WO2015199480A1

WO2015199480A1 - Feed additive composition for enhancing growth and development of shrimp, comprising, as active ingredient, mixture of turmeric and stevia

Info

Publication number: WO2015199480A1
Application number: PCT/KR2015/006532
Authority: WO
Inventors: 이우송; 류영배; 정형재; 노문철; 박수진; 김영민; 박지영; 권형준
Original assignee: 한국생명공학연구원
Priority date: 2014-06-26
Filing date: 2015-06-26
Publication date: 2015-12-30
Also published as: KR20160007735A; KR101716306B1

Abstract

The present invention relates to: a feed additive composition for enhancing the growth and development of shrimp, the composition comprising, as an active ingredient, a mixture of turmeric and stevia or stevioside; and a feed comprising the composition. The mixture of the present invention is included in a feed additive and thus remarkably enhances the growth and development of shrimp. Simultaneously, due to having a function of purifying the quality of water for farming shrimp, the mixture can reduce the rate of mortality caused by water quality deterioration. Therefore, the mixture can be more usefully used in the shrimp farming industry.

Description

Shrimp growth promoting feed additive composition comprising a mixture of turmeric and stevia as an active ingredient

The present invention relates to a feed additive composition for improving shrimp growth comprising turmeric and a mixture of stevia or stevioside as an active ingredient and a feed comprising the same.

In recent years, the fish farming industry suffers from the deterioration of the farming environment and the increase of stress caused by the pollution of the water environment, which leads to a decrease in the activity of the fish, a decrease in the meat quality, and a misuse of antibiotics. In particular, the accumulation of antibiotics in the fish body, the proliferation of antibiotic resistant strains, and the contamination of water due to the misuse of antibiotics are a series of problems. Therefore, new additives for natural immunity enhancement and disease prevention using natural raw materials are necessary at this time. .

On the other hand, seafood, such as shrimp, a typical crustacean fish species, is increasing in quality and quantity on a daily basis due to an increase in national income and interest in health. In particular, shrimp farming is carried out at festival farms in coastal or estuary areas, and these are seriously affected by viral diseases caused by the inflow of seawater. Onshore shrimp farming facilities are increasing in Korea to prevent the influx of viruses, but in the currently operating farms, food wastes and excreta accumulate due to continuous feeding, resulting in odor due to deterioration of water quality. Although a large amount of mortality appears due to the increased concentrations of ammonia, nitrite and nitrate, which are the main causes, the development of shrimp-only aquaculture feed additives has not been made at all.

In view of this, there is a great need for the development of aquaculture feed for shrimp that can prevent disease while having better feed efficiency than high-performance feed additives or imported feed.

The present inventors have confirmed that by mixing turmeric and stevia or stevioside in a constant ratio and using it as a feed additive, the present invention can simultaneously show not only the growth promoting effect of shellfish, especially shrimp, but also the effect of purifying the water quality of cultured water. Was completed.

One object of the present invention is to provide a feed additive composition for shrimp growth, comprising turmeric and a mixture of stevia or stevioside as an active ingredient.

Another object of the present invention is to provide a feed comprising the feed additive composition.

Still another object of the present invention is to provide a feed additive composition for improving shrimp growth and purifying water quality of aquaculture comprising turmeric and a mixture of stevia or stevioside as an active ingredient.

It is yet another object of the present invention to provide a method for enhancing shrimp growth comprising feeding turmeric and a mixture of stevia or stevioside to shrimp.

It is yet another object of the present invention to provide a method of improving the water quality of cultured water comprising administering turmeric and a mixture of stevia or stevioside to shrimp farmed water.

The mixture of turmeric and stevia or stevioside of the present invention is included in the feed additive to significantly improve the growth of the shrimp and at the same time has the water purification function of the shrimp cultured water, which can reduce the mortality caused by water degradation. It can be used more usefully in industry.

1 is a photograph showing the appearance of shrimp and aquaculture farms fed feed containing turmeric and stevia or stevioside as an active ingredient.

As one aspect for solving the above problems, the present invention provides a feed additive composition for shrimp growth, comprising turmeric and a mixture of stevia or stevioside as an active ingredient.

In the present invention, "Turmeric" is a perennial herbaceous plant belonging to the genus Ginger and Cucumber, and can be used in various ways for medicinal, edible, dyed, or ornamental. Specific examples of turmeric include Curcuma wenyujin , YH Chen et C. Ling, Curcuma longa Linne, Curcuma longa Salisb, Curcuma zedoaria ( Curcuma kwansiensis , SG Lee et CF Liang), acu ( curcuma aeruginosa ), bonsai ( Curcuma phaeocaulis Val.) Curcuma domestica , but there are limitations no. In the present invention, turmeric may be purchased and used commercially, or may be one that is collected or cultivated in nature, and may mainly use a root portion, but is not limited thereto. In addition, the dried dried turmeric, or dried bark turmeric dried by removing the bark thereof and dried may be used in powder form.

In the present invention, "Stevia ( stevia rebaudiana Bertoni ), perennial perennial herb belonging to the Asteraceae can be used in herbal tea, beverages, herbal preparations, health supplements and the like. Stevia leaves also contain large amounts of sweet flavoring ingredients, such as stevioside and rebaudioside, which can be used as natural sweeteners. In the present invention, stevia may be purchased and used commercially available, or may be one that is harvested or grown in nature, it is preferable to use mainly the leaf portion, but is not limited thereto. In the present invention, stevia may be used as it is, or dried form as it is dried, or dried stevia dried by steaming it may be used in powder form.

In the present invention, "stevioside" is a natural polysaccharide material mainly contained in stevia leaves, specifically, steviosides A1, A2, steviol 1,2,3, dulcoside, rebaudioside a and various forms of sugars such as b, c, e, and f. The stevioside of the present invention may be purchased and used commercially, or may be used extracted and separated from stevia collected or grown in nature. At this time, a well-known method can be used for the extraction and separation method of stevioside.

The feed additive composition of the present invention may include turmeric and a mixture of stevia or stevioside as an active ingredient for promoting growth of shrimp, and the mixture may be present in powder form.

The feed additive composition of the present invention may be prepared by adding turmeric and a mixture of stevia or stevioside in an appropriate effective concentration range according to various preparation methods known in the art. Specifically, the feed additive composition of the present invention may include turmeric and a mixture of stevia or stevioside in a ratio of 0.01 to 100% by weight of the total feed additive composition, but is not limited thereto.

When the turmeric and stevia mixture is included as an active ingredient of the feed additive composition, the mixing ratio thereof may be arbitrarily selected, but preferably 1:10 to 10: 1, more preferably 1: 1 to 1: 3. Can be mixed in proportions.

In addition, when the turmeric and stevioside mixture is included as an active ingredient of the feed additive composition, the mixing ratio thereof may be arbitrarily selected, preferably 1: 100 to 100: 1, more preferably 1:10 to 10. Can be mixed in a ratio of 1: 1.

In an experimental example of the present invention, a feed additive composition in which turmeric and stevia powder were mixed at a weight ratio of 3: 7 was prepared and used, and a feed containing a feed additive containing a mixture of turmeric and stevia in the mixed ratio had acute toxicity. Although not shown (Experimental Example 1), by increasing the weight gain and survival rate significantly, the growth promoting effect of the shrimp fed the feed was confirmed (Experimental Example 2).

Feed additive composition of the present invention can improve the water quality of cultured water by including turmeric and a mixture of stevia or stevioside as an active ingredient.

In an experimental example of the present invention, the feed additive composition in which turmeric and stevia powder were mixed at a weight ratio of 3: 7 was prepared and used, and the feed was added to the feed additive containing the mixture of turmeric and stevia in the mixing ratio. In the culture tank, it was confirmed that suspended solids, chemical oxygen demand, total nitrogen content and nitrite nitrogen were derived at a significantly lower value than the feed water containing the mixture (Experimental Example 4).

Therefore, based on the excellent water quality improvement effect of the mixture of turmeric and stevia, the problems of the existing aquaculture feed developed without considering the water pollution and the dissolved oxygen in the water, which are the most important environmental factors in the farming environment, are simultaneously improved. can do.

The feed additive composition of the present invention further includes a substance added to the feed for various effects such as supplementation of nutrients and prevention of weight loss, enhancement of digestive availability of fiber in the feed, improvement of oil quality, prevention of reproduction disorder and improvement of conception, and prevention of high temperature stress in summer. It may include. The feed additive of the present invention corresponds to a feed supplement in the Feed Control Act, and includes mineral preparations such as sodium bicarbonate, bentonite, magnesium oxide, and composite minerals; Mineral preparations which are trace minerals such as zinc, copper, cobalt and selenium; Vitamin agents such as keratin, vitamins A, D, E, nicotinic acid, and vitamin B complexes; Protective amino acids such as methionine and lysine; Protective fatty acids such as fatty acid calcium salts; Probiotics (lactic acid bacteria), yeast culture, fungi such as fungi fermentation, yeast and the like may be further included. In addition, the feed additive composition of the present invention and the feed containing the same are plant protein feeds such as wheat, barley, corn, ground or crushed as auxiliary ingredients, animal protein feeds such as blood meal, meat meal, fish meal, animal fat and vegetable fat In addition to the active ingredient, it can be used with nutritional supplements, growth promoters, digestive absorption accelerators and disease prevention agents.

The feed additive composition of the present invention can be applied to a number of animal diets, ie feeds, including mammals, poultry, fish and shellfish.

Animal feed blending method comprising a feed additive composition according to the present invention can be mixed with animal feed in an amount of about 1g to 500g, preferably 10g to 100g per kg on a dry weight basis. In addition, the feed mixture may be thoroughly mixed and then fed into a mesh or subjected to a palletizing, expanding and extruding process through a further processing process.

In another aspect, the present invention provides a feed comprising the feed additive composition. The feed additive composition is included in the feed may not only improve the growth of the shrimp, but also improve the quality of the cultured water.

"Feed" in the present invention is any natural or artificial diet, meal or the like or any one of the above meals for the animal to eat, ingest, digest, or according to the present invention, and the mixture of turmeric and stevia or stevioside is effective. Feed containing feed additives as a component can be prepared in various forms of feed known in the art, and may preferably include rich feed, research feed and / or special feed.

The feed of the present invention may include the feed additive composition in a ratio of 0.01 to 10.0% by weight of the total feed, preferably may be included in a ratio of 0.5 to 5.0% by weight. In addition, turmeric and a mixture of stevia or stevioside may be included in the ratio of 0.001 to 5.0% by weight of the whole feed, preferably in the ratio of 0.5 to 3.5% by weight, more preferably 2.0% by weight. It may be included in the ratio of, but is not limited thereto.

In another aspect, the present invention provides a method for enhancing shrimp growth comprising feeding the feed additive composition, a feed or turmeric containing the same, and a mixture of stevia or stevioside to shrimp.

The present invention also provides a method of improving the water quality of the cultured water comprising the step of administering the feed additive composition, a feed or turmeric containing the same, and a mixture of stevia or stevioside to the shrimp farmed water.

The turmeric and stevia may be present in a mixing ratio of 1: 1 to 1: 3.

Hereinafter, the present invention will be described in more detail with reference to Examples. These examples are intended to illustrate the present invention more specifically, but the scope of the present invention is not limited by these examples.

제조예 1: 울금 및 스테비아의 혼합물(사료첨가제)의 제조Preparation Example 1 Preparation of Mixture of Turmeric and Stevia (Feed Additive)

Turmeric and stevia were washed and dried, and then ground to prepare a powder having a particle size of 50 to 100 mesh. Each prepared powder was mixed with a turmeric: stevia ratio of 3: 7 to prepare a mixture.

제조예 2: 울금 및 스테비아의 혼합물이 포함된 사료첨가제를 함유한 사료의 제조Preparation Example 2 Preparation of a Feed Containing a Feed Additive Containing a Mixture of Turmeric and Stevia

20 kg of the turmeric and stevia mixture prepared in Preparation Example 1 were mixed with a commercially available shrimp feed consisting of fish meal, starch, vitamins, minerals, and the like, and the content of the feed additive (functional additive) containing the mixture of turmeric and stevia To include 2% by weight, the shrimp farming feed of the present invention was prepared (Table 1).

Table 1

실험예 1: 울금 및 스테비아 혼합물이 포함된 사료첨가제를 함유한 사료 조성물의 급성 독성 실험Experimental Example 1: Acute Toxicity Test of Feed Composition Containing Feed Additives Containing Turmeric and Stevia Mixtures

In order to determine the acute toxicity of feed containing feed additives containing turmeric and stevia, 20,000 shrimp were dropped in aquaculture ponds (100 pyeong) and the toxicity of the preparation of Example 2 was administered for 10 days. It was.

Specifically, the shrimp (3.5-5.0 g / horse) was cultured for 10 days while administering the feed of Preparation Example 2 in a culture pond maintained at a water temperature of 27 ± 2 ℃. At this time, the farm was supplied with air using aberrations. Survival was calculated based on 100% of the control group fed the feed containing feed additives containing no turmeric and stevia mixture.

TABLE 2

As a result of the experiment, the feed of Preparation Example 2 containing feed additives containing turmeric and stevia mixture showed a survival rate of 93% in the experimental group administered for 10 days, confirming that there was no toxicity.

실험예 2: 울금 및 스테비아 혼합물이 포함된 사료첨가제를 함유한 사료의 새우 생육증진 효과Experimental Example 2: Shrimp growth effect of the feed containing the feed additive containing turmeric and stevia mixture

In order to examine the growth effect of the shrimp by the feed additive containing the turmeric and stevia mixture, the feed of the preparation example 2 containing the feed additive containing the turmeric and stevia mixture of 100 Weight gain and survival were measured.

Specifically, the aquarium for shrimp farming was carried out by publishing 100,000 fish fry in a 17m × 17m × 1.2m sized fish tank in a pond with automatic temperature control (27 ± 2.0 ℃), oxygen supply and aberration. The salinity of water was maintained at 5 ‰, and the blended feed was used in the feed prepared in Preparation Example 2. The feed was fed in a manner that is sprayed throughout the culture tank. On the other hand, commercially grown shrimp, which had been fed by feeding for 16 weeks, containing no turmeric and stevia mixture, were used as a control, and the development of the control and control groups was compared.

TABLE 3

As a result, (i) weight gain in the experimental group was increased by 30% compared to the control group. Specifically, the average weight of the experimental group (26.5g) was significantly higher than the average weight of the control group (20g), the number of animals per 1kg was 40 animals, 50 controls. (ii) The survival rate was also measured at 90%, confirming that it was significantly higher than the survival rate (30%) of the control.

Therefore, the results showed that the weight gain and survival rate of the subjects fed the feed containing the feed additive containing turmeric and stevia mixture was significantly higher than that of the control fed the feed containing no turmeric and stevia mixture. It was excellent. This supports the good growth promoting effect of feed additives containing turmeric and stevia mixture.

실험예 3: 울금 및 스테비아 혼합물이 포함된 사료첨가제를 함유한 사료를 급이한 양식 새우의 성분 변화Experimental Example 3: Changes in Components of Cultured Shrimp Feeded with Feed Additives Containing Turmeric and Stevia Mixtures

In order to examine the change in the composition of the shrimp by the addition of the feed containing the feed additive containing turmeric and stevia, (i) the blending ratio of Preparation Example 2 containing the feed additive containing the turmeric and stevia mixture ( 2 wt.%) Of diets fed with 100 days of feeding, and (ii) water of shrimp cultures of the normal festively-cultivated controls fed 100 days of feed containing no turmeric and stevia mixture. Content, crude ash, crude fat and crude protein content and calories were evaluated, and the results are shown in Table 4 below.

Table 4

As a result, (i) the moisture content was slightly higher in the control than in the control, while (ii) crude ash and crude protein were slightly higher in the control than in the control. (iii) In the case of crude fat, the experimental group (0.51%) decreased by 2 times than the control group (1.05%), and (iv) in the calories, the experimental group (828.4 cal / g) than the control group (791.6 cal / g). It was confirmed that the increase of), the turmeric and stevia mixture of the present invention suggests that it can provide a feed for farming high-performance shrimp as well as growth.

실험예 4: 울금 및 스테비아 혼합물이 포함된 사료첨가제를 함유한 사료 조성물을 급이한 양식장 수질 개선효과Experimental Example 4: Water quality improvement effect of feed composition containing feed additive containing turmeric and stevia mixture

In order to examine the effect of improving the quality of aquaculture farms by the addition of feed containing feed additives containing turmeric and stevia, (i) blending ratio of Preparation Example 2 containing feed additives containing turmeric and stevia mixture ( Aquaculture water (experimental) fed a diet containing 2% by weight) and (ii) aquaculture water (control) fed a feed containing a feed additive that does not contain turmeric and stevia Chemical oxygen demand (COD), the amount of suspended solids, total nitrogen, nitrite nitrogen was compared and the results are shown in Table 5 below.

Table 5

The results showed that (i) the chemical oxygen demand (COD) was about two times lower than the control (9.3 mg / L) and the control (19.5 mg / L), and (ii) suspension, the leading culprit of water deterioration. In the case of the substance, the value of more than 9-fold decrease in the experimental group (7.6 mg / L) compared to the control (60.4 mg / L) was measured. In addition, (iii) the total nitrogen content was also lower in the experimental group (1.64 mg / L) than the control group (6.48 mg / L), and (iv) the nitrite nitrogen content was also lower in the experimental group (0.20 mg / L) than the control group (3.19 mg / L). / L) was measured lower. This suggests that the turmeric and stevia mixture of the present invention is excellent in improving the water environment of the cultured water.

In addition, these results indicate that the use of feed additives containing turmeric and stevia mixtures in aquaculture can provide stable water quality management with little change in the water environment of the aquaculture tanks, as well as economically reducing the number of water retrieval. something to do.

Claims

Shrimp growth, feed additive composition for shrimp growth comprising a mixture of stevia or stevioside as an active ingredient.
The feed additive composition of claim 1, wherein the feed additive has growth promoting and aquaculture water purification functions.
The feed additive composition of claim 1, comprising turmeric and a mixture of stevia or stevioside in a proportion of 0.5 to 3.5% by weight of the total composition.
The feed additive composition of claim 1, wherein the turmeric and stevia mixture is in powder form.
The feed additive composition of claim 1, wherein the turmeric and stevia are present in a mixing ratio of 1: 1 to 1: 3.
A feed comprising the feed additive composition of any one of claims 1 to 5.
A feed additive composition for improving shrimp growth and purifying water quality of shrimp, comprising turmeric and a mixture of stevia or stevioside as an active ingredient.
16. A method of enhancing shrimp growth comprising feeding turmeric and stevia or stevioside to shrimp.
The method of claim 8, wherein the turmeric and stevia are present in a mixing ratio of 1: 1 to 1: 3.
A method of improving the water quality of cultured water comprising administering turmeric and stevia or a mixture of steviosides to shrimp farmed water.
The method of claim 10, wherein the turmeric and stevia are present in a mixing ratio of 1: 1 to 1: 3.