WO2016101317A1 - Processing method for increasing solubility and bioavailability of fat-soluble active component - Google Patents
Processing method for increasing solubility and bioavailability of fat-soluble active component Download PDFInfo
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- WO2016101317A1 WO2016101317A1 PCT/CN2014/095850 CN2014095850W WO2016101317A1 WO 2016101317 A1 WO2016101317 A1 WO 2016101317A1 CN 2014095850 W CN2014095850 W CN 2014095850W WO 2016101317 A1 WO2016101317 A1 WO 2016101317A1
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- 239000002245 particle Substances 0.000 claims abstract description 30
- 229920002472 Starch Polymers 0.000 claims abstract description 25
- 239000008107 starch Substances 0.000 claims abstract description 25
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- 235000019698 starch Nutrition 0.000 claims abstract description 15
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- OENHQHLEOONYIE-JLTXGRSLSA-N β-Carotene Chemical compound CC=1CCCC(C)(C)C=1\C=C\C(\C)=C\C=C\C(\C)=C\C=C\C=C(/C)\C=C\C=C(/C)\C=C\C1=C(C)CCCC1(C)C OENHQHLEOONYIE-JLTXGRSLSA-N 0.000 claims description 3
- UPYKUZBSLRQECL-UKMVMLAPSA-N Lycopene Natural products CC(=C/C=C/C=C(C)/C=C/C=C(C)/C=C/C1C(=C)CCCC1(C)C)C=CC=C(/C)C=CC2C(=C)CCCC2(C)C UPYKUZBSLRQECL-UKMVMLAPSA-N 0.000 claims description 2
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- ACTIUHUUMQJHFO-UPTCCGCDSA-N coenzyme Q10 Chemical compound COC1=C(OC)C(=O)C(C\C=C(/C)CC\C=C(/C)CC\C=C(/C)CC\C=C(/C)CC\C=C(/C)CC\C=C(/C)CC\C=C(/C)CC\C=C(/C)CC\C=C(/C)CCC=C(C)C)=C(C)C1=O ACTIUHUUMQJHFO-UPTCCGCDSA-N 0.000 claims description 2
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Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Definitions
- the invention relates to a processing method for improving the solubility and bioavailability of a fat-soluble active component, and belongs to the technical field of modern nutritious food processing.
- the object of the present invention is to provide a processing method for improving the solubility and bioavailability of a fat-soluble active component, which has the advantages of simple process, strong operability and high safety, and can significantly increase the solubility and biological activity of the active component of the poorly soluble food.
- the degree of utilization can be applied to many fields such as food, medicine, and daily chemicals.
- the object of the present invention is achieved by the following technical scheme: preparing a nano-sized fat-soluble active component-starch particle composite by nano-suspension technology using water-soluble starch particles as a carrier, thereby improving water solubility of the fat-soluble active component And absorption and utilization. Specific steps are as follows:
- control power 200-600W, and is treated at 0-10 ° C for 2-20 min;
- the water-soluble starch particles have a molecular weight of 10 7 -10 8 g/mol, a molecular density of 7%-10%, an average chain length of DP 10-12, an average particle size of 30-100 nm, and a fat-soluble activity.
- the components include carotenoid compounds, flavonoid compounds and the like, such as ⁇ -carotene, resveratrol, coenzyme Q10, lutein, lycopene, curcumin, quercetin, capsaicin and the like.
- the average particle size of the fat-soluble active component-starch composite was measured by Zetasizer Nano ZS nano-particle size analyzer at 30-150 nm, and the solubility of the fat-soluble active component in aqueous solution was increased by 20-150 times, cell membrane permeability. Increase by more than 5 times.
- the invention fully utilizes the resource-rich starch and natural phytochemicals in China, designs an effective fat-soluble active component protection and transportation system, improves food quality, and improves sustained-release efficacy and targeting.
- the method of the invention is simple, easy to operate, controllable reaction conditions, relatively low cost, and adopts a clean green production process, and has no pollution to the environment.
- the present invention significantly increases the solubility of the natural fat-soluble active component, and the state of existence changes from a crystalline state to a dissolved state, and is finally loaded into a water-soluble transport system, which greatly enhances the absorption and metabolism of nutrients in the body. Thereby improving its bioavailability, achieving the true supply of nutrients, better meeting the health demands of consumers, improving people's health and living standards.
- the products prepared by the invention can be applied to many fields such as food, medicine and daily chemicals, and the market prospect is very promising, and the economic benefits are broad.
- the water-soluble starch particles (molecular weight 1.5 ⁇ 10 7 g/mol, molecular density 9.5%, average chain length DP 10.2, average particle size 80.4 nm) were dissolved in a sodium acetate buffer solution of pH 7.0 and 0.1 mol/L to prepare a mass. a uniform solution with a concentration of 5%; the ⁇ -carotene is dissolved in an ethanol solution to form a uniform solution having a mass concentration of 5 mg/mL; and the total volume of the mixed solution is 200 mL, and the weight ratio of the water-soluble starch particles to the fat-soluble active component is 200.
- the fat-soluble active component-starch particle composite having good solubility is obtained.
- the average particle size of the fat-soluble active component-starch composite was measured by Zetasizer Nano ZS nanoparticle size analyzer to be 100.5 nm, and the solubility of the fat-soluble active component in aqueous solution was increased from 18.5 ⁇ g/mL to 1452.1 ⁇ g/mL. , increased by about 78.5 times, and the membrane permeability coefficient increased from 0.43 ⁇ 10 -6 cm/s to 8.65 ⁇ 10 -6 cm/s in the Caco-2 cell model, which was increased by about 20.1 times.
- the water-soluble starch particles (molecular weight 1.0 ⁇ 10 8 g/mol, molecular density 7.1%, average chain length DP11.8, average particle size 98.6 nm) were dissolved in a sodium acetate buffer solution of pH 7.0 and 0.1 mol/L.
- the treatment that is, obtaining a fat-soluble active component-starch particle composite having good solubility.
- the particle size of the fat-soluble active component-starch particle composite was measured by a Zetasizer Nano ZS nano-particle size analyzer to be 134.6 nm, and the solubility of the lip-soluble active component in the aqueous solution was increased from 42.7 ⁇ g/mL to 1148.6 ⁇ g/mL. The increase was about 26.9 times, and the membrane permeability coefficient increased from 0.85 ⁇ 10 -6 cm/s to 5.01 ⁇ 10 -6 cm/s in the Caco-2 cell model, which was increased by about 5.9 times.
Abstract
A processing method for increasing the solubility and bioavailability of a fat-soluble active component, the processing method comprising the following steps: (1) dissolving water-soluble starch particles in a sodium acetate buffer to prepare a homogeneous solution; (2) dissolving a fat-soluble active component in an ethanol solution to prepare another homogeneous solution; (3) mixing the two homogeneous solutions and simultaneously performing uninterrupted agitating at 30-70˚C to conduct a constant temperature reaction for 5-120 min; (4) placing the solutions into an ultrasonic device, the control power being 200-600 W, and processing the same at 1-10˚C for 2-20 min; and (5) performing a centrifugal treatment and performing a vacuum drying treatment of an obtained supernatant, so as to obtain a starch particle complex of a fat-soluble active component having good solubility and bioavailability.
Description
本发明涉及一种提高脂溶性活性组分溶解度与生物利用度的加工方法，属于现代营养食品加工技术领域。The invention relates to a processing method for improving the solubility and bioavailability of a fat-soluble active component, and belongs to the technical field of modern nutritious food processing.
随着社会科技和经济的发展、生活方式的转化及环境的恶化，我国疾病谱发生变化，糖尿病、高血压、肥胖症等慢性病数量剧增，亚健康状态的人群也越来越庞大。与此同时，人们的健康保健意识也越来越强烈，医疗观念已由病后治疗型向预防保健型转变。通过健康的生活方式、借助膳食营养等手段预防慢性疾病，正越来越被人们重视。With the development of social science and technology and economy, the transformation of lifestyles and the deterioration of the environment, the spectrum of diseases in China has changed. The number of chronic diseases such as diabetes, high blood pressure and obesity has increased dramatically, and the population of sub-health status has become larger and larger. At the same time, people's awareness of health care has become stronger and stronger, and the concept of medical care has changed from post-treatment treatment to preventive health care. Prevention of chronic diseases through healthy lifestyles and dietary nutrition is becoming more and more important.
功能性食品由于其显著的生理功能已成为消费者食谱中预防或减少慢性疾病发生的重要途径。然而，许多天然活性组分存在熔点高、水溶性差、易氧化分解、不易被人体消化吸收等特性，因此设计有效的活性组分传递系统来提高生物活性物质的利用率正成为该领域的研究重点。目前，罗氏制药、帝斯曼、巴斯夫等国外主要天然营养素生产供应商都开发了天然营养素的微胶囊及乳液保护技术并形成了规模化的生产销售；对于国内天然营养素企业的产品，则多数作为初级配料使用。基于上述原因，本发明对一种提高脂溶性活性组分溶解度与生物利用度的加工方法进行了详细研究。Functional foods have become an important way to prevent or reduce the occurrence of chronic diseases in consumer diets due to their remarkable physiological functions. However, many natural active components have high melting point, poor water solubility, easy oxidative decomposition, and are not easily digested and absorbed by the human body. Therefore, designing an effective active component delivery system to improve the utilization rate of biologically active substances is becoming a research focus in this field. . At present, Roche Pharmaceuticals, DSM, BASF and other major natural nutrient production suppliers have developed microcapsules and emulsion protection technologies for natural nutrients and formed large-scale production and sales; for domestic natural nutrient enterprises, most of them are primary. Use ingredients. Based on the above reasons, the present invention has conducted a detailed study on a processing method for improving the solubility and bioavailability of a fat-soluble active component.
发明内容Summary of the invention
本发明的目的在于提供一种提高脂溶性活性组分溶解度与生物利用度的加工方法，该方法具有工艺简单、操作性强、安全性高，可显著增加难溶性食品活性组分的溶解度和生物利用度，其应用范围可涉及到食品、医药、日用化学品等多个领域。The object of the present invention is to provide a processing method for improving the solubility and bioavailability of a fat-soluble active component, which has the advantages of simple process, strong operability and high safety, and can significantly increase the solubility and biological activity of the active component of the poorly soluble food. The degree of utilization can be applied to many fields such as food, medicine, and daily chemicals.
本发明的目的通过下述技术方案来实现：以水溶性淀粉粒子为载体，通过纳米混悬技术来制备纳米级脂溶性活性组分-淀粉粒子复合物，从而提高脂溶性活性组分的水溶性及吸收利用功效。具体步骤如下：The object of the present invention is achieved by the following technical scheme: preparing a nano-sized fat-soluble active component-starch particle composite by nano-suspension technology using water-soluble starch particles as a carrier, thereby improving water solubility of the fat-soluble active component And absorption and utilization. Specific steps are as follows:
(1)将水溶性淀粉粒子溶于pH7.0、0.1mol/L的醋酸钠缓冲液，配成质量浓度为5％-30％的均一溶液；(1) dissolving the water-soluble starch particles in a sodium acetate buffer solution of pH 7.0 and 0.1 mol/L to form a uniform solution having a mass concentration of 5% to 30%;
(2)将脂溶性活性组分溶于乙醇溶液，配成质量浓度为5-300mg/mL的均一溶液；(2) dissolving the fat-soluble active component in an ethanol solution to form a uniform solution having a mass concentration of 5-300 mg/mL;
(3)按混合溶液总体积200mL、水溶性淀粉粒子与脂溶性活性组分重量配比2:1-200:1利用上述两种均一溶液来进行复配，同时在30-70℃不断搅拌恒温反应5-120min；(3) According to the total volume of the mixed solution 200mL, the weight ratio of the water-soluble starch particles and the fat-soluble active component 2:1-200:1 using the above two homogeneous solutions for compounding, while constantly stirring at 30-70 ° C constant temperature Reaction 5-120 min;
(4)置于超声波作用装置中，控制功率为200-600W，在0-10℃下处理2-20min；
(4) placed in an ultrasonic device, the control power is 200-600W, and is treated at 0-10 ° C for 2-20 min;
(5)离心处理，将所获得上清液进行真空干燥处理，即获得具有良好溶解度与生物利用度的脂溶性活性组分-淀粉粒子复合物。(5) Centrifugation treatment, and the obtained supernatant is subjected to vacuum drying treatment to obtain a fat-soluble active component-starch particle composite having good solubility and bioavailability.
为了更好地实现本发明专利，水溶性淀粉粒子的分子量107-108g/mol，分子密度7％-10％，平均链长DP 10-12，平均颗粒大小30-100nm；脂溶性活性组分包括类胡萝卜化合物、类黄酮化合物等，如β-胡萝卜素、白藜芦醇、辅酶Q10、叶黄素、番茄红素、姜黄素、槲皮素、辣椒素等。In order to better realize the patent of the present invention, the water-soluble starch particles have a molecular weight of 10 7 -10 8 g/mol, a molecular density of 7%-10%, an average chain length of DP 10-12, an average particle size of 30-100 nm, and a fat-soluble activity. The components include carotenoid compounds, flavonoid compounds and the like, such as β-carotene, resveratrol, coenzyme Q10, lutein, lycopene, curcumin, quercetin, capsaicin and the like.
采用Zetasizer Nano ZS纳米粒度分析仪测出脂溶性活性组分-淀粉粒子复合物的平均颗粒大小在30-150nm，同时脂溶性活性组分在水溶液中的溶解度提高了20-150倍，细胞膜渗透性提高5倍以上。The average particle size of the fat-soluble active component-starch composite was measured by Zetasizer Nano ZS nano-particle size analyzer at 30-150 nm, and the solubility of the fat-soluble active component in aqueous solution was increased by 20-150 times, cell membrane permeability. Increase by more than 5 times.
本发明有以下优点：The invention has the following advantages:
1)本发明充分利用我国资源丰富的淀粉和天然植物化学物，设计有效的脂溶性活性组分保护和运输体系，改善食品品质，提高缓释功效及靶向性。1) The invention fully utilizes the resource-rich starch and natural phytochemicals in China, designs an effective fat-soluble active component protection and transportation system, improves food quality, and improves sustained-release efficacy and targeting.
2)本发明步骤简便，易于操作，反应条件可控，成本相对较低，而且采用清洁绿色生产工艺，对环境基本无污染。2) The method of the invention is simple, easy to operate, controllable reaction conditions, relatively low cost, and adopts a clean green production process, and has no pollution to the environment.
3)本发明使天然脂溶性活性组分的溶解度显著提高，存在状态从结晶态转变为溶解态，最终被载入到水溶性的运输体系中，这些都将大大提高营养素的体内吸收、代谢，从而提高其生物利用率，达到营养素的真正补给，更好地满足广大消费者对产品的健康诉求，提高人们的健康水平和生活水平。3) The present invention significantly increases the solubility of the natural fat-soluble active component, and the state of existence changes from a crystalline state to a dissolved state, and is finally loaded into a water-soluble transport system, which greatly enhances the absorption and metabolism of nutrients in the body. Thereby improving its bioavailability, achieving the true supply of nutrients, better meeting the health demands of consumers, improving people's health and living standards.
4)本发明制备的产品可应用于食品、医药、日用化学品等多个领域，市场前景十分看好，经济效益广阔。4) The products prepared by the invention can be applied to many fields such as food, medicine and daily chemicals, and the market prospect is very promising, and the economic benefits are broad.
下面结合实例进一步阐明本发明的内容，但本发明所保护的内容不仅仅局限于下面的实例。The contents of the present invention will be further clarified below with reference to examples, but the contents protected by the present invention are not limited to the following examples.
实施例1Example 1
将水溶性淀粉粒子(分子量1.5×107g/mol、分子密度9.5％、平均链长DP 10.2、平均颗粒大小80.4nm)溶于pH7.0、0.1mol/L的醋酸钠缓冲液配成质量浓度为5％的均一溶液；将β-胡萝卜素溶于乙醇溶液配成质量浓度为5mg/mL的均一溶液；按混合溶液总体积200mL、水溶性淀粉粒子与脂溶性活性组分重量配比200:1来混合上述两种均一溶液，同时在70℃不断搅拌恒温反应5min；置于超声波作用装置中，控制功率为600W，在0℃下处理20min；离心处理，将所获得上清液进行真空干燥处理，即获得具有良好溶解度的脂溶性活性组分-淀
粉粒子复合物。采用Zetasizer Nano ZS纳米粒度分析仪测出于脂溶性活性组分-淀粉粒子复合物的平均颗粒大小100.5nm，同时脂溶性活性组分在水溶液中的溶解度从18.5μg/mL增加到1452.1μg/mL，提高了约78.5倍，在Caco-2细胞模型中膜渗透系数从0.43×10-6cm/s增加到8.65×10-6cm/s，提高了约20.1倍。The water-soluble starch particles (molecular weight 1.5×10 7 g/mol, molecular density 9.5%, average chain length DP 10.2, average particle size 80.4 nm) were dissolved in a sodium acetate buffer solution of pH 7.0 and 0.1 mol/L to prepare a mass. a uniform solution with a concentration of 5%; the β-carotene is dissolved in an ethanol solution to form a uniform solution having a mass concentration of 5 mg/mL; and the total volume of the mixed solution is 200 mL, and the weight ratio of the water-soluble starch particles to the fat-soluble active component is 200. :1 to mix the above two homogeneous solutions, while stirring at 70 ° C constant temperature for 5 min; placed in an ultrasonic device, the control power is 600 W, treated at 0 ° C for 20 min; centrifugation, the obtained supernatant is vacuumed The drying treatment, that is, the fat-soluble active component-starch particle composite having good solubility is obtained. The average particle size of the fat-soluble active component-starch composite was measured by Zetasizer Nano ZS nanoparticle size analyzer to be 100.5 nm, and the solubility of the fat-soluble active component in aqueous solution was increased from 18.5 μg/mL to 1452.1 μg/mL. , increased by about 78.5 times, and the membrane permeability coefficient increased from 0.43×10 -6 cm/s to 8.65×10 -6 cm/s in the Caco-2 cell model, which was increased by about 20.1 times.
实施例2Example 2
将水溶性淀粉粒子(分子量1.0×108g/mol、分子密度7.1％、平均链长DP11.8、平均颗粒大小98.6nm)溶于pH7.0、0.1mol/L的醋酸钠缓冲液配成质量浓度为30％的均一溶液；将辅酶Q10溶于乙醇溶液配成质量浓度为300mg/mL的均一溶液；按混合溶液总体积200mL、水溶性淀粉粒子与脂溶性活性组分重量配比2:1来混合上述两种均一溶液，同时在30℃不断搅拌恒温反应120min；置于超声波作用装置中，控制功率为200W，在10℃下处理2min；离心处理，将所获得上清液进行真空干燥处理，即获得具有良好溶解度的脂溶性活性组分-淀粉粒子复合物。采用Zetasizer Nano ZS纳米粒度分析仪测出于脂溶性活性组分-淀粉粒子复合物的颗粒大小134.6nm，同时脂溶性活性组分在水溶液中的溶解度从42.7μg/mL增加到1148.6μg/mL，提高了约26.9倍，在Caco-2细胞模型中膜渗透系数从0.85×10-6cm/s增加到5.01×10-6cm/s，提高了约5.9倍。The water-soluble starch particles (molecular weight 1.0×10 8 g/mol, molecular density 7.1%, average chain length DP11.8, average particle size 98.6 nm) were dissolved in a sodium acetate buffer solution of pH 7.0 and 0.1 mol/L. A homogeneous solution having a mass concentration of 30%; a cosolvent Q10 dissolved in an ethanol solution to form a homogeneous solution having a mass concentration of 300 mg/mL; and a total volume of the mixed solution of 200 mL, a weight ratio of the water-soluble starch particles to the fat-soluble active component 2: 1 to mix the above two homogeneous solutions, while stirring at 30 ° C constant temperature reaction for 120min; placed in an ultrasonic device, control power of 200W, treated at 10 ° C for 2min; centrifugation, the obtained supernatant was vacuum dried The treatment, that is, obtaining a fat-soluble active component-starch particle composite having good solubility. The particle size of the fat-soluble active component-starch particle composite was measured by a Zetasizer Nano ZS nano-particle size analyzer to be 134.6 nm, and the solubility of the lip-soluble active component in the aqueous solution was increased from 42.7 μg/mL to 1148.6 μg/mL. The increase was about 26.9 times, and the membrane permeability coefficient increased from 0.85×10 -6 cm/s to 5.01×10 -6 cm/s in the Caco-2 cell model, which was increased by about 5.9 times.
本文所描述的具体实施案例仅作为对本发明精神和部分实验做举例说明。本发明所属领域的技术人员可以对所描述的具体实施案例做出各种各样的修改或补充或采用类似的方式替代，但并不会偏离本发明的精神或者超越所附权利要求书所定义的范围。
The specific embodiments described herein are merely illustrative of the spirit and part of the invention. A person skilled in the art can make various modifications or additions to the specific embodiments described, or in a similar manner, without departing from the spirit of the invention or as defined by the appended claims. The scope.
Claims (6)
- 一种提高脂溶性活性组分溶解度与生物利用度的加工方法，其特征在于以水溶性淀粉粒子为载体，通过纳米混悬技术来制备纳米级脂溶性活性组分-淀粉粒子复合物，从而提高脂溶性活性组分的水溶性及吸收利用功效，依次包括如下步骤：The invention relates to a processing method for improving the solubility and bioavailability of a fat-soluble active component, which is characterized in that nano-suspension active component-starch particle composite is prepared by nano-suspension technology by using water-soluble starch particles as a carrier, thereby improving The water solubility and absorption and utilization effects of the fat-soluble active component include the following steps:(1)将水溶性淀粉粒子溶于pH7.0、0.1mol/L的醋酸钠缓冲液，配成质量浓度为5％-30％的均一溶液；(1) dissolving the water-soluble starch particles in a sodium acetate buffer solution of pH 7.0 and 0.1 mol/L to form a uniform solution having a mass concentration of 5% to 30%;(2)将脂溶性活性组分溶于乙醇溶液，配成质量浓度为5-300mg/mL的均一溶液；(2) dissolving the fat-soluble active component in an ethanol solution to form a uniform solution having a mass concentration of 5-300 mg/mL;(3)按混合溶液总体积200mL、水溶性淀粉粒子与脂溶性活性组分重量配比2:1-200:1利用上述两种均一溶液来进行复配，同时在30-70℃不断搅拌恒温反应5-120min；(3) According to the total volume of the mixed solution 200mL, the weight ratio of the water-soluble starch particles and the fat-soluble active component 2:1-200:1 using the above two homogeneous solutions for compounding, while constantly stirring at 30-70 ° C constant temperature Reaction 5-120 min;(4)置于超声波作用装置中，控制功率为200-600W，在0-10℃下处理2-20min；(4) placed in an ultrasonic device, the control power is 200-600W, and is treated at 0-10 ° C for 2-20 min;(5)离心处理，将所获得上清液进行真空干燥处理，即获得具有良好溶解度与生物利用度的脂溶性活性组分-淀粉粒子复合物。(5) Centrifugation treatment, and the obtained supernatant is subjected to vacuum drying treatment to obtain a fat-soluble active component-starch particle composite having good solubility and bioavailability.
- 根据权利要求1所述的提高脂溶性活性组分溶解度与生物利用度的加工方法，其特征在于：水溶性淀粉粒子的分子量107-108g/mol，分子密度7％-10％，平均链长DP 10-12，平均颗粒大小30-100nm。The method for improving the solubility and bioavailability of a fat-soluble active component according to claim 1, wherein the water-soluble starch particles have a molecular weight of 10 7 - 10 8 g/mol and a molecular density of 7% - 10%, an average The chain length is DP 10-12 and the average particle size is 30-100 nm.
- 根据权利要求1所述的提高脂溶性活性组分溶解度与生物利用度的加工方法，其特征在于：脂溶性活性组分包括类胡萝卜化合物、类黄酮化合物，如β-胡萝卜素、白藜芦醇、辅酶Q10、叶黄素、番茄红素、姜黄素、槲皮素、辣椒素。The method for improving the solubility and bioavailability of a fat-soluble active component according to claim 1, wherein the fat-soluble active component comprises a carotenoid compound, a flavonoid compound such as β-carotene and resveratrol. Coenzyme Q10, lutein, lycopene, curcumin, quercetin, capsaicin.
- 根据权利要求1所述的提高脂溶性活性组分溶解度与生物利用度的加工方法，其特征在于脂溶性活性组分-淀粉粒子复合物平均颗粒大小为30-150nm。The method of processing for improving the solubility and bioavailability of a fat-soluble active ingredient according to claim 1, wherein the fat-soluble active component-starch particle composite has an average particle size of from 30 to 150 nm.
- 根据权利要求1所述的提高脂溶性活性组分溶解度与生物利用度的加工方法，其特征在于脂溶性活性组分在水溶液中的溶解度提高了20-150倍。The method for improving the solubility and bioavailability of a fat-soluble active ingredient according to claim 1, wherein the solubility of the fat-soluble active ingredient in the aqueous solution is increased by 20 to 150 times.
- 根据权利要求1所述的提高脂溶性活性组分溶解度与生物利用度的加工方法，其特征在于脂溶性活性组分的细胞膜渗透性提高5倍以上。 The method for improving the solubility and bioavailability of a fat-soluble active component according to claim 1, wherein the cell membrane permeability of the fat-soluble active component is increased by more than 5 times.
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