WO2015174772A9

WO2015174772A9 - Skin lifting cosmetic composition containing chitosan having fast dissolving property

Info

Publication number: WO2015174772A9
Application number: PCT/KR2015/004872
Authority: WO
Inventors: 손미경; 박순영
Original assignee: 손미경; 박순영
Priority date: 2014-05-14
Filing date: 2015-05-14
Publication date: 2016-06-09
Also published as: KR101850053B1; KR20140113614A; KR20150132011A; KR101596634B1; KR20150132039A; WO2015174772A1

Abstract

The present invention relates to a cosmetic composition which: does not contain a preservative, a surfactant and the like capable of burdening or having an adverse effect on the skin since the cosmetic composition contains chitosan having a fast dissolving property in an undissolved state and is used by allowing chitosan to be dissolved by a user just before use; and is suitable for skin lifting, the cosmetic composition comprising: 0.5-6.0 wt% of chitosan, having a fast dissolving property, dissolved with a dissolution velocity, in the range of 1-15 minutes, in a solvent having a degree of deacetylation (DA) in the range of 80-100% and a pH in the range of 3.5-7.5; and the remainder being a solvent having a pH in the range of 3.5-7.5, wherein the chitosan is physically separated from the solvent and contained in an undissolved state.

Description

Cosmetic composition for skin lifting containing chitosan having fast solubility

The present invention relates to a cosmetic composition for lifting a skin comprising chitosan having a quick solubility, and in particular, comprises chitosan having a quick solubility in a state of beauty and dissolves by the user immediately before use, thereby burdening or adversely affecting the skin. It does not contain preservatives and surfactants that can give, and relates to a cosmetic composition suitable for skin lifting.

The problem of environmental pollution is getting serious and the cause of skin trouble is increasing, and more and more people are looking for cosmetics made from natural ingredients. In addition, as interest in well-being increases, natural raw materials are naturally taking place as the beginning of the well-being lifestyle method based on raw material characteristics. Natural is the central concept of eternal cosmetics, and how to improve and advance it is a challenge.

The cosmetics market shows a three-dimensional market structure in which the vertical axis of 'health' is added to the two-dimensional structure of 'natural' and 'function'. Each element of natural, function, and health is strengthened through the convergence or simplicity of each other in terms of beauty. This 'healthy beauty' should begin by taking away the bad rather than adding the good. No matter how many good ingredients are present, if various chemicals coexist for their fusion, they will never produce good results for the skin.

Unless cosmetics are a pharmaceutical, it is important to use safer ingredients, fresher products, and more functional products, which are safer for the skin than immediate effects, and these consumer desires are already becoming a global trend. . The shelf life of one to two years, the risk of chemical preservatives, which are essentially contained as a problem of reuse according to the dose, is an inevitable problem for cosmetic users, and many consumers suffer skin problems. In order to escape the danger of such chemical preservatives, the number of consumers making their own natural cosmetics by themselves is increasing every year.

Accordingly, there is a need for the development of instant cosmetics that produce cosmetics on the fly using the safest materials for skin and the properties of the raw materials, and apply the efficacy effect of the raw materials to the skin freshly.

Currently, cosmetics in the market are released as a finished product having different functions and performances by mass production method which is collectively performed in a factory. This is because only a manufacturer who has a certain manufacturing facility and quality control facility under the domestic cosmetics law and is authorized by the authorities can manufacture cosmetics.

Such a factory mass production method is easy to monitor each process so that consumers can safely consume, strict quality control is made, there is an advantage in terms of manufacturer tracking or after-care for side effects or accidents caused by cosmetic consumption.

However, these factory mass production methods have difficulty in activating the personalized cosmetics market because they cannot satisfy various needs of consumers because of the characteristics of standardization, standardization, and mass production, and also cannot actively respond to various needs of consumers.

In addition, cosmetics produced unilaterally according to the needs of companies are supplied regardless of consumer needs, consumption purposes, or tastes.

For example, since a second material included in one cosmetic product is the same but adds a simple efficacy or functional material and then is displayed on the market as another product, a consumer may have a cosmetic product having the same second material but having a different function. New purchases are often necessary.

Accordingly, more and more consumers manufacture cosmetics using natural ingredients at home.

On the other hand, chitosan is a natural animal marine polymer having a unique cation in nature, which is compatible with the human body, has no rejection, no side effects, is non-irritating, and is a safe material for the living body. It has many beneficial effects such as activity, skin protective film formation, and impurity adsorption, but until now it has been mainly recognized and used only as a health food to be ingested, and development as a beneficial cosmetic for skin beauty is insufficient.

The present invention has been made to solve the problems of the prior art as described above, and includes chitosan having a quick-release in a state of beauty, which can be burdened or adversely affected by the skin to be dissolved and used by the user immediately before use. It does not contain preservatives, surfactants, etc., to provide a cosmetic composition suitable for skin lifting.

The cosmetic composition for skin lifting comprising chitosan having a fast dissolving property according to the present invention is a solvent having a degree of deacetylation (DA) within a range of 80 to 100% and a pH within a range of 3.5 to 7.5. 0.5 to 6.0 wt% of chitosan having a fast solubility dissolved at a dissolution rate within a range of 1 to 15 minutes; And a solvent having a pH within a range of 3.5 to 7.5 as a residual amount, wherein the chitosan is physically separated from the solvent and is not dissolved therein.

The chitosan may preferably have a degree of deacetylation within the range of 90 to 100%, more preferably within the range of 95 to 100%.

The chitosan may be fibrous chitosan having an average thickness within the range of 0.010 to 0.050 mm, fibrous chitosan may be included in an amount within the range of 1.7 to 4.0% by weight.

The chitosan may be powdery chitosan having an average thickness within the range of 0.150 to 0.450 mm, powdery chitosan may be included in an amount within the range of 2.6 to 6.0% by weight.

The solvent is water; Or glycolic acid, lactic acid, lactic acid, citric acid, malic acid, tartaric acid, citric acid, ascorbic acid (vitamin C), ascorbyl phosphate, ascorbyl glucoside, magnesium ascorbyl phosphate, sodium ascorbyl phosphate, ascorbic Acid may be dissolved in a pH adjuster selected from the group consisting of bilpalmitate and a mixture of two or more thereof.

According to the present invention, it contains chitosan having a fast solubility in a state of beauty, and does not include preservatives and surfactants that can burden or adversely affect the skin by dissolving it by the user immediately before use, and lifting the skin. By providing a suitable cosmetic composition for the use, it is possible to prevent the danger of chemical preservatives, especially skin troubles, which are pointed out as problems in the prior art, and to use cosmetics immediately when using natural materials that are safe for the skin by using the properties of the raw materials. It is prepared to provide the effect of allowing the skin to apply the efficacy effect of the raw material to the freshest.

1 to 6 are photographs of the fibrous chitosan used in the solubility test according to the thickness of chitosan, in particular fibrous chitosan and powdery chitosan, used in the present invention.

7 is a photograph showing the results of solubility experiment with increasing deacetylation degree.

8 is a photograph showing the state after 30 days elapsed by storing the chitosan solution refrigerated.

9 is a photograph showing a state in which the fungus bacteria cultivated after 10 days by storing the chitosan solution at room temperature.

10 to 16 are graphs showing measurement data values of the eye tail lifting angle before use and after 2 weeks of use, in order to confirm the lifting value change of each wrinkle area.

In the best form according to the present invention, the cosmetic composition for skin lifting comprising chitosan having rapid solubility has a degree of deacetylation (DA) within the range of 80 to 100% and within the range of 3.5 to 7.5. 0.5 to 6.0% by weight of chitosan having a fast solubility dissolved in a solvent having a pH at a dissolution rate within a range of 1 to 15 minutes; And a solvent having a pH within a range of 3.5 to 7.5 as a residual amount, wherein the chitosan is physically separated from the solvent and is not dissolved therein.

Hereinafter, the present invention will be described in detail with reference to specific examples.

In the present invention, the term chitosan is defined as meaning chitosan having water and / or pH within an acidic range and dissolved in acidic water obtained by including an acidic substance in water based on water.

Chitosan, which is a raw material of chitosan, is a deacetylated product obtained by deacetylating chitin obtained from shells of crustaceans, and is soluble in water, in particular weak acids, and is easily absorbed by the human body. It is one of and functions as one ingredient that gives skin lifting effect when applied to the skin. Chitosan is a glucosamine polymer obtained by alkali treatment of chitin, which is found in shellfish such as shrimp, crabs, insect shells, and squid bones. The main component is polyglucosamine, a colorless amorphous powder with a polymer structure. Chitosan, which is a glucosamine bond, has a molecular structure very similar to that of our human tissues, and is very valuable as a food and drug because it has a very good human affinity and no immune response occurs. The degree of deacetylation of chitosan can be measured using colloid titration as described in K. Toei and T. Kohara, Analytica Chimica Acta, 83, 59-65 (1976).

The chitosan may have a degree of deacetylation within the range of 80 to 100%, preferably within the range of 90 to 100%, more preferably within the range of 95 to 100%. When the deacetylation degree is less than 80%, not only the solubility of chitosan decreases but also the amount of undissolved solids increases, which is not suitable for application or application to the skin, such as heavy metals (lead, chromium, cadmium, etc.). There may be a problem that harmful substances and other impurities remain to adversely affect the skin.

The fibrous chitosan may have an average thickness within the range of 0.010 to 0.050 mm, preferably within the range of 0.015 to 0.040 mm. When the average thickness of the fibrous chitosan is less than 0.010 mm, the dissolution rate may be increased, but there may be a problem in that the handling and manufacturing of the fibrous form is complicated or difficult, and on the contrary, when the average thickness of the fibrous chitosan exceeds 0.050 mm, the dissolution rate There is a problem that the property of the fast solubility disappears late, and it becomes inconvenient to dissolve and use by the user immediately before use. Fibrous chitosan may be included in amounts within the range of 1.7 to 4.0 weight percent.

The chitosan may be powdery chitosan having an average thickness within the range of 0.150 to 0.450 mm, preferably within the range of 0.190 to 0.400 mm, more preferably within the range of 0.195 to 0.395 mm, powdery chitosan is 2.6 to 6.0 It may be included in an amount within the range of weight percent.

The fibrous chitosan can be obtained by dissolving chitosan in acid, stirring and processing by wet spinning. At this time, after the processing, washing and drying process, the used acid is removed and does not remain, so that the solubility of chitosan by the use of the acid in the form of salt increases.

The powdery chitosan can be obtained by grinding the chitosan, which also increases the solubility in the form of a salt.

The chitosan may have a dissolution rate dissolved in a solvent having a pH within the range of 3.5 to 7.5 within 1 to 15 minutes, preferably within the range of 5 to 10 minutes. If the dissolution rate is less than 1 minute, there is no particular problem, but in order to increase the dissolution rate requires a lot of effort and time in the production of chitosan, there may be a problem that the productivity is lowered, on the contrary exceeding 15 minutes immediately before use There may be a problem that it is inconvenient to dissolve and use by the user.

In addition, the above dissolution rate is generally adjusted to 10 to 15 minutes, which is a time range during which women wash face, and is intended to be mixed, dissolved, and used after cleansing before washing. In addition, the chitosan may be included in an amount within the range of 0.5 to 6.0% by weight, preferably 0.9 to 1.4% by weight based on the total weight of the cosmetic composition according to the present invention. When the content of the chitosan is less than 0.5% by weight, the chitosan solution obtained by dissolving the chitosan in the solvent is too low, the skin lifting effect is lowered, there may be a problem such as lowering the customer satisfaction On the contrary, if it exceeds 6.0% by weight, the viscosity of the obtained chitosan solution is too high, it is difficult or inconvenient to apply to the skin may also have a problem such as lowering the customer satisfaction.

The solvent is present in the cosmetic composition according to the present invention in a state separated from the chitosan and functions to facilitate the application of chitosan to the skin by dissolving the chitosan by the user immediately before use. Chitosan hardly decomposes or denatures at room temperature in the dried solid state, but once dissolved and in solution, it easily decomposes at room temperature, and molds grow well. However, the present invention is characterized in that the chitosan is kept in a dry solid state, thereby avoiding the use of a preservative. In addition, after dissolving in a solvent due to the nature of chitosan, the molecular structure decomposes over time, resulting in low molecular weight, and thus viscosity decreases with time, and the decrease in viscosity decreases the lifting effect when applied to the skin. However, in the present invention, the chitosan is kept in a dry solid state and dissolved in a solvent immediately before use, so that the chitosan solution having a high viscosity with almost no decomposition can be applied immediately to the skin. A cosmetic composition with excellent effect can be provided. The solvent may have a pH within the range of 3.5 to 7.5, preferably within the range of 5 to 7. If the pH of the solvent is less than 3.5, there is a problem that the acidity is too strong may cause irritation or damage to the skin, which is not suitable for cosmetic use, if it exceeds 7.5, chitosan is not dissolved or dissolution rate There may be a problem that is too slow to be dissolved by the user immediately before use is inconvenient to use.

Optionally, the water may be fresh water, rock water, deep sea water, mineral water, loess longevity, bamboo salt purified water, ginseng water, red ginseng water, ginseng fruit water, mineral water, carbonated water, germanium water, berry-containing water, mineral-containing water, and two or more of them. It may be selected from the group consisting of a mixture.

The cosmetics may be further added to various natural raw materials such as functional raw materials, such as arbutin, adenosine, green tea or green tea extract, depending on the characteristics of the product.

The cosmetic composition for skin lifting comprising a quick-release chitosan according to the present invention having the configuration features as described above comprises a solvent suitable for rapidly dissolving the chitosan with a quick-to-use property, and immediately before use. It allows the user to immediately use the chitosan after dissolving it in the solvent, and according to such configuration features, unlike conventional chitosan-containing cosmetics, it is stored after preparation without using synthetic additives such as preservatives and surfactants. It has the advantage of easy distribution and at the same time, it can be stored for a long time without including preservatives and surfactants, and there is no change in the physical properties of chitosan despite long-term storage, and the user dissolves the chitosan immediately before use. Synthesis of preservatives and surfactants It is possible to use the Hamchitosan cosmetic composition which can maintain fresh and original efficacy without suffering from the harmful effects of the false substances, especially by applying chitosan solution that shows high viscosity to the skin immediately after dissolving chitosan in a solvent. By application, skin lifting effects can be expected in particular.

Hereinafter, the present invention will be described in more detail with reference to Examples. These embodiments are only for explaining the present invention in more detail, and the scope of the present invention is not limited by these embodiments in accordance with the gist of the present invention, those skilled in the art. It will be obvious to

Experimental Example 1: Solubility test according to the thickness of chitosan

Powdered chitosan or fibrous chitosan (prepared using chitosan only) with the same degree of deacetylation (90%) except that each had a different average thickness until solids were not visible or further dissolved The dissolution time was measured using the time until no change was seen as the dissolution time. The solvent used was acidic water of pH 4 obtained by dissolving glycolic acid in water (1.2 wt% fibrous chitosan, 98.8 wt% acidic water), and the instrument used for measuring the thickness of fibrous chitosan was manufactured by e Scope Professional PRO (manufactured by Company name: OiTEZ Co., Ltd. in China, and the magnification was 500 times.

Fibrous chitosan of FIG. 1 had an average thickness of 0.0153 mm, and a dissolution time was about 7 minutes.

Fibrous chitosan in FIG. 2 had an average thickness of 0.0215 mm, and the dissolution time was about 9 minutes.

Fibrous chitosan in FIG. 3 had an average thickness of 0.03979 mm, and the dissolution time was about 13 minutes.

Fibrous chitosan of FIG. 4 had an average thickness of 0.2697 mm and was found to be impossible to dissolve completely within 1 hour.

The powdery chitosan of FIG. 5 was found to have an average particle diameter of 0.332386 mm, 0.195323 mm and 0.350297 mm, respectively.

The powdery chitosan of FIG. 6 was found to have an average particle diameter of 0.296869 mm, 0.390526 mm and 0.222697 mm, respectively.

Experimental Example 2: Solubility test according to deacetylation degree of fibrous chitosan

Solubility was measured using fibrous yarns (manufactured using chitosan only) having the same thickness (0.025 mm) except that each had a different degree of deacetylation. The solvent used was acidic water of pH 4 obtained by dissolving glycolic acid in water (1.2% by weight of fibrous chitosan, 98.8% by weight of acidic water), and the results of the solubility experiment are shown in the photograph of FIG. 7.

As shown in FIG. 7, it was confirmed that the solubility increased with increasing deacetylation degree. In particular, in the case of deacetylation degree of 70% and 75%, it was confirmed that the solubility was so low that the cosmetically solid content that was difficult to apply to the skin was largely visible to the naked eye, whereas in 80% to 90%, the solid content was visually It showed solubility that can be used for the purpose of this show or cosmetics, and it was confirmed that at 90% or more, the undissolved solids exhibited high solubility that cannot be seen with the naked eye.

Experimental Example 3: Test for measuring the physical properties of the obtained chitosan solution and dissolution time according to the content of fibrous chitosan and powdery chitosan prepared from chitosan

Based on Experimental Examples 1 and 2, powdery chitosan and fibrous chitosan were prepared using chitosan having a deacetylation degree of 95%, and the physical properties of the chitosan solution obtained after solubility and dissolution were measured. 1-1 to Table 1-6). At this time, the powdery chitosan was an average particle diameter of 0.298mm, the fibrous chitosan was an average thickness of 0.025mm, and the acidic water of pH 4 adjusted to pH with purified water or glycolic acid was used as a solvent. The measurement of the viscosity was performed at room temperature (25 ° C). In Table 1, chitosan insoluble means that it is insoluble in neutral water and dissolved in acidic water (glycolic acid is used as AHA acid), and the powder is powdered, and the yarn is made in fibrous form. In addition, in the following Table 1, chitosan water solubility means that it is soluble in neutral water, and the powder is powder, meaning that the yarn is made of fibrous.

Table 1-1

TABLE 1-2

Table 1-3

Table 1-4

Table 1-5

Table 1-6

In relation to the dissolution time of the experimental results shown in Table 1, it is assumed that the cosmetics are used under ordinary conditions and do not control the temperature, and are believed to be due to the measurement at a random temperature. No measurement error was identified. Although the dissolution time was somewhat irregular, both powdery chitosan and fibrous chitosan showed a tendency to increase as the overall input amount increased, but it was confirmed that the dissolution time of fibrous chitosan further increased. This is considered to be due to the difference in surface area and the like depending on the shape.

Considering some measurement errors in relation to the pH of the experimental results shown in Table 1, the pH tends to decrease consistently with the increase in the amount of powdery chitosan and fibrous chitosan, but less decrease in pH in the fibrous chitosan. It can be seen that the tendency to decrease.

Among the experimental results shown in Table 1 above, in particular, in relation to the viscosity closely related to the purpose of the present invention, both the powdery chitosan and the fibrous chitosan showed a tendency to increase the viscosity consistently with the increase in the amount, in particular according to the present invention. Fibrous chitosan showed a higher viscosity, and as the dosage increased, fibrous chitosan showed a much greater increase in viscosity compared to powdery chitosan.

Experimental Example 4: Storage test of chitosan solution

The change in storing the chitosan solution obtained by dissolving 1.2% by weight of chitosan having a deacetylation degree of 95% as used in the above experimental examples in 98.8% purified water was tested.

As shown in FIG. 8, when the chitosan solution was refrigerated (average refrigeration temperature: 4 to -5 ° C.), fungi did not occur even after 30 days of refrigeration, but as shown in FIG. Temperature: 25 ℃) In the case of storage, it was confirmed that the fungus bacteria grow and grow after 10 days at room temperature storage.

Experimental Example 5: Sensory Test Related to Age Preference

Prepare chitosan solutions by concentration of 0.5% to 2.0% by weight (the remaining amount is acidic water used in the above experiments), and adult women from 20s to 50s by age group (ie, 20s and 30s). , 40's and 50's) consisted of a total of 120 panels of 30 people, and each panel was provided with chitosan solutions for each concentration (total 16 species), and then randomly used for 1 month, and then 1 month. The preference sensory test was performed to check the chitosan solution of the most preferred concentration at the elapsed time point, and the results are shown in Table 2 below. For the fairness of the experiment, the chitosan solutions for each concentration were prepared by increasing the concentration of 0.5 to 0.1 wt% in the range of 0.5 wt% to 2.0 wt%, and the prepared chitosan solutions were prepared at low concentration and high concentration. English alphabets (A to P) were sequentially assigned to the panel and provided to the panel.

TABLE 2

As shown in Table 2, the chitosan concentration within the range of 0.9% to 1.3% was high overall preference, in particular within the range of 1.0% to 1.2% was relatively higher, 1.1% and 1.2% relative The concentration was very high, and among them, the concentration of 1.2% was the most preferred. Especially, the concentration of 1.1% by weight was evenly high in the 20s and 40s, but the relative 1.2% by weight was especially high in the 40s and 50s. .

Experimental Example 6: Lifting Effect Test

Based on the above experimental examples, a fibrous chitosan having an average thickness of 0.025 mm was prepared using chitosan having a deacetylation degree of 95%, and glycolic acid was dissolved in purified water to prepare acidic water having a pH of 4, and the acidic water A cosmetic composition was prepared by dissolving 1.2% by weight of the fibrous chitosan in 98.8% by weight, which was used for a lifting effect experiment.

Lifting effect experiments were performed as follows.

Test Period: January 08, 2015-January 23, 2015

Test product: cosmetic composition of the above description

Purpose: To evaluate the effects of eyelid lifting, mouth lifting, facial sagging, nasolabial folds, neck wrinkles, moisture content, and keratin improvement after using the cosmetic composition described above once and twice a week

Subjects: Women aged 20 to 55 who meet the subject selection and exclusion criteria

Number of subjects: 21

Subject Selection Criteria: Those who meet the following criteria

1) Adult women aged 20 to 55

2) A person who has fully explained the purpose and contents of the examination and voluntarily signed the consent

3) A person who can follow up during the trial period

4) Those who are not included in the subject exclusion criteria

Exclusion Criteria for Subject Selection: Any one of the following exclusion criteria cannot participate in this test.

1) If you do not want or do not complete the consent form

2) If you have a psychiatric illness

3) Infectious skin disease

4) In case of receiving immunosuppressive treatment within 3 months of participation

5) If systemic steroid or phototherapy was received within 1 month of participation

6) Difficult to measure due to lesion on test site

7) If you have atopic skin

8) Severe or allergic reactions to cosmetics, medicine or routine light exposure

9) If you have skin skating or skin care within 3 months of participating

10) pregnant or lactating

11) In addition to the above, if the responsible researcher or the person in charge of the test determines that it is difficult to perform the human application test.

Test Method: Subjects who agreed to participate in the human body application test and met the selection criteria and exclusion criteria were to use the test product for 2 weeks. Subjects used the test product at visit 1 and evaluated the test site before and immediately after using the product. The same test site was evaluated two weeks after use of the product at visit 2, and the validity and preference questionnaire evaluation was completed after the end of the test.

Evaluation item:

1) Mechanical evaluation

-Skin (eye, mouth, cheek) lifting measurement (F-ray)

-Skin Pleated Wrinkle Measurement (Primos Pico)

-Skin neck wrinkle measurement (Primos Pico)

-Skin moisture content measurement (Corneometer)

Skin keratin measurement (Visioscan VC98)

2) Global Assessment of Efficacy: Subject Evaluation

3) Safety evaluation: The researcher evaluates the results of the researcher's visual evaluation and the subject's questionnaire on the site of the test product used at each visit.

Other survey items:

1) Demographic survey: Survey of gender, date of birth and age before the start of human testing

2) Vital sign investigation: physical examination through visual evaluation before starting the human application test

3) Medical history investigation: Investigate the main symptoms, the date of onset, examination and treatment history before the start of the human testing.

4) Product symbol survey: Survey of product symbol through survey data

Visited: 2 visits

1) Visit 1: Subject acceptance, subject selection inspection, measurement before and after use of product, product distribution

2) Visit 2: Confirmation of adverse reaction, measurement after 2 weeks of product use, survey evaluation

Primary efficacy evaluation variables: measurement of skin crow's feet, mouth lifting, ball lifting, nasolabial folds, neck wrinkles, moisture content, and angular mass before and after using the test product

Secondary Validation Variables: Results of the Global Assessment of Efficacy

Safety Assessment: Evaluate abnormal symptoms after use of the test product.

Evaluation site and measuring method: For mechanical evaluation, the test subject is allowed to settle for 30 minutes in an air-conditioned room with a room temperature of 20 to 25 and a humidity of 40 to 60% to adjust the skin surface temperature and humidity to the environment of the measurement space. During rest, water intake was limited. One investigator was measured for objective measurement, and the same area was measured at each measurement.

Skin Lifting Measurement: Skin lifting measurement was performed by analyzing the image of the eyes, cheeks and mouth area using Image-pro plus for images taken using F-ray, a device that expresses the appearance of the skin in contours.

The tail lifting was evaluated by drawing a straight line based on the tip of the tail and measuring the angle with the line across the tip of the tail. As the angle between the two straight lines increases, it can be seen that there is an eye tail lifting effect.

Facial lifting was evaluated by drawing a straight line in the center of the circle based on the circle formed near the cheekbone and measuring the angle with the straight line across the mouth. It can be seen that the smaller the angle between the two straight lines has the ball lifting effect.

Mouth lifting was evaluated by drawing a straight line based on the tip of the mouth and measuring the angle with the line drawn from the center of the circle based on the circle formed near the tip of the mouth and the cheekbones. As the angle between the two straight lines increases, it can be seen that there is a particle lifting effect.

Skin-palm wrinkle measurement: The skin-palm wrinkle measurement was taken on the same area in the palm area before and immediately after using the test product using the 3D skin imaging device (Primos Pico), and after 2 weeks, Ra (mean roughness parameter) of the skin wrinkle parameter of the stored image. : average roughness) value was analyzed.

Skin Neck Wrinkle Measurement: Skin neck wrinkle measurement was performed using 3D skin imaging device (Primos Pico) before and immediately after use of the test product, two weeks after the same part of the neck was taken, and the skin wrinkle parameter Ra (average roughness) of the stored image. : average roughness) value was analyzed.

Skin moisture content measurement: Skin moisture content measurement was measured using Corneometer CM825 (Courage-Khazaka electronic GmbH, Germany) before and immediately after use of the test product and two weeks after use. The measurement was performed three times through the sensor by contacting the corneometer probe with the skin, and the average value was used as an evaluation data for skin moisture content. The corneometer can measure the moisture content by measuring the capacitance at the point of contact of the probe. The unit is A.U, which is a unit constant, and the skin moisturization and the measured value are proportional.

Skin keratin measurement: The skin keratin measurement was measured before and immediately after using the test product and two weeks after using Visioscan VC98 (Courage-Khazaka eletronic GmbH, Germany). The measurement was performed by using a special film (Corneofix), and after taking the keratin of the cheek area, images were taken and analyzed by Visioscan VC98, and the measured value DI (Desquamation Index) was used as the evaluation data of the skin keratin. Calculated using.

Where

D.I is the epidermal loss index (%), A is the percentage of area covered with keratinocytes, Tn is the percentage of keratinocytes associated with thickness, and n is the level of thickness (1-5).

Global Assessment of Efficacy: Very good (4), good (3) for the degree of eyelid lifting, mouth lifting, facial sag (ball lifting), nasolabial folds, neck wrinkles, moisture content, and keratin improvement after using the test product ), Moderate (2), bad (1), very bad (0) in five steps to answer the questionnaire directly. The investigator determined the efficacy of the test product by obtaining the percentage of the subjects for each answer.

Safety evaluation: The safety of the test product was calculated by integrating the identified adverse reactions among all subjects using the test product and all reported adverse reactions during the test period, and used as the safety evaluation data of the product.

Product preference survey evaluation: The test subjects were asked to answer the questionnaire directly about the product feeling after using the test product. The evaluation items were examined on skin moistness, smoothness, softness, spreadability, scent, and overall feeling of use, and were evaluated as good (4), good (3), moderate (2), bad (1), and bad (0). It was evaluated in five stages.

Adverse reaction evaluation: Abnormal skin symptoms occurred during the use of the test product were examined during the test period to determine the occurrence and symptoms. If the subject felt abnormal, he / she was instructed to report it immediately. The investigator informed the responsible investigator when an adverse reaction was reported, and the lead investigator judged the extent of the symptom, the association with the test product, and determined the appropriate measures for the symptom and participation in the test.

Evaluation standard:

Primary efficacy parameters: The primary efficacy parameters of the test product are measured before and after use of the test product, including skin moisture content, wrinkles (forehead, eyes, and limbs), pores, blemishes, lifting, fastness, resilience, and brown spot measurements. It evaluated on the basis.

Secondary efficacy evaluation variables: Secondary efficacy evaluation variables of the test product were evaluated based on the results of the efficacy survey for skin improvement effects after using the test product.

Statistical analysis: We used SPSS 19.0, a statistical analysis program, to determine the significance of the measured values before using the test product. In the 95% confidence interval, the significance was confirmed when the significance probability was p <0.05, and the significance probability was expressed by rounding to the third decimal place. Continuous variables are summarized as mean and standard deviation, and categorical variables as frequency and percentage. Repeated measurements were repeated three times or more using the repeated measures ANOVA as the normality test parametric method and post-tested by the Bonferroni method. Nonparametric methods were compared by the Friedman test, Wilcoxon signed rank test, and then post-tested by correcting the significance level with the Bonferroni method.

Experimental results are shown in Fig. 10 (lifting the tail), Fig. 11 (lifting the mouth), Fig. 12 (ball lifting), Fig. 13 (improved nasolabial folds), Fig. 14 (improved neck wrinkles), Fig. 15 (skin moisture content) and Fig. 16 (Skin improvement).

As shown in Figure 10, in order to confirm the change of the tail lifting value by the use of the test product, the tail tail lifting angle before and after two weeks of use was measured. The tail lifting angle measurement was 46.08 ± 10.42 before use, 46.78 ± 10.56 immediately after use, and 48.10 ± 10.33 after 2 weeks of use. Changes in skin lifting angle were tested for statistical significance. As a result of the test by post hoc Bonferroni correction, which is a parametric method according to the normality test, it was confirmed that the tail lifting angle increased significantly (p <0.05) immediately after use and after 2 weeks compared to before use. there was.

As shown in Figure 11, in order to confirm the change in the value of the lifting value by the use of the test product was measured before the use, 2 weeks after use. The value of the mouth lifting angle was 41.1 ± 17.18 before using the test product, 41.70 ± 7.07 immediately after use, and 42.90 ± 7.17 after 2 weeks of use. Changes in skin lifting angle were tested for statistical significance. According to the normalized test, repeated measures ANOVA (post hoc Bonferroni correction), which is a parametric method, showed that the mouth lifting angle increased significantly (p <0.05) immediately after and 2 weeks after use. .

As shown in FIG. 12, the ball lifting angle was measured before use and 2 weeks after use in order to confirm the change of the ball lifting value by using the test product. The ball lifting angle measurement was 36.34 ± 7.40 before using the test product, 35.74 ± 7.33 immediately after using, and 34.95 ± 7.34 after 2 weeks of using. Changes in skin lifting angle were tested for statistical significance. According to the normalized test, the repeated measures ANOVA (post hoc Bonferroni correction), a parametric method, showed that the ball lifting angle was significantly decreased (p <0.05) immediately after and 2 weeks after use. .

As shown in FIG. 13, in order to confirm the change in the nasolabial folds caused by the use of the test product, the nasolabial folds were measured before use and two weeks after use. The nasolabial fold measurements were 19.87 ± 2.96 before using the test product, 18.76 ± 2.64 immediately after using the test product, and 18.32 ± 2.86 after 2 weeks of using the test product. The change of skin palsy was statistically tested for statistical significance. According to the normality test, the non-parametric Friedman test (post hoc Wilcoxon signed rank test with Bonferroni correction) showed that the nasolabial folds were significantly reduced (p <0.025) immediately after and 2 weeks after use. I could confirm it.

As shown in FIG. 14, the neck wrinkles were measured before use and two weeks after use in order to confirm the change of the neck wrinkles caused by the use of the test product. The measured neck wrinkles were 23.88 ± 2.75 before use, 22.44 ± 3.19 immediately after use, and 21.79 ± 2.88 after 2 weeks of use. The change in skin wrinkles was tested by statistical method. According to the normalized test, repeated measures ANOVA (post hoc Bonferroni correction), a parametric method, showed that the neck angle decreased significantly (p <0.05) immediately after and 2 weeks after use. .

As shown in Figure 15, in order to confirm the change in the water content value by the use of the test product, the skin water content before and after two weeks of use was measured. Skin moisture content was 61.48 ± 9.33 before using test product, 82.79 ± 8.48 immediately after using, and 71.96 ± 7.56 after 2 weeks of using. Changes in skin moisture content were tested for statistical significance. According to the normalized test, repeated measures ANOVA (post hoc Bonferroni correction), which is a parametric method, showed that the water content increased significantly (p <0.05) immediately after and 2 weeks after use.

In addition, as shown in Fig. 16, in order to confirm the change of the keratin level by the use of the test product, the skin angular mass after use and two weeks after the use was measured. Skin keratin measurements were 19.59 ± 2.59 before test product use, 13.60 ± 2.82 immediately after use, and 15.48 ± 2.61 after 2 weeks of use. The change in keratin angle of skin was statistically tested for significance. According to the normality test, the non-parametric method, Friedman test (post hoc Wilcoxon signed rank test with Bonferroni correction), showed that the skin keratin significantly decreased (p <0.025) immediately after and 2 weeks after use. I could confirm it.

Efficacy Assessment Survey Results: A survey on the questionnaire values, standard deviations, and answers in the survey conducted on the degree of effect of eye tail lifting, mouth lifting, facial sagging (ball lifting), nasolabial folds, neck wrinkles, moisture, and keratin improvement after using the test product. The percentage of the subjects was obtained and the results are shown in Table 3 below.

TABLE 3

As a result of the questionnaire evaluation, more than 90% of the subjects evaluated the degree of improvement of eye tail lifting, mouth lifting, facial sagging (ball lifting), nasolabial folds, neck wrinkles, moisture content, and keratin improvement.

Results of Survey on Product Acceptance Survey: A survey was conducted after the use of the product to examine the degree of acceptance of the test subjects regarding the skin's moisture, smoothness, softness, spreadability, aroma, and overall feeling of use. The survey results were expressed as the average standard deviation of the answers for each item, the results are shown in Table 4 below.

TABLE 4

As a result, more than 85% of the test subjects evaluated the skin moisture, smoothness, softness, spreadability, fragrance, and overall feeling of use of the test product as above average.

Test Results: In this study, the test product was used for women aged 20 to 55 years once and for 2 weeks before and immediately after using the product, and after 2 weeks. Lifting, nasolabial folds, neck wrinkles, moisture content, and keratin were measured. After the completion of the test, the subjects' survey was completed, and the following conclusions were obtained.

1) The 21 subjects who participated in this study were all women, and the average age was 40.05 years. The selected subjects had no specific skin symptoms and no history of disease or medication that could affect the study.

2) As a result of measuring the tail of the eye after using the test product, the angle measurement value increased significantly (p <0.05) immediately after the use and after 2 weeks.

3) After the use of the test product, the value of the grain size lifting was measured. As a result, the angle measurement value increased significantly (p <0.05) immediately after use and 2 weeks later.

4) As a result of measuring facial lifting after using the test product, the angle measurement value was significantly decreased (p <0.05) immediately after use and after 2 weeks.

5) As a result of measuring the size of the nasolabial fold after using the test product, the measured value of the nasolabial fold decreased immediately after use and 2 weeks after the use (p <0.025).

6) As a result of measuring neck wrinkles after using test product, neck wrinkles measured value decreased significantly (p <0.05) immediately after use and after 2 weeks.

7) As a result of measuring the water content after using the test product, the measured water content increased significantly (p <0.05) both immediately after use and after 2 weeks.

8) As a result of measuring keratin after using the test product, the measured angular mass was significantly decreased (p <0.025) immediately after use and 2 weeks later.

9) The results of the survey on the effectiveness of the test product showed that more than 90% of the test subjects rated the eye tail lifting, the mouth lifting, the face (ball) lifting, the nasolabial folds, the neck wrinkles, the moisture content, and the keratin improvement effect. .

10) There were no reports of adverse reactions during the use of this test product by 21 subjects, and no skin abnormalities such as erythema, rash and pruritus were observed by the dermatologist.

Therefore, the cosmetic composition according to the present invention provides immediate eye tail lifting improvement, mouth lifting improvement, facial sag (ball lifting) improvement, nasolabial fold improvement, neck wrinkle improvement, moisture content improvement, keratin improvement by two-time use, and use for two weeks. It is believed to be a product that helps to improve eye tail lifting, mouth lifting, facial sagging (ball lifting), nasolabial folds, neck wrinkles, moisture, and keratin.

The present invention can be used in the field of industry.

Claims

Chitosan 0.5 having rapid solubility that dissolves in a solvent having a degree of deacetylation (DA) within the range of 80 to 100% and a pH within the range of 3.5 to 7.5 at a dissolution rate within the range of 1 to 15 minutes. To 6.0 weight percent; And a solvent having a pH within a range of 3.5 to 7.5 as a residual amount, wherein the chitosan is physically separated from the solvent and is not dissolved. Cosmetic makeup composition.
The method of claim 1,

The cosmetic composition for skin lifting comprising chitosan, characterized in that the chitosan has a degree of deacetylation within the range of 90 to 100%.
The method according to claim 1 or 2,

The chitosan may be fibrous chitosan having a thickness within the range of 0.010 to 0.050 mm, and the fibrous chitosan is included in an amount within the range of 1.7 to 4.0% by weight for skin lifting, including chitosan having rapid solubility. Cosmetic composition.
The method according to claim 1 or 2,

The chitosan may be a powdered chitosan having an average thickness within the range of 0.150 to 0.450mm, the powdered chitosan is contained in an amount within the range of 2.6 to 6.0% by weight skin lifting comprising chitosan having a quick solubility Cosmetic makeup composition.
The method of claim 1,

The solvent is water; Or glycolic acid, lactic acid, lactic acid, citric acid, malic acid, tartaric acid, citric acid, ascorbic acid (vitamin C), ascorbyl phosphate, ascorbyl glucoside, magnesium ascorbyl phosphate, sodium ascorbyl phosphate, ascorbic A cosmetic composition for skin lifting comprising chitosan having a rapid solubility, wherein the pH adjuster selected from the group consisting of bilpalmitate and a mixture of two or more thereof is dissolved.