WO2015167310A1 - Feed additive composition containing mixture of turmeric and stevia as active ingredient for promoting growth of anguilla japonica - Google Patents

Feed additive composition containing mixture of turmeric and stevia as active ingredient for promoting growth of anguilla japonica Download PDF

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WO2015167310A1
WO2015167310A1 PCT/KR2015/004450 KR2015004450W WO2015167310A1 WO 2015167310 A1 WO2015167310 A1 WO 2015167310A1 KR 2015004450 W KR2015004450 W KR 2015004450W WO 2015167310 A1 WO2015167310 A1 WO 2015167310A1
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feed
stevia
turmeric
mixture
feed additive
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PCT/KR2015/004450
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French (fr)
Korean (ko)
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이우송
정형재
김영민
노문철
류영배
박수진
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한국생명공학연구원
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    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K10/00Animal feeding-stuffs
    • A23K10/30Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K20/00Accessory food factors for animal feeding-stuffs
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K20/00Accessory food factors for animal feeding-stuffs
    • A23K20/10Organic substances
    • A23K20/163Sugars; Polysaccharides
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K50/00Feeding-stuffs specially adapted for particular animals
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K50/00Feeding-stuffs specially adapted for particular animals
    • A23K50/80Feeding-stuffs specially adapted for particular animals for aquatic animals, e.g. fish, crustaceans or molluscs

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  • the present invention relates to a feed additive composition for growing eel, comprising turmeric and a mixture of stevia or stevioside as an active ingredient, and a feed comprising the same.
  • seafood products such as eel, a representative freshwater fish species
  • eel a representative freshwater fish species
  • the eel is divided into several stages of growth, and it is a species that is difficult to artificially spawn and load.
  • the present inventors have found that by mixing turmeric and stevia or stevioside in a constant ratio and using it as a feed additive, the present inventors confirmed that not only the growth promoting effect of animals, especially eel, but also the effect of purifying the water quality of farmed water can be obtained. Was completed.
  • One object of the present invention is to provide a feed additive composition for eel growth promotion comprising turmeric and a mixture of stevia or stevioside as an active ingredient.
  • Another object of the present invention is to provide a feed comprising the feed additive composition.
  • Still another object of the present invention is to provide a feed additive composition for improving eel growth and purifying water quality of aquaculture comprising turmeric and a mixture of stevia or stevioside as an active ingredient.
  • It is yet another object of the present invention to provide a method for enhancing eel growth comprising feeding turmeric and a mixture of stevia or stevioside to an eel.
  • Another object of the present invention is to provide a method for improving the water quality of cultured water comprising administering turmeric and a mixture of stevia or stevioside to the eel cultured water.
  • the mixture of turmeric and stevia or stevioside of the present invention is included in the feed additive to significantly increase the growth of the eel, and at the same time has the water purification function of the eel cultured water, so it can reduce the mortality due to water deterioration It can be used more usefully in industry.
  • 1 is a view showing the results of the weight increase of the eels by the addition of feed containing turmeric and stevia or stevioside as an active ingredient.
  • FIG. 2 is a view showing the results of the intake of the feed of the eel by the addition of the feed containing turmeric and stevia or stevioside as an active ingredient.
  • Figure 3 is a view showing the results of examining the feed efficiency by the addition of feed containing turmeric and stevia or stevioside mixture as an active ingredient.
  • Figure 4 is a diagram showing the results of examining the feed conversion coefficient by the addition of feed containing turmeric and stevia or stevioside mixture as an active ingredient.
  • FIG. 5 is a diagram showing the pH change of the culture water of the control group fed the feed containing the mixture of turmeric and stevia or stevioside as an active ingredient to the eel for 78 days and the feed not containing the mixture .
  • Figure 6 is a photograph comparing the number of cultures of the control group fed the feed containing the mixture of turmeric and stevia or stevioside as an active ingredient to the eel for 78 days and the feed not containing the mixture.
  • the present invention provides a feed additive composition for promoting eel growth comprising turmeric and a mixture of stevia or stevioside as an active ingredient.
  • “Turmeric” is a perennial herbaceous plant belonging to the genus Ginger and Cucumber, and can be used in various ways for medicinal, edible, dyed, or ornamental.
  • Specific examples of turmeric include: Curcuma wenyujin , YH Chen et C. Ling, Curcuma longa Linne, Curcuma longa Salisb., Curcuma zedoaria , Curcuma zedoaria ( Curcuma kwansiensis , SG Lee et CF Liang), acu ( curcuma aeruginosa ), bonsai ( Curcuma phaeocaulis Val.) Curcuma domestica , but there are limitations no.
  • turmeric may be purchased and used commercially, or may be used collected or grown in nature.
  • the use part may be mainly used as a root part, but is not limited thereto.
  • the dried dried turmeric, or dried bark turmeric dried by removing the bark thereof and dried may be used in powder form.
  • Stevia stevia rebaudiana Bertoni
  • Stevia leaves also contain large amounts of sweet flavoring ingredients, such as stevioside and rebaudioside, which can be used as natural sweeteners.
  • stevia may be purchased and used commercially, or may be used collected or grown in nature. It is preferable to use a leaf part mainly as a use part, but is not limited thereto.
  • stevia may be used as it is, or dried form as it is dried, or dried stevia dried by steaming it may be used in powder form.
  • stevioside is a natural polysaccharide material mainly contained in stevia leaves, specifically, steviosides A1, A2, steviol 1,2,3, dulcoside, rebaudioside a and various forms of sugars such as b, c, e, and f.
  • the stevioside of the present invention may be purchased and used commercially, or may be used extracted and separated from stevia collected or grown in nature. At this time, a well-known method can be used for the extraction and separation method of stevioside.
  • the feed additive composition of the present invention may include turmeric and a mixture of stevia or stevioside as an active ingredient for promoting growth of eel, and the mixture may be present in powder form.
  • the feed additive composition of the present invention may be prepared by adding turmeric and a mixture of stevia or stevioside in an appropriate effective concentration range according to various preparation methods known in the art.
  • the feed additive composition of the present invention may include turmeric and a mixture of stevia or stevioside in a ratio of 0.01 to 100% by weight of the total feed additive composition, but is not limited thereto.
  • the mixing ratio thereof may be arbitrarily selected, but preferably 1:10 to 10: 1, more preferably 1: 1 to 1: 3. Can be mixed in proportions.
  • the mixing ratio thereof may be arbitrarily selected, preferably 1: 100 to 100: 1, more preferably 1:10 to 10. Can be mixed in a ratio of 1: 1.
  • a feed additive composition in which turmeric and stevia powder were mixed at a weight ratio of 3: 7 was prepared and used, and a feed containing a feed additive containing a mixture of turmeric and stevia in the mixed ratio had acute toxicity.
  • Example 1 by significantly increasing the feed intake and weight gain, including the symbol of the feed was confirmed the growth promoting effect of the eel fed the feed (Experimental Example 2).
  • Feed additive composition of the present invention can improve the water quality of cultured water by including turmeric and a mixture of stevia or stevioside as an active ingredient.
  • the feed additive composition in which turmeric and stevia powder were mixed at a weight ratio of 3: 7 was prepared and used, and the feed was added to the feed additive containing the mixture of turmeric and stevia in the mixing ratio. It was confirmed that the suspended solids, chemical oxygen demand and total nitrogen in the aquaculture tank were derived at a significantly lower value than the feed water containing the mixture (Experimental Example 4).
  • Feed additive composition of the present invention may further include a substance added to the feed for the purpose of various effects, such as nutritional supplementation and weight loss prevention, improving meat quality, preventing reproductive disorders, improving conception rate, summer heat stress prevention.
  • the feed additive of the present invention corresponds to a feed supplement in the Feed Control Act, and includes mineral preparations such as sodium bicarbonate, bentonite, magnesium oxide, and composite minerals; Mineral preparations which are trace minerals such as zinc, copper, cobalt and selenium; Vitamin agents such as keratin, vitamins A, D, E, nicotinic acid, and vitamin B complexes; Protective amino acids such as methionine and lyric acid; Protective fatty acids such as fatty acid calcium salts; Probiotics, yeasts such as probiotics (lactic acid bacteria), yeast cultures, mold fermentation products; And the like may further be included.
  • the feed additive composition of the present invention and the feed containing the same are plant protein feeds such as wheat, barley, corn, ground or crushed as auxiliary ingredients, animal protein feeds such as blood meal, meat meal, fish meal, animal fat and vegetable fat
  • plant protein feeds such as wheat, barley, corn, ground or crushed as auxiliary ingredients
  • animal protein feeds such as blood meal, meat meal, fish meal, animal fat and vegetable fat
  • it can be used with nutritional supplements, growth promoters, digestive absorption accelerators and disease prevention agents.
  • the feed additive composition of the present invention can be applied to a number of animal diets, ie feed, including mammals, poultry, fish and shellfish.
  • Animal feed blending method comprising a feed additive composition according to the present invention
  • the feed additive composition of the present invention can be mixed with animal feed in an amount of about 1g to 500g, preferably 10g to 100g per 1kg on a dry weight basis have.
  • the feed mixture may be thoroughly mixed and then fed into a mesh or subjected to a palletizing, expanding and extruding process through a further processing process.
  • the present invention provides a feed comprising the feed additive composition.
  • the feed additive composition may be included in the feed to not only enhance the growth of the eel, but also improve the quality of the cultured water.
  • feed includes any natural or artificial diet, meal, etc. for or suitable for the animal to eat, ingest and digest.
  • Feed containing a feed additive containing turmeric and a mixture of stevia or stevioside of the present invention as an active ingredient can be prepared from various types of feed known in the art, preferably thick feed, feed and / or special Feed may be included.
  • the feed of the present invention may include the feed additive composition in a ratio of 0.01 to 10.0% by weight of the total feed, preferably may be included in a ratio of 0.3 to 0.5% by weight.
  • turmeric and a mixture of stevia or stevioside may be included in a ratio of 0.001 to 5.0% by weight of the total feed, preferably 0.1 to 0.5% by weight, but is not limited thereto.
  • the present invention provides a method for enhancing eel growth comprising feeding the feed additive composition, a feed or turmeric containing the same, and a mixture of stevia or stevioside to the eel.
  • the present invention also provides a method for improving the water quality of the cultured water comprising administering the feed additive composition, a feed or turmeric containing the same, and a mixture of stevia or stevioside to the eel cultured water.
  • the turmeric and stevia may be present in a mixing ratio of 1: 1 to 1: 3.
  • Turmeric and stevia were washed and dried, and then ground to prepare a powder having a particle size of 50 to 100 mesh. Each prepared powder was mixed with a turmeric: stevia ratio of 3: 7 to prepare a mixture.
  • the feed additive prepared in Preparation Example 1 was mixed in the mixing ratio of Table 2 to prepare a feed. This was then used as a sample of the experimental example.
  • young eel (8-12 cm) was cultured for 10 days in an aquarium (120 ⁇ 40 ⁇ 40 cm) maintained at a water temperature of 27 ⁇ 2 ° C., and the eel was purified for 1 week before being used in the experiment.
  • the number of eel used in the experiment was 30 per aquarium, and the feed of the compounding ratio (0.5 to 10% by weight of the feed additive composition) of Examples 1 to 3 was administered twice a day 20g twice, as a control. Only normal feeds containing no turmeric and stevia mixtures were fed.
  • the tank for eel farming is eel in an 18m ⁇ 18m ⁇ 1.7m sized aquarium in a greenhouse equipped with a shading screen to maintain automatic temperature (28 ⁇ 1.0 °C) control, oxygen supply, direct sunlight shading, and a quiet environment.
  • the control group showed a total of 35,938 tailings of 21g of fish in the average initiator, and the experimental group of 18,452 tailings and 20,804 tailings of 35.7g of fish in the average initiator, respectively.
  • the feeder was mounted just below the surface of water in a suitable size to the device attached to the wall of each aquaculture tank, and the feed was fed by making it into a dough form using a feed maker.
  • Table 4 The results of breeding eel in each cultured tank for 78 days when the experimental diet was administered are shown in Table 4 below.
  • Table 5 Item Control Feed Additive Ratio (wt%) 0.3 0.5 morning afternoon morning afternoon morning afternoon Max (minutes) 8.40 9.30 5.40 4.50 6.00 4.50 Min (minutes) 2.20 2.30 2.00 1.30 1.50 1.55 Average minutes 3.76 3.76 2.63 2.45 2.60 2.62
  • the average intake time (2.45 to 2.63 minutes) of the experimental group was shorter than the average intake time (3.76 minutes) of the control group was confirmed that the feed was ingested faster in the experimental group than the control group. This suggests that the food acceptability of eel increased due to the addition of feed additives containing turmeric and stevia mixtures.
  • the average weight (400g) of the experimental group was significantly higher than the average weight (230g) of the control, the number of animals per 1kg was 2.5 animals and 4.5 control animals. This supports the good growth promoting effect of feed additives containing turmeric and stevia mixture.
  • Table 8 Item Control Feed additive addition rate (%) 0.3 (Example 4) 0.5 (Example 3) pH at least 4.60 4.77 4.75 maximum 6.30 7.14 7.15 Average 5.39 ⁇ 0.45 5.69 ⁇ 0.60 5.97 ⁇ 0.46 Coefficient of variation (%) 8.28 10.56 7.76 DO (ppm) at least 6.3 6.0 6.1 maximum 7.5 7.7 7.2 Average 6.8 ⁇ 0.31 6.6 ⁇ 0.50 6.45 ⁇ 0.30 Coefficient of variation (%) 4.55 7.6 4.71 NO 2 -N (ppm) at least One One One maximum 10 10 10 Average 4.49 ⁇ 0.88 4.0 ⁇ 0.28 3.8 ⁇ 0.54 Coefficient of variation (%) 19.7 28.4 54.2 NH 3 -N (ppm) at least 3 One One maximum 12 10 4 Average 9.9 ⁇ 1.74 5.38 ⁇ 2.30 2.52 ⁇ 1.13 Coefficient of variation (%) 17.6 42.7 44.9

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Abstract

The present invention relates to a feed additive composition for promoting the growth of Anguilla japonica and a feed including same, the feed additive composition including a mixture of turmeric and stevia or stevioside as an active ingredient. The mixture of the present invention is included in the feed additive, thereby significantly promoting the growth of Anguilla japonica and simultaneously showing a water quality purifying function of purifying water for Anguilla japonica aquaculture, so that it is possible to reduce a mortality rate due to water quality deterioration. Thus, the mixture of the present invention can be more useful in the Anguilla japonica aquaculture industry.

Description

울금 및 스테비아의 혼합물을 유효성분으로 포함하는 뱀장어 생육증진용 사료첨가제 조성물Feed additive composition for growth of eel, comprising a mixture of turmeric and stevia as an active ingredient
본 발명은 울금 및, 스테비아 또는 스테비오사이드의 혼합물을 유효성분으로 포함하는 뱀장어 생육증진용 사료첨가제 조성물 및 이를 포함하는 사료에 관한 것이다.The present invention relates to a feed additive composition for growing eel, comprising turmeric and a mixture of stevia or stevioside as an active ingredient, and a feed comprising the same.
근래의 어류양식 산업은 사육환경의 악화와 수질환경의 오염으로 인한 스트레스의 가중으로 인하여 어체의 활성이 저하되고 성장저하, 육질저하, 및 항생제의 오남용에 직면해 있다. 특히, 항생제의 오남용으로 인한 어체 내 축적, 항생제 매성균주의 확산, 및 수질오염이 연쇄적으로 문제되는 바, 현 시점에서 천연원료를 이용한 자연면역력 증강 및 질병예방에 관한 새로운 대체 첨가제 개발은 반드시 필요하다.In recent years, the fish farming industry suffers from the deterioration of the farming environment and the increase of stress caused by the pollution of the water environment, which leads to a decrease in the activity of the fish, a decrease in the meat quality, and a misuse of antibiotics. In particular, the accumulation of antibiotics in the body due to misuse of antibiotics, the proliferation of antibiotics, and water pollution are a series of problems. At this point, development of new alternative additives for enhancing natural immunity and preventing diseases using natural ingredients is essential. Do.
한편, 대표적인 민물 양식어종인 뱀장어 등의 수산식품은 국민 소득 증대와 건강에 대한 관심으로 나날이 질적, 양적 증대가 이루어지고 있다. 특히 뱀장어는 성장단계가 여러 단계가 나누어져 있고, 인공산란 및 부하가 어려운 어종으로서, 성장 중간단계인 실뱀장어(시라시)를 자연 채취하여 적당한 환경에서 양식을 해야 하는 현실임에도, 지구 온난화에 따른 실뱀장어의 어획량이 줄어들면서 가격의 상승과 공급부족으로 인한 국내 양식장 중 10∼15%(50곳)만 실뱀장어를 입식하여 양식을 하고 있는 실정이다. 또한 내수면 양식에서 뱀장어 양식 비중이 높게 나타나고 있음에도 불구하고, 아직까지 뱀장어 전용 양식 사료첨가제에 대한 개발은 전혀 이루어지지 않고 있다.On the other hand, seafood products such as eel, a representative freshwater fish species, are increasing in quality and quantity day by day due to the increase in national income and health. In particular, the eel is divided into several stages of growth, and it is a species that is difficult to artificially spawn and load. As the catches of the eel have been reduced, only 10-15% (50 places) of domestic farms are stocked by the eel due to the price increase and the shortage of supply. In addition, despite the high proportion of eel farming in the inland water culture, there has been no development of eel farm feed additives.
이러한 점을 살펴볼 때, 고기능성 사료첨가제 또는 수입 사료보다 우수한 사료효율을 가지면서 질병을 예방할 수 있는 뱀장어용 양식 사료의 개발이 매우 필요한 실정이다.Considering this point, it is very necessary to develop a cultured feed for eel that can prevent disease while having a higher feed efficiency than a high-performance feed additive or imported feed.
본 발명자는 울금 및, 스테비아 또는 스테비오사이드를 일정한 비율로 혼합하여 사료첨가제로서 사용함으로써 동물, 특히 뱀장어의 생육증진 효과뿐만 아니라, 양식수의 수질을 정화하는 효과를 동시에 나타낼 수 있음을 확인하여 본 발명을 완성하였다.The present inventors have found that by mixing turmeric and stevia or stevioside in a constant ratio and using it as a feed additive, the present inventors confirmed that not only the growth promoting effect of animals, especially eel, but also the effect of purifying the water quality of farmed water can be obtained. Was completed.
본 발명의 하나의 목적은 울금 및, 스테비아 또는 스테비오사이드의 혼합물을 유효성분으로 포함하는 뱀장어 생육증진용 사료첨가제 조성물을 제공하는 것이다.One object of the present invention is to provide a feed additive composition for eel growth promotion comprising turmeric and a mixture of stevia or stevioside as an active ingredient.
본 발명의 다른 목적은 상기 사료첨가제 조성물을 포함하는 사료를 제공하는 것이다.Another object of the present invention is to provide a feed comprising the feed additive composition.
본 발명의 또 다른 목적은 울금 및, 스테비아 또는 스테비오사이드의 혼합물을 유효성분으로 포함하는 뱀장어 생육증진용 및 양식수 수질 정화용 사료첨가제 조성물을 제공하는 것이다.Still another object of the present invention is to provide a feed additive composition for improving eel growth and purifying water quality of aquaculture comprising turmeric and a mixture of stevia or stevioside as an active ingredient.
본 발명의 또 다른 목적은 울금 및, 스테비아 또는 스테비오사이드의 혼합물을 뱀장어에 급이하는 단계를 포함하는 뱀장어 생육을 증진시키는 방법을 제공하는 것이다.It is yet another object of the present invention to provide a method for enhancing eel growth comprising feeding turmeric and a mixture of stevia or stevioside to an eel.
본 발명의 또 다른 목적은 울금 및, 스테비아 또는 스테비오사이드의 혼합물을 뱀장어 양식수에 투여하는 단계를 포함하는 양식수의 수질을 개선시키는 방법을 제공하는 것이다.Another object of the present invention is to provide a method for improving the water quality of cultured water comprising administering turmeric and a mixture of stevia or stevioside to the eel cultured water.
본 발명의 울금 및 스테비아 또는 스테비오사이드의 혼합물은 사료첨가제에 포함되어 뱀장어의 생육을 현저히 증진시킴과 동시에, 뱀장어 양식수의 수질 정화기능을 가지고 있어, 수질악화로 인한 폐사율을 감소시킬 수 있으므로 뱀장어 양식산업에 보다 유용하게 사용될 수 있다.The mixture of turmeric and stevia or stevioside of the present invention is included in the feed additive to significantly increase the growth of the eel, and at the same time has the water purification function of the eel cultured water, so it can reduce the mortality due to water deterioration It can be used more usefully in industry.
도 1은 울금 및 스테비아 또는 스테비오사이드의 혼합물을 유효성분으로 포함하는 사료의 첨가에 의한 뱀장어의 증체량을 조사한 결과를 나타내는 도이다.1 is a view showing the results of the weight increase of the eels by the addition of feed containing turmeric and stevia or stevioside as an active ingredient.
도 2는 울금 및 스테비아 또는 스테비오사이드의 혼합물을 유효성분으로 포함하는 사료의 첨가에 의한 뱀장어의 사료의 섭취량을 조사한 결과를 나타내는 도이다.2 is a view showing the results of the intake of the feed of the eel by the addition of the feed containing turmeric and stevia or stevioside as an active ingredient.
도 3은 울금 및 스테비아 또는 스테비오사이드의 혼합물을 유효성분으로 포함하는 사료의 첨가에 의한 사료효율을 조사한 결과를 나타내는 도이다.Figure 3 is a view showing the results of examining the feed efficiency by the addition of feed containing turmeric and stevia or stevioside mixture as an active ingredient.
도 4는 울금 및 스테비아 또는 스테비오사이드의 혼합물을 유효성분으로 포함하는 사료의 첨가에 의한 사료 전환계수를 조사한 결과를 나타내는 도이다.Figure 4 is a diagram showing the results of examining the feed conversion coefficient by the addition of feed containing turmeric and stevia or stevioside mixture as an active ingredient.
도 5는 울금 및 스테비아 또는 스테비오사이드의 혼합물을 유효성분으로 포함하는 사료를 뱀장어에 78일간 급이한 실험구와 상기 혼합물을 포함하지 않은 사료를 급이한 대조구의 양식수의 pH 변화를 나타낸 도이다.5 is a diagram showing the pH change of the culture water of the control group fed the feed containing the mixture of turmeric and stevia or stevioside as an active ingredient to the eel for 78 days and the feed not containing the mixture .
도 6은 울금 및 스테비아 또는 스테비오사이드의 혼합물을 유효성분으로 포함하는 사료를 뱀장어에 78일간 급이한 실험구와 상기 혼합물을 포함하지 않은 사료를 급이한 대조구의 양식수를 비교한 사진이다.Figure 6 is a photograph comparing the number of cultures of the control group fed the feed containing the mixture of turmeric and stevia or stevioside as an active ingredient to the eel for 78 days and the feed not containing the mixture.
상기 과제를 해결하기 위한 하나의 양태로서, 본 발명은 울금 및, 스테비아 또는 스테비오사이드의 혼합물을 유효성분으로 포함하는 뱀장어 생육증진용 사료첨가제 조성물을 제공한다.As one embodiment for solving the above problems, the present invention provides a feed additive composition for promoting eel growth comprising turmeric and a mixture of stevia or stevioside as an active ingredient.
본 발명에서 "울금(Turmeric)"은, 생강과 커규마 속에 속하는 다년생 초본식물로서 약용, 식용, 염색용, 또는 관상용으로 다양하게 사용될 수 있다. 울금의 구체적 예로는, 온울금(Curcuma wenyujin, Y. H. Chen et C. Ling), 가을울금(황울금)(Curcuma longa Linne), 봄울금(Curcuma longa Salisb.), 보라색울금(Curcuma zedoaria), 광서아출(계울금)(Curcuma kwansiensis, S. G. Lee et C. F. Liang), 아출(녹사울금)(Curcuma aeruginosa), 봉아출(Curcuma phaeocaulis Val.) 커큐마 도메스티카(Curcuma domestica) 등이 있으나, 이에 제한되는 것은 아니다. 본 발명에서 울금은 상업적으로 판매되는 것을 구입하여 사용하거나, 자연에서 채취 또는 재배된 것을 사용할 수 있다. 사용부위로는 주로 뿌리 부위를 사용할 수 있으나, 이에 제한되지 않는다. 또한 그대로 말려 건조된 형태, 또는 이의 주피를 제거하고 쪄서 말린 건조된 울금은 분말화하여 분말 형태로 사용할 수도 있다. In the present invention, "Turmeric" is a perennial herbaceous plant belonging to the genus Ginger and Cucumber, and can be used in various ways for medicinal, edible, dyed, or ornamental. Specific examples of turmeric include: Curcuma wenyujin , YH Chen et C. Ling, Curcuma longa Linne, Curcuma longa Salisb., Curcuma zedoaria , Curcuma zedoaria ( Curcuma kwansiensis , SG Lee et CF Liang), acu ( curcuma aeruginosa ), bonsai ( Curcuma phaeocaulis Val.) Curcuma domestica , but there are limitations no. In the present invention, turmeric may be purchased and used commercially, or may be used collected or grown in nature. The use part may be mainly used as a root part, but is not limited thereto. In addition, the dried dried turmeric, or dried bark turmeric dried by removing the bark thereof and dried may be used in powder form.
본 발명에서 "스테비아(Stevia rebaudiana Bertoni)"는, 국화과에 속하는 다년초 여러해살이풀로서 허브차, 음료, 한약조제, 건강보조식품 등에 사용될 수 있다. 또한 스테비아 잎에는 스테비오사이드(stevioside)와 리바우디오사이드(rebaudioside)와 같은 단맛을 내는 성분을 다량 포함하고 있어, 천연감미료로도 사용될 수 있다. 본 발명에서 스테비아는 상업적으로 판매되는 것을 구입하여 사용하거나, 자연에서 채취 또는 재배된 것을 사용할 수 있다. 사용부위로는 주로 잎 부위를 사용하는 것이 바람직하나, 이에 제한되는 것은 아니다. 본 발명에서 스테비아는 그대로 사용하거나, 또는 이를 그대로 말린 건조된 형태, 또는 이를 쪄서 말린 건조된 스테비아는 분말화하여 분말 형태로 사용할 수도 있다.In the present invention, "Stevia ( stevia rebaudiana Bertoni ), perennial perennial herb belonging to the Asteraceae can be used in herbal tea, beverages, herbal preparations, health supplements and the like. Stevia leaves also contain large amounts of sweet flavoring ingredients, such as stevioside and rebaudioside, which can be used as natural sweeteners. In the present invention, stevia may be purchased and used commercially, or may be used collected or grown in nature. It is preferable to use a leaf part mainly as a use part, but is not limited thereto. In the present invention, stevia may be used as it is, or dried form as it is dried, or dried stevia dried by steaming it may be used in powder form.
본 발명에서 "스테비오사이드(stevioside)"는 스테비아 잎에 주로 함유되어 있는 천연 다당류 물질로서, 구체적으로 스테비오사이드 A1, A2, 스테비올 1,2,3, 둘코사이드, 리바우디오사이드(Rebaudioside) a,b,c,e,f 등 다양한 형태의 당을 포함한다. 본 발명의 스테비오사이드는 상업적으로 판매되는 것을 구입하여 사용하거나, 자연에서 채취 또는 재배된 스테비아로부터 추출·분리된 것을 사용할 수 있다. 이때 스테비오사이드의 추출·분리 방법은 공지된 방법을 사용할 수 있다.In the present invention, "stevioside" is a natural polysaccharide material mainly contained in stevia leaves, specifically, steviosides A1, A2, steviol 1,2,3, dulcoside, rebaudioside a and various forms of sugars such as b, c, e, and f. The stevioside of the present invention may be purchased and used commercially, or may be used extracted and separated from stevia collected or grown in nature. At this time, a well-known method can be used for the extraction and separation method of stevioside.
본 발명의 사료첨가제 조성물은 뱀장어의 생육증진을 위하여 울금 및, 스테비아 또는 스테비오사이드의 혼합물을 유효성분으로 포함할 수 있으며, 상기 혼합물은 분말 형태로 존재할 수 있다.The feed additive composition of the present invention may include turmeric and a mixture of stevia or stevioside as an active ingredient for promoting growth of eel, and the mixture may be present in powder form.
본 발명의 사료첨가제 조성물은 당업계에 공지된 다양한 제조방법에 따라 적절한 유효 농도 범위에서 울금 및, 스테비아 또는 스테비오사이드의 혼합물을 첨가하여 제조 가능하다. 구체적으로 본 발명의 사료첨가제 조성물은 울금 및, 스테비아 또는 스테비오사이드의 혼합물을 전체 사료첨가제 조성물 중 0.01 내지 100중량%의 비율로 포함할 수 있으나, 이에 제한되지 않는다. The feed additive composition of the present invention may be prepared by adding turmeric and a mixture of stevia or stevioside in an appropriate effective concentration range according to various preparation methods known in the art. Specifically, the feed additive composition of the present invention may include turmeric and a mixture of stevia or stevioside in a ratio of 0.01 to 100% by weight of the total feed additive composition, but is not limited thereto.
상기 사료첨가제 조성물의 유효성분으로 울금 및 스테비아 혼합물을 포함하는 경우, 이들의 혼합 비율은 임의로 선택할 수 있으나, 바람직하게는 1:10 내지 10:1, 보다 바람직하기로는 1:1 내지 1:3의 비율로 혼합될 수 있다.When the turmeric and stevia mixture is included as an active ingredient of the feed additive composition, the mixing ratio thereof may be arbitrarily selected, but preferably 1:10 to 10: 1, more preferably 1: 1 to 1: 3. Can be mixed in proportions.
또한, 상기 사료첨가제 조성물의 유효성분으로 울금 및 스테비오사이드 혼합물을 포함하는 경우, 이들의 혼합 비율은 임의로 선택할 수 있으나, 바람직하게는 1:100 내지 100:1, 보다 바람직하기로는 1:10 내지 10:1의 비율로 혼합될 수 있다.In addition, when the turmeric and stevioside mixture is included as an active ingredient of the feed additive composition, the mixing ratio thereof may be arbitrarily selected, preferably 1: 100 to 100: 1, more preferably 1:10 to 10. Can be mixed in a ratio of 1: 1.
본 발명의 일 실험예에서는 울금 및 스테비아 분말이 3:7의 중량비로 혼합된 사료첨가제 조성물을 제조 및 사용하였으며, 상기 혼합비의 울금 및 스테비아의 혼합물을 포함하는 사료첨가제를 첨가한 사료가 급성 독성을 나타내지 않으면서도(실험예 1), 사료기호도를 비롯하여 사료섭취량 및 증체량을 현저히 증가시킴으로써 상기 사료를 섭취한 뱀장어의 생육증진 효과를 확인하였다(실험예 2). In an experimental example of the present invention, a feed additive composition in which turmeric and stevia powder were mixed at a weight ratio of 3: 7 was prepared and used, and a feed containing a feed additive containing a mixture of turmeric and stevia in the mixed ratio had acute toxicity. Although not shown (Experimental Example 1), by significantly increasing the feed intake and weight gain, including the symbol of the feed was confirmed the growth promoting effect of the eel fed the feed (Experimental Example 2).
본 발명의 사료첨가제 조성물은 울금 및, 스테비아 또는 스테비오사이드의 혼합물을 유효성분으로 포함함으로써 양식수의 수질을 개선시킬 수 있다. Feed additive composition of the present invention can improve the water quality of cultured water by including turmeric and a mixture of stevia or stevioside as an active ingredient.
본 발명의 일 실험예에서는 울금 및 스테비아 분말이 3:7의 중량비로 혼합된 사료첨가제 조성물을 제조 및 사용하였으며, 상기 혼합비의 울금 및 스테비아의 혼합물을 포함하는 사료첨가제를 첨가한 사료를 급이한 양식조에서 부유물질, 화학적 산소요구량 및 총 질소량이 상기 혼합물을 포함하지 않은 사료를 급이한 양식수보다 현저히 낮은 수치로 도출되었음을 확인하였다(실험예 4). In an experimental example of the present invention, the feed additive composition in which turmeric and stevia powder were mixed at a weight ratio of 3: 7 was prepared and used, and the feed was added to the feed additive containing the mixture of turmeric and stevia in the mixing ratio. It was confirmed that the suspended solids, chemical oxygen demand and total nitrogen in the aquaculture tank were derived at a significantly lower value than the feed water containing the mixture (Experimental Example 4).
따라서 울금 및 스테비아의 혼합물의 탁월한 양식수 수질환경 개선효과를 토대로, 양식어장의 사육환경 중 가장 중요한 환경 요소인 양식조 수질오염과 수중 용존 산소량을 고려하지 않고 개발된 기존 양식 사료의 문제점을 동시에 개선할 수 있다. Therefore, based on the excellent water quality improvement effect of the mixture of turmeric and stevia, the problems of the existing aquaculture feed developed without considering the water pollution and the dissolved oxygen in the water, which are the most important environmental factors in the farming environment, are simultaneously improved. can do.
본 발명의 사료첨가제 조성물은 영양소 보충 및 체중감소 예방, 육질 개선, 번식장애 예방, 수태율 향상, 하절기 고온 스트레스 예방 등 다양한 효과를 목적으로 사료에 첨가하는 물질을 추가로 포함할 수 있다. 본 발명의 사료첨가제는 사료관리법상의 보조사료에 해당하며, 탄산수소나트륨, 벤토나이트(bentonite), 산화마그네슘, 복합광물질 등의 광물질제제; 아연, 구리, 코발트, 셀레늄 등의 미량 광물질인 미네랄제제; 케로틴, 비타민 A, D, E, 니코틴산, 비타민 B 복합체 등의 비타민제; 메티오닌, 리이산 등의 보호아미노산제; 지방산 칼슘염 등의 보호지방산제; 생균제(유산균제), 효모배양물, 곰팡이 발효물 등의 생균, 효모제; 등이 추가로 포함될 수 있다. 또한, 본 발명의 사료첨가제 조성물 및 이를 포함하는 사료는 보조성분으로 분쇄 또는 파쇄된 밀, 보리, 옥수수 등의 식물성 단백질사료, 혈분, 육분, 생선분 등의 동물성 단백질 사료, 동물성 지방 및 식물성 지방 같은 주성분 이외에도 영양 보충제, 성장 촉진제, 소화 흡수 촉진제, 질병 예방제와 함께 사용될 수 있다.Feed additive composition of the present invention may further include a substance added to the feed for the purpose of various effects, such as nutritional supplementation and weight loss prevention, improving meat quality, preventing reproductive disorders, improving conception rate, summer heat stress prevention. The feed additive of the present invention corresponds to a feed supplement in the Feed Control Act, and includes mineral preparations such as sodium bicarbonate, bentonite, magnesium oxide, and composite minerals; Mineral preparations which are trace minerals such as zinc, copper, cobalt and selenium; Vitamin agents such as keratin, vitamins A, D, E, nicotinic acid, and vitamin B complexes; Protective amino acids such as methionine and lyric acid; Protective fatty acids such as fatty acid calcium salts; Probiotics, yeasts such as probiotics (lactic acid bacteria), yeast cultures, mold fermentation products; And the like may further be included. In addition, the feed additive composition of the present invention and the feed containing the same are plant protein feeds such as wheat, barley, corn, ground or crushed as auxiliary ingredients, animal protein feeds such as blood meal, meat meal, fish meal, animal fat and vegetable fat In addition to the active ingredient, it can be used with nutritional supplements, growth promoters, digestive absorption accelerators and disease prevention agents.
본 발명의 사료첨가제 조성물은 포유류, 가금류, 어패류 및 갑각류를 포함하는 다수의 동물 식이 즉, 사료에 적용할 수 있다. The feed additive composition of the present invention can be applied to a number of animal diets, ie feed, including mammals, poultry, fish and shellfish.
본 발명에 따른 사료첨가제 조성물을 포함하는 동물용 사료 배합 방법은, 본 발명의 사료첨가제 조성물을 동물사료에 건조 중량 기준으로 1kg 당 약 1g 내지 500g, 바람직하기로는 10g 내지 100g의 양으로 혼합할 수 있다. 또한 사료 혼합물을 완전히 혼합한 후, 매쉬로 공급하거나, 추가가공 공정을 통하여 팰렛화, 팽창화, 압출 공정을 거칠 수 있다.Animal feed blending method comprising a feed additive composition according to the present invention, the feed additive composition of the present invention can be mixed with animal feed in an amount of about 1g to 500g, preferably 10g to 100g per 1kg on a dry weight basis have. In addition, the feed mixture may be thoroughly mixed and then fed into a mesh or subjected to a palletizing, expanding and extruding process through a further processing process.
또 다른 양태로서, 본 발명은 상기 사료첨가제 조성물을 포함하는 사료를 제공한다. 상기 사료첨가제 조성물이 사료에 포함됨으로써 뱀장어의 생육을 증진시킬 수 있을 뿐만 아니라, 양식수 수질을 개선시킬 수도 있다.In another aspect, the present invention provides a feed comprising the feed additive composition. The feed additive composition may be included in the feed to not only enhance the growth of the eel, but also improve the quality of the cultured water.
본 발명에서 "사료"는 동물이 먹고, 섭취하며, 소화시키기 위한 또는 이에 적당한 임의의 천연 또는 인공 규정식, 한끼식 등을 포함한다. 본 발명의 울금 및, 스테비아 또는 스테비오사이드의 혼합물을 유효성분으로 포함하는 사료첨가제를 함유한 사료는 당업계의 공지된 다양한 형태의 사료로 제조가능하며, 바람직하게는 농후사료, 조사료 및/또는 특수사료가 포함될 수 있다.In the present invention, "feed" includes any natural or artificial diet, meal, etc. for or suitable for the animal to eat, ingest and digest. Feed containing a feed additive containing turmeric and a mixture of stevia or stevioside of the present invention as an active ingredient can be prepared from various types of feed known in the art, preferably thick feed, feed and / or special Feed may be included.
본 발명의 사료는 상기 사료첨가제 조성물을 전체 사료 중 0.01 내지 10.0 중량%의 비율로 포함할 수 있고, 바람직하게는 0.3 내지 0.5 중량%의 비율로 포함할 수 있다. 또한 울금 및, 스테비아 또는 스테비오사이드의 혼합물은 전체 사료 중 0.001 내지 5.0 중량%의 비율로 포함할 수 있고, 바람직하게는 0.1 내지 0.5 중량%의 비율로 포함할 수 있으나, 이에 제한되지 않는다. The feed of the present invention may include the feed additive composition in a ratio of 0.01 to 10.0% by weight of the total feed, preferably may be included in a ratio of 0.3 to 0.5% by weight. In addition, turmeric and a mixture of stevia or stevioside may be included in a ratio of 0.001 to 5.0% by weight of the total feed, preferably 0.1 to 0.5% by weight, but is not limited thereto.
또 다른 양태로서, 본 발명은 상기 사료첨가제 조성물, 이를 포함하는 사료 또는 울금 및, 스테비아 또는 스테비오사이드의 혼합물을 뱀장어에 급이하는 단계를 포함하는 뱀장어 생육을 증진시키는 방법을 제공한다.In another aspect, the present invention provides a method for enhancing eel growth comprising feeding the feed additive composition, a feed or turmeric containing the same, and a mixture of stevia or stevioside to the eel.
또한, 본 발명은 상기 사료첨가제 조성물, 이를 포함하는 사료 또는 울금 및, 스테비아 또는 스테비오사이드의 혼합물을 뱀장어 양식수에 투여하는 단계를 포함하는 양식수의 수질을 개선시키는 방법을 제공한다.The present invention also provides a method for improving the water quality of the cultured water comprising administering the feed additive composition, a feed or turmeric containing the same, and a mixture of stevia or stevioside to the eel cultured water.
상기 울금 및 스테비아는 1 : 1 내지 1 : 3의 혼합비로 존재할 수 있다.The turmeric and stevia may be present in a mixing ratio of 1: 1 to 1: 3.
이하, 실시예를 통하여 본 발명을 더욱 상세히 설명하고자 한다. 이들 실시예는 본 발명을 보다 구체적으로 설명하기 위한 것으로, 본 발명의 범위가 이들 실시예에 의해 제한되는 것은 아니다.Hereinafter, the present invention will be described in more detail with reference to Examples. These examples are intended to illustrate the present invention more specifically, but the scope of the present invention is not limited by these examples.
제조예 1: 울금 및 스테비아의 혼합물(사료첨가제)의 제조Preparation Example 1 Preparation of Mixture of Turmeric and Stevia (Feed Additive)
울금 및 스테비아를 세척하고 건조시킨 후 각각 분쇄하여 50∼100 메쉬의 입도를 가지는 분말을 제조하였다. 각각 제조된 분말을 울금 : 스테비아 비율을 3 : 7 로 혼합하여 혼합물을 제조하였다. Turmeric and stevia were washed and dried, and then ground to prepare a powder having a particle size of 50 to 100 mesh. Each prepared powder was mixed with a turmeric: stevia ratio of 3: 7 to prepare a mixture.
제조예 2: 울금 및 스테비아의 혼합물이 포함된 사료첨가제 조성물의 제조Preparation Example 2 Preparation of a Feed Additive Composition Containing a Mixture of Turmeric and Stevia
상기 제조예 1에서 제조된 울금 및 스테비아 혼합물 0.5 kg을 어분, 전분, 비타민, 미네랄 등으로 이루어진 뱀장어 양식사료에 배합하여 본 발명의 뱀장어 양식용 사료를 제조하였다(표 1).0.5 kg of the turmeric and stevia mixture prepared in Preparation Example 1 were mixed with an eel cultured feed consisting of fish meal, starch, vitamins, minerals, and the like to prepare an eel cultured feed of the present invention (Table 1).
표 1
원료명 배합비율(%) 비고
어분 73.55 카만차카어분 #2,#3,칼데라 어분/브라운 이따따사 어분,러시아화이트어분
알파감자전분 8.00
알파 티피오카 전분 16.00
비타민 프리믹스 1.0 비타민 프리믹스 1Kg중 함량: Vit.A 375 KIU, Vit.D3 750 KIU, Vit.E 4.5g, Vit.K3 75mg, B1 100mg, B2 250mg, B6 164mg, B12 5mg, Biotin 5mg, Folic acid 49mg, Niacin 1g, Ca-Pantothenate 1.029g, Vit. C 9.7g
미네랄 프리믹스 0.5 미네랄 프리믹스 1Kg중 함량: Mg 13g, Mn 6.0g, Cu 1.5g, Fe 20g, Zn 4.5g, I 0.2g, Co 0.35g
일,이인산 칼슘 0.3
염화콜린 0.15
기능성 첨가제 0.5 울금 및 스테비아 혼합물 (3 : 7의 중량비)
100
Table 1
Raw material name Compounding ratio (%) Remarks
Fishmeal 73.55 Kamanchaka Meal # 2, # 3, Caldera Meal / Brown Itatha Meal, Russian White Meal
Alpha Potato Starch 8.00
Alpha Tipioca Starch 16.00
Vitamin Premix 1.0 Content in 1Kg of Vitamin Premix: Vit.A 375 KIU, Vit.D 3 750 KIU, Vit.E 4.5g, Vit.K 3 75mg, B 1 100mg, B 2 250mg, B 6 164mg, B 12 5mg, Biotin 5mg, Folic acid 49 mg, Niacin 1 g, Ca-Pantothenate 1.029 g, Vit. C 9.7 g
Mineral premix 0.5 Content in 1Kg of mineral premix: Mg 13g, Mn 6.0g, Cu 1.5g, Fe 20g, Zn 4.5g, I 0.2g, Co 0.35g
Day, calcium diphosphate 0.3
Choline chloride 0.15
Functional additives 0.5 Turmeric and stevia mixture (weight ratio of 3: 7)
system 100
실시예 1 내지 4: 사료첨가제의 함유량을 달리한 사료 조성물Examples 1 to 4: Feed composition with different content of feed additives
상기 제조예 1에서 제조한 사료첨가제를 하기 표 2의 혼합비율로 혼합하여 사료를 제조하였다. 이를 이후 실험예의 시료로 사용하였다.The feed additive prepared in Preparation Example 1 was mixed in the mixing ratio of Table 2 to prepare a feed. This was then used as a sample of the experimental example.
표 2
구분 울금(중량부) 스테비아(중량부) 사료 및 사료첨가제 배합비(사료/사료첨가제; 중량%)
실시예 1 3 7 95/5
실시예 2 3 7 90/10
실시예 3 3 7 99.5/0.5
실시예 4 3 7 99.7/0.3
TABLE 2
division Turmeric (part by weight) Stevia (part by weight) Combined feed and feed additives (feed / feed additive; weight percent)
Example 1 3 7 95/5
Example 2 3 7 90/10
Example 3 3 7 99.5 / 0.5
Example 4 3 7 99.7 / 0.3
실험예 1: 울금 및 스테비아 혼합물이 포함된 사료첨가제를 함유한 사료 조성물의 급성 독성 실험Experimental Example 1: Acute Toxicity Test of Feed Composition Containing Feed Additives Containing Turmeric and Stevia Mixtures
울금 및 스테비아 혼합물이 포함된 사료첨가제를 함유한 사료 조성물의 급성 독성을 알아보기 위하여, 뱀장어 30마리를 수족관에 투하하고 상기 실시예 1 내지 3의 사료 조성물을 10일 동안 투여하여 독성 평가를 실시하였다. In order to determine the acute toxicity of the feed composition containing the feed additive containing turmeric and stevia mixture, 30 eel were dropped into the aquarium and the feed compositions of Examples 1 to 3 were administered for 10 days for toxicity evaluation. .
구체적으로, 수온을 27±2℃로 유지시킨 수족관(120×40×40 cm)에서 어린 뱀장어(8~12 cm)를 10일 간 양식하였고, 뱀장어는 실험에 사용되기 전 1주일 정도 순화시켰다. 실험에 사용된 뱀장어 개체 수는 수족관당 30마리로 하였으며, 실시예 1 내지 3의 배합비율(0.5 내지 10 중량%의 사료첨가제 조성물)의 사료를 매일 20g씩 두 차례에 걸쳐 투여하였으며, 대조군으로는 울금 및 스테비아 혼합물이 포함되지 않은 일반 사료만을 급이하였다. Specifically, young eel (8-12 cm) was cultured for 10 days in an aquarium (120 × 40 × 40 cm) maintained at a water temperature of 27 ± 2 ° C., and the eel was purified for 1 week before being used in the experiment. The number of eel used in the experiment was 30 per aquarium, and the feed of the compounding ratio (0.5 to 10% by weight of the feed additive composition) of Examples 1 to 3 was administered twice a day 20g twice, as a control. Only normal feeds containing no turmeric and stevia mixtures were fed.
표 3
구분 1일 투여량(g) 생존율
실시예 1 40 30 마리 (100%)
실시예 2 40 30 마리 (100%)
실시예 3 40 30 마리 (100%)
TABLE 3
division Daily Dose (g) Survival rate
Example 1 40 30 (100%)
Example 2 40 30 (100%)
Example 3 40 30 (100%)
그 결과, 울금 및 스테비아 혼합물이 포함된 사료첨가제를 함유한 실시예 1 내지 3의 사료를 투여한 10일 동안 100%의 생존율을 보여 독성이 없는 것을 확인하였다.As a result, it was confirmed that there is no toxicity by showing a survival rate of 100% for 10 days when the feed of Example 1 to 3 containing the feed additive containing turmeric and stevia mixture.
실험예 2: 울금 및 스테비아 혼합물이 포함된 사료첨가제를 함유한 사료 조성물의 뱀장어 생육증진 효과Experimental Example 2: Effect of Eel Growth on Feed Composition Containing Feed Additives Containing Turmeric and Stevia Mixtures
울금 및 스테비아 혼합물이 포함된 사료첨가제를 함유한 사료의 첨가에 의한 뱀장어의 생육증진효과를 알아보기 위해, (i)울금 및 스테비아 혼합물이 포함된 사료첨가제를 함유한, 상기 실시예 3 및 실시예 4의 배합비율(0.5 및 0.3 중량%)을 가진 사료를 78일간 급이하여 양식한 실험구와, (ii)울금 및 스테비아 혼합물이 포함되지 않은 사료첨가제를 함유한 사료를 78일간 급이하여 양식한 뱀장어의 미당 사료섭취량, 미당 증체량 등을 측정하였다.In order to investigate the growth-promoting effect of the eel by the addition of the feed containing the feed additive containing turmeric and stevia mixture, (i) Examples 3 and Example, containing a feed additive containing the turmeric and stevia mixture The experimental group was fed for 78 days with feed having a blending ratio of 0.5 and 0.3 wt%, and the feed containing feed additives containing no turmeric and stevia mixture was fed for 78 days. The amount of feed consumed and the amount of sugar added in the eel was measured.
구체적으로, 뱀장어 양식을 위한 수조는 자동온도(28±1.0℃) 조절, 산소공급, 직사광선 차광, 및 고요한 환경을 유지하기 위하여 차광막이 설치된 온실에 18m×18m×1.7m 크기의 양식수조에 뱀장어 치어를 공시하여 실시하였으며, 대조구는 평균 개시체중 21g의 어류 35,938미를, 실험구는 평균 개시체중 35.7g의 어류를 각각 18,452미, 20,804미를 공시하였다. Specifically, the tank for eel farming is eel in an 18m × 18m × 1.7m sized aquarium in a greenhouse equipped with a shading screen to maintain automatic temperature (28 ± 1.0 ℃) control, oxygen supply, direct sunlight shading, and a quiet environment. The control group showed a total of 35,938 tailings of 21g of fish in the average initiator, and the experimental group of 18,452 tailings and 20,804 tailings of 35.7g of fish in the average initiator, respectively.
먹이통은 각 양식수조 벽면에 부착된 장치에 적당한 크기로 수면 바로 아래 장착하였으며, 급이사료는 사료제조기를 이용하여 반죽형태로 만들어서 급이하였다. 실험식이를 투여한 78일간의 각 양식수조에서의 뱀장어의 사육 결과를 하기 표 4에 나타내었다.The feeder was mounted just below the surface of water in a suitable size to the device attached to the wall of each aquaculture tank, and the feed was fed by making it into a dough form using a feed maker. The results of breeding eel in each cultured tank for 78 days when the experimental diet was administered are shown in Table 4 below.
표 4
Items 대조군 사료첨가제 첨가량(%)
0.3%(실시예 4) 0.5%(실시예 3)
개시어종 총무게(kg) 755 659 743
총 사육미수(미) 35,938 18,452 20,804
미당 개시어 체중(g) 21.0 35.7 35.7
미당 종료어 체중(g)(총 종료 어체중. kg) 71.8(2,580) 127.9(2,360) 128.3(2,670)
미당 사료섭취량(g)(총 종료 사료섭취량, kg) 71.8(2,579.7) 115.1(2,124.2) 109.0(2,267.9)
미당 증체량(g)(총 종료 증체량, kg) 50.8(1,825) 92.2(1,701) 92.6(1,927)
사료효율(%) 70.74 80.08 84.97
사료전환계수 1.41 1.25 1.18
사료급이율(%) 1.81 1.60 1.51
Table 4
Items Control Feed additive amount (%)
0.3% (Example 4) 0.5% (Example 3)
Starting fish species total weight (kg) 755 659 743
Total breeding number (US) 35,938 18,452 20,804
Sugared starting weight (g) 21.0 35.7 35.7
End sugar weight (g) (total end weight. Kg) 71.8 (2,580) 127.9 (2,360) 128.3 (2,670)
Feed intake in micrograms (g) (total feed intake in kg, kg) 71.8 (2,579.7) 115.1 (2,124.2) 109.0 (2,267.9)
Gross weight gain (g) (total weight gain, kg) 50.8 (1,825) 92.2 (1,701) 92.6 (1,927)
Feed efficiency (%) 70.74 80.08 84.97
Feed conversion factor 1.41 1.25 1.18
Feed rate (%) 1.81 1.60 1.51
그 결과, (i) 뱀장어의 미당 증체량은 대조구(50.8g)에 비해 울금 및 스테비아 혼합물이 포함된 사료첨가제를 첨가한 실험구(92.2g 및 92.6g)에서 2배 가까이 증가하였으며, (ii) 증체량의 기준으로서 미당 사료섭취량 또한 대조구(71.8g)에 비하여 실험구(115.1g 및 109.0g)에서 현저히 증가하였음을 확인하였다. 따라서, 울금 및 스테비아 혼합물이 포함된 사료첨가제를 첨가한 실험구의 증가된 사료 섭취량이 개체의 증체량에 영향을 미칠 수 있음을 시사한다.As a result, (i) the weight gain of the eel increased almost twice in the experimental group (92.2g and 92.6g) with feed additives containing turmeric and stevia compared to the control (50.8g), and (ii) As a criterion, feed intake was also significantly increased in the experimental group (115.1g and 109.0g) compared to the control (71.8g). Thus, the increased feed intake of the experimental group to which the feed additive containing turmeric and stevia mixture may be added may affect the weight gain of the individual.
(iii) 사료효율을 계산한 결과, 대조구(70.74%)에 비하여 울금 및 스테비아 혼합물이 포함된 사료첨가제를 첨가한 실험구(80.08% 및 84.97%)가 더 높은 사료효율을 보였으며, (iv) 사료전환계수를 계산한 결과, 대조구(1.41)에 비하여 실험구(1.25 및 1.18)에서 더 낮은 값을 보임으로써 울금 및 스테비아 혼합물이 포함된 사료첨가제의 상당한 생육증진 효과를 확인할 수 있었다.(iii) As a result of calculating feed efficiency, the experimental group (80.08% and 84.97%) with feed additive containing turmeric and stevia mixture showed higher feed efficiency than the control (70.74%), and (iv) As a result of calculating the feed conversion coefficient, the lower value was shown in the experimental group (1.25 and 1.18) than the control group (1.41), and the significant growth promoting effect of the feed additive containing turmeric and stevia mixture was confirmed.
한편, 울금 및 스테비아 혼합물이 포함된 사료첨가제를 함유한 사료의 첨가에 의한 뱀장어의 사료기호도 증진 여부를 알아보기 위해, (i) 울금 및 스테비아 혼합물이 포함된 사료첨가제를 함유한, 상기 실시예 3 및 실시예 4의 배합비율(0.5 및 0.3 중량%)을 가진 사료를 급이하여 양식한 실험구와, (ii) 울금 및 스테비아 혼합물이 포함되지 않은 사료첨가제를 함유한 사료를 급이하여 양식한 뱀장어의 사료섭취 시간을 측정하였고, 그 결과를 하기 표 5에 나타내었다.On the other hand, in order to find out whether the addition of the feed containing the feed additive containing turmeric and stevia mixture to enhance the feed symbol of the eel, (i) containing the feed additive containing turmeric and stevia mixture, Example 3 And an eel cultured by feeding a feed containing a blending ratio (0.5 and 0.3% by weight) of Example 4, and (ii) a feed containing a feed additive not containing turmeric and stevia mixture. Feed intake time was measured, and the results are shown in Table 5 below.
표 5
항목 대조구 사료첨가제 첨가비율 (중량%)
0.3 0.5
오전 오후 오전 오후 오전 오후
최대(분) 8.40 9.30 5.40 4.50 6.00 4.50
최소(분) 2.20 2.30 2.00 1.30 1.50 1.55
평균(분) 3.76 3.76 2.63 2.45 2.60 2.62
Table 5
Item Control Feed Additive Ratio (wt%)
0.3 0.5
morning afternoon morning afternoon morning afternoon
Max (minutes) 8.40 9.30 5.40 4.50 6.00 4.50
Min (minutes) 2.20 2.30 2.00 1.30 1.50 1.55
Average minutes 3.76 3.76 2.63 2.45 2.60 2.62
그 결과, 대조구의 평균 섭취시간(3.76분) 보다 실험구의 평균 섭취시간(2.45 내지 2.63분)이 더 짧아 대조구보다 실험군에서 사료를 더 빨리 섭취하였음을 확인하였다. 이는 울금 및 스테비아 혼합물이 포함된 사료첨가제의 첨가로 인한 뱀장어의 사료 기호도가 증가하였음을 시사한다.As a result, the average intake time (2.45 to 2.63 minutes) of the experimental group was shorter than the average intake time (3.76 minutes) of the control group was confirmed that the feed was ingested faster in the experimental group than the control group. This suggests that the food acceptability of eel increased due to the addition of feed additives containing turmeric and stevia mixtures.
추가적으로, (i) 울금 및 스테비아 혼합물이 포함된 사료첨가제를 함유한, 상기 실시예 3의 배합비율(0.5 중량%)을 가진 사료를 30주간 급이하여 사육한 실험구와, (ii) 울금 및 스테비아 혼합물이 포함되지 않은 사료첨가제를 함유한 사료를 30주간 급이하여 사육한 뱀장어의 평균 무게를 측정함으로써 발육상태를 비교하였고 그 결과는 하기 표 6에 나타내었다.In addition, (i) the experimental group in which the feed having the blending ratio (0.5% by weight) of Example 3 containing feed additives containing turmeric and stevia mixture was fed for 30 weeks, and (ii) turmeric and stevia The growth state was compared by measuring the average weight of the eel reared by feeding the feed additive containing no mixture for 30 weeks and the results are shown in Table 6 below.
표 6
사육기간 뱀장어 평균 무게(g)
대조구 실험구(실시예 3)
30주 230±12 400±15
Table 6
Breeding period Eel average weight (g)
Control Experimental Zone (Example 3)
30 Weeks 230 ± 12 400 ± 15
그 결과, 대조구의 평균 무게(230g) 보다 실험구의 평균 무게(400g)가 현저히 높았으며, 1kg 당 마리수는 실험구가 2.5마리, 대조구가 4.5마리였다. 이는 울금 및 스테비아 혼합물이 포함된 사료첨가제의 우수한 생육 증진효과를 뒷받침한다.As a result, the average weight (400g) of the experimental group was significantly higher than the average weight (230g) of the control, the number of animals per 1kg was 2.5 animals and 4.5 control animals. This supports the good growth promoting effect of feed additives containing turmeric and stevia mixture.
실험예 3: 울금 및 스테비아 혼합물이 포함된 사료첨가제를 함유한 사료 조성물을 급이한 양식 뱀장어의 성분 변화Experimental Example 3: Changes in Components of Cultured Eels Feeding Feed Composition Containing Feed Additives Containing Turmeric and Stevia Mixtures
울금 및 스테비아 혼합물이 포함된 사료첨가제를 함유한 사료의 첨가에 의한 뱀장어의 성분변화를 알아보기 위해, (i) 울금 및 스테비아 혼합물이 포함된 사료첨가제를 함유한, 상기 실시예 3의 배합비율(0.5 중량%)을 가진 사료를 30주간 급이하여 양식한 실험구와, (ii) 울금 및 스테비아 혼합물이 포함되지 않은 사료첨가제를 함유한 사료를 30주간 급이하여 양식한 뱀장어의 수분, 조회분, 조지방, 조단백질, 비타민 C 및 비타민 E의 함량을 평가하였다. 그 결과는 하기 표 7에 나타내었다.In order to examine the changes in the eel composition by the addition of the feed containing the feed additive containing turmeric and stevia mixture, (i) the blending ratio of Example 3 containing the feed additive containing turmeric and stevia mixture ( 0.5 wt%) of the eel cultured by feeding the feed containing 30% of the feed, and (ii) the water, crude ash, Crude fat, crude protein, vitamin C and vitamin E contents were evaluated. The results are shown in Table 7 below.
표 7
수분(%) 조회분(%) 조지방(%) 조단백질(%) 비타민C (mg/Kg) 비타민E (mg/Kg)
대조구 64.30±0.27 1.96±0.01 17.43±0.06 16.83±0.04 0.83±0.00 2.42±0.05
실험구 61.58±0.30 2.06±0.02 19.34±0.05 17.61±0.01 74.49±0.23 2.32±0.06
TABLE 7
moisture(%) % Viewed Crude fat (%) Crude Protein (%) Vitamin C (mg / Kg) Vitamin E (mg / Kg)
Control 64.30 ± 0.27 1.96 ± 0.01 17.43 ± 0.06 16.83 ± 0.04 0.83 ± 0.00 2.42 ± 0.05
Experiment 61.58 ± 0.30 2.06 ± 0.02 19.34 ± 0.05 17.61 ± 0.01 74.49 ± 0.23 2.32 ± 0.06
그 결과, (i) 수분함량은 실험구에 비하여 대조구에서 다소 높게 측정된 반면, (ii) 조회분, 조지방, 조단백질의 함량은 대조구에 비하여 실험구에서 다소 높게 측정되었다. (iii) 특히, 비타민 C의 함량은 대조구(0.83 mg/Kg)보다 실험구(74.49 mg/Kg)에서 현저히 증가하였음을 확인하였는 바, 본 발명의 울금 및 스테비아 혼합물은 생육증진뿐만 아니라 고기능 뱀장어를 양식할 수 있는 사료를 제공할 수 있음을 시사한다.As a result, (i) the moisture content was slightly higher in the control than the control, and (ii) the crude ash, crude fat, and crude protein were slightly higher in the control than the control. (iii) In particular, it was confirmed that the content of vitamin C was significantly increased in the experimental group (74.49 mg / Kg) than the control (0.83 mg / Kg), the turmeric and stevia mixture of the present invention not only promotes growth but also high-performance eel Suggests that feed can be farmed.
실험예 4: 울금 및 스테비아 혼합물이 포함된 사료첨가제를 함유한 사료 조성물을 급이한 양식장 수질 개선효과Experimental Example 4: Water quality improvement effect of feed composition containing feed additive containing turmeric and stevia mixture
울금 및 스테비아 혼합물이 포함된 사료첨가제를 함유한 사료의 첨가에 의한 양식장 수질 개선효과를 알아보기 위해, (i) 울금 및 스테비아 혼합물이 포함된 사료첨가제를 함유한, 상기 실시예 3 및 실시예 4의 배합비율(0.5 및 0.3 중량%)을 가진 사료를 급이하여 양식한 양식수(실험구)와, (ii) 울금 및 스테비아 혼합물이 포함되지 않은 사료첨가제를 함유한 사료를 급이하여 양식한 양식수(대조구)의 pH, 용존산소량, 질산성 질소, 암모니아성 질소의 변화를 비교하였고, 그 결과를 하기 표 8에 나타내었다.In order to examine the effect of improving the water quality of the farm by adding feed containing feed additive containing turmeric and stevia mixture, (i) Examples 3 and 4 containing feed additive containing turmeric and stevia mixture Aquaculture water (experimental) fed with feed having a blending ratio of 0.5 and 0.3 wt%, and (ii) feed containing feed additives containing no turmeric and stevia mixture The pH, dissolved oxygen, nitrate nitrogen, and ammonia nitrogen of the cultured water (control) were compared, and the results are shown in Table 8 below.
표 8
항목 대조구 사료첨가제 첨가율 (%)
0.3(실시예 4) 0.5(실시예 3)
pH 최소 4.60 4.77 4.75
최대 6.30 7.14 7.15
평균 5.39±0.45 5.69±0.60 5.97±0.46
변이계수(%) 8.28 10.56 7.76
DO(ppm) 최소 6.3 6.0 6.1
최대 7.5 7.7 7.2
평균 6.8±0.31 6.6±0.50 6.45±0.30
변이계수(%) 4.55 7.6 4.71
NO2-N(ppm) 최소 1 1 1
최대 10 10 10
평균 4.49±0.88 4.0±0.28 3.8±0.54
변이계수(%) 19.7 28.4 54.2
NH3-N(ppm) 최소 3 1 1
최대 12 10 4
평균 9.9±1.74 5.38±2.30 2.52±1.13
변이계수(%) 17.6 42.7 44.9
Table 8
Item Control Feed additive addition rate (%)
0.3 (Example 4) 0.5 (Example 3)
pH at least 4.60 4.77 4.75
maximum 6.30 7.14 7.15
Average 5.39 ± 0.45 5.69 ± 0.60 5.97 ± 0.46
Coefficient of variation (%) 8.28 10.56 7.76
DO (ppm) at least 6.3 6.0 6.1
maximum 7.5 7.7 7.2
Average 6.8 ± 0.31 6.6 ± 0.50 6.45 ± 0.30
Coefficient of variation (%) 4.55 7.6 4.71
NO 2 -N (ppm) at least One One One
maximum 10 10 10
Average 4.49 ± 0.88 4.0 ± 0.28 3.8 ± 0.54
Coefficient of variation (%) 19.7 28.4 54.2
NH 3 -N (ppm) at least 3 One One
maximum 12 10 4
Average 9.9 ± 1.74 5.38 ± 2.30 2.52 ± 1.13
Coefficient of variation (%) 17.6 42.7 44.9
그 결과, 8.28%의 변이계수가 도출된 대조구와 달리, 울금 및 스테비아 혼합물이 포함된 사료첨가제를 함유한 사료를 급이한 실험구에서는 7.76%의 더 낮은 값이 도출되었다. 이는 울금 및 스테비아 혼합물이 포함된 사료첨가제를 양식에 사용함으로써 양식조의 수질환경의 변화가 거의 없는 안정적 수질관리를 할 수 있음은 물론, 경제적으로도 물의 환수 횟수를 줄여주는 효과를 나타낸다 할 것이다.As a result, a lower value of 7.76% was obtained in the experimental diet fed the feed containing the feed additive containing turmeric and stevia mixture, unlike the control with 8.28% variation coefficient. The use of feed additives containing turmeric and stevia mixtures in aquaculture can provide stable water quality management with little change in the water environment of the aquaculture tanks, as well as economically reducing the number of water retrieval.
또한, (i) 실시예 3의 배합비율(0.5 중량%)을 가진 사료를 급이하여 양식한 양식수(실험구)와, (ii) 울금 및 스테비아 혼합물이 포함되지 않은 사료첨가제를 함유한 사료를 급이하여 양식한 양식수(대조구)의 부유물질의 양, 총 질소량 및 화학적 산소요구량을 측정하여 수질오염의 정도를 확인하고자 하였고, 그 결과를 하기 표 9에 나타내었다.In addition, (i) aquaculture water (experimental) cultured by feeding a feed having a blending ratio (0.5% by weight) of Example 3, and (ii) a feed containing a feed additive not containing turmeric and stevia mixture. In order to determine the degree of water pollution by measuring the amount of suspended solids, total nitrogen and chemical oxygen demand of aquaculture water (control), which were farmed by feeding water, the results are shown in Table 9 below.
표 9
검사항목 대조구 실험구(실험예 3)
화학적 산소요구량(mg/L) 42.3 25.0
부유물질(mg/L) 95.0 16.0
총 질소(mg/L) 116.6 104.6
Table 9
Inspection items Control Experiment Zone (Experimental Example 3)
Chemical oxygen demand (mg / L) 42.3 25.0
Suspended solids (mg / L) 95.0 16.0
Total Nitrogen (mg / L) 116.6 104.6
그 결과, (i) 부유물질의 경우, 대조구(95 mg/L)에 비하여 실험구(16.0 mg/L)에서 6배 이상 감소한 수치가 측정되었고, (ii) 화학적 산소요구량도 실험구가(20 mg/L) 대조구(42.3 mg/L)보다 약 2배 이상 낮은 값을 가졌다. 또한 (iii) 총 질소량도 대조구(116.6 mg/L)보다 실험구(104.6 mg/L)에서 더 낮게 측정되었다. 이는 본 발명의 울금 및 스테비아 혼합물은 양식수의 수질환경의 개선효과가 탁월함을 시사하는 것이다.As a result, (i) in the case of suspended solids, the value decreased more than 6 times in the experimental group (16.0 mg / L) compared to the control group (95 mg / L), and (ii) the chemical oxygen demand was also reduced (20). mg / L) had a value about 2 times lower than the control (42.3 mg / L). In addition, (iii) the total nitrogen was also lower in the experimental group (104.6 mg / L) than the control (116.6 mg / L). This suggests that the turmeric and stevia mixture of the present invention is excellent in improving the water environment of the cultured water.

Claims (11)

  1. 울금 및, 스테비아 또는 스테비오사이드의 혼합물을 유효성분으로 포함하는 뱀장어 생육증진용 사료첨가제 조성물.Feed additive composition for eel growth promotion comprising turmeric and a mixture of stevia or stevioside as an active ingredient.
  2. 제1항에 있어서, 상기 사료첨가제는 생육증진 및 양식수 수질 정화기능을 갖는 것인, 사료첨가제 조성물.The feed additive composition of claim 1, wherein the feed additive has growth promoting and aquaculture water purification functions.
  3. 제1항에 있어서, 울금 및, 스테비아 또는 스테비오사이드의 혼합물을 전체 조성물 중 0.1 내지 0.5 중량%의 비율로 포함하는 것인, 사료첨가제 조성물.The feed additive composition of claim 1, comprising turmeric and a mixture of stevia or stevioside in a proportion of 0.1 to 0.5% by weight of the total composition.
  4. 제1항에 있어서, 상기 울금 및 스테비아 혼합물은 분말 형태인 것인, 사료첨가제 조성물.The feed additive composition of claim 1, wherein the turmeric and stevia mixture is in powder form.
  5. 제1항에 있어서, 상기 울금 및 스테비아는 1 : 1 내지 1 : 3의 혼합비로 존재하는 것인, 사료첨가제 조성물.The feed additive composition of claim 1, wherein the turmeric and stevia are present in a mixing ratio of 1: 1 to 1: 3.
  6. 제1항 내지 제5항 중 어느 한 항의 사료첨가제 조성물을 포함하는 사료.A feed comprising the feed additive composition of any one of claims 1 to 5.
  7. 울금 및, 스테비아 또는 스테비오사이드의 혼합물을 유효성분으로 포함하는 뱀장어 생육증진용 및 양식수 수질 정화용 사료첨가제 조성물.Feed additive composition for improving eel growth and purifying water quality of the eel, including turmeric and a mixture of stevia or stevioside as an active ingredient.
  8. 울금 및, 스테비아 또는 스테비오사이드의 혼합물을 뱀장어에 급이하는 단계를 포함하는 뱀장어 생육을 증진시키는 방법.Feeding turmeric and a mixture of stevia or stevioside to the eel.
  9. 제8항에 있어서, 상기 울금 및 스테비아는 1 : 1 내지 1 : 3의 혼합비로 존재하는 것인, 방법.The method of claim 8, wherein the turmeric and stevia are present in a mixing ratio of 1: 1 to 1: 3.
  10. 울금 및, 스테비아 또는 스테비오사이드의 혼합물을 뱀장어 양식수에 투여하는 단계를 포함하는 양식수의 수질을 개선시키는 방법.A method of improving the water quality of cultured water comprising administering turmeric and a mixture of stevia or stevioside to eel cultured water.
  11. 제10항에 있어서, 상기 울금 및 스테비아는 1 : 1 내지 1 : 3의 혼합비로 존재하는 것인, 방법.The method of claim 10, wherein the turmeric and stevia are present in a mixing ratio of 1: 1 to 1: 3.
PCT/KR2015/004450 2014-05-02 2015-05-01 Feed additive composition containing mixture of turmeric and stevia as active ingredient for promoting growth of anguilla japonica WO2015167310A1 (en)

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KR102084759B1 (en) * 2018-09-14 2020-03-04 (주)셀트리랩 Feedstuffs for fish cultivation and methods for their production
KR102228035B1 (en) * 2020-11-03 2021-03-15 농업회사법인 에버그린팜 주식회사 Method of producing eco-friendly quail feed composition containing bamboo salt

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