WO2015169181A1

WO2015169181A1 - Electrostatic spinning collection apparatus and method, and electrostatic spinning device

Info

Publication number: WO2015169181A1
Application number: PCT/CN2015/078146
Authority: WO
Inventors: 弥胜利; 孙伟; 孔彬; 陈元筠
Original assignee: 清华大学深圳研究生院; 烟台上普瑞源生物科技有限公司
Priority date: 2014-05-04
Filing date: 2015-04-30
Publication date: 2015-11-12
Also published as: CN103981579A; CN103981579B

Abstract

Disclosed are an electrostatic spinning collection apparatus, and an electrostatic spinning device having same. The apparatus comprises a fibre orientation collector and a rotatable planar fibre receiver. The fibre orientation collector comprises a rotating shaft (5), two parallel dishes (7) installed on the rotating shaft (5), and a drive unit coupled to the rotating shaft, wherein a negative voltage is applied to the dishes, and a fibre which is formed by splitting a charged jet stream released from a positive voltage end is orientationally suspended between the two parallel dishes (7). The rotatable planar fibre receiver comprises a receiving disc (16) and a rotation drive mechanism and a reciprocation drive mechanism which are coupled to the receiving disc, wherein the reciprocation drive mechanism is used for bearing the receiving disc to extend into or withdraw from the region between the two parallel dishes; and the rotation drive mechanism is used for rotating the receiving disc to adjust an angle of a fibre collected on the disc surface of the receiving disc. Also disclosed is an electrostatic spinning collection method using the electrostatic spinning collection apparatus. The fibre which is electrostatically spun by means of the apparatus or device and the method has a good orientation degree and is suitable for constructing a highly ordered fiber texture structure.

Description

Electrospinning collecting device, method and electrospinning device

Technical field

The invention relates to the field of electrospinning, in particular to an electrospinning collecting device, an electrospinning collecting method and an electrospinning device.

Background technique

Formhals published the world's first patent on electrospinning in 1934, first proposed the concept of electrospinning. In the past ten years, with the rapid development of nanomaterial technology and interdisciplinary fields, electrospinning technology has received extensive attention from researchers.

In the traditional electrospinning, under the action of high voltage, the droplets suspended at the tip of the syringe overcome the surface tension of the syringe to form a charged jet. The flight path of the jet in the electric field is a three-dimensional "jitter of bending instability. Whip." After the solvent is volatilized or solidified, it is collected into the fiber collector in the form of a nonwoven fabric.

Traditional electrospinning technology electrospun fibers are disorganized and randomly distributed, mainly used for wound dressings, filter barriers, transparent reinforcing materials, etc., which greatly limits their application, especially in tissue engineering. Application because it is not suitable for building highly organized organizations.

Summary of the invention

The main object of the present invention is to overcome the deficiencies of the prior art and to provide an electrospinning electrospinning apparatus having a good degree of fiber orientation.

Another object is to provide an electrospinning method for electrospinning the degree of fiber orientation.

Still another object is to provide an electrospinning apparatus having the electrospinning collection device.

To achieve the above object, the present invention adopts the following technical solutions:

An electrospinning collection device comprising a fiber-oriented collector and a rotatable planar fiber collector, the fiber-oriented collector comprising a rotating shaft, two parallel disks mounted on the rotating shaft, and coupled to the rotating shaft a driving unit, the driving unit is configured to drive the rotating shaft to drive the disc to rotate, and a negative voltage is applied to the disc, and the fiber formed by the splitting of the charged jet released from the positive voltage end is suspended by the electric field force. Between parallel discs, the rotatable planar fiber retractor includes a take-up reel and a rotary drive mechanism coupled to the take-up reel and a reciprocating drive mechanism for carrying the retractable disc into or out Between two parallel disks, the rotary drive mechanism is configured to rotate the take-up disk to adjust the collection of fibers onto the disk surface of the take-up disk Angle.

In a further technical solution:

The fiber directional collector further includes a distance adjustment mechanism mounted on the rotating shaft for adjusting a distance between two parallel disks.

The distance adjusting mechanism includes a clamp that clamps the disc from both sides of the disc, the jig is sleeved on the rotating shaft, and the jig and the rotating shaft are threaded to be relatively movable in the axial direction. Way to connect.

The disc is circular, and the distance between the two parallel discs is adjustable from 38 to 90 mm.

The two ends of the rotating shaft are fixed on a pair of brackets, and the rotating shaft is electrically connected to a negative voltage through one of the brackets, the disc is electrically connected to the rotating shaft, and the driving unit is installed on the outer side of the other bracket and passes through A motor in which the coupling is coupled to the rotating shaft.

The shuttle drive mechanism includes a horizontal drive source and a wedge frame connecting the horizontal power source, the rotary drive mechanism including a rotary drive source and a vertical shaft connecting the rotary drive source, the vertical shaft being rotatably mounted on the vertical shaft A front end of the wedge frame, the take-up disk being mounted at a top end of the vertical shaft.

The take-up disk is circular, and the disk surface is parallel to the rotating shaft. Preferably, the charging disk is rotatable at an angle of 0 to 180 degrees.

An electrospinning collection method using the electrospinning collection device comprises the following steps:

While the rotating shaft drives the rotation of the two parallel discs, the reciprocating driving mechanism drives the collecting disc at the rotation angle of the first disc surface to reciprocate uniformly between the two parallel discs, and collects the oriented fibers suspended between the two parallel discs.

Preferably, the uniform speed of movement ranges from 0 to 10 mm/s.

Further, the method further includes:

Rotating drive mechanism rotates the take-up disk to a second disk surface rotation angle;

While the rotating shaft drives the rotation of the two parallel discs, the reciprocating drive mechanism drives the position

The take-up disk of the second disk surface rotation angle reciprocates smoothly between the two parallel disks,

The oriented fibers suspended between the two parallel discs were collected.

An electrospinning apparatus comprising the electrospinning collection device, a syringe connected to a positive voltage, and a syringe pump in communication with the syringe, the syringe being above the two parallel discs of the electrospinning collection device, A charged jet for ejecting an electrospinning solution in the direction of two parallel discs.

Advantageous technical effects of the present invention:

The present invention utilizes a parallel dish by providing a collecting device having two parallel disks and connecting a negative voltage The resulting electric field causes the fibers to be oriented between two parallel discs with a high degree of fiber orientation; the oriented fibers are charged as needed by a take-up disc of a rotatable planar fiber retractor, and the take-up disc is rotatable to adjust the oriented fibers at the pick-up disc The upper direction of collection allows for the controlled orientation of the electrospun fiber product. By controlling the angle of rotation of the disk, it is possible to control the angle between the multilayer fiber product layer and the layer fibers. The invention is particularly suitable for use in tissue engineering to construct highly ordered tissues, for example, the use of the present invention allows for the fabrication of highly oriented electrospun multilayer oriented nanofiber scaffolds.

DRAWINGS

1 is a schematic structural view of a fiber orientation device according to an embodiment of the present invention;

2 is a schematic structural view of a fiber collecting device in an embodiment of the present invention;

3 is a schematic structural view of an electrospinning device according to an embodiment of the present invention;

Figure 4 is a scanning electron micrograph of a uniaxially oriented polyvinyl alcohol fiber scaffold electrospun using an embodiment of the present invention;

Figure 5 is a scanning electron micrograph of a biaxially oriented polyvinyl alcohol fiber scaffold electrospun using an embodiment of the present invention.

The markings in the figure are as follows: 1-base; 2-bracket; 3-bolt set; 4-high voltage power supply; 5-axis; 6-clamp; 7-disc; 8-injector tip; 9-fiber; 10-bearing; - motor box; 12-power supply; 13-wedge frame; 14-power box; 15-power supply; 16-receiving disc; 17-vertical shaft.

detailed description

Embodiments of the present invention will be described in detail below with reference to the accompanying drawings. It is to be understood that the following description is only illustrative, and is not intended to limit the scope of the invention.

Referring to Figures 1-3, in accordance with an embodiment of the present invention, an electrospinning collection apparatus includes a fiber-oriented collector and a rotatable planar fiber collector, the fiber-oriented collector including a rotating shaft 5, two parallel mounted on the rotating shaft 5 The disc 7 and a driving unit coupled with the rotating shaft 5, the driving unit is for driving the rotating shaft 5 to drive the rotating of the disc 7, the negative voltage is applied to the disc 7, and the fiber 9 formed by the splitting of the charged jet released from the positive voltage end is affected by the electric field force. The directional suspension is between the two parallel discs 7. The rotatable planar fiber retractor comprises a take-up disc 16 and a rotary drive mechanism coupled to the take-up reel 16 and a reciprocating drive mechanism for carrying the retractable disc 16 into or Between the two parallel discs 7, the rotary drive mechanism is used to rotate the take-up disc 16 to adjust the angle at which the fibers are collected onto the disc surface of the take-up disc 16. The oriented fibers collected between the two parallel discs 7 in the fiber-oriented collector can be collected onto the collecting tray 16 of the rotatable planar fiber retractor by physical dragging during rotation with the parallel disc 7. . Fiber oriented collector and rotatable planar fiber collector The combination can realize the preparation of a nanofiber support with a controlled thickness and a thickness of up to millimeters.

As shown in Figure 1, in a specific embodiment, the fiber-oriented collector comprises two parallel ultra-thin circular discs, an externally threaded shaft 5 for mounting a circular disc, and a fixed circular disc with a fitting Threaded clamp 6 and bracket 2. The rotating shaft 5 is mounted on the bracket 2 via a bearing 10, and the bracket 2 is fixed to the base 1, and is connected to the base 1 through the bolt group 3. The shaft 5 and the clamp 6 are screwed together. Two parallel discs 7 are fixed to the rotary shaft 5 by means of a clamp 6. By the screwing engagement, the relative position between the jig 6 and the rotating shaft 5 can be adjusted, and the distance between the two parallel discs 7 can be adjusted. A preferred adjustment range is 38 to 90 mm. The driving unit comprises a motor box 11 disposed outside the bracket 2. The motor in the motor box 11 is powered by the power source 12. The motor drives the shaft 5 to rotate through the coupling, thereby driving the two parallel discs 7 to rotate at a certain speed. The rotating shaft 5 is connected to the negative high voltage pole of the high voltage power source 4 through the bracket 2 to provide a negative high voltage for the two parallel disks 7.

As shown in FIG. 2, the rotatable planar fiber retractor can include a power mechanism and a circular take-up reel 16. The power mechanism includes a rotary drive mechanism and a reciprocating drive mechanism. The reciprocating drive mechanism can drive the circular take-up disk 16 to reciprocate at a constant speed, and the preferred speed range is 0 to 10 mm/s. During the round-trip motion, the circular take-up disk 16 can enter or exit the space between the two parallel disks 7, and the circular take-up disk 16 can be set to run at a maximum distance of exactly between the two disks 7. The disk surface of the take-up disk 16 is parallel to the rotating shaft 5, and the rotary drive mechanism can drive the circular take-up disk 16 to rotate, thereby adjusting the pickup angle of the fiber on the take-up disk 16. The take-up disk 16 can be rotated from 0 to 180 each time as needed.

Preferably, the rotatable planar fiber retractor includes a power box 14 and a wedge frame 13 that is powered by a power source 15 that provides a reciprocating driving force to the wedge frame 13. The wedge frame 13 can be driven to reciprocate at a constant speed, thereby driving the circular take-up disk 16 to reciprocate at a constant speed to collect the oriented fibers suspended between the two parallel plates 7. By rotating the vertical shaft 17 mounted on the wedge frame 13, the rotary drive mechanism in the power box 14 can control the rotation of the circular take-up disk by 0 to 180 degrees in a timed manner, thereby collecting the directional fiber holders which form 0-180 degrees of interlaced fibers per layer. . The round-trip distance of the wedge frame 13 can be appropriately controlled, and the timing rotation time of the circular take-up disk 16 can also be appropriately controlled as needed.

Figure 3 illustrates the mating relationship between the fiber oriented collector and the rotatable planar fiber picker. In operation, the circular take-up disc 16 of the rotatable planar fiber retractor travels back and forth between the two parallel discs 7 in the radial direction of the parallel disc 7, collecting the oriented fibers formed between the two parallel discs 7.

An electrospinning method can be implemented by using an electrospinning apparatus having the above collecting device, and the specific process is as follows:

The syringe located above the two parallel discs 7 is connected to the positive high pressure, and the rotating shaft 5 is connected to the negative high pressure, so that the two parallel discs 7 are connected with a negative high pressure. The droplets suspended by the needle tip 8 of the syringe overcome the surface tension under the action of electrostatic force to form a charged jet, which is formed by the electric field force between the two parallel discs 7 and is formed by the oriented fibers suspended between the two parallel discs 7, and then reciprocated. The circular collecting disk 16 is collected. When the circular receiving disk 16 is rotated by 0 to 180 degrees, the direction of the collected oriented fibers is also rotated by 0 to 180 degrees, thereby achieving an angle of 0 to 180 degrees between each layer of fibers. Preparation of oriented fiber scaffolds.

In the above process, the electrospinning solution can overcome the surface tension of itself to form a charged jet. Under the action of the electric field force between the two parallel and coaxial rotating discs 7, the solvent is volatilized and the fibers are suspended in parallel in two parallels. Between the discs 7, the degree of orientation is high. The circular take-up disc 16 in the rotatable planar fiber retractor can be rotated from 0 to 180 at a time to collect the oriented fiber support with an angle between 0 and 180 between each layer of fibers. The thickness of each layer of oriented fibers can be controlled by controlling the interval at which the extractor takes the oriented fibers from the two parallel discs 7.

According to a specific embodiment, the relative position between the syringe needle and the two parallel discs 7 can be adjusted so that the distance between the needle tip and the disc 7 is satisfactory; the flow rate of the electrospinning solution can be adjusted by the syringe pump; the syringe can be adjusted to be connected to the syringe. The positive high pressure of the needle and the negative high pressure connected to the rotating shaft 5; the rotating speed of the rotating shaft 5 of the parallel disc 7 collector can be adjusted, and the reciprocating speed and the rotation period of the circular take-up disc 16 in the rotatable plane fiber picker can be adjusted to control The distribution of different layers of fibers, the staggered angles of the different layers, and the like, which are collected by the disk 16, are collected.

Example 1:

The uniaxial PVA fiber scaffold was electrospun using the apparatus and method described above. The PVA concentration is 10%, the set solution flow rate is 0.3ml/h, the voltage is 15KV, the distance between the needle tip of the syringe and the parallel thin disc is 175mm, the rotational speed of the thin disc is 500r/min, and the distance between the parallel thin discs is 38mm. The electrospinning ambient temperature is 37. The rotatable planar fiber retractor is fixed in a position such that the circular take-up disc in the rotatable planar fiber retractor is positioned between the two thin discs, keeping the circular take-up disc and collecting the oriented fibers in one direction. The electrospinning time is 3h. The resulting uniaxially oriented fibers are shown in Figure 4.

Example 2:

The biaxial PVA fiber scaffold was electrospun using the apparatus and method described above. The PVA concentration is 10%, the set solution flow rate is 0.5ml/h, the voltage is 12KV, the distance between the needle tip of the syringe and the parallel thin disc is 175mm, the speed of the thin disc is 800r/min, and the distance between the parallel discs is 43mm. The circular take-up disk in the rotatable planar fiber retractor has a moving speed of 5 mm/s, a rotation period of 10 min, a rotation angle of 90 degrees, a rotational speed of 10 mm/s, and an electrospinning ambient temperature of 37. The electrospinning time is 2h. The resulting biaxially oriented fibers are shown in Figure 5.

The nanofiber scaffolds collected by the device can be micro-processed, such as mechanical or laser perforation, to increase the porosity of the scaffold, and facilitate the penetration of cells and cell secretory components in the scaffold, thereby facilitating the scaffolding. Application in tissue engineering.

The above is a further detailed description of the present invention in connection with the specific preferred embodiments, and the specific embodiments of the present invention are not limited to the description. It will be apparent to those skilled in the art that the present invention may be made without departing from the spirit and scope of the invention.

Claims

An electrospinning collection device comprising a fiber-oriented collector - and a rotatable planar fiber collector, the fiber-oriented collector comprising a rotating shaft, two parallel disks mounted on the rotating shaft, and a driving unit coupled to the rotating shaft, the driving unit is configured to drive the rotating shaft to drive the rotating of the disc, a negative voltage is applied to the disc, and a fiber formed by splitting the charged jet released from the positive voltage end is caused by an electric field force Oriented to hang between two parallel discs, the rotatable planar fiber retractor comprising a take-up disc and a rotary drive mechanism coupled to the take-up reel and a reciprocating drive mechanism for carrying the charge The disc extends into or out of the two parallel discs, and the rotary drive mechanism rotates the take-up disc to adjust the angle at which the fibers are collected onto the disc surface of the take-up disc.
The electrospinning collection apparatus according to claim 1, wherein said fiber-oriented collector further comprises a distance adjusting mechanism mounted on said rotating shaft for adjusting a distance between two parallel discs.
The electrospinning collecting apparatus according to claim 2, wherein said distance adjusting mechanism comprises a jig that clamps said disc from both sides of said disc, said jig being threaded over said rotating shaft, said The clamp is coupled to the rotating shaft by threads to be relatively movable in the axial direction.
The electrospinning collection device according to claim 2, wherein said dish is circular, and the distance between the two parallel plates is adjustable from 38 to 90 mm.
The electrospinning collection device according to any one of claims 1 to 4, wherein both ends of the rotating shaft are fixed to a pair of brackets, and the rotating shaft is electrically connected to a negative voltage through one of the brackets, The disk is electrically connected to the rotating shaft, and the driving unit is a motor mounted on the outer side of the other of the brackets and connected to the rotating shaft through a coupling.
The electrospinning collection device according to any one of claims 1 to 4, wherein the reciprocating drive mechanism comprises a horizontal drive source and a wedge frame connecting the horizontal power source, the rotary drive mechanism including a rotary drive And a vertical shaft connecting the rotary drive source, the vertical shaft is rotatably mounted on a front end of the wedge frame, and the take-up disk is mounted on a top end of the vertical shaft.
The electrospinning collection device according to any one of claims 1 to 4, wherein the take-up disk is circular, the disk surface is parallel to the rotating shaft, and preferably, the take-up disk is rotatable at an angle of 0. ~180 degrees.
Static electricity using the electrospinning collecting device according to any one of claims 1 to 7. The spinning collection method is characterized in that it comprises the following steps:

While the rotating shaft drives the rotation of the two parallel discs, the reciprocating driving mechanism drives the collecting disc at the rotation angle of the first disc surface to reciprocate uniformly between the two parallel discs, and collects the oriented fibers suspended between the two parallel discs, preferably, The uniform speed of motion is in the range of 0 to 10 mm/s.
The method of collecting electrospinning according to claim 8, further comprising:

Rotating drive mechanism rotates the take-up disk to a second disk surface rotation angle;

While the rotating shaft drives the rotation of the two parallel discs, the reciprocating driving mechanism drives the collecting disc at the rotation angle of the second disc surface to reciprocate uniformly between the two parallel discs, and collects and hangs on the two parallel discs. Aligned fibers.
An electrospinning apparatus comprising the electrospinning collection device according to any one of claims 1 to 7, a syringe connected to a positive voltage, and a syringe pump in communication with the syringe, the syringe being in a chamber Above the two parallel discs of the electrospinning device, a charged jet for ejecting the electrospinning solution in the direction of the two parallel discs.