WO2015132898A1 - Method for dispersing magnetic particles and magnetic particle dispersing device - Google Patents

Abstract

This magnetic particle dispersing device disperses aggregated magnetic particles and is provided with: a separation member having openings larger than each magnetic particle; a vessel-like structure having the separation member arranged therein; a magnetic field-generating mechanism for dispersing the aggregated magnetic particles by applying a magnetic field to the aggregated magnetic particles retained in the vessel-like structure so that the aggregated magnetic particles are subject to abrasion on the surface of the separation member. The dispersed magnetic particles are collected through the openings of the separation member.

Description

Magnetic particle dispersion method and magnetic particle dispersion device

The present invention relates to a magnetic particle dispersion method and a magnetic particle dispersion device, and more particularly to a magnetic particle dispersion method and a magnetic particle dispersion device for dispersing aggregated magnetic particles.

In order to separate, purify, and detect a specific component from a biological sample using magnetic particles, it is necessary to separate the magnetic particles holding the target component from the contaminant components. For example, an example of extracting DNA (deoxyribonucleic acid) from blood is given. First, a cell lysate containing protease is mixed with a sample. DNA is released into the liquid by cell destruction and protein degradation. Next, the target DNA is adsorbed on silica magnetic beads or the like. Wash away any contaminating components. Finally, DNA is eluted from the magnetic particles and collected.

For example, as described in Patent Document 1, in a nucleic acid purification method for the purpose of genetic testing, nucleic acids can be easily extracted without using harmful reagents by utilizing the property that nucleic acids specifically adsorb to silica. And a purification method is employed. In particular, a purification method using magnetic particles having a silica surface is advantageous for automation, and is commercially available as a nucleic acid extraction and purification apparatus from various companies.

In such a device, the sample is dissolved with a chemical solution to release nucleic acid, and magnetic particles are added thereto to specifically adsorb the nucleic acid on the silica surface, and the contaminant component is removed in the subsequent washing step. Thereafter, it is possible to obtain a nucleic acid purified sample through a step of collecting only the nucleic acid. In a genetic test, a gene amplification method represented by PCR (polymerase chain reaction) is performed using nucleic acids collected from such an apparatus.

In addition, in the ELISA method (Enzyme-Linked ImmunoSorbent Assay), which is a method used to detect and quantify the concentrations of antibodies and antigens contained in a sample, magnetic particles having a binding surface with a specific component were used. The method is also advantageous for building an automatic inspection system.

JP 2011-232260 A

For example, when a specific component is separated from a biological sample such as blood, an agglomerate in which magnetic particles are difficult to disperse is formed by a contaminating component such as a protein in the sample. As a result, there has been a problem that the removal of contaminating components from the magnetic particles holding the target component and the subsequent elution of the target component are hindered, and the purity and recovery rate of the recovered target component are reduced.

The object of the present invention is to disperse agglomerated magnetic particles.

A magnetic particle dispersion method according to the present invention is a method for dispersing aggregated magnetic particles, and a container-like structure in which a partition member having a larger opening than the magnetic particles is disposed is provided. The step of containing the agglomerated magnetic particles, and applying a magnetic field to the agglomerated magnetic particles to scrape the agglomerated magnetic particles on the surface of the partition member to disperse the agglomerated magnetic particles. Recovering the dispersed magnetic particles through the openings.

A magnetic particle dispersing apparatus according to the present invention is an apparatus for dispersing agglomerated magnetic particles, wherein a partition member having an opening larger than the magnetic particles and the partition member are disposed inside. The agglomerated magnetic particles obtained by rubbing the agglomerated magnetic particles on the surface of the partition member by applying a magnetic field to the container-like structure and the agglomerated magnetic particles accommodated in the container-like structure. And a magnetic field generation mechanism for dispersing.

The magnetic particle dispersion method and the magnetic particle dispersion device of the present invention can disperse the agglomerated magnetic particles.

It is a schematic diagram for demonstrating one Example of a magnetic body particle dispersion apparatus. It is a schematic diagram for demonstrating the whole structure of the Example. It is a plane schematic diagram for demonstrating the magnetic field generation mechanism of the Example. It is a figure which shows the absorption curve of DNA with which dispersion operation was performed by the Example, and the absorption curve of DNA with which dispersion operation was performed by the normal method. It is a schematic diagram for demonstrating the other Example of a magnetic body particle dispersion apparatus. It is a schematic diagram for demonstrating the whole structure of the Example. It is a schematic diagram for demonstrating the other example of a partition member. It is a schematic diagram for demonstrating operation of DNA extraction using a magnetic body particle.

In the magnetic particle dispersion method and the magnetic particle dispersion device of the present invention, an example of the partition member is a network structure.
Further, in the magnetic particle dispersion method and the magnetic particle dispersion apparatus of the present invention, another example of the partition member is a grater-like structure having a protrusion on a surface on which the aggregated magnetic particles are abraded It is.
However, the partition member is not limited to the mesh structure and the grater-like structure.

In the magnetic particle dispersion method and the magnetic particle dispersion apparatus of the present invention, examples of the material of the partition member are synthetic resin, metal, silicon, glass, ceramics, or a combination thereof. Examples of the synthetic resin include polyethylene, polypropylene, fluorine, silicone, and PEEK (polyether ether ketone). Examples of the metal include metals such as titanium, stainless steel alloy, gold, and platinum, and alloys thereof. In addition, the material of the said partition member is not limited to what was mentioned above, Another material may be sufficient.

In the magnetic particle dispersion method of the present invention, for example, the agglomerated magnetic particles are scraped on the surface of the partition member by moving a magnet around the container-like structure. However, in the magnetic particle dispersion method of the present invention, the method of rubbing the aggregated magnetic particles on the surface of the partition member by applying a magnetic field to the aggregated magnetic particles is not limited to this, This method may be used.

In the magnetic particle dispersing apparatus of the present invention, an example of the magnetic field generating mechanism includes a magnet disposed around the container-like structure and a magnet moving mechanism that moves the magnet. However, the magnetic field generation mechanism is not limited to this configuration. The magnetic field generation mechanism may have any configuration as long as it can scrape the aggregated magnetic particles on the surface of the partition member by applying a magnetic field to the aggregated magnetic particles. Good.

In the examples described below, the present invention will be described by taking DNA extraction using magnetic particles as an example. In DNA extraction from a biological sample, a method using magnetic particles is useful. However, in DNA extraction from whole blood generally listed as a specimen, magnetic particles are likely to aggregate, particularly due to blood-derived components. For example, aggregation of magnetic particles occurs due to proteins that are contaminant components in the sample. When the magnetic particles are aggregated, there is a problem that due to insufficient washing, the purity of the extracted DNA is likely to decrease and the recovery rate is likely to decrease due to elution failure.

Therefore, the present invention uses a structure that allows the pulverized material to pass through, such as a net-like structure such as a mesh or a grinder for cooking, when performing cleaning and recovery operations. In the present invention, such a structure is used as a partition member having an opening larger than each magnetic particle. In the present invention, the aggregated magnetic particles are dispersed in the partition member by a magnetic field operation. In the present invention, since the aggregated magnetic particles are dispersed, the efficiency of the washing operation and the elution operation of the magnetic particles can be increased.

The material of the partition member may be inactive so long as it does not hinder the characteristics of the chemical used, the enzyme reaction, and the like. For example, polyethylene, polypropylene, PEEK, fluorine, silicone resin, titanium, SUS (No. 300 series), gold, platinum, plating thereof, and the like are listed as examples of the partition member. However, the material of the partition member is not limited to these as long as it is an inert material that does not hinder the characteristics of the chemicals used, the enzyme reaction, and the like.

The size of the opening in the partition member may be larger than that of the magnetic particles. The material of the partition member and the arrangement, shape, and size of the apertures are preferably selected in accordance with the state of the aggregated magnetic particles. However, the material of the partition member and the arrangement, shape, and size of the openings are not limited to being selected according to the state of the aggregated magnetic particles. The opening of the partition member may be at least as large as the individual magnetic particles in which the aggregated magnetic particles are dispersed can pass. Moreover, the partition member may be configured by overlapping a plurality of members having openings.

In the present invention, the magnetic particles that pass through the openings of the partition member are not limited to individual magnetic particles. In the present invention, the magnetic particles passing through the apertures of the partition member may include agglomerated magnetic particles that are dispersed by agglomerating the magnetic particles. Even in this case, the purity and recovery rate of the target component are improved as compared with the case where the aggregated magnetic particles are not dispersed.

Further, the size of the opening of the partition member is preferably smaller than the aggregated magnetic particles. However, the size of the opening of the partition member may be larger than the aggregated magnetic particles. Even in this case, the aggregated magnetic particles are dispersed by rubbing on the surface of the partition member.

For example, when the partition member is formed of a mesh (network structure) and the magnetic particles are magnetic beads, the size of the mesh openings (openings) may be matched to the diameter of the magnetic beads. For example, when the diameter of the magnetic beads is 0.1 to 5 μm (micrometer), an example is used in which a plurality of partition members having a mesh size of about 500 mesh (aperture 20 μm) are used.

The partition member is disposed as a partition at a position that substantially bisects the solution stored in the DNA extraction container as a container-like structure, for example. For example, when the magnetic particles are manipulated by a magnetic field generated by a magnet, the aggregated magnetic particles are redispersed when rubbed on the surface of the partition member. The present invention can improve the cleaning efficiency of the magnetic particles and the recovery rate of the target component by redispersing the agglomerated magnetic particles.

FIG. 8 is a schematic diagram for explaining the operation of DNA extraction using magnetic particles. The numbers in parentheses in FIG. 8 correspond to steps (1) to (5) described below.

(1) The sample 103 is accommodated in the container 101. The sample 103 in the container 101 is pulverized.
(2) A reagent 105 for lysing cells is injected into the container 101 using an injector 107. Cells in the sample 103 are lysed.

(3) Magnetic beads 109 in which magnetic particles are coated with silica are injected into the container 101 using an injector 107. The DNA in the sample is adsorbed on the magnetic beads 109. At this time, aggregation of the magnetic beads 109 occurs due to contaminant components such as proteins in the sample.

(4) The magnetic beads 109 are separated by the magnetic force. The separated magnetic beads 109 are washed using a washing reagent 111. This washing operation is performed twice, for example.
(5) The magnetic beads 109 are separated by the magnetic force. DNA 115 is extracted by a reagent 113 for separating the DNA.

The magnetic particle dispersion method and the magnetic particle dispersion device of the present invention are used, for example, in the operation between the steps (3) and (4) described with reference to FIG.

FIG. 1 is a schematic diagram for explaining one embodiment of a magnetic particle dispersing apparatus. FIG. 2 is a schematic diagram for explaining the overall configuration of the embodiment. FIG. 3 is a schematic plan view for explaining the magnetic field generation mechanism 7 of the embodiment.

The magnetic particle dispersion device 1 is used, for example, for DNA extraction from human whole blood using magnetic particles coated with silica. The magnetic particle dispersing apparatus 1 includes a partition member 3, a mesh fixing jig 3 a, a DNA extraction container 5 (container-like structure), a magnetic field generation mechanism 7, and a container fixing jig 9.

The partition member 3 has an opening larger than the size of the magnetic particles 11. The partition member 3 is disposed inside the DNA extraction container 5. The arrangement position of the partition member 3 is positioned by, for example, a mesh fixing jig 3a. For example, the partition member 3 is disposed as a partition at a position that substantially bisects the solution stored in the DNA extraction container 5.

The partition member 3 is formed of, for example, a mesh (network structure). The mesh stitch size of the partition member 3 is, for example, about 20 to 200 μm. The mesh stitch size of the partition member 3 is preferably changed depending on the size of the aggregate of the aggregated magnetic particles 11a to be formed. The mesh constituting the partition member 3 may be formed of a single mesh, or a plurality of meshes may be stacked. The size of the opening of the partition member 3 is not limited to these ranges as long as it is at least larger than the size of the magnetic particles 11 and has a function of dispersing the aggregated magnetic particles 11a.

The mesh material constituting the partition member 3 is, for example, a resin such as polyethylene, polypropylene, PEEK, fluorine, or silicone, a metal such as titanium, stainless alloy, gold, or platinum, or a plating thereof. The material of the partition member 3 is not limited to these as long as it is an inert material that does not inhibit corrosion by chemicals or enzyme reaction.

The magnetic field generation mechanism 7 is for applying a magnetic field to the aggregated magnetic particles 11a accommodated in the DNA extraction container 5. The magnetic field generation mechanism 7 applies a magnetic field to disperse the aggregated magnetic particles 11a by rubbing the aggregated magnetic particles 11a on the surface of the partition member 3.

The magnetic field generation mechanism 7 includes, for example, a magnet 7a, a magnet support member 7b, a balance spring 7c, a balance spring support 7d, and a fixing base 7e. The magnet support member 7b, the balance spring 7c, the balance spring support 7d, and the fixing base 7e constitute a magnet moving mechanism for moving the magnet 7a.

Two magnets 7a are provided. The magnet 7a is, for example, a neodymium magnet.
The magnet support member 7b supports the magnet 7a so that the two magnets 7a are arranged with the DNA extraction container 5 interposed therebetween. The support column of the magnet support member 7b is rotatably supported by the fixed base 7e. The magnet support member 7b is rotationally oscillated by the balance spring 7c similarly to a balance used in an analog timepiece, for example.

The balance spring 7c is for rotating and vibrating the magnet support member 7b. An end portion on the inner peripheral side of the balance spring 7c is fixed to a support column of the magnet support member 7b. The outer peripheral end of the balance spring 7c is fixed to the balance spring support 7d.
The balance spring support 7d is fixed to a fixed base 7e.

The container fixing jig 9 is for detachably supporting the DNA extraction container 5. The container fixing jig 9 is provided with a clip 9 a for sandwiching the DNA extraction container 5.
A DNA-containing solution 13 is accommodated in the DNA extraction container 5.

An operation of extracting DNA from a blood sample will be briefly described with reference to FIGS.
Protease treatment of blood samples according to standard methods. Magnetic particles 11 coated with silica are added. DNA is adsorbed on the magnetic particles 11. Aggregation of the magnetic particles 11 occurs due to contaminant components such as proteins in the blood sample, and aggregated magnetic particles 11a are formed.

The DNA-containing solution 13 containing the aggregated magnetic particles 11a is put into the DNA extraction container 5. The DNA extraction container 5 is placed on the container fixing jig 9 of the magnetic particle dispersing apparatus 1.

The magnet 7a is swung several times along the DNA extraction container 5. For example, when the operator repels the magnet 7a using a finger or the like, the magnet 7a and the magnet support member 7b are rotated and vibrated by the elasticity of the balance spring 7c. The magnet 7a and the magnet support member 7b are swung several times. The aggregated magnetic particles 11a are rubbed on the surface of the partition member 3 by the magnetic field generated by the magnet 7a. Thereby, the aggregated magnetic particles 11a are dispersed.

The dispersed magnetic particles 11 are collected by passing through the openings of the partition member 3 using the magnet 7a. After removing the supernatant of the solution, the same operation as that when the agglomerated magnetic particles 11a are rubbed on the surface of the partition member 3 is repeated using two kinds of cleaning liquids described later.

Remove the DNA extraction container 5 from the magnetic particle dispersion device 1. As a final step, the washed magnetic particles 11 are dispersed in the DNA eluate, and the DNA is eluted from the magnetic particles 11 in the solution. The DNA eluate is recovered after removing the magnetic particles 11 by magnet operation.

An example of more specific operation conditions will be described.
First, using another container, 0.01 mL of proteinase K (Roche product) was added at a concentration of 0.5 mg / mL (milligram / milliliter) to 200 μL of human whole blood (microliter). Another container is, for example, a polypropylene tube having a capacity of 1.5 mL (milliliter).

To the solution, 0.4 mL of a cell lysate was added, and a protein degradation reaction was performed at 68 ° C. for 5 minutes. The composition of the cell lysate is, for example, 30 mM Tris-HCl buffer pH 8.0, 30 mM EDTA, 5% Tween-20, 0.5% Triton X-100, 800 mM guanidine hydrochloride.

After the reaction, 100 μL of silica magnetic particles for genomic DNA extraction (5 mg / mL (Toyobo product)) suspended in isopropanol was added and mixed for 5 minutes. The solution was transferred to the DNA extraction container 5 shown in FIGS. 1 and 2, and the dispersed magnetic particles 11a were dispersed, the magnetic particles 11 were washed, and the DNA was eluted.

Two types of cleaning liquids A and B were used as the cleaning liquid.
The composition of the cleaning solution A is, for example, 37% (v / v) ethanol, 4.8M guanidine hydrochloride, 20 mM Tris-HCl buffer.
The composition of the washing solution B is, for example, 80% (v / v) ethanol, 20 mM sodium chloride, 2 mM Tris-HCl buffer pH 7.6.
Each cleaning operation was performed using 0.5 mL each of the cleaning liquid A and the cleaning liquid B.

Finally, DNA elution was performed using the DNA eluate. The composition of the DNA eluate is, for example, 10 mM Tris-HCl, pH 8.0, 1 mM EDTA.

DNA extracted from human whole blood using the DNA extraction container 5 shown in FIGS. 1 and 2 was analyzed by ultraviolet absorption spectrum.
FIG. 4 is a graph showing an absorption curve of DNA subjected to a dispersion operation according to an example and an absorption curve of DNA subjected to a dispersion operation according to a normal method. In FIG. 4, the absorption curve of the example is an absorption curve of DNA extracted by the DNA extraction operation including the example of the magnetic particle dispersion operation. The absorption curve of the normal method is an absorption curve of DNA extracted by a general technique in which magnetic particles collected by a magnet are dispersed with a micropipette using a container that does not have the partition member 3.

In the dispersion operation of magnetic particles in a general method, the agglomerated magnetic particles are dispersed by the water flow generated by the micropipette using the cleaning liquids A and B and the eluent.

On the other hand, in the dispersion operation of the aggregated magnetic particles 11a in the above-described embodiment, the operation is performed as described above using the DNA extraction container 5 in which the partition member 3 shown in FIGS.

The ultraviolet absorption spectrum of the DNA extract obtained by each method is compared. In the usual method, the purity value by absorbance 260/280 was 1.479. On the other hand, in the method of the example, the purity value by absorbance 260/280 was 1.793.

Compare the shape of the absorption spectrum curve. In the method of the example, a peak at a wavelength of 260 nm specific to DNA can be clearly confirmed. On the other hand, in the normal method, the peak at a wavelength of 260 nm is shoulder-shaped. From the shape of the absorption spectrum curve, it can be seen that the conventional method contains a large amount of residual low molecules.

Thus, it can be seen that the magnetic particle dispersion method and the magnetic particle dispersion device of the above examples function efficiently in cleaning.

FIG. 5 is a schematic view for explaining another embodiment of the magnetic particle dispersing apparatus. FIG. 6 is a schematic diagram for explaining the overall configuration of the embodiment. 5 and 6, the same reference numerals are given to portions that perform the same functions as those in FIGS. 1, 2, and 3.

In the magnetic particle dispersing apparatus 1 of this embodiment, the partition member 3 made of, for example, a mesh is fixed in the DNA extraction container 5 by one mesh fixing jig 3a. In the present invention, the partition members arranged inside the container-like structure may be arranged in any manner as long as the aggregated magnetic particles are dispersed.

For example, the DNA extraction container 5 is held obliquely by the container fixing jig 9.
The magnetic field generation mechanism 7 is fixed by rotating, for example, 90 degrees as compared with the arrangement of the magnetic field generation mechanism 7 shown in FIG. The magnet 7a is rotationally vibrated along the wall surface of the DNA extraction container 5 in the vicinity of the DNA extraction container 5.

The magnetic particle dispersing apparatus 1 of this embodiment is similar to the magnetic particle apparatus described with reference to FIGS. 1, 2 and 3 by applying a magnetic field to the aggregated magnetic particles 11a. The magnetic particles 11a aggregated on the surface of the partition member 3 can be scraped and dispersed.

In the above embodiment, a mesh is used as the partition member 3, but the partition member is not limited to a mesh in the present invention.

FIG. 7 is a schematic diagram for explaining another example of the partition member.
The partition member 15 includes a plurality of apertures 15a and a plurality of protrusions 15b. The opening 15a is formed by a through hole. The protrusion 15b is disposed on the surface on which the aggregated magnetic particles are abraded. The partition member 15 has a grater-like structure body, such as a grinder for cooking.

The partition member 15 has a large number of protrusions 15b on one surface, for example. The opening 15a is for allowing the pulverized material formed by rubbing the agglomerated magnetic particles to pass therethrough.

The material of the partition member 15 is, for example, metal, here, SUS (No. 300 series). For example, the opening 15a and the protrusion 15b of the partition member 15 are formed by embossing. However, the material and forming method of the partition member 15 are not limited to this.

As mentioned above, although the Example of this invention was described, the structure, arrangement | positioning, material, a numerical value, etc. in an Example are examples, This invention is not limited to this, This invention described in the claim Various changes can be made within the range.

For example, in the above embodiment, the partition member 3 having a net-like structure or the partition member 15 having a grater-like structure is used, but the partition member is not limited to these in the present invention. In the present invention, the partition member has a larger opening than the magnetic particles used, and has a structure capable of dispersing the aggregated magnetic particles by rubbing the aggregated magnetic particles on the surface. Any structure may be used.

In the above embodiment, the container 5 for DNA extraction is used as the container-like structure, but the container-like structure is not limited to this in the present invention. In the present invention, the material and structure of the container-like structure are not particularly limited as long as the partition member can be disposed inside.

Moreover, in the said Example, the magnet 7a arrange | positioned around the container 5 for DNA extraction, and the magnet which consists of the magnet support member 7b for moving the magnet 7a, the balance spring 7c, the balance spring support 7d, and the fixing stand 7e. A magnetic field generating mechanism 7 having a moving mechanism is used. However, in the magnetic particle dispersing apparatus of the present invention, the magnetic field generation mechanism is not limited to the magnetic field generation mechanism 7 of the embodiment.

In the magnetic particle dispersing apparatus of the present invention, the magnetic field generation mechanism disperses the magnetic particles aggregated on the surface of the partition member by applying a magnetic field to the aggregated magnetic particles accommodated in the container-like structure. Any configuration is possible as long as it can be configured.

For example, the drive source for moving the magnet 7a may be different from the balance spring 7c, for example, a motor or the like. The movement of the magnet 7a is not limited to rotational vibration. For example, the movement of the magnet 7a may be sliding.

Further, instead of the magnet 7a which is a permanent magnet, an electromagnet that generates a magnetic field electrically may be used. For example, if a plurality of electromagnets are arranged around the container-like structure and the electromagnets are controlled so as to generate magnetic fields at different timings, they aggregate on the surface of the partition member even if the electromagnets are not moved. The magnetic particles can be abraded.

In the magnetic particle dispersion method of the present invention, the element for applying a magnetic field to the aggregated magnetic particles is not limited to the magnet 7a, and may be an element that generates a magnetic field electrically, such as an electromagnet. .

Further, although the above embodiment has been described by taking a DNA extraction operation using magnetic particles as an example, the operation to which the present invention is applied is not limited to the DNA extraction operation. The present invention can be applied to, for example, extraction, purification and detection of chemical components using magnetic particles, and an apparatus for realizing the method. For example, the present invention can be used in a medical or food inspection system or a pretreatment system for separating nucleic acids, proteins, antigens, antibodies, and other biomolecules from a biological sample using magnetic particles. .

DESCRIPTION OF SYMBOLS 1 Magnetic particle dispersion device 3 Partition member 5 DNA extraction container (container-like structure)
7 Magnetic field generation mechanism 7a Magnet 11a Aggregated magnetic particles 11 Magnetic particles 15 Partition member 15a Open hole 15b Protrusion

Claims (8)

1. A method for dispersing agglomerated magnetic particles,
   Accommodating the agglomerated magnetic particles in a container-like structure in which a partition member having an opening larger than each of the magnetic particles is disposed;
   By applying a magnetic field to the agglomerated magnetic particles, the agglomerated magnetic particles are scraped on the surface of the partition member to disperse the agglomerated magnetic particles, and the dispersed magnetic particles are passed through the openings. And collecting the magnetic particles.
2. 2. The magnetic particle dispersion method according to claim 1, wherein the partition member is a grate-like structure having a net-like structure or a protrusion on a surface on which the agglomerated magnetic particles are abraded.
3. 3. The magnetic particle dispersion method according to claim 1, wherein the partition member is made of synthetic resin, metal, silicon, glass, ceramics, or a combination thereof.
4. The magnetic particle dispersion method according to any one of claims 1 to 3, wherein the agglomerated magnetic particles are scraped on the surface of the partition member by moving a magnet around the container-like structure.
5. An apparatus for dispersing agglomerated magnetic particles,
   A partition member having an opening larger than the magnetic particles;
   A container-like structure in which the partition member is disposed;
   A magnetic field for dispersing the agglomerated magnetic particles by rubbing the agglomerated magnetic particles on the surface of the partition member by applying a magnetic field to the agglomerated magnetic particles accommodated in the container-like structure. And a magnetic particle dispersion device.
6. 6. The magnetic particle dispersing apparatus according to claim 5, wherein the partition member is a net-like structure or a grater-like structure having a protrusion on a surface on which the agglomerated magnetic particles are abraded.
   
7. The magnetic particle dispersion device according to claim 5 or 6, wherein the material of the partition member is synthetic resin, metal, silicon, glass, ceramics, or a combination thereof.
8. 8. The magnetic particle dispersion according to claim 5, wherein the magnetic field generation mechanism includes a magnet disposed around the container-like structure and a magnet moving mechanism that moves the magnet. apparatus.

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