WO2016104487A1 - Procédé de production en masse de caroténoïdes - Google Patents

Procédé de production en masse de caroténoïdes Download PDF

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Publication number
WO2016104487A1
WO2016104487A1 PCT/JP2015/085796 JP2015085796W WO2016104487A1 WO 2016104487 A1 WO2016104487 A1 WO 2016104487A1 JP 2015085796 W JP2015085796 W JP 2015085796W WO 2016104487 A1 WO2016104487 A1 WO 2016104487A1
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WO
WIPO (PCT)
Prior art keywords
algae
carotenoids
carotenoid
species belonging
trevoxia
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PCT/JP2015/085796
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English (en)
Japanese (ja)
Inventor
河野　重行
誠和 山▲崎▼
修平 大田
毅 竹下
英明 宮下
Original Assignee
国立大学法人東京大学
国立大学法人京都大学
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Publication date
Application filed by 国立大学法人東京大学, 国立大学法人京都大学 filed Critical 国立大学法人東京大学
Priority to JP2016566380A priority Critical patent/JP6847389B2/ja
Publication of WO2016104487A1 publication Critical patent/WO2016104487A1/fr

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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
    • A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
    • A61K8/9706—Algae
    • A61K8/9722—Chlorophycota or Chlorophyta [green algae], e.g. Chlorella
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
    • A61K8/33—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing oxygen
    • A61K8/34—Alcohols
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
    • A61K8/33—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing oxygen
    • A61K8/35—Ketones, e.g. benzophenone
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q19/00—Preparations for care of the skin
    • C—CHEMISTRY; METALLURGY
    • C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
    • C09K—MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE
    • C09K15/00—Anti-oxidant compositions; Compositions inhibiting chemical change
    • C09K15/34—Anti-oxidant compositions; Compositions inhibiting chemical change containing plant or animal materials of unknown composition
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/12—Unicellular algae; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P23/00—Preparation of compounds containing a cyclohexene ring having an unsaturated side chain containing at least ten carbon atoms bound by conjugated double bonds, e.g. carotenes

Definitions

  • the species belonging to the order of Trevoxia algae or Yokomida which is obtained by a method further comprising a step of drying the algal bodies collected in the step.
  • the species belonging to the Trevoxia algae is Chlorellales, Prasiolales, or the species belonging to the Watanabe family, (13) to (15), Species belonging to the eye.
  • “Sphaeropleales” is a group to which Chlorophyceae belongs, and has chloroplasts in the cell, similar to Trevoxia algae, and is composed mainly of ⁇ -1, 4 glucan by photosynthesis. Is an algae that can be biosynthesized. In the present specification, algae belonging to the order of Yokomida are often collectively referred to as “Yokomida algae”.
  • Stress application process is a process of applying intense light stress to Trevoxia algae or Yokomiwa algae during culture. The purpose of this step is to activate the carotenoid synthesis pathway of Trevoxia algae or Yokomiwa algae by applying stress, and accumulate a large amount of carotenoids in the algae.
  • the Trevoxia algae used in the present invention corresponds to the Trevoxia algae. Although it may be a species belonging to any order or lineage group, it is preferably a species belonging to the order Chlorella, Pleurotus or Watanabea. Species belonging to the order Chlorella include those belonging to the family Chlorellaceae.
  • the Chlorella family here is a concept that includes the Chlorella-clade group, the Parachlorella-clade group, and the Prototheca-clade group (Takashi Nakata, 2012, supervised by Nobuyoshi Watanabe, Algae Handbook, pp33-37, NTS Inc).
  • Species belonging to the genus Didymogenes such as Didymogenes anomala can produce and accumulate neoxanthin in large quantities in addition to lutein and / or zeaxanthin.
  • algae belonging to the order Chlorella of the Trevoxia algae especially species belonging to the family Chlorellaceae, can also accumulate anthaxanthin.
  • Specific examples of accumulating large amounts of anterraxanthin and its derivatives include, but are not limited to, species belonging to the genus Chlorella such as Chlorella sorokiniana.
  • Trevoxia algae or Yokowa midoro algae can grow and proliferate, so it is desirable to continue culturing during the application of stress.
  • the mutant is an auxotrophic mutant such as a cysteine-requiring mutant
  • prepare a medium that satisfies the auxotrophy by adding 500 ng / mL to 100 ⁇ g / mL of cysteine to the TAP medium.
  • Recovery Step “Recovery step” is a step of recovering the alga bodies of Trevoxia algae or Yokowa algae from the culture solution after the stress application step (S001). The purpose of this step is to recover the algal bodies in the form of a slurry by removing the culture medium and coarse matters from the culture solution.
  • This step reduces the total weight of Trevoxia algae or Yokowa amidrogae that have accumulated a large amount of carotenoids in the cells, and makes handling easier. Moreover, preservability is also increased.
  • the organic solvent used may be a low-polar or non-polar water-immiscible medium, such as an aliphatic or alicyclic hydrocarbon having 6 to 10 carbon atoms such as n-hexane or n-heptane, or benzene.
  • An aromatic hydrocarbon having 6 to 10 carbon atoms is preferable, and hexane or n-heptane is more preferable.
  • Trevoxia algae or Yokowa midoro algae composed of algal bodies after the stress application step (S001) is 150 to 500 hours at a light intensity of 500-2500 ⁇ mol photons / m 2 / s against Trevoxia algae or Yokowa midoro algae in culture It can be obtained by applying intense light stress that irradiates light. Since the specific application method of intense light stress has been described in detail in the first aspect, the description thereof is omitted in this aspect.
  • the Trevoxia algae or Yokowa midoro algae after this step are obtained in a mixed state in the culture solution.
  • Natural colorant is a pigment component derived from a natural product, and is an additive used to add to foods, beverages, cosmetics, pharmaceuticals, etc. and adjust the color tone. Plants, filamentous fungi, bacteria, and insects are known as natural products that serve as raw materials for natural colorants.
  • the natural colorant of the present invention includes a pigment component that is an essential component, and a solvent and / or carrier that is a selective component. Hereinafter, each component will be specifically described.
  • Oils include vegetable oils such as wheat germ oil, apricot oil, olive oil, camellia oil, evening primrose oil, aloe vera oil, rapeseed oil, palm oil, cottonseed oil, soybean oil, corn oil, safflower oil, sesame oil, linseed oil, castor oil Can be mentioned. Ethanol etc. are mentioned as an organic solvent.
  • fillers examples include petrolatum, the sugar and / or calcium phosphate.
  • the dosage form of the natural colorant of the present invention is not particularly limited as long as it is a dosage form suitable for addition and / or mixing to foods and drinks (including feed), cosmetics, pharmaceuticals and the like.
  • Granules, powders, pastes, gels, liquids, suspensions, and the like are preferable as dosage forms because they can be easily added to objects.
  • the antioxidant of the present invention contains a pharmaceutically or food hygiene acceptable solvent as necessary.
  • the specific solvent that can be used in the antioxidant of the present invention may be the same as the solvent in the natural colorant of the third aspect.
  • the dosage form of the antioxidant of the present invention may be appropriately determined according to the intended use.
  • a dosage form suitable for additive mixing may be used.
  • granules, powders, pastes, solutions, suspensions, gels, and the like are preferable because they can be easily added to an object.
  • a dosage form suitable for administration is preferred.
  • Administration methods can be broadly classified into oral administration and parenteral administration, so that dosage forms suitable for each administration method may be used.
  • the antioxidant of the present invention is usually preferably used as an oral administration agent, but is not limited thereto.
  • the antioxidant of the present invention is low as long as there is no danger to the living body, it is used as an additive for food and drink, food for specified health use, cosmetics, or pharmaceuticals with high antioxidant action and safety. can do.
  • “food and beverage” is a term that collectively refers to beverages, foods, and feeds.
  • Beverages include, for example, water (including purified water, mineral water, deep sea water), tea drinks (including green tea, oolong tea, and tea), coffee, coffee drinks, milk (including raw milk and processed milk), milk drinks, Lactic acid bacteria beverages, juices, soft drinks (including carbonated beverages, fruit juice beverages, powdered beverages, sports drinks), jelly beverages, cocoa beverages, brewed sake (including beer, sake, yellow wine, wine, cider), distilled spirits (whiskey) , Brandy, vodka, rum, tequila, shochu, white liquor, gin) and mixed liquor (including liqueur, plum wine, vermouth).
  • Food includes general foods (especially processed foods) and foods for specified health use.
  • the food / beverage product of this aspect is added with the natural colorant of the third aspect or the antioxidant of the fourth aspect during or after the production.
  • the method for producing a food or drink according to this aspect includes other food materials, various nutrients, various vitamins, minerals, amino acids, various fats and oils, and various additives (for example, flavoring ingredients, sweeteners, An acidulant such as an organic acid, a surfactant, a pH adjuster, a stabilizer, an antioxidant, a dye, and a flavor) may be blended and manufactured according to a conventional method.
  • the foodstuff which concerns on this invention can also be manufactured by adding the natural colorant of a 3rd aspect, or the antioxidant of a 4th aspect to the food-drinks after manufacture.
  • the 6th aspect of this invention is cosmetics.
  • the cosmetic of the present invention comprises the natural colorant of the third aspect or the antioxidant of the fourth aspect. ADVANTAGE OF THE INVENTION According to this invention, the cosmetics which have a color taste and an antioxidant effect can be provided.
  • the algae to be cultivated are C. mirabilis, C. vulgaris, C. sorokiniana and C. viscose belonging to Trevoxia algae, P. kessleri NIES-2152, P. kessleri PK2 and P. beijerinckii, Lobos. L. lobophora belonging to the genus Phaeropsis, G. emersonii belonging to the genus Graeciella belonging to the order of Yokowa midlo, and M. zofingiensis belonging to the genus Muriera were used. It seed
  • Example 2 Production of carotenoids using yellow algae (1)> (the purpose) The amount of carotenoid in the yellow algae treated by the carotenoid mass production method of the present invention is verified.
  • Table 1 shows the amount of each carotenoid per 1 L of the culture solution calculated from Examples 2 to 4.
  • the calculation of carotenoids by the absorbance method was in accordance with the method described in Wellburn, AR (J. Plant Physiol., 1994, 144, 307-313). There was no significant difference in the tendency of the amount of carotenoids by either the HPLC method or the absorbance method.

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Veterinary Medicine (AREA)
  • Organic Chemistry (AREA)
  • Public Health (AREA)
  • Animal Behavior & Ethology (AREA)
  • Epidemiology (AREA)
  • Birds (AREA)
  • Microbiology (AREA)
  • Zoology (AREA)
  • Botany (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Emergency Medicine (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • Mycology (AREA)
  • General Engineering & Computer Science (AREA)
  • Medicinal Chemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Virology (AREA)
  • Biomedical Technology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Dermatology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Cell Biology (AREA)
  • Materials Engineering (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Cosmetics (AREA)
  • Coloring Foods And Improving Nutritive Qualities (AREA)
  • Medicinal Preparation (AREA)

Abstract

　L'invention concerne un procédé de production en masse stable de caroténoïdes, à l'aide de minuscules algues qui se cultivent facilement et prolifèrent rapidement. Le procédé permet de stocker, intracellulairement, d'importantes quantités de caroténoïdes, par culture d'espèces appartenant à l'ordre des Sphaéropléales ou à la classe des Trébouxiophycées, avec application simultanée d'un stress par exposition à une lumière ayant une intensité de 500 à 2500 μmol de photons/m2/s pendant 150 à 500 heures.
PCT/JP2015/085796 2014-12-22 2015-12-22 Procédé de production en masse de caroténoïdes WO2016104487A1 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP2016566380A JP6847389B2 (ja) 2014-12-22 2015-12-22 カロテノイドの大量生産方法

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JP2014-259462 2014-12-22
JP2014259462 2014-12-22

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WO2016104487A1 true WO2016104487A1 (fr) 2016-06-30

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Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2019102732A1 (fr) * 2017-11-21 2019-05-31 株式会社タベルモ Composition contenant des algues pour aliments et boissons et son procédé de production
KR20190124351A (ko) * 2018-04-05 2019-11-05 삼척시 민물김 추출물을 유효성분으로 포함하는 피부 미백용 조성물
JPWO2021187546A1 (fr) * 2020-03-18 2021-09-23
KR20220080709A (ko) * 2020-12-07 2022-06-14 고려대학교 산학협력단 빛 조사 및 농부산물을 이용한 다이노잔틴의 대량생산방법 및 상기 방법으로 제조된 다이노잔틴을 유효성분으로 포함하는 녹조 제거용 조성물

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH01187082A (ja) * 1988-01-22 1989-07-26 Kurorera Kogyo Kk アスタキサンチン高含有緑藻類の生産方法
JPH06209757A (ja) * 1992-06-12 1994-08-02 Univ Ben Gurion 微生物成長装置
JPH0889279A (ja) * 1994-09-22 1996-04-09 Chikyu Kankyo Sangyo Gijutsu Kenkyu Kiko 緑藻類によるルテインの生産方法
JP2012044923A (ja) * 2010-08-26 2012-03-08 Tokyo Electric Power Co Inc:The 緑藻イカダモ、該緑藻イカダモの培養工程を有する脂質の製造方法、及び該緑藻イカダモの乾燥藻体
WO2015151577A1 (fr) * 2014-04-03 2015-10-08 日本水産株式会社 Procédé de production d'astaxanthine

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH01187082A (ja) * 1988-01-22 1989-07-26 Kurorera Kogyo Kk アスタキサンチン高含有緑藻類の生産方法
JPH06209757A (ja) * 1992-06-12 1994-08-02 Univ Ben Gurion 微生物成長装置
JPH0889279A (ja) * 1994-09-22 1996-04-09 Chikyu Kankyo Sangyo Gijutsu Kenkyu Kiko 緑藻類によるルテインの生産方法
JP2012044923A (ja) * 2010-08-26 2012-03-08 Tokyo Electric Power Co Inc:The 緑藻イカダモ、該緑藻イカダモの培養工程を有する脂質の製造方法、及び該緑藻イカダモの乾燥藻体
WO2015151577A1 (fr) * 2014-04-03 2015-10-08 日本水産株式会社 Procédé de production d'astaxanthine

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
CORDERO, BALDO F ET AL.: "Enhancement of lutein production in Chlorella sorokiniana (chorophyta) by improvement of culture conditions and random mutagenesis", MARINE DRUGS, vol. 9, no. 9, 20 September 2011 (2011-09-20), pages 1607 - 1624 *
HIROYUKI KAMOGAWA ET AL.: "Carotenoid Contents in Chlorella pyrenoidosa and Concentration by Saponification", BULLETIN OF FISHERIES SCIENCES, HOKKAIDO UNIVERSITY, vol. 62, no. 3, 14 December 2012 (2012-12-14), pages 83 - 88 *

Cited By (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2019102732A1 (fr) * 2017-11-21 2019-05-31 株式会社タベルモ Composition contenant des algues pour aliments et boissons et son procédé de production
JP2019092413A (ja) * 2017-11-21 2019-06-20 株式会社タベルモ 飲食品用藻類含有組成物及びその製造方法
KR20190124351A (ko) * 2018-04-05 2019-11-05 삼척시 민물김 추출물을 유효성분으로 포함하는 피부 미백용 조성물
KR102054132B1 (ko) 2018-04-05 2019-12-12 삼척시 민물김 추출물을 유효성분으로 포함하는 피부 미백용 조성물
JPWO2021187546A1 (fr) * 2020-03-18 2021-09-23
WO2021187546A1 (fr) * 2020-03-18 2021-09-23 国立大学法人筑波大学 Agent anti-maladie psychiatrique
KR20220080709A (ko) * 2020-12-07 2022-06-14 고려대학교 산학협력단 빛 조사 및 농부산물을 이용한 다이노잔틴의 대량생산방법 및 상기 방법으로 제조된 다이노잔틴을 유효성분으로 포함하는 녹조 제거용 조성물
KR102633212B1 (ko) * 2020-12-07 2024-02-02 고려대학교 산학협력단 빛 조사 및 농부산물을 이용한 다이노잔틴의 대량생산방법 및 상기 방법으로 제조된 다이노잔틴을 유효성분으로 포함하는 녹조 제거용 조성물

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JP2021045127A (ja) 2021-03-25
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