
BrSNPA06032位于A06号染色体上第16271874位，其碱基为T或A；
BrSNPA06033位于A06号染色体上第17483582位，其碱基为G或A；
BrSNPA06034位于A06号染色体上第17610866位，其碱基为A或G；
BrSNPA06035位于A06号染色体上第17773836位，其碱基为T或A；
BrSNPA06036位于A06号染色体上第18376779位，其碱基为G或A；
BrSNPA06037位于A06号染色体上第18546656位，其碱基为G或C；
BrSNPA06038位于A06号染色体上第18584746位，其碱基为G或A；
BrSNPA06039位于A06号染色体上第18685199位，其碱基为T或G；
BrSNPA06040位于A06号染色体上第19361503位，其碱基为G或C；
BrSNPA06041位于A06号染色体上第19387887位，其碱基为G或A；
BrSNPA06042位于A06号染色体上第20048140位，其碱基为G或A；
BrSNPA06043位于A06号染色体上第20833714位，其碱基为G或C；
BrSNPA06044位于A06号染色体上第20977558位，其碱基为T或C；
BrSNPA06045位于A06号染色体上第21415891位，其碱基为G或A；
BrSNPA06046位于A06号染色体上第21632076位，其碱基为G或A；
BrSNPA06047位于A06号染色体上第22374291位，其碱基为C或G；
BrSNPA06048位于A06号染色体上第23153503位，其碱基为C或A；
BrSNPA06049位于A06号染色体上第23563378位，其碱基为G或A；
BrSNPA06050位于A06号染色体上第23928146位，其碱基为G或A；
BrSNPA06051位于A06号染色体上第23955274位，其碱基为T或A；
BrSNPA06052位于A06号染色体上第24041088位，其碱基为C或T；
BrSNPA06053位于A06号染色体上第24284057位，其碱基为G或A；
BrSNPA06054位于A06号染色体上第24717886位，其碱基为C或A；
BrSNPA06055位于A06号染色体上第25147238位，其碱基为T或C；
BrSNPA07001位于A07号染色体上第246275位，其碱基为G或C；
BrSNPA07002位于A07号染色体上第641403位，其碱基为G或A；
BrSNPA07003位于A07号染色体上第1028346位，其碱基为T或A；
BrSNPA07004位于A07号染色体上第2560851位，其碱基为G或A；
BrSNPA07005位于A07号染色体上第2970833位，其碱基为A或G；
BrSNPA07006位于A07号染色体上第3216036位，其碱基为T或C；
BrSNPA07007位于A07号染色体上第3279889位，其碱基为T或G；
BrSNPA07008位于A07号染色体上第3358700位，其碱基为C或A；
BrSNPA07009位于A07号染色体上第3961792位，其碱基为T或A；
BrSNPA07010位于A07号染色体上第4234366位，其碱基为T或C；
BrSNPA07011位于A07号染色体上第5021185位，其碱基为T或G；
BrSNPA07012位于A07号染色体上第5153068位，其碱基为T或G；
BrSNPA07013位于A07号染色体上第6101124位，其碱基为T或A；
BrSNPA07014位于A07号染色体上第6254138位，其碱基为T或A；
BrSNPA07015位于A07号染色体上第6429733位，其碱基为T或C；
BrSNPA07016位于A07号染色体上第7129127位，其碱基为T或C；
BrSNPA07017位于A07号染色体上第7137784位，其碱基为G或A；
BrSNPA07018位于A07号染色体上第7249922位，其碱基为C或A；
BrSNPA07019位于A07号染色体上第8738315位，其碱基为T或G；
BrSNPA07020位于A07号染色体上第9057585位，其碱基为G或A；
BrSNPA07021位于A07号染色体上第9463049位，其碱基为T或C；
BrSNPA07022位于A07号染色体上第9678954位，其碱基为T或C；
BrSNPA07023位于A07号染色体上第11143455位，其碱基为T或C；
BrSNPA07024位于A07号染色体上第11295388位，其碱基为G或A；
BrSNPA07025位于A07号染色体上第11498098位，其碱基为T或G；
BrSNPA07026位于A07号染色体上第11520532位，其碱基为G或A；
BrSNPA07027位于A07号染色体上第11551826位，其碱基为T或C；
BrSNPA07028位于A07号染色体上第11750525位，其碱基为G或A；
BrSNPA07029位于A07号染色体上第12234015位，其碱基为C或T；
BrSNPA07030位于A07号染色体上第13554367位，其碱基为G或A；
BrSNPA07031位于A07号染色体上第14074310位，其碱基为T或C；
BrSNPA07032位于A07号染色体上第14523031位，其碱基为T或C；
BrSNPA07033位于A07号染色体上第14747998位，其碱基为T或A；
BrSNPA07034位于A07号染色体上第15655059位，其碱基为A或G；
BrSNPA07035位于A07号染色体上第15740558位，其碱基为C或A；
BrSNPA07036位于A07号染色体上第16130782位，其碱基为T或C；
BrSNPA07037位于A07号染色体上第16491110位，其碱基为T或G；
BrSNPA07038位于A07号染色体上第17285904位，其碱基为G或A；
BrSNPA07039位于A07号染色体上第18060124位，其碱基为T或G；
BrSNPA07040位于A07号染色体上第18453973位，其碱基为C或T；
BrSNPA07041位于A07号染色体上第19178191位，其碱基为T或C；
BrSNPA07042位于A07号染色体上第19761996位，其碱基为G或A；
BrSNPA07043位于A07号染色体上第19894600位，其碱基为T或C；
BrSNPA07044位于A07号染色体上第20112319位，其碱基为G或C；
BrSNPA07045位于A07号染色体上第21087891位，其碱基为A或G；
BrSNPA07046位于A07号染色体上第21941825位，其碱基为T或C；
BrSNPA07047位于A07号染色体上第22261109位，其碱基为T或G；
BrSNPA07048位于A07号染色体上第22291912位，其碱基为T或C；
BrSNPA07049位于A07号染色体上第22522822位，其碱基为C或T；
BrSNPA07050位于A07号染色体上第23348479位，其碱基为G或A；
BrSNPA07051位于A07号染色体上第24460147位，其碱基为G或C；
BrSNPA07052位于A07号染色体上第25407338位，其碱基为T或A；
BrSNPA07053位于A07号染色体上第25633222位，其碱基为G或A；
BrSNPA07054位于A07号染色体上第25744414位，其碱基为A或C；
BrSNPA08001位于A08号染色体上第100717位，其碱基为C或G；
BrSNPA08002位于A08号染色体上第240722位，其碱基为G或A；
BrSNPA08003位于A08号染色体上第277090位，其碱基为T或C；
BrSNPA08004位于A08号染色体上第926709位，其碱基为G或A；
BrSNPA08005位于A08号染色体上第1715093位，其碱基为G或A；
BrSNPA08006位于A08号染色体上第2121093位，其碱基为G或A；
BrSNPA08007位于A08号染色体上第2374634位，其碱基为G或A；
BrSNPA08008位于A08号染色体上第3289888位，其碱基为T或G；
BrSNPA08009位于A08号染色体上第3422941位，其碱基为C或G；
BrSNPA08010位于A08号染色体上第3911814位，其碱基为T或G；
BrSNPA08011位于A08号染色体上第3915045位，其碱基为T或C；
BrSNPA08012位于A08号染色体上第4419556位，其碱基为C或T；
BrSNPA08013位于A08号染色体上第5430112位，其碱基为T或G；
BrSNPA08014位于A08号染色体上第6266345位，其碱基为C或A；
BrSNPA08015位于A08号染色体上第7150432位，其碱基为G或A；
BrSNPA08016位于A08号染色体上第7588851位，其碱基为T或C；
BrSNPA08017位于A08号染色体上第8135981位，其碱基为C或A；
BrSNPA08018位于A08号染色体上第8933259位，其碱基为G或A；
BrSNPA08019位于A08号染色体上第9415744位，其碱基为T或G；
BrSNPA08020位于A08号染色体上第10869244位，其碱基为C或T；
BrSNPA08021位于A08号染色体上第11195840位，其碱基为T或A；
BrSNPA08022位于A08号染色体上第12382048位，其碱基为T或A；
BrSNPA08023位于A08号染色体上第12394480位，其碱基为T或A；
BrSNPA08024位于A08号染色体上第12855208位，其碱基为C或A；
BrSNPA08025位于A08号染色体上第12939081位，其碱基为G或C；
BrSNPA08026位于A08号染色体上第13838879位，其碱基为T或A；
BrSNPA08027位于A08号染色体上第14102664位，其碱基为G或C；
BrSNPA08028位于A08号染色体上第14112886位，其碱基为T或C；
BrSNPA08029位于A08号染色体上第14137581位，其碱基为T或C；
BrSNPA08030位于A08号染色体上第14580308位，其碱基为T或C；
BrSNPA08031位于A08号染色体上第14960288位，其碱基为G或C；
BrSNPA08032位于A08号染色体上第15007562位，其碱基为T或G；
BrSNPA08033位于A08号染色体上第15287418位，其碱基为G或C；
BrSNPA08034位于A08号染色体上第15734124位，其碱基为G或A；
BrSNPA08035位于A08号染色体上第16162051位，其碱基为T或G；
BrSNPA08036位于A08号染色体上第16415364位，其碱基为T或A；
BrSNPA08037位于A08号染色体上第16583368位，其碱基为T或C；
BrSNPA08038位于A08号染色体上第17179902位，其碱基为T或G；
BrSNPA08039位于A08号染色体上第17537125位，其碱基为T或C；
BrSNPA08040位于A08号染色体上第17877117位，其碱基为T或C；
BrSNPA08041位于A08号染色体上第18085207位，其碱基为T或C；
BrSNPA08042位于A08号染色体上第18668405位，其碱基为T或C；
BrSNPA08043位于A08号染色体上第19658257位，其碱基为G或A；
BrSNPA08044位于A08号染色体上第19860954位，其碱基为C或T；
BrSNPA08045位于A08号染色体上第19861288位，其碱基为C或T；
BrSNPA08046位于A08号染色体上第20202413位，其碱基为G或C；
BrSNPA08047位于A08号染色体上第20416840位，其碱基为G或A；
BrSNPA08048位于A08号染色体上第20458612位，其碱基为T或C；
BrSNPA08049位于A08号染色体上第20716882位，其碱基为G或C；
BrSNPA09001位于A09号染色体上第165179位，其碱基为G或C；
BrSNPA09002位于A09号染色体上第211286位，其碱基为T或A；
BrSNPA09003位于A09号染色体上第443160位，其碱基为T或A；
BrSNPA09004位于A09号染色体上第583658位，其碱基为A或G；
BrSNPA09005位于A09号染色体上第898133位，其碱基为T或A；
BrSNPA09006位于A09号染色体上第1161182位，其碱基为T或C；
BrSNPA09007位于A09号染色体上第1379762位，其碱基为G或A；
BrSNPA09008位于A09号染色体上第1595348位，其碱基为T或G；
BrSNPA09009位于A09号染色体上第1627618位，其碱基为G或C；
BrSNPA09010位于A09号染色体上第1901646位，其碱基为T或A；
BrSNPA09011位于A09号染色体上第1930677位，其碱基为G或C；
BrSNPA09012位于A09号染色体上第3019969位，其碱基为G或T；
BrSNPA09013位于A09号染色体上第3817906位，其碱基为G或C；
BrSNPA09014位于A09号染色体上第3896403位，其碱基为G或A；
BrSNPA09015位于A09号染色体上第3903092位，其碱基为G或C；
BrSNPA09016位于A09号染色体上第4226515位，其碱基为G或A；
BrSNPA09017位于A09号染色体上第4354836位，其碱基为C或A；
BrSNPA09018位于A09号染色体上第4534869位，其碱基为C或A；
BrSNPA09019位于A09号染色体上第4566148位，其碱基为G或A；
BrSNPA09020位于A09号染色体上第4597771位，其碱基为G或C；
BrSNPA09021位于A09号染色体上第5122605位，其碱基为C或A；
BrSNPA09022位于A09号染色体上第5480210位，其碱基为T或C；
BrSNPA09023位于A09号染色体上第5575779位，其碱基为T或G；
BrSNPA09024位于A09号染色体上第6031641位，其碱基为T或C；
BrSNPA09025位于A09号染色体上第6984636位，其碱基为T或C；
BrSNPA09026位于A09号染色体上第7624272位，其碱基为C或T；
BrSNPA09027位于A09号染色体上第7624862位，其碱基为G或T；
BrSNPA09028位于A09号染色体上第7897532位，其碱基为T或C；
BrSNPA09029位于A09号染色体上第8589991位，其碱基为G或T；
BrSNPA09030位于A09号染色体上第8773906位，其碱基为G或T；
BrSNPA09031位于A09号染色体上第8876252位，其碱基为T或A；
BrSNPA09032位于A09号染色体上第8877380位，其碱基为C或G；
BrSNPA09033位于A09号染色体上第9006311位，其碱基为C或A；
BrSNPA09034位于A09号染色体上第9062940位，其碱基为T或C；
BrSNPA09035位于A09号染色体上第9303625位，其碱基为T或G；
BrSNPA09036位于A09号染色体上第9805886位，其碱基为C或T；
BrSNPA09037位于A09号染色体上第10247545位，其碱基为C或A；
BrSNPA09038位于A09号染色体上第11056315位，其碱基为T或A；
BrSNPA09039位于A09号染色体上第11230572位，其碱基为G或A；
BrSNPA09040位于A09号染色体上第12895394位，其碱基为G或A；
BrSNPA09041位于A09号染色体上第13470942位，其碱基为T或C；
BrSNPA09042位于A09号染色体上第16265081位，其碱基为A或C；
BrSNPA09043位于A09号染色体上第17752348位，其碱基为G或C；
BrSNPA09044位于A09号染色体上第18332606位，其碱基为T或C；
BrSNPA09045位于A09号染色体上第18356344位，其碱基为G或A；
BrSNPA09046位于A09号染色体上第18406676位，其碱基为T或C；
BrSNPA09047位于A09号染色体上第18417691位，其碱基为T或G；
BrSNPA09048位于A09号染色体上第20994890位，其碱基为T或C；
BrSNPA09049位于A09号染色体上第21636788位，其碱基为G或A；
BrSNPA09050位于A09号染色体上第21654909位，其碱基为T或C；
BrSNPA09051位于A09号染色体上第21654935位，其碱基为T或C；
BrSNPA09052位于A09号染色体上第21910417位，其碱基为T或C；
BrSNPA09053位于A09号染色体上第22627850位，其碱基为G或A；
BrSNPA09054位于A09号染色体上第23220955位，其碱基为G或A；
BrSNPA09055位于A09号染色体上第23322031位，其碱基为G或A；
BrSNPA09056位于A09号染色体上第25785187位，其碱基为G或A；
BrSNPA09057位于A09号染色体上第26222141位，其碱基为T或C；
BrSNPA09058位于A09号染色体上第26503872位，其碱基为T或A；
BrSNPA09059位于A09号染色体上第26520788位，其碱基为G或C；
BrSNPA09060位于A09号染色体上第26564841位，其碱基为G或A；
BrSNPA09061位于A09号染色体上第26594548位，其碱基为T或A；
BrSNPA09062位于A09号染色体上第26739505位，其碱基为T或A；
BrSNPA09063位于A09号染色体上第26924828位，其碱基为G或C；
BrSNPA09064位于A09号染色体上第27270361位，其碱基为T或C；
BrSNPA09065位于A09号染色体上第28770187位，其碱基为T或C；
BrSNPA09066位于A09号染色体上第29198476位，其碱基为T或C；
BrSNPA09067位于A09号染色体上第30179678位，其碱基为G或A；
BrSNPA09068位于A09号染色体上第30448774位，其碱基为T或A；
BrSNPA09069位于A09号染色体上第30507943位，其碱基为T或C；
BrSNPA09070位于A09号染色体上第31873135位，其碱基为T或C；
BrSNPA09071位于A09号染色体上第31875876位，其碱基为T或C；
BrSNPA09072位于A09号染色体上第32719234位，其碱基为C或T；
BrSNPA09073位于A09号染色体上第32983605位，其碱基为G或A；
BrSNPA09074位于A09号染色体上第33495110位，其碱基为A或C；
BrSNPA09075位于A09号染色体上第34559009位，其碱基为G或A；
BrSNPA09076位于A09号染色体上第37047265位，其碱基为T或G；
BrSNPA09077位于A09号染色体上第37238042位，其碱基为C或A；
BrSNPA09078位于A09号染色体上第37689520位，其碱基为T或A；
BrSNPA09079位于A09号染色体上第37741739位，其碱基为G或A；
BrSNPA09080位于A09号染色体上第37743859位，其碱基为G或A；
BrSNPA10001位于A10号染色体上第9384位，其碱基为T或G；
BrSNPA10002位于A10号染色体上第119709位，其碱基为T或A；
BrSNPA10003位于A10号染色体上第617354位，其碱基为T或A；
BrSNPA10004位于A10号染色体上第1861569位，其碱基为T或C；
BrSNPA10005位于A10号染色体上第3689550位，其碱基为T或C；
BrSNPA10006位于A10号染色体上第3708065位，其碱基为G或A；
BrSNPA10007位于A10号染色体上第3721991位，其碱基为T或C；
BrSNPA10008位于A10号染色体上第3800101位，其碱基为G或A；
BrSNPA10009位于A10号染色体上第3834274位，其碱基为C或A；
BrSNPA10010位于A10号染色体上第3849310位，其碱基为C或A；
BrSNPA10011位于A10号染色体上第3878034位，其碱基为T或A；
BrSNPA10012位于A10号染色体上第4497856位，其碱基为T或A；
BrSNPA10013位于A10号染色体上第4782602位，其碱基为T或C；
BrSNPA10014位于A10号染色体上第5601251位，其碱基为G或C；
BrSNPA10015位于A10号染色体上第5671082位，其碱基为G或A；
BrSNPA10016位于A10号染色体上第6046254位，其碱基为G或A；
BrSNPA10017位于A10号染色体上第6294669位，其碱基为T或G；
BrSNPA10018位于A10号染色体上第6364310位，其碱基为T或G；
BrSNPA10019位于A10号染色体上第6477381位，其碱基为C或A；
BrSNPA10020位于A10号染色体上第7174178位，其碱基为G或C；
BrSNPA10021位于A10号染色体上第7989917位，其碱基为G或C；
BrSNPA10022位于A10号染色体上第8425118位，其碱基为T或C；
BrSNPA10023位于A10号染色体上第8648405位，其碱基为T或A；
BrSNPA10024位于A10号染色体上第9700540位，其碱基为G或C；
BrSNPA10025位于A10号染色体上第10373824位，其碱基为G或A；
BrSNPA10026位于A10号染色体上第10740098位，其碱基为T或C；
BrSNPA10027位于A10号染色体上第12134922位，其碱基为A或G；
BrSNPA10028位于A10号染色体上第12492205位，其碱基为G或C；
BrSNPA10029位于A10号染色体上第12527035位，其碱基为G或C；
BrSNPA10030位于A10号染色体上第12619426位，其碱基为G或A；
BrSNPA10031位于A10号染色体上第13105346位，其碱基为C或A；
BrSNPA10032位于A10号染色体上第13127812位，其碱基为G或T；
BrSNPA10033位于A10号染色体上第13406296位，其碱基为T或G；
BrSNPA10034位于A10号染色体上第13716056位，其碱基为T或A；
BrSNPA10035位于A10号染色体上第13732849位，其碱基为T或C；
BrSNPA10036位于A10号染色体上第13859895位，其碱基为G或A；
BrSNPA10037位于A10号染色体上第14319070位，其碱基为T或A；
BrSNPA10038位于A10号染色体上第14443918位，其碱基为C或A；
BrSNPA10039位于A10号染色体上第14465630位，其碱基为G或C；
BrSNPA10040位于A10号染色体上第14638538位，其碱基为C或A；
BrSNPA10041位于A10号染色体上第15240989位，其碱基为G或C；
BrSNPA10042位于A10号染色体上第15835648位，其碱基为G或C；
BrSNPA10043位于A10号染色体上第16198723位，其碱基为G或A。
实施例2、利用实施例1筛选的571个SNP位点设计用于检测对应SNP位点基因型的KASP引物组合并建立基因型检测方法
一、利用实施例1筛选的571个SNP位点设计用于检测对应SNP位点基因型的KASP引物组合
根据实施例1筛选的571个SNP位点设计用于检测对应SNP位点基因型KASP引物组合。设计检测所述571个SNP位点的571组KASP引物，每组KASP引物用于检测所述571个SNP位点中的一种。每组KASP引物有2条上游引物(记为上游引物1和上游引物2)和1条下游引物。
上游引物1的结构组成自5’端至3’端依次为：通用序列1+基因组特异序列+所述SNP位点处两种核苷酸中的一种；
上游引物2的结构组成自5’端至3’端依次为：通用序列2+基因组特异序列+所述SNP位点处两种核苷酸中的另一种；
下游引物仅由基因组特异序列组成。
即针对SNP位点，上游引物的3′端为等位变异碱基。
其中，本发明采用的通用序列1为FAM荧光标签序列5′-GAAGGTGACCAAGTTCATGCT-3′，通用序列2为HEX荧光标签序列5′-GAAGGTCGGAGTCAACGGATT-3′。
具体而言，本发明针对实施例1筛选的571个SNP位点设计的KASP引物组合由序列表中序列1-序列1713所示的1713条单链DNA组成。
其中，序列1、4、7、……、1171(即序列3N-2，其中N为大于等于1且小于等于571的整数)为针对所述571个SNP位点的上游引物1，自5’端起前21位核苷酸为通用序列1(FAM荧光标签序列)，其后的序列为基因组特异性序列，3’末端为对应SNP位点处两种核苷酸中的一种；序列2、5、8、……、1172(即序列3N-1，其中N为大于等于1且小于等于571的整数)为针对所述571个SNP位点的上游引物2，自5’端起前21位核苷酸为通用序列2(HEX荧光标签序列)，其后的序列为基因组特异性序列，3’末端为对应SNP位点处两种核苷酸中的另一种；序列3、6、9、……、1173(即序列3N，其中N为大于等于1且小于等于571的整数)为针对所述571个SNP位点的下游引物，为基因组特异性序列。序列1-3所示的3个引物用于检测SNP位点BrSNPA01001(实施例1中571个SNP位点中的第1个)；序列4-6所示的3个引物用于检测SNP位点BrSNPA01002(实施例1中571个SNP位点中的第2个)；序列7-9所示的3个引物用于检测SNP位点BrSNPA01003(实施例1中571个SNP位点中的第3个)；……，以此类推，……，序列1711-1713所示的3个引物用于检测SNP位点BrSNPA10043(实施例1中571个SNP位点中的第571个)。
二、利用KASP引物组合检测571个SNP位点基因型的方法的建立
1、基因组DNA的提取及质量鉴定
常规CTAB法分别提取供试白菜材料的基因组DNA。用琼脂糖电泳和Nanodrop2100分别检测所提取DNA的质量，发现提取的基因组DNA均达到了相关的质量要求，即琼脂糖电泳显示DNA条带单一，没有明显弥散；Nanodrop2100检测A260/280介于1.8-2.0之间(DNA样品没有蛋白污染)；A260/230介于1.8-2.0之间(DNA样品盐离子浓度低)；270nm没有明显的光吸收(DNA样品没有酚污染)；用于竞争性等位基因特异性PCR技术检测的DNA用量为4-10ng/每样品。稀释DNA浓度成为4-10ng/μl备用。
2、PCR扩增
以步骤一提取的基因组DNA为模板，用实施例1开发的KASP引物组合中的每种KASP引物分别进行PCR扩增，得到PCR扩增产物。
按照英国LGC(Laboratory of the Government Chemist政府化学家实验室)有限公司提供的标准实验流程，即基于竞争性等位基因特异性PCR技术的实验流程进行实验，以下试剂除特殊说明为均为LGC公司提供的配套试剂，试剂用量、用法、以及整个实验步骤按照LGC公司的操作指南KASP user guide and manual(www.lgcgenomics.com)进行：
KASPar反应在384微孔板(Part No.KBS-0750-001)或者1536微孔板(Part No.KBS-0751-001)中进行，反应体系为3μl或1μl。
具体步骤为：首先利用K-pette分液工作站在微孔板中加入待测DNA模板 (4～10ng/μl)1.5μl，60℃烘干。然后在Kraken操作系统下利用Meridian加样工作站向每个反应孔中加入终浓度为1×的Master mix(将Part No.KBS-1016-002或者Part No.KBS-1016-011稀释后所得)与引物预混液(两条上游引物和下游引物按照摩尔浓度比6:6:15混合，各引物终浓度为10μM)，Mix分装完毕立即将微孔板依次放在Kube热封仪和Fusion激光封膜仪上封膜。
PCR反应在高通量水浴系统Hydrocycler中进行，具体程序为：94℃预变性15分钟；94℃变性20秒，61℃-55℃复性&延伸1分钟，以touch down程序扩增10个循环，复性&延伸温度每循环降低0.6℃；94℃变性20秒，55℃60秒，26个循环。
实验同时设置反应体系中不添加模板DNA的空白对照。每个PCR扩增产物样本设置至少3个重复。
4、等位基因分型
扩增结束后，利用BMG PHERAstar仪器检测荧光信号并查看分型情况。若分型不充分，则继续扩增，每3个循环查看分型情况，直至分型完全。
利用KrakenTM软件将原始数据导出为microsoft office excel2010格式，分析待测样品在571个位点的基因分型情况。
实施例3、利用实施例2设计的KASP引物组合检测571个SNP位点基因型构建白菜DNA指纹数据库
一、DNA提取及质量鉴定
供试白菜品种：255份不同白菜亚种和变种的代表性自交系样品(表1)。
参见实施例2步骤二1提取各供试白菜品种的基因组DNA。
二、571个SNP位点基因型的检测
采用实施例2设计的KASP引物组合对步骤1获得的各供试白菜品种的基因组DNA分别进行实施例1中571个SNP位点基因型的检测。具体操作方法参见实施例2步骤二。最终所得各供试白菜品种的571个SNP位点的基因型数据即构成白菜DNA指纹数据库。
实施例4、利用实施例3获得的SNP白菜DNA指纹数据库对白菜遗传多样性分析和进化分析
本发明提供的571个SNP位点在筛选之初，由于已经考虑到区分白菜各亚种及变种，因此可以用于白菜遗传资源分析和类群划分。
以下将详述利用实施例1-3获得的571个SNP位点的基因型数据对255份不同白菜亚种和变种(同实施例3)的代表性自交系样品进行类群划分。
基于实施例3获得的255份白菜样品571个SNP位点的基因型数据，利用Power-Marker ver.3.25软件，选用Nei1972遗传距离计算法，分析建立UPGMA法聚类树状图，255份样品被划分为到不同的群内。
结果如图2所示，从图中可以看出，芸薹种作物的四个亚种及更为细分的变 种，包括结球白菜Brassica rapa ssp.pekinensis(细分为秋播大白菜Aut(autumn-type)，夏播大白菜Sum(summer-type)，春播大白菜Spr(spring-type))，不结球白菜Brassica rapa ssp.chinensis(细分为青梗菜ssp.chinensis var.chinensis(C)，乌塌菜ssp.chinensis var.Narinosa(Nar)，菜薹ssp.chinensis var.parachinensis(P)，苔菜ssp.chinensis var.tsai-cai(T)，分蘖菜ssp.chinensis var.multiceps(J)，和奶白菜ssp.chinensis var.nai-bai(N)，ssp.chinensis var.hei-ye(H)共计7个变种)，油用白菜Brassica rapa ssp.oleifera(O)和芜菁Brassica rapa ssp.rapifera(R)都能完整区分出来。
实施例5、利用SNP分子标记进行白菜分子育种的背景选择
在白菜新品种选育过程中，通常将具有优良性状的自交系材料(供体)和综合性状较好的轮回亲本(受体)进行多代的回交，从而获得整体性状与轮回亲本相似，但增加了从供体转来的有利性状的后代。利用所述571个SNP位点可对回交育种过程中的中间材料进行背景选择，以达到快速获得整合了目标性状新品系的目的。
例如，在本发明中，亲本材料胶二叶(品种“胶二叶”自交六代以后所获自交系，文献来源：TuMV株系分化的研究.齐秀菊,卢和英,王子欣-《华北农学报》-1989)具有较好的综合农艺性状，但不抗干烧心病；亲本材料珍绿6号(品种“珍绿6号”自交六代以后所获自交系，珍绿6号可从天津科润农业科技股份有限公司购得)综合农艺性状一般，但干烧心病抗性突出。为得到综合性状优异并且干烧心抗性良好的新育种材料，本发明的发明人以将胶二叶(受体)和珍绿6号(供体)首先进行杂交，然后以胶二叶为轮回亲本进行选育。在F1BC3干烧心抗性良好材料中，利用571个SNP位点进行筛选。结果显示F1BC3材料T224和T20与胶二叶最为类似，相似程度分别达到99％和97.6％(图3)，后期的农艺性状观察发现T224和T20确实具有如胶二叶一般较好的综合农艺性状。
工业应用
本研究利用白菜重测序数据挖掘的SNP位点，基于KASP技术，在国际上首次开发了全基因组覆盖、高密度、重复率高、扩增稳定的571对引物。利用该引物组合可快速实现对已有种质资源遗传多样性的鉴定及个体精确分类；同时，通过遗传分析和种群划分预测杂种优势，这使得我们在杂交育种亲本选配过程中，避开亲缘关系较近的亲本从而提高后代获得杂交优势的概率。此外，随着我国白菜育种工作的快速发展，每年新推出的品种数以百计，我们迫切需要开发新一代的SNP分子标记技术，制定相应的品种审定和资源评价标准来为知识产权的保护提供强大的技术支持。利用本研究开发的标记，可在分子水平上鉴定品种纯度和真伪，具有准确可靠、成本低、高通量等突出优点；同时，由于开发了全基因组覆盖的高密度SNP分子标记，与以往几个或几十个标记的鉴定结果相比，其鉴定准确度显著提高。

Claims (15)

  1. 571个SNP位点或用于检测所述571个SNP位点的物质在如下任一中的应用：
    (1)白菜种质资源遗传多样性分析和/或进化分析；
    (2)白菜分子育种；
    (3)构建白菜DNA指纹数据库；
    (4)对白菜进行基因分型；
    (5)检测任两个白菜品种的相似性；
    所述571个SNP位点的物理位置是基于白菜品种Chiifu-402-2的全基因组序列比对确定的，所述白菜品种Chiifu-402-2的全基因组序列的版本号为V1.5；所述571个SNP位点如下：
    BrSNPA01001位于A01号染色体上第612144位，其碱基为T或A；
    BrSNPA01002位于A01号染色体上第1059908位，其碱基为T或C；
    BrSNPA01003位于A01号染色体上第1248141位，其碱基为T或C；
    BrSNPA01004位于A01号染色体上第1385646位，其碱基为T或A；
    BrSNPA01005位于A01号染色体上第1795429位，其碱基为T或C；
    BrSNPA01006位于A01号染色体上第2112068位，其碱基为A或T；
    BrSNPA01007位于A01号染色体上第2437505位，其碱基为G或A；
    BrSNPA01008位于A01号染色体上第2830301位，其碱基为G或A；
    BrSNPA01009位于A01号染色体上第2969334位，其碱基为G或A；
    BrSNPA01010位于A01号染色体上第2970196位，其碱基为T或C；
    BrSNPA01011位于A01号染色体上第3520140位，其碱基为T或C；
    BrSNPA01012位于A01号染色体上第4182701位，其碱基为T或C；
    BrSNPA01013位于A01号染色体上第4219165位，其碱基为C或A；
    BrSNPA01014位于A01号染色体上第4225329位，其碱基为T或G；
    BrSNPA01015位于A01号染色体上第4878177位，其碱基为T或C；
    BrSNPA01016位于A01号染色体上第5849627位，其碱基为T或G；
    BrSNPA01017位于A01号染色体上第6000315位，其碱基为C或G；
    BrSNPA01018位于A01号染色体上第6385755位，其碱基为T或C；
    BrSNPA01019位于A01号染色体上第7102966位，其碱基为T或A；
    BrSNPA01020位于A01号染色体上第7105232位，其碱基为G或A；
    BrSNPA01021位于A01号染色体上第7567812位，其碱基为T或C；
    BrSNPA01022位于A01号染色体上第8475689位，其碱基为C或T；
    BrSNPA01023位于A01号染色体上第9716212位，其碱基为G或C；
    BrSNPA01024位于A01号染色体上第9865712位，其碱基为T或A；
    BrSNPA01025位于A01号染色体上第10433882位，其碱基为A或G；
    BrSNPA01026位于A01号染色体上第10454017位，其碱基为T或C；
    BrSNPA01027位于A01号染色体上第11039761位，其碱基为T或C；
    BrSNPA01028位于A01号染色体上第11576989位，其碱基为G或A；
    BrSNPA01029位于A01号染色体上第12681321位，其碱基为T或G；
    BrSNPA01030位于A01号染色体上第12708200位，其碱基为T或C；
    BrSNPA01031位于A01号染色体上第12834470位，其碱基为G或C；
    BrSNPA01032位于A01号染色体上第13128980位，其碱基为T或C；
    BrSNPA01033位于A01号染色体上第14048162位，其碱基为T或C；
    BrSNPA01034位于A01号染色体上第14897192位，其碱基为C或T；
    BrSNPA01035位于A01号染色体上第15956879位，其碱基为T或C；
    BrSNPA01036位于A01号染色体上第16592150位，其碱基为G或C；
    BrSNPA01037位于A01号染色体上第17311260位，其碱基为T或C；
    BrSNPA01038位于A01号染色体上第18199499位，其碱基为T或C；
    BrSNPA01039位于A01号染色体上第18291203位，其碱基为T或C；
    BrSNPA01040位于A01号染色体上第18635734位，其碱基为G或A；
    BrSNPA01041位于A01号染色体上第18650317位，其碱基为G或A；
    BrSNPA01042位于A01号染色体上第18657425位，其碱基为T或C；
    BrSNPA01043位于A01号染色体上第18964955位，其碱基为G或C；
    BrSNPA01044位于A01号染色体上第20057539位，其碱基为G或A；
    BrSNPA01045位于A01号染色体上第21096109位，其碱基为C或A；
    BrSNPA01046位于A01号染色体上第21986324位，其碱基为G或A；
    BrSNPA01047位于A01号染色体上第22365926位，其碱基为G或C；
    BrSNPA01048位于A01号染色体上第22417219位，其碱基为G或A；
    BrSNPA01049位于A01号染色体上第22449302位，其碱基为G或A；
    BrSNPA01050位于A01号染色体上第22523311位，其碱基为C或T；
    BrSNPA01051位于A01号染色体上第22600539位，其碱基为T或C；
    BrSNPA01052位于A01号染色体上第22695943位，其碱基为C或A；
    BrSNPA01053位于A01号染色体上第24361302位，其碱基为T或C；
    BrSNPA01054位于A01号染色体上第24456539位，其碱基为G或T；
    BrSNPA01055位于A01号染色体上第24937773位，其碱基为G或A；
    BrSNPA01056位于A01号染色体上第25108874位，其碱基为T或C；
    BrSNPA01057位于A01号染色体上第26035077位，其碱基为T或A；
    BrSNPA01058位于A01号染色体上第26549708位，其碱基为C或T；
    BrSNPA01059位于A01号染色体上第26752589位，其碱基为G或A；
    BrSNPA02001位于A02号染色体上第356794位，其碱基为C或T；
    BrSNPA02002位于A02号染色体上第751652位，其碱基为G或A；
    BrSNPA02003位于A02号染色体上第884677位，其碱基为T或A；
    BrSNPA02004位于A02号染色体上第1026373位，其碱基为T或C；
    BrSNPA02005位于A02号染色体上第1065889位，其碱基为T或A；
    BrSNPA02006位于A02号染色体上第1142477位，其碱基为T或C；
    BrSNPA02007位于A02号染色体上第2421548位，其碱基为A或C；
    BrSNPA02008位于A02号染色体上第2663267位，其碱基为T或C；
    BrSNPA02009位于A02号染色体上第2865799位，其碱基为T或C；
    BrSNPA02010位于A02号染色体上第3245711位，其碱基为C或A；
    BrSNPA02011位于A02号染色体上第3685320位，其碱基为G或A；
    BrSNPA02012位于A02号染色体上第3812885位，其碱基为T或G；
    BrSNPA02013位于A02号染色体上第4523362位，其碱基为T或A；
    BrSNPA02014位于A02号染色体上第5184035位，其碱基为T或C；
    BrSNPA02015位于A02号染色体上第6035955位，其碱基为T或G；
    BrSNPA02016位于A02号染色体上第6431221位，其碱基为A或C；
    BrSNPA02017位于A02号染色体上第6709555位，其碱基为C或A；
    BrSNPA02018位于A02号染色体上第6957629位，其碱基为G或A；
    BrSNPA02019位于A02号染色体上第8447338位，其碱基为T或C；
    BrSNPA02020位于A02号染色体上第9141887位，其碱基为G或C；
    BrSNPA02021位于A02号染色体上第9423555位，其碱基为T或C；
    BrSNPA02022位于A02号染色体上第9490758位，其碱基为G或A；
    BrSNPA02023位于A02号染色体上第11160802位，其碱基为C或A；
    BrSNPA02024位于A02号染色体上第11181828位，其碱基为T或A；
    BrSNPA02025位于A02号染色体上第11698932位，其碱基为T或A；
    BrSNPA02026位于A02号染色体上第11780457位，其碱基为T或A；
    BrSNPA02027位于A02号染色体上第13059562位，其碱基为G或A；
    BrSNPA02028位于A02号染色体上第13619247位，其碱基为G或C；
    BrSNPA02029位于A02号染色体上第14369430位，其碱基为T或A；
    BrSNPA02030位于A02号染色体上第14606869位，其碱基为T或C；
    BrSNPA02031位于A02号染色体上第14862673位，其碱基为T或C；
    BrSNPA02032位于A02号染色体上第15736514位，其碱基为G或A；
    BrSNPA02033位于A02号染色体上第16465013位，其碱基为G或C；
    BrSNPA02034位于A02号染色体上第17440083位，其碱基为T或C；
    BrSNPA02035位于A02号染色体上第17778839位，其碱基为G或A；
    BrSNPA02036位于A02号染色体上第17828400位，其碱基为T或C；
    BrSNPA02037位于A02号染色体上第17930830位，其碱基为T或C；
    BrSNPA02038位于A02号染色体上第17961160位，其碱基为G或A；
    BrSNPA02039位于A02号染色体上第18267114位，其碱基为T或C；
    BrSNPA02040位于A02号染色体上第18364714位，其碱基为G或A；
    BrSNPA02041位于A02号染色体上第18365894位，其碱基为T或G；
    BrSNPA02042位于A02号染色体上第18425637位，其碱基为G或C；
    BrSNPA02043位于A02号染色体上第19966721位，其碱基为T或A；
    BrSNPA02044位于A02号染色体上第21426261位，其碱基为G或A；
    BrSNPA02045位于A02号染色体上第21512340位，其碱基为G或A；
    BrSNPA02046位于A02号染色体上第21970335位，其碱基为C或T；
    BrSNPA02047位于A02号染色体上第22610904位，其碱基为T或C；
    BrSNPA02048位于A02号染色体上第22844235位，其碱基为T或G；
    BrSNPA02049位于A02号染色体上第22844925位，其碱基为G或A；
    BrSNPA02050位于A02号染色体上第23028483位，其碱基为G或A；
    BrSNPA02051位于A02号染色体上第23096858位，其碱基为T或C；
    BrSNPA02052位于A02号染色体上第23174443位，其碱基为C或A；
    BrSNPA02053位于A02号染色体上第23181920位，其碱基为G或C；
    BrSNPA02054位于A02号染色体上第23632523位，其碱基为G或A；
    BrSNPA02055位于A02号染色体上第24627499位，其碱基为A或G；
    BrSNPA02056位于A02号染色体上第24987897位，其碱基为C或T；
    BrSNPA02057位于A02号染色体上第25360564位，其碱基为C或A；
    BrSNPA02058位于A02号染色体上第25748039位，其碱基为G或A；
    BrSNPA02059位于A02号染色体上第26812976位，其碱基为G或C；
    BrSNPA03001位于A03号染色体上第128271位，其碱基为C或A；
    BrSNPA03002位于A03号染色体上第1038560位，其碱基为T或A；
    BrSNPA03003位于A03号染色体上第1113130位，其碱基为T或C；
    BrSNPA03004位于A03号染色体上第1964460位，其碱基为G或A；
    BrSNPA03005位于A03号染色体上第2531794位，其碱基为T或C；
    BrSNPA03006位于A03号染色体上第2904270位，其碱基为T或G；
    BrSNPA03007位于A03号染色体上第3151130位，其碱基为G或A；
    BrSNPA03008位于A03号染色体上第3151142位，其碱基为T或C；
    BrSNPA03009位于A03号染色体上第3230367位，其碱基为T或C；
    BrSNPA03010位于A03号染色体上第3596283位，其碱基为C或G；
    BrSNPA03011位于A03号染色体上第3671765位，其碱基为T或C；
    BrSNPA03012位于A03号染色体上第4366708位，其碱基为C或A；
    BrSNPA03013位于A03号染色体上第4417310位，其碱基为T或G；
    BrSNPA03014位于A03号染色体上第4471683位，其碱基为G或A；
    BrSNPA03015位于A03号染色体上第5211812位，其碱基为A或T；
    BrSNPA03016位于A03号染色体上第5450320位，其碱基为C或A；
    BrSNPA03017位于A03号染色体上第6359471位，其碱基为G或A；
    BrSNPA03018位于A03号染色体上第7120485位，其碱基为T或C；
    BrSNPA03019位于A03号染色体上第7461075位，其碱基为G或C；
    BrSNPA03020位于A03号染色体上第7482663位，其碱基为T或C；
    BrSNPA03021位于A03号染色体上第7645227位，其碱基为T或C；
    BrSNPA03022位于A03号染色体上第7828461位，其碱基为G或A；
    BrSNPA03023位于A03号染色体上第7962179位，其碱基为G或A；
    BrSNPA03024位于A03号染色体上第8473550位，其碱基为G或A；
    BrSNPA03025位于A03号染色体上第8903849位，其碱基为T或A；
    BrSNPA03026位于A03号染色体上第8939875位，其碱基为T或C；
    BrSNPA03027位于A03号染色体上第9140011位，其碱基为G或A；
    BrSNPA03028位于A03号染色体上第9143142位，其碱基为C或A；
    BrSNPA03029位于A03号染色体上第10375219位，其碱基为T或G；
    BrSNPA03030位于A03号染色体上第10697749位，其碱基为A或G；
    BrSNPA03031位于A03号染色体上第10918916位，其碱基为T或G；
    BrSNPA03032位于A03号染色体上第11197981位，其碱基为G或C；
    BrSNPA03033位于A03号染色体上第11383704位，其碱基为T或C；
    BrSNPA03034位于A03号染色体上第11557175位，其碱基为C或T；
    BrSNPA03035位于A03号染色体上第11754718位，其碱基为G或A；
    BrSNPA03036位于A03号染色体上第12070633位，其碱基为G或A；
    BrSNPA03037位于A03号染色体上第12523505位，其碱基为G或C；
    BrSNPA03038位于A03号染色体上第12806173位，其碱基为T或A；
    BrSNPA03039位于A03号染色体上第13392725位，其碱基为G或A；
    BrSNPA03040位于A03号染色体上第14206073位，其碱基为C或T；
    BrSNPA03041位于A03号染色体上第14660690位，其碱基为G或A；
    BrSNPA03042位于A03号染色体上第14844334位，其碱基为G或C；
    BrSNPA03043位于A03号染色体上第15312198位，其碱基为T或G；
    BrSNPA03044位于A03号染色体上第15431305位，其碱基为G或C；
    BrSNPA03045位于A03号染色体上第16097389位，其碱基为G或A；
    BrSNPA03046位于A03号染色体上第16144120位，其碱基为G或C；
    BrSNPA03047位于A03号染色体上第17940082位，其碱基为T或G；
    BrSNPA03048位于A03号染色体上第18277222位，其碱基为T或A；
    BrSNPA03049位于A03号染色体上第19649018位，其碱基为A或G；
    BrSNPA03050位于A03号染色体上第19734725位，其碱基为T或A；
    BrSNPA03051位于A03号染色体上第19973528位，其碱基为T或C；
    BrSNPA03052位于A03号染色体上第20233641位，其碱基为T或G；
    BrSNPA03053位于A03号染色体上第20593495位，其碱基为A或T；
    BrSNPA03054位于A03号染色体上第21003111位，其碱基为G或C；
    BrSNPA03055位于A03号染色体上第21624403位，其碱基为T或G；
    BrSNPA03056位于A03号染色体上第22632354位，其碱基为G或A；
    BrSNPA03057位于A03号染色体上第23328550位，其碱基为C或A；
    BrSNPA03058位于A03号染色体上第23335563位，其碱基为T或C；
    BrSNPA03059位于A03号染色体上第23890389位，其碱基为A或G；
    BrSNPA03060位于A03号染色体上第24086704位，其碱基为G或A；
    BrSNPA03061位于A03号染色体上第24127049位，其碱基为T或A；
    BrSNPA03062位于A03号染色体上第24352728位，其碱基为T或A；
    BrSNPA03063位于A03号染色体上第25697075位，其碱基为T或A；
    BrSNPA03064位于A03号染色体上第26316567位，其碱基为T或G；
    BrSNPA03065位于A03号染色体上第26753682位，其碱基为G或A；
    BrSNPA03066位于A03号染色体上第27196183位，其碱基为T或G；
    BrSNPA03067位于A03号染色体上第27211545位，其碱基为C或A；
    BrSNPA03068位于A03号染色体上第27471408位，其碱基为T或G；
    BrSNPA03069位于A03号染色体上第28161015位，其碱基为G或C；
    BrSNPA03070位于A03号染色体上第28193409位，其碱基为G或C；
    BrSNPA03071位于A03号染色体上第28321741位，其碱基为G或A；
    BrSNPA03072位于A03号染色体上第28629888位，其碱基为T或G；
    BrSNPA03073位于A03号染色体上第28687095位，其碱基为G或A；
    BrSNPA03074位于A03号染色体上第29060156位，其碱基为G或A；
    BrSNPA03075位于A03号染色体上第29070757位，其碱基为C或A；
    BrSNPA03076位于A03号染色体上第29086065位，其碱基为G或C；
    BrSNPA03077位于A03号染色体上第30080620位，其碱基为T或C；
    BrSNPA03078位于A03号染色体上第30648246位，其碱基为A或C；
    BrSNPA04001位于A04号染色体上第556322位，其碱基为G或A；
    BrSNPA04002位于A04号染色体上第898850位，其碱基为T或G；
    BrSNPA04003位于A04号染色体上第1223515位，其碱基为G或A；
    BrSNPA04004位于A04号染色体上第1378190位，其碱基为G或A；
    BrSNPA04005位于A04号染色体上第1580901位，其碱基为T或C；
    BrSNPA04006位于A04号染色体上第1661398位，其碱基为G或A；
    BrSNPA04007位于A04号染色体上第1743434位，其碱基为G或A；
    BrSNPA04008位于A04号染色体上第1833604位，其碱基为T或G；
    BrSNPA04009位于A04号染色体上第2121284位，其碱基为G或A；
    BrSNPA04010位于A04号染色体上第2232565位，其碱基为T或A；
    BrSNPA04011位于A04号染色体上第2827196位，其碱基为G或A；
    BrSNPA04012位于A04号染色体上第3162294位，其碱基为G或A；
    BrSNPA04013位于A04号染色体上第3232728位，其碱基为G或C；
    BrSNPA04014位于A04号染色体上第3760068位，其碱基为G或A；
    BrSNPA04015位于A04号染色体上第3908277位，其碱基为G或A；
    BrSNPA04016位于A04号染色体上第4437696位，其碱基为C或G；
    BrSNPA04017位于A04号染色体上第4689758位，其碱基为T或C；
    BrSNPA04018位于A04号染色体上第5060447位，其碱基为T或G；
    BrSNPA04019位于A04号染色体上第6847805位，其碱基为G或C；
    BrSNPA04020位于A04号染色体上第6933634位，其碱基为G或A；
    BrSNPA04021位于A04号染色体上第8477239位，其碱基为A或C；
    BrSNPA04022位于A04号染色体上第8662680位，其碱基为T或G；
    BrSNPA04023位于A04号染色体上第9741929位，其碱基为G或T；
    BrSNPA04024位于A04号染色体上第10563277位，其碱基为T或G；
    BrSNPA04025位于A04号染色体上第11311194位，其碱基为G或C；
    BrSNPA04026位于A04号染色体上第11642806位，其碱基为G或A；
    BrSNPA04027位于A04号染色体上第11984195位，其碱基为T或G；
    BrSNPA04028位于A04号染色体上第12352714位，其碱基为T或A；
    BrSNPA04029位于A04号染色体上第12579896位，其碱基为G或A；
    BrSNPA04030位于A04号染色体上第13764622位，其碱基为A或G；
    BrSNPA04031位于A04号染色体上第14413900位，其碱基为G或A；
    BrSNPA04032位于A04号染色体上第15528641位，其碱基为G或A；
    BrSNPA04033位于A04号染色体上第15560443位，其碱基为T或C；
    BrSNPA04034位于A04号染色体上第15732203位，其碱基为G或C；
    BrSNPA04035位于A04号染色体上第16065091位，其碱基为T或A；
    BrSNPA04036位于A04号染色体上第16331059位，其碱基为G或C；
    BrSNPA04037位于A04号染色体上第16851784位，其碱基为T或C；
    BrSNPA04038位于A04号染色体上第17018800位，其碱基为G或C；
    BrSNPA04039位于A04号染色体上第18819192位，其碱基为T或C；
    BrSNPA04040位于A04号染色体上第19072439位，其碱基为T或C；
    BrSNPA04041位于A04号染色体上第19212412位，其碱基为G或A；
    BrSNPA05001位于A05号染色体上第487586位，其碱基为A或T；
    BrSNPA05002位于A05号染色体上第1283352位，其碱基为C或T；
    BrSNPA05003位于A05号染色体上第2024918位，其碱基为G或A；
    BrSNPA05004位于A05号染色体上第2253083位，其碱基为T或G；
    BrSNPA05005位于A05号染色体上第2688397位，其碱基为G或C；
    BrSNPA05006位于A05号染色体上第2809229位，其碱基为C或A；
    BrSNPA05007位于A05号染色体上第2835432位，其碱基为G或A；
    BrSNPA05008位于A05号染色体上第2945096位，其碱基为C或A；
    BrSNPA05009位于A05号染色体上第3063334位，其碱基为G或T；
    BrSNPA05010位于A05号染色体上第3608353位，其碱基为T或A；
    BrSNPA05011位于A05号染色体上第3663635位，其碱基为T或C；
    BrSNPA05012位于A05号染色体上第3669632位，其碱基为G或A；
    BrSNPA05013位于A05号染色体上第3702768位，其碱基为T或A；
    BrSNPA05014位于A05号染色体上第3711749位，其碱基为T或C；
    BrSNPA05015位于A05号染色体上第4073949位，其碱基为G或A；
    BrSNPA05016位于A05号染色体上第4227921位，其碱基为G或C；
    BrSNPA05017位于A05号染色体上第4232210位，其碱基为G或A；
    BrSNPA05018位于A05号染色体上第4379811位，其碱基为T或A；
    BrSNPA05019位于A05号染色体上第5944167位，其碱基为T或A；
    BrSNPA05020位于A05号染色体上第6796365位，其碱基为T或G；
    BrSNPA05021位于A05号染色体上第7357437位，其碱基为C或A；
    BrSNPA05022位于A05号染色体上第7531884位，其碱基为G或A；
    BrSNPA05023位于A05号染色体上第7551654位，其碱基为G或A；
    BrSNPA05024位于A05号染色体上第8088873位，其碱基为C或T；
    BrSNPA05025位于A05号染色体上第8959623位，其碱基为G或A；
    BrSNPA05026位于A05号染色体上第9401373位，其碱基为T或C；
    BrSNPA05027位于A05号染色体上第9714592位，其碱基为G或C；
    BrSNPA05028位于A05号染色体上第9832276位，其碱基为T或C；
    BrSNPA05029位于A05号染色体上第9890212位，其碱基为T或A；
    BrSNPA05030位于A05号染色体上第10343678位，其碱基为G或A；
    BrSNPA05031位于A05号染色体上第10705210位，其碱基为C或A；
    BrSNPA05032位于A05号染色体上第10722668位，其碱基为T或C；
    BrSNPA05033位于A05号染色体上第10887243位，其碱基为T或C；
    BrSNPA05034位于A05号染色体上第14441564位，其碱基为G或A；
    BrSNPA05035位于A05号染色体上第14794179位，其碱基为T或C；
    BrSNPA05036位于A05号染色体上第14801567位，其碱基为T或G；
    BrSNPA05037位于A05号染色体上第14960042位，其碱基为G或C；
    BrSNPA05038位于A05号染色体上第16182627位，其碱基为T或A；
    BrSNPA05039位于A05号染色体上第16282153位，其碱基为T或C；
    BrSNPA05040位于A05号染色体上第16543992位，其碱基为T或C；
    BrSNPA05041位于A05号染色体上第16988803位，其碱基为T或A；
    BrSNPA05042位于A05号染色体上第17247727位，其碱基为T或A；
    BrSNPA05043位于A05号染色体上第17810370位，其碱基为G或A；
    BrSNPA05044位于A05号染色体上第18626934位，其碱基为T或G；
    BrSNPA05045位于A05号染色体上第19009544位，其碱基为G或C；
    BrSNPA05046位于A05号染色体上第19022188位，其碱基为T或C；
    BrSNPA05047位于A05号染色体上第20978461位，其碱基为C或A；
    BrSNPA05048位于A05号染色体上第21254133位，其碱基为C或A；
    BrSNPA05049位于A05号染色体上第21498528位，其碱基为G或A；
    BrSNPA05050位于A05号染色体上第22030048位，其碱基为C或T；
    BrSNPA05051位于A05号染色体上第22731111位，其碱基为G或C；
    BrSNPA05052位于A05号染色体上第22895070位，其碱基为C或A；
    BrSNPA05053位于A05号染色体上第22962829位，其碱基为T或C；
    BrSNPA06001位于A06号染色体上第18887位，其碱基为C或G；
    BrSNPA06002位于A06号染色体上第117846位，其碱基为A或G；
    BrSNPA06003位于A06号染色体上第1296897位，其碱基为T或C；
    BrSNPA06004位于A06号染色体上第1752922位，其碱基为G或A；
    BrSNPA06005位于A06号染色体上第2426330位，其碱基为G或A；
    BrSNPA06006位于A06号染色体上第2789766位，其碱基为T或G；
    BrSNPA06007位于A06号染色体上第2931644位，其碱基为T或C；
    BrSNPA06008位于A06号染色体上第6382083位，其碱基为C或A；
    BrSNPA06009位于A06号染色体上第6403261位，其碱基为C或A；
    BrSNPA06010位于A06号染色体上第8245454位，其碱基为G或A；
    BrSNPA06011位于A06号染色体上第8618748位，其碱基为A或G；
    BrSNPA06012位于A06号染色体上第10107459位，其碱基为G或A；
    BrSNPA06013位于A06号染色体上第10568157位，其碱基为G或A；
    BrSNPA06014位于A06号染色体上第10578879位，其碱基为T或C；
    BrSNPA06015位于A06号染色体上第10874817位，其碱基为G或C；
    BrSNPA06016位于A06号染色体上第11069822位，其碱基为T或G；
    BrSNPA06017位于A06号染色体上第11344725位，其碱基为T或A；
    BrSNPA06018位于A06号染色体上第12784985位，其碱基为T或C；
    BrSNPA06019位于A06号染色体上第13424538位，其碱基为C或A；
    BrSNPA06020位于A06号染色体上第13447511位，其碱基为G或A；
    BrSNPA06021位于A06号染色体上第13534897位，其碱基为A或T；
    BrSNPA06022位于A06号染色体上第13545093位，其碱基为T或C；
    BrSNPA06023位于A06号染色体上第14701558位，其碱基为T或C；
    BrSNPA06024位于A06号染色体上第14726472位，其碱基为C或A；
    BrSNPA06025位于A06号染色体上第14730894位，其碱基为T或C；
    BrSNPA06026位于A06号染色体上第14731133位，其碱基为T或A；
    BrSNPA06027位于A06号染色体上第15606985位，其碱基为G或A；
    BrSNPA06028位于A06号染色体上第15685596位，其碱基为G或A；
    BrSNPA06029位于A06号染色体上第16018969位，其碱基为C或A；
    BrSNPA06030位于A06号染色体上第16193195位，其碱基为G或C；
    BrSNPA06031位于A06号染色体上第16201857位，其碱基为G或A；
    BrSNPA06032位于A06号染色体上第16271874位，其碱基为T或A；
    BrSNPA06033位于A06号染色体上第17483582位，其碱基为G或A；
    BrSNPA06034位于A06号染色体上第17610866位，其碱基为A或G；
    BrSNPA06035位于A06号染色体上第17773836位，其碱基为T或A；
    BrSNPA06036位于A06号染色体上第18376779位，其碱基为G或A；
    BrSNPA06037位于A06号染色体上第18546656位，其碱基为G或C；
    BrSNPA06038位于A06号染色体上第18584746位，其碱基为G或A；
    BrSNPA06039位于A06号染色体上第18685199位，其碱基为T或G；
    BrSNPA06040位于A06号染色体上第19361503位，其碱基为G或C；
    BrSNPA06041位于A06号染色体上第19387887位，其碱基为G或A；
    BrSNPA06042位于A06号染色体上第20048140位，其碱基为G或A；
    BrSNPA06043位于A06号染色体上第20833714位，其碱基为G或C；
    BrSNPA06044位于A06号染色体上第20977558位，其碱基为T或C；
    BrSNPA06045位于A06号染色体上第21415891位，其碱基为G或A；
    BrSNPA06046位于A06号染色体上第21632076位，其碱基为G或A；
    BrSNPA06047位于A06号染色体上第22374291位，其碱基为C或G；
    BrSNPA06048位于A06号染色体上第23153503位，其碱基为C或A；
    BrSNPA06049位于A06号染色体上第23563378位，其碱基为G或A；
    BrSNPA06050位于A06号染色体上第23928146位，其碱基为G或A；
    BrSNPA06051位于A06号染色体上第23955274位，其碱基为T或A；
    BrSNPA06052位于A06号染色体上第24041088位，其碱基为C或T；
    BrSNPA06053位于A06号染色体上第24284057位，其碱基为G或A；
    BrSNPA06054位于A06号染色体上第24717886位，其碱基为C或A；
    BrSNPA06055位于A06号染色体上第25147238位，其碱基为T或C；
    BrSNPA07001位于A07号染色体上第246275位，其碱基为G或C；
    BrSNPA07002位于A07号染色体上第641403位，其碱基为G或A；
    BrSNPA07003位于A07号染色体上第1028346位，其碱基为T或A；
    BrSNPA07004位于A07号染色体上第2560851位，其碱基为G或A；
    BrSNPA07005位于A07号染色体上第2970833位，其碱基为A或G；
    BrSNPA07006位于A07号染色体上第3216036位，其碱基为T或C；
    BrSNPA07007位于A07号染色体上第3279889位，其碱基为T或G；
    BrSNPA07008位于A07号染色体上第3358700位，其碱基为C或A；
    BrSNPA07009位于A07号染色体上第3961792位，其碱基为T或A；
    BrSNPA07010位于A07号染色体上第4234366位，其碱基为T或C；
    BrSNPA07011位于A07号染色体上第5021185位，其碱基为T或G；
    BrSNPA07012位于A07号染色体上第5153068位，其碱基为T或G；
    BrSNPA07013位于A07号染色体上第6101124位，其碱基为T或A；
    BrSNPA07014位于A07号染色体上第6254138位，其碱基为T或A；
    BrSNPA07015位于A07号染色体上第6429733位，其碱基为T或C；
    BrSNPA07016位于A07号染色体上第7129127位，其碱基为T或C；
    BrSNPA07017位于A07号染色体上第7137784位，其碱基为G或A；
    BrSNPA07018位于A07号染色体上第7249922位，其碱基为C或A；
    BrSNPA07019位于A07号染色体上第8738315位，其碱基为T或G；
    BrSNPA07020位于A07号染色体上第9057585位，其碱基为G或A；
    BrSNPA07021位于A07号染色体上第9463049位，其碱基为T或C；
    BrSNPA07022位于A07号染色体上第9678954位，其碱基为T或C；
    BrSNPA07023位于A07号染色体上第11143455位，其碱基为T或C；
    BrSNPA07024位于A07号染色体上第11295388位，其碱基为G或A；
    BrSNPA07025位于A07号染色体上第11498098位，其碱基为T或G；
    BrSNPA07026位于A07号染色体上第11520532位，其碱基为G或A；
    BrSNPA07027位于A07号染色体上第11551826位，其碱基为T或C；
    BrSNPA07028位于A07号染色体上第11750525位，其碱基为G或A；
    BrSNPA07029位于A07号染色体上第12234015位，其碱基为C或T；
    BrSNPA07030位于A07号染色体上第13554367位，其碱基为G或A；
    BrSNPA07031位于A07号染色体上第14074310位，其碱基为T或C；
    BrSNPA07032位于A07号染色体上第14523031位，其碱基为T或C；
    BrSNPA07033位于A07号染色体上第14747998位，其碱基为T或A；
    BrSNPA07034位于A07号染色体上第15655059位，其碱基为A或G；
    BrSNPA07035位于A07号染色体上第15740558位，其碱基为C或A；
    BrSNPA07036位于A07号染色体上第16130782位，其碱基为T或C；
    BrSNPA07037位于A07号染色体上第16491110位，其碱基为T或G；
    BrSNPA07038位于A07号染色体上第17285904位，其碱基为G或A；
    BrSNPA07039位于A07号染色体上第18060124位，其碱基为T或G；
    BrSNPA07040位于A07号染色体上第18453973位，其碱基为C或T；
    BrSNPA07041位于A07号染色体上第19178191位，其碱基为T或C；
    BrSNPA07042位于A07号染色体上第19761996位，其碱基为G或A；
    BrSNPA07043位于A07号染色体上第19894600位，其碱基为T或C；
    BrSNPA07044位于A07号染色体上第20112319位，其碱基为G或C；
    BrSNPA07045位于A07号染色体上第21087891位，其碱基为A或G；
    BrSNPA07046位于A07号染色体上第21941825位，其碱基为T或C；
    BrSNPA07047位于A07号染色体上第22261109位，其碱基为T或G；
    BrSNPA07048位于A07号染色体上第22291912位，其碱基为T或C；
    BrSNPA07049位于A07号染色体上第22522822位，其碱基为C或T；
    BrSNPA07050位于A07号染色体上第23348479位，其碱基为G或A；
    BrSNPA07051位于A07号染色体上第24460147位，其碱基为G或C；
    BrSNPA07052位于A07号染色体上第25407338位，其碱基为T或A；
    BrSNPA07053位于A07号染色体上第25633222位，其碱基为G或A；
    BrSNPA07054位于A07号染色体上第25744414位，其碱基为A或C；
    BrSNPA08001位于A08号染色体上第100717位，其碱基为C或G；
    BrSNPA08002位于A08号染色体上第240722位，其碱基为G或A；
    BrSNPA08003位于A08号染色体上第277090位，其碱基为T或C；
    BrSNPA08004位于A08号染色体上第926709位，其碱基为G或A；
    BrSNPA08005位于A08号染色体上第1715093位，其碱基为G或A；
    BrSNPA08006位于A08号染色体上第2121093位，其碱基为G或A；
    BrSNPA08007位于A08号染色体上第2374634位，其碱基为G或A；
    BrSNPA08008位于A08号染色体上第3289888位，其碱基为T或G；
    BrSNPA08009位于A08号染色体上第3422941位，其碱基为C或G；
    BrSNPA08010位于A08号染色体上第3911814位，其碱基为T或G；
    BrSNPA08011位于A08号染色体上第3915045位，其碱基为T或C；
    BrSNPA08012位于A08号染色体上第4419556位，其碱基为C或T；
    BrSNPA08013位于A08号染色体上第5430112位，其碱基为T或G；
    BrSNPA08014位于A08号染色体上第6266345位，其碱基为C或A；
    BrSNPA08015位于A08号染色体上第7150432位，其碱基为G或A；
    BrSNPA08016位于A08号染色体上第7588851位，其碱基为T或C；
    BrSNPA08017位于A08号染色体上第8135981位，其碱基为C或A；
    BrSNPA08018位于A08号染色体上第8933259位，其碱基为G或A；
    BrSNPA08019位于A08号染色体上第9415744位，其碱基为T或G；
    BrSNPA08020位于A08号染色体上第10869244位，其碱基为C或T；
    BrSNPA08021位于A08号染色体上第11195840位，其碱基为T或A；
    BrSNPA08022位于A08号染色体上第12382048位，其碱基为T或A；
    BrSNPA08023位于A08号染色体上第12394480位，其碱基为T或A；
    BrSNPA08024位于A08号染色体上第12855208位，其碱基为C或A；
    BrSNPA08025位于A08号染色体上第12939081位，其碱基为G或C；
    BrSNPA08026位于A08号染色体上第13838879位，其碱基为T或A；
    BrSNPA08027位于A08号染色体上第14102664位，其碱基为G或C；
    BrSNPA08028位于A08号染色体上第14112886位，其碱基为T或C；
    BrSNPA08029位于A08号染色体上第14137581位，其碱基为T或C；
    BrSNPA08030位于A08号染色体上第14580308位，其碱基为T或C；
    BrSNPA08031位于A08号染色体上第14960288位，其碱基为G或C；
    BrSNPA08032位于A08号染色体上第15007562位，其碱基为T或G；
    BrSNPA08033位于A08号染色体上第15287418位，其碱基为G或C；
    BrSNPA08034位于A08号染色体上第15734124位，其碱基为G或A；
    BrSNPA08035位于A08号染色体上第16162051位，其碱基为T或G；
    BrSNPA08036位于A08号染色体上第16415364位，其碱基为T或A；
    BrSNPA08037位于A08号染色体上第16583368位，其碱基为T或C；
    BrSNPA08038位于A08号染色体上第17179902位，其碱基为T或G；
    BrSNPA08039位于A08号染色体上第17537125位，其碱基为T或C；
    BrSNPA08040位于A08号染色体上第17877117位，其碱基为T或C；
    BrSNPA08041位于A08号染色体上第18085207位，其碱基为T或C；
    BrSNPA08042位于A08号染色体上第18668405位，其碱基为T或C；
    BrSNPA08043位于A08号染色体上第19658257位，其碱基为G或A；
    BrSNPA08044位于A08号染色体上第19860954位，其碱基为C或T；
    BrSNPA08045位于A08号染色体上第19861288位，其碱基为C或T；
    BrSNPA08046位于A08号染色体上第20202413位，其碱基为G或C；
    BrSNPA08047位于A08号染色体上第20416840位，其碱基为G或A；
    BrSNPA08048位于A08号染色体上第20458612位，其碱基为T或C；
    BrSNPA08049位于A08号染色体上第20716882位，其碱基为G或C；
    BrSNPA09001位于A09号染色体上第165179位，其碱基为G或C；
    BrSNPA09002位于A09号染色体上第211286位，其碱基为T或A；
    BrSNPA09003位于A09号染色体上第443160位，其碱基为T或A；
    BrSNPA09004位于A09号染色体上第583658位，其碱基为A或G；
    BrSNPA09005位于A09号染色体上第898133位，其碱基为T或A；
    BrSNPA09006位于A09号染色体上第1161182位，其碱基为T或C；
    BrSNPA09007位于A09号染色体上第1379762位，其碱基为G或A；
    BrSNPA09008位于A09号染色体上第1595348位，其碱基为T或G；
    BrSNPA09009位于A09号染色体上第1627618位，其碱基为G或C；
    BrSNPA09010位于A09号染色体上第1901646位，其碱基为T或A；
    BrSNPA09011位于A09号染色体上第1930677位，其碱基为G或C；
    BrSNPA09012位于A09号染色体上第3019969位，其碱基为G或T；
    BrSNPA09013位于A09号染色体上第3817906位，其碱基为G或C；
    BrSNPA09014位于A09号染色体上第3896403位，其碱基为G或A；
    BrSNPA09015位于A09号染色体上第3903092位，其碱基为G或C；
    BrSNPA09016位于A09号染色体上第4226515位，其碱基为G或A；
    BrSNPA09017位于A09号染色体上第4354836位，其碱基为C或A；
    BrSNPA09018位于A09号染色体上第4534869位，其碱基为C或A；
    BrSNPA09019位于A09号染色体上第4566148位，其碱基为G或A；
    BrSNPA09020位于A09号染色体上第4597771位，其碱基为G或C；
    BrSNPA09021位于A09号染色体上第5122605位，其碱基为C或A；
    BrSNPA09022位于A09号染色体上第5480210位，其碱基为T或C；
    BrSNPA09023位于A09号染色体上第5575779位，其碱基为T或G；
    BrSNPA09024位于A09号染色体上第6031641位，其碱基为T或C；
    BrSNPA09025位于A09号染色体上第6984636位，其碱基为T或C；
    BrSNPA09026位于A09号染色体上第7624272位，其碱基为C或T；
    BrSNPA09027位于A09号染色体上第7624862位，其碱基为G或T；
    BrSNPA09028位于A09号染色体上第7897532位，其碱基为T或C；
    BrSNPA09029位于A09号染色体上第8589991位，其碱基为G或T；
    BrSNPA09030位于A09号染色体上第8773906位，其碱基为G或T；
    BrSNPA09031位于A09号染色体上第8876252位，其碱基为T或A；
    BrSNPA09032位于A09号染色体上第8877380位，其碱基为C或G；
    BrSNPA09033位于A09号染色体上第9006311位，其碱基为C或A；
    BrSNPA09034位于A09号染色体上第9062940位，其碱基为T或C；
    BrSNPA09035位于A09号染色体上第9303625位，其碱基为T或G；
    BrSNPA09036位于A09号染色体上第9805886位，其碱基为C或T；
    BrSNPA09037位于A09号染色体上第10247545位，其碱基为C或A；
    BrSNPA09038位于A09号染色体上第11056315位，其碱基为T或A；
    BrSNPA09039位于A09号染色体上第11230572位，其碱基为G或A；
    BrSNPA09040位于A09号染色体上第12895394位，其碱基为G或A；
    BrSNPA09041位于A09号染色体上第13470942位，其碱基为T或C；
    BrSNPA09042位于A09号染色体上第16265081位，其碱基为A或C；
    BrSNPA09043位于A09号染色体上第17752348位，其碱基为G或C；
    BrSNPA09044位于A09号染色体上第18332606位，其碱基为T或C；
    BrSNPA09045位于A09号染色体上第18356344位，其碱基为G或A；
    BrSNPA09046位于A09号染色体上第18406676位，其碱基为T或C；
    BrSNPA09047位于A09号染色体上第18417691位，其碱基为T或G；
    BrSNPA09048位于A09号染色体上第20994890位，其碱基为T或C；
    BrSNPA09049位于A09号染色体上第21636788位，其碱基为G或A；
    BrSNPA09050位于A09号染色体上第21654909位，其碱基为T或C；
    BrSNPA09051位于A09号染色体上第21654935位，其碱基为T或C；
    BrSNPA09052位于A09号染色体上第21910417位，其碱基为T或C；
    BrSNPA09053位于A09号染色体上第22627850位，其碱基为G或A；
    BrSNPA09054位于A09号染色体上第23220955位，其碱基为G或A；
    BrSNPA09055位于A09号染色体上第23322031位，其碱基为G或A；
    BrSNPA09056位于A09号染色体上第25785187位，其碱基为G或A；
    BrSNPA09057位于A09号染色体上第26222141位，其碱基为T或C；
    BrSNPA09058位于A09号染色体上第26503872位，其碱基为T或A；
    BrSNPA09059位于A09号染色体上第26520788位，其碱基为G或C；
    BrSNPA09060位于A09号染色体上第26564841位，其碱基为G或A；
    BrSNPA09061位于A09号染色体上第26594548位，其碱基为T或A；
    BrSNPA09062位于A09号染色体上第26739505位，其碱基为T或A；
    BrSNPA09063位于A09号染色体上第26924828位，其碱基为G或C；
    BrSNPA09064位于A09号染色体上第27270361位，其碱基为T或C；
    BrSNPA09065位于A09号染色体上第28770187位，其碱基为T或C；
    BrSNPA09066位于A09号染色体上第29198476位，其碱基为T或C；
    BrSNPA09067位于A09号染色体上第30179678位，其碱基为G或A；
    BrSNPA09068位于A09号染色体上第30448774位，其碱基为T或A；
    BrSNPA09069位于A09号染色体上第30507943位，其碱基为T或C；
    BrSNPA09070位于A09号染色体上第31873135位，其碱基为T或C；
    BrSNPA09071位于A09号染色体上第31875876位，其碱基为T或C；
    BrSNPA09072位于A09号染色体上第32719234位，其碱基为C或T；
    BrSNPA09073位于A09号染色体上第32983605位，其碱基为G或A；
    BrSNPA09074位于A09号染色体上第33495110位，其碱基为A或C；
    BrSNPA09075位于A09号染色体上第34559009位，其碱基为G或A；
    BrSNPA09076位于A09号染色体上第37047265位，其碱基为T或G；
    BrSNPA09077位于A09号染色体上第37238042位，其碱基为C或A；
    BrSNPA09078位于A09号染色体上第37689520位，其碱基为T或A；
    BrSNPA09079位于A09号染色体上第37741739位，其碱基为G或A；
    BrSNPA09080位于A09号染色体上第37743859位，其碱基为G或A；
    BrSNPA10001位于A10号染色体上第9384位，其碱基为T或G；
    BrSNPA10002位于A10号染色体上第119709位，其碱基为T或A；
    BrSNPA10003位于A10号染色体上第617354位，其碱基为T或A；
    BrSNPA10004位于A10号染色体上第1861569位，其碱基为T或C；
    BrSNPA10005位于A10号染色体上第3689550位，其碱基为T或C；
    BrSNPA10006位于A10号染色体上第3708065位，其碱基为G或A；
    BrSNPA10007位于A10号染色体上第3721991位，其碱基为T或C；
    BrSNPA10008位于A10号染色体上第3800101位，其碱基为G或A；
    BrSNPA10009位于A10号染色体上第3834274位，其碱基为C或A；
    BrSNPA10010位于A10号染色体上第3849310位，其碱基为C或A；
    BrSNPA10011位于A10号染色体上第3878034位，其碱基为T或A；
    BrSNPA10012位于A10号染色体上第4497856位，其碱基为T或A；
    BrSNPA10013位于A10号染色体上第4782602位，其碱基为T或C；
    BrSNPA10014位于A10号染色体上第5601251位，其碱基为G或C；
    BrSNPA10015位于A10号染色体上第5671082位，其碱基为G或A；
    BrSNPA10016位于A10号染色体上第6046254位，其碱基为G或A；
    BrSNPA10017位于A10号染色体上第6294669位，其碱基为T或G；
    BrSNPA10018位于A10号染色体上第6364310位，其碱基为T或G；
    BrSNPA10019位于A10号染色体上第6477381位，其碱基为C或A；
    BrSNPA10020位于A10号染色体上第7174178位，其碱基为G或C；
    BrSNPA10021位于A10号染色体上第7989917位，其碱基为G或C；
    BrSNPA10022位于A10号染色体上第8425118位，其碱基为T或C；
    BrSNPA10023位于A10号染色体上第8648405位，其碱基为T或A；
    BrSNPA10024位于A10号染色体上第9700540位，其碱基为G或C；
    BrSNPA10025位于A10号染色体上第10373824位，其碱基为G或A；
    BrSNPA10026位于A10号染色体上第10740098位，其碱基为T或C；
    BrSNPA10027位于A10号染色体上第12134922位，其碱基为A或G；
    BrSNPA10028位于A10号染色体上第12492205位，其碱基为G或C；
    BrSNPA10029位于A10号染色体上第12527035位，其碱基为G或C；
    BrSNPA10030位于A10号染色体上第12619426位，其碱基为G或A；
    BrSNPA10031位于A10号染色体上第13105346位，其碱基为C或A；
    BrSNPA10032位于A10号染色体上第13127812位，其碱基为G或T；
    BrSNPA10033位于A10号染色体上第13406296位，其碱基为T或G；
    BrSNPA10034位于A10号染色体上第13716056位，其碱基为T或A；
    BrSNPA10035位于A10号染色体上第13732849位，其碱基为T或C；
    BrSNPA10036位于A10号染色体上第13859895位，其碱基为G或A；
    BrSNPA10037位于A10号染色体上第14319070位，其碱基为T或A；
    BrSNPA10038位于A10号染色体上第14443918位，其碱基为C或A；
    BrSNPA10039位于A10号染色体上第14465630位，其碱基为G或C；
    BrSNPA10040位于A10号染色体上第14638538位，其碱基为C或A；
    BrSNPA10041位于A10号染色体上第15240989位，其碱基为G或C；
    BrSNPA10042位于A10号染色体上第15835648位，其碱基为G或C；
    BrSNPA10043位于A10号染色体上第16198723位，其碱基为G或A。
  2. 根据权利要求1所述的应用，其特征在于：所述“用于检测所述571个SNP位点的物质”为用于检测所述571个SNP位点的571组引物，所述571组引物中的每组引物由分别记为上游引物1和上游引物2的两条上游引物和一条下游引物组成；所述上游引物1的结构组成自5’端至3’端依次为：通用序列1、基因组特异序列、所述SNP位点的两种核苷酸中的一种；所述上游引物2的结构组成自5’端至3’端依次为：通用序列2、基因组特异序列、所述SNP位点的两种核苷酸中的另一种；所述下游引物仅由基因组特异序列组成。
  3. 根据权利要求2所述的应用，其特征在于：在所述571组引物中，所有的所述上游引物中的基因组特异序列为将序列表中所有的序列X去除第1-21位后所 得的1142个序列；所有的所述下游引物的核苷酸序列为序列表中所有的序列Y；其中，X为3N-2或3N-1，Y为3N，N为大于等于1且小于等于571的整数。
  4. 根据权利要求3所述的应用，其特征在于：所述通用序列1的核苷酸序列如序列表中序列1的第1-21所示；所述通用序列2的核苷酸序列如序列表中序列2的第1-22所示。
  5. 根据权利要求4所述的应用，其特征在于：所述571组引物由序列表中序列1-序列1713所示的1713条单链DNA组成。
  6. 一种构建白菜DNA指纹数据库的方法，包括如下步骤：
    (a1)分别提取用于构建白菜DNA指纹数据库的所有白菜品种的基因组DNA；
    (a2)检测所述所有白菜品种的基因组DNA中权利要求1中所述的571个SNP位点的基因型，所得基因型数据即构成白菜DNA指纹数据库。
  7. 根据权利要求6所述的方法，其特征在于：所述方法的步骤(a2)中，是采用权利要求2-5任一中所述的“用于检测所述571个SNP位点的物质”检测所述所有白菜品种的基因组DNA中所述571个SNP位点的基因型的。
  8. 一种对白菜种质资源进行类群划分的方法，包括如下步骤：
    (b1)分别提取待进行类群划分的所有白菜品种的基因组DNA；
    (b2)检测所有所述白菜品种的基因组DNA中权利要求1中所述的571个SNP位点的基因型，根据所得基因型数据，建立聚类树状图，从而将所述所有白菜品种划分为不同类群。
  9. 根据权利要求8所述的方法，其特征在于：所述方法的步骤(b2)中，是采用权利要求2-5任一中所述的“用于检测所述571个SNP位点的物质”检测所述所有白菜品种的基因组DNA中所述571个SNP位点的基因型的。
  10. 一种白菜分子育种方法，包括如下步骤：将携带有目标性状的白菜供体亲本与白菜受体亲本杂交，再将所得杂交后代与所述白菜供体亲本进行若干次回交，分别检测所述白菜受体亲本以及所得的携带有所述目标性状的回交后代基因组DNA中权利要求1中所述的571个SNP位点的基因型，根据检测结果从携带有所述目标性状的所述回交后代中选择所述571个SNP位点的基因型与所述白菜受体亲本一致性最高的个体。
  11. 用于检测权利要求1中所述的571个SNP位点的基因型的产品，含有用于检测所述571个SNP位点的571组引物，所述571组引物中的每组引物由分别记为上游引物1和上游引物2的两条上游引物和一条下游引物组成；所述上游引物1的结构组成自5’端至3’端依次为：通用序列1、基因组特异序列、所述SNP位点的两种核苷酸中的一种；所述上游引物2的结构组成自5’端至3’端依次为：通用序列2、基因组特异序列、所述SNP位点的两种核苷酸中的另一种；所述下游引物仅由基因组特异序列组成。
  12. 根据权利要求11所述的产品，其特征在于：在所述571组引物中，所有 的所述上游引物中的基因组特异序列为将序列表中所有的序列X去除第1-21位后所得的1142个序列；所有的所述下游引物的核苷酸序列为序列表中所有的序列Y；其中，X为3N-2或3N-1，Y为3N，N为大于等于1且小于等于571的整数。
  13. 根据权利要求12所述的产品，其特征在于：所述通用序列1的核苷酸序列如序列表中序列1的第1-21所示；所述通用序列2的核苷酸序列如序列表中序列2的第1-22所示。
  14. 根据权利要求13所述的产品，其特征在于：所述571组引物由序列表中序列1-序列1713所示的1713条单链DNA组成。
  15. 权利要求11-14中任一所述的产品在检测或辅助检测白菜基因组DNA中权利要求1中所述571个SNP位点的基因型中的应用。
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