CN112358965B - Magnetic bead method nucleic acid extraction equipment - Google Patents
Magnetic bead method nucleic acid extraction equipment Download PDFInfo
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- CN112358965B CN112358965B CN202011238142.3A CN202011238142A CN112358965B CN 112358965 B CN112358965 B CN 112358965B CN 202011238142 A CN202011238142 A CN 202011238142A CN 112358965 B CN112358965 B CN 112358965B
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- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
- C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
- C12N15/1013—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by using magnetic beads
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Abstract
The invention discloses nucleic acid extraction equipment by a magnetic bead method, which comprises a bottom plate, a vertical plate arranged on the bottom plate and an extraction tube arranged on the bottom plate, wherein the bottom plate is provided with a mounting groove, the extraction tube is inserted in the mounting groove, the bottom of the extraction tube is provided with a plurality of liquid outlet holes, the side wall of the extraction tube is provided with a liquid inlet tube, and the bottom of the extraction tube is also provided with a sealing plate matched with the liquid outlet holes; a first sleeve is arranged in the extraction pipe in a penetrating manner, a first push plate is arranged on the first sleeve, a plurality of through grooves are formed in the first push plate, and a plurality of first baffle plates matched with the through grooves are arranged on the first push plate; the vertical plate is provided with a magnetic bar, and the vertical plate is also provided with a driving motor for driving the first sleeve to rotate.
Description
Technical Field
The invention belongs to the technical field of nuclide extraction, and particularly relates to nucleic acid extraction equipment by a magnetic bead method.
Background
In the operation of extracting nucleic acid by a magnetic bead method, whether the magnetic bead and free nucleic acid are mixed fully or not, and whether the magnetic bead is completely adsorbed by a magnetic rod or not are very important to the nucleic acid extraction effect, especially the magnetic bead mixing operation and magnetic bead adsorption of a large-volume sample. For the conventional small-volume nucleic acid extraction work, when nucleic acid is extracted, magnetic beads and free nucleic acid are uniformly mixed through the up-and-down reciprocating motion of a magnetic rod, the rotary motion of a magnetic rod sleeve and the liquid suction and discharge modes of a liquid shifter, the magnetic beads adsorb the nucleic acid, and then the magnetic rod adsorbs the magnetic beads on the outer wall of the magnetic rod sleeve, so that one step of the nucleic acid extraction work is completed.
For the work of extracting large sample volume, after adding lysis solution, buffer solution and magnetic bead solution, the total liquid volume is larger when extracting nucleic acid, a large-volume sample test tube is needed, the large-volume sample liquid and the magnetic beads are difficult to be fully mixed, and the work of completely extracting nucleic acid by using a common nucleic acid extraction instrument is difficult, namely, the work of extracting nucleic acid is not complete or the work of continuously and repeatedly mixing and extracting nucleic acid needs to be carried out by increasing the extraction time, so that the extraction rate and the extraction efficiency are influenced.
Disclosure of Invention
The invention provides a nucleic acid extraction device for improving the extraction rate of nucleic acid in order to overcome the defects of the prior art.
In order to achieve the purpose, the invention adopts the following technical scheme: a magnetic bead method nucleic acid extraction device comprises a bottom plate, a vertical plate arranged on the bottom plate and an extraction tube arranged on the bottom plate, wherein the bottom plate is provided with a mounting groove, the extraction tube is inserted in the mounting groove, the bottom of the extraction tube is provided with a plurality of liquid outlet holes, the side wall of the extraction tube is provided with a liquid inlet tube, and the bottom of the extraction tube is also provided with a sealing plate matched with the liquid outlet holes; a first sleeve is arranged in the extraction pipe in a penetrating manner, a first push plate is arranged on the first sleeve, a plurality of through grooves are formed in the first push plate, and a plurality of first baffle plates matched with the through grooves are arranged on the first push plate; the vertical plate is provided with a magnetic bar and a driving motor for driving the first sleeve to rotate; when extracting nucleic acid, putting a sample into an extraction tube, putting lysis solution into the sample, driving a first sleeve to rotate by a driving motor, driving a first push plate to rotate by the first sleeve, stirring the sample in the extraction tube by the first push plate, and allowing part of the sample to flow through the through groove when the first push plate rotates, so that the sample in the extraction tube has different flow rates, the sample and magnetic beads are more uniformly mixed, and the nucleic acid is adsorbed on the magnetic beads; after magnetic beads and a sample are uniformly mixed, a magnetic rod is inserted into a first sleeve, a driving motor drives the first sleeve to rotate, a first baffle plate seals a through groove, the first sleeve drives a first push plate to rotate, the first push plate rotates in an extraction tube, and all the magnetic beads in the extraction tube are adsorbed on the first push plate under the action of the first push plate, so that the magnetic beads in the extraction tube can be collected, and magnetic bead omission is avoided; when the magnetic beads in the extraction tube are completely adsorbed on the first push plate, the sealing plate moves downwards, and the liquid outlet hole is opened to discharge the waste liquid in the extraction tube; after all the waste liquid in the extraction tube is discharged, adding a washing buffer solution from the liquid inlet tube, pulling out the magnetic rod from the first sleeve, enabling the magnetic beads to fall off from the first push plate and the first sleeve, driving the first sleeve to rotate by the driving motor, stirring the magnetic beads, cleaning the magnetic beads in the extraction tube, and removing the magazines on the magnetic beads; after the magnetic beads are cleaned, the magnetic bar is inserted into the first sleeve, the liquid outlet hole is opened again, and the cleaning waste liquid is discharged from the extraction pipe; then, the elution buffer solution is placed into the extraction tube, the first push plate rotates to stir the magnetic beads, so that the nucleic acids are separated from the magnetic beads, when the nucleic acids are completely separated from the magnetic beads, the magnetic rod is inserted into the first sleeve, the liquid outlet hole is opened to discharge the buffer solution in the extraction tube, and the separated nucleic acids are obtained; the liquid in the extraction tube can be quickly and timely discharged from the extraction tube after each use, so that the mixing of various liquids in the extraction tube is avoided, the liquid volume is reduced, the liquid in the extraction tube is controlled within a specified range, the magnetic beads are stirred by the first push plate, the magnetic beads and the solution are fully mixed, the volume of the extraction tube does not need to be increased, and the nucleic acid extraction effect is improved; through the closed mode of first baffle with leading to the groove, make first baffle contact with all magnetic beads in the extraction tube when rotating to do abundant collection to the magnetic bead in the extraction tube, avoid the magnetic bead to omit, guarantee the extraction rate of nucleic acid.
The improved structure of the connecting device is characterized in that a first connecting disc is arranged at the top of the first sleeve, a first movable groove is formed in the bottom of the first connecting disc, the first sleeve is inserted into the first movable groove, a second sleeve is arranged in the first sleeve in a penetrating mode, a second connecting disc is arranged at the top of the second sleeve, a first supporting plate matched with the second connecting disc is arranged on the vertical plate, and the driving motor is matched with the second connecting disc in a transmission mode.
The utility model discloses a spacing groove, including first baffle, first connecting disc, second connecting disc, first baffle, first connecting disc bottom is equipped with the second activity groove, be equipped with the third connecting disc in the second activity inslot, third connecting disc bottom is equipped with the go-between, be equipped with the annular on the go-between, the annular top is equipped with first spacing groove, be equipped with first movable chamber in the first push pedal, first baffle is located in the first movable chamber, be equipped with the head rod on the first baffle, the head rod is worn to locate in the annular, the head rod top be equipped with first spacing groove matched with limiting plate.
A first through hole is formed in the top of the second movable groove, a second connecting rod matched with the first through hole is arranged on the third connecting disc, a second supporting plate is arranged on the second connecting rod, and a first supporting spring is arranged at the bottom of the second supporting plate; the vertical plate is provided with a third movable groove, the first supporting plate is arranged in the third movable groove, and the bottom of the first supporting plate is provided with a second supporting spring.
The vertical plate is provided with a first sliding groove, a first sliding block is arranged in the first sliding groove, a connecting frame is arranged on the first sliding block, a driving roller matched with the second connecting disc is arranged on the connecting frame, a connecting shaft is arranged on the connecting frame and penetrates through the driving roller, the driving motor is arranged on the connecting frame, and a driving wheel matched with the driving roller is arranged on an output shaft of the driving motor.
The mounting groove is characterized in that a second movable cavity is formed in the inner wall of the mounting groove, a third movable cavity communicated with the second movable cavity is formed in the bottom plate, a fixed block is arranged in the second movable cavity, a fixed spring is arranged on the side wall of the fixed block, a third connecting rod is arranged on the fixed block, a fourth movable groove is formed in the top of the third movable cavity, a first pushing block matched with the fourth movable groove is arranged in the third movable cavity, a first connecting plate is arranged on the first pushing block, the first connecting plate is movably connected with a fourth connecting rod, and the other end of the fourth connecting rod is movably connected onto the third connecting rod.
The first pushing block is provided with fifth movable grooves, limiting blocks are arranged in the fifth movable grooves, limiting springs are arranged on the limiting blocks, the first pushing block is provided with fourth movable cavities communicated with the fifth movable grooves, the number of the fifth movable grooves is two, and the limiting blocks in the two groups of the fifth movable grooves are connected through a connecting rope; and a second push block is arranged at the top of the fourth movable cavity in a penetrating manner, and the second push block is arranged above the connecting rope.
The bottom of the extraction tube is provided with a fifth movable cavity, the sealing plate is arranged in the fifth movable cavity, the bottom of the fifth movable cavity is provided with a first water outlet, and the top of the sealing plate is of a conical surface structure; the sealing plate is characterized in that a sealing spring is arranged at the bottom of the sealing plate, a fifth connecting rod is arranged at the bottom of the sealing plate and penetrates through the fifth movable cavity, a first connecting block is arranged at the bottom of the fifth connecting rod, and a sixth connecting rod matched with the first connecting block is arranged at the bottom of the mounting groove.
A sixth movable groove is formed in the bottom of the mounting groove, a third supporting spring is arranged in the sixth movable groove, a sixth connecting rod is arranged at the top of the third supporting spring, a first connecting groove matched with the first connecting block is formed in the sixth connecting rod, a seventh movable groove is formed in the inner wall of the first connecting groove, a second limiting plate is arranged in the seventh movable groove, and a second connecting groove matched with the second limiting plate is formed in the first connecting block; when the first pushing block is located at one end, close to the mounting groove, of the fourth movable groove, the second limiting plate moves towards the outer side of the seventh movable groove.
Be equipped with on the sixth connecting rod with the communicating eighth movable groove of seventh movable groove, be equipped with the second connecting plate on the second limiting plate, the second connecting plate is located in the eighth movable groove, be equipped with the second spout on the eighth movable inslot wall, be equipped with on the second connecting plate lateral wall with second spout matched with second slider, be equipped with first reset spring on the second slider, be equipped with the eighth movable groove on the sixth movable inslot wall, be equipped with the second push pedal in the eighth movable groove, eighth movable groove one end is equipped with sixth movable chamber, sixth movable chamber be equipped with second push pedal matched with push rod.
The invention has the following advantages: the liquid in the extraction tube can be quickly and timely discharged from the extraction tube after each use, so that the mixing of various liquids in the extraction tube is avoided, the liquid volume is reduced, the liquid in the extraction tube is controlled within a specified range, the magnetic beads are stirred by using the first push plate, the magnetic beads and the solution are fully mixed, the volume of the extraction tube is not required to be increased, and the nucleic acid extraction effect is improved; through first baffle with lead to groove confined mode, make first baffle contact with all magnetic beads in the extraction tube when rotating to do abundant collection to the magnetic bead in the extraction tube, avoid the magnetic bead to omit, guarantee the extraction rate of nucleic acid.
Drawings
FIG. 1 is a schematic structural diagram of the present invention.
Fig. 2 is a front view of the present invention.
Fig. 3 isbase:Sub>A cross-sectional view taken alongbase:Sub>A-base:Sub>A in fig. 2.
Fig. 4 is an enlarged view of a portion a in fig. 3.
Fig. 5 is an enlarged view of fig. 3 at B.
Fig. 6 is an enlarged view of fig. 3 at C.
Fig. 7 is an enlarged view of fig. 6 at D.
Fig. 8 is a cross-sectional view taken along line B-B of fig. 2.
Fig. 9 is an enlarged view of fig. 8 at F.
Fig. 10 is a cross-sectional view taken along line C-C of fig. 2.
Fig. 11 is an enlarged view at G in fig. 10.
Fig. 12 is an enlarged view of fig. 10 at H.
Fig. 13 is a cross-sectional view taken along line D-D in fig. 2.
Fig. 14 is an enlarged view of fig. 13 at I.
Fig. 15 is a right side view of the present application.
Fig. 16 is a cross-sectional view taken along line E-E of fig. 15.
Fig. 17 is an enlarged view at J in fig. 16.
FIG. 18 is an enlarged view of FIG. 16 taken at K
Fig. 19 is an enlarged view at L in fig. 18.
Fig. 20 is a cross-sectional view taken along F-F in fig. 15.
Fig. 21 is an enlarged view of fig. 20 at M.
Fig. 22 is an enlarged view of fig. 21 at N.
Fig. 23 is a sectional view taken along G-G in fig. 15.
Fig. 24 is an enlarged view at O in fig. 23.
Detailed Description
As shown in fig. 1 to 24, a magnetic bead method nucleic acid extraction apparatus includes a bottom plate 1, a vertical plate 2 disposed on the bottom plate 1, and an extraction tube 4 disposed on the bottom plate 1, wherein the bottom plate 1 is provided with an installation groove 16, the extraction tube 4 is inserted into the installation groove 16, the bottom of the extraction tube 4 is provided with a plurality of liquid outlet holes 41, a liquid inlet tube 44 is disposed on a side wall of the extraction tube 4, and the bottom of the extraction tube 4 is further provided with a sealing plate 43 matched with the liquid outlet holes 41; a first sleeve 5 penetrates through the extraction pipe 4, a first push plate 54 is arranged on the first sleeve 5, a plurality of through grooves 541 are formed in the first push plate 54, and a plurality of first baffle plates 542 matched with the through grooves 541 are arranged on the first push plate 54; the vertical plate 2 is provided with a magnetic bar 9, and the vertical plate 2 is also provided with a driving motor 25 for driving the first sleeve 5 to rotate; when nucleic acid is extracted, a sample is placed into the extraction tube, then lysate is placed into the sample, the driving motor drives the first sleeve to rotate, the first sleeve drives the first push plate to rotate, the first push plate plays a role in stirring the sample in the extraction tube, when the first push plate rotates, part of the sample flows through the through groove, so that the sample in the extraction tube has different flow rates, the sample and magnetic beads are more uniformly mixed, and the nucleic acid is adsorbed on the magnetic beads; after the magnetic beads and the sample are uniformly mixed, the magnetic rod is inserted into the first sleeve, the driving motor drives the first sleeve to rotate, the through groove is sealed by the first baffle plate, the first sleeve drives the first push plate to rotate, the first push plate rotates in the extraction tube, and under the action of the first push plate, all the magnetic beads in the extraction tube are adsorbed on the first push plate so as to collect the magnetic beads in the extraction tube and avoid magnetic bead omission; when the magnetic beads in the extraction tube are completely adsorbed on the first push plate, the sealing plate moves downwards, and the liquid outlet hole is opened to discharge the waste liquid in the extraction tube; after all the waste liquid in the extraction tube is discharged, adding a washing buffer solution from the liquid inlet tube, pulling out the magnetic rod from the first sleeve, enabling the magnetic beads to fall off from the first push plate and the first sleeve, driving the first sleeve to rotate by the driving motor, stirring the magnetic beads, cleaning the magnetic beads in the extraction tube, and removing the magazines on the magnetic beads; after the magnetic beads are cleaned, the magnetic bar is inserted into the first sleeve, the liquid outlet hole is opened again, and the cleaning waste liquid is discharged from the extraction pipe; then, the elution buffer solution is placed into the extraction tube, the first push plate rotates to stir the magnetic beads, so that the nucleic acids are separated from the magnetic beads, when the nucleic acids are completely separated from the magnetic beads, the magnetic rod is inserted into the first sleeve, the liquid outlet hole is opened to discharge the buffer solution in the extraction tube, and the separated nucleic acids are obtained; the liquid in the extraction tube can be quickly and timely discharged from the extraction tube after each use, so that the mixing of various liquids in the extraction tube is avoided, the liquid volume is reduced, the liquid in the extraction tube is controlled within a specified range, the magnetic beads are stirred by the first push plate, the magnetic beads and the solution are fully mixed, the volume of the extraction tube does not need to be increased, and the nucleic acid extraction effect is improved; through first baffle with lead to groove confined mode, make first baffle contact with all magnetic beads in the extraction tube when rotating to do abundant collection to the magnetic bead in the extraction tube, avoid the magnetic bead to omit, guarantee the extraction rate of nucleic acid.
A first connecting disc 51 is arranged at the top of the first sleeve 5, a first movable groove 511 is arranged at the bottom of the first connecting disc 51, the first sleeve 5 is inserted into the first movable groove 511, a second sleeve 6 penetrates through the first sleeve 5, a second connecting disc 61 is arranged at the top of the second sleeve 6, a first supporting plate 262 matched with the second connecting disc 61 is arranged on the vertical plate 2, and the driving motor 25 is in transmission fit with the second connecting disc 61; the first connecting disc provides supporting force for the first sleeve, so that a certain distance is reserved between the bottom end of the first sleeve and the extraction pipe, the resistance force applied when the first sleeve rotates is reduced, and the first sleeve is easier to rotate; the rubber layer is arranged on the side wall of the second sleeve, and the friction force between the first sleeve and the second sleeve is increased under the arrangement of the rubber layer, so that the first sleeve is driven to rotate when the second sleeve rotates; one end of the first supporting plate is positioned at the bottom of the second connecting disc, the first supporting plate is used for providing supporting force for the second sleeve, and the second connecting disc is positioned above the first connecting disc when the magnetic rod is not inserted into the second sleeve; after the sample was put into the extraction tube, driving motor drive second connection pad rotated, and the second connection pad drives the second sleeve and rotates, drives first sleeve rotation under the second sleeve effect to utilize first push pedal to play the stirring effect to sample and magnetic bead, also utilize first push pedal to play the collection effect to the magnetic bead simultaneously.
A second movable groove 52 is formed in the bottom of the first connecting disc 51, a third connecting disc 53 is arranged in the second movable groove 52, a connecting ring 531 is arranged at the bottom of the third connecting disc 53, an annular groove 532 is formed in the connecting ring 531, a first limiting groove 533 is formed in the top of the annular groove 532, a first movable cavity is formed in the first push plate 54, the first baffle 542 is arranged in the first movable cavity, a first connecting rod 543 is arranged on the first baffle 542, the first connecting rod 543 is arranged in the annular groove 532 in a penetrating mode, and a first limiting plate 544 matched with the first limiting groove 533 is arranged at the top of the first connecting rod 543; when the driving motor drives the second connecting disc to rotate, the second connecting disc drives the second sleeve to rotate, the second sleeve drives the first sleeve to rotate, and the first push plate rotates along with the first sleeve to stir a sample in the extraction tube; when the second sleeve drives the first sleeve to rotate, the first limiting plate moves in the first limiting groove, so that the first sleeve rotates relative to the first connecting disc, the first connecting disc does not need to rotate, and the friction force generated when the first sleeve rotates is reduced; the third connection pad does not rotate along with first sleeve pipe, makes third connection pad rigidity to promote first baffle and remove under the third connection pad effect, make first baffle will lead to the groove and seal, do the collection to the magnetic bead in the extraction tube.
A first through hole is formed in the top of the second movable groove 52, a second connecting rod 534 matched with the first through hole is arranged on the third connecting disc 53, a second supporting plate 535 is arranged on the second connecting rod 534, and a first supporting spring 536 is arranged at the bottom of the second supporting plate 535; a third movable groove 26 is formed in the vertical plate 2, the first support plate 262 is arranged in the third movable groove 26, a second support spring 261 is arranged at the bottom of the first support plate 262, when liquid in the extraction tube needs to be discharged, a magnetic rod is inserted into the second sleeve, the magnetic rod pushes the second sleeve to move downwards, the second sleeve moves towards the bottom of the first sleeve, the second connecting disc moves to the top of the first connecting disc, the second connecting disc pushes the first support plate and the second support plate to move downwards, the third connecting disc is pushed to move downwards under the action of the first support plate, the third connecting disc pushes the first connecting rod to move downwards, so that the first baffle moves downwards relative to the first push plate, the first baffle moves to the through groove to seal the through groove, the driving motor drives the second connecting disc to rotate, the first push plate is fully contacted with the liquid in the extraction tube when rotating, magnetic beads in the liquid are adsorbed on the first push plate, and the collection of the magnetic beads is completed; after liquid discharges from the interior of the extraction tube, the bar magnet up moves, the second sleeve pipe up moves under the effect of first backup pad, make the second connection pad remove to first connection pad top, the second connection pad no longer extrudees the second backup pad, first supporting spring promotes the upward movement of second backup pad, the third connection pad drives the upward movement of head rod, make first baffle enter into first movable cavity, lead to the groove and resume the state of opening, so that play the stirring effect to liquid and magnetic bead in the extraction tube under first push pedal effect.
A first sliding groove 27 is formed in the vertical plate 2, a first sliding block 232 is arranged in the first sliding groove 27, a connecting frame 23 is arranged on the first sliding block 232, a driving roller 24 matched with the second connecting disc 61 is arranged on the connecting frame 23, a connecting shaft 231 is arranged on the connecting frame 23, the connecting shaft 231 penetrates through the driving roller 24, the driving motor 25 is arranged on the connecting frame 23, and a driving wheel 251 matched with the driving roller 24 is arranged on an output shaft of the driving motor 25; under the arrangement of the transmission roller, the transmission distance of the driving motor is increased, so that the second connecting disc can still form transmission fit with the driving motor after moving in the longitudinal direction; when inserting the second sleeve pipe in first sleeve pipe, toward first spout one end promotion link, the link removes to first spout one end, and the driving roller is together removed from extraction tube one side along with the link to directly insert the second sleeve pipe in first sleeve pipe, reduce the second sleeve pipe installation degree of difficulty.
A second movable cavity 120 is arranged on the inner wall of the mounting groove 16, a third movable cavity 19 communicated with the second movable cavity 120 is arranged on the bottom plate 1, a fixed block 110 is arranged in the second movable cavity 120, a fixed spring 1102 is arranged on the side wall of the fixed block 110, a third connecting rod 1101 is arranged on the fixed block 110, a fourth movable groove 191 is arranged at the top of the third movable cavity 19, a first pushing block 18 matched with the fourth movable groove 191 is arranged in the third movable cavity 19, a first connecting plate 185 is arranged on the first pushing block 18, a fourth connecting rod 186 is hinged to the first connecting plate 185, and the other end of the fourth connecting rod 186 is hinged to the third connecting rod 1101; the top of the mounting groove is provided with a coaming 13, the inner wall of the coaming is contacted with the side wall of the extraction tube, a heating block is arranged in the coaming, and when the first push plate rotates to stir the liquid in the extraction tube, the heating block in the coaming heats the extraction tube, so that the nucleic acid is more easily attached to the magnetic beads; one end of the fixed block is provided with a rubber pad, the friction force between the fixed block and the side wall of the extraction tube is increased under the arrangement of the rubber pad, and the side wall of the extraction tube is prevented from being scratched by the fixed block; when the extraction tube is installed, a first push block is pushed to one end, close to the installation groove, of the fourth movable groove, the first push block drives the fourth connecting rod to move, the fourth connecting rod pushes the third connecting rod to move towards two sides of the second movable cavity, and therefore the fixed block enters the second movable cavity, the extraction tube is directly inserted into the installation groove, and collision between the extraction tube and the fixed block is avoided; after the extraction tube is inserted into the mounting groove, the first pushing block is released, the fixing spring pushes the fixing block to move outwards, and the fixing block abuts against the side wall of the extraction tube to fix the extraction tube in the mounting groove.
The first push block 18 is provided with fifth movable grooves 188, limit blocks 189 are arranged in the fifth movable grooves 188, limit springs 1891 are arranged on the limit blocks 189, the first push block 18 is provided with fourth movable cavities 1810 communicated with the fifth movable grooves 188, the number of the fifth movable grooves 188 is two, and the limit blocks 189 in the two groups of the fifth movable grooves 188 are connected through a connecting rope 1892; a second pushing block 181 penetrates through the top of the fourth movable cavity 1810, and the second pushing block 181 is arranged above the connecting rope 1892; a seventh movable cavity 184 is arranged above the fourth movable cavity, a second push block penetrates through the seventh movable cavity, a third limiting plate 182 matched with the seventh movable cavity is arranged on the second push block, and a second reset spring 183 is arranged at the bottom of the third limiting plate; when the extraction pipe is installed, the second pushing block is pushed downwards, the middle part of the connecting rope is pushed by the second pushing block to move downwards, the limiting block is pulled by the connecting rope to move towards the fifth movable groove, and the limiting block is not abutted to the inner wall of the fourth movable groove, so that the first pushing block can move more conveniently; after the extraction tube inserted in the mounting groove, released first ejector pad and second ejector pad, second reset spring promoted the second ejector pad and up moved, and the stopper supports on fourth activity inslot wall, caused the hindrance to the removal of first ejector pad, promoted first ejector pad when avoiding the maloperation and removed, guaranteed the fixed effect of fixed block to the extraction tube.
The bottom of the extraction tube 4 is provided with a fifth movable cavity 42, the sealing plate 43 is arranged in the fifth movable cavity 42, the bottom of the fifth movable cavity 42 is provided with a first water outlet 421, the top of the sealing plate 43 is of a conical surface structure, and the bottom of the mounting groove is provided with a second water outlet 15; a sealing spring 432 is arranged at the bottom of the sealing plate 43, a fifth connecting rod 431 is arranged at the bottom of the sealing plate 43, the fifth connecting rod 431 is arranged in the fifth movable cavity 42 in a penetrating manner, a first connecting block 433 is arranged at the bottom of the fifth connecting rod 431, and a sixth connecting rod 142 matched with the first connecting block 433 is arranged at the bottom of the mounting groove 16; when the extraction pipe is inserted into the mounting groove, the first connecting block moves to the sixth connecting rod, and the first connecting block and the sixth connecting rod form connection fit; when the sample is stirred and mixed in the extraction tube, the sealing spring provides supporting force for the sealing plate, and the liquid outlet hole is sealed by the sealing plate to prevent liquid from leaking from the liquid outlet hole; after magnetic bead and nucleic acid mix the completion, the bar magnet is inserted in the second sleeve, adsorb the magnetic bead on first sleeve pipe and first push pedal under the bar magnet effect, the sixth connecting rod drives first connecting block downstream, first connecting block drives the closing plate downstream, make the liquid outlet opening, the extraction tube is discharged from liquid outlet opening department, liquid drops at the closing plate top, make liquid flow down along the closing plate surface under the conical surface structure at closing plate top, liquid drops to fifth activity chamber bottom from the closing plate, liquid in the fifth activity intracavity is discharged from first water outlet department, liquid in the mounting groove is discharged from second water outlet department.
A sixth movable groove 14 is formed in the bottom of the mounting groove 16, a third support spring 141 is arranged in the sixth movable groove 14, the sixth connecting rod 142 is arranged at the top of the third support spring 141, a driving member for driving the sixth connecting rod to move up and down is arranged on the base, the driving member is preferably an air cylinder, and the driving member is arranged in the sixth movable groove, namely below the sixth connecting rod; a first connecting groove matched with the first connecting block 433 is formed in the sixth connecting rod 142, a seventh movable groove is formed in the inner wall of the first connecting groove, a second limiting plate 1431 is arranged in the seventh movable groove, and a second connecting groove matched with the second limiting plate 1431 is formed in the first connecting block 433; when the first push block is located at one end, close to the mounting groove, of the fourth movable groove, the second limit plate 1431 moves towards the outer side of the seventh movable groove; when the extraction pipe is inserted into the mounting groove, the first connecting block is aligned with the first connecting groove, the first connecting block is inserted into the first connecting groove, the first push block is pushed towards one end, close to the mounting groove, of the fourth movable groove, the first push block is moved to drive the fixing block to move, the fixing block enters the second movable cavity, after the extraction pipe is inserted into one side of the fixing block, the first push block is pushed towards the other end of the fourth movable groove, the second fixing block cannot move by abutting against the side wall of the extraction pipe, the second limiting plate enters the seventh movable groove, the extraction pipe is continuously pushed downwards, the first connecting block is pushed into the first connecting groove, after the first connecting block is inserted into the first connecting groove, the first push block is pushed towards the direction, close to the mounting groove, the second limiting plate moves towards the outer side of the seventh movable groove, the second limiting plate is inserted into the second connecting groove, and connection between the sixth connecting plate and the first connecting block is completed; after the stirring of the liquid in the extraction tube is completed, the magnetic rod is inserted into the second sleeve, the driving piece drives the sixth connecting rod to move downwards, the sixth connecting rod drives the first connecting block to move downwards, the first connecting block drives the sealing plate to move downwards, and the liquid outlet hole is in an opening state, so that the liquid in the extraction tube is discharged, and the multiple liquid mixing is avoided to increase the liquid volume.
An eighth movable groove communicated with the seventh movable groove is formed in the sixth connecting rod 142, a second connecting plate 143 is arranged on the second limiting plate 1431, the second connecting plate 143 is arranged in the eighth movable groove, a second sliding groove 1421 is formed in the inner wall of the eighth movable groove, a second sliding block 1432 matched with the second sliding groove 1421 is arranged on the side wall of the second connecting plate 143, a first return spring 1433 is arranged on the second sliding block 1432, an eighth movable groove is formed in the inner wall of the sixth movable groove 14, a second push plate 130 is arranged in the eighth movable groove, a sixth movable cavity 150 is formed in one end of the eighth movable groove, and a push rod 140 matched with the second push plate 130 is arranged in the sixth movable cavity 150; a chute 1301 is arranged on the second push plate, a second connecting block 1401 is arranged on the push rod, and a ninth movable groove 17 communicated with the third movable cavity is arranged at the top of the sixth movable cavity; one end of the first reset spring is fixedly connected to the second sliding block, the other end of the first reset spring is fixedly connected to the inner wall of the second sliding groove, and the second limiting plate is located in the seventh movable groove when the second push plate does not push the second connecting plate; after first link block is embedded into first link tank, be close to mounting groove one end towards the fourth movable trough and promote first ejector pad, first ejector pad drives the second link block and removes, the push rod supports on the chute of second push pedal, under the mutually supporting of push rod and chute, make the push rod promote the second push pedal and remove towards the eighth movable trough outside, the second push pedal promotes the second connecting plate and removes towards eighth movable trough inner wall direction, thereby release the second limiting plate from the seventh movable trough, make the second limiting plate inlay in the second link tank, connect first link block in the sixth connecting rod, make the sixth connecting rod drive first link block and together remove when removing, control the switch that goes out the liquid hole.
The bottom of the bottom plate is provided with a supporting leg 11 and a third connecting plate 12, the bottom surface of the third connecting plate is higher than the bottom surface of the supporting leg, one side of the third connecting plate is provided with a first material receiving box 7, one side of the supporting leg is provided with a second material receiving box 8, the first material receiving box is arranged above the second material receiving box, the side wall of the first material receiving box is provided with a third sliding block 71, the third connecting plate is provided with a third sliding groove 121 matched with the third sliding block, the side wall of the second material receiving box is provided with a fourth sliding block 81, and the supporting leg is provided with a fourth sliding groove matched with the fourth sliding block; the sectional area of the second material receiving box is larger than that of the first material receiving box, the second material receiving box is directly positioned below the second water outlet, and the first material receiving box is positioned on one side of the second water outlet; when the waste liquid is discharged from the second water outlet, the waste liquid directly falls into the second material receiving box, and the waste liquid is collected by the second material receiving box; after the separation of the nucleic acid and the magnetic beads is completed, the first material receiving box moves to the position below the second water outlet, and the nucleic acid is collected in the first material receiving box when being discharged from the second water outlet, so that the nucleic acid can be collected independently, and the difficulty in collecting the nucleic acid is reduced.
A fourth connecting plate 21 is arranged on the vertical plate, a hydraulic rod 22 is arranged at the bottom of the fourth connecting plate, a mounting plate 3 is arranged on a piston rod of the hydraulic rod, a fifth chute 32 is arranged at the bottom of the mounting plate, a fifth sliding block is arranged in the fifth chute, a mounting frame 31 is arranged on the fifth sliding block, the magnetic rod is arranged in the mounting frame, a first fixing plate is arranged on the inner wall of the mounting frame, a third connecting groove matched with the first fixing plate is arranged on the magnetic rod, and the magnetic rod is fixed in the mounting frame under the mutual matching of the first fixing plate and the third connecting groove; an eighth movable cavity 36 is arranged on the mounting plate, the eighth movable cavity is of an L-shaped structure, a second baffle 33 matched with the mounting frame is arranged in the eighth movable cavity, a third push plate 331 is arranged on the second baffle, the third push plate penetrates through the eighth movable cavity, a second fixed plate 35 is arranged at the top of the eighth movable cavity, a fixed frame 34 is arranged on the third push plate, a through cavity 332 is arranged on the third push plate, and a fifth connecting plate 341 matched with the through cavity is arranged on the fixed frame; when the magnetic rod is installed, the third push plate is pushed upwards, moves to the bottom of the second fixed plate, and is fixed at the bottom of the second fixed plate under the action of the fixing frame, the third push plate drives the first fixed plate to move upwards, the first fixed plate enters the eighth movable cavity, and one end of the installation frame is in an open state, so that the magnetic rod is directly pushed into the installation frame; pulling the fixing frame outwards, enabling the fixing frame to be separated from the second fixing plate, enabling the first fixing plate to move downwards under the action of gravity, and enabling the first fixing plate to move to one side of the mounting frame to complete the mounting of the magnetic rod; when the extraction pipe, the first sleeve or the second sleeve is dismounted under the condition that the magnetic rod is mounted, the fifth sliding block is pushed to one end of the fifth sliding groove to push the magnetic rod away from the upper part of the mounting groove, so that the extraction pipe, the first sleeve or the second sleeve can be dismounted.
In order to enable the magnetic beads to fall off from the first sleeve and the first push plate more easily, the magnetic rod can adopt an electromagnet structure, and the magnetic beads automatically fall off from the first sleeve and the first push plate after the magnetic rod loses magnetism; the number of the extraction pipes can be increased according to the actual use condition, each extraction pipe can be independently used, and all the extraction pipes can also be used together; the hydraulic stem, driving motor in this application drawing are the schematic diagram, and its concrete structure is the same with hydraulic stem, motor structure among the prior art.
Claims (6)
1. The utility model provides a magnetic bead method nucleic acid extraction equipment, includes bottom plate (1), locates riser (2) on bottom plate (1) and locating extraction tube (4) on bottom plate (1), be equipped with mounting groove (16) on bottom plate (1), extraction tube (4) are inserted and are located in mounting groove (16), its characterized in that: the bottom of the extraction pipe (4) is provided with a plurality of liquid outlet holes (41), the side wall of the extraction pipe (4) is provided with a liquid inlet pipe (44), and the bottom of the extraction pipe (4) is also provided with a sealing plate (43) matched with the liquid outlet holes (41); a first sleeve (5) penetrates through the extraction pipe (4), a first push plate (54) is arranged on the first sleeve (5), a plurality of through grooves (541) are formed in the first push plate (54), and a plurality of first baffle plates (542) matched with the through grooves (541) are arranged on the first push plate (54); a magnetic bar (9) is arranged on the vertical plate (2), and a driving motor (25) for driving the first sleeve (5) to rotate is further arranged on the vertical plate (2);
the top of the first sleeve (5) is provided with a first connecting disc (51), the bottom of the first connecting disc (51) is provided with a first movable groove (511), the first sleeve (5) is inserted into the first movable groove (511), a second sleeve (6) penetrates through the first sleeve (5), the top of the second sleeve (6) is provided with a second connecting disc (61), the vertical plate (2) is provided with a first supporting plate (262) matched with the second connecting disc (61), and the driving motor (25) is in transmission fit with the second connecting disc (61);
the improved structure is characterized in that a second movable groove (52) is formed in the bottom of the first connecting disc (51), a third connecting disc (53) is arranged in the second movable groove (52), a connecting ring (531) is arranged at the bottom of the third connecting disc (53), an annular groove (532) is formed in the connecting ring (531), a first limiting groove (533) is formed in the top of the annular groove (532), a first movable cavity is formed in the first push plate (54), a first baffle (542) is arranged in the first movable cavity, a first connecting rod (543) is arranged on the first baffle (542), the first connecting rod (543) penetrates through the annular groove (532), and a first limiting plate (544) matched with the first limiting groove (533) is arranged at the top of the first connecting rod (543);
a first through hole is formed in the top of the second movable groove (52), a second connecting rod (534) matched with the first through hole is arranged on the third connecting disc (53), a second supporting plate (535) is arranged on the second connecting rod (534), and a first supporting spring (536) is arranged at the bottom of the second supporting plate (535); a third movable groove (26) is formed in the vertical plate (2), the first supporting plate (262) is arranged in the third movable groove (26), and a second supporting spring (261) is arranged at the bottom of the first supporting plate (262);
the vertical plate (2) is provided with a first sliding groove (27), a first sliding block (232) is arranged in the first sliding groove (27), a connecting frame (23) is arranged on the first sliding block (232), a driving roller (24) matched with the second connecting disc (61) is arranged on the connecting frame (23), a connecting shaft (231) is arranged on the connecting frame (23), the connecting shaft (231) penetrates through the driving roller (24), the driving motor (25) is arranged on the connecting frame (23), and a driving wheel (251) matched with the driving roller (24) is arranged on an output shaft of the driving motor (25).
2. The apparatus for extracting nucleic acid using magnetic bead method according to claim 1, wherein: be equipped with second activity chamber (120) on mounting groove (16) inner wall, be equipped with on bottom plate (1) with communicating third activity chamber (19) in second activity chamber (120), be equipped with fixed block (110) in second activity chamber (120), be equipped with fixed spring (1102) on fixed block (110) lateral wall, be equipped with third connecting rod (1101) on fixed block (110), third activity chamber (19) top is equipped with fourth activity groove (191), be equipped with in third activity chamber (19) with first ejector pad (18) of fourth activity groove (191) matched with, be equipped with first connecting plate (185) on first ejector pad (18), first connecting plate (185) swing joint has fourth connecting rod (186), fourth connecting rod (186) other end swing joint in on third connecting rod (1101).
3. The apparatus for nucleic acid isolation by magnetic bead method according to claim 2, wherein: a fifth movable groove (188) is formed in the first push block (18), a limiting block (189) is arranged in the fifth movable groove (188), a limiting spring (1891) is arranged on the limiting block (189), a fourth movable cavity (1810) communicated with the fifth movable groove (188) is formed in the first push block (18), two groups of the fifth movable grooves (188) are formed, and the limiting blocks (189) in the two groups of the fifth movable grooves (188) are connected through a connecting rope (1892); a second pushing block (181) penetrates through the top of the fourth movable cavity (1810), and the second pushing block (181) is arranged above the connecting rope (1892).
4. The apparatus for nucleic acid isolation by magnetic bead method according to claim 2, wherein: a fifth movable cavity (42) is formed in the bottom of the extraction tube (4), the sealing plate (43) is arranged in the fifth movable cavity (42), a first water outlet (421) is formed in the bottom of the fifth movable cavity (42), and the top of the sealing plate (43) is of a conical surface structure; sealing spring (432) is equipped with to closing plate (43) bottom, closing plate (43) bottom is equipped with fifth connecting rod (431), fifth connecting rod (431) wear to locate in fifth activity chamber (42), fifth connecting rod (431) bottom is equipped with first connecting block (433), mounting groove (16) bottom be equipped with first connecting block (433) matched with sixth connecting rod (142).
5. The apparatus for extracting nucleic acid using magnetic bead method according to claim 4, wherein: a sixth movable groove (14) is formed in the bottom of the mounting groove (16), a third supporting spring (141) is arranged in the sixth movable groove (14), the sixth connecting rod (142) is arranged at the top of the third supporting spring (141), a first connecting groove matched with the first connecting block (433) is formed in the sixth connecting rod (142), a seventh movable groove is formed in the inner wall of the first connecting groove, a second limiting plate (1431) is arranged in the seventh movable groove, and a second connecting groove matched with the second limiting plate (1431) is formed in the first connecting block (433); when the first push block (18) is positioned at one end of the fourth movable groove (191) close to the mounting groove (16), the second limit plate (1431) moves towards the outer side of the seventh movable groove.
6. The apparatus for extracting nucleic acid using magnetic bead method according to claim 5, wherein: an eighth movable groove communicated with the seventh movable groove is formed in the sixth connecting rod (142), a second connecting plate (143) is arranged on the second limiting plate (1431), the second connecting plate (143) is arranged in the eighth movable groove, a second sliding groove (1421) is formed in the inner wall of the eighth movable groove, a second sliding block (1432) matched with the second sliding groove (1421) is arranged on the side wall of the second connecting plate (143), a first return spring (1433) is arranged on the second sliding block (1432), an eighth movable groove is formed in the inner wall of the sixth movable groove (14), a second push plate (130) is arranged in the eighth movable groove, a sixth movable cavity (150) is formed in one end of the eighth movable groove, and a push rod (140) matched with the second push plate (130) is arranged in the sixth movable cavity (150).
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| Application Number | Priority Date | Filing Date | Title |
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| CN202011238142.3A CN112358965B (en) | 2020-11-09 | 2020-11-09 | Magnetic bead method nucleic acid extraction equipment |
| JP2021033100A JP6994150B1 (en) | 2020-11-09 | 2021-03-03 | Nucleic acid extractor |
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| Application Number | Priority Date | Filing Date | Title |
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| CN202011238142.3A CN112358965B (en) | 2020-11-09 | 2020-11-09 | Magnetic bead method nucleic acid extraction equipment |
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| CN112358965A CN112358965A (en) | 2021-02-12 |
| CN112358965B true CN112358965B (en) | 2023-01-24 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN202011238142.3A Active CN112358965B (en) | 2020-11-09 | 2020-11-09 | Magnetic bead method nucleic acid extraction equipment |
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| CN112708666B (en) * | 2020-12-17 | 2022-06-03 | 通标标准技术服务有限公司 | Magnetic separation device for detecting food-borne pathogenic bacteria by combining multiple PCR (polymerase chain reaction) with magnetic separation |
| CN112760194B (en) * | 2021-01-06 | 2022-11-25 | 常州市疾病预防控制中心 | Closed simple RNA nucleic acid extraction device and preparation method and use method thereof |
| CN115926932B (en) * | 2022-12-27 | 2023-06-13 | 上海裕隆生物科技有限公司 | Deoxyribonucleic acid extraction device in nucleic acid detection process |
| CN118163268B (en) * | 2024-04-01 | 2024-08-16 | 江苏华远路达管道科技有限公司 | Waste heat recovery type waste PE pipe hot melting recovery device |
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| WO2015132898A1 (en) * | 2014-03-05 | 2015-09-11 | 株式会社島津製作所 | Method for dispersing magnetic particles and magnetic particle dispersing device |
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| Publication number | Publication date |
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| JP6994150B1 (en) | 2022-02-04 |
| CN112358965A (en) | 2021-02-12 |
| JP2022076437A (en) | 2022-05-19 |
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