WO2015145527A1 - 光照射によって翼形類およびフジツボ類を殺す方法 - Google Patents
光照射によって翼形類およびフジツボ類を殺す方法 Download PDFInfo
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- WO2015145527A1 WO2015145527A1 PCT/JP2014/058010 JP2014058010W WO2015145527A1 WO 2015145527 A1 WO2015145527 A1 WO 2015145527A1 JP 2014058010 W JP2014058010 W JP 2014058010W WO 2015145527 A1 WO2015145527 A1 WO 2015145527A1
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Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01M—CATCHING, TRAPPING OR SCARING OF ANIMALS; APPARATUS FOR THE DESTRUCTION OF NOXIOUS ANIMALS OR NOXIOUS PLANTS
- A01M29/00—Scaring or repelling devices, e.g. bird-scaring apparatus
- A01M29/06—Scaring or repelling devices, e.g. bird-scaring apparatus using visual means, e.g. scarecrows, moving elements, specific shapes, patterns or the like
- A01M29/10—Scaring or repelling devices, e.g. bird-scaring apparatus using visual means, e.g. scarecrows, moving elements, specific shapes, patterns or the like using light sources, e.g. lasers or flashing lights
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F1/00—Treatment of water, waste water, or sewage
- C02F1/30—Treatment of water, waste water, or sewage by irradiation
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/32—Biological treatment of water, waste water, or sewage characterised by the animals or plants used, e.g. algae
- C02F3/327—Biological treatment of water, waste water, or sewage characterised by the animals or plants used, e.g. algae characterised by animals and plants
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- E—FIXED CONSTRUCTIONS
- E02—HYDRAULIC ENGINEERING; FOUNDATIONS; SOIL SHIFTING
- E02B—HYDRAULIC ENGINEERING
- E02B1/00—Equipment or apparatus for, or methods of, general hydraulic engineering, e.g. protection of constructions against ice-strains
- E02B1/006—Arresting, diverting or chasing away fish in water-courses or water intake ducts, seas or lakes, e.g. fish barrages, deterrent devices ; Devices for cleaning fish barriers
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- E—FIXED CONSTRUCTIONS
- E04—BUILDING
- E04B—GENERAL BUILDING CONSTRUCTIONS; WALLS, e.g. PARTITIONS; ROOFS; FLOORS; CEILINGS; INSULATION OR OTHER PROTECTION OF BUILDINGS
- E04B1/00—Constructions in general; Structures which are not restricted either to walls, e.g. partitions, or floors or ceilings or roofs
- E04B1/62—Insulation or other protection; Elements or use of specified material therefor
- E04B1/72—Pest control
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2103/00—Nature of the water, waste water, sewage or sludge to be treated
- C02F2103/08—Seawater, e.g. for desalination
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2303/00—Specific treatment goals
- C02F2303/20—Prevention of biofouling
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W10/00—Technologies for wastewater treatment
- Y02W10/10—Biological treatment of water, waste water, or sewage
Definitions
- the present invention relates to a method for killing attached larvae of attached organisms.
- An object of the present invention is to provide a method for killing the attached stage larvae of attached organisms.
- the present inventor has found that when the LED light having a wavelength of 409 to 412 nm is irradiated, the adhesion stage larvae tend to stop activities such as swimming and eventually die, and have reached the present invention.
- One embodiment of the present invention is a method of killing an attached stage larva of an attached organism in water, the method comprising irradiating the attached stage larva with light having a wavelength of 409 to 412 nm. It is preferable that the light includes a part of wavelengths of 400 to 440 nm. The light preferably has a peak in a wavelength range of 409 to 412 nm. The light preferably includes a wavelength of 400 to 420 nm. The light preferably has a wavelength of 409 to 412 nm and an irradiation intensity with respect to the adhesion stage larvae of 146.4463 ⁇ Wcm ⁇ 2 nm ⁇ 1 or more. The light is preferably irradiated for 3 hours or more.
- the light may not be laser light.
- the light may be LED light.
- the water may be seawater.
- the adhesion stage larvae may be a mussel pediberger larvae, a planta grade larvae, or a red barnacle cypris larvae.
- the method for killing the attached stage larvae of attached organisms in water according to the present invention includes a step of irradiating the attached stage larvae with light having a wavelength of 409 to 412 nm. This can kill the adherent larvae.
- the target attached organisms are floating in the sea during the early larvae, and when they become attached larvae, they attach to appropriate attachments and transform into adults, such as mussels and barnacles. it can. Therefore, killing the attached larvae of attached organisms in water is preferably carried out in seawater, but is not limited to seawater, and is carried out in other concentrations of saltwater, such as fresh water and seawater mixed water. It may be performed in fresh water.
- the mussels are generic names for the clams of the mussel family (Mytilidae). ), Mytilinae, such as mussels, cypresses, and mussels.
- barnacles are a general term for those classified into Crustacea, Cirripadia, Thoracica, for example, vertebrate barnacles, American barnacles, red barnacles, sacred barnacles, giant red barnacles, These include those belonging to the Baranomorpha, including the Sarasah, Awaji, Aoshiro, and European barnacles.
- the generation stage to be irradiated with light is preferably an adhesion stage larva.
- the adhesion stage larvae can be prevented from adhering to the deposits.
- the attachment stage larvae correspond to pediberger larvae and planty grades, and in the case of barnacles, they correspond to cypris larvae.
- the deposits to which the attachment stage larvae adhere are not particularly limited, and examples thereof include a seawater intake facility or a seawater discharge facility, a coastal aquaculture facility, or a fishery facility of a power plant.
- the wavelength of light applied to the attachment stage larvae is light including a wavelength of 409 to 412 nm.
- the light preferably includes a part of the wavelength range of 400 to 440 nm (here, “part” does not include “all”), and preferably covers the entire wavelength range of 440 to 420 nm. It is preferable to include ultraviolet rays (wavelengths smaller than 400 nm), visible light (400 to 830 nm), and infrared rays (wavelengths larger than 830 nm). Since the wavelength in the range of 400 nm to 420 nm has higher permeability in seawater than ultraviolet light, the present invention can exert a light effect over a wider range than the method using light containing only ultraviolet light. Further, as shown in the examples, it is preferable that the light has a peak in the wavelength range of 409 to 412 nm. This light may not be laser light.
- the irradiation intensity and irradiation time of the light emitted to the attachment stage larvae are not particularly limited, and can be determined appropriately and easily by those skilled in the art depending on the irradiation environment (for example, water quality, water depth, transparency, etc.).
- the wavelength spectral irradiance at the position of the adhesion stage larvae is preferably 89.6946 ⁇ Wcm ⁇ 2 nm ⁇ 1 or more in part or all of the wavelength range of 409 to 412 nm, and is 12.1344 ⁇ Wcm ⁇ 2 nm ⁇ 1 or more is more preferable, and 146.44633 ⁇ Wcm ⁇ 2 nm ⁇ 1 or more is most preferable.
- the irradiation time is preferably 3 hours or more, more preferably 6 hours or more, further preferably 9 hours or more, and most preferably 12 hours or more.
- the irradiation time is preferably 15 hours or more, more preferably 1 day or more, further preferably 2 days or more, and most preferably 1 week or more.
- irradiation may be continuous or intermittent, in the case of being intermittent, it is preferable to set the total light irradiation time as described above.
- the irradiation method is not particularly limited.
- an LED irradiation device a mercury lamp, a fluorescent tube, or the like can be used as the light irradiation device, but an LED irradiation device is preferably used.
- a box-type device with a lid capable of shielding external light was prepared.
- This box-type device has an inner dimension of (length) 17.5 cm ⁇ (width) 17.5 cm ⁇ (depth) 10.0 cm, and the inside of the box is made of black PVC.
- an LED panel was mounted on the lid of the apparatus, and a stage was provided at a position where the distance from the LED panel inside the apparatus was 5 cm.
- a petri dish containing larvae was placed on this stage, and the larvae were irradiated with LED light vertically from above.
- an apparatus without an LED panel was used for the dark condition experiment.
- an LED light emitting element having a peak wavelength of 370 to 380 nm is mounted (manufactured by CCS, LED panel type: ISL-150X150UU375TPNL), and an LED light emitting element having a peak wavelength of 409 to 412 nm is mounted.
- LED CSC Co., Ltd., LED panel model: ISL-150X150-VV-TPNL
- LED LED light emitting element with a peak wavelength of 515 to 535 nm
- Table 1 shows the wavelength characteristics of each LED light.
- ADVANTEC membrane filter
- the petri dish was taken out from the apparatus, and the larvae were observed using a stereomicroscope. The larvae were observed for 10 minutes, and during this period, the individual who had only moved the first tactile sensation and the limbs slightly while falling to the bottom of the petri dish was counted as “weakened individuals”. Individuals who have no movement in the body and do not move the first tactile sensation or thorax, or whose inner shell is exposed outside the shell (back), Counted as “dead individuals” and removed from petri dish.
- Table 2 summarizes the experimental conditions and results.
- the irradiation illuminance described in “Irradiation conditions” in Table 2 is a value measured at a position 5 cm away from the LED panel.
- the temperature of the seawater under experiment is 26 degrees C or less, the influence by temperature can be excluded.
- the red cucumber larvae do not weaken the larvae even after 12 hours of irradiation when irradiated with LED light having a peak wavelength of 370 to 380 nm (15 W / m 2 ) or 515 to 535 nm (50 W / m 2 ). As in the dark condition, it was confirmed that they were actively swimming.
- the mussel pediberger larvae closed after 9 hours when irradiated with LED light having a peak wavelength of 370 to 380 nm (15 W / m 2 ), but did not die after 12 hours, but 515 to 535 nm (50 W / m 2). In the case of irradiation with the LED light), it was confirmed that the shell does not close even after 12 hours and actively swims and drowns as in the dark condition.
- the present invention has made it possible to provide a method for killing attached larvae of attached organisms in water.
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- General Engineering & Computer Science (AREA)
- Hydrology & Water Resources (AREA)
- Organic Chemistry (AREA)
- Chemical & Material Sciences (AREA)
- Water Supply & Treatment (AREA)
- Environmental & Geological Engineering (AREA)
- Pest Control & Pesticides (AREA)
- Physics & Mathematics (AREA)
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- Civil Engineering (AREA)
- Architecture (AREA)
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Abstract
Description
外部の光を遮光可能な、蓋付の箱型装置を用意した。この箱型装置は、内寸が(長さ)17.5cm×(幅)17.5cm×(深さ)10.0cmであり、箱の内側は黒色の塩ビ製である。明条件の実験については、この装置の蓋部にLEDパネルを装着し、装置内部のLEDパネルからの距離が5cmとなる位置にステージを設けた。試験の際は、幼生の入ったシャーレをこのステージ上に載置し、幼生に対して上方から垂直にLED光を照射した。暗条件の実験については、LEDパネルを装着していない装置を用いた。
==アカフジツボのキプリス幼生の選別==
付着生物であるアカフジツボの付着期幼生であるキプリス幼生に対して、放射照度100W/m2のプロジェクションライト光を実験直前まで1時間以上照射し、光源に近づくような行動をとり、かつ、活発に遊泳行動をとる個体を以下の実験に使用した。
==アカフジツボのキプリス幼生へのLED光の照射==
目合い3μmのメンブレンフィルター(ADVANTEC)でろ過した約22℃の海水20mlの入ったガラスシャーレ(水深1cm)にキプリス幼生をいれ、装置内部のステージ上に載置し、装置の蓋をして、LED光の照射を行った。
==ムラサキイガイのペディベリジャー幼生およびプランティグレイド幼生に対するLED光の照射==
ムラサキイガイのペディベリジャー幼生とプランティグレイド幼生は、10分間の観察中に遊泳や匍匐をすることなく、面盤や足を殻内に収納している閉殻状態の個体を「閉殻個体」として計数し、鰓運動のない状態の個体を「死んだ個体」として計数したこと以外は、アカフジツボのキプリス幼生と同様に実験を行った。表3および表4に、実験条件と結果をまとめた。表3および表4の「照射条件」に記載の照射照度は、LEDパネルから5cm離れた位置で測定した値である。
Claims (10)
- 付着生物の付着期幼生を水中で殺す方法であって、
前記付着期幼生に対し、409~412nmの波長を含む光を照射する工程を含む方法。 - 前記光が、400~440nmの波長域のうち一部の波長を含む、請求項1に記載の方法。
- 前記光が409~412nmの波長域においてピークを有することを特徴とする、請求項1または2に記載の方法。
- 前記光が400~420nmの波長を含むことを特徴とする、請求項1~3のいずれか1項に記載の方法。
- 前記光は、409~412nmの波長域で、前記付着期幼生に対する照射強度が146.4643μWcm-2nm-1以上の波長を有することを特徴とする、請求項1~4のいずれか1項に記載の方法。
- 前記光は、3時間以上照射することを特徴とする、請求項1~5のいずれか1項に記載の方法。
- 前記光がレーザー光でないことを特徴とする、請求項1~6のいずれか1項に記載の方法。
- 前記光がLED光であることを特徴とする、請求項1~7のいずれか1項に記載の方法。
- 前記水が海水であることを特徴とする、請求項1~8のいずれか1項に記載の方法。
- 前記付着期幼生が、ムラサキイガイのペディベリジャー幼生若しくはプランティグレイド幼生またはアカフジツボのキプリス幼生であることを特徴とする、請求項1~9のいずれか1項に記載の方法。
Priority Applications (4)
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JP2015559346A JP6215967B2 (ja) | 2014-03-24 | 2014-03-24 | 光照射によって翼形類およびフジツボ類を殺す方法 |
EP14886734.4A EP3124441B1 (en) | 2014-03-24 | 2014-03-24 | Method for killing pteriomorphia and barnacles using light irradiation |
US15/128,213 US11134669B2 (en) | 2014-03-24 | 2014-03-24 | Method for killing Pteriomorphia and barnacles using light irradiation |
PCT/JP2014/058010 WO2015145527A1 (ja) | 2014-03-24 | 2014-03-24 | 光照射によって翼形類およびフジツボ類を殺す方法 |
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PCT/JP2014/058010 WO2015145527A1 (ja) | 2014-03-24 | 2014-03-24 | 光照射によって翼形類およびフジツボ類を殺す方法 |
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EP (1) | EP3124441B1 (ja) |
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Cited By (5)
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EP3180978A4 (en) * | 2014-12-08 | 2017-11-01 | The Chugoku Electric Power Co., Inc. | Method for restraining adhesion of barnacles |
JP2017228889A (ja) * | 2016-06-21 | 2017-12-28 | 株式会社島津製作所 | 水中通信装置及び水中照射装置 |
US11134669B2 (en) | 2014-03-24 | 2021-10-05 | The Chugoku Electric Power Co., Inc. | Method for killing Pteriomorphia and barnacles using light irradiation |
US11134671B2 (en) | 2015-03-27 | 2021-10-05 | The Chugoku Electric Power Co., Inc. | Method for preventing settlement of sessile organisms |
US11140893B2 (en) | 2014-03-24 | 2021-10-12 | The Chugoku Electric Power Co., Inc. | Method for stopping swimming or crawling of adhesion-stage larvae |
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US11832606B1 (en) * | 2019-02-20 | 2023-12-05 | Scanlogx, Inc. | Organism eradication system and method of use |
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US11134669B2 (en) | 2014-03-24 | 2021-10-05 | The Chugoku Electric Power Co., Inc. | Method for killing Pteriomorphia and barnacles using light irradiation |
US11140893B2 (en) | 2014-03-24 | 2021-10-12 | The Chugoku Electric Power Co., Inc. | Method for stopping swimming or crawling of adhesion-stage larvae |
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US11134670B2 (en) | 2014-12-08 | 2021-10-05 | The Chugoku Electric Power Co., Inc. | Methods of suppressing settlement of barnacles |
US11134671B2 (en) | 2015-03-27 | 2021-10-05 | The Chugoku Electric Power Co., Inc. | Method for preventing settlement of sessile organisms |
JP2017228889A (ja) * | 2016-06-21 | 2017-12-28 | 株式会社島津製作所 | 水中通信装置及び水中照射装置 |
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EP3124441A1 (en) | 2017-02-01 |
EP3124441B1 (en) | 2020-04-22 |
EP3124441A4 (en) | 2017-03-22 |
JP6215967B2 (ja) | 2017-10-18 |
US11134669B2 (en) | 2021-10-05 |
JPWO2015145527A1 (ja) | 2017-04-13 |
US20170172135A1 (en) | 2017-06-22 |
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