WO2015132898A1 - Procédé de dispersion de particules magnétiques et dispositif de dispersion de particules magnétiques - Google Patents
Procédé de dispersion de particules magnétiques et dispositif de dispersion de particules magnétiques Download PDFInfo
- Publication number
- WO2015132898A1 WO2015132898A1 PCT/JP2014/055588 JP2014055588W WO2015132898A1 WO 2015132898 A1 WO2015132898 A1 WO 2015132898A1 JP 2014055588 W JP2014055588 W JP 2014055588W WO 2015132898 A1 WO2015132898 A1 WO 2015132898A1
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- magnetic particles
- magnetic
- partition member
- agglomerated
- container
- Prior art date
Links
- 239000006249 magnetic particle Substances 0.000 title claims abstract description 170
- 238000000034 method Methods 0.000 title claims description 39
- 238000005192 partition Methods 0.000 claims description 84
- 239000006185 dispersion Substances 0.000 claims description 38
- 239000000463 material Substances 0.000 claims description 18
- 229910052751 metal Inorganic materials 0.000 claims description 7
- 239000002184 metal Substances 0.000 claims description 7
- 239000000057 synthetic resin Substances 0.000 claims description 4
- 229920003002 synthetic resin Polymers 0.000 claims description 4
- 239000000919 ceramic Substances 0.000 claims description 3
- 239000011521 glass Substances 0.000 claims description 3
- 229910052710 silicon Inorganic materials 0.000 claims description 3
- 239000010703 silicon Substances 0.000 claims description 3
- 238000000926 separation method Methods 0.000 abstract 4
- 238000005299 abrasion Methods 0.000 abstract 1
- 230000000717 retained effect Effects 0.000 abstract 1
- 238000007400 DNA extraction Methods 0.000 description 33
- 102000053602 DNA Human genes 0.000 description 29
- 108020004414 DNA Proteins 0.000 description 29
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 16
- 238000010586 diagram Methods 0.000 description 12
- 239000000523 sample Substances 0.000 description 12
- 238000004140 cleaning Methods 0.000 description 11
- 239000011324 bead Substances 0.000 description 10
- 239000008280 blood Substances 0.000 description 10
- 210000004369 blood Anatomy 0.000 description 10
- 102000039446 nucleic acids Human genes 0.000 description 9
- 108020004707 nucleic acids Proteins 0.000 description 9
- 150000007523 nucleic acids Chemical class 0.000 description 9
- 238000010521 absorption reaction Methods 0.000 description 8
- -1 polyethylene Polymers 0.000 description 8
- 239000000377 silicon dioxide Substances 0.000 description 8
- 239000007788 liquid Substances 0.000 description 7
- BASFCYQUMIYNBI-UHFFFAOYSA-N platinum Chemical compound [Pt] BASFCYQUMIYNBI-UHFFFAOYSA-N 0.000 description 6
- 238000005406 washing Methods 0.000 description 6
- 239000000356 contaminant Substances 0.000 description 5
- 108090000623 proteins and genes Proteins 0.000 description 5
- 102000004169 proteins and genes Human genes 0.000 description 5
- 238000011084 recovery Methods 0.000 description 5
- 239000000126 substance Substances 0.000 description 5
- QKNYBSVHEMOAJP-UHFFFAOYSA-N 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydron;chloride Chemical compound Cl.OCC(N)(CO)CO QKNYBSVHEMOAJP-UHFFFAOYSA-N 0.000 description 4
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 4
- 239000004696 Poly ether ether ketone Substances 0.000 description 4
- 239000004743 Polypropylene Substances 0.000 description 4
- 238000000862 absorption spectrum Methods 0.000 description 4
- 239000012472 biological sample Substances 0.000 description 4
- 239000003153 chemical reaction reagent Substances 0.000 description 4
- 238000010828 elution Methods 0.000 description 4
- 239000000203 mixture Substances 0.000 description 4
- 229920002530 polyetherether ketone Polymers 0.000 description 4
- 229920001155 polypropylene Polymers 0.000 description 4
- 238000000746 purification Methods 0.000 description 4
- PXGOKWXKJXAPGV-UHFFFAOYSA-N Fluorine Chemical compound FF PXGOKWXKJXAPGV-UHFFFAOYSA-N 0.000 description 3
- 239000004698 Polyethylene Substances 0.000 description 3
- RTAQQCXQSZGOHL-UHFFFAOYSA-N Titanium Chemical compound [Ti] RTAQQCXQSZGOHL-UHFFFAOYSA-N 0.000 description 3
- 238000004220 aggregation Methods 0.000 description 3
- 230000002776 aggregation Effects 0.000 description 3
- 239000013592 cell lysate Substances 0.000 description 3
- 238000006911 enzymatic reaction Methods 0.000 description 3
- 229910052731 fluorine Inorganic materials 0.000 description 3
- 239000011737 fluorine Substances 0.000 description 3
- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 description 3
- 239000010931 gold Substances 0.000 description 3
- 229910052737 gold Inorganic materials 0.000 description 3
- 239000002245 particle Substances 0.000 description 3
- 229910052697 platinum Inorganic materials 0.000 description 3
- 229920000573 polyethylene Polymers 0.000 description 3
- 229910052719 titanium Inorganic materials 0.000 description 3
- 239000010936 titanium Substances 0.000 description 3
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 2
- 238000002965 ELISA Methods 0.000 description 2
- KFZMGEQAYNKOFK-UHFFFAOYSA-N Isopropanol Chemical compound CC(C)O KFZMGEQAYNKOFK-UHFFFAOYSA-N 0.000 description 2
- 108091005804 Peptidases Proteins 0.000 description 2
- 239000004365 Protease Substances 0.000 description 2
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 description 2
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 2
- 238000002835 absorbance Methods 0.000 description 2
- 229910045601 alloy Inorganic materials 0.000 description 2
- 239000000956 alloy Substances 0.000 description 2
- 239000000427 antigen Substances 0.000 description 2
- 102000036639 antigens Human genes 0.000 description 2
- 108091007433 antigens Proteins 0.000 description 2
- 238000010411 cooking Methods 0.000 description 2
- 238000000605 extraction Methods 0.000 description 2
- 230000002068 genetic effect Effects 0.000 description 2
- 229960000789 guanidine hydrochloride Drugs 0.000 description 2
- PJJJBBJSCAKJQF-UHFFFAOYSA-N guanidinium chloride Chemical compound [Cl-].NC(N)=[NH2+] PJJJBBJSCAKJQF-UHFFFAOYSA-N 0.000 description 2
- 238000007689 inspection Methods 0.000 description 2
- 230000002093 peripheral effect Effects 0.000 description 2
- 238000007747 plating Methods 0.000 description 2
- 238000003752 polymerase chain reaction Methods 0.000 description 2
- 229920001296 polysiloxane Polymers 0.000 description 2
- 239000000047 product Substances 0.000 description 2
- 235000019419 proteases Nutrition 0.000 description 2
- 230000017854 proteolysis Effects 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- 230000004544 DNA amplification Effects 0.000 description 1
- 108010067770 Endopeptidase K Proteins 0.000 description 1
- 229920001213 Polysorbate 20 Polymers 0.000 description 1
- 239000013504 Triton X-100 Substances 0.000 description 1
- 229920004890 Triton X-100 Polymers 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 230000007797 corrosion Effects 0.000 description 1
- 238000005260 corrosion Methods 0.000 description 1
- 230000006378 damage Effects 0.000 description 1
- 238000006731 degradation reaction Methods 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 239000003480 eluent Substances 0.000 description 1
- 238000004049 embossing Methods 0.000 description 1
- 239000000284 extract Substances 0.000 description 1
- 238000007429 general method Methods 0.000 description 1
- 230000002934 lysing effect Effects 0.000 description 1
- 150000002739 metals Chemical class 0.000 description 1
- 229910001172 neodymium magnet Inorganic materials 0.000 description 1
- 238000001821 nucleic acid purification Methods 0.000 description 1
- 239000000256 polyoxyethylene sorbitan monolaurate Substances 0.000 description 1
- 235000010486 polyoxyethylene sorbitan monolaurate Nutrition 0.000 description 1
- 239000012521 purified sample Substances 0.000 description 1
- 229920005989 resin Polymers 0.000 description 1
- 239000011347 resin Substances 0.000 description 1
- 229920002050 silicone resin Polymers 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- 229910001256 stainless steel alloy Inorganic materials 0.000 description 1
- 238000010561 standard procedure Methods 0.000 description 1
- 239000006228 supernatant Substances 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Images
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B03—SEPARATION OF SOLID MATERIALS USING LIQUIDS OR USING PNEUMATIC TABLES OR JIGS; MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
- B03C—MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
- B03C1/00—Magnetic separation
- B03C1/02—Magnetic separation acting directly on the substance being separated
- B03C1/30—Combinations with other devices, not otherwise provided for
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
- B01F33/00—Other mixers; Mixing plants; Combinations of mixers
- B01F33/45—Magnetic mixers; Mixers with magnetically driven stirrers
- B01F33/452—Magnetic mixers; Mixers with magnetically driven stirrers using independent floating stirring elements
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B03—SEPARATION OF SOLID MATERIALS USING LIQUIDS OR USING PNEUMATIC TABLES OR JIGS; MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
- B03C—MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
- B03C1/00—Magnetic separation
- B03C1/02—Magnetic separation acting directly on the substance being separated
- B03C1/28—Magnetic plugs and dipsticks
- B03C1/288—Magnetic plugs and dipsticks disposed at the outer circumference of a recipient
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B03—SEPARATION OF SOLID MATERIALS USING LIQUIDS OR USING PNEUMATIC TABLES OR JIGS; MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
- B03C—MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
- B03C2201/00—Details of magnetic or electrostatic separation
- B03C2201/18—Magnetic separation whereby the particles are suspended in a liquid
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B03—SEPARATION OF SOLID MATERIALS USING LIQUIDS OR USING PNEUMATIC TABLES OR JIGS; MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
- B03C—MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
- B03C2201/00—Details of magnetic or electrostatic separation
- B03C2201/26—Details of magnetic or electrostatic separation for use in medical or biological applications
Definitions
- the present invention will be described by taking DNA extraction using magnetic particles as an example.
- DNA extraction from a biological sample a method using magnetic particles is useful.
- magnetic particles are likely to aggregate, particularly due to blood-derived components. For example, aggregation of magnetic particles occurs due to proteins that are contaminant components in the sample.
- the magnetic particles are aggregated, there is a problem that due to insufficient washing, the purity of the extracted DNA is likely to decrease and the recovery rate is likely to decrease due to elution failure.
- the magnetic particle dispersion method and the magnetic particle dispersion device of the above examples function efficiently in cleaning.
Landscapes
- Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Mixers With Rotating Receptacles And Mixers With Vibration Mechanisms (AREA)
Abstract
La présente invention concerne un dispositif de dispersion de particules magnétiques qui disperse des particules magnétiques agglomérées et est pourvu : d'un élément de séparation ayant des ouvertures plus grandes que chaque particule magnétique ; une structure de type bateau dans laquelle est disposé l'élément de séparation ; un mécanisme de génération de champ magnétique pour la dispersion des particules magnétiques agglomérées par application d'un champ magnétique aux particules magnétiques agglomérées retenues dans la structure de type bateau, de sorte que les particules magnétiques agglomérées soient soumises à une abrasion sur la surface de l'élément de séparation. Les particules magnétiques dispersées sont collectées à travers les ouvertures de l'élément de séparation.
Priority Applications (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2016505994A JP6233498B2 (ja) | 2014-03-05 | 2014-03-05 | 磁性体粒子分散方法及び磁性体粒子分散装置 |
| PCT/JP2014/055588 WO2015132898A1 (fr) | 2014-03-05 | 2014-03-05 | Procédé de dispersion de particules magnétiques et dispositif de dispersion de particules magnétiques |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| PCT/JP2014/055588 WO2015132898A1 (fr) | 2014-03-05 | 2014-03-05 | Procédé de dispersion de particules magnétiques et dispositif de dispersion de particules magnétiques |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| WO2015132898A1 true WO2015132898A1 (fr) | 2015-09-11 |
Family
ID=54054735
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| PCT/JP2014/055588 WO2015132898A1 (fr) | 2014-03-05 | 2014-03-05 | Procédé de dispersion de particules magnétiques et dispositif de dispersion de particules magnétiques |
Country Status (2)
| Country | Link |
|---|---|
| JP (1) | JP6233498B2 (fr) |
| WO (1) | WO2015132898A1 (fr) |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2019529063A (ja) * | 2016-07-28 | 2019-10-17 | メディシーブ リミテッド | 磁性混合器および方法 |
| CN111849766A (zh) * | 2020-09-03 | 2020-10-30 | 杭州天微基因科技有限公司 | 一种核酸提取扩增检测一体化分析仪 |
| CN112358965A (zh) * | 2020-11-09 | 2021-02-12 | 中国计量大学 | 一种磁珠法核酸提取设备 |
| CN113088430A (zh) * | 2021-04-09 | 2021-07-09 | 广州新成生物科技有限公司 | 一种旋转式吸磁装置 |
| EP4115981A1 (fr) * | 2021-07-09 | 2023-01-11 | Miltenyi Biotec B.V. & Co. KG | Séparation magnétique avec champ magnétique rotatif/colonne rotative |
| US11828691B2 (en) * | 2017-01-27 | 2023-11-28 | Dh Technologies Development Pte. Ltd. | Electromagnetic assemblies for processing fluids |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS51105158A (fr) * | 1975-03-12 | 1976-09-17 | Miura Eng Int | |
| US4000062A (en) * | 1975-04-21 | 1976-12-28 | Minnesota Mining And Manufacturing Company | Magnetic particle separator |
| JP2002538458A (ja) * | 1999-03-02 | 2002-11-12 | クオリジエン・インコーポレイテツド | 生物学的流体の分離のための装置を用いる方法 |
| JP2006007003A (ja) * | 2004-06-22 | 2006-01-12 | Canon Semiconductor Equipment Inc | 粉体供給装置 |
| JP2011232260A (ja) * | 2010-04-28 | 2011-11-17 | Shimadzu Corp | ゲル状液滴封入媒体を用いたデバイス及び液滴操作方法 |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE102005046146A1 (de) * | 2005-02-25 | 2006-08-31 | Bayerische Motoren Werke Ag | Teilintegralbremse für ein Motorrad |
-
2014
- 2014-03-05 JP JP2016505994A patent/JP6233498B2/ja active Active
- 2014-03-05 WO PCT/JP2014/055588 patent/WO2015132898A1/fr active Application Filing
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS51105158A (fr) * | 1975-03-12 | 1976-09-17 | Miura Eng Int | |
| US4000062A (en) * | 1975-04-21 | 1976-12-28 | Minnesota Mining And Manufacturing Company | Magnetic particle separator |
| JP2002538458A (ja) * | 1999-03-02 | 2002-11-12 | クオリジエン・インコーポレイテツド | 生物学的流体の分離のための装置を用いる方法 |
| JP2006007003A (ja) * | 2004-06-22 | 2006-01-12 | Canon Semiconductor Equipment Inc | 粉体供給装置 |
| JP2011232260A (ja) * | 2010-04-28 | 2011-11-17 | Shimadzu Corp | ゲル状液滴封入媒体を用いたデバイス及び液滴操作方法 |
Cited By (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2019529063A (ja) * | 2016-07-28 | 2019-10-17 | メディシーブ リミテッド | 磁性混合器および方法 |
| JP7071040B2 (ja) | 2016-07-28 | 2022-05-18 | メディシーブ リミテッド | 磁性混合器および方法 |
| US11828691B2 (en) * | 2017-01-27 | 2023-11-28 | Dh Technologies Development Pte. Ltd. | Electromagnetic assemblies for processing fluids |
| CN111849766A (zh) * | 2020-09-03 | 2020-10-30 | 杭州天微基因科技有限公司 | 一种核酸提取扩增检测一体化分析仪 |
| CN112358965A (zh) * | 2020-11-09 | 2021-02-12 | 中国计量大学 | 一种磁珠法核酸提取设备 |
| JP6994150B1 (ja) * | 2020-11-09 | 2022-02-04 | 中国計量大学 | 核酸抽出装置 |
| JP2022076437A (ja) * | 2020-11-09 | 2022-05-19 | 中国計量大学 | 核酸抽出装置 |
| CN112358965B (zh) * | 2020-11-09 | 2023-01-24 | 中国计量大学 | 一种磁珠法核酸提取设备 |
| CN113088430A (zh) * | 2021-04-09 | 2021-07-09 | 广州新成生物科技有限公司 | 一种旋转式吸磁装置 |
| CN113088430B (zh) * | 2021-04-09 | 2024-07-19 | 广州新成生物科技有限公司 | 一种旋转式吸磁装置 |
| EP4115981A1 (fr) * | 2021-07-09 | 2023-01-11 | Miltenyi Biotec B.V. & Co. KG | Séparation magnétique avec champ magnétique rotatif/colonne rotative |
Also Published As
| Publication number | Publication date |
|---|---|
| JP6233498B2 (ja) | 2017-11-22 |
| JPWO2015132898A1 (ja) | 2017-03-30 |
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