WO2016088275A1 - 高効率エタノール発酵菌 - Google Patents
高効率エタノール発酵菌 Download PDFInfo
- Publication number
- WO2016088275A1 WO2016088275A1 PCT/JP2014/082332 JP2014082332W WO2016088275A1 WO 2016088275 A1 WO2016088275 A1 WO 2016088275A1 JP 2014082332 W JP2014082332 W JP 2014082332W WO 2016088275 A1 WO2016088275 A1 WO 2016088275A1
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- ethanol
- gene
- strain
- xylose
- highly efficient
- Prior art date
Links
Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
- C12N1/145—Fungal isolates
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
- C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
- C12P7/06—Ethanol, i.e. non-beverage
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
- C12N1/16—Yeasts; Culture media therefor
- C12N1/165—Yeast isolates
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/01—Preparation of mutants without inserting foreign genetic material therein; Screening processes therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/02—Preparation of hybrid cells by fusion of two or more cells, e.g. protoplast fusion
- C12N15/04—Fungi
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
- C12N15/90—Stable introduction of foreign DNA into chromosome
- C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
- C12N15/905—Stable introduction of foreign DNA into chromosome using homologous recombination in yeast
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
- C12N1/16—Yeasts; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/645—Fungi ; Processes using fungi
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/645—Fungi ; Processes using fungi
- C12R2001/84—Pichia
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
- Y02E50/00—Technologies for the production of fuel of non-fossil origin
- Y02E50/10—Biofuels, e.g. bio-diesel
Definitions
- ethanol is efficiently produced from pentose sugar (hereinafter sometimes referred to as C5 sugar) and hexose sugar (hereinafter sometimes referred to as C6 sugar). Relates to microorganisms that can.
- C5 sugar pentose sugar
- C6 sugar hexose sugar
- Bioethanol is expected as a non-depleting renewable resource produced from biomass.
- carbon dioxide generated by burning bioethanol is carbon neutral, it is considered that the increase in carbon dioxide, which is a major cause of global warming, is suppressed by the use of bioethanol.
- xylose is a sugar that is contained in the biomass next to glucose, and the efficient use of pentose sugar is a major issue in bioethanol production.
- Patent Document 1 discloses an invention in which xylose (C5 sugar) is converted to xylulose by introducing a gene having xylose transporter activity into a host cell, incorporated into a glycolytic pentose phosphate pathway, and used for fermentation. Has been.
- Non-Patent Document 1 discloses that xylose utilization ability is imparted by incorporating a xylose utilization gene derived from Escherichia coli into Zymomonas.
- Non-Patent Document 2 describes that Pichia yeasts produce ethanol using xylose.
- Patent Document 1 introduces a protein having a xylose transporter activity derived from Candida guilliermondii into Saccharomyces cerevisiae as a host. That is, a foreign gene is introduced.
- Patent Document 2 is also an invention in which different types of genes are introduced into the host, although the transporter genes are different.
- Non-Patent Document 1 introduces a xylose-utilizing gene. Although the technical idea is different from that of Patent Documents 1 and 2, it is not different from introducing a foreign gene.
- Patent Documents 1 and 2 and Non-Patent Document 1 are both implemented in Japan to implement the “Cartagena Protocol on Biosafety of the Convention on Biological Diversity” adopted by the United Nations. It is necessary to take containment measures in accordance with the so-called Cartagena Act. Therefore, since a facility for assuring biosafety is required, it is disadvantageous in terms of cost to produce ethanol using the cells.
- An object of the present invention is to obtain a high-efficiency ethanol-fermenting bacterium having high ethanol production efficiency without introducing a foreign gene.
- the high-efficiency ethanol-fermenting bacterium of the present invention is a fermenting bacterium that efficiently produces ethanol from pentose and hexose, and has a deposit number of NITE BP-01963 at the Patent Microorganism Depository Center. It is characterized by being deposited as.
- transaldolase gene The transaldolase gene, alcohol dehydrogenase gene, and pyruvate decarboxylase gene are all Meyerozyma guilliermondi genes.
- the highly efficient ethanol-fermenting bacterium of the present invention can obtain higher ethanol production efficiency than the parent strain without introducing a foreign gene.
- the wild strain of Meyerozyma guilliermondi an ascomycete yeast
- ammonia-treated rice straw-derived enzyme saccharified solution for example, one obtained as follows can be used. First, rice straw produced in Kumagaya City, Saitama Prefecture is immersed in an equal amount of 25% by mass ammonia water at a temperature of 80 ° C. for 3 hours, and then pretreated by releasing ammonia. Next, after adjusting the pH of the pretreated rice straw, saccharification enzyme (Meiji Seika Pharma Co., Ltd., trade name: Acremonium Cellulase) is added and maintained at a temperature of 50 ° C. for 72 hours for enzymatic saccharification. And a slurry containing the enzyme saccharified solution is obtained.
- saccharification enzyme Meiji Seika Pharma Co., Ltd., trade name: Acremonium Cellulase
- the ammonia-treated rice straw-derived enzyme saccharified solution contains, for example, 3 to 15% by mass of glucose and 1 to 10% by mass of xylose.
- mutagen examples include ethylating agents such as N-ethyl-N-nitrosourea (ENU) and ethyl methanesulfonate (EMS), and base analogs such as 5-bromo-2'-deoxyuridine (BrdU). Nitroso compounds such as nitroamine and nitrosoguanidine can be used.
- ethylating agents such as N-ethyl-N-nitrosourea (ENU) and ethyl methanesulfonate (EMS)
- base analogs such as 5-bromo-2'-deoxyuridine (BrdU).
- Nitroso compounds such as nitroamine and nitrosoguanidine can be used.
- the BP-01962 strain is a mutant strain obtained by acclimating and culturing the parent strain in a medium obtained by adding a mutagen to the ammonia-treated rice straw-derived enzyme saccharified solution, and repeatedly selecting bacteria that grow on the medium. . Therefore, the BP-01962 strain has improved xylose utilization and ethanol fermentation performance without introducing foreign genes compared to the wild strain or N strain of Meyerozyma guilliermondi.
- the high-efficiency ethanol-fermenting bacteria of the present embodiment in which the self-cloned TAL gene, ADH gene, and PDC gene are further introduced into the BP-01962 strain, have been obtained by the Applicant from the National Institute of Technology and Evaluation of the National Institute of Technology and Evaluation, This is deposited in Japan at room 2-5-8, Kazusa Kamashichi, Kisarazu City, Chiba Prefecture, 292-0818, Japan.
- the acceptance date is November 19, 2014, and the acceptance number is NITE BP-01963.
- the BP-01963 strain has further improved xylose utilization and ethanol fermentation performance without introducing a foreign gene as compared to the BP-01962 strain or the N strain.
- gene + terminator part PCR amplification of the gene to be introduced and its terminator part (hereinafter referred to as gene + terminator part). Amplify the promoter part to be used for introduction. All of these are PCR-amplified from the chromosome of Meyerozyma guilliermondi, the strain used in the present invention.
- transaldolase + terminator may be used as the DNA fragment for homologous recombination. This is because it is considered that transaldolase acts efficiently by using a promoter of xylose reductase that functions during xylose utilization.
- the promoter of xylose reductase was amplified using the primers of SEQ ID NO: 1 and SEQ ID NO: 2, and the transaldolase gene and terminator part were amplified using the primers of SEQ ID NO: 3 and 4 below.
- Sequence number 1 AAGGCTTGGGAACTTTCTTT Sequence number 2: AGCAATTGATGATTAATTTT Sequence number 3: ATGACCAATTCTCTTGAACA Sequence number 4: AAATTGTGCCGTGTCAAACT
- a GAPDH promoter alcohol dehydrogenase + terminator may be used. Since GAPDH is a strong promoter present in the glycolytic system, it is considered that GAPDH acts efficiently by using it as a promoter for alcohol dehydrogenase, which is a glycolytic enzyme.
- Alcohol dehydrogenase has an action of converting acetaldehyde into ethanol and produces NAD + when it is NADH-dependent, and therefore has an action of enhancing the action of NAD + -dependent xylitol dehydrogenase.
- the GAPDH promoter was amplified using the primers of SEQ ID NO: 5 and SEQ ID NO: 6 below, and the alcohol dehydrogenase gene and terminator part were amplified using the primers of SEQ ID NOs: 7 and 8 below.
- Sequence number 5 GTTGTAGCGGAGGCTCAATT Sequence number 6: TGTATAATTTAAATGTGGGT Sequence number 7: ATGTCAATTCCAGAATCCAT Sequence number 8: CACCTTGGCTGGAAGTGCTG
- SEQ ID NO: 9 a DNA fragment obtained by introducing a GAPDH promoter amplified with the primers of SEQ ID NO: 5 and SEQ ID NO: 6 is used for homologous recombination. went.
- the sequence of SEQ ID NO: 9 represents the end of the promoter of the PDC gene
- the sequence of SEQ ID NO: 10 represents the start of the PDC gene.
- the dilute sulfuric acid-treated corn stover-derived enzyme saccharified solution used was obtained as follows. First, a corn stover produced in Iowa, USA is immersed in a double amount of 3.7% by mass sulfuric acid at a temperature of 170 ° C. for 10 minutes, and then pretreated by returning to normal temperature. Next, an aqueous NaOH solution is added to the pretreated corn stover to adjust the pH to 4, and then a saccharification enzyme (manufactured by Meiji Seika Pharma Co., Ltd., trade name: Acremonium Cellulase) is added and maintained at a temperature of 50 ° C. for 72 hours. Then, enzymatic saccharification is performed to obtain a slurry containing the enzymatic saccharified solution.
- a saccharification enzyme manufactured by Meiji Seika Pharma Co., Ltd., trade name: Acremonium Cellulase
- the slurry was subjected to solid-liquid separation by centrifugation, and the collected liquid was adjusted to pH 6 with an aqueous NaOH solution to obtain the diluted saccharified corn stover-derived enzyme saccharified solution.
- the dilute sulfuric acid-treated corn stover-derived enzyme saccharified solution contains, for example, 3 to 15% by mass of glucose and 1 to 10% by mass of xylose.
- the dilute sulfuric acid-treated corn stover-derived enzyme saccharified solution contained glucose 45 g / L and xylose 38 g / L, and had a pH of 6. Then, after the culture, the medium was collected, the ethanol concentration was measured by GC-FID (manufactured by GL Sciences, trade name: GC390B), and the fermentation yield was calculated by the following formula (1). The results are shown in FIG.
- an enzyme saccharified solution derived from 26% by mass of dilute sulfuric acid-treated corn stover is used as a medium, and a culture solution of Meyerozyma guilliermondi N strain is added to the medium so that the OD 600 of the medium is 0.5, and the temperature is 30 ° C. Culture was performed for 100 hours.
- the diluted sulfuric acid-treated corn stover-derived enzyme saccharified solution had a pH of 6 including glucose 64 g / L and xylose 48 g / L.
- the culture medium was collected after the culture, and the ethanol concentration was measured by GC-FID (manufactured by GL Science Co., Ltd., trade name: GC390B), and the fermentation yield was calculated by formula (1).
- the results are shown in FIG.
- FIG. 2 shows that the total amount of glucose and xylose is digested 120 hours after the start of the culture, and the ethanol concentration increases as the culture time increases.
- the glucose concentration was almost zero 48 hours after the start of the culture, but since then the xylose concentration decreased and the ethanol concentration continued to increase, the BP-01963 strain digested all the glucose. After that, it is clear that ethanol fermentation is performed using xylose as a substrate.
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Biotechnology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- Mycology (AREA)
- Molecular Biology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Plant Pathology (AREA)
- Botany (AREA)
- Medicinal Chemistry (AREA)
- Tropical Medicine & Parasitology (AREA)
- Virology (AREA)
- Cell Biology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
Description
配列番号2:AGCAATTGATGATTAATTTT
配列番号3:ATGACCAATTCTCTTGAACA
配列番号4:AAATTGTGCCGTGTCAAACT
また、GAPDHのプロモーター、アルコールデヒドロゲナーゼ+ターミネーターを用いると良い。GAPDHは解糖系に存在する強力なプロモータであることから、解糖系の酵素であるアルコールデヒドロゲナーゼのプロモーターとして使用することにより、効率よく作用するものと考えられる。アルコールデヒドロゲナーゼはアセトアルデヒドをエタノールに変換する作用を持つとともに、NADH依存の場合にはNAD+を生産するため、NAD+依存のキシリトールデヒドロゲナーゼの作用を強化する作用がある。
配列番号6:TGTATAATTTAAATGTGGGT
配列番号7:ATGTCAATTCCAGAATCCAT
配列番号8:CACCTTGGCTGGAAGTGCTG
PDC遺伝子は、PDC遺伝子のプロモーターをGAPDHのプロモーターに置換することにより強化した。配列番号9および配列番号10で表される配列の間に、配列番号5および配列番号6のプライマーで増幅されるGAPDHのプロモーターを導入することで得られるDNA断片を相同組換することで置換を行った。配列番号9の配列がPDC遺伝子のプロモーターの終端、配列番号10の配列がPDC遺伝子の始端を表す。
配列番号10:ATGACAGAAATTACTTTGGG
また、この方法により得られた菌株は、遺伝子を導入しているが、セルフクローニングであるため、カルタヘナ法上、非組換菌扱いになる範疇に属するものとなっている。
・・・(1)
(グルコース濃度及びキシロース濃度は培養開始前の初期濃度である)
次に、20質量%の希硫酸処理コーンストーバ由来酵素糖化液を培地とし、該培地にBP-01962株の培養液を培地のOD600が0.5となるように添加し、30℃の温度で100時間培養を行った。前記希硫酸処理コーンストーバ由来酵素糖化液は、グルコース64g/L、キシロース48g/Lを含みpH6であった。そして、前記培養後に前記培地を採取してエタノールの濃度をGC-FID(ジーエルサイエンス株式会社製、商品名:GC390B)により測定し、式(1)により発酵収率を算出した。結果を図1に示す。
Claims (2)
- 五炭糖及び六炭糖から効率的にエタノールを産生する発酵菌であって、
特許微生物寄託センターに受託番号NITE BP-01963として寄託されていることを特徴とする高効率エタノール発酵菌。 - 請求項1に記載の高効率エタノール発酵菌において、特許微生物寄託センターに受託番号NITE BP-01962として寄託されている高効率エタノール発酵菌に、セルフクローニングしたトランスアルドラーゼ遺伝子、アルコールデヒドロゲナーゼ遺伝子及び、ピルビン酸デカルボキシラーゼ遺伝子を導入したことを特徴とする高効率エタノール発酵菌。
Priority Applications (6)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US15/532,814 US10059965B2 (en) | 2014-12-05 | 2014-12-05 | Highly efficient ethanol-fermentative yeast |
BR112017010660A BR112017010660A2 (pt) | 2014-12-05 | 2014-12-05 | levedura fermentativa de etanol altamente eficiente e uso da mesma |
EP14907566.5A EP3228698B1 (en) | 2014-12-05 | 2014-12-05 | Highly efficient ethanol-fermentative bacteria |
PCT/JP2014/082332 WO2016088275A1 (ja) | 2014-12-05 | 2014-12-05 | 高効率エタノール発酵菌 |
JP2016562191A JP6145583B2 (ja) | 2014-12-05 | 2014-12-05 | 高効率エタノール発酵菌 |
CN201480083840.7A CN107429219B (zh) | 2014-12-05 | 2014-12-05 | 高效乙醇发酵菌 |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
PCT/JP2014/082332 WO2016088275A1 (ja) | 2014-12-05 | 2014-12-05 | 高効率エタノール発酵菌 |
Publications (1)
Publication Number | Publication Date |
---|---|
WO2016088275A1 true WO2016088275A1 (ja) | 2016-06-09 |
Family
ID=56091246
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/JP2014/082332 WO2016088275A1 (ja) | 2014-12-05 | 2014-12-05 | 高効率エタノール発酵菌 |
Country Status (6)
Country | Link |
---|---|
US (1) | US10059965B2 (ja) |
EP (1) | EP3228698B1 (ja) |
JP (1) | JP6145583B2 (ja) |
CN (1) | CN107429219B (ja) |
BR (1) | BR112017010660A2 (ja) |
WO (1) | WO2016088275A1 (ja) |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP2020115827A (ja) * | 2019-01-28 | 2020-08-06 | Jxtgエネルギー株式会社 | キシリトールの蓄積を抑制した酵母 |
Families Citing this family (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US10119146B2 (en) * | 2014-12-05 | 2018-11-06 | Honda Motor Co., Ltd. | Highly efficient ethanol-fermentative yeast |
Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP2011024500A (ja) * | 2009-07-27 | 2011-02-10 | Toyota Central R&D Labs Inc | 発酵能力が向上された酵母及びその利用 |
WO2011065539A1 (ja) * | 2009-11-30 | 2011-06-03 | 国立大学法人神戸大学 | バイオマスからのエタノールの生産方法 |
JP2011193788A (ja) * | 2010-03-19 | 2011-10-06 | Toyota Central R&D Labs Inc | 発酵能力が向上された酵母及びその利用 |
Family Cites Families (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN100359017C (zh) * | 1991-03-18 | 2008-01-02 | 佛罗里达大学研究基金会 | 通过重组宿主生产乙醇 |
US7846712B2 (en) | 2006-06-01 | 2010-12-07 | Alliance For Sustainable Energy, Llc | L-arabinose fermenting yeast |
JP2012170422A (ja) | 2011-02-23 | 2012-09-10 | Kyoto Institute Of Technology | キシローストランスポーター活性を有する新規タンパク質および当該タンパク質をコードするポリヌクレオチド、並びにそれらの利用 |
WO2013061941A1 (ja) * | 2011-10-24 | 2013-05-02 | トヨタ自動車株式会社 | 組換え酵母を用いたエタノールの製造方法 |
-
2014
- 2014-12-05 BR BR112017010660A patent/BR112017010660A2/pt not_active Application Discontinuation
- 2014-12-05 WO PCT/JP2014/082332 patent/WO2016088275A1/ja active Application Filing
- 2014-12-05 JP JP2016562191A patent/JP6145583B2/ja active Active
- 2014-12-05 CN CN201480083840.7A patent/CN107429219B/zh not_active Expired - Fee Related
- 2014-12-05 US US15/532,814 patent/US10059965B2/en not_active Expired - Fee Related
- 2014-12-05 EP EP14907566.5A patent/EP3228698B1/en not_active Not-in-force
Patent Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP2011024500A (ja) * | 2009-07-27 | 2011-02-10 | Toyota Central R&D Labs Inc | 発酵能力が向上された酵母及びその利用 |
WO2011065539A1 (ja) * | 2009-11-30 | 2011-06-03 | 国立大学法人神戸大学 | バイオマスからのエタノールの生産方法 |
JP2011193788A (ja) * | 2010-03-19 | 2011-10-06 | Toyota Central R&D Labs Inc | 発酵能力が向上された酵母及びその利用 |
Non-Patent Citations (3)
Title |
---|
CSEAR FONSECA ET AL.: "L-Arabinose metabolism in Candida arabinofermentans PYCC 5603T and Pichia guilliermondii PYCC 3012: influence of sugar and oxygen on product formation", APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, vol. 75, no. 2, 2007, pages 303 - 310, XP019513655, DOI: doi:10.1007/s00253-006-0830-7 * |
HAN LI-LI ET AL.: "Breeding of Higher Ethanol Fermentation of Xylose Strain with Protoplast Fusion and Mutagenisis", LIQUOR MAKING, vol. 35, no. 2, 2008, pages 38 - 41, XP008185458 * |
T.GRANSTROM ET AL.: "Chemostat study ot xylitol production by Candida guilliermondii", APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, vol. 55, 2001, pages 36 - 42, XP055373730 * |
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP2020115827A (ja) * | 2019-01-28 | 2020-08-06 | Jxtgエネルギー株式会社 | キシリトールの蓄積を抑制した酵母 |
JP7365770B2 (ja) | 2019-01-28 | 2023-10-20 | Eneos株式会社 | キシリトールの蓄積を抑制した酵母 |
Also Published As
Publication number | Publication date |
---|---|
BR112017010660A2 (pt) | 2017-12-26 |
EP3228698B1 (en) | 2019-08-14 |
US10059965B2 (en) | 2018-08-28 |
JP6145583B2 (ja) | 2017-06-14 |
US20170369906A1 (en) | 2017-12-28 |
JPWO2016088275A1 (ja) | 2017-05-25 |
EP3228698A4 (en) | 2018-05-23 |
CN107429219A (zh) | 2017-12-01 |
EP3228698A1 (en) | 2017-10-11 |
CN107429219B (zh) | 2020-08-25 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
CN105121637B (zh) | 酿酒酵母中替代甘油形成的消耗电子的乙醇生产途径 | |
JP6087854B2 (ja) | 組換え酵母を用いたエタノールの製造方法 | |
JP2010239925A (ja) | キシロースを利用して有用物質を生産する方法 | |
CN103459588B (zh) | 克鲁维酵母属的突变体酵母和使用了该突变体酵母的乙醇的制造方法 | |
JP6228323B2 (ja) | 高効率エタノール発酵菌 | |
CN111712576A (zh) | 微生物菌株及其用途 | |
JP6145583B2 (ja) | 高効率エタノール発酵菌 | |
JP6240344B2 (ja) | 高効率エタノール発酵菌 | |
JP6180660B2 (ja) | 高効率エタノール発酵菌 | |
JP5845210B2 (ja) | 組換え酵母、及びそれを用いたエタノールの製造方法 | |
JP6145582B2 (ja) | 高効率エタノール発酵菌 | |
JP6249391B2 (ja) | キシロースを高温で発酵する方法 |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
121 | Ep: the epo has been informed by wipo that ep was designated in this application |
Ref document number: 14907566 Country of ref document: EP Kind code of ref document: A1 |
|
DPE1 | Request for preliminary examination filed after expiration of 19th month from priority date (pct application filed from 20040101) | ||
ENP | Entry into the national phase |
Ref document number: 2016562191 Country of ref document: JP Kind code of ref document: A |
|
REEP | Request for entry into the european phase |
Ref document number: 2014907566 Country of ref document: EP |
|
REG | Reference to national code |
Ref country code: BR Ref legal event code: B01A Ref document number: 112017010660 Country of ref document: BR |
|
WWE | Wipo information: entry into national phase |
Ref document number: 15532814 Country of ref document: US |
|
NENP | Non-entry into the national phase |
Ref country code: DE |
|
ENP | Entry into the national phase |
Ref document number: 112017010660 Country of ref document: BR Kind code of ref document: A2 Effective date: 20170522 |