CN108308615A - A kind of corn peptide is the preparation method of the lutein nanometer grain of carrier - Google Patents
A kind of corn peptide is the preparation method of the lutein nanometer grain of carrier Download PDFInfo
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- CN108308615A CN108308615A CN201810030481.9A CN201810030481A CN108308615A CN 108308615 A CN108308615 A CN 108308615A CN 201810030481 A CN201810030481 A CN 201810030481A CN 108308615 A CN108308615 A CN 108308615A
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- lutein
- corn
- corn peptide
- nanometer grain
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- KBPHJBAIARWVSC-RGZFRNHPSA-N lutein Chemical compound C([C@H](O)CC=1C)C(C)(C)C=1\C=C\C(\C)=C\C=C\C(\C)=C\C=C\C=C(/C)\C=C\C=C(/C)\C=C\[C@H]1C(C)=C[C@H](O)CC1(C)C KBPHJBAIARWVSC-RGZFRNHPSA-N 0.000 title claims abstract description 88
- 229960005375 lutein Drugs 0.000 title claims abstract description 84
- 239000001656 lutein Substances 0.000 title claims abstract description 84
- 235000012680 lutein Nutrition 0.000 title claims abstract description 83
- ORAKUVXRZWMARG-WZLJTJAWSA-N lutein Natural products CC(=C/C=C/C=C(C)/C=C/C=C(C)/C=C/C1=C(C)CCCC1(C)C)C=CC=C(/C)C=CC2C(=CC(O)CC2(C)C)C ORAKUVXRZWMARG-WZLJTJAWSA-N 0.000 title claims abstract description 83
- KBPHJBAIARWVSC-XQIHNALSSA-N trans-lutein Natural products CC(=C/C=C/C=C(C)/C=C/C=C(C)/C=C/C1=C(C)CC(O)CC1(C)C)C=CC=C(/C)C=CC2C(=CC(O)CC2(C)C)C KBPHJBAIARWVSC-XQIHNALSSA-N 0.000 title claims abstract description 83
- FJHBOVDFOQMZRV-XQIHNALSSA-N xanthophyll Natural products CC(=C/C=C/C=C(C)/C=C/C=C(C)/C=C/C1=C(C)CC(O)CC1(C)C)C=CC=C(/C)C=CC2C=C(C)C(O)CC2(C)C FJHBOVDFOQMZRV-XQIHNALSSA-N 0.000 title claims abstract description 83
- 240000008042 Zea mays Species 0.000 title claims abstract description 75
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 title claims abstract description 75
- 235000002017 Zea mays subsp mays Nutrition 0.000 title claims abstract description 75
- 235000005822 corn Nutrition 0.000 title claims abstract description 75
- 108090000765 processed proteins & peptides Proteins 0.000 title claims abstract description 61
- 238000002360 preparation method Methods 0.000 title claims abstract description 31
- 239000000843 powder Substances 0.000 claims abstract description 17
- 102000004169 proteins and genes Human genes 0.000 claims abstract description 16
- 108090000623 proteins and genes Proteins 0.000 claims abstract description 16
- 238000000034 method Methods 0.000 claims abstract description 12
- 238000000108 ultra-filtration Methods 0.000 claims abstract description 6
- 239000012528 membrane Substances 0.000 claims abstract description 5
- 238000012216 screening Methods 0.000 claims abstract description 5
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 30
- 239000000243 solution Substances 0.000 claims description 25
- 235000019441 ethanol Nutrition 0.000 claims description 13
- 239000002245 particle Substances 0.000 claims description 8
- 239000007853 buffer solution Substances 0.000 claims description 7
- 239000007788 liquid Substances 0.000 claims description 7
- 239000002105 nanoparticle Substances 0.000 claims description 7
- 239000006166 lysate Substances 0.000 claims description 6
- 108091005804 Peptidases Proteins 0.000 claims description 5
- 239000004365 Protease Substances 0.000 claims description 5
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 claims description 5
- 239000006185 dispersion Substances 0.000 claims description 5
- 238000003756 stirring Methods 0.000 claims description 5
- 102000004190 Enzymes Human genes 0.000 claims description 4
- 108090000790 Enzymes Proteins 0.000 claims description 4
- 239000003513 alkali Substances 0.000 claims description 4
- 238000004090 dissolution Methods 0.000 claims description 4
- 239000002994 raw material Substances 0.000 claims description 4
- 239000007921 spray Substances 0.000 claims description 4
- MVORZMQFXBLMHM-QWRGUYRKSA-N Gly-His-Lys Chemical compound NCCCC[C@@H](C(O)=O)NC(=O)[C@@H](NC(=O)CN)CC1=CN=CN1 MVORZMQFXBLMHM-QWRGUYRKSA-N 0.000 claims description 3
- 238000009835 boiling Methods 0.000 claims description 3
- 108010038983 glycyl-histidyl-lysine Proteins 0.000 claims description 3
- 238000002390 rotary evaporation Methods 0.000 claims description 3
- 230000001954 sterilising effect Effects 0.000 claims description 3
- 239000006228 supernatant Substances 0.000 claims description 3
- 230000009849 deactivation Effects 0.000 claims description 2
- 230000008569 process Effects 0.000 claims description 2
- 238000002203 pretreatment Methods 0.000 claims 1
- 230000000050 nutritive effect Effects 0.000 abstract description 5
- 238000001727 in vivo Methods 0.000 abstract description 4
- 230000008901 benefit Effects 0.000 abstract description 2
- 235000015816 nutrient absorption Nutrition 0.000 abstract 1
- 235000013339 cereals Nutrition 0.000 description 22
- 238000010521 absorption reaction Methods 0.000 description 9
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 7
- 235000013305 food Nutrition 0.000 description 6
- 210000001525 retina Anatomy 0.000 description 6
- 230000000694 effects Effects 0.000 description 4
- 230000006870 function Effects 0.000 description 4
- 206010064930 age-related macular degeneration Diseases 0.000 description 3
- 239000003963 antioxidant agent Substances 0.000 description 3
- 239000003814 drug Substances 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 3
- 230000036541 health Effects 0.000 description 3
- 208000002780 macular degeneration Diseases 0.000 description 3
- 239000000463 material Substances 0.000 description 3
- 239000002539 nanocarrier Substances 0.000 description 3
- 235000016709 nutrition Nutrition 0.000 description 3
- 210000001519 tissue Anatomy 0.000 description 3
- KKAJSJJFBSOMGS-UHFFFAOYSA-N 3,6-diamino-10-methylacridinium chloride Chemical compound [Cl-].C1=C(N)C=C2[N+](C)=C(C=C(N)C=C3)C3=CC2=C1 KKAJSJJFBSOMGS-UHFFFAOYSA-N 0.000 description 2
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 2
- 240000007594 Oryza sativa Species 0.000 description 2
- 235000007164 Oryza sativa Nutrition 0.000 description 2
- 108010055615 Zein Proteins 0.000 description 2
- 229920002494 Zein Polymers 0.000 description 2
- 230000003078 antioxidant effect Effects 0.000 description 2
- 235000006708 antioxidants Nutrition 0.000 description 2
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 2
- 235000021466 carotenoid Nutrition 0.000 description 2
- 150000001747 carotenoids Chemical class 0.000 description 2
- 230000006378 damage Effects 0.000 description 2
- 239000003995 emulsifying agent Substances 0.000 description 2
- 230000004438 eyesight Effects 0.000 description 2
- 238000010438 heat treatment Methods 0.000 description 2
- 239000010977 jade Substances 0.000 description 2
- 210000002189 macula lutea Anatomy 0.000 description 2
- 230000035764 nutrition Effects 0.000 description 2
- 239000003960 organic solvent Substances 0.000 description 2
- 229910052760 oxygen Inorganic materials 0.000 description 2
- 239000001301 oxygen Substances 0.000 description 2
- 102000004196 processed proteins & peptides Human genes 0.000 description 2
- 239000000047 product Substances 0.000 description 2
- 150000003254 radicals Chemical class 0.000 description 2
- 235000009566 rice Nutrition 0.000 description 2
- 238000000926 separation method Methods 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- QCVGEOXPDFCNHA-UHFFFAOYSA-N 5,5-dimethyl-2,4-dioxo-1,3-oxazolidine-3-carboxamide Chemical compound CC1(C)OC(=O)N(C(N)=O)C1=O QCVGEOXPDFCNHA-UHFFFAOYSA-N 0.000 description 1
- 206010006187 Breast cancer Diseases 0.000 description 1
- 208000026310 Breast neoplasm Diseases 0.000 description 1
- 201000009030 Carcinoma Diseases 0.000 description 1
- MYMOFIZGZYHOMD-UHFFFAOYSA-N Dioxygen Chemical compound O=O MYMOFIZGZYHOMD-UHFFFAOYSA-N 0.000 description 1
- 102000002322 Egg Proteins Human genes 0.000 description 1
- 108010000912 Egg Proteins Proteins 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 238000008214 LDL Cholesterol Methods 0.000 description 1
- 206010028980 Neoplasm Diseases 0.000 description 1
- 206010060862 Prostate cancer Diseases 0.000 description 1
- 208000000236 Prostatic Neoplasms Diseases 0.000 description 1
- 208000034189 Sclerosis Diseases 0.000 description 1
- 208000027418 Wounds and injury Diseases 0.000 description 1
- 238000009825 accumulation Methods 0.000 description 1
- 239000004480 active ingredient Substances 0.000 description 1
- 238000013019 agitation Methods 0.000 description 1
- 150000001298 alcohols Chemical class 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 230000001093 anti-cancer Effects 0.000 description 1
- 210000001367 artery Anatomy 0.000 description 1
- 230000036772 blood pressure Effects 0.000 description 1
- 239000000969 carrier Substances 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 238000007385 chemical modification Methods 0.000 description 1
- 239000002131 composite material Substances 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 239000011258 core-shell material Substances 0.000 description 1
- 239000002537 cosmetic Substances 0.000 description 1
- 239000013078 crystal Substances 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 230000007850 degeneration Effects 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 238000009826 distribution Methods 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 235000013399 edible fruits Nutrition 0.000 description 1
- 235000014103 egg white Nutrition 0.000 description 1
- 210000000969 egg white Anatomy 0.000 description 1
- 235000013601 eggs Nutrition 0.000 description 1
- 208000030533 eye disease Diseases 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 235000013373 food additive Nutrition 0.000 description 1
- 239000002778 food additive Substances 0.000 description 1
- 235000012055 fruits and vegetables Nutrition 0.000 description 1
- 230000002496 gastric effect Effects 0.000 description 1
- 210000004051 gastric juice Anatomy 0.000 description 1
- 210000001035 gastrointestinal tract Anatomy 0.000 description 1
- 230000001279 glycosylating effect Effects 0.000 description 1
- 229940095686 granule product Drugs 0.000 description 1
- 231100001261 hazardous Toxicity 0.000 description 1
- 230000002209 hydrophobic effect Effects 0.000 description 1
- 230000028993 immune response Effects 0.000 description 1
- 230000036039 immunity Effects 0.000 description 1
- 230000002757 inflammatory effect Effects 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 230000002401 inhibitory effect Effects 0.000 description 1
- 208000014674 injury Diseases 0.000 description 1
- 230000003993 interaction Effects 0.000 description 1
- 230000002427 irreversible effect Effects 0.000 description 1
- 239000006210 lotion Substances 0.000 description 1
- 230000014759 maintenance of location Effects 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 208000001491 myopia Diseases 0.000 description 1
- 230000004379 myopia Effects 0.000 description 1
- 230000003647 oxidation Effects 0.000 description 1
- 238000007254 oxidation reaction Methods 0.000 description 1
- 230000004792 oxidative damage Effects 0.000 description 1
- 230000001766 physiological effect Effects 0.000 description 1
- 230000035790 physiological processes and functions Effects 0.000 description 1
- 239000000049 pigment Substances 0.000 description 1
- 229920000136 polysorbate Polymers 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 230000001681 protective effect Effects 0.000 description 1
- 230000005855 radiation Effects 0.000 description 1
- 201000001275 rectum cancer Diseases 0.000 description 1
- 230000000979 retarding effect Effects 0.000 description 1
- 230000002000 scavenging effect Effects 0.000 description 1
- 239000002904 solvent Substances 0.000 description 1
- 238000005507 spraying Methods 0.000 description 1
- 239000003381 stabilizer Substances 0.000 description 1
- 238000003860 storage Methods 0.000 description 1
- 208000024891 symptom Diseases 0.000 description 1
- 230000002195 synergetic effect Effects 0.000 description 1
- 235000015112 vegetable and seed oil Nutrition 0.000 description 1
- 235000019871 vegetable fat Nutrition 0.000 description 1
- 239000008158 vegetable oil Substances 0.000 description 1
- 238000005303 weighing Methods 0.000 description 1
- 239000005019 zein Substances 0.000 description 1
- 229940093612 zein Drugs 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/105—Plant extracts, their artificial duplicates or their derivatives
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/17—Amino acids, peptides or proteins
- A23L33/18—Peptides; Protein hydrolysates
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23P—SHAPING OR WORKING OF FOODSTUFFS, NOT FULLY COVERED BY A SINGLE OTHER SUBCLASS
- A23P10/00—Shaping or working of foodstuffs characterised by the products
- A23P10/30—Encapsulation of particles, e.g. foodstuff additives
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/34—Extraction; Separation; Purification by filtration, ultrafiltration or reverse osmosis
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/06—Preparation of peptides or proteins produced by the hydrolysis of a peptide bond, e.g. hydrolysate products
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Abstract
A kind of corn peptide is the preparation method of the lutein nanometer grain of carrier, and it is method that carrier prepares lutein nanometer grain that the present invention relates to a kind of using corn peptide.The present invention solves the problems, such as that unstable lutein, poorly water-soluble, nutrient absorption utilization rate are low.The present invention carries out as follows：1. corn protein powder is carried out screening and pre-press；2. it is enzymolysis modified to corn protein powder progress, then use Ultra filtration membrane target corn protein peptide；3. loading lutein nanometer grain using high-pressure homogeneous preparation corn peptide.The present invention is the advantages of a kind of corn peptide is the lutein nanometer grain of carrier：One, lutein stability is good, and nutritive value is high；Two, granularity is small, dissolubility is high；Three, safe using edible albumen fret peptide；Four, technique is advanced, simple, has a extensive future.It is a kind of high nutritive value, stability and dissolubility is good and the high corn peptide of utilization rate loads the preparation method of lutein nanometer grain in vivo.
Description
Technical field
The present invention relates to a kind of preparation methods for the lutein nanometer grain that corn peptide is carrier.
Background technology
Lutein also known as " lutern ", belong to carotenoid, are distributed widely in the fruit, branches and leaves and flower of plant, people
Can absorb lutein from the food such as fruit and vegetable.
Lutein is carotenoid important in human body, is distributed widely in the multiple tissues of human body, to maintaining health to play weight
It acts on.Lutein is a kind of important antioxidant, has efficiently die out singlet oxygen and effect of scavenging radical, can protect
The damage that human body is brought from oxygen and Free Radical enhances immunity of organisms；Lutein can be by filtering blue light and resisting
Oxidation protects skin, retina and internal multiple tissues from oxidative damage；Lutein has the function of protecting eyesight, leaf
Flavine and luteole are two kinds of primary pigments in retina, and lutein has important protective effect to the macula lutea in retina,
Lutein can absorb blue light, and the wavelength of blue visible light and ultraviolet light are close, are potential in the visible light for can reach retina
A kind of maximum light of harmfulness, on reaching retina before sensitive cell, light passes through the highest accumulation regions of lutein, if at this time
It is abundant depending on the lutein content at macula lutea, injury can be minimized, content of the lutein in retina is with the age
Increase declines year by year, and when shortage easily causes macular degeneration and eye-blurred, and then vision degeneration occurs, the symptoms such as myopia, more
It easily causes such as age-related macular degeneration (age-related macular degenera-tion, AMD) this irreversible blinding
Property eye disease.Lutein can also inhibit inflammatory and the immune response of in-vivo tissue；Leaf Huang rope in arterial wall cell can also reduce
The oxidisability of LDL cholesterol has retarding action to the artery sclerosis process of early stage；Multiple studies have shown that lutein is to a variety of cancers
Disease such as breast cancer, prostate cancer, the carcinoma of the rectum, cutaneum carcinoma etc. has prevention and inhibiting effect.
But the chemical constitution of lutein determines its physicochemical characteristics：Since carbochain is longer in its molecular structure
And containing more hydrophobic grouping, dissolubility is poor in water, reduces body absorption and bioavailability；Containing multiple in its molecule
Unsaturated double-bond causes easily to be influenced by the factors such as oxygen, light radiation, high temperature, pH value and internal enzyme, antibody and hydrochloric acid in gastric juice and occur
It decomposes and inactivates, lose original physiological activity, stability is poor.The two factors seriously limit lutein entrance and arrive in vivo
It is absorbed up to active component and preferably, it is difficult to effectively play its physiological function.Simultaneously lutein is also greatly affected to exist
The application in food and medicine field.
Nanosizing is that a kind of physical modification means are compared with chemical modification, has and does not change original molecular structure and main
Chemical characteristic is that current raising drug and Active components of food are molten with higher stability and better reservation bioactivity
Solution property and the method for promoting body absorption more commonly used, can reduce the particle size of lutein by nanosizing, and table is compared in increase
Area and dispersion performance in water are conducive to gastrointestinal tract absorption.Therefore it is micro-nano to be usually used in food, medicine and cosmetic field
The preparation of embedding system.
It is prepared into nano particle conduct currently, mainly being embedded regarding to the issue above using a little carriers to the exploitation of lutein
Food additives and health products are edible, a kind of preparation of lutein nanometer liquid as disclosed in Chinese patent CN102329520A applications
Method：Total antioxidation agent and stabilizer are added in vegetable fat, dissolved by heating, then lutein crystal is added to vegetable oil
In, it is dispersed with stirring uniformly, through ball mill, superhigh-voltage homogenizing machine or high pressure microjet, is crushed to nanoscale, lutein nanometer is made
Liquid.Method and technology are simple, securely and reliably, maintain the nutritional activities of lutein.But it is yellow without suitable carrier protection leaf
Element, it is poor to photo-thermal equistability, and nanometer system belongs to oil-soluble, bad dispersibility, body absorption are ineffective in water.For another example
104095816 A of Chinese patent CN disclose lutein ester nano particle and preparation method thereof：It first in a heated condition will package
Material is dissolved in a kind of organic solvent, after then mixing lutein ester with same organic solvent, is dissolved by heating, mechanical agitation
It is lower that lutein ester solution batch is added in lapping solution and is added emulsifier, so that solution particles is reached nanoscale, then
The solvent in solution is flung to by conventional method, and powder is made in drying, which, can by the way that nano particle is made in lutein ester
So that it is dissolved in cold water or normal-temperature water, and increases the stability of lutein ester, to increase its application range, and convenient for fortune
Defeated and storage.But it is relatively low using more emulsifier, nutrition and safeties such as tweens in preparation process.Currently, molten with corn alcohol
Albumen is as food function Ingredients Carrier it has been reported that still dissolving release property, body absorption utilization and nutrition in active ingredient
There is also deficiencies, such as 103431156 A and CN 106420666A of Chinese patent CN to disclose a kind of corn alcohol for safety etc.
Molten protein nano particle and preparation method thereof and the preparation method for glycosylating zeins nano-carrier, are by corn respectively
Nano-carrier particle is made by squeezing spraying equipment in alcohol soluble protein and glycosylated alcohol soluble protein, but prepared particle is larger,
Do not have good water-soluble, release property and absorbability as lutein nanometer carrier, it is difficult to play the life of lutein in vivo
Manage function.Therefore, being had concurrently there is presently no one kind keeps lutein embedding rate height, dissolubility good and body absorption release utilization rate
The preparation method of high corn protein peptide load lutein nanometer grain.
Invention content
The object of the present invention is to provide one kind with embedding rate height, dissolubility is good, the yellow activity of leaf is high and body absorption utilizes
Rate is good using corn peptide as the preparation method of the lutein nanometer grain of carrier.
The preparation process of the present invention includes the following steps：
1, a kind of corn peptide is the preparation method of the lutein nanometer grain of carrier, it is characterized in that：This method includes the following steps：
（1）Raw material screening and pretreatment
A certain amount of corn protein powder is taken, is crushed to 60 ~ 80 mesh, spray pattern is uniformly added into 10 ~ 15% moisture, swollen 10 ~
12h；Corn protein powder after swollen is put into high-pressure sterilizing pot, in 105 ~ 125 DEG C of temperature, pressure 0.1 ~ 0.15MPa conditions
15 ~ 30min of lower pretreatment.
（2）The preparation of corn peptide
In the buffer solution for weighing the pH8.0 that above-mentioned pretreated corn protein powder is added to 10 ~ 20 times of volumes, egg is then added
The alkali protease of white powder quality 0.1% ~ 1.0% digests 2 ~ 4h under the conditions of 60 DEG C, boiling 10min enzyme deactivations under the conditions of 100 DEG C；
Enzymolysis liquid centrifuges 20min at 4000 r/min, then use molecular cut off be 8000 ~ 12000 dalton ultrafiltration membrane into
Row separation, separated solution are 120 ~ 160 DEG C in temperature, and charging rate is spray-dried under the conditions of being 10 ~ 20mL/min, obtains jade
Rice Gly-His-Lys are spare.
（3）Corn peptide loads the preparation of lutein nanometer grain
Lutein by purity more than or equal to 80% is added in the ethanol solution that volume fraction is 85%, adjusts lutein concentration
0.4 ~ 0.8mg/mL, ultrasonic dissolution 1min prepare lutein lysate；The another corn peptide for taking above-mentioned steps to prepare is dissolved into 85%
In ethanol solution, adjusts 10 ~ 20mg/mL of corn peptide concentration and stirring prepares corn peptide solution in 30 minutes；
Above-mentioned lutein lysate is mixed with isometric corn peptide solution, 5 ~ 10min of homogeneous at 20 ~ 40 MPa of pressure,
Cycle twice, takes supernatant that the PBS buffer solution of isometric pH4.0 ~ 6.0 is added, and rotary evaporation removes ethyl alcohol to get corn peptide
Lutein nanometer grain lotion is loaded, the grain size that corn peptide load lutein nanometer grain is measured through Particle Size Analyzer is less than 100nm, sweeps
It retouches Electronic Speculum SEM and is shown as dispersion ball-type nanoparticle structure.
Description of the drawings：
Fig. 1 corn peptides load lutein nanometer grain granularmetric analysis figure
Fig. 2 corn peptides load lutein nanometer grain microstructure scanning electron microscope (SEM) photograph
The present invention is compared with existing technology and is had the following advantages：
One, the nutritive value and body absorption utilization rate of lutein are significantly improved.
Using the zein small peptide with the functions such as anti-oxidant, blood pressure lowering and anticancer as the carrier of lutein, corn peptide and
Both lutein nutritionally has synergistic action effect, nutritive value higher.Corn peptide loads lutein nanometer grain granularity
It is smaller to be easier to absorb, and since the hydrophilic interaction of corn peptide significantly improves the dissolubility of lutein, under the conditions of gastro-intestinal Fluid
Lutein release rate is up to 80% or more in 6h.And then improve the body absorption and utilizing status of lutein.
Two, granularity is small, dissolubility is high, and stability is good.Corn peptide loads lutein nanometer grain grain size and is less than 100nm, grain size
Distribution is relatively narrow, distributed areas are relatively uniform, and form is consistent；Solubility is high in water, and it is molten that lutein can be improved in good dispersion
10 times of solution degree or more, water solubility significantly improves；Lutein is rolled into core-shell structure by corn peptide, improves the inside and outside of lutein
Stability.
Three, non-edible materials are not used, it is safe, food and health products can be act as and directly eaten, tradition is avoided
The residual of hazardous compound in nano carrier material.
Four, technique is advanced, simple, has a extensive future.Corn peptide is obtained using biological enzymolysis and membrane separation technique, is used
Advanced to be easy to industrial high-pressure homogeneous method preparation corn peptide load lutein nanometer grain, embedding rate is high and distributed
It is good.
Corn peptide and the added value of lutein, wide market can fully be improved.It is that a kind of nutritive value is high, stability
With the high corn peptide of the good and internal utilization rate of dissolubility-lutein composite Nano granule product.
Specific implementation mode
Specific implementation mode one：It is real that a kind of corn peptide is that the preparation method of the lutein nanometer grain of carrier passes through following steps
It is existing：
1, a kind of corn peptide is the preparation method of the lutein nanometer grain of carrier, it is characterized in that：This method includes the following steps：
（1）Raw material screening and pretreatment
A certain amount of corn protein powder is taken, 60 ~ 80 mesh are crushed to, 10 ~ 15% moisture, 10 ~ 12h of swollen is added in spray pattern；It will
Corn protein powder after swollen is put into high-pressure sterilizing pot, is located in advance under the conditions of 105 ~ 125 DEG C of temperature, 0.1 ~ 0.15MPa of pressure
Manage 15 ~ 30min.
（2）The preparation of corn peptide
It weighs above-mentioned pretreated corn protein powder to be added in the buffer solution of pH 8.0 of 10 ~ 20 times of volumes, then be added
The alkali protease of albumen powder quality 0.1% ~ 1.0% digests 2 ~ 4h under the conditions of 60 DEG C, and boiling 10min goes out under the conditions of 100 DEG C
Enzyme；Enzymolysis liquid centrifuges 20min at 4000 r/min, and it is the ultrafiltration of 8000 ~ 12000 dalton then to use molecular cut off
Film is filtered, and separating liquid is 120 ~ 160 DEG C in temperature, and charging rate is spray-dried under the conditions of being 10 ~ 20mL/min, obtains jade
Rice Gly-His-Lys are spare.
（3）Corn peptide loads the preparation of lutein nanometer grain
Lutein by purity more than or equal to 80% is added in the ethanol solution that volume fraction is 85%, adjusts lutein concentration
0.4 ~ 0.8mg/mL, ultrasonic dissolution 1min prepare lutein lysate；The another corn peptide for taking above-mentioned steps to prepare is dissolved into 85%
In ethanol solution, adjusts 10 ~ 20mg/mL of corn peptide concentration and stirring prepares corn peptide solution in 30 minutes.
Above-mentioned lutein lysate is mixed with isometric corn peptide solution, at 20 ~ 40 Mpa of pressure position homogeneous 5 ~
10min, cycle is twice.Take supernatant to be added the PBS buffer solution of isometric pH4.0 ~ 6.0, rotary evaporation remove ethyl alcohol to get
Corn peptide loads lutein nanometer grain, and the grain size that corn peptide load lutein nanometer grain is measured through Particle Size Analyzer is less than 100nm,
Scanning electron microscope sem is shown as dispersion ball-type nanoparticle structure.
Specific implementation mode two：The difference of present embodiment and specific implementation mode one is step（1）In be crushed to
80 mesh, spray pattern are uniformly added into 15% moisture, swollen 12h.Other steps are same as the specific embodiment one.
Specific implementation mode three：The difference of present embodiment and specific implementation mode one is step（1）In in temperature
115 ~ 125 DEG C, 20 ~ 30min is pre-processed under the conditions of 0.1 ~ 0.15MPa of pressure.Other steps are same as the specific embodiment one.
Specific implementation mode four：The difference of present embodiment and specific implementation mode one is step（2）Middle corn egg
White powder is added in the buffer solution of pH 8.0 of 10 times of volumes, the alkali protease of albumen powder quality 0.5% is then added, 60
3h is digested under the conditions of DEG C.Other steps are same as the specific embodiment one.
Specific implementation mode five：The difference of present embodiment and specific implementation mode one is step（2）Use retention
Molecular weight be 8000 ~ 10000 dalton ultrafiltration membrane detached, solution temperature be 120 ~ 150 DEG C, charging rate be 15 ~
It is spray-dried under the conditions of 20mL/min.Other steps are same as the specific embodiment one.
Specific implementation mode six：The difference of present embodiment and specific implementation mode one is step（3）Middle adjustment leaf
Flavine 0.4 ~ 0.6mg/mL of concentration, ultrasonic dissolution 1min prepare lutein lysate；The another corn peptide for taking above-mentioned steps to prepare is molten
In solution to 85% ethanol solution, adjusts 15 ~ 20mg/mL of corn peptide concentration and stirring prepares corn peptide solution in 30 minutes.Other steps
It is rapid same as the specific embodiment one.
Specific implementation mode seven：The difference of present embodiment and specific implementation mode one is step（4）In in pressure
5 ~ 10min of homogeneous under 20 ~ 40 MPa, cycle is twice.Other steps are same as the specific embodiment one.
Claims (4)
1. a kind of corn peptide is the preparation method of the lutein nanometer grain of carrier, it is characterized in that：This method includes the following steps：
（1）Raw material screening and pretreatment
A certain amount of corn protein powder is taken, 60 ~ 80 mesh are crushed to, 10 ~ 15% moisture, 10 ~ 12h of swollen, after swollen is added
Corn protein powder is put into high-pressure sterilizing pot, under the conditions of 105 ~ 125 DEG C of temperature, 0.1 ~ 0.15MP of pressure pre-process 15 ~
30min；
（2）The preparation of corn peptide
It weighs above-mentioned corn protein powder to be added in the buffer solution of pH 8.0 of 10 ~ 20 times of volumes, protease is then added, 60
2 ~ 4h is digested under the conditions of DEG C, boiling 10min enzyme deactivations under the conditions of 100 DEG C, enzymolysis liquid centrifuges 20min at 4000 r/min, so
It is detached afterwards using ultrafiltration membrane, solution is 120 ~ 160 DEG C in temperature, and charging rate is sprayed dry under the conditions of being 10 ~ 20mL/min
It is dry, it is spare to obtain corn Gly-His-Lys；
（3）Corn peptide loads the preparation of lutein nanometer grain
Lutein by purity more than or equal to 80% is added in the ethanol solution that volume fraction is 85%, adjusts lutein concentration
0.4 ~ 0.8mg/mL, ultrasonic dissolution 1min prepare lutein lysate, and corn peptide prepared by above-mentioned steps is separately taken to be dissolved into 85%
In ethanol solution, adjusts 10 ~ 20mg/mL of corn peptide concentration and stirring prepares corn peptide solution in 30 minutes, above-mentioned lutein is molten
Solution liquid is mixed with isometric corn peptide solution, and 5 ~ 10min of homogeneous at 20 ~ 40 Mpa of pressure, cycle twice, takes supernatant to add
Enter the PBS buffer solution of isometric pH4.0 ~ 6.0, rotary evaporation removes ethyl alcohol and loads lutein nanometer grain, warp to get corn peptide
The grain size that Particle Size Analyzer measures corn peptide load lutein nanometer grain is less than 100nm, and scanning electron microscope sem is shown as dispersion ball-type
Nanoparticle structure.
2. a kind of corn peptide according to claim 1 is the preparation method of the lutein nanometer grain of carrier, it is characterized in that：
In the raw material screening and pre-treatment step, 10 ~ 15% moisture is uniformly added into spray pattern.
3. a kind of corn peptide according to claim 1 is the preparation method of the lutein nanometer grain of carrier, it is characterized in that：
In the preparation process of the corn peptide, the alkali protease of albumen powder quality 0.1% ~ 1.0%, ultrafiltration retaining molecular weight is added
For 8000 ~ 12000 dalton.
4. a kind of corn peptide according to claim 1 is the preparation method of the lutein nanometer grain of carrier, it is characterized in that：
In the preparation process of the corn peptide load lutein nanometer grain, the particle size range that corn peptide loads lutein nanometer grain is 50
~100nm。
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| CN112042937B (en) * | 2020-09-17 | 2023-11-21 | 青岛农业大学 | Water-soluble lutein emulsion gel and preparation method thereof |
| CN115590194A (en) * | 2022-10-27 | 2023-01-13 | 江南大学（Cn） | Preparation method of lutein-loaded soybean peptide with high loading capacity and high stability |
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