KR101596634B1

KR101596634B1 - Cosmetic composition comprising chitosan having rapid melting properties for skin lifting

Info

Publication number: KR101596634B1
Application number: KR1020150067714A
Authority: KR
Inventors: 손미경; 박순영
Original assignee: 손미경; 박순영
Priority date: 2014-05-14
Filing date: 2015-05-14
Publication date: 2016-02-25
Also published as: KR101850053B1; KR20150132039A; WO2015174772A1; WO2015174772A9; KR20140113614A; KR20150132011A

Abstract

The present invention does not include a preservative, a surfactant, etc., which is free of chitosan and has a readily available chitosan in an unheated state and can be buried or adversely affected by the use of the chitosan by dissolving it by a user immediately before use, To a cosmetic composition suitable for use in cosmetics having a degree of deacetylation (DA: Degree of Deacetylation) in the range of 80 to 100% and a solubility in a solvent having a pH in the range of 3.5 to 7.5 at a dissolution rate in the range of 1 to 15 minutes 0.5 to 6.0% by weight of chitosan having high solubility; And a solvent having a pH within a range of 3.5 to 7.5 as the balance, wherein the chitosan is physically separated from the solvent and is not dissolved.

Description

TECHNICAL FIELD [0001] The present invention relates to a cosmetic composition for skin lifting comprising chitosan having high solubility,

The present invention relates to a cosmetic composition for skin lifting comprising chitosan having high solubility, and more particularly to a cosmetic composition for skin lifting comprising chitosan having high solubility in an unheated state and dissolving it by a user immediately before use, Which does not contain preservatives, surfactants and the like, which are capable of imparting a desired effect to skin, and which is suitable for skin lifting.

The problem of environmental pollution becomes serious and the number of people looking for cosmetics of natural raw materials is increasing because of skin trouble. In addition, interest in well-being has increased, and natural raw materials are naturally getting into place at the beginning of the well-being lifestyle law due to raw material characteristics. Natural is the central concept of everlasting cosmetics, and how to improve and advance it is a challenge.

The cosmetics market has a three-dimensional market structure with a two-dimensional structure of 'natural' and 'function' plus a vertical axis of 'health'. Each element of natural, function, and health is reinforced through fusion or simplicity in terms of beauty. These 'healthy beauties' should start with taking the worse out of adding good things. No matter how many good ingredients are present, if the various chemical components coexist for their fusion, they will never bring good results to the skin.

As cosmetics are not medicines, it is important to use safer ingredients, more fresh products, and more functional products that are safer to the skin than immediate effects, and the desire of these consumers is already becoming a global trend . The risk of chemical preservative, which is essentially contained in the expiration date of one to two years and reuse due to capacity, is an inevitable problem for cosmetics users, and many consumers are experiencing skin troubles. In order to escape the risk of these chemical preservatives, the number of consumers who make and use natural cosmetics themselves is increasing every year.

Accordingly, there is a demand for the development of instant cosmetics that uses the safest materials on the skin and the properties of the raw materials to produce cosmetics on the spot and apply the efficacy of the raw materials to the skin freshest.

The cosmetics currently on the market are being released as a finished product with different functions and performance by mass production methods which are collectively made in the factory. This is because, under the domestic Cosmetics Act, only the manufacturer who has a certain manufacturing facility and quality control facility and is authorized by the authorities can manufacture cosmetics.

These factory mass production methods are easy to monitor each process so that consumers can safely consume them, have strict quality control, and are advantageous in terms of manufacturer's follow-up or follow-up management for side effects or accidents caused by cosmetics consumption.

However, these mass production methods do not satisfy consumer 's diverse needs due to the characteristics of standardization, standardization and mass production, and it is difficult to activate personalized cosmetics market because they can not actively respond to various demands of consumers.

In addition, cosmetics produced unilaterally according to the needs of the company are supplied regardless of consumers' needs, consumption purpose, and taste.

For example, since the second material contained in one cosmetic product is the same, but merely added with merits or functional materials, it is displayed on the market as another product, so that the consumer can enjoy cosmetics having the same second material, It is often necessary to purchase a new one.

As a result, consumers are increasingly manufacturing cosmetics using natural materials directly at home.

On the other hand, chitosan is a natural animal marine polymer having a unique cation in nature and has affinity with human body, has no rejection reaction, no side effects, has no irritation and is a biosurfactant substance and acts on skin to exfoliate, Activity, skin barrier formation, and adsorption of impurities. However, up to now, it has been recognized and used only as a health food that is simply ingested, and development as a cosmetic useful for skin beauty is insufficient.

Disclosure of Invention Technical Problem [8] Accordingly, the present invention has been made to solve the above problems of the prior art, and it is an object of the present invention to provide a chitosan having an easy-to-use state in an undissolved state, And does not contain preservatives, surfactants, etc., and provides a cosmetic composition suitable for skin lifting.

The cosmetic composition for skin lifting comprising the readily soluble chitosan according to the present invention is characterized in that it has a degree of deacetylation (DA: Deacetylation) within a range of 80 to 100% and a solvent having a pH within a range of 3.5 to 7.5 0.5 to 6.0% by weight of a readily available chitosan dissolved at a dissolution rate within a range of 1 to 15 minutes; And a solvent having a pH within a range of 3.5 to 7.5 as the balance, wherein the chitosan is physically separated from the solvent and is not dissolved.

The chitosan may have a degree of deacetylation preferably within a range of 90 to 100%, more preferably within a range of 95 to 100%.

The chitosan may be a fibrous chitosan having an average thickness within a range of 0.010 to 0.050 mm and the fibrous chitosan may be contained in an amount within a range of 1.7 to 4.0 wt%.

The chitosan may be powdery chitosan having an average thickness within a range of 0.150 to 0.450 mm, and the powdery chitosan may be contained in an amount within a range of 2.6 to 6.0 wt%.

The solvent may be water; (Vitamin C), ascorbyl phosphate, ascorbyl glucoside, magnesium ascorbyl phosphate, sodium ascorbyl phosphate, ascorbyl phosphate, ascorbyl palmitate, ascorbyl palmitate, ascorbyl palmitate, ascorbyl palmitate, And a pH adjuster dissolved in an acidic aqueous solution selected from the group consisting of a mixture of two or more thereof.

According to the present invention, chitosan having an easy solubility in an unheated state is dissolved and used by a user immediately before use, so that it does not contain a preservative and a surfactant that can give burden or adverse effects to the skin, It is possible to prevent the risk of chemical preservatives, especially skin troubles, which are pointed out as problems in the prior art by providing an appropriate cosmetic composition for cosmetics, And the effect of the raw material can be most freshly applied to the skin.

Figs. 1 to 6 are photographs of fibrous chitosan used in the solubility test according to the thickness of chitosan, particularly fibrous chitosan and powder chitosan used in the present invention.

Hereinafter, the present invention will be described in detail with reference to specific examples.

The term " chitosan " in the present invention is defined as meaning chitosan which is dissolved in acidic water obtained by including an acidic substance in water, and / or a pH within an acidic range and based on water.

Chitosan as a raw material of chitosan is a deacetylated product obtained by deacetylation of chitin obtained from shell of crustacean and is dissolved in water, especially weak acid, and is well absorbed in human body. And serves as a component that imparts a skin-lifting effect when applied to the skin. Chitosan is a glucosamine polymer that is contained in crustaceans such as shrimp, crab, bark of insects, and squid bones and obtained by alkali treatment of chitin. The main component is polyglucosamine, which is a colorless amorphous powder with a polymer structure. Chitosan, which is a glucosamine bond, is a very valuable biomaterial because it has a very similar molecular structure to that of human tissue and has very good affinity for human body and does not cause an immune reaction. The degree of deacetylation of chitosan can be measured using a colloid titration as described in K. Toei and T. Kohara, Analytica Chimica Acta, 83, 59-65 (1976).

The chitosan may have a deacetylation degree within a range of 80 to 100%, preferably within a range of 90 to 100%, more preferably within a range of 95 to 100%. When the degree of deacetylation is less than 80%, not only the solubility of chitosan is lowered but also the amount of remaining solid matter becomes unfavorably increased, which is unsuitable for application or application to the skin, and it is difficult to apply such as heavy metals (lead, chromium, cadmium etc.) Pests and other impurities may remain, which may adversely affect the skin.

The fibrous chitosan may have an average thickness within a range of 0.010 to 0.050 mm, preferably within a range of 0.015 to 0.040 mm. When the average thickness of the fibrous chitosan is less than 0.010 mm, the dissolution rate may be increased, but there may be a problem that the preparation and handling of the fibrous phase are complicated or difficult. On the other hand, There is a problem that the property of quick availability disappears and it is inconvenient to use it by the user just before use. The fibrous chitosan may be included in an amount within the range of 1.7 to 4.0 wt%.

The chitosan may be a powdery chitosan having an average thickness within a range of 0.150 to 0.450 mm, preferably within a range of 0.190 to 0.400 mm, more preferably within a range of 0.195 to 0.395 mm, and the powdery chitosan may be 2.6 to 6.0 % &Lt; / RTI > by weight.

The fibrous chitosan can be obtained by dissolving chitosan in an acid, stirring, and then wet spinning. At this time, after processing, washing and drying process, the used acid is not removed and remains, and the use of acid causes the chitosan to be in the form of a salt, thereby increasing the solubility.

The powdery chitosan can be obtained by pulverizing chitosan and also has increased solubility in the form of a salt.

The chitosan may have a dissolution rate that is dissolved in a solvent having a pH within the range of 3.5 to 7.5 within a range of 1 to 15 minutes, preferably within a range of 5 to 10 minutes. When the dissolution rate is less than 1 minute, there is no particular problem, but a lot of efforts and time are required to manufacture chitosan in order to increase the dissolution rate, and productivity may be lowered. Conversely, There is a problem that it is inconvenient to dissolve and use by the user.

In addition, the above-mentioned dissolution rate is generally adjusted to 10 to 15 minutes, which is a time range in which a woman performs cleansing, and is intended to be used before cleansing, dissolving, and after cleansing. The chitosan may be contained in an amount within a range of 0.5 to 6.0 wt%, preferably 0.9 to 1.4 wt%, based on the total weight of the cosmetic composition according to the present invention. When the content of the chitosan is less than 0.5% by weight, the viscosity of the chitosan solution obtained by dissolving the chitosan in the solvent may be too low to lower the skin lifting effect and lower the satisfaction of the consumer , On the other hand, when the amount of the chitosan solution is more than 6.0% by weight, the viscosity of the obtained chitosan solution is too high to be applied to the skin, which is inconvenient, and the satisfaction of the consumer is also lowered.

The solvent is present in a state separated from the chitosan in the cosmetic composition according to the present invention, and functions to dissolve the chitosan by a user immediately before use to easily apply chitosan to the skin. Chitosan is not nearly decayed or denatured at room temperature in a dry solid state. However, once it is dissolved and becomes a solution state, it becomes easily corroded at room temperature and is in a state where it is easy to grow mold. Therefore, in case of liquid chitosan product, However, in the present invention, chitosan is kept in a dry solid state so that use of preservatives can be avoided. Further, after dissolving in a solvent due to the characteristics of chitosan, the molecular structure is decomposed with time to lower the molecular weight, so that the viscosity of the chitosan is lowered with the lapse of time. In the present invention, chitosan is kept in a dry solid state and dissolved in a solvent immediately before use. Thus, it is possible to apply a chitosan solution having a high viscosity almost free from decomposition to the skin immediately, A cosmetic composition having an excellent effect can be provided. The solvent may have a pH within the range of 3.5 to 7.5, preferably within the range of 5 to 7. [ When the pH of the solvent is less than 3.5, the acidity is too strong, which may cause irritation or damage to the skin, making it unsuitable for cosmetic use. If it exceeds 7.5, the chitosan will not dissolve or the dissolution rate May be too slow to be dissolved by the user immediately before use, which makes it inconvenient to use.

Alternatively, the water may be at least one selected from the group consisting of furoral water, mineral water, deep sea water, mineral water, yellow clay water, bamboo salt purified water, ginseng water, red ginseng water, ginseng fruit water, mineral water, carbonated water, germanium water, And mixtures thereof.

The cosmetic may further contain various natural raw materials such as arbutin, adenosine, etc., functional raw materials, green tea or green tea extract depending on the characteristics of the product.

The cosmetic composition for skin lifting comprising the readily available chitosan according to the present invention having the above-described constitutional features comprises a solvent suitable for rapidly dissolving the chitosan having quick-drying properties, It is possible for the user to use the chitosan immediately after dissolving the chitosan in the solvent, and unlike conventional cosmetic preparations containing chitosan, according to the characteristics of such constitution, it is possible to use a cosmetic composition containing preservatives and surfactants It is possible to maintain long-term storage without preservatives and surfactants, and it has no change in the physical properties of chitosan despite long-term storage, and the user can dissolve the chitosan immediately before use , And it is possible to use it immediately, so that the synthesis of preservatives and surfactants A chitosan cosmetic composition capable of maintaining fresh and original efficacy without suffering from harmfulness of garbage can be used for skin, and in particular, a chitosan solution showing high viscosity immediately after dissolving chitosan in a solvent is applied to skin The skin lifting effect can be expected particularly.

Hereinafter, the present invention will be described in more detail with reference to Examples. It is to be understood, however, that these embodiments are provided for illustrative purposes only and that the scope of the present invention is not limited by these embodiments in accordance with the principles of the present invention, It will be obvious to you.

Experimental Example 1: Solubility test according to thickness of chitosan

Using powdered chitosan or fibrous chitosan (prepared using only chitosan) having the same degree of deacetylation (90%), except that each has an average thickness different from each other, the solid content is visually invisible or in a further dissolved state The dissolution time was measured as the dissolution time until the time when no change was observed. The solvent used was acidic water having a pH of 4 (1.2 wt% of fibrous chitosan and 98.8 wt% of acidic water) obtained by dissolving glycolic acid in water. The apparatus used for measuring the thickness of fibrous chitosan was e Scope Professional PRO Company name: OiTeZ Co., Ltd., China), and the magnification was 500 times.

The fibrous chitosan of Figure 1 had an average thickness of 0.0153 mm, and the dissolution time was about 7 minutes.

The fibrous chitosan of FIG. 2 had an average thickness of 0.0215 mm, and the dissolution time was about 9 minutes.

The fibrous chitosan of FIG. 3 had an average thickness of 0.03979 mm and a dissolution time of about 13 minutes.

The fibrous chitosan of FIG. 4 had an average thickness of 0.2697 mm and was found to be completely non-soluble within 1 hour.

The powdery chitosan of FIG. 5 was found to have an average particle size of 0.332386 mm, 0.195323 mm and 0.350297 mm, respectively.

The powdered chitosan of FIG. 6 was found to have an average particle size of 0.296869 mm, 0.390526 mm and 0.222697 mm, respectively.

Experimental Example 2: Fibrous Chitosan Deacetylation Solubility test

The solubility was measured using a fibrous yarn (prepared using only chitosan) having the same thickness (0.025 mm) except that each had a different degree of deacetylation. The solvent used was acidic water having a pH of 4 (1.2 wt% of fibrous chitosan, acidic water of 98.8 wt%) obtained by dissolving glycolic acid in water, and the results of the solubility test are shown in FIG.

As shown in FIG. 7, it was confirmed that the solubility was increased with increasing degree of deacetylation. Particularly, in the cases of 70% and 75% of deacetylation, it was confirmed that the solubility was so low that the undissolved solids were visually large enough to be difficult to apply to the skin. On the other hand, in the case of 80% to 90% And the solubility was high enough that it could be used as a cosmetic product. In the case of 90% or more, it was confirmed that the undissolved solid matter showed a high solubility such that it could not be visually recognized.

Experimental Example 3: Chitosan Fibrous Chitosan and Powder Dissolution time according to the content of chitosan and Obtained Test for measurement of physical properties of chitosan solution

Based on the above Experimental Examples 1 and 2, the chitosan powder and fibrous chitosan were prepared using chitosan having a degree of deacetylation of 95%, and the solubility and physical properties of the chitosan solution obtained after dissolution were measured. The results are shown in Table 1 1-1 to Table 1-6). In this case, the powdery chitosan had an average particle diameter of 0.298 mm, the fibrous chitosan had an average thickness of 0.025 mm, and the solvent used was acidic water having a pH of 4 adjusted with purified water or glycolic acid. The viscosity was measured at room temperature (25 캜). In Table 1, chitosan insolubility means insoluble in neutral water, soluble in acidic water (glycolic acid is used as AHA acid), powder means powder, and yarn means fibrous. In Table 1, chitosan water solubility means soluble in neutral water, powder means powder, and yarn means fibrous.

[Table 1-1]

[Table 1-2]

[Table 1-3]

[Table 1-4]

[Table 1-5]

[Table 1-6]

With respect to the dissolution time in the test results shown in Table 1, supposing that the cosmetics are used in a normal environment, it is considered to be caused by the measurement at a random temperature without controlling the temperature, The measurement error is not clarified. Although the dissolution time was somewhat irregular, the powdery chitosan and fibrous chitosan tended to increase with the increase of the total amount of the powder, but the dissolution time of the fibrous chitosan tended to increase more. This is considered to be due to the difference in the surface area depending on the shape.

As a result of the measurement errors, the pH of the fibrous chitosan decreased with increasing the amount of the powdery chitosan and the fibrous chitosan, , Respectively.

The viscosity of the powdery chitosan and the fibrous chitosan are increased with the increase of the amount of the powdery chitosan and the fibrous chitosan, The higher the viscosity of fibrous chitosan was, the higher the viscosity of fibrous chitosan was.

Experimental Example 4: Storage test of chitosan solution

The change in storage of the chitosan solution obtained by dissolving 1.2% by weight of chitosan having a deacetylation degree of 95% as used in the above Experiments in 98.8% of purified water was examined.

As shown in FIG. 8, when the chitosan solution was refrigerated (average refrigeration temperature: 4 to -5 ° C), no mold was formed even after 30 days of refrigerated storage. However, as shown in FIG. 9, Temperature: 25 캜). In case of storage, it was confirmed that the fungus was grown and grown at 10 days after storage at room temperature.

Experimental Example 5: Sensory test for preference by age

Chitosan solutions were prepared according to the concentrations of 0.5 wt% to 2.0 wt% (the remaining amount was the acidic water used in the above Experimental Examples), and adult women in their 20s to 50s were selected for each age group (i.e., 20s, 30s , 40, and 50), and a total of 120 panelists, each consisting of 30 individuals, were given each panel a total of 16 kinds of chitosan solutions for each concentration, and after using for 1 month, A preference test was conducted to check the chitosan solution of the most preferred concentration at the time of elapsed time, and the results are shown in Table 2 below. For the fairness of the experiment, the chitosan solutions were prepared in such a manner that the chitosan solutions at respective concentrations were incremented by 0.5 wt% to 0.1 wt% in the range of 0.5 wt% to 2.0 wt% for each concentration, and the prepared chitosan solutions had a high concentration (A to P) are sequentially assigned to the panel.

[Table 2]

As shown in Table 2, the chitosan concentration within the range of 0.9% to 1.3% was generally higher, especially within the range of 1.0% to 1.2%, and 1.1% and 1.2% . Among them, 1.2% concentration was the most preferred. Particularly, the concentration of 1.1 wt% was even higher in the 20s and 40s, but it was confirmed that 1.2 wt% was particularly high in the 40s and 50s .

Experimental Example 6: lifting Effect test

Based on the above experimental examples, fibrous chitosan having an average thickness of 0.025 mm was prepared using chitosan having a degree of deacetylation of 95%, and glycolic acid was dissolved in purified water to prepare acidic water having pH 4, A cosmetic composition was prepared by dissolving 1.2 wt% of the fibrous chitosan in 98.8 wt% and used in the lifting effect test.

The lifting effect test was performed as follows.

Testing period: Jan 08, 2015 - Jan 23, 2015

Test product: The cosmetic composition

Purpose: To evaluate the effect of the cosmetic composition of the present invention on once-weekly and twice-weekly use of eyebrow lifting, mouth lifting, face sagging, wrinkle, neck wrinkle, moisture content and exfoliation improvement

Test subjects: women aged 20 to 55 who meet the test subject selection and exclusion criteria

Number of subjects: 21

Test Subject Selection Criteria: Those who meet the following criteria

1) a woman of 20 to 55 years of age

2) Those who voluntarily signed the agreement after hearing enough explanation about the purpose and contents of the examination

3) Those who can follow up during the test period

4) Those who are not included in the exemption criteria

Eligibility Criteria for Eligibility: Eligibility for one of the following exclusions is not available.

1) If you do not want or do not fill out the agreement

2) If you have a psychiatric illness

3) In case of infectious skin disease

4) If you received immunosuppressant treatment within 3 months

5) If you have received steroid or light therapy within one month

6) If there is a lesion on the test site and measurement is difficult

7) If you have atopic skin

8) Cosmetics, medicines or allergic reactions to light exposure on a daily basis

9) Skin skeling or skin care within 3 months from the date of participating in the test

10) If you are pregnant or breastfeeding

11) In addition to the above, if it is judged that it is difficult to carry out the human body test at the judgment of the responsible researcher or the test person

Test method: We agreed to participate in this human body test, and we used the test product for 2 weeks for the subjects who satisfy the selection criteria and exclusion criteria. The test subjects used the test product at visit 1 and evaluated the test site before use and immediately after use. At the second visit, the same test site was evaluated 2 weeks after using the product, and the validity and likelihood questionnaire evaluation was completed after the test.

Evaluation items:

1) Mechanical evaluation

- Skin (eye tail, mouth, ball) lifting measurement (F-ray)

- Skin wrinkle measurement (Primos Pico)

- Measurement of skin neck wrinkles (Primos Pico)

- Measurement of skin moisture content (Corneometer)

- Skin keratin measurement (Visioscan VC98)

2) Global Assessment of Efficacy:

3) Safety evaluation: The researcher evaluates the results of the visual evaluation of the researchers and the results of the questionnaires of the subjects to be tested at each visit.

Other investigations (observation):

1) Demographic survey: Gender, date of birth and age before the test

2) Vital Signs Surveys: Before the start of human body test,

3) Examination of medical history: Examination of the main symptom, onset date, examination and treatment history before the start of human body test

4) Product preference survey: Survey of product preference through questionnaire

Visiting schedule: Two visits

1) Visit 1: Test subject agreement, test subject selection test, measurement before product use, measurement after product distribution

2) Visit 2: Adverse reaction confirmation, measurement after 2 weeks of product use, questionnaire evaluation

The primary efficacy variables are: skin tear-tail lifting, mouth lifting, ball lifting, wrinkle, neck wrinkle, water content,

Secondary Valuation Variables: Global Assessment of Efficacy Results

Safety evaluation: Evaluate the abnormal symptoms that appear after using the test product.

Evaluation area and measurement method: For the mechanical evaluation, the subject is stabilized for 30 minutes in a room with a temperature of 20 to 25 and a humidity of 40 to 60% under a constant temperature and humidity condition to adapt the skin surface temperature and humidity to the environment of the measurement space , And water intake was restricted during stabilization. For the objective measurement, one researcher measured the same area at each measurement.

Skin lifting measurement: The skin lifting measurement was performed by analyzing the images of eye tails, balls, and mouth with Image-pro plus using F-ray, which is a device that expresses the contours of the skin.

The eye - tail lifting was evaluated by measuring the angle with the line extending from the tip of the eye tip to the tip of the eye tail. The greater the angle between the two lines, the more noticeable is the lifting effect of the eye tail.

Facial (ball) lifting was based on the circle formed near the cheekbones, and a straight line was drawn from the center of the circle. It can be seen that the smaller the angle between the two straight lines is, the more the ball lifting effect is.

The lifting of the mouth was evaluated by measuring the angle between the tip of the tip of the mouth and the line drawn from the center of the circle by the circle formed near the cheekbone by drawing a straight line around the tip of the tip of the mouth. As the angle between the two lines increases, the effect of lifting the mouth is found.

Skin wrinkle wrinkle measurement: The skin wrinkle wrinkle was measured before and after the use of the test product by the 3D skin photographing device (Primos Pico) and after 2 weeks, the same area was photographed on the wrist portion and the skin wrinkling parameter Ra (average roughness : average roughness) were analyzed.

Skin neck wrinkle measurement: The skin neck wrinkle was measured before and after the use of the test product by the 3D skin photographing device (Primos Pico), and two weeks after the use, the neck area was photographed and the skin wrinkle parameter Ra : average roughness) were analyzed.

Measurement of skin moisture content: The skin moisture content was measured before use of the test product, immediately after use, and 2 weeks after using the Corneometer CM825 (Courage-Khazaka electronic GmbH, Germany). Measurements were made three times through a sensor with a corneometer probe on the skin, and the average value was used as the skin moisture content evaluation data. The corneometer can measure the moisture content by measuring the capacitance at the point where the probe touches. The unit is the unit constant, A.U, and the amount of skin moisturizing is proportional to the measured value.

Skin keratin measurement: The skin keratin measurement was measured before and after the test product was used and 2 weeks after using the Visioscan VC98 (Courage-Khazaka eletronic GmbH, Germany). The corneal epithelium was collected by using a special film (Corneofix), and images were taken with Visioscan VC98 and analyzed. The measured value DI (Desquamation Index) .

In this formula,

D is the epidermal decay index (%), A is the percentage of the area covered with keratinocytes, Tn is the percentage of keratinocytes associated with thickness, and n is the level of thickness (1-5).

Global Assessment of Efficacy: Very good (4), good (3) about the degree of eyebrow lifting, mouth lifting, facial deflection (ball lifting), wrinkle, neck wrinkle, ), Normal (2), poor (1), and very bad (0). The researchers determined the efficacy of the test product by obtaining the percentage of the test subjects for each answer.

Safety evaluation: The safety of the test product was determined by combining the adverse reactions identified in all the test subjects using the test product and all the adverse reactions reported during the test period, and used as the safety evaluation data of the product.

Product preference questionnaire evaluation: The subjects were asked to answer the questionnaire about the product feelings after using the test product. The evaluation items were examined for skin moisturizing, smoothness, softness, spreadability, degree of incense, overall feeling, and it was found to be very good (4), good (3), moderate (2), poor (1) The evaluation was made in five steps.

Assessment of adverse reactions: The abnormal skin symptoms that occurred during the use of the test product were surveyed during the test period to determine the occurrence and symptom level. If the subject felt any abnormal symptoms, they were instructed to report immediately to the examiners. The investigator informed the responsible investigator if an adverse reaction was reported, and the investigator determined the severity of the symptoms, the relationship with the test product, and decided whether to take appropriate measures and participate in the test.

Evaluation standard:

The primary efficacy parameters of the test product were skin moisture content, wrinkles (forehead, eyes, forehead), pores, brightness of the eyes, lifting, quickness of elasticity, resilience, .

Secondary efficacy parameters: The secondary efficacy parameters of the test product were evaluated based on the results of the efficacy evaluation questionnaire on the skin improvement effect after using the test product.

Statistical analysis: Statistical analysis program SPSS 19.0 was used to determine the significance of the measured values compared to the test product use. In the 95% confidence interval, the significance was confirmed when the significance probability was p <0.05, and the significance probability was rounded to the third decimal place. Continuous variables are summarized by mean and standard deviation, and categorical variables by frequency and percentage. Repeated measures ANOVA was used for the repeated measurement data more than 3 times, and it was postmarked by the Bonferroni method. A nonparametric method was used to compare the Friedman test with the Wilcoxon signed rank test, followed by post-test by adjusting the significance level with the Berserney method.

Experimental results are shown in Fig. 10 (eye lifting), Fig. 11 (mouth lifting), Fig. 12 (ball lifting), Fig. 13 (wrinkle wrinkle improvement), Fig. 14 (neck wrinkle improvement) (Skin exfoliation improvement).

As shown in Fig. 10, the eyeball lifting angle was measured two weeks before use in order to confirm the change of the eye-tail lifting value by using the test product. The taurine lifting angle measured 46.08 ± 10.42 before use, 46.78 ± 10.56 immediately after use, and 48.10 ± 10.33 after 2 weeks of use. Changes in skin lifting angle were statistically significant. The post hoc Bonferroni correction, a parametric method according to the normality test, showed that the angle of the eye tail lifting increased significantly (p <0.05) both immediately after use and after 2 weeks there was.

As shown in FIG. 11, the mouth lifting angle was measured two weeks before use to confirm the change of the mouth lifting value by using the test product. The mouth lifting angle measurements were 41.1 ± 17.18 before use, 41.70 ± 7.07 immediately after use, and 42.90 ± 7.17 after 2 weeks of use. Changes in skin lifting angle were statistically significant. As a result of the repeated measures ANOVA (post hoc Bonferroni correction), the lifting angle was significantly increased (p <0.05) both immediately after use and after 2 weeks .

As shown in FIG. 12, the ball lifting angle was measured before use and after 2 weeks of use in order to confirm the change of the ball lifting value by using the test product. The ball lifting angle measurements were 36.34 ± 7.40 before use, 35.74 ± 7.33 immediately after use, and 34.95 ± 7.34 after 2 weeks of use. Changes in skin lifting angle were statistically significant. As a result of the repeated measures ANOVA (post hoc Bonferroni correction), the ball lifting angle was significantly decreased (p <0.05) both immediately after use and after 2 weeks .

As shown in Fig. 13, the wrinkles of the wrists were measured 2 weeks before use to confirm changes in wrinkle wrinkles by using the test product. The measurement of wrinkles was 19.87 ± 2.96 before use, 18.76 ± 2.64 immediately after use, and 18.32 ± 2.86 after 2 weeks of use. The wrinkles of the skin were measured by statistical methods. The wrinkles were significantly reduced (p <0.025) immediately after use and after 2 weeks compared to before use, using the non-parametric Friedman test (post hoc Wilcoxon signed rank test with Bonferroni correction) I could confirm.

As shown in Fig. 14, neck wrinkles were measured two weeks before use to confirm changes in neck wrinkles by use of the test product. The neck wrinkles were 23.88 ± 2.75 before use, 22.44 ± 3.19 immediately after use, and 21.79 ± 2.88 after 2 weeks of use. The change of skin neck wrinkles was statistically significant. As a result of repeated measures ANOVA (post hoc Bonferroni correction), the neck wrinkle angle was significantly decreased (p <0.05) both immediately after use and after 2 weeks .

As shown in FIG. 15, the skin moisture content was measured two weeks before use to confirm the change in the water content by the use of the test product. Skin moisture content was 61.48 ± 9.33 before use, 82.79 ± 8.48 immediately after use, and 71.96 ± 7.56 after 2 weeks of use. Changes in skin moisture content were statistically analyzed to determine whether there was a significant difference. As a result of the repeated measures ANOVA (post hoc Bonferroni correction), the water content was significantly increased (p <0.05) immediately after use and after 2 weeks compared with before use.

In addition, as shown in FIG. 16, the skin angle mass was measured before use and two weeks after use in order to confirm changes in keratin values by use of the test product. Skin keratin measurements were 19.59 ± 2.59 before use, 13.60 ± 2.82 immediately after use, and 15.48 ± 2.61 after 2 weeks of use. The change of skin angle was examined statistically. The results of the Friedman test (post hoc Wilcoxon signed rank test with Bonferroni correction) showed that the skin keratin was decreased significantly (p <0.025) both immediately after use and after 2 weeks I could confirm.

The results of the questionnaire were as follows: Questionnaire value, standard deviation, and answer in the questionnaire survey on the degree of eyebrow lifting, mouth lifting, facial deflection (ball lifting), wrinkle, neck wrinkle, The percentages of the subjects were determined, and the results are shown in Table 3 below.

[Table 3]

As a result of the questionnaire evaluation, more than 90% of the subjects were rated more than normal for the degree of eye lifting, mouth lifting, facial deflection (ball lifting), wrinkles, neck wrinkles,

Product Preference Survey Results: After the product use survey, the preference of the test subjects to the skin moistness, smoothness, softness, spreadability, degree of fragrance, and overall feeling of the test product was examined. The results are shown by the mean standard deviation of the answers for each item, and the results are shown in Table 4 below.

[Table 4]

As a result of the survey, more than 85% of the test subjects were judged to be more than average in skin moistness, smoothness, softness, spreadability, degree of fragrance, and general feeling of use.

Test results: In this test, women who are 20 to 55 years old are allowed to use the test product once and for two weeks at the prescribed test site. After 2 weeks from the use of the product, the skin is lifted by eyebrow lifting, mouth lifting, Lifting, wrinkles, neck wrinkles, moisture content, and keratin were measured. After the completion of the test, the subjects' questionnaire evaluation was completed and the following conclusions were obtained.

1) All 21 subjects participated in this study were female, the mean age was 40.05 years, and all 21 subjects completed the test. The selected subjects had no specific skin symptoms and had no disease or drug use that could affect the test.

2) After the use of the test product, the measurement of the taurine lifting increased significantly (p <0.05) both immediately after use and after 2 weeks.

3) After the use of the test product, the measured value of the lifting was significantly increased (p <0.05) both immediately after use and after 2 weeks.

4) After measuring the ball lifting after using the test product, the angle measurement value decreased significantly (p <0.05) immediately after use and after 2 weeks.

5) The wrinkles of the wrinkles were measured after using the test product, and the wrinkles of the wrinkles were significantly decreased (p <0.025) immediately after use and after 2 weeks.

6) The neck wrinkles were measured after using the test product, and the neck wrinkles were significantly decreased (p <0.05) immediately after use and after 2 weeks.

7) The moisture content of the test product was significantly increased (p <0.05) both immediately after use and after 2 weeks.

8) As a result of measuring keratin quality after using the test product, each mass measurement value was significantly decreased (p <0.025) immediately after use and after 2 weeks.

9) As a result of the validation questionnaire survey on the test product, more than 90% of the subjects were evaluated to be more than average for eye lifting, mouth lifting, face lifting, wrinkle, neck wrinkle, moisture content, .

10) There were no reports of adverse reactions during the use of this test product by 21 subjects. No physical abnormalities such as erythema, rash, and pruritus were observed by physical examination by a dermatologist.

Therefore, the cosmetic composition according to the present invention can be used for a two-week use, which can improve immediate eye tread lifting, improve mouth lift, improve ball lifting, wrinkle wrinkle, neck wrinkle, It is a product that helps improve eye tuck lifting, improve lifting of mouth, improve face lift (ball lifting), wrinkle wrinkle, neck wrinkle, water content, and skin texture.

The present invention can be used in the field of business.

While the invention has been shown and described with reference to certain exemplary embodiments thereof, it will be understood by those skilled in the art that various changes and modifications may be made without departing from the spirit and scope of the invention as defined by the appended claims.

(Without reference numerals)

Claims

Dissolution within a range of 1 to 15 minutes in a solvent having a thickness within a range of 0.010 to 0.050 mm, a degree of deacetylation (DA: Degree of Deacetylation) within a range of 80 to 100% and a pH within a range of 3.5 to 7.5 1.7 to 4.0 wt% of a readily available fibrous chitosan that dissolves at a rate of from about 1 to about 10 wt%; And a solvent having a pH within a range of 3.5 to 7.5 as the balance, wherein the fibrous chitosan is contained in a state that the fibrous chitosan is not physically separated and dissolved in the solvent. A cosmetic composition for lifting.

The method according to claim 1,
Wherein the fibrous chitosan has a degree of deacetylation within a range of 90 to 100%.

delete

The method according to claim 1,
Wherein the solvent is water; (Vitamin C), ascorbyl phosphate, ascorbyl glucoside, magnesium ascorbyl phosphate, sodium ascorbyl phosphate, ascorbyl phosphate, ascorbyl palmitate, ascorbyl palmitate, ascorbyl palmitate, ascorbyl palmitate, Wherein the pH adjuster is dissolved in an acidic aqueous solution selected from the group consisting of palmitic acid, palmitic acid, palmitic acid, palmitic acid, palmitic acid, palmitic acid, palmitoyl palmitate, and palmitic acid.