WO2015191911A3 - Microvésicules enrichies en protéines et leurs procédés de fabrication et d'utilisation - Google Patents
Microvésicules enrichies en protéines et leurs procédés de fabrication et d'utilisation Download PDFInfo
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- WO2015191911A3 WO2015191911A3 PCT/US2015/035413 US2015035413W WO2015191911A3 WO 2015191911 A3 WO2015191911 A3 WO 2015191911A3 US 2015035413 W US2015035413 W US 2015035413W WO 2015191911 A3 WO2015191911 A3 WO 2015191911A3
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
- C12N15/88—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation using microencapsulation, e.g. using amphiphile liposome vesicle
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- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction
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- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/85—Fusion polypeptide containing an RNA binding domain
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/111—General methods applicable to biologically active non-coding nucleic acids
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N2310/00—Structure or type of the nucleic acid
- C12N2310/10—Type of nucleic acid
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2310/00—Structure or type of the nucleic acid
- C12N2310/10—Type of nucleic acid
- C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPRs]
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2320/00—Applications; Uses
- C12N2320/30—Special therapeutic applications
- C12N2320/32—Special delivery means, e.g. tissue-specific
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2795/00—Bacteriophages
- C12N2795/00011—Details
- C12N2795/18011—Details ssRNA Bacteriophages positive-sense
- C12N2795/18111—Leviviridae
- C12N2795/18122—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2810/00—Vectors comprising a targeting moiety
- C12N2810/50—Vectors comprising as targeting moiety peptide derived from defined protein
- C12N2810/60—Vectors comprising as targeting moiety peptide derived from defined protein from viruses
- C12N2810/6072—Vectors comprising as targeting moiety peptide derived from defined protein from viruses negative strand RNA viruses
- C12N2810/6081—Vectors comprising as targeting moiety peptide derived from defined protein from viruses negative strand RNA viruses rhabdoviridae, e.g. VSV
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- Genetics & Genomics (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Wood Science & Technology (AREA)
- Organic Chemistry (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Plant Pathology (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Peptides Or Proteins (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
La présente invention concerne une microvésicule comprenant les éléments suivants : (i) une protéine associée à une membrane comportant au moins un premier domaine de dimérisation ; (ii) une protéine porteuse comprenant au moins un second domaine de dimérisation ; et (iii) un soluté qui se lie à la protéine porteuse, ledit soluté étant choisi dans le groupe constitué de l'ADN, l'ARN, les protéines, les glucides, les ribosomes, les mitochondries, et les petites molécules. La présente invention concerne en outre des cellules, des réactifs et des kits utiles dans la fabrication des microvésicules, ainsi que des procédés d'utilisation desdites microvésicules, par exemple, dans la recherche et pour des applications thérapeutiques.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US201462011528P | 2014-06-12 | 2014-06-12 | |
US62/011,528 | 2014-06-12 |
Publications (2)
Publication Number | Publication Date |
---|---|
WO2015191911A2 WO2015191911A2 (fr) | 2015-12-17 |
WO2015191911A3 true WO2015191911A3 (fr) | 2016-03-31 |
Family
ID=54834565
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/US2015/035413 WO2015191911A2 (fr) | 2014-06-12 | 2015-06-11 | Microvésicules enrichies en protéines et leurs procédés de fabrication et d'utilisation |
Country Status (1)
Country | Link |
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WO (1) | WO2015191911A2 (fr) |
Families Citing this family (26)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP2734621B1 (fr) | 2011-07-22 | 2019-09-04 | President and Fellows of Harvard College | Évaluation et amélioration de la spécificité de clivage des nucléases |
US9163284B2 (en) | 2013-08-09 | 2015-10-20 | President And Fellows Of Harvard College | Methods for identifying a target site of a Cas9 nuclease |
US9359599B2 (en) | 2013-08-22 | 2016-06-07 | President And Fellows Of Harvard College | Engineered transcription activator-like effector (TALE) domains and uses thereof |
US9737604B2 (en) | 2013-09-06 | 2017-08-22 | President And Fellows Of Harvard College | Use of cationic lipids to deliver CAS9 |
US9340800B2 (en) | 2013-09-06 | 2016-05-17 | President And Fellows Of Harvard College | Extended DNA-sensing GRNAS |
US9322037B2 (en) | 2013-09-06 | 2016-04-26 | President And Fellows Of Harvard College | Cas9-FokI fusion proteins and uses thereof |
US20150165054A1 (en) | 2013-12-12 | 2015-06-18 | President And Fellows Of Harvard College | Methods for correcting caspase-9 point mutations |
US10077453B2 (en) | 2014-07-30 | 2018-09-18 | President And Fellows Of Harvard College | CAS9 proteins including ligand-dependent inteins |
JP7067793B2 (ja) | 2015-10-23 | 2022-05-16 | プレジデント アンド フェローズ オブ ハーバード カレッジ | 核酸塩基編集因子およびその使用 |
JP7231935B2 (ja) | 2016-08-03 | 2023-03-08 | プレジデント アンド フェローズ オブ ハーバード カレッジ | アデノシン核酸塩基編集因子およびそれらの使用 |
AU2017308889B2 (en) | 2016-08-09 | 2023-11-09 | President And Fellows Of Harvard College | Programmable Cas9-recombinase fusion proteins and uses thereof |
US11542509B2 (en) | 2016-08-24 | 2023-01-03 | President And Fellows Of Harvard College | Incorporation of unnatural amino acids into proteins using base editing |
KR20240007715A (ko) | 2016-10-14 | 2024-01-16 | 프레지던트 앤드 펠로우즈 오브 하바드 칼리지 | 핵염기 에디터의 aav 전달 |
WO2018119359A1 (fr) | 2016-12-23 | 2018-06-28 | President And Fellows Of Harvard College | Édition du gène récepteur ccr5 pour protéger contre l'infection par le vih |
TW201839136A (zh) | 2017-02-06 | 2018-11-01 | 瑞士商諾華公司 | 治療血色素異常症之組合物及方法 |
US11866699B2 (en) | 2017-02-10 | 2024-01-09 | University Of Washington | Genome editing reagents and their use |
US11898179B2 (en) | 2017-03-09 | 2024-02-13 | President And Fellows Of Harvard College | Suppression of pain by gene editing |
EP3592777A1 (fr) | 2017-03-10 | 2020-01-15 | President and Fellows of Harvard College | Éditeur de base cytosine à guanine |
KR20190130613A (ko) | 2017-03-23 | 2019-11-22 | 프레지던트 앤드 펠로우즈 오브 하바드 칼리지 | 핵산 프로그램가능한 dna 결합 단백질을 포함하는 핵염기 편집제 |
WO2018209320A1 (fr) | 2017-05-12 | 2018-11-15 | President And Fellows Of Harvard College | Arn guides incorporés par aptazyme pour une utilisation avec crispr-cas9 dans l'édition du génome et l'activation transcriptionnelle |
JP2020534795A (ja) | 2017-07-28 | 2020-12-03 | プレジデント アンド フェローズ オブ ハーバード カレッジ | ファージによって支援される連続的進化(pace)を用いて塩基編集因子を進化させるための方法および組成物 |
WO2019139645A2 (fr) | 2017-08-30 | 2019-07-18 | President And Fellows Of Harvard College | Éditeurs de bases à haut rendement comprenant une gam |
WO2019079347A1 (fr) | 2017-10-16 | 2019-04-25 | The Broad Institute, Inc. | Utilisations d'éditeurs de bases adénosine |
JP2021524272A (ja) * | 2018-07-10 | 2021-09-13 | エーエルアイエー セラピューティクス エス. アール. エル. | ガイドrna分子および/またはガイドrna分子/rna誘導型ヌクレアーゼ複合体の無痕跡送達のための小胞およびその産生方法 |
EP3942040A1 (fr) | 2019-03-19 | 2022-01-26 | The Broad Institute, Inc. | Procédés et compositions pour l'édition de séquences nucléotidiques |
WO2021226558A1 (fr) | 2020-05-08 | 2021-11-11 | The Broad Institute, Inc. | Méthodes et compositions d'édition simultanée des deux brins d'une séquence nucléotidique double brin cible |
Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2011058064A1 (fr) * | 2009-11-13 | 2011-05-19 | INSERM (Institut National de la Santé et de la Recherche Médicale) | Reprogrammation de cellules eucaryotes par des microvésicules obtenues par génie génétique |
US20130323220A1 (en) * | 2012-05-29 | 2013-12-05 | The General Hospital Corporation | TAL-Tet1 Fusion Proteins and Methods of Use Thereof |
WO2013188522A2 (fr) * | 2012-06-12 | 2013-12-19 | Genentech, Inc. | Procédés et compositions pour la génération d'allèles à inactivation conditionnelle |
WO2014036391A1 (fr) * | 2012-08-30 | 2014-03-06 | Exosome Diagnostics, Inc. | Témoins pour dosages d'acide nucléique |
-
2015
- 2015-06-11 WO PCT/US2015/035413 patent/WO2015191911A2/fr active Application Filing
Patent Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2011058064A1 (fr) * | 2009-11-13 | 2011-05-19 | INSERM (Institut National de la Santé et de la Recherche Médicale) | Reprogrammation de cellules eucaryotes par des microvésicules obtenues par génie génétique |
US20130323220A1 (en) * | 2012-05-29 | 2013-12-05 | The General Hospital Corporation | TAL-Tet1 Fusion Proteins and Methods of Use Thereof |
WO2013188522A2 (fr) * | 2012-06-12 | 2013-12-19 | Genentech, Inc. | Procédés et compositions pour la génération d'allèles à inactivation conditionnelle |
WO2014036391A1 (fr) * | 2012-08-30 | 2014-03-06 | Exosome Diagnostics, Inc. | Témoins pour dosages d'acide nucléique |
Non-Patent Citations (1)
Title |
---|
LIMIN LI ET AL.: "Argonaute 2 Complexes Selectively Protect the Circulating MicroRNAs in Cell -Secreted Microvesicles", PLOS ONE, vol. 7, no. 10, 2012, pages 1 - 9 * |
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Publication number | Publication date |
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WO2015191911A2 (fr) | 2015-12-17 |
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