WO2015110518A1 - Procédé de fermentation comprenant une culture de cellules à faible concentration en oxygène dissous - Google Patents

Procédé de fermentation comprenant une culture de cellules à faible concentration en oxygène dissous Download PDF

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Publication number
WO2015110518A1
WO2015110518A1 PCT/EP2015/051226 EP2015051226W WO2015110518A1 WO 2015110518 A1 WO2015110518 A1 WO 2015110518A1 EP 2015051226 W EP2015051226 W EP 2015051226W WO 2015110518 A1 WO2015110518 A1 WO 2015110518A1
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WO
WIPO (PCT)
Prior art keywords
gas
bacteria
medium
coenzyme
enzyme
Prior art date
Application number
PCT/EP2015/051226
Other languages
German (de)
English (en)
Inventor
Thomas Haas
Markus PÖTTER
Eva-Maria Eckl
Simon Beck
Anja Thiessenhusen
Martin DEMLER
Original Assignee
Evonik Degussa Gmbh
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Evonik Degussa Gmbh filed Critical Evonik Degussa Gmbh
Publication of WO2015110518A1 publication Critical patent/WO2015110518A1/fr

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6409—Fatty acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/24—Preparation of oxygen-containing organic compounds containing a carbonyl group
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/40—Preparation of oxygen-containing organic compounds containing a carboxyl group including Peroxycarboxylic acids
    • C12P7/42—Hydroxy-carboxylic acids

Definitions

  • the dissolved oxygen concentration can be monitored in real time by using, for example, the above-mentioned probes.
  • Ribosome binding sites flanking the gene encoding the target enzyme.
  • foreign DNA is meant in this context any DNA sequence which is “foreign” to the gene (and not to the organism), i.e. endogenous DNA sequences may also function as “foreign DNA” in this context.
  • the carbon contained in the synthesis gas at least 50 wt .-%, preferably at least 70 wt .-%, particularly preferably at least 90 wt .-% of the carbon of all carbon sources, the

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

L'invention a pour objet un procédé comprenant une culture de bactéries méthanogènes, à une faible concentration en oxygène dissous, de 0,001 mg/L à 0,5 mg/L. Le procédé peut également comprendre la synthèse de produits cibles, qui sont choisis dans le groupe constitué par des substances dont la formation s'effectue via le produit intermédiaire acétyl-coenzyme A ou un métabolite du cycle de l'acide citrique, par exemple, acides gras, esters d'acides gras, alcools gras, aldéhydes gras, butanol, butène, propène, acétone, acide 2-hydroxyisobutyrique ou 2-propanol.
PCT/EP2015/051226 2014-01-27 2015-01-22 Procédé de fermentation comprenant une culture de cellules à faible concentration en oxygène dissous WO2015110518A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE102014201384.4 2014-01-27
DE102014201384.4A DE102014201384A1 (de) 2014-01-27 2014-01-27 Verfahren umfassend eine Kultivierung unter niedriger Gelöstsauerstoffkonzentration

Publications (1)

Publication Number Publication Date
WO2015110518A1 true WO2015110518A1 (fr) 2015-07-30

Family

ID=52394263

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2015/051226 WO2015110518A1 (fr) 2014-01-27 2015-01-22 Procédé de fermentation comprenant une culture de cellules à faible concentration en oxygène dissous

Country Status (3)

Country Link
AR (1) AR100302A1 (fr)
DE (1) DE102014201384A1 (fr)
WO (1) WO2015110518A1 (fr)

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109735474A (zh) * 2019-03-04 2019-05-10 西北大学 一株具有促生作用的氢氧化细菌zw-26及其分离方法和应用
US10329590B2 (en) 2014-05-13 2019-06-25 Evonik Degussa Gmbh Method of producing nylon
CN113337550A (zh) * 2021-08-02 2021-09-03 中国农业科学院农业环境与可持续发展研究所 农业废弃物发酵生产聚羟基脂肪酸酯的方法
US11124813B2 (en) 2016-07-27 2021-09-21 Evonik Operations Gmbh N-acetyl homoserine
US11174496B2 (en) 2015-12-17 2021-11-16 Evonik Operations Gmbh Genetically modified acetogenic cell

Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4601893A (en) 1984-02-08 1986-07-22 Pfizer Inc. Laminate device for controlled and prolonged release of substances to an ambient environment and method of use
EP0472869A2 (fr) 1990-08-30 1992-03-04 Degussa Ag Plasmides de corynebacterium glutamicum et vecteurs plasmidiques dérivés
WO1996015246A1 (fr) 1994-11-11 1996-05-23 Forschungszentrum Jülich GmbH Adn regulant l'expression genique dans des bacteries du genre corynebacterium
JPH10229891A (ja) 1997-02-20 1998-09-02 Mitsubishi Rayon Co Ltd マロン酸誘導体の製造法
DE10031999A1 (de) 1999-09-09 2001-04-19 Degussa Verfahren zur fermentativen Herstellung von D-Pantothensäure unter Verwendung coryneformer Bakterien
WO2011112154A1 (fr) * 2010-03-12 2011-09-15 G5 International Holdings Pte Ltd Production de polyhydroxyalcanoates
WO2012129450A1 (fr) * 2011-03-22 2012-09-27 Opx Biotechnologies, Inc. Production microbienne de produits chimiques, et compositions, procédés et systèmes associés

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE102006017760A1 (de) 2006-03-24 2007-09-27 Ufz-Umweltforschungszentrum Leipzig-Halle Gmbh Verfahren zur enzymatischen Herstellung von 2-Hydroxy-2-methylcarbonsäuren

Patent Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4601893A (en) 1984-02-08 1986-07-22 Pfizer Inc. Laminate device for controlled and prolonged release of substances to an ambient environment and method of use
EP0472869A2 (fr) 1990-08-30 1992-03-04 Degussa Ag Plasmides de corynebacterium glutamicum et vecteurs plasmidiques dérivés
WO1996015246A1 (fr) 1994-11-11 1996-05-23 Forschungszentrum Jülich GmbH Adn regulant l'expression genique dans des bacteries du genre corynebacterium
JPH10229891A (ja) 1997-02-20 1998-09-02 Mitsubishi Rayon Co Ltd マロン酸誘導体の製造法
DE10031999A1 (de) 1999-09-09 2001-04-19 Degussa Verfahren zur fermentativen Herstellung von D-Pantothensäure unter Verwendung coryneformer Bakterien
WO2011112154A1 (fr) * 2010-03-12 2011-09-15 G5 International Holdings Pte Ltd Production de polyhydroxyalcanoates
WO2012129450A1 (fr) * 2011-03-22 2012-09-27 Opx Biotechnologies, Inc. Production microbienne de produits chimiques, et compositions, procédés et systèmes associés

Non-Patent Citations (32)

* Cited by examiner, † Cited by third party
Title
"Vectors : a survey of molecular cloning vectors and their uses", 1988, BUTTERWORTH, pages: 179 - 204
DONAHUE ET AL., JOURNAL OF BACTERIOLOGY, vol. 182, no. 19, 2000, pages 5624 - 5627
DUNICAN; SHIVNAN, BIO/TECHNOLOGY, vol. 7, 1989, pages 1067 - 1070
EIKMANNS ET AL., GENE, vol. 102, 1991, pages 93 - 98
FREEDBERG ET AL., JOURNAL OF BACTERIOLOGY, vol. 115, no. 3, 1973, pages 816 - 823
GLOVER, D. M.: "DNA cloning: a practical approach", vol. 1-111, 1985, IRL PRESS LTD.
GOEDDEL, D. V.: "Systems for heterologous gene expression", METHODS ENZYMOL., vol. 185, 1990, pages 3 - 7
GUERRERO ET AL., GENE, vol. 138, 1994, pages 35 - 41
HERMANN ET AL., ELECTOPHORESIS, vol. 22, 2001, pages 1712 - 23
HERMANN ET AL., ELECTROPHORESIS, vol. 22, 2001, pages 1712.23
JENSEN; HAMMER, BIOTECHNOLOGY AND BIOENGINEERING, vol. 58, 1998, pages 191 - 195
LABARRE ET AL., JOURNAL OF BACTERIOLOGY, vol. 175, 1993, pages 1001 - 1007
LEFEBVRE GILLES ET AL: "Effects of low dissolved-oxygen concentrations on poly-(3-hydroxybutyrate-co-3-hydroxyvalerate) production by Alcaligenes eutrophus", APPLIED AND ENVIRONMENTAL MICROBIOLOGY, vol. 63, no. 3, 1997, pages 827 - 833, XP002737400, ISSN: 0099-2240 *
LI ZHENG-JUN ET AL: "Microbial production of meso-2,3-butanediol by metabolically engineered Escherichia coli under low oxygen condition", APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, vol. 87, no. 6, August 2010 (2010-08-01), pages 2001 - 2009, XP002737401, ISSN: 1432-0614 *
LOHAUS ET AL., BIOSPEKTRUM, vol. 5, 1998, pages 32 - 39
LOHAUS; MEYER, BIOSPEKTRUM, vol. 5, 1989, pages 32 - 39
LOTTSPEICH, ANGEWANDTE CHEMIE, vol. 111, 1999, pages 2630 - 2647
MALUMBRES ET AL., GENE, vol. 134, 1993, pages 15 - 24
MARTIN ET AL., BIO/TECHNOLOGY, vol. 5, 1987, pages 137 - 146
RANJITA BISWAS ET AL: "Enhanced production of 2,3-butanediol by engineered Bacillus subtilis", APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, vol. 94, no. 3, 1 May 2012 (2012-05-01), pages 651 - 658, XP055023812, ISSN: 0175-7598, DOI: 10.1007/s00253-011-3774-5 *
RAY ET AL., JOURNAL OF BACTERIOLOGY, vol. 182, no. 8, 2000, pages 2277 - 2284
REINSCHEID ET AL., APPLIED AND ENVIRONMENTAL MICROBIOLOGY, vol. 60, 1994, pages 126 - 132
SAMBROOK ET AL.: "Molecular Cloning: a laboratory manual", 1989, COLD SPRING HARBOR LABORATORY PRESS
SAMBROOK, J.; FRITSCH, E. F.; MANIATIS, T.: "Molecular cloning: a laboratory manual", 1989, COLD SPRING HARBOR LABORATORY PRESS
SCHÄFER ET AL., APPLIED AND ENVIRONMENTAL MICROBIOLOGY, vol. 60, 1994, pages 756 - 759
SCHWARZER; PÜHLER, BIO/TECHNOLOGY, vol. 9, 1991, pages 84 - 87
SURESH ET AL.: "Techniques for oxygen transfer measurement in bioreactors: a review", J CHEM TECHNOL BIOTECHNOL, vol. 84, 2009, pages 1091 - 1103
TAUCH ET AL., FEMS MICROBIOLOGY LET-TERS, vol. 123, 1994, pages 343 - 347
THIERBACH ET AL., APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, vol. 29, 1988, pages 356 - 362
TSUCHIYA; MORINAGA, BIO/TECHNOLOGY, vol. 6, 1988, pages 428 - 430
WILHELM ET AL., LOW-PRESSURE SOLUBILITY OF GASES IN LIQUID WATER, 1977, pages 219 - 262
WILSON ET AL., JOURNAL OF BACTERIOLOGY, vol. 183, 2001, pages 2151 - 2155

Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US10329590B2 (en) 2014-05-13 2019-06-25 Evonik Degussa Gmbh Method of producing nylon
US11174496B2 (en) 2015-12-17 2021-11-16 Evonik Operations Gmbh Genetically modified acetogenic cell
US11124813B2 (en) 2016-07-27 2021-09-21 Evonik Operations Gmbh N-acetyl homoserine
CN109735474A (zh) * 2019-03-04 2019-05-10 西北大学 一株具有促生作用的氢氧化细菌zw-26及其分离方法和应用
CN113337550A (zh) * 2021-08-02 2021-09-03 中国农业科学院农业环境与可持续发展研究所 农业废弃物发酵生产聚羟基脂肪酸酯的方法
WO2023010787A1 (fr) * 2021-08-02 2023-02-09 中国农业科学院农业环境与可持续发展研究所 Procédé de production de polyhydroxyalcanoate par fermentation de déchets agricoles

Also Published As

Publication number Publication date
AR100302A1 (es) 2016-09-28
DE102014201384A1 (de) 2015-07-30

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