WO2016171344A1 - Saengjang, qui est une nouvelle variété d'aloès, et marqueur moléculaire de classification de celui-ci - Google Patents

Saengjang, qui est une nouvelle variété d'aloès, et marqueur moléculaire de classification de celui-ci Download PDF

Info

Publication number
WO2016171344A1
WO2016171344A1 PCT/KR2015/009884 KR2015009884W WO2016171344A1 WO 2016171344 A1 WO2016171344 A1 WO 2016171344A1 KR 2015009884 W KR2015009884 W KR 2015009884W WO 2016171344 A1 WO2016171344 A1 WO 2016171344A1
Authority
WO
WIPO (PCT)
Prior art keywords
aloe
seq
nos
oligonucleotide primer
primer sets
Prior art date
Application number
PCT/KR2015/009884
Other languages
English (en)
Korean (ko)
Inventor
김권희
고문경
장선일
황성수
고윤정
Original Assignee
김권희
고문경
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 김권희, 고문경 filed Critical 김권희
Publication of WO2016171344A1 publication Critical patent/WO2016171344A1/fr

Links

Images

Classifications

    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
    • A01H1/04—Processes of selection involving genotypic or phenotypic markers; Methods of using phenotypic markers for selection
    • A01H1/045—Processes of selection involving genotypic or phenotypic markers; Methods of using phenotypic markers for selection using molecular markers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids

Definitions

  • the present invention relates to a new breed of aloe (Saengjang) and a marker for determining the same.
  • Aloe is a succulent plant native to Africa.
  • the plant is native to South Africa, Madagascar, and Arabia, mainly in the African continent and neighboring islands, and is now widely cultivated in tropical and temperate regions.
  • the leaves are thick, sword-shaped, with thorns on the edges, and have orange or pink flowers.
  • It contains a large amount of ingredients useful for the human body, such as alloin and saponin, and has been developed and used in medicine, health supplements, and cosmetics since ancient times.
  • More than 600 species of Aloe are known, but currently edible aloe is on the order of Aloe vera (Aloe vera), aloe ahbore sense (Aloe arborescens), aloe sand paper or Syrian (Aloe saponaria).
  • Aloe vera is a representative species of aloe used for commercial and medicinal purposes. It is usually vulnerable only after three years of cultivation. However, because it is a tropical crop, domestic cultivation requires a relatively high cost, and imported products from foreign countries have a problem in that the quality is not constant due to uneven supply and demand imbalance. Aloe vera also removes toxic thick skin from the leaves and separates only the inner gel, which is a cumbersome process in the production of aloe-related products. Aloe saponaria, on the other hand, can be used as a peel because it is mild in aloe used as a raw leaf, but it belongs to a small species of plants of the genus Aloe.
  • Korean Patent No. 0133344 discloses a 'tissue culture method for mass propagation of aloe'
  • US Patent No. PP023913 discloses 'Aloe plant named Tiki Tahi', but the growth of new aloe species of the present invention and discrimination thereof are disclosed. No molecular markers have been disclosed.
  • the present invention is derived from the above requirements, by mixing aloe vera and saponaria planting natural hybrids with a thin shell and mass asexual breeding to cultivate new varieties and compare the morphological and molecular genetic characteristics Compared to aloe vera, the plant height is lower, the number of leaves is large, and there are white spots in the pale green leaves. Compared to the aloe vera, the leaf of the leaf is thinner and softer than the aloe vera.
  • the new aloe varieties named 'Saegjang' to complete the present invention.
  • the present invention has the following morphological features, has a white spots in the leaves of light green, compared to the aloe vera, the leaf is thinner and softer thorns, a new breed of aloe capable of mass propagation by asexual breeding Saengjang offers:
  • Bark thickness of the leaf 0.05-0.1 mm.
  • the present invention also provides oligonucleotide primer sets of SEQ ID NOs: 1 and 2; Oligonucleotide primer sets of SEQ ID NOs: 3 and 4; Oligonucleotide primer sets of SEQ ID NOs: 5 and 6; And one or more primer sets selected from the group consisting of oligonucleotide primer sets of SEQ ID NOs: 7 and 8.
  • the present invention comprises the steps of separating genomic DNA from aloe plant samples; Amplifying a target sequence by using the isolated genomic DNA as a template and performing an amplification reaction using the primer set of the present invention; And it provides a method for determining a new breed of aloe, comprising the step of detecting the amplification product.
  • Growth of a new aloe varieties according to the present invention is thin leaves and soft thorns can be used to grind the aloe leaves without removing the bark and drinking, 5-20 young shoots grow around the adult aloe to plant a new shoot The ability to breed in large quantities can greatly contribute to the increase in aloe farmers' income.
  • the primer set used in the present invention varieties for the determination of varieties according to the origin of aloe, purity test, breed protection and seed management system It can be used for testing.
  • 1 is a photograph showing the growth of the new year-old varieties (left) and Vera (right) as a control variety.
  • Figure 2 is a photograph showing the growth state of the new breed growth (left) and the control varieties Vera (right).
  • Figure 3 is a photograph showing a packaging foreground of new breed growth.
  • Figure 4 is a photograph showing the leaf cross section of aloe vera (left) and new varieties growth (right).
  • FIG. 5 is a photograph showing the flower form of aloe vera (left) and new breed growth (right).
  • FIG. 6 is a photograph showing aloe vera (A), aloe aborescens (B), aloe saponaria (C), and new breed growth (D).
  • FIG. 7 shows UPGMA phenograms showing genetic correlations between various aloe species, including aloe new species growth.
  • the present invention has the morphological characteristics of the following (a) to (g), has white spots in the leaves of light green, the leaf peel is thinner than the aloe vera, soft spines, asexual Providing a new breed of aloe Saengjang, which is capable of mass breeding by breeding:
  • Bark thickness of the leaf 0.05-0.1 mm.
  • New breed growth according to one embodiment of the present invention was cultivated in a mixed planting method of aloe vera and aloe saponaria to induce natural hybrids and obtain seedlings to grow lines through asexual breeding.
  • Aloe Vera (Aloe vera) belongs to the fastest growing section during 1-2 years after growing only deodiji two years, the flowers come up with a long spine, regardless of the season of the year smokes a yellow or reddish orange flowers.
  • the leaves are green, the leaves are 60 ⁇ 90cm in size, one leaf is about 0.5 ⁇ 1.5kg, and 3 ⁇ 6 leaves can be harvested when harvesting 5 ⁇ 6 year old one. Breeding is carried out by two or three young sprouts next to the mothers. When eaten, the bitter taste is strong, and when peeled and eaten, it's tasteless and odorless.
  • Aloe saponaria is a small species of aloe that grows on the ground and has light leaves with white spots. Flower beds rise long and bloom red-colored flowers. The leaf size is 30 ⁇ 60cm and the number of leaves of 1kg is 3 ⁇ 5. Aloe saponaria strikes 3-5 cubs. The bark has no bitter taste, so only the thorns are used and the bark is used a lot.
  • Aloe growth according to an embodiment of the present invention grows in the height of the body is 30 ⁇ 55cm, the stem is long and tender leaves grow in abandonment method, almost no growth after 1-2 years of growth, the leaves of growth 40cm, It has a light green background about 6cm wide with white spots, and leaves have small, weak spines.
  • aloe growth is characterized by the growth of more than 5-20 young seedlings near the root of the leaf body, the thickness of the leaf bark is thinner than the vera, and the flower stalk may not have branches.
  • New aloe varieties growth of the present invention was deposited on the microorganism resource center of Korea Research Institute of Bioscience and Biotechnology and deposited on callus KCTC 12780BP on March 30, 2015.
  • Tissue culture can regenerate a plant characterized by the present invention.
  • the regenerative cells used for such tissue culture may be immature embryos, plasma, meristem cells, callus, pollen, leaves, pollen sacs, roots, root tips or flowers, preferably callus.
  • the present invention also provides oligonucleotide primer sets of SEQ ID NOs: 1 and 2; Oligonucleotide primer sets of SEQ ID NOs: 3 and 4; Oligonucleotide primer sets of SEQ ID NOs: 5 and 6; And one or more primer sets selected from the group consisting of oligonucleotide primer sets of SEQ ID NOs: 7 and 8.
  • the oligonucleotide is an oligonucleotide consisting of at least 17, at least 18, at least 19, at least 20, at least 21 consecutive nucleotides in the sequence of SEQ ID NO: 1 and at least 17, 18 in the sequence of SEQ ID NO: 2. At least 19 and at least 20 contiguous nucleotide segments.
  • the oligonucleotide may be an oligonucleotide consisting of fragments of at least 17, at least 18, at least 19, at least 20 consecutive nucleotides in the sequences of SEQ ID NO: 3 and SEQ ID NO: 4, respectively.
  • the oligonucleotide is at least 17, at least 18, at least 19, at least 20, at least 21, at least 22, at least 23, at least 24, at least 25, and at least 26 in the sequence of SEQ ID NO.
  • the oligonucleotide is an oligonucleotide consisting of at least 17, at least 18, at least 19, at least 20 consecutive nucleotide segments in the sequence of SEQ ID NO: 7 and at least 17, at least 18, in the sequence of SEQ ID NO: 8, Oligonucleotides consisting of segments of at least 19, at least 20, at least 21, at least 22 consecutive nucleotides.
  • Oligonucleotide primer set is a combination of two primer sets, three primer set combinations, four primer set combinations of the four primer sets, most preferably four primer set combinations .
  • the oligonucleotide primer sets of the present invention are most preferably oligonucleotide primer sets of SEQ ID NOs: 1 and 2; Oligonucleotide primer sets of SEQ ID NOs: 3 and 4; Oligonucleotide primer sets of SEQ ID NOs: 5 and 6; It is a set of molecular label primers for the determination of new growth of aloe comprising the oligonucleotide primer set of SEQ ID NO: 7 and 8.
  • primer refers to a single stranded oligonucleotide sequence that is complementary to the nucleic acid strand to be copied and may serve as a starting point for the synthesis of the primer extension product.
  • the length and sequence of the primers should allow to start the synthesis of extension products. The specific length and sequence of the primer will depend on the primer utilization conditions such as temperature and ionic strength as well as the complexity of the DNA or RNA target required.
  • the plant's chloroplast genome (cpDNA) is easier to analyze than the nuclear genome because there are hundreds of identical chloroplast genomes in a single cell. It is a marker that distinguishes a specific species or a specific strain within the same species, and thus is the most reliable and quick analysis marker.
  • cpDNA chloroplast genome
  • An "Internal Transcribed Spacer” is a site that is transcribed from nuclear ribosomal DNA but not expressed as a protein.
  • ITS sequences have been used to infer subclass levels of a diverse array of organisms, including plants, fungi, insects, and the like.
  • the ITS region is known not only to have high information at the subclass level, but also to exhibit conserved sequence patterns and high alignment of pizza plants.
  • the ITS region is easily amplified in most pizza plants because small primers exhibit a peripheral sequence that is strongly conserved from the rRNA gene.
  • the base polymorphism was investigated in the ITS region amplified by SEQ ID NO: 7 and 8.
  • the invention also comprises the steps of separating genomic DNA from aloe plant samples; Amplifying a target sequence by using the isolated genomic DNA as a template and performing an amplification reaction using the primer set of the present invention; And it provides a method for determining a new breed of aloe, comprising the step of detecting the amplification product.
  • the method includes separating genomic DNA from aloe plant samples.
  • a method for separating genomic DNA from the sample a method known in the art may be used.
  • the CTAB method may be used, or a wizard prep kit (Promega) may be used.
  • oligonucleotide primer sets may be used as a primer to amplify a target sequence.
  • Methods for amplifying target nucleic acids include polymerase chain reaction (PCR), ligase chain reaction, nucleic acid sequence-based amplification, transcription-based amplification systems, There are any suitable methods for amplifying via strand displacement amplification or Q ⁇ replication or for amplifying nucleic acid molecules known in the art.
  • PCR is a method of amplifying a target nucleic acid from a pair of primers specifically binding to the target nucleic acid using a polymerase. Such PCR methods are well known in the art, and commercially available kits may be used.
  • the amplified target sequence may be labeled with a detectable labeling substance.
  • the labeling material may be, but is not limited to, a material emitting fluorescence, phosphorescence, or radioactivity.
  • the labeling substance is Cy-5 or Cy-3.
  • PCR is performed by labeling Cy-5 or Cy-3 at the 5'-end of the primer to allow the target sequence to be labeled with a detectable fluorescent labeling substance.
  • the label using the radioactive material may add radioactive isotopes such as 32 P or 35 S to the PCR reaction solution when PCR is performed, and the amplification product may be incorporated into the amplification product while the amplification product is synthesized.
  • the primer set used to amplify the target sequence is as described above.
  • the method of the present invention includes detecting the amplification product. Detection of the amplification product may be performed through sequencing, capillary electrophoresis, DNA chip, gel electrophoresis, radioactivity measurement, fluorescence measurement or phosphorescence measurement, but is not limited thereto. As one of methods for detecting amplification products, gel electrophoresis may be performed, and gel electrophoresis may use acrylamide gel electrophoresis or agarose gel electrophoresis depending on the size of the amplification product. Capillary electrophoresis can also be performed. Capillary electrophoresis can use, for example, an ABi Sequencer.
  • PCR is performed by labeling Cy-5 or Cy-3 at the 5'-end of a primer, and a target sequence is labeled with a detectable fluorescent labeling substance, and the labeled fluorescence is measured using a fluorimeter.
  • radioactivity measuring method is to add a radioactive isotope such as 32 P or 35 S to the PCR reaction solution to label the amplification product when performing PCR, and then radioactive measuring apparatus, for example, Geiger counter or liquid flash The radioactivity can be measured using a liquid scintillation counter.
  • aloe vera and aloe saponaria were artificially created in the greenhouse located in Yongjin-myeon, Wanju-gun, Jeonbuk, to artificially create environmental conditions for neglected moisture. .
  • seedlings with light green leaves with thin skins and thorny white spots were obtained in a greenhouse mixed with aloe vera and aloe saponaria, and since 1981, the lineage has been cultivated and the characteristics of the selection hybrids are maintained.
  • aloes are planted in a 600m 2 greenhouse located in Yongjin-myeon, Wanju-gun, Jeollabuk-do to avoid direct sunlight, and cultivate them at a cultivation rate of 20 ⁇ 35 °C in spring and autumn, and 10 ⁇ 15 °C in winter in banyang. Proliferated.
  • the ground part of the new breed growth of the present invention expressed the external characteristics of the vera and saponaria. Growth of new varieties was compared to morphological characteristics of Vera, the most similar variety in appearance.
  • the leaves of growth had white spots on pale green background about 40cm long and 6cm wide and small, weak spines at the edges of the leaves.
  • aloe vera showed a strong spine, dark green color, and no leaf pattern (Table 1, FIGS. 1 and 2).
  • the thickness of the leaf peel was thinner than the growth of Vera (Fig. 4).
  • the aloe vera showed a form in which the flower stalks branched, but in the case of growth, the flower stalks did not branch.
  • FIG. 6 Three Korean aloes, A. vera , Aloe arborescens , Aloe saponaria and Aloe new varieties were used as genomic DNA extraction samples (FIG. 6). 100 mg of leaves of aloe were taken and pulverized with liquid nitrogen, and DNA was extracted using a modified CTAB method. The extracted DNA was loaded on a 0.8% agarose gel to confirm the DNA state.
  • Primer sequences and annealing temperatures used in the present invention are as shown in Table 2.
  • Three chloroplast DNA regions (matK, trnL-F, rbcL) and nuclear ITS DNA regions were amplified.
  • the PCR reaction solution was 20 ⁇ l of total reaction solution, with 25ng of template DNA, 1 ⁇ PCR buffer, 2.5mM dNTP, 1U Taq DNA polymerase, and 20 pmol primer set (see Table 2 below) respectively. It was.
  • GeneAmp PCR system 2700 thermal cycler was used for PCR reaction, PCR reaction conditions were 95 °C (3 minutes), [94 °C (30 seconds), the corresponding annealing temperature of Table 2 (30 seconds), 72 °C (1 minutes), 35 cycles], 72 ° C. (10 minutes).
  • the PCR reaction product was extracted from agarose gel and analyzed for sequencing by ABI prism 3700 sequencer.
  • aloe growth was most closely collected with A. vera . Aloe growth is not yet reported in any kind of aloe It was not morphologically and genetically consistent with species in the genus. The results of the collection analysis of the investigated aloe species were in agreement with previous studies.
  • the aloe growth tissue was cut and cultured callus was deposited on March 30, 2015 with the accession number KCTC 12780BP to the Korea Institute of Bioscience and Biotechnology.
  • Aloe callus induction was performed by the following method. Only the areas where the stems including the aloe seedling roots were concentrated were cut. The cut aloe sample was subjected to surface sterilization with shaking for 10 minutes in a 10% commercial Lax solution. After the surface sterilization, the aloe sample was washed three times with sterile water. The sample was removed in a sterile workbench using a sterile filter paper to remove the remaining water, and then cut into a size of about 5 mm using a scalpel to induce the callus by incubating the medium.
  • Callus induction medium was prepared in MS (Murashige & Skoog) medium with 3% sugar, 0.4% gelrite, 0.4 mg / L thiamine-HCl, 100 mg / L myoinositol, 1 mg / L 2, Medium with 4-D and 0.3 mg / L kinetin added was used as basal medium. After about 3 weeks of incubation, a white cell mass was formed from the aloe slices in culture in the medium to which 2,4-D was added. Then, callus was proliferated through passage in the same medium.

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Physics & Mathematics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Botany (AREA)
  • Analytical Chemistry (AREA)
  • Environmental Sciences (AREA)
  • Developmental Biology & Embryology (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Immunology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Mycology (AREA)

Abstract

La présente invention concerne le Saengjang, qui est une nouvelle variété d'aloès, et un procédé permettant de classifier celui-ci. L'écorce de la feuille de Saengjang, qui est une nouvelle variété d'aloès, est mince et les épines sont souples, ce qui permet de boire des feuilles d'aloès broyées non pelées sans enlever l'écorce. En outre, étant donné qu'un aloès adulte produit 5 à 20 jeunes bourgeons, la propagation en masse est permise par transplantation des bourgeons, ce qui contribue dans une large mesure à une augmentation des revenus pour les fermes cultivant l'aloès. En outre, selon la présente invention, il est possible de classer l'aloès en utilisant quatre types de groupes d'amorces de marquage moléculaire, et le marqueur utilisé dans la présente invention s'applique normalement à la classification des variétés en fonction de l'origine de l'aloès, des tests de pureté et des tests de variétés pour la protection des variétés et l'établissement d'un système de gestion des graines.
PCT/KR2015/009884 2015-04-23 2015-09-21 Saengjang, qui est une nouvelle variété d'aloès, et marqueur moléculaire de classification de celui-ci WO2016171344A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
KR1020150057334A KR101563351B1 (ko) 2015-04-23 2015-04-23 알로에 신품종 생장 및 이를 판별하기 위한 분자마커
KR10-2015-0057334 2015-04-23

Publications (1)

Publication Number Publication Date
WO2016171344A1 true WO2016171344A1 (fr) 2016-10-27

Family

ID=54428299

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/KR2015/009884 WO2016171344A1 (fr) 2015-04-23 2015-09-21 Saengjang, qui est une nouvelle variété d'aloès, et marqueur moléculaire de classification de celui-ci

Country Status (2)

Country Link
KR (1) KR101563351B1 (fr)
WO (1) WO2016171344A1 (fr)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR101804120B1 (ko) 2016-12-02 2017-12-04 김권희 알로에 베라, 알로에 사포나리아 및 알로에 생장의 엽록체 게놈 및 핵 리보솜 dna 서열의 완전 해독 기반 종 식별 마커와 프라이머 세트 및 이의 용도

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR102237543B1 (ko) * 2019-02-22 2021-04-07 (주)모아캠 알로에 속 식물 유래 dna의 제조방법, 및 알로에 속 식물 유래 dna를 유효성분으로 포함하는 항노화 및 항염증용 조성물

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR0133344B1 (ko) * 1994-06-13 1998-04-14 김정문 알로에의 대량 증식방법
US6001572A (en) * 1996-07-26 1999-12-14 Univera Pharmaceuticals, Inc. Method of identifying Aloe using PCR
USPP23913P2 (en) * 2011-11-28 2013-09-17 Paulus A. M. van der Meer Aloe plant named ‘Tiki Tahi’

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE602006015492D1 (de) 2005-09-26 2010-08-26 Thegreencell Inc Transgene aloe-pflanzen zur erzeugung von proteinen und entsprechende verfahren

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR0133344B1 (ko) * 1994-06-13 1998-04-14 김정문 알로에의 대량 증식방법
US6001572A (en) * 1996-07-26 1999-12-14 Univera Pharmaceuticals, Inc. Method of identifying Aloe using PCR
USPP23913P2 (en) * 2011-11-28 2013-09-17 Paulus A. M. van der Meer Aloe plant named ‘Tiki Tahi’

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
KRISHNAMOORTHY ET AL.: "Genetic Relationship of Aloe Vera 'Saengjang', a New Forma, Based on cpDNA and ITS Sequence Variation", KOREAN JOURNAL OF PLANT TAXONOMY, vol. 44, no. 4, 2014, pages 250 - 256, XP055324180 *
WHANG, SUNG SOO ET AL.: "Genetic Relationship of Three Species of Aloe and a New Forma Based on Multiple DNA Markers", 2014 INTERNATIONAL SYMPOSIUM AND ANNUAL MEETING, LIFELONG HEALTH AND WELLNESS FROM NUTRITION AND FOOD, 27 October 2011 (2011-10-27), Daejeon, Korea *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR101804120B1 (ko) 2016-12-02 2017-12-04 김권희 알로에 베라, 알로에 사포나리아 및 알로에 생장의 엽록체 게놈 및 핵 리보솜 dna 서열의 완전 해독 기반 종 식별 마커와 프라이머 세트 및 이의 용도

Also Published As

Publication number Publication date
KR101563351B1 (ko) 2015-10-26

Similar Documents

Publication Publication Date Title
Li et al. Genetic diversity and classification of Nelumbo germplasm of different origins by RAPD and ISSR analysis
CN113637794B (zh) 一种果桑新品种‘粤椹201’的ssr分子标记及其核心引物组、试剂盒和应用
WO2014077464A1 (fr) Variétés de capsicum annuum résistantes à l'anthracnose développées par hybridation interspécifique et jeu d'amorces pour les cribler
WO2018101781A1 (fr) Marqueur d'identification d'espèce et ensemble d'amorces basé sur le séquençage complet du génome chloroplastique et sur la séquence d'adn ribosomique nucléaire d'aloe vera, d'aloe saponaria et d'aloe saengjang et utilisation correspondante
CN108949925B (zh) 一种快速准确鉴定杂草稻与栽培稻的分子检测方法
Szilagyi et al. Evaluation of genetic diversity in common bean (Phaseolus vulgaris L.) using RAPD markers and morpho-agronomic traits
Luo et al. Distinguishing Sichuan walnut cultivars and examining their relationships with Juglans regia and J. sigillata by FISH, early-fruiting gene analysis, and SSR analysis
CN108456740B (zh) 一个水稻稻瘟病抗性位点‘Pi-jx’及其Indel标记引物和育种应用
WO2016171344A1 (fr) Saengjang, qui est une nouvelle variété d'aloès, et marqueur moléculaire de classification de celui-ci
KR20080105075A (ko) 식물로부터 ｄｎａ를 분리하는 비-파괴 절차
CN110468229B (zh) 水稻广谱高抗白叶枯病基因Xa45(t)的共分离分子标记Hxjy-1
CN107663548B (zh) “申阳”草莓的ssr分子指纹图谱的构建方法及应用
CN108004236B (zh) 玉米茎腐病抗病分子育种方法及其应用
KR101783347B1 (ko) 배의 화분 유무 판별용 caps 마커 및 이의 용도
CN113637791B (zh) 一种同时鉴定辣椒雄性不育三系杂交种恢复性和真实性的分子标记及其鉴定方法
Daskalova et al. Initial determination of polymorphism and in vitro conservation of some Ramonda serbica and Ramonda nathaliae populations from Albania, Macedonia and Bulgaria
CN112575101B (zh) 与西葫芦prsv-w病毒病抗性相关的分子标记及其应用
CN114592086A (zh) 与环斑病病毒prsv抗性相关的snp分子标记及应用
CN109811085B (zh) 一种鉴定秋播大白菜珍绿6号种子纯度的引物及应用
CN110373487B (zh) 一种与辣椒辣味性状相关的InDel标记及其应用
CN114032323A (zh) 一种与雪茄烟抗黑胫病基因紧密连锁的共显性ssr标记及其应用
Heinze et al. Application of random amplified polymorphic DNA (RAPD) to detect genetic variation in Norway spruce
KR102066593B1 (ko) 참나리 신품종 '오렌지 뷰티' 및 이를 판별하기 위한 분자마커
CN109943659B (zh) 一种鉴定津研快绿1号小白菜种子纯度的引物及应用
JP4575613B2 (ja) アルミニウム耐性因子に連鎖するｄｎａマーカー、及びその利用

Legal Events

Date Code Title Description
121 Ep: the epo has been informed by wipo that ep was designated in this application

Ref document number: 15890010

Country of ref document: EP

Kind code of ref document: A1

NENP Non-entry into the national phase

Ref country code: DE

32PN Ep: public notification in the ep bulletin as address of the adressee cannot be established

Free format text: NOTING OF LOSS OF RIGHTS PURSUANT TO RULE 112(1) EPC (EPO FORM 1205A DATED 01.03.2018)

122 Ep: pct application non-entry in european phase

Ref document number: 15890010

Country of ref document: EP

Kind code of ref document: A1